Cucsa.119450.2 (mRNA) Cucumber (Gy14) v1

BLAST of Cucsa.119450.2 vs. Swiss-Prot
Match: MSL8_ARATH (Mechanosensitive ion channel protein 8 OS=Arabidopsis thaliana GN=MSL8 PE=2 SV=2)

HSP 1 Score: 885.6 bits (2287), Expect = 4.6e-256
Identity = 492/962 (51.14%), Postives = 641/962 (66.63%), Query Frame = 1

Query: 1   MDFSLKSFKSHPSYKYVRKLSGADATQDHEHLPILSDHQ--HSIMAVSDSSDRKEVIVKV 60
           MDF   SFKSH SYK +R   G  +    EHLPIL DH   HS M V D         K 
Sbjct: 1   MDFR-NSFKSHSSYKQIRS-PGDQSEPSPEHLPILHDHHPDHSGMVVDDQ--------KP 60

Query: 61  DEADASTLRATDLVNG-SGTIWRESSYDFWNDSDNRRNYGEGGAKTTD------------ 120
           D   +S      L +G +  + R++SY FW D+    +      +T+D            
Sbjct: 61  DSTRSS------LDDGRNAPVERDASYKFWQDNTTGTSTDHTAVRTSDKDPIAISRKGDR 120

Query: 121 ---DFEFRQHRKDVEDPPSKLI-GQFLHKQ---KASGEMSLDMDMEMLELPQDKTPLSTV 180
               F+F   +  V++ P+K++ G+ +++Q   + + E++LD+D E  ++     P  T 
Sbjct: 121 LSGSFDFVHGKLPVDESPTKMVAGEPVNRQWRGRNNEEITLDVDQENDDVSHQTMPTPTS 180

Query: 181 AESPMRRSSRELKVSFE-----------------SISEISENDSMRRRHRDSPLDEEHRG 240
                  +SRE++VSF                  S S  S + +MR       L EE   
Sbjct: 181 TARTSFDASREMRVSFNVRRAGGAFVAGSVPSSSSHSSSSSSATMRTNQDQPQLQEE--- 240

Query: 241 QQPRQCDRRAHGSNGEDDGAAEVLRCSSNSFFQRDVSFQRKSSLL-RAKTKSRLLDPP-E 300
                                EV+RC+SN      +SFQRKS L+ R KT+SRL DPP E
Sbjct: 241 ---------------------EVVRCTSN------MSFQRKSELISRVKTRSRLQDPPRE 300

Query: 301 HQDRRSGRVPKSGQVRSGLISKALDEEDDDPFLEEDLPDEYKKANLGVLTLLQWASLILI 360
            +   SG   +SGQ++SGL++    +E+DDP  EED+PDEYK+  L  +TLLQW SL+ I
Sbjct: 301 EETPYSGW--RSGQLKSGLLADI--DEEDDPLAEEDVPDEYKRGKLDAITLLQWLSLVAI 360

Query: 361 IAALVCTLTIRYWRRKKLWKLEVWKWEVMILVLICGRLVSGWGIRVIVFFIERNFLLRKR 420
           IAAL C+L+I+ W++ ++W L +WKWEV +LVLICGRLVSGWGIR++VFFIERNFLLRKR
Sbjct: 361 IAALACSLSIQSWKKVRVWNLHLWKWEVFLLVLICGRLVSGWGIRIVVFFIERNFLLRKR 420

Query: 421 VLYFVYGVRKAVQNCLWLGLVLIAWNFLFDDKVQREVKSNALEYVTKVLVCLLVSTLVWL 480
           VLYFVYGVR+AVQNCLWLGLVL+AW+FLFD KVQRE +S  L YVTK+LVC L+ST++WL
Sbjct: 421 VLYFVYGVRRAVQNCLWLGLVLLAWHFLFDKKVQRETRSRFLPYVTKILVCFLLSTILWL 480

Query: 481 VKTLMVKVLASSFHVSTYFDRIQDALFNQYVIETLSGPPLIEIQKNEEEEERLAEEVIKL 540
           +KTL+VKVLASSFHVSTYFDRIQ+ALFNQYVIETLSGPP+IE+ + EEEEER  +E+ K+
Sbjct: 481 IKTLVVKVLASSFHVSTYFDRIQEALFNQYVIETLSGPPMIEMSRIEEEEERAQDEIFKM 540

Query: 541 QNAGATIPPDLKATAFSTAQKGGRVIGSGGLQKSPRGRSGKLSRTLSKKGGDEGITIDHL 600
           QNAGA +PPDL A AF    K GRV+              KLS  + K   D GI+++HL
Sbjct: 541 QNAGANLPPDLCAAAFPPG-KSGRVMNP------------KLSPIIPKSTTDNGISMEHL 600

Query: 601 HKLSPKNVSAWNMKRLMNIVRHGTLSTLDEQIKDTAHEDESTTEIKSEYEAKVAAKKIFL 660
           H+++ KN+SAWNMKRLM IVR+ +L+TLDEQ+ ++ +EDEST +I+SE EAK AA+KIF 
Sbjct: 601 HRMNHKNISAWNMKRLMKIVRNVSLTTLDEQMLESTYEDESTRQIRSEKEAKAAARKIFK 660

Query: 661 NVARNGSKYIYLEDLMRFMEKDEASKTMGLFEGACESRKISKSSLKNWVVNAFRERRALA 720
           NV + G+KYIYLEDLMRF+ +DEA KTMGLFEGA E+++ISKS+LKNW+VNAFRERRALA
Sbjct: 661 NVEQRGAKYIYLEDLMRFLREDEAMKTMGLFEGAPENKRISKSALKNWLVNAFRERRALA 720

Query: 721 LTLNDTKT---------------------------AVNKLHRM----------------- 780
           LTLNDTKT                           A +K+                    
Sbjct: 721 LTLNDTKTAVNKLHHMINIVTAIVIVVIWLVLLEIASSKVLLFVSSQVVLLAFIFGNTVK 780

Query: 781 TVFESIIFLFVMHPFDVGDRCEIDGVQMIVEEMNILTTIFLRYDNQKIIFPNSVLATKAI 840
           TVFESIIFLF++HP+DVGDRCEID VQ++VEEMNILTT+FLRYDN KI++PNS+L  K+I
Sbjct: 781 TVFESIIFLFIVHPYDVGDRCEIDSVQLVVEEMNILTTVFLRYDNLKIMYPNSLLWQKSI 840

Query: 841 HNFYRSPDMGDGIEFCLHISTPPEKIAIMRQRIISYIEGKKEHWCPAPMIVLKDVEELNR 878
           +N+YRSPDMGD IEFC+HI+TP EKI++++QRI +YI+ K E+W P   I++KD+E+L+ 
Sbjct: 841 NNYYRSPDMGDAIEFCVHITTPLEKISVIKQRISNYIDNKPEYWYPQAKIIVKDLEDLHI 899

BLAST of Cucsa.119450.2 vs. Swiss-Prot
Match: MSL7_ARATH (Mechanosensitive ion channel protein 7 OS=Arabidopsis thaliana GN=MSL7 PE=3 SV=1)

HSP 1 Score: 767.7 bits (1981), Expect = 1.4e-220
Identity = 437/920 (47.50%), Postives = 585/920 (63.59%), Query Frame = 1

Query: 6   KSFKSHPSYKYVRKLSGADATQDHEHLPILSDHQHSIMAVSDSSDRKEVIVKVDEADAST 65
           K FKSH SYK +  +S  D                     ++ + +K+ +  +D+ D + 
Sbjct: 5   KPFKSHSSYKQI--ISTGDQ--------------------NEKTKKKKKLANLDDGDIAK 64

Query: 66  LRATDLVNGSGTIWRESSYDFWNDSDNRRNYGEGGAKTTDDFEFRQHRKDVEDPPSKLIG 125
            ++      SG+ +  +SY FW D     +Y + G+     F+F Q+R+           
Sbjct: 65  TQS------SGSSFDGNSYKFWQDIATD-DYTKSGS-----FDFPQYRE----------- 124

Query: 126 QFLHKQKASGEMSLDMDMEMLELPQDKTPLSTVAESPMRRSSRELKVSFESISEISENDS 185
                     E++LD++ E  E          V+ +     S+E +V F+  S  + N S
Sbjct: 125 ----------EITLDVNEETEETED-------VSNNNNLSGSKETRVFFKINSSGTNNMS 184

Query: 186 MRRRHRDSPLDEEHRGQQPRQCDRRAHGSNGEDDGAAEVLRCSSNSFFQRDVSFQRKSSL 245
              R   S                R +     +D    V+RCSS           RK+ L
Sbjct: 185 GSVRSCTSSTSFS-------SATMRLNLEQQLEDEGEVVVRCSS----------VRKTEL 244

Query: 246 L-RAKTKSRLLDPPEHQDRR-SGRVPKSGQVRSGLISKALD--EEDDDPFLEEDLPDEYK 305
           + RAK +SRL+DPP+ ++++ S  +  S Q+RSGL+ +  D  EE+DD   EED+P EY+
Sbjct: 245 VSRAKARSRLIDPPQEEEQQYSSWIGTSDQLRSGLLGRHSDDIEEEDDSSAEEDVPVEYR 304

Query: 306 KANLGVLTLLQWASLILIIAALVCTLTIRYWRRKKLWKLEVWKWEVMILVLICGRLVSGW 365
           K  +  +TLLQW SLI ++ ALV +L +  WR   LW L +WKWEV++LVLICGRLVSG 
Sbjct: 305 KLKMDAITLLQWMSLIALVVALVLSLGLHTWRNATLWSLHLWKWEVVLLVLICGRLVSGC 364

Query: 366 GIRVIVFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLFDDKVQREVKSNAL 425
           GIR+IVFFIERNFLLRKRVLYFVYGV+ AVQNCLWLGLVL+AW+FLFD KV++E +S+ L
Sbjct: 365 GIRIIVFFIERNFLLRKRVLYFVYGVKTAVQNCLWLGLVLLAWHFLFDKKVEKETQSDVL 424

Query: 426 EYVTKVLVCLLVSTLVWLVKTLMVKVLASSFHVSTYFDRIQDALFNQYVIETLSGPPLIE 485
             ++K+LVC L+ST++WL+KTL+VKVLASSFHVSTYFDRIQ+ALF+ Y+IETLSGPP++E
Sbjct: 425 LLMSKILVCFLLSTVLWLIKTLVVKVLASSFHVSTYFDRIQEALFHHYLIETLSGPPMLE 484

Query: 486 IQKNEEEEERLAEEVIKLQNAGATIPPDLKATAFSTAQKGGRVIGSGGLQKSPRGRSGKL 545
           + + EEEE+R  +E+ K+Q  GA + P+L + AF   + G  +             + K 
Sbjct: 485 LSRIEEEEDRTQDEIYKMQKGGADLSPELCSAAFPQEKSGSTM-------------NMKF 544

Query: 546 SRTLSKKGGDEGITIDHLHKLSPKNVSAWNMKRLMNIVRHGTLSTLDEQIKDTAHEDEST 605
           S  + K G D GIT+D LHK++ KNVSAWNMKRLM IVR+ +LSTLDEQ      EDEST
Sbjct: 545 SPIIPKTGSDNGITMDDLHKMNQKNVSAWNMKRLMKIVRNVSLSTLDEQALQNTCEDEST 604

Query: 606 TEIKSEYEAKVAAKKIFLNVARNGSKYIYLEDLMRFMEKDEASKTMGLFEGACESRKISK 665
            +I+SE EAK AA+KIF NVA+ G+K+IYLEDLMRF+  DEA KTM LFEGA  ++KI+K
Sbjct: 605 RQIRSEKEAKAAARKIFKNVAQPGTKHIYLEDLMRFLRVDEAMKTMCLFEGALVTKKITK 664

Query: 666 SSLKNWVVNAFRERRALALTLND---------------------------TKTAVNKL-- 725
           S+LKNW+VNAFRERRALALTLND                            + A +K   
Sbjct: 665 SALKNWLVNAFRERRALALTLNDTKTAVNKLHHMISFLTAIVIIVIWLILLEIATSKYLL 724

Query: 726 ---------------HRMTVFESIIFLFVMHPFDVGDRCEIDGVQMIVEEMNILTTIFLR 785
                             TVFESIIFLF++HP+DVGDR  ID V+M+VEEMNILTT+FLR
Sbjct: 725 FLTSQVVLLAFMFGNSLKTVFESIIFLFIIHPYDVGDRLLIDTVEMVVEEMNILTTVFLR 784

Query: 786 YDNQKIIFPNSVLATKAIHNFYRSPDMGDGIEFCLHISTPPEKIAIMRQRIISYIEGKKE 845
            DN KI++PN +L  KAIHN+ RSPDMGD +  C+HI+TPPEKIA ++QRI SYI+ K E
Sbjct: 785 ADNLKIVYPNILLWQKAIHNYNRSPDMGDEVTCCVHITTPPEKIAAIKQRISSYIDSKPE 832

Query: 846 HWCPAPMIVLKDVEELNRMRIAIWLTHRMNHQDMGERWTRRALLVEELVKIFQELDLQYR 878
           +W P   +++KDVE+LN +RIAIWL H++NHQ+MGER+TRRALL+EE++KI  ELD+QYR
Sbjct: 845 YWYPKADVIVKDVEDLNIVRIAIWLCHKINHQNMGERFTRRALLIEEVIKILLELDIQYR 832

BLAST of Cucsa.119450.2 vs. Swiss-Prot
Match: MSL5_ARATH (Mechanosensitive ion channel protein 5 OS=Arabidopsis thaliana GN=MSL5 PE=2 SV=1)

HSP 1 Score: 636.3 bits (1640), Expect = 4.8e-181
Identity = 360/700 (51.43%), Postives = 467/700 (66.71%), Query Frame = 1

Query: 43  MAVSDSSDRKEVIVKVDEADASTLRATDLVNGS--GTIWRESSYDFWNDSDNRRNYGEGG 102
           MA  DS+DR++ IV ++  ++  + AT   + +  G IW+ESSYDFW D +  +N  +G 
Sbjct: 1   MAAVDSTDRRDFIVNINGQESGAVGATGSSSNAEGGNIWKESSYDFW-DGEKGKNDKKGD 60

Query: 103 AKTTD--DFEFRQHRK------DVEDPPSKLIGQFLHKQKASG-EMSLDMDMEMLELPQD 162
            +  D   F FRQ  +      ++ DPPSKLIGQFLHKQ+ASG E+SLD+++ M EL  +
Sbjct: 61  DEDEDGGSFHFRQRGERRHSSAELSDPPSKLIGQFLHKQRASGDEISLDVELNMAELQSN 120

Query: 163 KTPLSTVAESPMRRSSRELKVSFESISEISENDSMRRRHRDSPL----DEEHRGQQPRQC 222
             P    A +  RR    +  S   +    + D++RRR   + L    DEE R +     
Sbjct: 121 TPPRPATASNTPRRGLTTISESSSPVKTKVKADAVRRRQNRTSLGGSSDEEGRNRDE--- 180

Query: 223 DRRAHGSNGEDDGAAEVLRCSSNSFFQRDVSFQRKSSLLRAKTKSRLLDPP-------EH 282
                         AEVL+C S           +K  L R KTKSRL DPP       + 
Sbjct: 181 --------------AEVLKCGS-----------KKPMLSRNKTKSRLQDPPTPTHPAIDK 240

Query: 283 QDRRSGRVPKSGQVRSGLISKAL-----------DEEDDDPFLEEDLPDEYKKANLGVLT 342
            + +SGR  +SG  +SG + K+            +EE++DPFL+EDLP+E+K+  L    
Sbjct: 241 TEMKSGR--RSGIFKSGFLGKSPKAGTPGRNGFEEEEEEDPFLDEDLPEEFKRDKLSFWV 300

Query: 343 LLQWASLILIIAALVCTLTIRYWRRKKLWKLEVWKWEVMILVLICGRLVSGWGIRVIVFF 402
            L+W SL+LI+ +LVC+LTI   +RK  WKL++WKWEV +LVLICGRLVS W +R+IVF 
Sbjct: 301 FLEWISLVLIVTSLVCSLTIHNLQRKTWWKLDLWKWEVTVLVLICGRLVSSWIVRIIVFL 360

Query: 403 IERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLFDDKVQREVKSNALEYVTKVLV 462
           +E+NF  RKRVLYFVYGVRK+VQNCLWLGLVL+AW+FLFD KV+RE +S AL YVT+VLV
Sbjct: 361 VEKNFTWRKRVLYFVYGVRKSVQNCLWLGLVLLAWHFLFDKKVERETRSTALRYVTRVLV 420

Query: 463 CLLVSTLVWLVKTLMVKVLASSFHVSTYFDRIQDALFNQYVIETLSGPPLIEIQKNEEEE 522
           CLLV+ ++WLVKT++VKVLASSFH+STYFDRIQ++LF QYVIETLSGPPL+EIQ+ EEEE
Sbjct: 421 CLLVALIIWLVKTILVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPLMEIQRMEEEE 480

Query: 523 ERLAEEVIKLQN-AGATIPPDLKATAFSTAQKGGRVIGSGGLQKSPRGRSGKLSRTLSKK 582
           +++AE+V  L+  AGA +PP LKAT  S  + G          KSP      L+R  SK+
Sbjct: 481 QQVAEDVKSLEKLAGAKLPPALKATVKSFMKVG----------KSP-----GLNRIGSKR 540

Query: 583 GGD-EGITIDHLHKLSPKNVSAWNMKRLMNIVRHGTLSTLDEQIKDTAHEDESTTEIKSE 642
           G D EGI ID L +++ KNVSAWNMKRLMNI+  G +STLD+ ++DT  EDE  T I+SE
Sbjct: 541 GEDGEGIRIDQLKRMNTKNVSAWNMKRLMNIILKGAISTLDQNMQDTTQEDEDATHIRSE 600

Query: 643 YEAKVAAKKIFLNVARNGSKYIYLEDLMRFMEKDEASKTMGLFEGACESRKISKSSLKNW 702
           YEAK AA+KIF NV   GS+YIYLED +RF+ ++EA + M LFEGA ES KISKS LKNW
Sbjct: 601 YEAKCAARKIFHNVTEPGSRYIYLEDFLRFLCEEEAERAMALFEGASESDKISKSCLKNW 654

Query: 703 VVNAFRERRALALTLNDTKTAVNKLHR-MTVFESIIFLFV 707
           VV AFRERRALALTLNDTKTAV++LHR + V   II + +
Sbjct: 661 VVKAFRERRALALTLNDTKTAVDRLHRIINVVIGIIIIII 654

HSP 2 Score: 283.1 bits (723), Expect = 1.0e-74
Identity = 127/175 (72.57%), Postives = 157/175 (89.71%), Query Frame = 1

Query: 696 TVFESIIFLFVMHPFDVGDRCEIDGVQMIVEEMNILTTIFLRYDNQKIIFPNSVLATKAI 755
           T+FE+IIFLFVMHPFDVGDRCEIDGVQ++VEEMNILTT+FLRYDNQKII+PNSVL TK I
Sbjct: 687 TIFEAIIFLFVMHPFDVGDRCEIDGVQLVVEEMNILTTVFLRYDNQKIIYPNSVLGTKPI 746

Query: 756 HNFYRSPDMGDGIEFCLHISTPPEKIAIMRQRIISYIEGKKEHWCPAPMIVLKDVEELNR 815
            N+YRSPDMGD +EFC+HI+TPPEKI  ++QRI+SY++ KK++W PAPMIV   +++LN 
Sbjct: 747 ANYYRSPDMGDAVEFCVHIATPPEKITAIKQRILSYVDNKKDYWYPAPMIVFLSMDDLNS 806

Query: 816 MRIAIWLTHRMNHQDMGERWTRRALLVEELVKIFQELDLQYRLLPLDINVRSLPP 871
           ++IA+WLTHRMNHQDMGER+ RR LL+EE+ K  +ELD++YRL PL+INVRSLPP
Sbjct: 807 VKIAVWLTHRMNHQDMGERYIRRGLLLEEVGKTCRELDIEYRLYPLNINVRSLPP 861

BLAST of Cucsa.119450.2 vs. Swiss-Prot
Match: MSL4_ARATH (Mechanosensitive ion channel protein 4 OS=Arabidopsis thaliana GN=MSL4 PE=3 SV=1)

HSP 1 Score: 620.2 bits (1598), Expect = 3.6e-176
Identity = 368/755 (48.74%), Postives = 486/755 (64.37%), Query Frame = 1

Query: 43  MAVSDSSDRKEVIVKVDEADASTLRATDLVNG-SGTIWRESSYDFW-NDSDNRRNYGEGG 102
           MAV  +  R++ +V++D  D          NG S   WRESS +FW ND  ++   GE  
Sbjct: 1   MAVDSTDQRRDFVVRIDGED----------NGDSEKFWRESSINFWHNDKSSKPPGGEED 60

Query: 103 AKTTDDFEFRQHRKDVEDPPSKLIGQFLHKQKASG-EMSLDMDMEMLELPQDKTP-LSTV 162
             + D       + +  DPPSKLI QFL+KQKASG E+SLDM+  M EL ++  P LS+ 
Sbjct: 61  DGSFDFMRRSSEKSEEPDPPSKLINQFLNKQKASGDEISLDMEANMPELQKNTVPPLSST 120

Query: 163 AESPMRRSSRELKVSFESISEISENDSMRRRHRDSPLDEEHR-GQQPRQCDRRAHGSNGE 222
           A S    S+  +     +       D++RRR     L    + G      + R  GS   
Sbjct: 121 AVSG---SASPVTAPVTASYRNGTGDAIRRRQNRVTLSPSVKDGDSSEDEENRVDGS--- 180

Query: 223 DDGAAEVLRCSSNSFFQRDVSFQRKSSLLRAKTKSRLLDPPE--HQDRRSGRVPKSGQVR 282
                EV++C+SN       S  R  +L++ KT+SRL+DPP   + D  SGR P+SG + 
Sbjct: 181 -----EVVKCTSNR------STMRTKTLMKMKTRSRLMDPPTPTYPDMVSGRTPRSGNLN 240

Query: 283 SGLISKALD------------EEDDDPFLEEDLPDEYKKANLGVLTLLQWASLILIIAAL 342
            G   +               EE++DPF EEDLP+  +K  + V  +++W  LILIIA+L
Sbjct: 241 PGFSGRNTKPGTPNQGGSKDLEEEEDPFSEEDLPEGLRKEKICVWVIIEWIFLILIIASL 300

Query: 343 VCTLTIRYWRRKKLWKLEVWKWEVMILVLICGRLVSGWGIRVIVFFIERNFLLRKRVLYF 402
           +C+L I Y R K LW L +WKWEVM+LVLICGRLVS W +++ V+F+E NFL RK+VLYF
Sbjct: 301 ICSLVIPYLRGKTLWDLALWKWEVMVLVLICGRLVSSWIVKLFVYFVESNFLWRKKVLYF 360

Query: 403 VYGVRKAVQNCLWLGLVLIAWNFLFDDKVQREVKSNALEYVTKVLVCLLVSTLVWLVKTL 462
           VYG+RK VQNCLWLGLVLIAW+FLFD KV+RE++S  L+YVTKVL+CLLV+ ++WL+KTL
Sbjct: 361 VYGIRKPVQNCLWLGLVLIAWHFLFDKKVEREMRSTVLKYVTKVLICLLVAVIIWLIKTL 420

Query: 463 MVKVLASSFHVSTYFDRIQDALFNQYVIETLSGPPLIEIQKNEEEEERLAEEVIKLQNAG 522
           +VKVLASSFH+STYFDRIQ++LF QYVIETLSGPP IEI     EEE++A +V   +  G
Sbjct: 421 LVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPRIEI---HIEEEKVANDVKTFEIVG 480

Query: 523 ATIPPDLKATAFSTAQKGGRVIGSGGLQKSPR--GRSGKLSRTLSKK-GGDEGITIDHLH 582
             + P L   A S+  +    +GSG LQKSP   G+S  LSR+ SKK GG+EGI IDHL 
Sbjct: 481 RKLSP-LGPKAVSSPPQ--VTVGSGRLQKSPSRVGKSPVLSRSGSKKEGGEEGIRIDHLQ 540

Query: 583 KLSPKNVSAWNMKRLMNIVRHGTLSTLDEQIKD-TAHEDESTTEIKSEYEAKVAAKKIFL 642
           +++ KNVSAW MK+LMN+++ GTLSTLDEQI+D T  ED+  T+I+SE+EAK+AA+KIF 
Sbjct: 541 RMNTKNVSAWKMKKLMNVIKKGTLSTLDEQIQDTTTQEDDKATQIRSEFEAKLAARKIFQ 600

Query: 643 NVARNGSKYIYLEDLMRFMEKDEASKTMGLFEGACESRKISKSSLKNWVVNAFRERRALA 702
           NVA  GS+YIY+ED MRF+ +DE+ + M LFEGA E  KISKS LKNWVVNAFRERRALA
Sbjct: 601 NVAEPGSRYIYMEDFMRFLSEDESERAMDLFEGASECHKISKSCLKNWVVNAFRERRALA 660

Query: 703 LTLNDTKTAVNKLHRMT-VFESIIFLFVMHPFDVGDRCEIDGVQ----MIVEEMNILTTI 762
           LTLNDTKTAVN+LHR+  V  SI+ L +           I G+     ++V    +L  +
Sbjct: 661 LTLNDTKTAVNRLHRIVDVLVSIVILIIW--------LLILGIATTKFLLVISSQLLLVV 709

Query: 763 FLRYDNQKIIFPNSVLATKAIHNFYRSPDMGDGIE 770
           F+  ++ K IF  +V+    +H F    D+GD  E
Sbjct: 721 FVFGNSCKTIF-EAVIFVFVMHPF----DVGDRCE 709

HSP 2 Score: 264.6 bits (675), Expect = 3.8e-69
Identity = 120/185 (64.86%), Postives = 157/185 (84.86%), Query Frame = 1

Query: 696 TVFESIIFLFVMHPFDVGDRCEIDGVQMIVEEMNILTTIFLRYDNQKIIFPNSVLATKAI 755
           T+FE++IF+FVMHPFDVGDRCEIDGVQMIVEEMNILTT+FLR+DNQKI++PNS+L TK I
Sbjct: 688 TIFEAVIFVFVMHPFDVGDRCEIDGVQMIVEEMNILTTVFLRFDNQKIVYPNSLLGTKPI 747

Query: 756 HNFYRSPDMGDGIEFCLHISTPPEKIAIMRQRIISYIEGKKEHWCPAPMIVLKDVEELNR 815
            N+YRSPDM D IEF +HI+TPPEK   +RQRI+SY++ KK+HW P+PMIV +D+  LN 
Sbjct: 748 ANYYRSPDMQDAIEFFVHIATPPEKTTALRQRILSYVDNKKDHWHPSPMIVFRDMCGLNS 807

Query: 816 MRIAIWLTHRMNHQDMGERWTRRALLVEELVKIFQELDLQYRLLPLDINVRSLP---PVN 875
           ++IA+W TH+MNHQ+MGER+ RR  L+EE+ ++ +ELD++YRL PL+INV+SLP   P+ 
Sbjct: 808 VKIAMWPTHKMNHQNMGERYVRRGQLLEEIGRLCRELDIEYRLYPLNINVKSLPAATPIT 867

Query: 876 STNLP 878
           S  +P
Sbjct: 868 SDRIP 872

BLAST of Cucsa.119450.2 vs. Swiss-Prot
Match: MSL6_ARATH (Mechanosensitive ion channel protein 6 OS=Arabidopsis thaliana GN=MSL6 PE=1 SV=1)

HSP 1 Score: 595.1 bits (1533), Expect = 1.2e-168
Identity = 331/596 (55.54%), Postives = 416/596 (69.80%), Query Frame = 1

Query: 193 SPLDEEHRGQQPRQ-CDRRAHGSNGEDDGAAEVLRCSSNSFFQRDVSFQRKSS-LLRAKT 252
           +P+ E  R    R    RR   SN  ++   EV++CS N+        QR SS LL+ +T
Sbjct: 119 TPVSESPRVSTKRDPVGRRDSRSNTNNNDDGEVVKCSGNN-----APIQRSSSTLLKMRT 178

Query: 253 KSRLLDPPEHQ------DRRSGRVPKSGQVRSGLISKAL----DEEDDDPFLEEDLPDEY 312
           +SRL DPP  Q      D +SGR+PKSGQ++SG   K+     +EE+DDPF  EDLP+EY
Sbjct: 179 RSRLSDPPTPQLPPQTADMKSGRIPKSGQMKSGFFGKSPKTQGEEEEDDPFAAEDLPEEY 238

Query: 313 KKANLGVLTLLQWASLILIIAALVCTLTIRYWRRKKLWKLEVWKWEVMILVLICGRLVSG 372
           +K  L +  +L+W SLILIIA  VCTL I   R+KKLW+L++WKWE M+LVLICGRLVS 
Sbjct: 239 RKDKLSLWIVLEWLSLILIIAGFVCTLAIPSLRKKKLWELQLWKWESMVLVLICGRLVSS 298

Query: 373 WGIRVIVFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLFDDKVQREVKSNA 432
           W ++++VFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGLVL+AW+FLFD+KV +   + A
Sbjct: 299 WIVKIVVFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLLAWHFLFDEKVAKAANTKA 358

Query: 433 LEYVTKVLVCLLVSTLVWLVKTLMVKVLASSFHVSTYFDRIQDALFNQYVIETLSGPPLI 492
           L  VTK+ VCLLV  L+WLVKTL+VKVLASSFH+STYFDRIQ++LF QYVIETLSGPPLI
Sbjct: 359 LRVVTKIFVCLLVGFLLWLVKTLLVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPLI 418

Query: 493 EIQKNEEEEERLAEEVIKLQNAGATIPPDLKATAFSTAQKGGRVIGSGGLQKSP--RGRS 552
           EIQKNEEEEER++ EV K QN G                    V    G QKSP   G+S
Sbjct: 419 EIQKNEEEEERISVEVKKFQNPGG-------------------VEIQSGAQKSPMKTGKS 478

Query: 553 GKLSRTLSKKGG----DEGITIDHLHKLSPKNVSAWNMKRLMNIVRHGTLSTLDEQIKDT 612
             LS  LS  GG    ++GITID LHKL+PKNVSAW MKRLMNI+R+G+L+TLDEQ++D 
Sbjct: 479 PFLSHVLSNGGGGGGENKGITIDSLHKLNPKNVSAWKMKRLMNIIRNGSLTTLDEQLQDP 538

Query: 613 AHEDESTTEIKSEYEAKVAAKKIFLNVARNGSKYIYLEDLMRFMEKDEASKTMGLFEGAC 672
           + +D+   +I+SE+EAK+AA+KIF NVA+ GSK+IY  D+MRF+  DEA KT+ LFEGA 
Sbjct: 539 SLDDDKGNQIRSEFEAKLAARKIFHNVAKPGSKFIYANDIMRFLPDDEALKTLSLFEGAS 598

Query: 673 ESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMTVFESIIFLFVMHPFDVGD 732
           E+ +ISKSSLKNWVVNAFRERRALALTLNDTKTAVN+LH+M      I + V+    +G 
Sbjct: 599 ETNRISKSSLKNWVVNAFRERRALALTLNDTKTAVNRLHKMVNIVVGIIILVIWLIILG- 658

Query: 733 RCEIDGVQ-MIVEEMNILTTIFLRYDNQKIIFPNSVLATKAIHNFYRSPDMGDGIE 770
              I   + ++V    ++   F+  +  KI+F  S++    IH F    D+GD  E
Sbjct: 659 ---ITSTKFLVVMSSQVVVVAFIFGNMCKIVF-ESIIYLFVIHPF----DVGDRCE 681

HSP 2 Score: 288.5 bits (737), Expect = 2.5e-76
Identity = 134/181 (74.03%), Postives = 161/181 (88.95%), Query Frame = 1

Query: 697 VFESIIFLFVMHPFDVGDRCEIDGVQMIVEEMNILTTIFLRYDNQKIIFPNSVLATKAIH 756
           VFESII+LFV+HPFDVGDRCEIDGVQM+VEEMNILTT+FLR+DNQK+++PNS+L TK+I 
Sbjct: 661 VFESIIYLFVIHPFDVGDRCEIDGVQMVVEEMNILTTVFLRFDNQKVVYPNSLLWTKSIG 720

Query: 757 NFYRSPDMGDGIEFCLHISTPPEKIAIMRQRIISYIEGKKEHWCPAPMIVLKDVEELNRM 816
           N+YRSPDMGDGIEF +HI+TP EKI +++QRI SYIEGKK+HW PAPMIV KD+E LN +
Sbjct: 721 NYYRSPDMGDGIEFSIHITTPAEKIILIKQRITSYIEGKKDHWYPAPMIVFKDMESLNSV 780

Query: 817 RIAIWLTHRMNHQDMGERWTRRALLVEELVKIFQELDLQYRLLPLDINVRSLPPVNSTNL 876
           RIA+W THRMNHQDMGE+W RR+ LVEE+ KI +ELD++YRL PLDINVR+LP   ST L
Sbjct: 781 RIAVWPTHRMNHQDMGEKWARRSQLVEEIAKICRELDIEYRLYPLDINVRNLP--TSTAL 839

Query: 877 P 878
           P
Sbjct: 841 P 839

HSP 3 Score: 99.4 bits (246), Expect = 2.1e-19
Identity = 67/158 (42.41%), Postives = 88/158 (55.70%), Query Frame = 1

Query: 43  MAVSDSSDRKEVIVKVDEADASTLRATDLVNGS--GTIWRESSYDFWNDSDNRRNYGEGG 102
           MAV D++DR+EVIVK+D  + +     + V+G   G IWR+ SYDFW D +   N G   
Sbjct: 1   MAV-DAADRREVIVKIDGENGNN----NGVSGETVGKIWRDGSYDFWTDGEGNLNKGHNA 60

Query: 103 AKTTDD--------------FEFRQHRKDVEDPPSKLIGQFLHKQKASGEMSLDMDMEML 162
           A    D              FEFR+     EDPP+KLIGQFLHKQ+ASGE+ LDMD+ M 
Sbjct: 61  AAVDSDRSAATTGEQQKDEGFEFRRG----EDPPTKLIGQFLHKQQASGEICLDMDLGMD 120

Query: 163 ELPQDKTPLSTVAESPMRRSSRELKVSFESISEISEND 185
           EL      L+ V+ESP   + R+     +S S  + ND
Sbjct: 121 EL--QSRGLTPVSESPRVSTKRDPVGRRDSRSNTNNND 147

BLAST of Cucsa.119450.2 vs. TrEMBL
Match: A0A0A0KE62_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G124170 PE=4 SV=1)

HSP 1 Score: 1711.8 bits (4432), Expect = 0.0e+00
Identity = 872/923 (94.47%), Postives = 873/923 (94.58%), Query Frame = 1

Query: 1   MDFSLKSFKSHPSYKYVRKLSGADATQDHEHLPILSDHQHSIMAVSDSSDRKEVIVKVDE 60
           MDFSLKSFKSHPSYKYVRKLSGADATQDHEHLPILSDHQHSIMAVSDSSDRKEVIVKVDE
Sbjct: 1   MDFSLKSFKSHPSYKYVRKLSGADATQDHEHLPILSDHQHSIMAVSDSSDRKEVIVKVDE 60

Query: 61  ADASTLRATDLVNGSGTIWRESSYDFWNDSDNRRNYGEGGAKTTDDFEFRQHRKDVEDPP 120
           ADASTLRATDLVNGSGTIWRESSYDFWNDSDNRRNYGEGGAKTTDDFEFRQHRKDVEDPP
Sbjct: 61  ADASTLRATDLVNGSGTIWRESSYDFWNDSDNRRNYGEGGAKTTDDFEFRQHRKDVEDPP 120

Query: 121 SKLIGQFLHKQKASGEMSLDMDMEMLELPQDKTPLSTVAESPMRRSSRELKVSFESISEI 180
           SKLIGQFLHKQKASGEMSLDMDMEMLELPQDKTPLSTVAESPMRRSSRELKVSFESISEI
Sbjct: 121 SKLIGQFLHKQKASGEMSLDMDMEMLELPQDKTPLSTVAESPMRRSSRELKVSFESISEI 180

Query: 181 SENDSMRRRHRDSPLDEEHRGQQPRQCDRRAHGSNGEDDGAAEVLRCSSNSFFQRDVSFQ 240
           SENDSMRRRHRDSPLDEEHRGQQPRQCDRRAHGSNGEDDGAAEVLRCSSNSFFQRDVSFQ
Sbjct: 181 SENDSMRRRHRDSPLDEEHRGQQPRQCDRRAHGSNGEDDGAAEVLRCSSNSFFQRDVSFQ 240

Query: 241 RKSSLLRAKTKSRLLDPPEHQDRRSGRVPKSGQVRSGLISKALDEEDDDPFLEEDLPDEY 300
           RKSSLLRAKTKSRLLDPPEHQDRRSGRVPKSGQVRSGLISKALDEEDDDPFLEEDLPDEY
Sbjct: 241 RKSSLLRAKTKSRLLDPPEHQDRRSGRVPKSGQVRSGLISKALDEEDDDPFLEEDLPDEY 300

Query: 301 KKANLGVLTLLQWASLILIIAALVCTLTIRYWRRKKLWKLEVWKWEVMILVLICGRLVSG 360
           KKANLGVLTLLQWASLILIIAALVCTLTIRYWRRKKLWKLEVWKWEVMILVLICGRLVSG
Sbjct: 301 KKANLGVLTLLQWASLILIIAALVCTLTIRYWRRKKLWKLEVWKWEVMILVLICGRLVSG 360

Query: 361 WGIRVIVFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLFDDKVQREVKSNA 420
           WGIRVIVFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLFDDKVQREVKSNA
Sbjct: 361 WGIRVIVFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLFDDKVQREVKSNA 420

Query: 421 LEYVTKVLVCLLVSTLVWLVKTLMVKVLASSFHVSTYFDRIQDALFNQYVIETLSGPPLI 480
           LEYVTKVLVCLLVSTLVWLVKTLMVKVLASSFHVSTYFDRIQDALFNQYVIETLSGPPLI
Sbjct: 421 LEYVTKVLVCLLVSTLVWLVKTLMVKVLASSFHVSTYFDRIQDALFNQYVIETLSGPPLI 480

Query: 481 EIQKNEEEEERLAEEVIKLQNAGATIPPDLKATAFSTAQKGGRVIGSGGLQKSPRGRSGK 540
           EIQKNEEEEERLAEEVIKLQNAGATIPPDLKATAFSTAQKGGRVIGSGGLQKSPRGRSGK
Sbjct: 481 EIQKNEEEEERLAEEVIKLQNAGATIPPDLKATAFSTAQKGGRVIGSGGLQKSPRGRSGK 540

Query: 541 LSRTLSKKGGDEGITIDHLHKLSPKNVSAWNMKRLMNIVRHGTLSTLDEQIKDTAHEDES 600
           LSRTLSKKGGDEGITIDHLHKLSPKNVSAWNMKRLMNIVRHGTLSTLDEQIKDTAHEDES
Sbjct: 541 LSRTLSKKGGDEGITIDHLHKLSPKNVSAWNMKRLMNIVRHGTLSTLDEQIKDTAHEDES 600

Query: 601 TTEIKSEYEAKVAAKKIFLNVARNGSKYIYLEDLMRFMEKDEASKTMGLFEGACESRKIS 660
           TTEIKSEYEAKVAAKKIFLNVARNGSKYIYLEDLMRFMEKDEASKTMGLFEGACESRKIS
Sbjct: 601 TTEIKSEYEAKVAAKKIFLNVARNGSKYIYLEDLMRFMEKDEASKTMGLFEGACESRKIS 660

Query: 661 KSSLKNWVVNAFRERRALALTLNDTK---------------------------TAVNKLH 720
           KSSLKNWVVNAFRERRALALTLNDTK                            A +K  
Sbjct: 661 KSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVIILVIWLLILGIATSKFL 720

Query: 721 RM-----------------TVFESIIFLFVMHPFDVGDRCEIDGVQMIVEEMNILTTIFL 780
                              TVFESIIFLFVMHPFDVGDRCEIDGVQMIVEEMNILTTIFL
Sbjct: 721 LFVTSQLVLVAFVFGNTCKTVFESIIFLFVMHPFDVGDRCEIDGVQMIVEEMNILTTIFL 780

Query: 781 RYDNQKIIFPNSVLATKAIHNFYRSPDMGDGIEFCLHISTPPEKIAIMRQRIISYIEGKK 840
           RYDNQKIIFPNSVLATKAIHNFYRSPDMGDGIEFCLHISTPPEKIAIMRQRIISYIEGKK
Sbjct: 781 RYDNQKIIFPNSVLATKAIHNFYRSPDMGDGIEFCLHISTPPEKIAIMRQRIISYIEGKK 840

Query: 841 EHWCPAPMIVLKDVEELNRMRIAIWLTHRMNHQDMGERWTRRALLVEELVKIFQELDLQY 880
           EHWCPAPMIVLKDVEELNRMRIAIWLTHRMNHQDMGERWTRRALLVEELVKIFQELDLQY
Sbjct: 841 EHWCPAPMIVLKDVEELNRMRIAIWLTHRMNHQDMGERWTRRALLVEELVKIFQELDLQY 900

BLAST of Cucsa.119450.2 vs. TrEMBL
Match: W9QBV6_9ROSA (Mechanosensitive ion channel protein 8 OS=Morus notabilis GN=L484_006078 PE=4 SV=1)

HSP 1 Score: 1114.8 bits (2882), Expect = 0.0e+00
Identity = 613/966 (63.46%), Postives = 717/966 (74.22%), Query Frame = 1

Query: 1   MDFSLK-SFKSHPSYKYVRKLS--GADATQDHEHLPILSDHQ----HSIM---AVSD--- 60
           MDFSLK SFKSH SYK  RK+S  G +    HE LPILSDH     H +     ++D   
Sbjct: 1   MDFSLKKSFKSHGSYKNSRKISAGGGNPDNSHEELPILSDHDFDDNHHVQHYRRLNDNGK 60

Query: 61  ----SSDRKEVIVKVDEADASTLRATDLVNGSGTIWRESSYDFWNDSDNRRNYGEGGAKT 120
               S+ R EVIVK+DE       A+D    +   WRE+SY  W ++ N R+ G  G   
Sbjct: 61  MGGASTGRNEVIVKIDEGGNPPAAASDQSRNAENFWRENSYGAWKENANVRDGGGSG--- 120

Query: 121 TDDFEFRQHRKDVEDPPSKLIGQFLHKQKASGEMSLDMDMEMLELPQD-----KTPLSTV 180
            + F F Q     EDPPSKLI QFLH+QKASG++SLDMD+EM EL +      +  L+ V
Sbjct: 121 -ESFHFAQ---TTEDPPSKLIRQFLHRQKASGDISLDMDLEMEELRRSAGSGGELDLTPV 180

Query: 181 AESPMR-RSSRELKVSFESI-----SEISENDSMRRRHRDSPLDEEHRGQQPRQCDRRAH 240
           +ESP   RSSRELKVS         SEIS+ND++RRR +DSP D +         +RR  
Sbjct: 181 SESPQNSRSSRELKVSLHPSPSDVGSEISQNDAVRRRFKDSPNDNDE------SLERR-- 240

Query: 241 GSNGEDDGAAEVLRCSSNSFFQRDVSFQRKSSLLRAKTKSRLLDPPEHQDRRSGRVPKSG 300
               E DG  EV+RC+SN+ FQR++SFQR+SS LR KT+SRL DPPE  D RSGR+PKSG
Sbjct: 241 ----ERDGE-EVVRCTSNASFQREISFQRRSSFLRVKTRSRLQDPPEEPDMRSGRIPKSG 300

Query: 301 QV------------RSGLISKALDEEDDDPFLEEDLPDEYKKANLGVLTLLQWASLILII 360
           Q+            +SGL+ K  DEE+DDPF +EDLPDE+KKANL  LTLLQWASLILI+
Sbjct: 301 QIPRSGPIPRSGPMKSGLLGK--DEEEDDPFSDEDLPDEFKKANLSALTLLQWASLILIV 360

Query: 361 AALVCTLTIRYWRRKKLWKLEVWKWEVMILVLICGRLVSGWGIRVIVFFIERNFLLRKRV 420
               CTL+I Y R K L KL++WKWEV+ILVLICGRLVSGWGIR+ VFF ERNFLLRKRV
Sbjct: 361 GLFACTLSIHYLRHKNLLKLKLWKWEVLILVLICGRLVSGWGIRIAVFFFERNFLLRKRV 420

Query: 421 LYFVYGVRKAVQNCLWLGLVLIAWNFLFDDKVQREVKSNALEYVTKVLVCLLVSTLVWLV 480
           LYFVYGVRKAVQNCLWLGLVLIAW+FLFD +V+RE +S +L+YVTKVL+C LV TL+WLV
Sbjct: 421 LYFVYGVRKAVQNCLWLGLVLIAWHFLFDKRVERETRSESLKYVTKVLICFLVGTLLWLV 480

Query: 481 KTLMVKVLASSFHVSTYFDRIQDALFNQYVIETLSGPPLIEIQKNEEEEERLAEEVIKLQ 540
           KTL+VKVLASSFHVSTYFDRIQD+LFNQ+VIETLSGPPLIEI K EEEEERLA+EV KLQ
Sbjct: 481 KTLIVKVLASSFHVSTYFDRIQDSLFNQFVIETLSGPPLIEIYKTEEEEERLADEVEKLQ 540

Query: 541 NAGATIPPDLKATAFSTA-QKGGRVIGSGGLQKSPRGRSGKLSRTLSKKGGDEGITIDHL 600
           NAGATIPPDL   AFS+A  +  RVIGSG L ++   +S KLSR+LSK G DEGITIDHL
Sbjct: 541 NAGATIPPDL---AFSSALPRTSRVIGSGRLSRT--WKSSKLSRSLSKIG-DEGITIDHL 600

Query: 601 HKLSPKNVSAWNMKRLMNIVRHGTLSTLDEQIKDTAHEDESTTEIKSEYEAKVAAKKIFL 660
           HKL+PKNVSAWNMKRLM +VRHGTL+TLDEQI D+ HEDES T+I+SE EAK AAKKIF 
Sbjct: 601 HKLNPKNVSAWNMKRLMRMVRHGTLTTLDEQIVDSTHEDESATQIRSEVEAKAAAKKIFQ 660

Query: 661 NVARNGSKYIYLEDLMRFMEKDEASKTMGLFEGACESRKISKSSLKNWVVNAFRERRALA 720
           NVAR GSK+I LEDLMRFM +DEA KTM LFEGA ES++ISKSSLKNWVVNAFRERRALA
Sbjct: 661 NVARRGSKFICLEDLMRFMREDEAVKTMSLFEGASESQRISKSSLKNWVVNAFRERRALA 720

Query: 721 LTLNDTKTA---------------------------VNKLHRM----------------- 780
           LTLNDTKTA                            +K                     
Sbjct: 721 LTLNDTKTAVNKLHRIVNVIVAIVIGVIWLLILGIATSKFLLFISSQLVLVAFIFGNTCK 780

Query: 781 TVFESIIFLFVMHPFDVGDRCEIDGVQMIVEEMNILTTIFLRYDNQKIIFPNSVLATKAI 840
           TVFE+I+FLFVMHPFDVGDRCEIDGVQM+VEEMNILTT+FLRYDN KIIFPNSVLATKAI
Sbjct: 781 TVFEAIVFLFVMHPFDVGDRCEIDGVQMVVEEMNILTTVFLRYDNAKIIFPNSVLATKAI 840

Query: 841 HNFYRSPDMGDGIEFCLHISTPPEKIAIMRQRIISYIEGKKEHWCPAPMIVLKDVEELNR 878
            NFYRSPDMGDGIEF +H++TP +KIA MRQRI +Y+E KKEHW PAPM+++KD++ELNR
Sbjct: 841 DNFYRSPDMGDGIEFSIHVATPADKIAAMRQRITNYVENKKEHWYPAPMVIMKDLDELNR 900

BLAST of Cucsa.119450.2 vs. TrEMBL
Match: A0A0A0K619_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G017150 PE=4 SV=1)

HSP 1 Score: 1088.6 bits (2814), Expect = 0.0e+00
Identity = 578/942 (61.36%), Postives = 704/942 (74.73%), Query Frame = 1

Query: 1   MDFSLKSFKSHPSYKYVRKLS--GADATQDHEHLPILSDHQ---HSI-------MAVSDS 60
           MD   KSFK + S+K+ RK+S  GA +  +HE LPIL +HQ   HS        +  SD+
Sbjct: 1   MDTLKKSFKGNVSFKHTRKISAGGAGSEINHEELPILLNHQTTDHSRCLRDRHPVNDSDT 60

Query: 61  SDRKEVIVKVDEADASTLRATDLV---NGSGTIWRESSYDFWNDSD-----NRRNYGEGG 120
           SDR EVI+K+D   +S+  +  L    N  G +WRES YDFWN+ +     + R     G
Sbjct: 61  SDRTEVILKIDGGGSSSAVSRSLDSAGNNGGNVWRESRYDFWNNDEIGIGESARMGDNSG 120

Query: 121 AKTTDDFEFRQHRKDVEDPPSKLIGQFLHKQKASGEMSLDMDMEMLELPQDKTPLSTVAE 180
               + FEF Q    +EDPP+KLIG FL KQK  GE +LDMD+EM EL  ++  +  +AE
Sbjct: 121 VDRNEGFEFVQTGYGMEDPPTKLIGDFLCKQKIEGETTLDMDLEMEELKPNRI-VPPLAE 180

Query: 181 SPMRRSSRELKVSFESIS-EISEND-SMRRRHRDS-PLDEEHRG-QQPRQCDRRAHG--- 240
           SP+ ++S++LKVSF+  S EIS ND S+RRR+RDS  L EE +G Q PRQ          
Sbjct: 181 SPLSQTSKDLKVSFQHDSTEISSNDQSIRRRNRDSNDLKEESKGGQSPRQQPHHERLGSP 240

Query: 241 --SNGEDDGAAEVLRCSSNSFFQRDVSFQRKSSLLRAKTKSRLLDPPEHQDRRSGRVPKS 300
             S  +++  AE +RC+SN  F  ++SFQRKS+LLRAKTKSRL+DPP   DR SG +PKS
Sbjct: 241 TISGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLIDPPAEPDRLSGLIPKS 300

Query: 301 GQVRSGLISKALDEEDDDPFLEEDLPDEYKKANLGVLTLLQWASLILIIAALVCTLTIRY 360
           GQ+RSG + K ++++DDDPFLE+DLPDE+K+ N   LT+LQW SLI+I AAL+CTL++ Y
Sbjct: 301 GQLRSGFLGK-IEDDDDDPFLEDDLPDEFKRGNFSALTVLQWVSLIIITAALICTLSVPY 360

Query: 361 WRRKKLWKLEVWKWEVMILVLICGRLVSGWGIRVIVFFIERNFLLRKRVLYFVYGVRKAV 420
            R K LW+L++WKWEVMI +LICGRLVSGWGIR+ VFFIERNFLLRKRVLYFVYGVRK V
Sbjct: 361 LREKSLWELDIWKWEVMIFILICGRLVSGWGIRIGVFFIERNFLLRKRVLYFVYGVRKPV 420

Query: 421 QNCLWLGLVLIAWNFLFDDKVQREVKSNALEYVTKVLVCLLVSTLVWLVKTLMVKVLASS 480
           QNCLWLGLVLIAW+ LF+ +V+++  ++ L YV++VLVCLL+STL+WLVKTLMVKVLASS
Sbjct: 421 QNCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKVLASS 480

Query: 481 FHVSTYFDRIQDALFNQYVIETLSGPPLIEIQKNEEEEERLAEEVIKLQNAGATIPPDLK 540
           FHVSTYFDRIQ++LFNQYVIETLSGPPL+EI+KNEEEEER+A+EV KLQNAG  IPPDLK
Sbjct: 481 FHVSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGIIIPPDLK 540

Query: 541 ATAFSTAQKGGRVIGSGGLQKSPRGRSGKLSRTLSKKGGDEGITIDHLHKLSPKNVSAWN 600
           A  F++  K GR IGSG   KS   +S KLSR L+K   D GITIDHLHKLS KNVSAWN
Sbjct: 541 AATFASI-KSGREIGSGRTHKSFCAKSCKLSRALTKNRND-GITIDHLHKLSTKNVSAWN 600

Query: 601 MKRLMNIVRHGTLSTLDEQIKDTAHEDESTTEIKSEYEAKVAAKKIFLNVARNGSKYIYL 660
           MKRL+NIVR+G++STLDEQI+    +DESTTEIKSE EAK AAKKIF NVA  G KYIYL
Sbjct: 601 MKRLLNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVAHRGYKYIYL 660

Query: 661 EDLMRFMEKDEASKTMGLFEGACESRKISKSSLKNWVVNAFRERRALALTLNDTKT---- 720
           +DL+RFM +DE  KTM LFEGA E+++ISKS+LKNWVVNAFRERRALALTLNDTKT    
Sbjct: 661 DDLVRFMREDEVLKTMSLFEGAAENQRISKSALKNWVVNAFRERRALALTLNDTKTAVDK 720

Query: 721 -----------------------AVNKLHRM-----------------TVFESIIFLFVM 780
                                  A +K                     T+FE+IIFLFVM
Sbjct: 721 LHHMVNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFVM 780

Query: 781 HPFDVGDRCEIDGVQMIVEEMNILTTIFLRYDNQKIIFPNSVLATKAIHNFYRSPDMGDG 840
           HPFDVGDRCEIDG+QM+VEEMNILTT+FLRYDN K+I PNSVLATK IHNFYRSPDMG+ 
Sbjct: 781 HPFDVGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVIIPNSVLATKLIHNFYRSPDMGES 840

Query: 841 IEFCLHISTPPEKIAIMRQRIISYIEGKKEHWCPAPMIVLKDVEELNRMRIAIWLTHRMN 870
           IEF +HI+TP EKI  M+ RIISYIEG KEHW PAPMIV KD++ LN++++A+WL+HRMN
Sbjct: 841 IEFLVHIATPAEKITAMKHRIISYIEGNKEHWYPAPMIVFKDIDGLNKVKLAVWLSHRMN 900

BLAST of Cucsa.119450.2 vs. TrEMBL
Match: K7LLZ5_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_10G284300 PE=4 SV=1)

HSP 1 Score: 1053.5 bits (2723), Expect = 1.4e-304
Identity = 572/934 (61.24%), Postives = 688/934 (73.66%), Query Frame = 1

Query: 6   KSFKSHPSYKYVRKLSGADATQ-DHEHLPILSDHQHSIMAVSDSSDRKEVIVKVDEADAS 65
           KSFKS+ SY    +  GA  T  +HE LPIL D Q    +   + D    +VK++E  + 
Sbjct: 6   KSFKSYGSYNKHSRFFGAGNTDPEHEQLPILLDQQTLRQSAMPAGD---YVVKINEDGSE 65

Query: 66  TLRATDLVNGSGTIWRESSYDFWN-DSDNRRNYGEGGAKTTDDFEFRQHRKDVEDPPSKL 125
             +          IWRESSY+FWN D+            + + F+FR      EDPPS+L
Sbjct: 66  APQ-------DNKIWRESSYEFWNNDTTTTTTTTTIPGSSEESFDFRHS----EDPPSQL 125

Query: 126 IGQFLHKQKASGEMSLDMDMEMLELPQ------DKTPLSTVAESPM-RRSSRELKVSFES 185
           IG+FLHKQ+ASGEM LDMD+EM EL +      D   L+ V ESPM  R SRELKVSFE 
Sbjct: 126 IGRFLHKQRASGEMQLDMDLEMEELQREGGDDDDDGKLTPVEESPMTHRVSRELKVSFEE 185

Query: 186 ISEI-----SENDSMRRRH-RDSP-LDEEHRGQQPRQCDRRAHGSNGEDDGAAEVLRCSS 245
            +       ++ND++RRRH +DSP L E  R  QP Q DRR   S     G  EV+RC+S
Sbjct: 186 PAYNVNFLETQNDAVRRRHSKDSPSLAEFQRPPQPPQYDRRRSPSPSPACGD-EVVRCTS 245

Query: 246 NSFFQRDVSFQRKSSLLRAKTKSRLLDPPEHQDRRSGRVPKSGQVRSGLISKALDEEDDD 305
           N+ F+R++S QRKS+LL+AKT+SRL+DPPE  DR+SGRV KSGQ+ SG + K  DEEDDD
Sbjct: 246 NASFERNLSMQRKSALLKAKTRSRLMDPPEEPDRKSGRVLKSGQLLSGFLGKKNDEEDDD 305

Query: 306 PFLEEDLPDEYKKANLGVLTLLQWASLILIIAALVCTLTIRYWRRKKLWKLEVWKWEVMI 365
           PFLEEDLPDE+K+ +  +  LL+W SLI II  L+ TL + + R K LW+L +WKWEVM+
Sbjct: 306 PFLEEDLPDEFKETHFSLWILLEWLSLISIIGLLITTLCVPFLRNKNLWQLRLWKWEVMV 365

Query: 366 LVLICGRLVSGWGIRVIVFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLFD 425
           LVLICGRLVS W +R+ VF IERNFLLRKRVLYFVYGVRKAVQNC+WLGLVLIAW+ LFD
Sbjct: 366 LVLICGRLVSDWVVRIAVFCIERNFLLRKRVLYFVYGVRKAVQNCVWLGLVLIAWHLLFD 425

Query: 426 DKVQREVKSNALEYVTKVLVCLLVSTLVWLVKTLMVKVLASSFHVSTYFDRIQDALFNQY 485
            +VQRE  S+ LEYVTKVLVC LV TLVWL+KTLMVKVLASSFHVSTYFDRIQ++LFNQ+
Sbjct: 426 KRVQRETHSDFLEYVTKVLVCFLVGTLVWLLKTLMVKVLASSFHVSTYFDRIQESLFNQF 485

Query: 486 VIETLSGPPLIEIQKNEEEEERLAEEVIKLQNAGATIPPDLKATAFSTAQKGGRVIGSGG 545
           VIETLSGPPL+EIQK EEEEERLA+EV KLQNAG TIPPDL+A+AFS  + G   + SG 
Sbjct: 486 VIETLSGPPLVEIQKAEEEEERLADEVQKLQNAGVTIPPDLRASAFSNIKSGR--LRSGM 545

Query: 546 LQKSPRGRSGKLSRTLSKKGGDEG--ITIDHLHKLSPKNVSAWNMKRLMNIVRHGTLSTL 605
           LQKSPR +SGK SR LSKK  DEG  IT+D+LHKL+P N+SAWNMKRLMN+VRHG LSTL
Sbjct: 546 LQKSPRVKSGKFSRPLSKKS-DEGNVITMDNLHKLNPNNISAWNMKRLMNMVRHGALSTL 605

Query: 606 DEQIKDTAHEDESTTEIKSEYEAKVAAKKIFLNVARNGSKYIYLEDLMRFMEKDEASKTM 665
           DEQI D +++D++ T+I+SEYEAK AAKKIF NVAR G +YIY +DLMRFM +DEA+KTM
Sbjct: 606 DEQILDNSNDDDNATQIRSEYEAKAAAKKIFHNVARRGCRYIYPDDLMRFMREDEAAKTM 665

Query: 666 GLFEGACESRKISKSSLKNWVVNAFRERRALALTLNDTKT-------------------- 725
            LFEGA E+ KISKS+LKNWVVNAFRERRALALTLNDTKT                    
Sbjct: 666 NLFEGASEAGKISKSALKNWVVNAFRERRALALTLNDTKTAVNKLHRMLNFIVGIIILVI 725

Query: 726 -------AVNKLHRM-----------------TVFESIIFLFVMHPFDVGDRCEIDGVQM 785
                  A  K                     T+FE+IIFLFVMHPFDVGDRCEIDGVQM
Sbjct: 726 WLLILELATTKFLLFVSSQVVVVAFIFGNTCKTIFEAIIFLFVMHPFDVGDRCEIDGVQM 785

Query: 786 IVEEMNILTTIFLRYDNQKIIFPNSVLATKAIHNFYRSPDMGDGIEFCLHISTPPEKIAI 845
           +VEEMNILTTIFLR+DNQK+I PN+VLATKAI+N+YRSPDMGD IEFC+HISTP EKI++
Sbjct: 786 VVEEMNILTTIFLRFDNQKVIIPNNVLATKAIYNYYRSPDMGDAIEFCVHISTPVEKISL 845

Query: 846 MRQRIISYIEGKKEHWCPAPMIVLKDVEELNRMRIAIWLTHRMNHQDMGERWTRRALLVE 878
           ++ RI SYI+ KKEHW P+P+IV +D ++LN +R+AIW THRMN QDMGER+ RR+LL+E
Sbjct: 846 IKHRIQSYIDNKKEHWYPSPLIVYRDYDQLNMVRLAIWPTHRMNFQDMGERFVRRSLLLE 905

BLAST of Cucsa.119450.2 vs. TrEMBL
Match: G7IDC2_MEDTR (Mechanosensitive ion channel family protein OS=Medicago truncatula GN=MTR_1g113850 PE=4 SV=1)

HSP 1 Score: 1046.2 bits (2704), Expect = 2.2e-302
Identity = 572/939 (60.92%), Postives = 683/939 (72.74%), Query Frame = 1

Query: 6   KSFKSHPSY--KYVRKLSGADATQDHEHLPILSDHQHSIMAVSDSSDRKEVIVKVDEADA 65
           KSFKS+ S   K+ RK S   A  DHE LPIL D QHS  +   + D   V+VK+++   
Sbjct: 6   KSFKSYASQNNKHSRKFS---ADSDHEQLPILHDRQHSSRSFPMAGD---VVVKINDDGG 65

Query: 66  STLRATDLVNGSGTIWRESSYDFWNDS-DNRRNYGEGGAKTTDDFEFRQHRKDVEDPPSK 125
              +A +       IWRESSYDFWND+ DN R+         + F+FR   K +EDPPS+
Sbjct: 66  EPSQANN------KIWRESSYDFWNDTGDNVRD---------ESFDFRNKAK-LEDPPSQ 125

Query: 126 LIGQFLHKQKASGEMSLDMDMEMLELPQDKT----PLSTVAESPMRRSSRELKVSFESIS 185
           LIG+FLHKQ+ASG+M LDMD+EM EL  +       L+ V ESP     RELKVSFE  +
Sbjct: 126 LIGKFLHKQRASGDMLLDMDLEMEELQNEGNGADGKLTPVEESPTV-IQRELKVSFEEPA 185

Query: 186 EISE------NDSMRRRH-RDSP-LDEEHRGQQPRQCDRRAHGSN-GEDDGAAEVLRCSS 245
             S       ND +RRRH +DSP + E  R  QP   DRR   S  G   G  EVLRCSS
Sbjct: 186 SNSNGIDAVVNDPVRRRHSKDSPSMGEYARPPQPPNHDRRRSPSPAGHGGGDCEVLRCSS 245

Query: 246 NSFFQRDVSFQRKSSLLRAKTKSRLLDPPEHQDRRSGRVPKSGQVRSGLISKALDEEDDD 305
           N+ F+R++S QRKS+L++ KTKSRL+DPP+  DRRSGRV KS Q+ SG+I +  D+++DD
Sbjct: 246 NASFERNLSMQRKSTLMKTKTKSRLMDPPDEPDRRSGRVAKSSQLLSGMIGRKGDDDEDD 305

Query: 306 PFLEEDLPDEYKKANLGVLTLLQWASLILIIAALVCTLTIRYWRRKKLWKLEVWKWEVMI 365
           PF+EED PDEYKK +  +  LL+W SLILII A V T  +   R KKLW+L++WKWEVMI
Sbjct: 306 PFMEEDFPDEYKKTHFSLWILLEWLSLILIIGASVTTFCVPLLREKKLWQLKLWKWEVMI 365

Query: 366 LVLICGRLVSGWGIRVIVFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLFD 425
           LVLICGRLVS W IR+ VF IERNFLLRKRVLYFVYGVRKAVQNC+WLGLVLIAW+FLFD
Sbjct: 366 LVLICGRLVSDWVIRIAVFCIERNFLLRKRVLYFVYGVRKAVQNCVWLGLVLIAWHFLFD 425

Query: 426 DKVQREVKSNALEYVTKVLVCLLVSTLVWLVKTLMVKVLASSFHVSTYFDRIQDALFNQY 485
            +VQRE  S+ L+YVTKVLVC LV TLVWL+KTL+VKVLASSFHVSTYFDRIQ++LFNQ+
Sbjct: 426 KRVQRETNSDVLQYVTKVLVCFLVGTLVWLLKTLVVKVLASSFHVSTYFDRIQESLFNQF 485

Query: 486 VIETLSGPPLIEIQKNEEEEERLAEEVIKLQNAGATIPPDLKATAFSTAQKGGRVIGSGG 545
           VIETLSGPPL+EI+K EEEEERLA+EV KLQNAG +IP DL+A+AF   + G   + SG 
Sbjct: 486 VIETLSGPPLVEIRKAEEEEERLADEVQKLQNAGVSIPADLRASAFPNIKSGR--LRSGM 545

Query: 546 LQKSPRGRSGKLSRTLSKKGGDEGI-------TIDHLHKLSPKNVSAWNMKRLMNIVRHG 605
           LQKSP  +SGK S  LSKK  D GI       TIDHLHKL+P NVSAWNMKRLMN+VRHG
Sbjct: 546 LQKSPVVKSGKFSMPLSKKSDDNGIGNGGGGITIDHLHKLNPNNVSAWNMKRLMNMVRHG 605

Query: 606 TLSTLDEQIKDTAHEDESTTEIKSEYEAKVAAKKIFLNVARNGSKYIYLEDLMRFMEKDE 665
            L+TLDEQI D++ +DE  T+I+SE EAK AAKKIF NVAR G ++IY EDLMRFM +DE
Sbjct: 606 ALTTLDEQILDSSADDEHATQIRSENEAKAAAKKIFQNVARRGCRFIYPEDLMRFMREDE 665

Query: 666 ASKTMGLFEGACESRKISKSSLKNWVVNAFRERRALALTLNDTK---------------- 725
           A KT+ LFEGA +S KISKS+LKNWVVNAFRERRALALTLNDTK                
Sbjct: 666 AIKTINLFEGASDSGKISKSALKNWVVNAFRERRALALTLNDTKTAVNKLHRMLNFLVAI 725

Query: 726 -----------TAVNKLHRM-----------------TVFESIIFLFVMHPFDVGDRCEI 785
                       A  K                     TVFE+IIFLFVMHPFDVGDRCEI
Sbjct: 726 IILVIWLLILEIATTKFLLFVSSQLVLVAFIFGNTCKTVFEAIIFLFVMHPFDVGDRCEI 785

Query: 786 DGVQMIVEEMNILTTIFLRYDNQKIIFPNSVLATKAIHNFYRSPDMGDGIEFCLHISTPP 845
           D  QM+VEEMNILTT+FLR+DNQKI  PNSVLATKAIHNFYRSPDMGD +EFC+H++TPP
Sbjct: 786 DATQMVVEEMNILTTVFLRFDNQKITIPNSVLATKAIHNFYRSPDMGDSVEFCIHVATPP 845

Query: 846 EKIAIMRQRIISYIEGKKEHWCPAPMIVLKDVEELNRMRIAIWLTHRMNHQDMGERWTRR 878
           EKI++M+ RI ++I+ KKEHW P+P IVLKD E+LN +++AIW THRMN QDMGER+ RR
Sbjct: 846 EKISLMKHRIHNFIDNKKEHWYPSPFIVLKDHEQLNMVKVAIWPTHRMNFQDMGERYIRR 905

BLAST of Cucsa.119450.2 vs. TAIR10
Match: AT2G17000.1 (AT2G17000.1 Mechanosensitive ion channel family protein)

HSP 1 Score: 767.7 bits (1981), Expect = 7.8e-222
Identity = 437/920 (47.50%), Postives = 585/920 (63.59%), Query Frame = 1

Query: 6   KSFKSHPSYKYVRKLSGADATQDHEHLPILSDHQHSIMAVSDSSDRKEVIVKVDEADAST 65
           K FKSH SYK +  +S  D                     ++ + +K+ +  +D+ D + 
Sbjct: 5   KPFKSHSSYKQI--ISTGDQ--------------------NEKTKKKKKLANLDDGDIAK 64

Query: 66  LRATDLVNGSGTIWRESSYDFWNDSDNRRNYGEGGAKTTDDFEFRQHRKDVEDPPSKLIG 125
            ++      SG+ +  +SY FW D     +Y + G+     F+F Q+R+           
Sbjct: 65  TQS------SGSSFDGNSYKFWQDIATD-DYTKSGS-----FDFPQYRE----------- 124

Query: 126 QFLHKQKASGEMSLDMDMEMLELPQDKTPLSTVAESPMRRSSRELKVSFESISEISENDS 185
                     E++LD++ E  E          V+ +     S+E +V F+  S  + N S
Sbjct: 125 ----------EITLDVNEETEETED-------VSNNNNLSGSKETRVFFKINSSGTNNMS 184

Query: 186 MRRRHRDSPLDEEHRGQQPRQCDRRAHGSNGEDDGAAEVLRCSSNSFFQRDVSFQRKSSL 245
              R   S                R +     +D    V+RCSS           RK+ L
Sbjct: 185 GSVRSCTSSTSFS-------SATMRLNLEQQLEDEGEVVVRCSS----------VRKTEL 244

Query: 246 L-RAKTKSRLLDPPEHQDRR-SGRVPKSGQVRSGLISKALD--EEDDDPFLEEDLPDEYK 305
           + RAK +SRL+DPP+ ++++ S  +  S Q+RSGL+ +  D  EE+DD   EED+P EY+
Sbjct: 245 VSRAKARSRLIDPPQEEEQQYSSWIGTSDQLRSGLLGRHSDDIEEEDDSSAEEDVPVEYR 304

Query: 306 KANLGVLTLLQWASLILIIAALVCTLTIRYWRRKKLWKLEVWKWEVMILVLICGRLVSGW 365
           K  +  +TLLQW SLI ++ ALV +L +  WR   LW L +WKWEV++LVLICGRLVSG 
Sbjct: 305 KLKMDAITLLQWMSLIALVVALVLSLGLHTWRNATLWSLHLWKWEVVLLVLICGRLVSGC 364

Query: 366 GIRVIVFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLFDDKVQREVKSNAL 425
           GIR+IVFFIERNFLLRKRVLYFVYGV+ AVQNCLWLGLVL+AW+FLFD KV++E +S+ L
Sbjct: 365 GIRIIVFFIERNFLLRKRVLYFVYGVKTAVQNCLWLGLVLLAWHFLFDKKVEKETQSDVL 424

Query: 426 EYVTKVLVCLLVSTLVWLVKTLMVKVLASSFHVSTYFDRIQDALFNQYVIETLSGPPLIE 485
             ++K+LVC L+ST++WL+KTL+VKVLASSFHVSTYFDRIQ+ALF+ Y+IETLSGPP++E
Sbjct: 425 LLMSKILVCFLLSTVLWLIKTLVVKVLASSFHVSTYFDRIQEALFHHYLIETLSGPPMLE 484

Query: 486 IQKNEEEEERLAEEVIKLQNAGATIPPDLKATAFSTAQKGGRVIGSGGLQKSPRGRSGKL 545
           + + EEEE+R  +E+ K+Q  GA + P+L + AF   + G  +             + K 
Sbjct: 485 LSRIEEEEDRTQDEIYKMQKGGADLSPELCSAAFPQEKSGSTM-------------NMKF 544

Query: 546 SRTLSKKGGDEGITIDHLHKLSPKNVSAWNMKRLMNIVRHGTLSTLDEQIKDTAHEDEST 605
           S  + K G D GIT+D LHK++ KNVSAWNMKRLM IVR+ +LSTLDEQ      EDEST
Sbjct: 545 SPIIPKTGSDNGITMDDLHKMNQKNVSAWNMKRLMKIVRNVSLSTLDEQALQNTCEDEST 604

Query: 606 TEIKSEYEAKVAAKKIFLNVARNGSKYIYLEDLMRFMEKDEASKTMGLFEGACESRKISK 665
            +I+SE EAK AA+KIF NVA+ G+K+IYLEDLMRF+  DEA KTM LFEGA  ++KI+K
Sbjct: 605 RQIRSEKEAKAAARKIFKNVAQPGTKHIYLEDLMRFLRVDEAMKTMCLFEGALVTKKITK 664

Query: 666 SSLKNWVVNAFRERRALALTLND---------------------------TKTAVNKL-- 725
           S+LKNW+VNAFRERRALALTLND                            + A +K   
Sbjct: 665 SALKNWLVNAFRERRALALTLNDTKTAVNKLHHMISFLTAIVIIVIWLILLEIATSKYLL 724

Query: 726 ---------------HRMTVFESIIFLFVMHPFDVGDRCEIDGVQMIVEEMNILTTIFLR 785
                             TVFESIIFLF++HP+DVGDR  ID V+M+VEEMNILTT+FLR
Sbjct: 725 FLTSQVVLLAFMFGNSLKTVFESIIFLFIIHPYDVGDRLLIDTVEMVVEEMNILTTVFLR 784

Query: 786 YDNQKIIFPNSVLATKAIHNFYRSPDMGDGIEFCLHISTPPEKIAIMRQRIISYIEGKKE 845
            DN KI++PN +L  KAIHN+ RSPDMGD +  C+HI+TPPEKIA ++QRI SYI+ K E
Sbjct: 785 ADNLKIVYPNILLWQKAIHNYNRSPDMGDEVTCCVHITTPPEKIAAIKQRISSYIDSKPE 832

Query: 846 HWCPAPMIVLKDVEELNRMRIAIWLTHRMNHQDMGERWTRRALLVEELVKIFQELDLQYR 878
           +W P   +++KDVE+LN +RIAIWL H++NHQ+MGER+TRRALL+EE++KI  ELD+QYR
Sbjct: 845 YWYPKADVIVKDVEDLNIVRIAIWLCHKINHQNMGERFTRRALLIEEVIKILLELDIQYR 832

BLAST of Cucsa.119450.2 vs. TAIR10
Match: AT3G14810.1 (AT3G14810.1 mechanosensitive channel of small conductance-like 5)

HSP 1 Score: 636.3 bits (1640), Expect = 2.7e-182
Identity = 360/700 (51.43%), Postives = 467/700 (66.71%), Query Frame = 1

Query: 43  MAVSDSSDRKEVIVKVDEADASTLRATDLVNGS--GTIWRESSYDFWNDSDNRRNYGEGG 102
           MA  DS+DR++ IV ++  ++  + AT   + +  G IW+ESSYDFW D +  +N  +G 
Sbjct: 1   MAAVDSTDRRDFIVNINGQESGAVGATGSSSNAEGGNIWKESSYDFW-DGEKGKNDKKGD 60

Query: 103 AKTTD--DFEFRQHRK------DVEDPPSKLIGQFLHKQKASG-EMSLDMDMEMLELPQD 162
            +  D   F FRQ  +      ++ DPPSKLIGQFLHKQ+ASG E+SLD+++ M EL  +
Sbjct: 61  DEDEDGGSFHFRQRGERRHSSAELSDPPSKLIGQFLHKQRASGDEISLDVELNMAELQSN 120

Query: 163 KTPLSTVAESPMRRSSRELKVSFESISEISENDSMRRRHRDSPL----DEEHRGQQPRQC 222
             P    A +  RR    +  S   +    + D++RRR   + L    DEE R +     
Sbjct: 121 TPPRPATASNTPRRGLTTISESSSPVKTKVKADAVRRRQNRTSLGGSSDEEGRNRDE--- 180

Query: 223 DRRAHGSNGEDDGAAEVLRCSSNSFFQRDVSFQRKSSLLRAKTKSRLLDPP-------EH 282
                         AEVL+C S           +K  L R KTKSRL DPP       + 
Sbjct: 181 --------------AEVLKCGS-----------KKPMLSRNKTKSRLQDPPTPTHPAIDK 240

Query: 283 QDRRSGRVPKSGQVRSGLISKAL-----------DEEDDDPFLEEDLPDEYKKANLGVLT 342
            + +SGR  +SG  +SG + K+            +EE++DPFL+EDLP+E+K+  L    
Sbjct: 241 TEMKSGR--RSGIFKSGFLGKSPKAGTPGRNGFEEEEEEDPFLDEDLPEEFKRDKLSFWV 300

Query: 343 LLQWASLILIIAALVCTLTIRYWRRKKLWKLEVWKWEVMILVLICGRLVSGWGIRVIVFF 402
            L+W SL+LI+ +LVC+LTI   +RK  WKL++WKWEV +LVLICGRLVS W +R+IVF 
Sbjct: 301 FLEWISLVLIVTSLVCSLTIHNLQRKTWWKLDLWKWEVTVLVLICGRLVSSWIVRIIVFL 360

Query: 403 IERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLFDDKVQREVKSNALEYVTKVLV 462
           +E+NF  RKRVLYFVYGVRK+VQNCLWLGLVL+AW+FLFD KV+RE +S AL YVT+VLV
Sbjct: 361 VEKNFTWRKRVLYFVYGVRKSVQNCLWLGLVLLAWHFLFDKKVERETRSTALRYVTRVLV 420

Query: 463 CLLVSTLVWLVKTLMVKVLASSFHVSTYFDRIQDALFNQYVIETLSGPPLIEIQKNEEEE 522
           CLLV+ ++WLVKT++VKVLASSFH+STYFDRIQ++LF QYVIETLSGPPL+EIQ+ EEEE
Sbjct: 421 CLLVALIIWLVKTILVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPLMEIQRMEEEE 480

Query: 523 ERLAEEVIKLQN-AGATIPPDLKATAFSTAQKGGRVIGSGGLQKSPRGRSGKLSRTLSKK 582
           +++AE+V  L+  AGA +PP LKAT  S  + G          KSP      L+R  SK+
Sbjct: 481 QQVAEDVKSLEKLAGAKLPPALKATVKSFMKVG----------KSP-----GLNRIGSKR 540

Query: 583 GGD-EGITIDHLHKLSPKNVSAWNMKRLMNIVRHGTLSTLDEQIKDTAHEDESTTEIKSE 642
           G D EGI ID L +++ KNVSAWNMKRLMNI+  G +STLD+ ++DT  EDE  T I+SE
Sbjct: 541 GEDGEGIRIDQLKRMNTKNVSAWNMKRLMNIILKGAISTLDQNMQDTTQEDEDATHIRSE 600

Query: 643 YEAKVAAKKIFLNVARNGSKYIYLEDLMRFMEKDEASKTMGLFEGACESRKISKSSLKNW 702
           YEAK AA+KIF NV   GS+YIYLED +RF+ ++EA + M LFEGA ES KISKS LKNW
Sbjct: 601 YEAKCAARKIFHNVTEPGSRYIYLEDFLRFLCEEEAERAMALFEGASESDKISKSCLKNW 654

Query: 703 VVNAFRERRALALTLNDTKTAVNKLHR-MTVFESIIFLFV 707
           VV AFRERRALALTLNDTKTAV++LHR + V   II + +
Sbjct: 661 VVKAFRERRALALTLNDTKTAVDRLHRIINVVIGIIIIII 654

HSP 2 Score: 283.1 bits (723), Expect = 5.8e-76
Identity = 127/175 (72.57%), Postives = 157/175 (89.71%), Query Frame = 1

Query: 696 TVFESIIFLFVMHPFDVGDRCEIDGVQMIVEEMNILTTIFLRYDNQKIIFPNSVLATKAI 755
           T+FE+IIFLFVMHPFDVGDRCEIDGVQ++VEEMNILTT+FLRYDNQKII+PNSVL TK I
Sbjct: 687 TIFEAIIFLFVMHPFDVGDRCEIDGVQLVVEEMNILTTVFLRYDNQKIIYPNSVLGTKPI 746

Query: 756 HNFYRSPDMGDGIEFCLHISTPPEKIAIMRQRIISYIEGKKEHWCPAPMIVLKDVEELNR 815
            N+YRSPDMGD +EFC+HI+TPPEKI  ++QRI+SY++ KK++W PAPMIV   +++LN 
Sbjct: 747 ANYYRSPDMGDAVEFCVHIATPPEKITAIKQRILSYVDNKKDYWYPAPMIVFLSMDDLNS 806

Query: 816 MRIAIWLTHRMNHQDMGERWTRRALLVEELVKIFQELDLQYRLLPLDINVRSLPP 871
           ++IA+WLTHRMNHQDMGER+ RR LL+EE+ K  +ELD++YRL PL+INVRSLPP
Sbjct: 807 VKIAVWLTHRMNHQDMGERYIRRGLLLEEVGKTCRELDIEYRLYPLNINVRSLPP 861

BLAST of Cucsa.119450.2 vs. TAIR10
Match: AT1G53470.1 (AT1G53470.1 mechanosensitive channel of small conductance-like 4)

HSP 1 Score: 620.2 bits (1598), Expect = 2.0e-177
Identity = 368/755 (48.74%), Postives = 486/755 (64.37%), Query Frame = 1

Query: 43  MAVSDSSDRKEVIVKVDEADASTLRATDLVNG-SGTIWRESSYDFW-NDSDNRRNYGEGG 102
           MAV  +  R++ +V++D  D          NG S   WRESS +FW ND  ++   GE  
Sbjct: 1   MAVDSTDQRRDFVVRIDGED----------NGDSEKFWRESSINFWHNDKSSKPPGGEED 60

Query: 103 AKTTDDFEFRQHRKDVEDPPSKLIGQFLHKQKASG-EMSLDMDMEMLELPQDKTP-LSTV 162
             + D       + +  DPPSKLI QFL+KQKASG E+SLDM+  M EL ++  P LS+ 
Sbjct: 61  DGSFDFMRRSSEKSEEPDPPSKLINQFLNKQKASGDEISLDMEANMPELQKNTVPPLSST 120

Query: 163 AESPMRRSSRELKVSFESISEISENDSMRRRHRDSPLDEEHR-GQQPRQCDRRAHGSNGE 222
           A S    S+  +     +       D++RRR     L    + G      + R  GS   
Sbjct: 121 AVSG---SASPVTAPVTASYRNGTGDAIRRRQNRVTLSPSVKDGDSSEDEENRVDGS--- 180

Query: 223 DDGAAEVLRCSSNSFFQRDVSFQRKSSLLRAKTKSRLLDPPE--HQDRRSGRVPKSGQVR 282
                EV++C+SN       S  R  +L++ KT+SRL+DPP   + D  SGR P+SG + 
Sbjct: 181 -----EVVKCTSNR------STMRTKTLMKMKTRSRLMDPPTPTYPDMVSGRTPRSGNLN 240

Query: 283 SGLISKALD------------EEDDDPFLEEDLPDEYKKANLGVLTLLQWASLILIIAAL 342
            G   +               EE++DPF EEDLP+  +K  + V  +++W  LILIIA+L
Sbjct: 241 PGFSGRNTKPGTPNQGGSKDLEEEEDPFSEEDLPEGLRKEKICVWVIIEWIFLILIIASL 300

Query: 343 VCTLTIRYWRRKKLWKLEVWKWEVMILVLICGRLVSGWGIRVIVFFIERNFLLRKRVLYF 402
           +C+L I Y R K LW L +WKWEVM+LVLICGRLVS W +++ V+F+E NFL RK+VLYF
Sbjct: 301 ICSLVIPYLRGKTLWDLALWKWEVMVLVLICGRLVSSWIVKLFVYFVESNFLWRKKVLYF 360

Query: 403 VYGVRKAVQNCLWLGLVLIAWNFLFDDKVQREVKSNALEYVTKVLVCLLVSTLVWLVKTL 462
           VYG+RK VQNCLWLGLVLIAW+FLFD KV+RE++S  L+YVTKVL+CLLV+ ++WL+KTL
Sbjct: 361 VYGIRKPVQNCLWLGLVLIAWHFLFDKKVEREMRSTVLKYVTKVLICLLVAVIIWLIKTL 420

Query: 463 MVKVLASSFHVSTYFDRIQDALFNQYVIETLSGPPLIEIQKNEEEEERLAEEVIKLQNAG 522
           +VKVLASSFH+STYFDRIQ++LF QYVIETLSGPP IEI     EEE++A +V   +  G
Sbjct: 421 LVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPRIEI---HIEEEKVANDVKTFEIVG 480

Query: 523 ATIPPDLKATAFSTAQKGGRVIGSGGLQKSPR--GRSGKLSRTLSKK-GGDEGITIDHLH 582
             + P L   A S+  +    +GSG LQKSP   G+S  LSR+ SKK GG+EGI IDHL 
Sbjct: 481 RKLSP-LGPKAVSSPPQ--VTVGSGRLQKSPSRVGKSPVLSRSGSKKEGGEEGIRIDHLQ 540

Query: 583 KLSPKNVSAWNMKRLMNIVRHGTLSTLDEQIKD-TAHEDESTTEIKSEYEAKVAAKKIFL 642
           +++ KNVSAW MK+LMN+++ GTLSTLDEQI+D T  ED+  T+I+SE+EAK+AA+KIF 
Sbjct: 541 RMNTKNVSAWKMKKLMNVIKKGTLSTLDEQIQDTTTQEDDKATQIRSEFEAKLAARKIFQ 600

Query: 643 NVARNGSKYIYLEDLMRFMEKDEASKTMGLFEGACESRKISKSSLKNWVVNAFRERRALA 702
           NVA  GS+YIY+ED MRF+ +DE+ + M LFEGA E  KISKS LKNWVVNAFRERRALA
Sbjct: 601 NVAEPGSRYIYMEDFMRFLSEDESERAMDLFEGASECHKISKSCLKNWVVNAFRERRALA 660

Query: 703 LTLNDTKTAVNKLHRMT-VFESIIFLFVMHPFDVGDRCEIDGVQ----MIVEEMNILTTI 762
           LTLNDTKTAVN+LHR+  V  SI+ L +           I G+     ++V    +L  +
Sbjct: 661 LTLNDTKTAVNRLHRIVDVLVSIVILIIW--------LLILGIATTKFLLVISSQLLLVV 709

Query: 763 FLRYDNQKIIFPNSVLATKAIHNFYRSPDMGDGIE 770
           F+  ++ K IF  +V+    +H F    D+GD  E
Sbjct: 721 FVFGNSCKTIF-EAVIFVFVMHPF----DVGDRCE 709

HSP 2 Score: 264.6 bits (675), Expect = 2.1e-70
Identity = 120/185 (64.86%), Postives = 157/185 (84.86%), Query Frame = 1

Query: 696 TVFESIIFLFVMHPFDVGDRCEIDGVQMIVEEMNILTTIFLRYDNQKIIFPNSVLATKAI 755
           T+FE++IF+FVMHPFDVGDRCEIDGVQMIVEEMNILTT+FLR+DNQKI++PNS+L TK I
Sbjct: 688 TIFEAVIFVFVMHPFDVGDRCEIDGVQMIVEEMNILTTVFLRFDNQKIVYPNSLLGTKPI 747

Query: 756 HNFYRSPDMGDGIEFCLHISTPPEKIAIMRQRIISYIEGKKEHWCPAPMIVLKDVEELNR 815
            N+YRSPDM D IEF +HI+TPPEK   +RQRI+SY++ KK+HW P+PMIV +D+  LN 
Sbjct: 748 ANYYRSPDMQDAIEFFVHIATPPEKTTALRQRILSYVDNKKDHWHPSPMIVFRDMCGLNS 807

Query: 816 MRIAIWLTHRMNHQDMGERWTRRALLVEELVKIFQELDLQYRLLPLDINVRSLP---PVN 875
           ++IA+W TH+MNHQ+MGER+ RR  L+EE+ ++ +ELD++YRL PL+INV+SLP   P+ 
Sbjct: 808 VKIAMWPTHKMNHQNMGERYVRRGQLLEEIGRLCRELDIEYRLYPLNINVKSLPAATPIT 867

Query: 876 STNLP 878
           S  +P
Sbjct: 868 SDRIP 872

BLAST of Cucsa.119450.2 vs. TAIR10
Match: AT1G78610.1 (AT1G78610.1 mechanosensitive channel of small conductance-like 6)

HSP 1 Score: 595.1 bits (1533), Expect = 6.9e-170
Identity = 331/596 (55.54%), Postives = 416/596 (69.80%), Query Frame = 1

Query: 193 SPLDEEHRGQQPRQ-CDRRAHGSNGEDDGAAEVLRCSSNSFFQRDVSFQRKSS-LLRAKT 252
           +P+ E  R    R    RR   SN  ++   EV++CS N+        QR SS LL+ +T
Sbjct: 119 TPVSESPRVSTKRDPVGRRDSRSNTNNNDDGEVVKCSGNN-----APIQRSSSTLLKMRT 178

Query: 253 KSRLLDPPEHQ------DRRSGRVPKSGQVRSGLISKAL----DEEDDDPFLEEDLPDEY 312
           +SRL DPP  Q      D +SGR+PKSGQ++SG   K+     +EE+DDPF  EDLP+EY
Sbjct: 179 RSRLSDPPTPQLPPQTADMKSGRIPKSGQMKSGFFGKSPKTQGEEEEDDPFAAEDLPEEY 238

Query: 313 KKANLGVLTLLQWASLILIIAALVCTLTIRYWRRKKLWKLEVWKWEVMILVLICGRLVSG 372
           +K  L +  +L+W SLILIIA  VCTL I   R+KKLW+L++WKWE M+LVLICGRLVS 
Sbjct: 239 RKDKLSLWIVLEWLSLILIIAGFVCTLAIPSLRKKKLWELQLWKWESMVLVLICGRLVSS 298

Query: 373 WGIRVIVFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLFDDKVQREVKSNA 432
           W ++++VFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGLVL+AW+FLFD+KV +   + A
Sbjct: 299 WIVKIVVFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLLAWHFLFDEKVAKAANTKA 358

Query: 433 LEYVTKVLVCLLVSTLVWLVKTLMVKVLASSFHVSTYFDRIQDALFNQYVIETLSGPPLI 492
           L  VTK+ VCLLV  L+WLVKTL+VKVLASSFH+STYFDRIQ++LF QYVIETLSGPPLI
Sbjct: 359 LRVVTKIFVCLLVGFLLWLVKTLLVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPLI 418

Query: 493 EIQKNEEEEERLAEEVIKLQNAGATIPPDLKATAFSTAQKGGRVIGSGGLQKSP--RGRS 552
           EIQKNEEEEER++ EV K QN G                    V    G QKSP   G+S
Sbjct: 419 EIQKNEEEEERISVEVKKFQNPGG-------------------VEIQSGAQKSPMKTGKS 478

Query: 553 GKLSRTLSKKGG----DEGITIDHLHKLSPKNVSAWNMKRLMNIVRHGTLSTLDEQIKDT 612
             LS  LS  GG    ++GITID LHKL+PKNVSAW MKRLMNI+R+G+L+TLDEQ++D 
Sbjct: 479 PFLSHVLSNGGGGGGENKGITIDSLHKLNPKNVSAWKMKRLMNIIRNGSLTTLDEQLQDP 538

Query: 613 AHEDESTTEIKSEYEAKVAAKKIFLNVARNGSKYIYLEDLMRFMEKDEASKTMGLFEGAC 672
           + +D+   +I+SE+EAK+AA+KIF NVA+ GSK+IY  D+MRF+  DEA KT+ LFEGA 
Sbjct: 539 SLDDDKGNQIRSEFEAKLAARKIFHNVAKPGSKFIYANDIMRFLPDDEALKTLSLFEGAS 598

Query: 673 ESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMTVFESIIFLFVMHPFDVGD 732
           E+ +ISKSSLKNWVVNAFRERRALALTLNDTKTAVN+LH+M      I + V+    +G 
Sbjct: 599 ETNRISKSSLKNWVVNAFRERRALALTLNDTKTAVNRLHKMVNIVVGIIILVIWLIILG- 658

Query: 733 RCEIDGVQ-MIVEEMNILTTIFLRYDNQKIIFPNSVLATKAIHNFYRSPDMGDGIE 770
              I   + ++V    ++   F+  +  KI+F  S++    IH F    D+GD  E
Sbjct: 659 ---ITSTKFLVVMSSQVVVVAFIFGNMCKIVF-ESIIYLFVIHPF----DVGDRCE 681

HSP 2 Score: 288.5 bits (737), Expect = 1.4e-77
Identity = 134/181 (74.03%), Postives = 161/181 (88.95%), Query Frame = 1

Query: 697 VFESIIFLFVMHPFDVGDRCEIDGVQMIVEEMNILTTIFLRYDNQKIIFPNSVLATKAIH 756
           VFESII+LFV+HPFDVGDRCEIDGVQM+VEEMNILTT+FLR+DNQK+++PNS+L TK+I 
Sbjct: 661 VFESIIYLFVIHPFDVGDRCEIDGVQMVVEEMNILTTVFLRFDNQKVVYPNSLLWTKSIG 720

Query: 757 NFYRSPDMGDGIEFCLHISTPPEKIAIMRQRIISYIEGKKEHWCPAPMIVLKDVEELNRM 816
           N+YRSPDMGDGIEF +HI+TP EKI +++QRI SYIEGKK+HW PAPMIV KD+E LN +
Sbjct: 721 NYYRSPDMGDGIEFSIHITTPAEKIILIKQRITSYIEGKKDHWYPAPMIVFKDMESLNSV 780

Query: 817 RIAIWLTHRMNHQDMGERWTRRALLVEELVKIFQELDLQYRLLPLDINVRSLPPVNSTNL 876
           RIA+W THRMNHQDMGE+W RR+ LVEE+ KI +ELD++YRL PLDINVR+LP   ST L
Sbjct: 781 RIAVWPTHRMNHQDMGEKWARRSQLVEEIAKICRELDIEYRLYPLDINVRNLP--TSTAL 839

Query: 877 P 878
           P
Sbjct: 841 P 839

HSP 3 Score: 99.4 bits (246), Expect = 1.2e-20
Identity = 67/158 (42.41%), Postives = 88/158 (55.70%), Query Frame = 1

Query: 43  MAVSDSSDRKEVIVKVDEADASTLRATDLVNGS--GTIWRESSYDFWNDSDNRRNYGEGG 102
           MAV D++DR+EVIVK+D  + +     + V+G   G IWR+ SYDFW D +   N G   
Sbjct: 1   MAV-DAADRREVIVKIDGENGNN----NGVSGETVGKIWRDGSYDFWTDGEGNLNKGHNA 60

Query: 103 AKTTDD--------------FEFRQHRKDVEDPPSKLIGQFLHKQKASGEMSLDMDMEML 162
           A    D              FEFR+     EDPP+KLIGQFLHKQ+ASGE+ LDMD+ M 
Sbjct: 61  AAVDSDRSAATTGEQQKDEGFEFRRG----EDPPTKLIGQFLHKQQASGEICLDMDLGMD 120

Query: 163 ELPQDKTPLSTVAESPMRRSSRELKVSFESISEISEND 185
           EL      L+ V+ESP   + R+     +S S  + ND
Sbjct: 121 EL--QSRGLTPVSESPRVSTKRDPVGRRDSRSNTNNND 147

BLAST of Cucsa.119450.2 vs. TAIR10
Match: AT2G17010.1 (AT2G17010.1 Mechanosensitive ion channel family protein)

HSP 1 Score: 424.5 bits (1090), Expect = 1.6e-118
Identity = 213/371 (57.41%), Postives = 279/371 (75.20%), Query Frame = 1

Query: 551 DEGITIDHLHKLSPKNVSAWNMKRLMNIVRHGTLSTLDEQIKDTAHEDESTTEIKSEYEA 610
           D+ +  +   +++ KN+SAWNMKRLM IVR+ +L+TLDEQ+ ++ +EDEST +I+SE EA
Sbjct: 400 DKKVQRETRSRMNHKNISAWNMKRLMKIVRNVSLTTLDEQMLESTYEDESTRQIRSEKEA 459

Query: 611 KVAAKKIFLNVARNGSKYIYLEDLMRFMEKDEASKTMGLFEGACESRKISKSSLKNWVVN 670
           K AA+KIF NV + G+KYIYLEDLMRF+ +DEA KTMGLFEGA E+++ISKS+LKNW+VN
Sbjct: 460 KAAARKIFKNVEQRGAKYIYLEDLMRFLREDEAMKTMGLFEGAPENKRISKSALKNWLVN 519

Query: 671 AFRERRALALTLNDTKT---------------------------AVNKLHRM-------- 730
           AFRERRALALTLNDTKT                           A +K+           
Sbjct: 520 AFRERRALALTLNDTKTAVNKLHHMINIVTAIVIVVIWLVLLEIASSKVLLFVSSQVVLL 579

Query: 731 ---------TVFESIIFLFVMHPFDVGDRCEIDGVQMIVEEMNILTTIFLRYDNQKIIFP 790
                    TVFESIIFLF++HP+DVGDRCEID VQ++VEEMNILTT+FLRYDN KI++P
Sbjct: 580 AFIFGNTVKTVFESIIFLFIVHPYDVGDRCEIDSVQLVVEEMNILTTVFLRYDNLKIMYP 639

Query: 791 NSVLATKAIHNFYRSPDMGDGIEFCLHISTPPEKIAIMRQRIISYIEGKKEHWCPAPMIV 850
           NS+L  K+I+N+YRSPDMGD IEFC+HI+TP EKI++++QRI +YI+ K E+W P   I+
Sbjct: 640 NSLLWQKSINNYYRSPDMGDAIEFCVHITTPLEKISVIKQRISNYIDNKPEYWYPQAKII 699

Query: 851 LKDVEELNRMRIAIWLTHRMNHQDMGERWTRRALLVEELVKIFQELDLQYRLLPLDINVR 878
           +KD+E+L+ +R+AIW  HR+NHQDM ERWTRRA+LVEE++KI  ELD+Q+R  PLDINVR
Sbjct: 700 VKDLEDLHIVRLAIWPCHRINHQDMAERWTRRAVLVEEVIKILLELDIQHRFYPLDINVR 759

HSP 2 Score: 308.5 bits (789), Expect = 1.3e-83
Identity = 203/500 (40.60%), Postives = 277/500 (55.40%), Query Frame = 1

Query: 1   MDFSLKSFKSHPSYKYVRKLSGADATQDHEHLPILSDHQ--HSIMAVSDSSDRKEVIVKV 60
           MDF   SFKSH SYK +R   G  +    EHLPIL DH   HS M V D         K 
Sbjct: 1   MDFR-NSFKSHSSYKQIRS-PGDQSEPSPEHLPILHDHHPDHSGMVVDDQ--------KP 60

Query: 61  DEADASTLRATDLVNG-SGTIWRESSYDFWNDSDNRRNYGEGGAKTTD------------ 120
           D   +S      L +G +  + R++SY FW D+    +      +T+D            
Sbjct: 61  DSTRSS------LDDGRNAPVERDASYKFWQDNTTGTSTDHTAVRTSDKDPIAISRKGDR 120

Query: 121 ---DFEFRQHRKDVEDPPSKLI-GQFLHKQ---KASGEMSLDMDMEMLELPQDKTPLSTV 180
               F+F   +  V++ P+K++ G+ +++Q   + + E++LD+D E  ++     P  T 
Sbjct: 121 LSGSFDFVHGKLPVDESPTKMVAGEPVNRQWRGRNNEEITLDVDQENDDVSHQTMPTPTS 180

Query: 181 AESPMRRSSRELKVSFE-----------------SISEISENDSMRRRHRDSPLDEEHRG 240
                  +SRE++VSF                  S S  S + +MR       L EE   
Sbjct: 181 TARTSFDASREMRVSFNVRRAGGAFVAGSVPSSSSHSSSSSSATMRTNQDQPQLQEE--- 240

Query: 241 QQPRQCDRRAHGSNGEDDGAAEVLRCSSNSFFQRDVSFQRKSSLL-RAKTKSRLLDPP-E 300
                                EV+RC+SN      +SFQRKS L+ R KT+SRL DPP E
Sbjct: 241 ---------------------EVVRCTSN------MSFQRKSELISRVKTRSRLQDPPRE 300

Query: 301 HQDRRSGRVPKSGQVRSGLISKALDEEDDDPFLEEDLPDEYKKANLGVLTLLQWASLILI 360
            +   SG   +SGQ++SGL++    +E+DDP  EED+PDEYK+  L  +TLLQW SL+ I
Sbjct: 301 EETPYSGW--RSGQLKSGLLADI--DEEDDPLAEEDVPDEYKRGKLDAITLLQWLSLVAI 360

Query: 361 IAALVCTLTIRYWRRKKLWKLEVWKWEVMILVLICGRLVSGWGIRVIVFFIERNFLLRKR 420
           IAAL C+L+I+ W++ ++W L +WKWEV +LVLICGRLVSGWGIR++VFFIERNFLLRKR
Sbjct: 361 IAALACSLSIQSWKKVRVWNLHLWKWEVFLLVLICGRLVSGWGIRIVVFFIERNFLLRKR 420

Query: 421 VLYFVYGVRKAVQNCLWLGLVLIAWNFLFDDKVQREVKSNALEYVTKVLVCLLVSTLVWL 460
           VLYFVYGVR+AVQNCLWLGLVL+AW+FLFD KVQRE +S       K +    +  L+ +
Sbjct: 421 VLYFVYGVRRAVQNCLWLGLVLLAWHFLFDKKVQRETRSRMNH---KNISAWNMKRLMKI 447

BLAST of Cucsa.119450.2 vs. NCBI nr
Match: gi|449444568|ref|XP_004140046.1| (PREDICTED: mechanosensitive ion channel protein 6-like [Cucumis sativus])

HSP 1 Score: 1711.8 bits (4432), Expect = 0.0e+00
Identity = 872/923 (94.47%), Postives = 873/923 (94.58%), Query Frame = 1

Query: 1   MDFSLKSFKSHPSYKYVRKLSGADATQDHEHLPILSDHQHSIMAVSDSSDRKEVIVKVDE 60
           MDFSLKSFKSHPSYKYVRKLSGADATQDHEHLPILSDHQHSIMAVSDSSDRKEVIVKVDE
Sbjct: 1   MDFSLKSFKSHPSYKYVRKLSGADATQDHEHLPILSDHQHSIMAVSDSSDRKEVIVKVDE 60

Query: 61  ADASTLRATDLVNGSGTIWRESSYDFWNDSDNRRNYGEGGAKTTDDFEFRQHRKDVEDPP 120
           ADASTLRATDLVNGSGTIWRESSYDFWNDSDNRRNYGEGGAKTTDDFEFRQHRKDVEDPP
Sbjct: 61  ADASTLRATDLVNGSGTIWRESSYDFWNDSDNRRNYGEGGAKTTDDFEFRQHRKDVEDPP 120

Query: 121 SKLIGQFLHKQKASGEMSLDMDMEMLELPQDKTPLSTVAESPMRRSSRELKVSFESISEI 180
           SKLIGQFLHKQKASGEMSLDMDMEMLELPQDKTPLSTVAESPMRRSSRELKVSFESISEI
Sbjct: 121 SKLIGQFLHKQKASGEMSLDMDMEMLELPQDKTPLSTVAESPMRRSSRELKVSFESISEI 180

Query: 181 SENDSMRRRHRDSPLDEEHRGQQPRQCDRRAHGSNGEDDGAAEVLRCSSNSFFQRDVSFQ 240
           SENDSMRRRHRDSPLDEEHRGQQPRQCDRRAHGSNGEDDGAAEVLRCSSNSFFQRDVSFQ
Sbjct: 181 SENDSMRRRHRDSPLDEEHRGQQPRQCDRRAHGSNGEDDGAAEVLRCSSNSFFQRDVSFQ 240

Query: 241 RKSSLLRAKTKSRLLDPPEHQDRRSGRVPKSGQVRSGLISKALDEEDDDPFLEEDLPDEY 300
           RKSSLLRAKTKSRLLDPPEHQDRRSGRVPKSGQVRSGLISKALDEEDDDPFLEEDLPDEY
Sbjct: 241 RKSSLLRAKTKSRLLDPPEHQDRRSGRVPKSGQVRSGLISKALDEEDDDPFLEEDLPDEY 300

Query: 301 KKANLGVLTLLQWASLILIIAALVCTLTIRYWRRKKLWKLEVWKWEVMILVLICGRLVSG 360
           KKANLGVLTLLQWASLILIIAALVCTLTIRYWRRKKLWKLEVWKWEVMILVLICGRLVSG
Sbjct: 301 KKANLGVLTLLQWASLILIIAALVCTLTIRYWRRKKLWKLEVWKWEVMILVLICGRLVSG 360

Query: 361 WGIRVIVFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLFDDKVQREVKSNA 420
           WGIRVIVFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLFDDKVQREVKSNA
Sbjct: 361 WGIRVIVFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLFDDKVQREVKSNA 420

Query: 421 LEYVTKVLVCLLVSTLVWLVKTLMVKVLASSFHVSTYFDRIQDALFNQYVIETLSGPPLI 480
           LEYVTKVLVCLLVSTLVWLVKTLMVKVLASSFHVSTYFDRIQDALFNQYVIETLSGPPLI
Sbjct: 421 LEYVTKVLVCLLVSTLVWLVKTLMVKVLASSFHVSTYFDRIQDALFNQYVIETLSGPPLI 480

Query: 481 EIQKNEEEEERLAEEVIKLQNAGATIPPDLKATAFSTAQKGGRVIGSGGLQKSPRGRSGK 540
           EIQKNEEEEERLAEEVIKLQNAGATIPPDLKATAFSTAQKGGRVIGSGGLQKSPRGRSGK
Sbjct: 481 EIQKNEEEEERLAEEVIKLQNAGATIPPDLKATAFSTAQKGGRVIGSGGLQKSPRGRSGK 540

Query: 541 LSRTLSKKGGDEGITIDHLHKLSPKNVSAWNMKRLMNIVRHGTLSTLDEQIKDTAHEDES 600
           LSRTLSKKGGDEGITIDHLHKLSPKNVSAWNMKRLMNIVRHGTLSTLDEQIKDTAHEDES
Sbjct: 541 LSRTLSKKGGDEGITIDHLHKLSPKNVSAWNMKRLMNIVRHGTLSTLDEQIKDTAHEDES 600

Query: 601 TTEIKSEYEAKVAAKKIFLNVARNGSKYIYLEDLMRFMEKDEASKTMGLFEGACESRKIS 660
           TTEIKSEYEAKVAAKKIFLNVARNGSKYIYLEDLMRFMEKDEASKTMGLFEGACESRKIS
Sbjct: 601 TTEIKSEYEAKVAAKKIFLNVARNGSKYIYLEDLMRFMEKDEASKTMGLFEGACESRKIS 660

Query: 661 KSSLKNWVVNAFRERRALALTLNDTK---------------------------TAVNKLH 720
           KSSLKNWVVNAFRERRALALTLNDTK                            A +K  
Sbjct: 661 KSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVIILVIWLLILGIATSKFL 720

Query: 721 RM-----------------TVFESIIFLFVMHPFDVGDRCEIDGVQMIVEEMNILTTIFL 780
                              TVFESIIFLFVMHPFDVGDRCEIDGVQMIVEEMNILTTIFL
Sbjct: 721 LFVTSQLVLVAFVFGNTCKTVFESIIFLFVMHPFDVGDRCEIDGVQMIVEEMNILTTIFL 780

Query: 781 RYDNQKIIFPNSVLATKAIHNFYRSPDMGDGIEFCLHISTPPEKIAIMRQRIISYIEGKK 840
           RYDNQKIIFPNSVLATKAIHNFYRSPDMGDGIEFCLHISTPPEKIAIMRQRIISYIEGKK
Sbjct: 781 RYDNQKIIFPNSVLATKAIHNFYRSPDMGDGIEFCLHISTPPEKIAIMRQRIISYIEGKK 840

Query: 841 EHWCPAPMIVLKDVEELNRMRIAIWLTHRMNHQDMGERWTRRALLVEELVKIFQELDLQY 880
           EHWCPAPMIVLKDVEELNRMRIAIWLTHRMNHQDMGERWTRRALLVEELVKIFQELDLQY
Sbjct: 841 EHWCPAPMIVLKDVEELNRMRIAIWLTHRMNHQDMGERWTRRALLVEELVKIFQELDLQY 900

BLAST of Cucsa.119450.2 vs. NCBI nr
Match: gi|659095000|ref|XP_008448342.1| (PREDICTED: mechanosensitive ion channel protein 6-like [Cucumis melo])

HSP 1 Score: 1685.6 bits (4364), Expect = 0.0e+00
Identity = 856/923 (92.74%), Postives = 866/923 (93.82%), Query Frame = 1

Query: 1   MDFSLKSFKSHPSYKYVRKLSGADATQDHEHLPILSDHQHSIMAVSDSSDRKEVIVKVDE 60
           MDFSLKSFKSHPSYKYVRKLSGADATQD EHLPILSDHQHSIMAVSD+SDRKEVIVKVDE
Sbjct: 1   MDFSLKSFKSHPSYKYVRKLSGADATQDQEHLPILSDHQHSIMAVSDASDRKEVIVKVDE 60

Query: 61  ADASTLRATDLVNGSGTIWRESSYDFWNDSDNRRNYGEGGAKTTDDFEFRQHRKDVEDPP 120
           ADAS+LRATDLVNGSG IWRESSYDFWNDSDNRRN GEGGA+TT+DFEFRQHRKDVEDPP
Sbjct: 61  ADASSLRATDLVNGSGRIWRESSYDFWNDSDNRRNNGEGGARTTEDFEFRQHRKDVEDPP 120

Query: 121 SKLIGQFLHKQKASGEMSLDMDMEMLELPQDKTPLSTVAESPMRRSSRELKVSFESISEI 180
           SKLIGQFLHKQKASGEMSLDMDMEMLELPQDKTPLSTVAESPMRRSSRELKVSFESISEI
Sbjct: 121 SKLIGQFLHKQKASGEMSLDMDMEMLELPQDKTPLSTVAESPMRRSSRELKVSFESISEI 180

Query: 181 SENDSMRRRHRDSPLDEEHRGQQPRQCDRRAHGSNGEDDGAAEVLRCSSNSFFQRDVSFQ 240
           SENDSMRRRHRDSPLDEEHRGQQPRQCDRRAHGSNGEDDGAAEVLRCSSNS FQRDVSFQ
Sbjct: 181 SENDSMRRRHRDSPLDEEHRGQQPRQCDRRAHGSNGEDDGAAEVLRCSSNSSFQRDVSFQ 240

Query: 241 RKSSLLRAKTKSRLLDPPEHQDRRSGRVPKSGQVRSGLISKALDEEDDDPFLEEDLPDEY 300
           RKSSLLRAKTKSRLLDPPEHQDRRSGRVPKSGQVRSGLISK LDEEDDDPFLEEDLPDEY
Sbjct: 241 RKSSLLRAKTKSRLLDPPEHQDRRSGRVPKSGQVRSGLISKVLDEEDDDPFLEEDLPDEY 300

Query: 301 KKANLGVLTLLQWASLILIIAALVCTLTIRYWRRKKLWKLEVWKWEVMILVLICGRLVSG 360
           KKANLGVLTLLQWASLILIIAALVCTLTIRYWRRKKLWKLEVWKWEVMILVLICGRLVSG
Sbjct: 301 KKANLGVLTLLQWASLILIIAALVCTLTIRYWRRKKLWKLEVWKWEVMILVLICGRLVSG 360

Query: 361 WGIRVIVFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLFDDKVQREVKSNA 420
           WGIRVIVFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLFDDKVQREVKSNA
Sbjct: 361 WGIRVIVFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLFDDKVQREVKSNA 420

Query: 421 LEYVTKVLVCLLVSTLVWLVKTLMVKVLASSFHVSTYFDRIQDALFNQYVIETLSGPPLI 480
           LEYVTKVLVCLL+STLVWLVKTLMVKVLASSFHVSTYFDRIQDALFNQYVIETLSGPPLI
Sbjct: 421 LEYVTKVLVCLLISTLVWLVKTLMVKVLASSFHVSTYFDRIQDALFNQYVIETLSGPPLI 480

Query: 481 EIQKNEEEEERLAEEVIKLQNAGATIPPDLKATAFSTAQKGGRVIGSGGLQKSPRGRSGK 540
           EIQKNEEEEERLAEEV+KLQNAGATIPPDLKATAFSTAQKGGRVIGSGGLQKSPRGRSGK
Sbjct: 481 EIQKNEEEEERLAEEVMKLQNAGATIPPDLKATAFSTAQKGGRVIGSGGLQKSPRGRSGK 540

Query: 541 LSRTLSKKGGDEGITIDHLHKLSPKNVSAWNMKRLMNIVRHGTLSTLDEQIKDTAHEDES 600
           LSRTLSKKGGDEGITIDHLHKLSPKNVSAWNMKRLMNIVRHGTLSTLDEQIKDTAHEDES
Sbjct: 541 LSRTLSKKGGDEGITIDHLHKLSPKNVSAWNMKRLMNIVRHGTLSTLDEQIKDTAHEDES 600

Query: 601 TTEIKSEYEAKVAAKKIFLNVARNGSKYIYLEDLMRFMEKDEASKTMGLFEGACESRKIS 660
           TT+IKSEYEAKVAAKKIFLNVARNGSKYIYLEDLMRFMEKDEASKTMGLFEGACESRKIS
Sbjct: 601 TTQIKSEYEAKVAAKKIFLNVARNGSKYIYLEDLMRFMEKDEASKTMGLFEGACESRKIS 660

Query: 661 KSSLKNWVVNAFRERRALALTLNDTK---------------------------TAVNKLH 720
           KSSLKNWVVNAFRERRALALTLNDTK                            A +K  
Sbjct: 661 KSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVIILVIWLLILGIATSKFL 720

Query: 721 RM-----------------TVFESIIFLFVMHPFDVGDRCEIDGVQMIVEEMNILTTIFL 780
                              TVFESIIFLFVMHPFDVGDRCEIDGVQMIVEEMNILTTIFL
Sbjct: 721 LFVTSQLVLVAFVFGNTCKTVFESIIFLFVMHPFDVGDRCEIDGVQMIVEEMNILTTIFL 780

Query: 781 RYDNQKIIFPNSVLATKAIHNFYRSPDMGDGIEFCLHISTPPEKIAIMRQRIISYIEGKK 840
           RYDNQKIIFPNSVLATKAIHNFYRSPDMGDG+EFCLHISTPPEKIAIMRQRI+SYIEGKK
Sbjct: 781 RYDNQKIIFPNSVLATKAIHNFYRSPDMGDGVEFCLHISTPPEKIAIMRQRIVSYIEGKK 840

Query: 841 EHWCPAPMIVLKDVEELNRMRIAIWLTHRMNHQDMGERWTRRALLVEELVKIFQELDLQY 880
           EHWCPAPMIVLKDVEELNRMRIAIWLTHRMNHQDMGERWTRRALLVEELVKIFQELDLQY
Sbjct: 841 EHWCPAPMIVLKDVEELNRMRIAIWLTHRMNHQDMGERWTRRALLVEELVKIFQELDLQY 900

BLAST of Cucsa.119450.2 vs. NCBI nr
Match: gi|1009150892|ref|XP_015893266.1| (PREDICTED: mechanosensitive ion channel protein 6-like [Ziziphus jujuba])

HSP 1 Score: 1133.2 bits (2930), Expect = 0.0e+00
Identity = 606/968 (62.60%), Postives = 712/968 (73.55%), Query Frame = 1

Query: 1   MDFSLK-SFKSHPSYKYVRKLSGADATQDHEH-----LPILSDH----------QH---- 60
           MDFSLK SFKSH SYK + K+ G      H H     LPIL D           QH    
Sbjct: 1   MDFSLKKSFKSHGSYKQMLKIGGGIGNDHHSHDDPEGLPILFDQDVAVAEHHPQQHLDSM 60

Query: 61  -SIMAVSDSSDRKEVIVKVDEADASTLRAT--DLVNGSGTIWRESSYDFWNDSDNRRNYG 120
            S     DS++R+EVIVK+D+ D+ST              IWR SSY+FW + DN    G
Sbjct: 61  SSAAVAGDSNNRREVIVKIDDGDSSTSSRNMEQQQQQQSKIWRGSSYEFWKEDDNNVRDG 120

Query: 121 EGGAKTTDDFEFRQHRKDV------EDPPSKLIGQFLHKQKASGEMSLDMDMEMLELPQD 180
                  DDF F Q  +        +DPPSKLIGQFLHKQKASG+MSLDMD+EM EL  +
Sbjct: 121 N-----KDDFRFVQRGQSSSASASPDDPPSKLIGQFLHKQKASGDMSLDMDLEMDELRHE 180

Query: 181 KTPLSTVAESPMRRSS----RELKVSFESISEISENDSMRRRHRDSPLDEEHRGQQPRQC 240
           +  L  +AESP +RSS    +ELKVSF+  +  + ND +  R     L E  R +     
Sbjct: 181 RN-LPPLAESPAKRSSFGQSKELKVSFQPTTTAASNDDVEIR-----LSESVRRRNKDST 240

Query: 241 DRRAHGSNGEDDGAAEVLRCSSNSFFQRDVSFQRKSSLLRAK-TKSRLLDPPEHQDRRSG 300
           +  + G +G  +G  EV+RC+SN+ F+R+VSFQ KSSLLR K TKSRL+DPPE  + R+ 
Sbjct: 241 EEDSSGGSGRGNGDDEVVRCTSNAAFEREVSFQSKSSLLRLKKTKSRLIDPPEEPENRAS 300

Query: 301 RVPKSGQV------------RSGLISK-ALDEEDDDPFLEEDLPDEYKKANLGVLTLLQW 360
           RVPKSGQV            RSG++    LD++DDDPF E+DLPD+YKKANL  LTLLQW
Sbjct: 301 RVPKSGQVMKSGQMLKSGQLRSGMLGNWPLDDDDDDPFWEDDLPDDYKKANLSALTLLQW 360

Query: 361 ASLILIIAALVCTLTIRYWRRKKLWKLEVWKWEVMILVLICGRLVSGWGIRVIVFFIERN 420
            SLI+II    CTL+I + RRK  WKL++WKWEV++LVLICGRL SGWG+R++VFFIERN
Sbjct: 361 LSLIVIIGVFACTLSIPFLRRKNWWKLKLWKWEVLVLVLICGRLFSGWGVRIVVFFIERN 420

Query: 421 FLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLFDDKVQREVKSNALEYVTKVLVCLLV 480
           FLLRKRVLYFVYG+RKAVQNCLWLGLVL+AW+FLFD+KV+RE KS+ L+YVTKVLVCLLV
Sbjct: 421 FLLRKRVLYFVYGLRKAVQNCLWLGLVLLAWHFLFDEKVERETKSDKLKYVTKVLVCLLV 480

Query: 481 STLVWLVKTLMVKVLASSFHVSTYFDRIQDALFNQYVIETLSGPPLIEIQKNEEEEERLA 540
            TLVWLVKTL+VKVLASSFHVS YFDRIQ++LFNQYVIETLSGPPLIE+++ EEE+ERL 
Sbjct: 481 GTLVWLVKTLIVKVLASSFHVSKYFDRIQESLFNQYVIETLSGPPLIEMKRTEEEDERLV 540

Query: 541 EEVIKLQNAGATIPPDLKATAFSTAQKGGRVIGSGGLQKSPRGRSGKLSRTLSKKGGDEG 600
           +EV KLQNAGATIPPDLK  AF TA K GRVIGSGGLQKSPR RS K S+ LSKK  D+G
Sbjct: 541 DEVRKLQNAGATIPPDLKNAAFPTA-KSGRVIGSGGLQKSPR-RSNKFSQPLSKKQ-DDG 600

Query: 601 ITIDHLHKLSPKNVSAWNMKRLMNIVRHGTLSTLDEQIKDTAHEDESTTEIKSEYEAKVA 660
           ITIDHLHKL+PKNVSAWNMKRLMNIVRHG+L+TLDEQI+D+  +DE  T+IKSE EAK A
Sbjct: 601 ITIDHLHKLNPKNVSAWNMKRLMNIVRHGSLTTLDEQIQDSTRDDEKATQIKSEVEAKAA 660

Query: 661 AKKIFLNVARNGSKYIYLEDLMRFMEKDEASKTMGLFEGACESRKISKSSLKNWVVNAFR 720
           AKKIF NVAR+GSK+IYL DL RF+  DEA KTM LFEGA ES KISK+SLKNWVVNAFR
Sbjct: 661 AKKIFQNVARHGSKHIYLRDLTRFLRDDEALKTMSLFEGASESGKISKTSLKNWVVNAFR 720

Query: 721 ERRALALTLNDTKTAVNKLH---------------------------------------- 780
           ERRALALTLNDTKTAVNKLH                                        
Sbjct: 721 ERRALALTLNDTKTAVNKLHHMVKIIIIFVIGVIWLLILGIATTKFLLFVSSQLVLVAFI 780

Query: 781 ----RMTVFESIIFLFVMHPFDVGDRCEIDGVQMIVEEMNILTTIFLRYDNQKIIFPNSV 840
                 TVFE+IIFLFV+HPFDVGDRCEIDGVQM+VEEMNILTT+FLRYDN KI+FPNSV
Sbjct: 781 FGNTCKTVFEAIIFLFVVHPFDVGDRCEIDGVQMVVEEMNILTTVFLRYDNTKIVFPNSV 840

Query: 841 LATKAIHNFYRSPDMGDGIEFCLHISTPPEKIAIMRQRIISYIEGKKEHWCPAPMIVLKD 878
           L+TK I+N+YRSPDMGD +EFC+H+STP E+IA ++ RI SYIE KKEHWC  PMIV+KD
Sbjct: 841 LSTKPINNYYRSPDMGDAVEFCIHVSTPAEEIAAIKHRITSYIENKKEHWCTQPMIVMKD 900

BLAST of Cucsa.119450.2 vs. NCBI nr
Match: gi|703062965|ref|XP_010086848.1| (Mechanosensitive ion channel protein 8 [Morus notabilis])

HSP 1 Score: 1114.8 bits (2882), Expect = 0.0e+00
Identity = 613/966 (63.46%), Postives = 717/966 (74.22%), Query Frame = 1

Query: 1   MDFSLK-SFKSHPSYKYVRKLS--GADATQDHEHLPILSDHQ----HSIM---AVSD--- 60
           MDFSLK SFKSH SYK  RK+S  G +    HE LPILSDH     H +     ++D   
Sbjct: 1   MDFSLKKSFKSHGSYKNSRKISAGGGNPDNSHEELPILSDHDFDDNHHVQHYRRLNDNGK 60

Query: 61  ----SSDRKEVIVKVDEADASTLRATDLVNGSGTIWRESSYDFWNDSDNRRNYGEGGAKT 120
               S+ R EVIVK+DE       A+D    +   WRE+SY  W ++ N R+ G  G   
Sbjct: 61  MGGASTGRNEVIVKIDEGGNPPAAASDQSRNAENFWRENSYGAWKENANVRDGGGSG--- 120

Query: 121 TDDFEFRQHRKDVEDPPSKLIGQFLHKQKASGEMSLDMDMEMLELPQD-----KTPLSTV 180
            + F F Q     EDPPSKLI QFLH+QKASG++SLDMD+EM EL +      +  L+ V
Sbjct: 121 -ESFHFAQ---TTEDPPSKLIRQFLHRQKASGDISLDMDLEMEELRRSAGSGGELDLTPV 180

Query: 181 AESPMR-RSSRELKVSFESI-----SEISENDSMRRRHRDSPLDEEHRGQQPRQCDRRAH 240
           +ESP   RSSRELKVS         SEIS+ND++RRR +DSP D +         +RR  
Sbjct: 181 SESPQNSRSSRELKVSLHPSPSDVGSEISQNDAVRRRFKDSPNDNDE------SLERR-- 240

Query: 241 GSNGEDDGAAEVLRCSSNSFFQRDVSFQRKSSLLRAKTKSRLLDPPEHQDRRSGRVPKSG 300
               E DG  EV+RC+SN+ FQR++SFQR+SS LR KT+SRL DPPE  D RSGR+PKSG
Sbjct: 241 ----ERDGE-EVVRCTSNASFQREISFQRRSSFLRVKTRSRLQDPPEEPDMRSGRIPKSG 300

Query: 301 QV------------RSGLISKALDEEDDDPFLEEDLPDEYKKANLGVLTLLQWASLILII 360
           Q+            +SGL+ K  DEE+DDPF +EDLPDE+KKANL  LTLLQWASLILI+
Sbjct: 301 QIPRSGPIPRSGPMKSGLLGK--DEEEDDPFSDEDLPDEFKKANLSALTLLQWASLILIV 360

Query: 361 AALVCTLTIRYWRRKKLWKLEVWKWEVMILVLICGRLVSGWGIRVIVFFIERNFLLRKRV 420
               CTL+I Y R K L KL++WKWEV+ILVLICGRLVSGWGIR+ VFF ERNFLLRKRV
Sbjct: 361 GLFACTLSIHYLRHKNLLKLKLWKWEVLILVLICGRLVSGWGIRIAVFFFERNFLLRKRV 420

Query: 421 LYFVYGVRKAVQNCLWLGLVLIAWNFLFDDKVQREVKSNALEYVTKVLVCLLVSTLVWLV 480
           LYFVYGVRKAVQNCLWLGLVLIAW+FLFD +V+RE +S +L+YVTKVL+C LV TL+WLV
Sbjct: 421 LYFVYGVRKAVQNCLWLGLVLIAWHFLFDKRVERETRSESLKYVTKVLICFLVGTLLWLV 480

Query: 481 KTLMVKVLASSFHVSTYFDRIQDALFNQYVIETLSGPPLIEIQKNEEEEERLAEEVIKLQ 540
           KTL+VKVLASSFHVSTYFDRIQD+LFNQ+VIETLSGPPLIEI K EEEEERLA+EV KLQ
Sbjct: 481 KTLIVKVLASSFHVSTYFDRIQDSLFNQFVIETLSGPPLIEIYKTEEEEERLADEVEKLQ 540

Query: 541 NAGATIPPDLKATAFSTA-QKGGRVIGSGGLQKSPRGRSGKLSRTLSKKGGDEGITIDHL 600
           NAGATIPPDL   AFS+A  +  RVIGSG L ++   +S KLSR+LSK G DEGITIDHL
Sbjct: 541 NAGATIPPDL---AFSSALPRTSRVIGSGRLSRT--WKSSKLSRSLSKIG-DEGITIDHL 600

Query: 601 HKLSPKNVSAWNMKRLMNIVRHGTLSTLDEQIKDTAHEDESTTEIKSEYEAKVAAKKIFL 660
           HKL+PKNVSAWNMKRLM +VRHGTL+TLDEQI D+ HEDES T+I+SE EAK AAKKIF 
Sbjct: 601 HKLNPKNVSAWNMKRLMRMVRHGTLTTLDEQIVDSTHEDESATQIRSEVEAKAAAKKIFQ 660

Query: 661 NVARNGSKYIYLEDLMRFMEKDEASKTMGLFEGACESRKISKSSLKNWVVNAFRERRALA 720
           NVAR GSK+I LEDLMRFM +DEA KTM LFEGA ES++ISKSSLKNWVVNAFRERRALA
Sbjct: 661 NVARRGSKFICLEDLMRFMREDEAVKTMSLFEGASESQRISKSSLKNWVVNAFRERRALA 720

Query: 721 LTLNDTKTA---------------------------VNKLHRM----------------- 780
           LTLNDTKTA                            +K                     
Sbjct: 721 LTLNDTKTAVNKLHRIVNVIVAIVIGVIWLLILGIATSKFLLFISSQLVLVAFIFGNTCK 780

Query: 781 TVFESIIFLFVMHPFDVGDRCEIDGVQMIVEEMNILTTIFLRYDNQKIIFPNSVLATKAI 840
           TVFE+I+FLFVMHPFDVGDRCEIDGVQM+VEEMNILTT+FLRYDN KIIFPNSVLATKAI
Sbjct: 781 TVFEAIVFLFVMHPFDVGDRCEIDGVQMVVEEMNILTTVFLRYDNAKIIFPNSVLATKAI 840

Query: 841 HNFYRSPDMGDGIEFCLHISTPPEKIAIMRQRIISYIEGKKEHWCPAPMIVLKDVEELNR 878
            NFYRSPDMGDGIEF +H++TP +KIA MRQRI +Y+E KKEHW PAPM+++KD++ELNR
Sbjct: 841 DNFYRSPDMGDGIEFSIHVATPADKIAAMRQRITNYVENKKEHWYPAPMVIMKDLDELNR 900

BLAST of Cucsa.119450.2 vs. NCBI nr
Match: gi|659094533|ref|XP_008448113.1| (PREDICTED: mechanosensitive ion channel protein 6-like [Cucumis melo])

HSP 1 Score: 1105.9 bits (2859), Expect = 0.0e+00
Identity = 585/941 (62.17%), Postives = 707/941 (75.13%), Query Frame = 1

Query: 1   MDFSLKSFKSHPSYKYVRKLS--GADATQDHEHLPILSDHQ---HSIMAVSDSSDRKEVI 60
           MD   KSFK + S+K+ RK+S  G  +  +HE LPIL +HQ   H ++  SD SDR EVI
Sbjct: 1   MDTLKKSFKGNVSFKHTRKISAGGVSSEINHEELPILLNHQSTDHHLVNDSDPSDRTEVI 60

Query: 61  VKVDEADASTL-RATDLV-NGSGTIWRESSYDFWNDSDNRRNYGEG-----GAKTTDD-- 120
           +K+D+  +S + R+ D V N  G +WRES YDFWN+ +     GE      GA+ +D   
Sbjct: 61  LKIDDGGSSAVSRSLDSVANNGGKVWRESRYDFWNNDET--GIGESASRVRGARMSDSGD 120

Query: 121 -----FEFRQHRKDVEDPPSKLIGQFLHKQKASGEMSLDMDMEMLELPQDKTPLSTVAES 180
                F+F Q    +EDPP+KLIG FLHKQK  GE +LDMD+EM EL  ++  +  +AES
Sbjct: 121 DGNEGFQFVQTGYGMEDPPTKLIGDFLHKQKIRGETTLDMDLEMEELKPNRI-IPPLAES 180

Query: 181 PMRRSSRELKVSFESIS-EISEND-SMRRRHRDS-PLDEEHRGQQP--RQCDRRAHGS-- 240
           P+ ++S++LKVSF+  S EIS ND SMRRR+RDS  L EE +G+QP  +Q      GS  
Sbjct: 181 PLSQTSKDLKVSFQQDSTEISSNDQSMRRRYRDSHDLKEEFKGEQPPWQQSHHERLGSPT 240

Query: 241 --NGEDDGAAEVLRCSSNSFFQRDVSFQRKSSLLRAKTKSRLLDPPEHQDRRSGRVPKSG 300
               +++  AE +RC+SN  F  ++SFQRKS+LLRAKTKSRL DPP   DR SG +PKSG
Sbjct: 241 ISGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLTDPPAEPDRLSGLIPKSG 300

Query: 301 QVRSGLISKALDEEDDDPFLEEDLPDEYKKANLGVLTLLQWASLILIIAALVCTLTIRYW 360
           Q+RSG + K ++++DDDPFLE+DLPD++K+ N   LT+LQW SLILI AA +CTL++ Y 
Sbjct: 301 QLRSGFLGK-IEDDDDDPFLEDDLPDDFKRGNFTALTVLQWFSLILITAAFICTLSVPYL 360

Query: 361 RRKKLWKLEVWKWEVMILVLICGRLVSGWGIRVIVFFIERNFLLRKRVLYFVYGVRKAVQ 420
           R K LW+L++WKWEVMIL+LICGRLVSGWGIR+ VFFIERNFLLRKRVLYFVYGVRK VQ
Sbjct: 361 REKSLWELDIWKWEVMILILICGRLVSGWGIRIAVFFIERNFLLRKRVLYFVYGVRKPVQ 420

Query: 421 NCLWLGLVLIAWNFLFDDKVQREVKSNALEYVTKVLVCLLVSTLVWLVKTLMVKVLASSF 480
           NCLWLGLVLIAW+ LF+ +V+++  ++ L YV++VLVCLL+STL+WLVKTLMVKVLASSF
Sbjct: 421 NCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKVLASSF 480

Query: 481 HVSTYFDRIQDALFNQYVIETLSGPPLIEIQKNEEEEERLAEEVIKLQNAGATIPPDLKA 540
           HVSTYFDRIQ++LFNQYVIETLSGPPL+EI+KNEEEEER+A+EV KLQNAG TIPPDLKA
Sbjct: 481 HVSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGLTIPPDLKA 540

Query: 541 TAFSTAQKGGRVIGSGGLQKSPRGRSGKLSRTLSKKGGDEGITIDHLHKLSPKNVSAWNM 600
           T  S   K GR I S    KS   +S K SR L+K G D GITIDHLHKLSPKNVSAWNM
Sbjct: 541 TFASI--KSGRAISSERTHKSFCAKSSKFSRALTKNGND-GITIDHLHKLSPKNVSAWNM 600

Query: 601 KRLMNIVRHGTLSTLDEQIKDTAHEDESTTEIKSEYEAKVAAKKIFLNVARNGSKYIYLE 660
           KRL+NIVR+G++STLDEQI+    +DESTTEIKSE EAK AAKKIF NVAR G KYIYL+
Sbjct: 601 KRLLNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVARRGYKYIYLD 660

Query: 661 DLMRFMEKDEASKTMGLFEGACESRKISKSSLKNWVVNAFRERRALALTLNDTKT----- 720
           DLMRFM +DE  KT  LFEGA E+R+ISKS LKNWVVN FRERRALALTLNDTKT     
Sbjct: 661 DLMRFMREDEVLKTTSLFEGAAENRRISKSVLKNWVVNVFRERRALALTLNDTKTAVDKL 720

Query: 721 ----------------------AVNKLHRM-----------------TVFESIIFLFVMH 780
                                 A +K                     T+FE+IIFLFVMH
Sbjct: 721 HHMVNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFVMH 780

Query: 781 PFDVGDRCEIDGVQMIVEEMNILTTIFLRYDNQKIIFPNSVLATKAIHNFYRSPDMGDGI 840
           PFDVGDRCEIDG QM+VEEMNILTT+FLRYDN KII PNSVLATK IHNFYRSPDMG+ +
Sbjct: 781 PFDVGDRCEIDGTQMVVEEMNILTTVFLRYDNLKIIIPNSVLATKLIHNFYRSPDMGESV 840

Query: 841 EFCLHISTPPEKIAIMRQRIISYIEGKKEHWCPAPMIVLKDVEELNRMRIAIWLTHRMNH 870
           EF +HI+TP EKI  M+QRIISYIEG KEHWCPAPMIV KD++ LN++++A+WL+HRMNH
Sbjct: 841 EFFVHIATPAEKITAMKQRIISYIEGNKEHWCPAPMIVFKDIDGLNKLKLAVWLSHRMNH 900