BLAST of Cucsa.119450 vs. Swiss-Prot
Match:
MSL8_ARATH (Mechanosensitive ion channel protein 8 OS=Arabidopsis thaliana GN=MSL8 PE=2 SV=2)
HSP 1 Score: 980.7 bits (2534), Expect = 1.1e-284
Identity = 524/962 (54.47%), Postives = 684/962 (71.10%), Query Frame = 1
Query: 1 MDFSLKSFKSHPSYKYVRKLSGADATQDHEHLPILSDHQ--HSIMAVSDSSDRKEVIVKV 60
MDF SFKSH SYK +R G + EHLPIL DH HS M V D K
Sbjct: 1 MDFR-NSFKSHSSYKQIRS-PGDQSEPSPEHLPILHDHHPDHSGMVVDDQ--------KP 60
Query: 61 DEADASTLRATDLVNG-SGTIWRESSYDFWNDSDNRRNYGEGGAKTTD------------ 120
D +S L +G + + R++SY FW D+ + +T+D
Sbjct: 61 DSTRSS------LDDGRNAPVERDASYKFWQDNTTGTSTDHTAVRTSDKDPIAISRKGDR 120
Query: 121 ---DFEFRQHRKDVEDPPSKLI-GQFLHKQ---KASGEMSLDMDMEMLELPQDKTPLSTV 180
F+F + V++ P+K++ G+ +++Q + + E++LD+D E ++ P T
Sbjct: 121 LSGSFDFVHGKLPVDESPTKMVAGEPVNRQWRGRNNEEITLDVDQENDDVSHQTMPTPTS 180
Query: 181 AESPMRRSSRELKVSFE-----------------SISEISENDSMRRRHRDSPLDEEHRG 240
+SRE++VSF S S S + +MR L EE
Sbjct: 181 TARTSFDASREMRVSFNVRRAGGAFVAGSVPSSSSHSSSSSSATMRTNQDQPQLQEE--- 240
Query: 241 QQPRQCDRRAHGSNGEDDGAAEVLRCSSNSFFQRDVSFQRKSSLL-RAKTKSRLLDPP-E 300
EV+RC+SN +SFQRKS L+ R KT+SRL DPP E
Sbjct: 241 ---------------------EVVRCTSN------MSFQRKSELISRVKTRSRLQDPPRE 300
Query: 301 HQDRRSGRVPKSGQVRSGLISKALDEEDDDPFLEEDLPDEYKKANLGVLTLLQWASLILI 360
+ SG +SGQ++SGL++ +E+DDP EED+PDEYK+ L +TLLQW SL+ I
Sbjct: 301 EETPYSGW--RSGQLKSGLLADI--DEEDDPLAEEDVPDEYKRGKLDAITLLQWLSLVAI 360
Query: 361 IAALVCTLTIRYWRRKKLWKLEVWKWEVMILVLICGRLVSGWGIRVIVFFIERNFLLRKR 420
IAAL C+L+I+ W++ ++W L +WKWEV +LVLICGRLVSGWGIR++VFFIERNFLLRKR
Sbjct: 361 IAALACSLSIQSWKKVRVWNLHLWKWEVFLLVLICGRLVSGWGIRIVVFFIERNFLLRKR 420
Query: 421 VLYFVYGVRKAVQNCLWLGLVLIAWNFLFDDKVQREVKSNALEYVTKVLVCLLVSTLVWL 480
VLYFVYGVR+AVQNCLWLGLVL+AW+FLFD KVQRE +S L YVTK+LVC L+ST++WL
Sbjct: 421 VLYFVYGVRRAVQNCLWLGLVLLAWHFLFDKKVQRETRSRFLPYVTKILVCFLLSTILWL 480
Query: 481 VKTLMVKVLASSFHVSTYFDRIQDALFNQYVIETLSGPPLIEIQKNEEEEERLAEEVIKL 540
+KTL+VKVLASSFHVSTYFDRIQ+ALFNQYVIETLSGPP+IE+ + EEEEER +E+ K+
Sbjct: 481 IKTLVVKVLASSFHVSTYFDRIQEALFNQYVIETLSGPPMIEMSRIEEEEERAQDEIFKM 540
Query: 541 QNAGATIPPDLKATAFSTAQKGGRVIGSGGLQKSPRGRSGKLSRTLSKKGGDEGITIDHL 600
QNAGA +PPDL A AF K GRV+ KLS + K D GI+++HL
Sbjct: 541 QNAGANLPPDLCAAAFPPG-KSGRVMNP------------KLSPIIPKSTTDNGISMEHL 600
Query: 601 HKLSPKNVSAWNMKRLMNIVRHGTLSTLDEQIKDTAHEDESTTEIKSEYEAKVAAKKIFL 660
H+++ KN+SAWNMKRLM IVR+ +L+TLDEQ+ ++ +EDEST +I+SE EAK AA+KIF
Sbjct: 601 HRMNHKNISAWNMKRLMKIVRNVSLTTLDEQMLESTYEDESTRQIRSEKEAKAAARKIFK 660
Query: 661 NVARNGSKYIYLEDLMRFMEKDEASKTMGLFEGACESRKISKSSLKNWVVNAFRERRALA 720
NV + G+KYIYLEDLMRF+ +DEA KTMGLFEGA E+++ISKS+LKNW+VNAFRERRALA
Sbjct: 661 NVEQRGAKYIYLEDLMRFLREDEAMKTMGLFEGAPENKRISKSALKNWLVNAFRERRALA 720
Query: 721 LTLNDTKTAVNKLHRMVNILVSVIILVIWLLILGIATSKFLLFVTSQLVLVAFVFGNTCK 780
LTLNDTKTAVNKLH M+NI+ +++I+VIWL++L IA+SK LLFV+SQ+VL+AF+FGNT K
Sbjct: 721 LTLNDTKTAVNKLHHMINIVTAIVIVVIWLVLLEIASSKVLLFVSSQVVLLAFIFGNTVK 780
Query: 781 TVFESIIFLFVMHPFDVGDRCEIDGVQMIVEEMNILTTIFLRYDNQKIIFPNSVLATKAI 840
TVFESIIFLF++HP+DVGDRCEID VQ++VEEMNILTT+FLRYDN KI++PNS+L K+I
Sbjct: 781 TVFESIIFLFIVHPYDVGDRCEIDSVQLVVEEMNILTTVFLRYDNLKIMYPNSLLWQKSI 840
Query: 841 HNFYRSPDMGDGIEFCLHISTPPEKIAIMRQRIISYIEGKKEHWCPAPMIVLKDVEELNR 900
+N+YRSPDMGD IEFC+HI+TP EKI++++QRI +YI+ K E+W P I++KD+E+L+
Sbjct: 841 NNYYRSPDMGDAIEFCVHITTPLEKISVIKQRISNYIDNKPEYWYPQAKIIVKDLEDLHI 899
Query: 901 MRIAIWLTHRMNHQDMGERWTRRALLVEELVKIFQELDLQYRLLPLDINVRSLPPVNSTN 922
+R+AIW HR+NHQDM ERWTRRA+LVEE++KI ELD+Q+R PLDINVR++P V S+
Sbjct: 901 VRLAIWPCHRINHQDMAERWTRRAVLVEEVIKILLELDIQHRFYPLDINVRTMPTVVSSR 899
BLAST of Cucsa.119450 vs. Swiss-Prot
Match:
MSL5_ARATH (Mechanosensitive ion channel protein 5 OS=Arabidopsis thaliana GN=MSL5 PE=2 SV=1)
HSP 1 Score: 979.5 bits (2531), Expect = 2.4e-284
Identity = 513/907 (56.56%), Postives = 661/907 (72.88%), Query Frame = 1
Query: 43 MAVSDSSDRKEVIVKVDEADASTLRATDLVNGS--GTIWRESSYDFWNDSDNRRNYGEGG 102
MA DS+DR++ IV ++ ++ + AT + + G IW+ESSYDFW D + +N +G
Sbjct: 1 MAAVDSTDRRDFIVNINGQESGAVGATGSSSNAEGGNIWKESSYDFW-DGEKGKNDKKGD 60
Query: 103 AKTTD--DFEFRQHRK------DVEDPPSKLIGQFLHKQKASG-EMSLDMDMEMLELPQD 162
+ D F FRQ + ++ DPPSKLIGQFLHKQ+ASG E+SLD+++ M EL +
Sbjct: 61 DEDEDGGSFHFRQRGERRHSSAELSDPPSKLIGQFLHKQRASGDEISLDVELNMAELQSN 120
Query: 163 KTPLSTVAESPMRRSSRELKVSFESISEISENDSMRRRHRDSPL----DEEHRGQQPRQC 222
P A + RR + S + + D++RRR + L DEE R +
Sbjct: 121 TPPRPATASNTPRRGLTTISESSSPVKTKVKADAVRRRQNRTSLGGSSDEEGRNRDE--- 180
Query: 223 DRRAHGSNGEDDGAAEVLRCSSNSFFQRDVSFQRKSSLLRAKTKSRLLDPP-------EH 282
AEVL+C S +K L R KTKSRL DPP +
Sbjct: 181 --------------AEVLKCGS-----------KKPMLSRNKTKSRLQDPPTPTHPAIDK 240
Query: 283 QDRRSGRVPKSGQVRSGLISKAL-----------DEEDDDPFLEEDLPDEYKKANLGVLT 342
+ +SGR +SG +SG + K+ +EE++DPFL+EDLP+E+K+ L
Sbjct: 241 TEMKSGR--RSGIFKSGFLGKSPKAGTPGRNGFEEEEEEDPFLDEDLPEEFKRDKLSFWV 300
Query: 343 LLQWASLILIIAALVCTLTIRYWRRKKLWKLEVWKWEVMILVLICGRLVSGWGIRVIVFF 402
L+W SL+LI+ +LVC+LTI +RK WKL++WKWEV +LVLICGRLVS W +R+IVF
Sbjct: 301 FLEWISLVLIVTSLVCSLTIHNLQRKTWWKLDLWKWEVTVLVLICGRLVSSWIVRIIVFL 360
Query: 403 IERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLFDDKVQREVKSNALEYVTKVLV 462
+E+NF RKRVLYFVYGVRK+VQNCLWLGLVL+AW+FLFD KV+RE +S AL YVT+VLV
Sbjct: 361 VEKNFTWRKRVLYFVYGVRKSVQNCLWLGLVLLAWHFLFDKKVERETRSTALRYVTRVLV 420
Query: 463 CLLVSTLVWLVKTLMVKVLASSFHVSTYFDRIQDALFNQYVIETLSGPPLIEIQKNEEEE 522
CLLV+ ++WLVKT++VKVLASSFH+STYFDRIQ++LF QYVIETLSGPPL+EIQ+ EEEE
Sbjct: 421 CLLVALIIWLVKTILVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPLMEIQRMEEEE 480
Query: 523 ERLAEEVIKLQN-AGATIPPDLKATAFSTAQKGGRVIGSGGLQKSPRGRSGKLSRTLSKK 582
+++AE+V L+ AGA +PP LKAT S + G KSP L+R SK+
Sbjct: 481 QQVAEDVKSLEKLAGAKLPPALKATVKSFMKVG----------KSPG-----LNRIGSKR 540
Query: 583 GGD-EGITIDHLHKLSPKNVSAWNMKRLMNIVRHGTLSTLDEQIKDTAHEDESTTEIKSE 642
G D EGI ID L +++ KNVSAWNMKRLMNI+ G +STLD+ ++DT EDE T I+SE
Sbjct: 541 GEDGEGIRIDQLKRMNTKNVSAWNMKRLMNIILKGAISTLDQNMQDTTQEDEDATHIRSE 600
Query: 643 YEAKVAAKKIFLNVARNGSKYIYLEDLMRFMEKDEASKTMGLFEGACESRKISKSSLKNW 702
YEAK AA+KIF NV GS+YIYLED +RF+ ++EA + M LFEGA ES KISKS LKNW
Sbjct: 601 YEAKCAARKIFHNVTEPGSRYIYLEDFLRFLCEEEAERAMALFEGASESDKISKSCLKNW 660
Query: 703 VVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVIILVIWLLILGIATSKFLLFVTSQL 762
VV AFRERRALALTLNDTKTAV++LHR++N+++ +II++IWLLILGIAT++FLL ++SQL
Sbjct: 661 VVKAFRERRALALTLNDTKTAVDRLHRIINVVIGIIIIIIWLLILGIATTRFLLVLSSQL 720
Query: 763 VLVAFVFGNTCKTVFESIIFLFVMHPFDVGDRCEIDGVQMIVEEMNILTTIFLRYDNQKI 822
+LVAFVFGN+CKT+FE+IIFLFVMHPFDVGDRCEIDGVQ++VEEMNILTT+FLRYDNQKI
Sbjct: 721 LLVAFVFGNSCKTIFEAIIFLFVMHPFDVGDRCEIDGVQLVVEEMNILTTVFLRYDNQKI 780
Query: 823 IFPNSVLATKAIHNFYRSPDMGDGIEFCLHISTPPEKIAIMRQRIISYIEGKKEHWCPAP 882
I+PNSVL TK I N+YRSPDMGD +EFC+HI+TPPEKI ++QRI+SY++ KK++W PAP
Sbjct: 781 IYPNSVLGTKPIANYYRSPDMGDAVEFCVHIATPPEKITAIKQRILSYVDNKKDYWYPAP 840
Query: 883 MIVLKDVEELNRMRIAIWLTHRMNHQDMGERWTRRALLVEELVKIFQELDLQYRLLPLDI 915
MIV +++LN ++IA+WLTHRMNHQDMGER+ RR LL+EE+ K +ELD++YRL PL+I
Sbjct: 841 MIVFLSMDDLNSVKIAVWLTHRMNHQDMGERYIRRGLLLEEVGKTCRELDIEYRLYPLNI 861
BLAST of Cucsa.119450 vs. Swiss-Prot
Match:
MSL4_ARATH (Mechanosensitive ion channel protein 4 OS=Arabidopsis thaliana GN=MSL4 PE=3 SV=1)
HSP 1 Score: 943.0 bits (2436), Expect = 2.5e-273
Identity = 500/905 (55.25%), Postives = 649/905 (71.71%), Query Frame = 1
Query: 43 MAVSDSSDRKEVIVKVDEADASTLRATDLVNG-SGTIWRESSYDFW-NDSDNRRNYGEGG 102
MAV + R++ +V++D D NG S WRESS +FW ND ++ GE
Sbjct: 1 MAVDSTDQRRDFVVRIDGED----------NGDSEKFWRESSINFWHNDKSSKPPGGEED 60
Query: 103 AKTTDDFEFRQHRKDVEDPPSKLIGQFLHKQKASG-EMSLDMDMEMLELPQDKTP-LSTV 162
+ D + + DPPSKLI QFL+KQKASG E+SLDM+ M EL ++ P LS+
Sbjct: 61 DGSFDFMRRSSEKSEEPDPPSKLINQFLNKQKASGDEISLDMEANMPELQKNTVPPLSST 120
Query: 163 AESPMRRSSRELKVSFESISEISENDSMRRRHRDSPLDEEHR-GQQPRQCDRRAHGSNGE 222
A S S+ + + D++RRR L + G + R GS
Sbjct: 121 AVSG---SASPVTAPVTASYRNGTGDAIRRRQNRVTLSPSVKDGDSSEDEENRVDGS--- 180
Query: 223 DDGAAEVLRCSSNSFFQRDVSFQRKSSLLRAKTKSRLLDPPE--HQDRRSGRVPKSGQVR 282
EV++C+SN S R +L++ KT+SRL+DPP + D SGR P+SG +
Sbjct: 181 -----EVVKCTSNR------STMRTKTLMKMKTRSRLMDPPTPTYPDMVSGRTPRSGNLN 240
Query: 283 SGLISKALD------------EEDDDPFLEEDLPDEYKKANLGVLTLLQWASLILIIAAL 342
G + EE++DPF EEDLP+ +K + V +++W LILIIA+L
Sbjct: 241 PGFSGRNTKPGTPNQGGSKDLEEEEDPFSEEDLPEGLRKEKICVWVIIEWIFLILIIASL 300
Query: 343 VCTLTIRYWRRKKLWKLEVWKWEVMILVLICGRLVSGWGIRVIVFFIERNFLLRKRVLYF 402
+C+L I Y R K LW L +WKWEVM+LVLICGRLVS W +++ V+F+E NFL RK+VLYF
Sbjct: 301 ICSLVIPYLRGKTLWDLALWKWEVMVLVLICGRLVSSWIVKLFVYFVESNFLWRKKVLYF 360
Query: 403 VYGVRKAVQNCLWLGLVLIAWNFLFDDKVQREVKSNALEYVTKVLVCLLVSTLVWLVKTL 462
VYG+RK VQNCLWLGLVLIAW+FLFD KV+RE++S L+YVTKVL+CLLV+ ++WL+KTL
Sbjct: 361 VYGIRKPVQNCLWLGLVLIAWHFLFDKKVEREMRSTVLKYVTKVLICLLVAVIIWLIKTL 420
Query: 463 MVKVLASSFHVSTYFDRIQDALFNQYVIETLSGPPLIEIQKNEEEEERLAEEVIKLQNAG 522
+VKVLASSFH+STYFDRIQ++LF QYVIETLSGPP IEI EEE++A +V + G
Sbjct: 421 LVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPRIEIHI---EEEKVANDVKTFEIVG 480
Query: 523 ATIPPDLKATAFSTAQKGGRVIGSGGLQKSPR--GRSGKLSRTLSKK-GGDEGITIDHLH 582
+ P S Q +GSG LQKSP G+S LSR+ SKK GG+EGI IDHL
Sbjct: 481 RKLSPLGPKAVSSPPQV---TVGSGRLQKSPSRVGKSPVLSRSGSKKEGGEEGIRIDHLQ 540
Query: 583 KLSPKNVSAWNMKRLMNIVRHGTLSTLDEQIKDTA-HEDESTTEIKSEYEAKVAAKKIFL 642
+++ KNVSAW MK+LMN+++ GTLSTLDEQI+DT ED+ T+I+SE+EAK+AA+KIF
Sbjct: 541 RMNTKNVSAWKMKKLMNVIKKGTLSTLDEQIQDTTTQEDDKATQIRSEFEAKLAARKIFQ 600
Query: 643 NVARNGSKYIYLEDLMRFMEKDEASKTMGLFEGACESRKISKSSLKNWVVNAFRERRALA 702
NVA GS+YIY+ED MRF+ +DE+ + M LFEGA E KISKS LKNWVVNAFRERRALA
Sbjct: 601 NVAEPGSRYIYMEDFMRFLSEDESERAMDLFEGASECHKISKSCLKNWVVNAFRERRALA 660
Query: 703 LTLNDTKTAVNKLHRMVNILVSVIILVIWLLILGIATSKFLLFVTSQLVLVAFVFGNTCK 762
LTLNDTKTAVN+LHR+V++LVS++IL+IWLLILGIAT+KFLL ++SQL+LV FVFGN+CK
Sbjct: 661 LTLNDTKTAVNRLHRIVDVLVSIVILIIWLLILGIATTKFLLVISSQLLLVVFVFGNSCK 720
Query: 763 TVFESIIFLFVMHPFDVGDRCEIDGVQMIVEEMNILTTIFLRYDNQKIIFPNSVLATKAI 822
T+FE++IF+FVMHPFDVGDRCEIDGVQMIVEEMNILTT+FLR+DNQKI++PNS+L TK I
Sbjct: 721 TIFEAVIFVFVMHPFDVGDRCEIDGVQMIVEEMNILTTVFLRFDNQKIVYPNSLLGTKPI 780
Query: 823 HNFYRSPDMGDGIEFCLHISTPPEKIAIMRQRIISYIEGKKEHWCPAPMIVLKDVEELNR 882
N+YRSPDM D IEF +HI+TPPEK +RQRI+SY++ KK+HW P+PMIV +D+ LN
Sbjct: 781 ANYYRSPDMQDAIEFFVHIATPPEKTTALRQRILSYVDNKKDHWHPSPMIVFRDMCGLNS 840
Query: 883 MRIAIWLTHRMNHQDMGERWTRRALLVEELVKIFQELDLQYRLLPLDINVRSLP---PVN 922
++IA+W TH+MNHQ+MGER+ RR L+EE+ ++ +ELD++YRL PL+INV+SLP P+
Sbjct: 841 VKIAMWPTHKMNHQNMGERYVRRGQLLEEIGRLCRELDIEYRLYPLNINVKSLPAATPIT 872
BLAST of Cucsa.119450 vs. Swiss-Prot
Match:
MSL6_ARATH (Mechanosensitive ion channel protein 6 OS=Arabidopsis thaliana GN=MSL6 PE=1 SV=1)
HSP 1 Score: 934.5 bits (2414), Expect = 9.0e-271
Identity = 477/747 (63.86%), Postives = 586/747 (78.45%), Query Frame = 1
Query: 193 SPLDEEHRGQQPRQ-CDRRAHGSNGEDDGAAEVLRCSSNSFFQRDVSFQRKSS-LLRAKT 252
+P+ E R R RR SN ++ EV++CS N+ QR SS LL+ +T
Sbjct: 119 TPVSESPRVSTKRDPVGRRDSRSNTNNNDDGEVVKCSGNN-----APIQRSSSTLLKMRT 178
Query: 253 KSRLLDPPEHQ------DRRSGRVPKSGQVRSGLISKAL----DEEDDDPFLEEDLPDEY 312
+SRL DPP Q D +SGR+PKSGQ++SG K+ +EE+DDPF EDLP+EY
Sbjct: 179 RSRLSDPPTPQLPPQTADMKSGRIPKSGQMKSGFFGKSPKTQGEEEEDDPFAAEDLPEEY 238
Query: 313 KKANLGVLTLLQWASLILIIAALVCTLTIRYWRRKKLWKLEVWKWEVMILVLICGRLVSG 372
+K L + +L+W SLILIIA VCTL I R+KKLW+L++WKWE M+LVLICGRLVS
Sbjct: 239 RKDKLSLWIVLEWLSLILIIAGFVCTLAIPSLRKKKLWELQLWKWESMVLVLICGRLVSS 298
Query: 373 WGIRVIVFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLFDDKVQREVKSNA 432
W ++++VFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGLVL+AW+FLFD+KV + + A
Sbjct: 299 WIVKIVVFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLLAWHFLFDEKVAKAANTKA 358
Query: 433 LEYVTKVLVCLLVSTLVWLVKTLMVKVLASSFHVSTYFDRIQDALFNQYVIETLSGPPLI 492
L VTK+ VCLLV L+WLVKTL+VKVLASSFH+STYFDRIQ++LF QYVIETLSGPPLI
Sbjct: 359 LRVVTKIFVCLLVGFLLWLVKTLLVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPLI 418
Query: 493 EIQKNEEEEERLAEEVIKLQNAGATIPPDLKATAFSTAQKGGRVIGSGGLQKSPR--GRS 552
EIQKNEEEEER++ EV K QN G V G QKSP G+S
Sbjct: 419 EIQKNEEEEERISVEVKKFQNPGG-------------------VEIQSGAQKSPMKTGKS 478
Query: 553 GKLSRTLSKKGG----DEGITIDHLHKLSPKNVSAWNMKRLMNIVRHGTLSTLDEQIKDT 612
LS LS GG ++GITID LHKL+PKNVSAW MKRLMNI+R+G+L+TLDEQ++D
Sbjct: 479 PFLSHVLSNGGGGGGENKGITIDSLHKLNPKNVSAWKMKRLMNIIRNGSLTTLDEQLQDP 538
Query: 613 AHEDESTTEIKSEYEAKVAAKKIFLNVARNGSKYIYLEDLMRFMEKDEASKTMGLFEGAC 672
+ +D+ +I+SE+EAK+AA+KIF NVA+ GSK+IY D+MRF+ DEA KT+ LFEGA
Sbjct: 539 SLDDDKGNQIRSEFEAKLAARKIFHNVAKPGSKFIYANDIMRFLPDDEALKTLSLFEGAS 598
Query: 673 ESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVIILVIWLLILGI 732
E+ +ISKSSLKNWVVNAFRERRALALTLNDTKTAVN+LH+MVNI+V +IILVIWL+ILGI
Sbjct: 599 ETNRISKSSLKNWVVNAFRERRALALTLNDTKTAVNRLHKMVNIVVGIIILVIWLIILGI 658
Query: 733 ATSKFLLFVTSQLVLVAFVFGNTCKTVFESIIFLFVMHPFDVGDRCEIDGVQMIVEEMNI 792
++KFL+ ++SQ+V+VAF+FGN CK VFESII+LFV+HPFDVGDRCEIDGVQM+VEEMNI
Sbjct: 659 TSTKFLVVMSSQVVVVAFIFGNMCKIVFESIIYLFVIHPFDVGDRCEIDGVQMVVEEMNI 718
Query: 793 LTTIFLRYDNQKIIFPNSVLATKAIHNFYRSPDMGDGIEFCLHISTPPEKIAIMRQRIIS 852
LTT+FLR+DNQK+++PNS+L TK+I N+YRSPDMGDGIEF +HI+TP EKI +++QRI S
Sbjct: 719 LTTVFLRFDNQKVVYPNSLLWTKSIGNYYRSPDMGDGIEFSIHITTPAEKIILIKQRITS 778
Query: 853 YIEGKKEHWCPAPMIVLKDVEELNRMRIAIWLTHRMNHQDMGERWTRRALLVEELVKIFQ 912
YIEGKK+HW PAPMIV KD+E LN +RIA+W THRMNHQDMGE+W RR+ LVEE+ KI +
Sbjct: 779 YIEGKKDHWYPAPMIVFKDMESLNSVRIAVWPTHRMNHQDMGEKWARRSQLVEEIAKICR 838
Query: 913 ELDLQYRLLPLDINVRSLPPVNSTNLP 922
ELD++YRL PLDINVR+LP ST LP
Sbjct: 839 ELDIEYRLYPLDINVRNLP--TSTALP 839
HSP 2 Score: 99.4 bits (246), Expect = 2.2e-19
Identity = 67/158 (42.41%), Postives = 88/158 (55.70%), Query Frame = 1
Query: 43 MAVSDSSDRKEVIVKVDEADASTLRATDLVNGS--GTIWRESSYDFWNDSDNRRNYGEGG 102
MAV D++DR+EVIVK+D + + + V+G G IWR+ SYDFW D + N G
Sbjct: 1 MAV-DAADRREVIVKIDGENGNN----NGVSGETVGKIWRDGSYDFWTDGEGNLNKGHNA 60
Query: 103 AKTTDD--------------FEFRQHRKDVEDPPSKLIGQFLHKQKASGEMSLDMDMEML 162
A D FEFR+ EDPP+KLIGQFLHKQ+ASGE+ LDMD+ M
Sbjct: 61 AAVDSDRSAATTGEQQKDEGFEFRRG----EDPPTKLIGQFLHKQQASGEICLDMDLGMD 120
Query: 163 ELPQDKTPLSTVAESPMRRSSRELKVSFESISEISEND 185
EL L+ V+ESP + R+ +S S + ND
Sbjct: 121 EL--QSRGLTPVSESPRVSTKRDPVGRRDSRSNTNNND 147
BLAST of Cucsa.119450 vs. Swiss-Prot
Match:
MSL7_ARATH (Mechanosensitive ion channel protein 7 OS=Arabidopsis thaliana GN=MSL7 PE=3 SV=1)
HSP 1 Score: 868.2 bits (2242), Expect = 7.9e-251
Identity = 471/920 (51.20%), Postives = 631/920 (68.59%), Query Frame = 1
Query: 6 KSFKSHPSYKYVRKLSGADATQDHEHLPILSDHQHSIMAVSDSSDRKEVIVKVDEADAST 65
K FKSH SYK + +S D ++ + +K+ + +D+ D +
Sbjct: 5 KPFKSHSSYKQI--ISTGDQ--------------------NEKTKKKKKLANLDDGDIAK 64
Query: 66 LRATDLVNGSGTIWRESSYDFWNDSDNRRNYGEGGAKTTDDFEFRQHRKDVEDPPSKLIG 125
++ SG+ + +SY FW D +Y + G+ F+F Q+R+
Sbjct: 65 TQS------SGSSFDGNSYKFWQDIATD-DYTKSGS-----FDFPQYRE----------- 124
Query: 126 QFLHKQKASGEMSLDMDMEMLELPQDKTPLSTVAESPMRRSSRELKVSFESISEISENDS 185
E++LD++ E E V+ + S+E +V F+ S + N S
Sbjct: 125 ----------EITLDVNEETEETED-------VSNNNNLSGSKETRVFFKINSSGTNNMS 184
Query: 186 MRRRHRDSPLDEEHRGQQPRQCDRRAHGSNGEDDGAAEVLRCSSNSFFQRDVSFQRKSSL 245
R S R + +D V+RCSS RK+ L
Sbjct: 185 GSVRSCTSSTSFS-------SATMRLNLEQQLEDEGEVVVRCSS----------VRKTEL 244
Query: 246 L-RAKTKSRLLDPPEHQDRR-SGRVPKSGQVRSGLISKALD--EEDDDPFLEEDLPDEYK 305
+ RAK +SRL+DPP+ ++++ S + S Q+RSGL+ + D EE+DD EED+P EY+
Sbjct: 245 VSRAKARSRLIDPPQEEEQQYSSWIGTSDQLRSGLLGRHSDDIEEEDDSSAEEDVPVEYR 304
Query: 306 KANLGVLTLLQWASLILIIAALVCTLTIRYWRRKKLWKLEVWKWEVMILVLICGRLVSGW 365
K + +TLLQW SLI ++ ALV +L + WR LW L +WKWEV++LVLICGRLVSG
Sbjct: 305 KLKMDAITLLQWMSLIALVVALVLSLGLHTWRNATLWSLHLWKWEVVLLVLICGRLVSGC 364
Query: 366 GIRVIVFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLFDDKVQREVKSNAL 425
GIR+IVFFIERNFLLRKRVLYFVYGV+ AVQNCLWLGLVL+AW+FLFD KV++E +S+ L
Sbjct: 365 GIRIIVFFIERNFLLRKRVLYFVYGVKTAVQNCLWLGLVLLAWHFLFDKKVEKETQSDVL 424
Query: 426 EYVTKVLVCLLVSTLVWLVKTLMVKVLASSFHVSTYFDRIQDALFNQYVIETLSGPPLIE 485
++K+LVC L+ST++WL+KTL+VKVLASSFHVSTYFDRIQ+ALF+ Y+IETLSGPP++E
Sbjct: 425 LLMSKILVCFLLSTVLWLIKTLVVKVLASSFHVSTYFDRIQEALFHHYLIETLSGPPMLE 484
Query: 486 IQKNEEEEERLAEEVIKLQNAGATIPPDLKATAFSTAQKGGRVIGSGGLQKSPRGRSGKL 545
+ + EEEE+R +E+ K+Q GA + P+L + AF + G + + K
Sbjct: 485 LSRIEEEEDRTQDEIYKMQKGGADLSPELCSAAFPQEKSGSTM-------------NMKF 544
Query: 546 SRTLSKKGGDEGITIDHLHKLSPKNVSAWNMKRLMNIVRHGTLSTLDEQIKDTAHEDEST 605
S + K G D GIT+D LHK++ KNVSAWNMKRLM IVR+ +LSTLDEQ EDEST
Sbjct: 545 SPIIPKTGSDNGITMDDLHKMNQKNVSAWNMKRLMKIVRNVSLSTLDEQALQNTCEDEST 604
Query: 606 TEIKSEYEAKVAAKKIFLNVARNGSKYIYLEDLMRFMEKDEASKTMGLFEGACESRKISK 665
+I+SE EAK AA+KIF NVA+ G+K+IYLEDLMRF+ DEA KTM LFEGA ++KI+K
Sbjct: 605 RQIRSEKEAKAAARKIFKNVAQPGTKHIYLEDLMRFLRVDEAMKTMCLFEGALVTKKITK 664
Query: 666 SSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVIILVIWLLILGIATSKFLL 725
S+LKNW+VNAFRERRALALTLNDTKTAVNKLH M++ L +++I+VIWL++L IATSK+LL
Sbjct: 665 SALKNWLVNAFRERRALALTLNDTKTAVNKLHHMISFLTAIVIIVIWLILLEIATSKYLL 724
Query: 726 FVTSQLVLVAFVFGNTCKTVFESIIFLFVMHPFDVGDRCEIDGVQMIVEEMNILTTIFLR 785
F+TSQ+VL+AF+FGN+ KTVFESIIFLF++HP+DVGDR ID V+M+VEEMNILTT+FLR
Sbjct: 725 FLTSQVVLLAFMFGNSLKTVFESIIFLFIIHPYDVGDRLLIDTVEMVVEEMNILTTVFLR 784
Query: 786 YDNQKIIFPNSVLATKAIHNFYRSPDMGDGIEFCLHISTPPEKIAIMRQRIISYIEGKKE 845
DN KI++PN +L KAIHN+ RSPDMGD + C+HI+TPPEKIA ++QRI SYI+ K E
Sbjct: 785 ADNLKIVYPNILLWQKAIHNYNRSPDMGDEVTCCVHITTPPEKIAAIKQRISSYIDSKPE 832
Query: 846 HWCPAPMIVLKDVEELNRMRIAIWLTHRMNHQDMGERWTRRALLVEELVKIFQELDLQYR 905
+W P +++KDVE+LN +RIAIWL H++NHQ+MGER+TRRALL+EE++KI ELD+QYR
Sbjct: 845 YWYPKADVIVKDVEDLNIVRIAIWLCHKINHQNMGERFTRRALLIEEVIKILLELDIQYR 832
Query: 906 LLPLDINVRSLPPVNSTNLP 922
PLDINV+++P V S+ +P
Sbjct: 905 FHPLDINVKTMPTVVSSRVP 832
BLAST of Cucsa.119450 vs. TrEMBL
Match:
A0A0A0KE62_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G124170 PE=4 SV=1)
HSP 1 Score: 1834.3 bits (4750), Expect = 0.0e+00
Identity = 923/923 (100.00%), Postives = 923/923 (100.00%), Query Frame = 1
Query: 1 MDFSLKSFKSHPSYKYVRKLSGADATQDHEHLPILSDHQHSIMAVSDSSDRKEVIVKVDE 60
MDFSLKSFKSHPSYKYVRKLSGADATQDHEHLPILSDHQHSIMAVSDSSDRKEVIVKVDE
Sbjct: 1 MDFSLKSFKSHPSYKYVRKLSGADATQDHEHLPILSDHQHSIMAVSDSSDRKEVIVKVDE 60
Query: 61 ADASTLRATDLVNGSGTIWRESSYDFWNDSDNRRNYGEGGAKTTDDFEFRQHRKDVEDPP 120
ADASTLRATDLVNGSGTIWRESSYDFWNDSDNRRNYGEGGAKTTDDFEFRQHRKDVEDPP
Sbjct: 61 ADASTLRATDLVNGSGTIWRESSYDFWNDSDNRRNYGEGGAKTTDDFEFRQHRKDVEDPP 120
Query: 121 SKLIGQFLHKQKASGEMSLDMDMEMLELPQDKTPLSTVAESPMRRSSRELKVSFESISEI 180
SKLIGQFLHKQKASGEMSLDMDMEMLELPQDKTPLSTVAESPMRRSSRELKVSFESISEI
Sbjct: 121 SKLIGQFLHKQKASGEMSLDMDMEMLELPQDKTPLSTVAESPMRRSSRELKVSFESISEI 180
Query: 181 SENDSMRRRHRDSPLDEEHRGQQPRQCDRRAHGSNGEDDGAAEVLRCSSNSFFQRDVSFQ 240
SENDSMRRRHRDSPLDEEHRGQQPRQCDRRAHGSNGEDDGAAEVLRCSSNSFFQRDVSFQ
Sbjct: 181 SENDSMRRRHRDSPLDEEHRGQQPRQCDRRAHGSNGEDDGAAEVLRCSSNSFFQRDVSFQ 240
Query: 241 RKSSLLRAKTKSRLLDPPEHQDRRSGRVPKSGQVRSGLISKALDEEDDDPFLEEDLPDEY 300
RKSSLLRAKTKSRLLDPPEHQDRRSGRVPKSGQVRSGLISKALDEEDDDPFLEEDLPDEY
Sbjct: 241 RKSSLLRAKTKSRLLDPPEHQDRRSGRVPKSGQVRSGLISKALDEEDDDPFLEEDLPDEY 300
Query: 301 KKANLGVLTLLQWASLILIIAALVCTLTIRYWRRKKLWKLEVWKWEVMILVLICGRLVSG 360
KKANLGVLTLLQWASLILIIAALVCTLTIRYWRRKKLWKLEVWKWEVMILVLICGRLVSG
Sbjct: 301 KKANLGVLTLLQWASLILIIAALVCTLTIRYWRRKKLWKLEVWKWEVMILVLICGRLVSG 360
Query: 361 WGIRVIVFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLFDDKVQREVKSNA 420
WGIRVIVFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLFDDKVQREVKSNA
Sbjct: 361 WGIRVIVFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLFDDKVQREVKSNA 420
Query: 421 LEYVTKVLVCLLVSTLVWLVKTLMVKVLASSFHVSTYFDRIQDALFNQYVIETLSGPPLI 480
LEYVTKVLVCLLVSTLVWLVKTLMVKVLASSFHVSTYFDRIQDALFNQYVIETLSGPPLI
Sbjct: 421 LEYVTKVLVCLLVSTLVWLVKTLMVKVLASSFHVSTYFDRIQDALFNQYVIETLSGPPLI 480
Query: 481 EIQKNEEEEERLAEEVIKLQNAGATIPPDLKATAFSTAQKGGRVIGSGGLQKSPRGRSGK 540
EIQKNEEEEERLAEEVIKLQNAGATIPPDLKATAFSTAQKGGRVIGSGGLQKSPRGRSGK
Sbjct: 481 EIQKNEEEEERLAEEVIKLQNAGATIPPDLKATAFSTAQKGGRVIGSGGLQKSPRGRSGK 540
Query: 541 LSRTLSKKGGDEGITIDHLHKLSPKNVSAWNMKRLMNIVRHGTLSTLDEQIKDTAHEDES 600
LSRTLSKKGGDEGITIDHLHKLSPKNVSAWNMKRLMNIVRHGTLSTLDEQIKDTAHEDES
Sbjct: 541 LSRTLSKKGGDEGITIDHLHKLSPKNVSAWNMKRLMNIVRHGTLSTLDEQIKDTAHEDES 600
Query: 601 TTEIKSEYEAKVAAKKIFLNVARNGSKYIYLEDLMRFMEKDEASKTMGLFEGACESRKIS 660
TTEIKSEYEAKVAAKKIFLNVARNGSKYIYLEDLMRFMEKDEASKTMGLFEGACESRKIS
Sbjct: 601 TTEIKSEYEAKVAAKKIFLNVARNGSKYIYLEDLMRFMEKDEASKTMGLFEGACESRKIS 660
Query: 661 KSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVIILVIWLLILGIATSKFL 720
KSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVIILVIWLLILGIATSKFL
Sbjct: 661 KSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVIILVIWLLILGIATSKFL 720
Query: 721 LFVTSQLVLVAFVFGNTCKTVFESIIFLFVMHPFDVGDRCEIDGVQMIVEEMNILTTIFL 780
LFVTSQLVLVAFVFGNTCKTVFESIIFLFVMHPFDVGDRCEIDGVQMIVEEMNILTTIFL
Sbjct: 721 LFVTSQLVLVAFVFGNTCKTVFESIIFLFVMHPFDVGDRCEIDGVQMIVEEMNILTTIFL 780
Query: 781 RYDNQKIIFPNSVLATKAIHNFYRSPDMGDGIEFCLHISTPPEKIAIMRQRIISYIEGKK 840
RYDNQKIIFPNSVLATKAIHNFYRSPDMGDGIEFCLHISTPPEKIAIMRQRIISYIEGKK
Sbjct: 781 RYDNQKIIFPNSVLATKAIHNFYRSPDMGDGIEFCLHISTPPEKIAIMRQRIISYIEGKK 840
Query: 841 EHWCPAPMIVLKDVEELNRMRIAIWLTHRMNHQDMGERWTRRALLVEELVKIFQELDLQY 900
EHWCPAPMIVLKDVEELNRMRIAIWLTHRMNHQDMGERWTRRALLVEELVKIFQELDLQY
Sbjct: 841 EHWCPAPMIVLKDVEELNRMRIAIWLTHRMNHQDMGERWTRRALLVEELVKIFQELDLQY 900
Query: 901 RLLPLDINVRSLPPVNSTNLPQR 924
RLLPLDINVRSLPPVNSTNLPQR
Sbjct: 901 RLLPLDINVRSLPPVNSTNLPQR 923
BLAST of Cucsa.119450 vs. TrEMBL
Match:
W9QBV6_9ROSA (Mechanosensitive ion channel protein 8 OS=Morus notabilis GN=L484_006078 PE=4 SV=1)
HSP 1 Score: 1224.9 bits (3168), Expect = 0.0e+00
Identity = 653/966 (67.60%), Postives = 765/966 (79.19%), Query Frame = 1
Query: 1 MDFSLK-SFKSHPSYKYVRKLS--GADATQDHEHLPILSDHQ----HSIM---AVSD--- 60
MDFSLK SFKSH SYK RK+S G + HE LPILSDH H + ++D
Sbjct: 1 MDFSLKKSFKSHGSYKNSRKISAGGGNPDNSHEELPILSDHDFDDNHHVQHYRRLNDNGK 60
Query: 61 ----SSDRKEVIVKVDEADASTLRATDLVNGSGTIWRESSYDFWNDSDNRRNYGEGGAKT 120
S+ R EVIVK+DE A+D + WRE+SY W ++ N R+ G G
Sbjct: 61 MGGASTGRNEVIVKIDEGGNPPAAASDQSRNAENFWRENSYGAWKENANVRDGGGSG--- 120
Query: 121 TDDFEFRQHRKDVEDPPSKLIGQFLHKQKASGEMSLDMDMEMLELPQD-----KTPLSTV 180
+ F F Q EDPPSKLI QFLH+QKASG++SLDMD+EM EL + + L+ V
Sbjct: 121 -ESFHFAQ---TTEDPPSKLIRQFLHRQKASGDISLDMDLEMEELRRSAGSGGELDLTPV 180
Query: 181 AESPMR-RSSRELKVSFESI-----SEISENDSMRRRHRDSPLDEEHRGQQPRQCDRRAH 240
+ESP RSSRELKVS SEIS+ND++RRR +DSP D + +RR
Sbjct: 181 SESPQNSRSSRELKVSLHPSPSDVGSEISQNDAVRRRFKDSPNDNDE------SLERR-- 240
Query: 241 GSNGEDDGAAEVLRCSSNSFFQRDVSFQRKSSLLRAKTKSRLLDPPEHQDRRSGRVPKSG 300
E DG EV+RC+SN+ FQR++SFQR+SS LR KT+SRL DPPE D RSGR+PKSG
Sbjct: 241 ----ERDGE-EVVRCTSNASFQREISFQRRSSFLRVKTRSRLQDPPEEPDMRSGRIPKSG 300
Query: 301 QV------------RSGLISKALDEEDDDPFLEEDLPDEYKKANLGVLTLLQWASLILII 360
Q+ +SGL+ K DEE+DDPF +EDLPDE+KKANL LTLLQWASLILI+
Sbjct: 301 QIPRSGPIPRSGPMKSGLLGK--DEEEDDPFSDEDLPDEFKKANLSALTLLQWASLILIV 360
Query: 361 AALVCTLTIRYWRRKKLWKLEVWKWEVMILVLICGRLVSGWGIRVIVFFIERNFLLRKRV 420
CTL+I Y R K L KL++WKWEV+ILVLICGRLVSGWGIR+ VFF ERNFLLRKRV
Sbjct: 361 GLFACTLSIHYLRHKNLLKLKLWKWEVLILVLICGRLVSGWGIRIAVFFFERNFLLRKRV 420
Query: 421 LYFVYGVRKAVQNCLWLGLVLIAWNFLFDDKVQREVKSNALEYVTKVLVCLLVSTLVWLV 480
LYFVYGVRKAVQNCLWLGLVLIAW+FLFD +V+RE +S +L+YVTKVL+C LV TL+WLV
Sbjct: 421 LYFVYGVRKAVQNCLWLGLVLIAWHFLFDKRVERETRSESLKYVTKVLICFLVGTLLWLV 480
Query: 481 KTLMVKVLASSFHVSTYFDRIQDALFNQYVIETLSGPPLIEIQKNEEEEERLAEEVIKLQ 540
KTL+VKVLASSFHVSTYFDRIQD+LFNQ+VIETLSGPPLIEI K EEEEERLA+EV KLQ
Sbjct: 481 KTLIVKVLASSFHVSTYFDRIQDSLFNQFVIETLSGPPLIEIYKTEEEEERLADEVEKLQ 540
Query: 541 NAGATIPPDLKATAFSTA-QKGGRVIGSGGLQKSPRGRSGKLSRTLSKKGGDEGITIDHL 600
NAGATIPPDL AFS+A + RVIGSG L ++ +S KLSR+LSK G DEGITIDHL
Sbjct: 541 NAGATIPPDL---AFSSALPRTSRVIGSGRLSRT--WKSSKLSRSLSKIG-DEGITIDHL 600
Query: 601 HKLSPKNVSAWNMKRLMNIVRHGTLSTLDEQIKDTAHEDESTTEIKSEYEAKVAAKKIFL 660
HKL+PKNVSAWNMKRLM +VRHGTL+TLDEQI D+ HEDES T+I+SE EAK AAKKIF
Sbjct: 601 HKLNPKNVSAWNMKRLMRMVRHGTLTTLDEQIVDSTHEDESATQIRSEVEAKAAAKKIFQ 660
Query: 661 NVARNGSKYIYLEDLMRFMEKDEASKTMGLFEGACESRKISKSSLKNWVVNAFRERRALA 720
NVAR GSK+I LEDLMRFM +DEA KTM LFEGA ES++ISKSSLKNWVVNAFRERRALA
Sbjct: 661 NVARRGSKFICLEDLMRFMREDEAVKTMSLFEGASESQRISKSSLKNWVVNAFRERRALA 720
Query: 721 LTLNDTKTAVNKLHRMVNILVSVIILVIWLLILGIATSKFLLFVTSQLVLVAFVFGNTCK 780
LTLNDTKTAVNKLHR+VN++V+++I VIWLLILGIATSKFLLF++SQLVLVAF+FGNTCK
Sbjct: 721 LTLNDTKTAVNKLHRIVNVIVAIVIGVIWLLILGIATSKFLLFISSQLVLVAFIFGNTCK 780
Query: 781 TVFESIIFLFVMHPFDVGDRCEIDGVQMIVEEMNILTTIFLRYDNQKIIFPNSVLATKAI 840
TVFE+I+FLFVMHPFDVGDRCEIDGVQM+VEEMNILTT+FLRYDN KIIFPNSVLATKAI
Sbjct: 781 TVFEAIVFLFVMHPFDVGDRCEIDGVQMVVEEMNILTTVFLRYDNAKIIFPNSVLATKAI 840
Query: 841 HNFYRSPDMGDGIEFCLHISTPPEKIAIMRQRIISYIEGKKEHWCPAPMIVLKDVEELNR 900
NFYRSPDMGDGIEF +H++TP +KIA MRQRI +Y+E KKEHW PAPM+++KD++ELNR
Sbjct: 841 DNFYRSPDMGDGIEFSIHVATPADKIAAMRQRITNYVENKKEHWYPAPMVIMKDLDELNR 900
Query: 901 MRIAIWLTHRMNHQDMGERWTRRALLVEELVKIFQELDLQYRLLPLDINVRSLPPV---- 922
+R+A+WL HR+NHQDMGER+ RR+LL+EE+VKIFQELD+QYRLLP+DINVR++P V
Sbjct: 901 VRMAVWLCHRINHQDMGERYARRSLLIEEMVKIFQELDIQYRLLPIDINVRAMPSVAPVP 938
BLAST of Cucsa.119450 vs. TrEMBL
Match:
M5VK71_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa001020mg PE=4 SV=1)
HSP 1 Score: 1197.6 bits (3097), Expect = 0.0e+00
Identity = 629/965 (65.18%), Postives = 743/965 (76.99%), Query Frame = 1
Query: 1 MDFSLK-SFKSHPSYKYVRKLSGADATQDHEHLPILSDHQ---HSIMAVSDSSDRKEVIV 60
MDFS+K SFKSH S K++RK+S E LPIL DH M+ DSSDR+EVIV
Sbjct: 1 MDFSIKKSFKSHGSAKHMRKISAGGDDPSLEQLPILLDHDSRHRQPMSPVDSSDRREVIV 60
Query: 61 KVDEADASTLRAT------DLVNGSGTIWRESSYDFWNDSDNRRNYGEGGAKTTDDFEFR 120
K+D+ ++S+ T D G IWRESS DFWN E G K F+F
Sbjct: 61 KIDDGESSSSATTRDAMAADPAKNGGKIWRESSVDFWN---------EDGVKNGQGFDFA 120
Query: 121 QHRKDVEDPPSKLIGQFLHKQKASGEMSLDMDMEMLELPQDKTPLSTVAESPMR-RSSRE 180
Q RK EDPPSKLIGQFLHKQ+ASG+MSLDMD+EM EL Q++ L VAESP R S+E
Sbjct: 121 QRRKTAEDPPSKLIGQFLHKQRASGDMSLDMDLEMEELRQNERDLPPVAESPRNSRVSKE 180
Query: 181 LKVSFESISEIS---ENDSMRRRHRDSPLDEEHRGQQPRQCDRRAHGSNGEDDGAAEVLR 240
LKVSF++ + S N+S+RRR+RDSP DE R + S+G+DD V+R
Sbjct: 181 LKVSFQAPAPDSVETPNESVRRRYRDSPDDERRRSGKL---------SDGQDD----VVR 240
Query: 241 CSSNSFFQRDVSFQRK--SSLLRAKTKSRLLDPPEHQDRRSGRVPKSGQV---------- 300
C+SN+ F+R+ SF K S LLR KT+SRL+DPPE D RSGR+P+SGQ+
Sbjct: 241 CTSNASFRREPSFANKNRSDLLRIKTRSRLMDPPEEPDFRSGRIPRSGQIPKSGQIPKSG 300
Query: 301 --------RSGLISKALDEEDDDPFLEEDLPDEYKKANLGVLTLLQWASLILIIAALVCT 360
+SG++ + D++DDDPFLEED+P EYK+A LTLLQW SL+LI+ AL+CT
Sbjct: 301 HIPKSGPMKSGMLGRGGDDDDDDPFLEEDVPYEYKRAKFNALTLLQWVSLVLIVGALICT 360
Query: 361 LTIRYWRRKKLWKLEVWKWEVMILVLICGRLVSGWGIRVIVFFIERNFLLRKRVLYFVYG 420
LTI R K LWKL++WKWEV+ILVLICGRLVSGWGIR+IV+F+ERNFLLRKRVLYFVYG
Sbjct: 361 LTIPVLRLKSLWKLKLWKWEVLILVLICGRLVSGWGIRIIVYFVERNFLLRKRVLYFVYG 420
Query: 421 VRKAVQNCLWLGLVLIAWNFLFDDKVQREVKSNALEYVTKVLVCLLVSTLVWLVKTLMVK 480
VR+AVQNCLWLGLVLIAW+F+FD KV+RE KS AL YVTKVL CLL+ L+WLVKTL+VK
Sbjct: 421 VRRAVQNCLWLGLVLIAWHFMFDKKVERETKSEALAYVTKVLFCLLIGVLLWLVKTLIVK 480
Query: 481 VLASSFHVSTYFDRIQDALFNQYVIETLSGPPLIEIQKNEEEEERLAEEVIKLQNAGATI 540
VLASSFHV +YFDRIQD+LFNQYVIETLSG PLIE+Q +EEEERLA+EV KLQNAGAT+
Sbjct: 481 VLASSFHVRSYFDRIQDSLFNQYVIETLSGRPLIEMQIEDEEEERLADEVRKLQNAGATM 540
Query: 541 PPDLKATAFSTAQKG----------GRVIGSGGLQKSPRGRSGKLSRTLSKKGGDEGITI 600
PPDLKA AF +A+ G GRVI SGGL G+S K SR LSKK + GITI
Sbjct: 541 PPDLKANAFPSARIGKVIGSGSLRSGRVIASGGLI----GKSTKFSRPLSKKSEETGITI 600
Query: 601 DHLHKLSPKNVSAWNMKRLMNIVRHGTLSTLDEQIKDTAHEDESTTEIKSEYEAKVAAKK 660
DHLHKL+PKNVSAWNMKRL+N+VR G L+TLDEQI SE EAK AAKK
Sbjct: 601 DHLHKLNPKNVSAWNMKRLINMVRKGHLTTLDEQI--------------SEVEAKAAAKK 660
Query: 661 IFLNVARNGSKYIYLEDLMRFMEKDEASKTMGLFEGACESRKISKSSLKNWVVNAFRERR 720
IF NVAR GSKYIYLEDLM FME+DEA KTM LFEGA E+R+ISKSSLKNWVV+AFRERR
Sbjct: 661 IFQNVARRGSKYIYLEDLMCFMEEDEAVKTMSLFEGAAENRRISKSSLKNWVVSAFRERR 720
Query: 721 ALALTLNDTKTAVNKLHRMVNILVSVIILVIWLLILGIATSKFLLFVTSQLVLVAFVFGN 780
ALALTLNDTKTAVN LHRMVNI+V++ I+VIWL+++GIAT+KFLLFV+SQLV+VAFVFGN
Sbjct: 721 ALALTLNDTKTAVNTLHRMVNIIVAIAIVVIWLIVMGIATTKFLLFVSSQLVVVAFVFGN 780
Query: 781 TCKTVFESIIFLFVMHPFDVGDRCEIDGVQMIVEEMNILTTIFLRYDNQKIIFPNSVLAT 840
TCKT+FE++IFLFVMHPFDVGDRCEI+GVQM+VEEMNILTT+FLRYDN KI +PNS+LAT
Sbjct: 781 TCKTIFEAMIFLFVMHPFDVGDRCEINGVQMVVEEMNILTTVFLRYDNTKITYPNSILAT 840
Query: 841 KAIHNFYRSPDMGDGIEFCLHISTPPEKIAIMRQRIISYIEGKKEHWCPAPMIVLKDVEE 900
I NFYRSPD GD IEF +HISTPP+KI +MRQRI+S+IE KKEHW P PMI++KDVEE
Sbjct: 841 LPIFNFYRSPDTGDAIEFSIHISTPPDKIVMMRQRIVSFIEDKKEHWYPGPMIIMKDVEE 900
Query: 901 LNRMRIAIWLTHRMNHQDMGERWTRRALLVEELVKIFQELDLQYRLLPLDINVRSLPPVN 922
LNR++ A+W THRMN QDMGERW RRA +VEE+V+IFQELD+QYRLLPLDINVR++PP+
Sbjct: 901 LNRIKFAVWPTHRMNFQDMGERWVRRAYVVEEMVRIFQELDIQYRLLPLDINVRAMPPMT 925
BLAST of Cucsa.119450 vs. TrEMBL
Match:
A0A0A0K619_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G017150 PE=4 SV=1)
HSP 1 Score: 1188.3 bits (3073), Expect = 0.0e+00
Identity = 608/942 (64.54%), Postives = 749/942 (79.51%), Query Frame = 1
Query: 1 MDFSLKSFKSHPSYKYVRKLS--GADATQDHEHLPILSDHQ---HSI-------MAVSDS 60
MD KSFK + S+K+ RK+S GA + +HE LPIL +HQ HS + SD+
Sbjct: 1 MDTLKKSFKGNVSFKHTRKISAGGAGSEINHEELPILLNHQTTDHSRCLRDRHPVNDSDT 60
Query: 61 SDRKEVIVKVDEADASTLRATDLV---NGSGTIWRESSYDFWNDSD-----NRRNYGEGG 120
SDR EVI+K+D +S+ + L N G +WRES YDFWN+ + + R G
Sbjct: 61 SDRTEVILKIDGGGSSSAVSRSLDSAGNNGGNVWRESRYDFWNNDEIGIGESARMGDNSG 120
Query: 121 AKTTDDFEFRQHRKDVEDPPSKLIGQFLHKQKASGEMSLDMDMEMLELPQDKTPLSTVAE 180
+ FEF Q +EDPP+KLIG FL KQK GE +LDMD+EM EL ++ + +AE
Sbjct: 121 VDRNEGFEFVQTGYGMEDPPTKLIGDFLCKQKIEGETTLDMDLEMEELKPNRI-VPPLAE 180
Query: 181 SPMRRSSRELKVSFESIS-EISEND-SMRRRHRDS-PLDEEHRG-QQPRQCDRRAHG--- 240
SP+ ++S++LKVSF+ S EIS ND S+RRR+RDS L EE +G Q PRQ
Sbjct: 181 SPLSQTSKDLKVSFQHDSTEISSNDQSIRRRNRDSNDLKEESKGGQSPRQQPHHERLGSP 240
Query: 241 --SNGEDDGAAEVLRCSSNSFFQRDVSFQRKSSLLRAKTKSRLLDPPEHQDRRSGRVPKS 300
S +++ AE +RC+SN F ++SFQRKS+LLRAKTKSRL+DPP DR SG +PKS
Sbjct: 241 TISGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLIDPPAEPDRLSGLIPKS 300
Query: 301 GQVRSGLISKALDEEDDDPFLEEDLPDEYKKANLGVLTLLQWASLILIIAALVCTLTIRY 360
GQ+RSG + K ++++DDDPFLE+DLPDE+K+ N LT+LQW SLI+I AAL+CTL++ Y
Sbjct: 301 GQLRSGFLGK-IEDDDDDPFLEDDLPDEFKRGNFSALTVLQWVSLIIITAALICTLSVPY 360
Query: 361 WRRKKLWKLEVWKWEVMILVLICGRLVSGWGIRVIVFFIERNFLLRKRVLYFVYGVRKAV 420
R K LW+L++WKWEVMI +LICGRLVSGWGIR+ VFFIERNFLLRKRVLYFVYGVRK V
Sbjct: 361 LREKSLWELDIWKWEVMIFILICGRLVSGWGIRIGVFFIERNFLLRKRVLYFVYGVRKPV 420
Query: 421 QNCLWLGLVLIAWNFLFDDKVQREVKSNALEYVTKVLVCLLVSTLVWLVKTLMVKVLASS 480
QNCLWLGLVLIAW+ LF+ +V+++ ++ L YV++VLVCLL+STL+WLVKTLMVKVLASS
Sbjct: 421 QNCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKVLASS 480
Query: 481 FHVSTYFDRIQDALFNQYVIETLSGPPLIEIQKNEEEEERLAEEVIKLQNAGATIPPDLK 540
FHVSTYFDRIQ++LFNQYVIETLSGPPL+EI+KNEEEEER+A+EV KLQNAG IPPDLK
Sbjct: 481 FHVSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGIIIPPDLK 540
Query: 541 ATAFSTAQKGGRVIGSGGLQKSPRGRSGKLSRTLSKKGGDEGITIDHLHKLSPKNVSAWN 600
A F++ K GR IGSG KS +S KLSR L+K D GITIDHLHKLS KNVSAWN
Sbjct: 541 AATFASI-KSGREIGSGRTHKSFCAKSCKLSRALTKNRND-GITIDHLHKLSTKNVSAWN 600
Query: 601 MKRLMNIVRHGTLSTLDEQIKDTAHEDESTTEIKSEYEAKVAAKKIFLNVARNGSKYIYL 660
MKRL+NIVR+G++STLDEQI+ +DESTTEIKSE EAK AAKKIF NVA G KYIYL
Sbjct: 601 MKRLLNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVAHRGYKYIYL 660
Query: 661 EDLMRFMEKDEASKTMGLFEGACESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNK 720
+DL+RFM +DE KTM LFEGA E+++ISKS+LKNWVVNAFRERRALALTLNDTKTAV+K
Sbjct: 661 DDLVRFMREDEVLKTMSLFEGAAENQRISKSALKNWVVNAFRERRALALTLNDTKTAVDK 720
Query: 721 LHRMVNILVSVIILVIWLLILGIATSKFLLFVTSQLVLVAFVFGNTCKTVFESIIFLFVM 780
LH MVN++ ++IL++WL++LGIA+SKF +F++SQ+V+VAF+FGNTCKT+FE+IIFLFVM
Sbjct: 721 LHHMVNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFVM 780
Query: 781 HPFDVGDRCEIDGVQMIVEEMNILTTIFLRYDNQKIIFPNSVLATKAIHNFYRSPDMGDG 840
HPFDVGDRCEIDG+QM+VEEMNILTT+FLRYDN K+I PNSVLATK IHNFYRSPDMG+
Sbjct: 781 HPFDVGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVIIPNSVLATKLIHNFYRSPDMGES 840
Query: 841 IEFCLHISTPPEKIAIMRQRIISYIEGKKEHWCPAPMIVLKDVEELNRMRIAIWLTHRMN 900
IEF +HI+TP EKI M+ RIISYIEG KEHW PAPMIV KD++ LN++++A+WL+HRMN
Sbjct: 841 IEFLVHIATPAEKITAMKHRIISYIEGNKEHWYPAPMIVFKDIDGLNKVKLAVWLSHRMN 900
Query: 901 HQDMGERWTRRALLVEELVKIFQELDLQYRLLPLDINVRSLP 914
HQD GERW RR++LVEE+VK+ QELD+QYRLLP+DIN+RSLP
Sbjct: 901 HQDSGERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLP 938
BLAST of Cucsa.119450 vs. TrEMBL
Match:
A0A061FGA7_THECC (Mechanosensitive channel of small conductance-like 6, putative OS=Theobroma cacao GN=TCM_032110 PE=4 SV=1)
HSP 1 Score: 1182.9 bits (3059), Expect = 0.0e+00
Identity = 622/917 (67.83%), Postives = 731/917 (79.72%), Query Frame = 1
Query: 43 MAVSDSSDRKEVIVKVD----EADASTLRATDLVNGSGTIWRESSYDFWNDSD------- 102
MAV DS+DRKEVI+K++ E++ A G G IWRESSYDFW DS+
Sbjct: 1 MAV-DSADRKEVILKINDRGNESNIPAAAAAATATGGGKIWRESSYDFWKDSEKISSNWK 60
Query: 103 --NRRNYGEGGA-----KTTDDFEF-RQHRKDVEDPPSKLIGQFLHKQKASGEMSLDMDM 162
N G G + + ++ F+F R + +EDPPSKLIGQFLHKQKASGE+SLDMD+
Sbjct: 61 KENANMNGTGSSANNSSRESEGFDFMRSKQAAIEDPPSKLIGQFLHKQKASGEISLDMDL 120
Query: 163 EMLELPQDK----TPLSTVAESPMRRSSRELKVSFESISEISENDSMRRRHRD---SPLD 222
EM EL Q+ T L TVAESP ++ +VSFE N+++RRR SP
Sbjct: 121 EMDELQQEPPHHGTLLPTVAESPSPSAAAVPRVSFE-------NNAVRRRQSKGSASPGK 180
Query: 223 EEHRGQQPRQCDRRAHGSNGEDDGAAEVLRCSSNSFFQRDV--SFQRKSSLLRAKTKSRL 282
EE R + E DG V++CSSNS F+R SFQRKSSLL KTKSRL
Sbjct: 181 EESRDSK-------------EGDG---VVKCSSNSSFKRSEGGSFQRKSSLLATKTKSRL 240
Query: 283 LDPP--EHQDRRSGR--VPKSGQV-RSGLISKALDEEDDDPFLEEDLPDEYKKANLGVLT 342
+DPP E + RS + V KSGQ+ RSG + K+++EE+DDP LEEDLPDEYKK L VL
Sbjct: 241 MDPPTPEKGEPRSAKATVGKSGQIMRSGFLGKSMEEEEDDPLLEEDLPDEYKKDKLSVLV 300
Query: 343 LLQWASLILIIAALVCTLTIRYWRRKKLWKLEVWKWEVMILVLICGRLVSGWGIRVIVFF 402
LL+W SLILI+AA VC+LTI Y R K+LW L +WKWEV++LVLICGRLVSGW IR+IVFF
Sbjct: 301 LLEWLSLILIVAAFVCSLTIPYLRAKRLWSLMLWKWEVLVLVLICGRLVSGWIIRIIVFF 360
Query: 403 IERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLFDDKVQREVKSNALEYVTKVLV 462
IERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAW++LFD KVQRE KS L YVTKVLV
Sbjct: 361 IERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWHYLFDKKVQRETKSEFLRYVTKVLV 420
Query: 463 CLLVSTLVWLVKTLMVKVLASSFHVSTYFDRIQDALFNQYVIETLSGPPLIEIQKNEEEE 522
CL+V ++WLVKTL+VKVLASSFHVSTYFDRIQD+LFNQYV+ETLSGPPLIEIQ+ EEEE
Sbjct: 421 CLVVGVMLWLVKTLLVKVLASSFHVSTYFDRIQDSLFNQYVVETLSGPPLIEIQRAEEEE 480
Query: 523 ERLAEEVIKLQNAGATIPPDLKATAFSTAQKGGRVIGSGGLQKSPRGRSGKLSRTLSKKG 582
ER+A EV LQ AGATIPP LK + S+ G +VIGSG +QKSPRG+S LSR LS +
Sbjct: 481 ERIANEVKNLQKAGATIPPGLKTSTLSSPHSG-KVIGSGRIQKSPRGKSPMLSRMLSSEK 540
Query: 583 GDE---GITIDHLHKLSPKNVSAWNMKRLMNIVRHGTLSTLDEQIKDTAHEDESTTEIKS 642
G++ GITIDHLHKL+ KNVSAWNMKRLMNI+RHG LSTLDEQI+D+ HEDES T+I+S
Sbjct: 541 GEKDKKGITIDHLHKLNHKNVSAWNMKRLMNIIRHGALSTLDEQIQDSTHEDESATQIRS 600
Query: 643 EYEAKVAAKKIFLNVARNGSKYIYLEDLMRFMEKDEASKTMGLFEGACESRKISKSSLKN 702
EYEAKVAA+KIF NVA+ GSKYIYLED+ RF+++DEA KTM LFEGA ESR+ISK +LKN
Sbjct: 601 EYEAKVAARKIFQNVAKPGSKYIYLEDIERFLQEDEAFKTMSLFEGASESRRISKKALKN 660
Query: 703 WVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVIILVIWLLILGIATSKFLLFVTSQ 762
WVVNAFRERRALALTLNDTKTAVN+LHRMVN+LV +II+VIWLLILGIA+SK L+F++SQ
Sbjct: 661 WVVNAFRERRALALTLNDTKTAVNRLHRMVNVLVGIIIVVIWLLILGIASSKVLVFISSQ 720
Query: 763 LVLVAFVFGNTCKTVFESIIFLFVMHPFDVGDRCEIDGVQMIVEEMNILTTIFLRYDNQK 822
L+LVAF+FGNTCKTVFE+IIFLFVMHPFDVGDRCEIDGVQM+VEEMNILTT+FLRYDNQK
Sbjct: 721 LLLVAFIFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDGVQMVVEEMNILTTVFLRYDNQK 780
Query: 823 IIFPNSVLATKAIHNFYRSPDMGDGIEFCLHISTPPEKIAIMRQRIISYIEGKKEHWCPA 882
II PNSVLATKAI+N+YRSPDMGD +EFC+H+ TP EKI +M+QRI+SYIE K +HW PA
Sbjct: 781 IIIPNSVLATKAINNYYRSPDMGDAVEFCIHVKTPAEKIGLMKQRILSYIEHKSDHWYPA 840
Query: 883 PMIVLKDVEELNRMRIAIWLTHRMNHQDMGERWTRRALLVEELVKIFQELDLQYRLLPLD 924
PMI+ K++EELNR+RIAIWLTHRMNHQDMGERW RRALLVEE+VKIF +LD++YRL P+D
Sbjct: 841 PMIIFKELEELNRVRIAIWLTHRMNHQDMGERWARRALLVEEMVKIFNDLDIKYRLYPID 892
BLAST of Cucsa.119450 vs. TAIR10
Match:
AT3G14810.1 (AT3G14810.1 mechanosensitive channel of small conductance-like 5)
HSP 1 Score: 979.5 bits (2531), Expect = 1.4e-285
Identity = 513/907 (56.56%), Postives = 661/907 (72.88%), Query Frame = 1
Query: 43 MAVSDSSDRKEVIVKVDEADASTLRATDLVNGS--GTIWRESSYDFWNDSDNRRNYGEGG 102
MA DS+DR++ IV ++ ++ + AT + + G IW+ESSYDFW D + +N +G
Sbjct: 1 MAAVDSTDRRDFIVNINGQESGAVGATGSSSNAEGGNIWKESSYDFW-DGEKGKNDKKGD 60
Query: 103 AKTTD--DFEFRQHRK------DVEDPPSKLIGQFLHKQKASG-EMSLDMDMEMLELPQD 162
+ D F FRQ + ++ DPPSKLIGQFLHKQ+ASG E+SLD+++ M EL +
Sbjct: 61 DEDEDGGSFHFRQRGERRHSSAELSDPPSKLIGQFLHKQRASGDEISLDVELNMAELQSN 120
Query: 163 KTPLSTVAESPMRRSSRELKVSFESISEISENDSMRRRHRDSPL----DEEHRGQQPRQC 222
P A + RR + S + + D++RRR + L DEE R +
Sbjct: 121 TPPRPATASNTPRRGLTTISESSSPVKTKVKADAVRRRQNRTSLGGSSDEEGRNRDE--- 180
Query: 223 DRRAHGSNGEDDGAAEVLRCSSNSFFQRDVSFQRKSSLLRAKTKSRLLDPP-------EH 282
AEVL+C S +K L R KTKSRL DPP +
Sbjct: 181 --------------AEVLKCGS-----------KKPMLSRNKTKSRLQDPPTPTHPAIDK 240
Query: 283 QDRRSGRVPKSGQVRSGLISKAL-----------DEEDDDPFLEEDLPDEYKKANLGVLT 342
+ +SGR +SG +SG + K+ +EE++DPFL+EDLP+E+K+ L
Sbjct: 241 TEMKSGR--RSGIFKSGFLGKSPKAGTPGRNGFEEEEEEDPFLDEDLPEEFKRDKLSFWV 300
Query: 343 LLQWASLILIIAALVCTLTIRYWRRKKLWKLEVWKWEVMILVLICGRLVSGWGIRVIVFF 402
L+W SL+LI+ +LVC+LTI +RK WKL++WKWEV +LVLICGRLVS W +R+IVF
Sbjct: 301 FLEWISLVLIVTSLVCSLTIHNLQRKTWWKLDLWKWEVTVLVLICGRLVSSWIVRIIVFL 360
Query: 403 IERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLFDDKVQREVKSNALEYVTKVLV 462
+E+NF RKRVLYFVYGVRK+VQNCLWLGLVL+AW+FLFD KV+RE +S AL YVT+VLV
Sbjct: 361 VEKNFTWRKRVLYFVYGVRKSVQNCLWLGLVLLAWHFLFDKKVERETRSTALRYVTRVLV 420
Query: 463 CLLVSTLVWLVKTLMVKVLASSFHVSTYFDRIQDALFNQYVIETLSGPPLIEIQKNEEEE 522
CLLV+ ++WLVKT++VKVLASSFH+STYFDRIQ++LF QYVIETLSGPPL+EIQ+ EEEE
Sbjct: 421 CLLVALIIWLVKTILVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPLMEIQRMEEEE 480
Query: 523 ERLAEEVIKLQN-AGATIPPDLKATAFSTAQKGGRVIGSGGLQKSPRGRSGKLSRTLSKK 582
+++AE+V L+ AGA +PP LKAT S + G KSP L+R SK+
Sbjct: 481 QQVAEDVKSLEKLAGAKLPPALKATVKSFMKVG----------KSPG-----LNRIGSKR 540
Query: 583 GGD-EGITIDHLHKLSPKNVSAWNMKRLMNIVRHGTLSTLDEQIKDTAHEDESTTEIKSE 642
G D EGI ID L +++ KNVSAWNMKRLMNI+ G +STLD+ ++DT EDE T I+SE
Sbjct: 541 GEDGEGIRIDQLKRMNTKNVSAWNMKRLMNIILKGAISTLDQNMQDTTQEDEDATHIRSE 600
Query: 643 YEAKVAAKKIFLNVARNGSKYIYLEDLMRFMEKDEASKTMGLFEGACESRKISKSSLKNW 702
YEAK AA+KIF NV GS+YIYLED +RF+ ++EA + M LFEGA ES KISKS LKNW
Sbjct: 601 YEAKCAARKIFHNVTEPGSRYIYLEDFLRFLCEEEAERAMALFEGASESDKISKSCLKNW 660
Query: 703 VVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVIILVIWLLILGIATSKFLLFVTSQL 762
VV AFRERRALALTLNDTKTAV++LHR++N+++ +II++IWLLILGIAT++FLL ++SQL
Sbjct: 661 VVKAFRERRALALTLNDTKTAVDRLHRIINVVIGIIIIIIWLLILGIATTRFLLVLSSQL 720
Query: 763 VLVAFVFGNTCKTVFESIIFLFVMHPFDVGDRCEIDGVQMIVEEMNILTTIFLRYDNQKI 822
+LVAFVFGN+CKT+FE+IIFLFVMHPFDVGDRCEIDGVQ++VEEMNILTT+FLRYDNQKI
Sbjct: 721 LLVAFVFGNSCKTIFEAIIFLFVMHPFDVGDRCEIDGVQLVVEEMNILTTVFLRYDNQKI 780
Query: 823 IFPNSVLATKAIHNFYRSPDMGDGIEFCLHISTPPEKIAIMRQRIISYIEGKKEHWCPAP 882
I+PNSVL TK I N+YRSPDMGD +EFC+HI+TPPEKI ++QRI+SY++ KK++W PAP
Sbjct: 781 IYPNSVLGTKPIANYYRSPDMGDAVEFCVHIATPPEKITAIKQRILSYVDNKKDYWYPAP 840
Query: 883 MIVLKDVEELNRMRIAIWLTHRMNHQDMGERWTRRALLVEELVKIFQELDLQYRLLPLDI 915
MIV +++LN ++IA+WLTHRMNHQDMGER+ RR LL+EE+ K +ELD++YRL PL+I
Sbjct: 841 MIVFLSMDDLNSVKIAVWLTHRMNHQDMGERYIRRGLLLEEVGKTCRELDIEYRLYPLNI 861
BLAST of Cucsa.119450 vs. TAIR10
Match:
AT1G53470.1 (AT1G53470.1 mechanosensitive channel of small conductance-like 4)
HSP 1 Score: 943.0 bits (2436), Expect = 1.4e-274
Identity = 500/905 (55.25%), Postives = 649/905 (71.71%), Query Frame = 1
Query: 43 MAVSDSSDRKEVIVKVDEADASTLRATDLVNG-SGTIWRESSYDFW-NDSDNRRNYGEGG 102
MAV + R++ +V++D D NG S WRESS +FW ND ++ GE
Sbjct: 1 MAVDSTDQRRDFVVRIDGED----------NGDSEKFWRESSINFWHNDKSSKPPGGEED 60
Query: 103 AKTTDDFEFRQHRKDVEDPPSKLIGQFLHKQKASG-EMSLDMDMEMLELPQDKTP-LSTV 162
+ D + + DPPSKLI QFL+KQKASG E+SLDM+ M EL ++ P LS+
Sbjct: 61 DGSFDFMRRSSEKSEEPDPPSKLINQFLNKQKASGDEISLDMEANMPELQKNTVPPLSST 120
Query: 163 AESPMRRSSRELKVSFESISEISENDSMRRRHRDSPLDEEHR-GQQPRQCDRRAHGSNGE 222
A S S+ + + D++RRR L + G + R GS
Sbjct: 121 AVSG---SASPVTAPVTASYRNGTGDAIRRRQNRVTLSPSVKDGDSSEDEENRVDGS--- 180
Query: 223 DDGAAEVLRCSSNSFFQRDVSFQRKSSLLRAKTKSRLLDPPE--HQDRRSGRVPKSGQVR 282
EV++C+SN S R +L++ KT+SRL+DPP + D SGR P+SG +
Sbjct: 181 -----EVVKCTSNR------STMRTKTLMKMKTRSRLMDPPTPTYPDMVSGRTPRSGNLN 240
Query: 283 SGLISKALD------------EEDDDPFLEEDLPDEYKKANLGVLTLLQWASLILIIAAL 342
G + EE++DPF EEDLP+ +K + V +++W LILIIA+L
Sbjct: 241 PGFSGRNTKPGTPNQGGSKDLEEEEDPFSEEDLPEGLRKEKICVWVIIEWIFLILIIASL 300
Query: 343 VCTLTIRYWRRKKLWKLEVWKWEVMILVLICGRLVSGWGIRVIVFFIERNFLLRKRVLYF 402
+C+L I Y R K LW L +WKWEVM+LVLICGRLVS W +++ V+F+E NFL RK+VLYF
Sbjct: 301 ICSLVIPYLRGKTLWDLALWKWEVMVLVLICGRLVSSWIVKLFVYFVESNFLWRKKVLYF 360
Query: 403 VYGVRKAVQNCLWLGLVLIAWNFLFDDKVQREVKSNALEYVTKVLVCLLVSTLVWLVKTL 462
VYG+RK VQNCLWLGLVLIAW+FLFD KV+RE++S L+YVTKVL+CLLV+ ++WL+KTL
Sbjct: 361 VYGIRKPVQNCLWLGLVLIAWHFLFDKKVEREMRSTVLKYVTKVLICLLVAVIIWLIKTL 420
Query: 463 MVKVLASSFHVSTYFDRIQDALFNQYVIETLSGPPLIEIQKNEEEEERLAEEVIKLQNAG 522
+VKVLASSFH+STYFDRIQ++LF QYVIETLSGPP IEI EEE++A +V + G
Sbjct: 421 LVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPRIEIHI---EEEKVANDVKTFEIVG 480
Query: 523 ATIPPDLKATAFSTAQKGGRVIGSGGLQKSPR--GRSGKLSRTLSKK-GGDEGITIDHLH 582
+ P S Q +GSG LQKSP G+S LSR+ SKK GG+EGI IDHL
Sbjct: 481 RKLSPLGPKAVSSPPQV---TVGSGRLQKSPSRVGKSPVLSRSGSKKEGGEEGIRIDHLQ 540
Query: 583 KLSPKNVSAWNMKRLMNIVRHGTLSTLDEQIKDTA-HEDESTTEIKSEYEAKVAAKKIFL 642
+++ KNVSAW MK+LMN+++ GTLSTLDEQI+DT ED+ T+I+SE+EAK+AA+KIF
Sbjct: 541 RMNTKNVSAWKMKKLMNVIKKGTLSTLDEQIQDTTTQEDDKATQIRSEFEAKLAARKIFQ 600
Query: 643 NVARNGSKYIYLEDLMRFMEKDEASKTMGLFEGACESRKISKSSLKNWVVNAFRERRALA 702
NVA GS+YIY+ED MRF+ +DE+ + M LFEGA E KISKS LKNWVVNAFRERRALA
Sbjct: 601 NVAEPGSRYIYMEDFMRFLSEDESERAMDLFEGASECHKISKSCLKNWVVNAFRERRALA 660
Query: 703 LTLNDTKTAVNKLHRMVNILVSVIILVIWLLILGIATSKFLLFVTSQLVLVAFVFGNTCK 762
LTLNDTKTAVN+LHR+V++LVS++IL+IWLLILGIAT+KFLL ++SQL+LV FVFGN+CK
Sbjct: 661 LTLNDTKTAVNRLHRIVDVLVSIVILIIWLLILGIATTKFLLVISSQLLLVVFVFGNSCK 720
Query: 763 TVFESIIFLFVMHPFDVGDRCEIDGVQMIVEEMNILTTIFLRYDNQKIIFPNSVLATKAI 822
T+FE++IF+FVMHPFDVGDRCEIDGVQMIVEEMNILTT+FLR+DNQKI++PNS+L TK I
Sbjct: 721 TIFEAVIFVFVMHPFDVGDRCEIDGVQMIVEEMNILTTVFLRFDNQKIVYPNSLLGTKPI 780
Query: 823 HNFYRSPDMGDGIEFCLHISTPPEKIAIMRQRIISYIEGKKEHWCPAPMIVLKDVEELNR 882
N+YRSPDM D IEF +HI+TPPEK +RQRI+SY++ KK+HW P+PMIV +D+ LN
Sbjct: 781 ANYYRSPDMQDAIEFFVHIATPPEKTTALRQRILSYVDNKKDHWHPSPMIVFRDMCGLNS 840
Query: 883 MRIAIWLTHRMNHQDMGERWTRRALLVEELVKIFQELDLQYRLLPLDINVRSLP---PVN 922
++IA+W TH+MNHQ+MGER+ RR L+EE+ ++ +ELD++YRL PL+INV+SLP P+
Sbjct: 841 VKIAMWPTHKMNHQNMGERYVRRGQLLEEIGRLCRELDIEYRLYPLNINVKSLPAATPIT 872
BLAST of Cucsa.119450 vs. TAIR10
Match:
AT1G78610.1 (AT1G78610.1 mechanosensitive channel of small conductance-like 6)
HSP 1 Score: 934.5 bits (2414), Expect = 5.1e-272
Identity = 477/747 (63.86%), Postives = 586/747 (78.45%), Query Frame = 1
Query: 193 SPLDEEHRGQQPRQ-CDRRAHGSNGEDDGAAEVLRCSSNSFFQRDVSFQRKSS-LLRAKT 252
+P+ E R R RR SN ++ EV++CS N+ QR SS LL+ +T
Sbjct: 119 TPVSESPRVSTKRDPVGRRDSRSNTNNNDDGEVVKCSGNN-----APIQRSSSTLLKMRT 178
Query: 253 KSRLLDPPEHQ------DRRSGRVPKSGQVRSGLISKAL----DEEDDDPFLEEDLPDEY 312
+SRL DPP Q D +SGR+PKSGQ++SG K+ +EE+DDPF EDLP+EY
Sbjct: 179 RSRLSDPPTPQLPPQTADMKSGRIPKSGQMKSGFFGKSPKTQGEEEEDDPFAAEDLPEEY 238
Query: 313 KKANLGVLTLLQWASLILIIAALVCTLTIRYWRRKKLWKLEVWKWEVMILVLICGRLVSG 372
+K L + +L+W SLILIIA VCTL I R+KKLW+L++WKWE M+LVLICGRLVS
Sbjct: 239 RKDKLSLWIVLEWLSLILIIAGFVCTLAIPSLRKKKLWELQLWKWESMVLVLICGRLVSS 298
Query: 373 WGIRVIVFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLFDDKVQREVKSNA 432
W ++++VFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGLVL+AW+FLFD+KV + + A
Sbjct: 299 WIVKIVVFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLLAWHFLFDEKVAKAANTKA 358
Query: 433 LEYVTKVLVCLLVSTLVWLVKTLMVKVLASSFHVSTYFDRIQDALFNQYVIETLSGPPLI 492
L VTK+ VCLLV L+WLVKTL+VKVLASSFH+STYFDRIQ++LF QYVIETLSGPPLI
Sbjct: 359 LRVVTKIFVCLLVGFLLWLVKTLLVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPLI 418
Query: 493 EIQKNEEEEERLAEEVIKLQNAGATIPPDLKATAFSTAQKGGRVIGSGGLQKSPR--GRS 552
EIQKNEEEEER++ EV K QN G V G QKSP G+S
Sbjct: 419 EIQKNEEEEERISVEVKKFQNPGG-------------------VEIQSGAQKSPMKTGKS 478
Query: 553 GKLSRTLSKKGG----DEGITIDHLHKLSPKNVSAWNMKRLMNIVRHGTLSTLDEQIKDT 612
LS LS GG ++GITID LHKL+PKNVSAW MKRLMNI+R+G+L+TLDEQ++D
Sbjct: 479 PFLSHVLSNGGGGGGENKGITIDSLHKLNPKNVSAWKMKRLMNIIRNGSLTTLDEQLQDP 538
Query: 613 AHEDESTTEIKSEYEAKVAAKKIFLNVARNGSKYIYLEDLMRFMEKDEASKTMGLFEGAC 672
+ +D+ +I+SE+EAK+AA+KIF NVA+ GSK+IY D+MRF+ DEA KT+ LFEGA
Sbjct: 539 SLDDDKGNQIRSEFEAKLAARKIFHNVAKPGSKFIYANDIMRFLPDDEALKTLSLFEGAS 598
Query: 673 ESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVIILVIWLLILGI 732
E+ +ISKSSLKNWVVNAFRERRALALTLNDTKTAVN+LH+MVNI+V +IILVIWL+ILGI
Sbjct: 599 ETNRISKSSLKNWVVNAFRERRALALTLNDTKTAVNRLHKMVNIVVGIIILVIWLIILGI 658
Query: 733 ATSKFLLFVTSQLVLVAFVFGNTCKTVFESIIFLFVMHPFDVGDRCEIDGVQMIVEEMNI 792
++KFL+ ++SQ+V+VAF+FGN CK VFESII+LFV+HPFDVGDRCEIDGVQM+VEEMNI
Sbjct: 659 TSTKFLVVMSSQVVVVAFIFGNMCKIVFESIIYLFVIHPFDVGDRCEIDGVQMVVEEMNI 718
Query: 793 LTTIFLRYDNQKIIFPNSVLATKAIHNFYRSPDMGDGIEFCLHISTPPEKIAIMRQRIIS 852
LTT+FLR+DNQK+++PNS+L TK+I N+YRSPDMGDGIEF +HI+TP EKI +++QRI S
Sbjct: 719 LTTVFLRFDNQKVVYPNSLLWTKSIGNYYRSPDMGDGIEFSIHITTPAEKIILIKQRITS 778
Query: 853 YIEGKKEHWCPAPMIVLKDVEELNRMRIAIWLTHRMNHQDMGERWTRRALLVEELVKIFQ 912
YIEGKK+HW PAPMIV KD+E LN +RIA+W THRMNHQDMGE+W RR+ LVEE+ KI +
Sbjct: 779 YIEGKKDHWYPAPMIVFKDMESLNSVRIAVWPTHRMNHQDMGEKWARRSQLVEEIAKICR 838
Query: 913 ELDLQYRLLPLDINVRSLPPVNSTNLP 922
ELD++YRL PLDINVR+LP ST LP
Sbjct: 839 ELDIEYRLYPLDINVRNLP--TSTALP 839
HSP 2 Score: 99.4 bits (246), Expect = 1.3e-20
Identity = 67/158 (42.41%), Postives = 88/158 (55.70%), Query Frame = 1
Query: 43 MAVSDSSDRKEVIVKVDEADASTLRATDLVNGS--GTIWRESSYDFWNDSDNRRNYGEGG 102
MAV D++DR+EVIVK+D + + + V+G G IWR+ SYDFW D + N G
Sbjct: 1 MAV-DAADRREVIVKIDGENGNN----NGVSGETVGKIWRDGSYDFWTDGEGNLNKGHNA 60
Query: 103 AKTTDD--------------FEFRQHRKDVEDPPSKLIGQFLHKQKASGEMSLDMDMEML 162
A D FEFR+ EDPP+KLIGQFLHKQ+ASGE+ LDMD+ M
Sbjct: 61 AAVDSDRSAATTGEQQKDEGFEFRRG----EDPPTKLIGQFLHKQQASGEICLDMDLGMD 120
Query: 163 ELPQDKTPLSTVAESPMRRSSRELKVSFESISEISEND 185
EL L+ V+ESP + R+ +S S + ND
Sbjct: 121 EL--QSRGLTPVSESPRVSTKRDPVGRRDSRSNTNNND 147
BLAST of Cucsa.119450 vs. TAIR10
Match:
AT2G17000.1 (AT2G17000.1 Mechanosensitive ion channel family protein)
HSP 1 Score: 868.2 bits (2242), Expect = 4.5e-252
Identity = 471/920 (51.20%), Postives = 631/920 (68.59%), Query Frame = 1
Query: 6 KSFKSHPSYKYVRKLSGADATQDHEHLPILSDHQHSIMAVSDSSDRKEVIVKVDEADAST 65
K FKSH SYK + +S D ++ + +K+ + +D+ D +
Sbjct: 5 KPFKSHSSYKQI--ISTGDQ--------------------NEKTKKKKKLANLDDGDIAK 64
Query: 66 LRATDLVNGSGTIWRESSYDFWNDSDNRRNYGEGGAKTTDDFEFRQHRKDVEDPPSKLIG 125
++ SG+ + +SY FW D +Y + G+ F+F Q+R+
Sbjct: 65 TQS------SGSSFDGNSYKFWQDIATD-DYTKSGS-----FDFPQYRE----------- 124
Query: 126 QFLHKQKASGEMSLDMDMEMLELPQDKTPLSTVAESPMRRSSRELKVSFESISEISENDS 185
E++LD++ E E V+ + S+E +V F+ S + N S
Sbjct: 125 ----------EITLDVNEETEETED-------VSNNNNLSGSKETRVFFKINSSGTNNMS 184
Query: 186 MRRRHRDSPLDEEHRGQQPRQCDRRAHGSNGEDDGAAEVLRCSSNSFFQRDVSFQRKSSL 245
R S R + +D V+RCSS RK+ L
Sbjct: 185 GSVRSCTSSTSFS-------SATMRLNLEQQLEDEGEVVVRCSS----------VRKTEL 244
Query: 246 L-RAKTKSRLLDPPEHQDRR-SGRVPKSGQVRSGLISKALD--EEDDDPFLEEDLPDEYK 305
+ RAK +SRL+DPP+ ++++ S + S Q+RSGL+ + D EE+DD EED+P EY+
Sbjct: 245 VSRAKARSRLIDPPQEEEQQYSSWIGTSDQLRSGLLGRHSDDIEEEDDSSAEEDVPVEYR 304
Query: 306 KANLGVLTLLQWASLILIIAALVCTLTIRYWRRKKLWKLEVWKWEVMILVLICGRLVSGW 365
K + +TLLQW SLI ++ ALV +L + WR LW L +WKWEV++LVLICGRLVSG
Sbjct: 305 KLKMDAITLLQWMSLIALVVALVLSLGLHTWRNATLWSLHLWKWEVVLLVLICGRLVSGC 364
Query: 366 GIRVIVFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLFDDKVQREVKSNAL 425
GIR+IVFFIERNFLLRKRVLYFVYGV+ AVQNCLWLGLVL+AW+FLFD KV++E +S+ L
Sbjct: 365 GIRIIVFFIERNFLLRKRVLYFVYGVKTAVQNCLWLGLVLLAWHFLFDKKVEKETQSDVL 424
Query: 426 EYVTKVLVCLLVSTLVWLVKTLMVKVLASSFHVSTYFDRIQDALFNQYVIETLSGPPLIE 485
++K+LVC L+ST++WL+KTL+VKVLASSFHVSTYFDRIQ+ALF+ Y+IETLSGPP++E
Sbjct: 425 LLMSKILVCFLLSTVLWLIKTLVVKVLASSFHVSTYFDRIQEALFHHYLIETLSGPPMLE 484
Query: 486 IQKNEEEEERLAEEVIKLQNAGATIPPDLKATAFSTAQKGGRVIGSGGLQKSPRGRSGKL 545
+ + EEEE+R +E+ K+Q GA + P+L + AF + G + + K
Sbjct: 485 LSRIEEEEDRTQDEIYKMQKGGADLSPELCSAAFPQEKSGSTM-------------NMKF 544
Query: 546 SRTLSKKGGDEGITIDHLHKLSPKNVSAWNMKRLMNIVRHGTLSTLDEQIKDTAHEDEST 605
S + K G D GIT+D LHK++ KNVSAWNMKRLM IVR+ +LSTLDEQ EDEST
Sbjct: 545 SPIIPKTGSDNGITMDDLHKMNQKNVSAWNMKRLMKIVRNVSLSTLDEQALQNTCEDEST 604
Query: 606 TEIKSEYEAKVAAKKIFLNVARNGSKYIYLEDLMRFMEKDEASKTMGLFEGACESRKISK 665
+I+SE EAK AA+KIF NVA+ G+K+IYLEDLMRF+ DEA KTM LFEGA ++KI+K
Sbjct: 605 RQIRSEKEAKAAARKIFKNVAQPGTKHIYLEDLMRFLRVDEAMKTMCLFEGALVTKKITK 664
Query: 666 SSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVIILVIWLLILGIATSKFLL 725
S+LKNW+VNAFRERRALALTLNDTKTAVNKLH M++ L +++I+VIWL++L IATSK+LL
Sbjct: 665 SALKNWLVNAFRERRALALTLNDTKTAVNKLHHMISFLTAIVIIVIWLILLEIATSKYLL 724
Query: 726 FVTSQLVLVAFVFGNTCKTVFESIIFLFVMHPFDVGDRCEIDGVQMIVEEMNILTTIFLR 785
F+TSQ+VL+AF+FGN+ KTVFESIIFLF++HP+DVGDR ID V+M+VEEMNILTT+FLR
Sbjct: 725 FLTSQVVLLAFMFGNSLKTVFESIIFLFIIHPYDVGDRLLIDTVEMVVEEMNILTTVFLR 784
Query: 786 YDNQKIIFPNSVLATKAIHNFYRSPDMGDGIEFCLHISTPPEKIAIMRQRIISYIEGKKE 845
DN KI++PN +L KAIHN+ RSPDMGD + C+HI+TPPEKIA ++QRI SYI+ K E
Sbjct: 785 ADNLKIVYPNILLWQKAIHNYNRSPDMGDEVTCCVHITTPPEKIAAIKQRISSYIDSKPE 832
Query: 846 HWCPAPMIVLKDVEELNRMRIAIWLTHRMNHQDMGERWTRRALLVEELVKIFQELDLQYR 905
+W P +++KDVE+LN +RIAIWL H++NHQ+MGER+TRRALL+EE++KI ELD+QYR
Sbjct: 845 YWYPKADVIVKDVEDLNIVRIAIWLCHKINHQNMGERFTRRALLIEEVIKILLELDIQYR 832
Query: 906 LLPLDINVRSLPPVNSTNLP 922
PLDINV+++P V S+ +P
Sbjct: 905 FHPLDINVKTMPTVVSSRVP 832
BLAST of Cucsa.119450 vs. TAIR10
Match:
AT2G17010.1 (AT2G17010.1 Mechanosensitive ion channel family protein)
HSP 1 Score: 519.6 bits (1337), Expect = 3.9e-147
Identity = 245/371 (66.04%), Postives = 322/371 (86.79%), Query Frame = 1
Query: 551 DEGITIDHLHKLSPKNVSAWNMKRLMNIVRHGTLSTLDEQIKDTAHEDESTTEIKSEYEA 610
D+ + + +++ KN+SAWNMKRLM IVR+ +L+TLDEQ+ ++ +EDEST +I+SE EA
Sbjct: 400 DKKVQRETRSRMNHKNISAWNMKRLMKIVRNVSLTTLDEQMLESTYEDESTRQIRSEKEA 459
Query: 611 KVAAKKIFLNVARNGSKYIYLEDLMRFMEKDEASKTMGLFEGACESRKISKSSLKNWVVN 670
K AA+KIF NV + G+KYIYLEDLMRF+ +DEA KTMGLFEGA E+++ISKS+LKNW+VN
Sbjct: 460 KAAARKIFKNVEQRGAKYIYLEDLMRFLREDEAMKTMGLFEGAPENKRISKSALKNWLVN 519
Query: 671 AFRERRALALTLNDTKTAVNKLHRMVNILVSVIILVIWLLILGIATSKFLLFVTSQLVLV 730
AFRERRALALTLNDTKTAVNKLH M+NI+ +++I+VIWL++L IA+SK LLFV+SQ+VL+
Sbjct: 520 AFRERRALALTLNDTKTAVNKLHHMINIVTAIVIVVIWLVLLEIASSKVLLFVSSQVVLL 579
Query: 731 AFVFGNTCKTVFESIIFLFVMHPFDVGDRCEIDGVQMIVEEMNILTTIFLRYDNQKIIFP 790
AF+FGNT KTVFESIIFLF++HP+DVGDRCEID VQ++VEEMNILTT+FLRYDN KI++P
Sbjct: 580 AFIFGNTVKTVFESIIFLFIVHPYDVGDRCEIDSVQLVVEEMNILTTVFLRYDNLKIMYP 639
Query: 791 NSVLATKAIHNFYRSPDMGDGIEFCLHISTPPEKIAIMRQRIISYIEGKKEHWCPAPMIV 850
NS+L K+I+N+YRSPDMGD IEFC+HI+TP EKI++++QRI +YI+ K E+W P I+
Sbjct: 640 NSLLWQKSINNYYRSPDMGDAIEFCVHITTPLEKISVIKQRISNYIDNKPEYWYPQAKII 699
Query: 851 LKDVEELNRMRIAIWLTHRMNHQDMGERWTRRALLVEELVKIFQELDLQYRLLPLDINVR 910
+KD+E+L+ +R+AIW HR+NHQDM ERWTRRA+LVEE++KI ELD+Q+R PLDINVR
Sbjct: 700 VKDLEDLHIVRLAIWPCHRINHQDMAERWTRRAVLVEEVIKILLELDIQHRFYPLDINVR 759
Query: 911 SLPPVNSTNLP 922
++P V S+ +P
Sbjct: 760 TMPTVVSSRVP 770
HSP 2 Score: 308.5 bits (789), Expect = 1.4e-83
Identity = 203/500 (40.60%), Postives = 277/500 (55.40%), Query Frame = 1
Query: 1 MDFSLKSFKSHPSYKYVRKLSGADATQDHEHLPILSDHQ--HSIMAVSDSSDRKEVIVKV 60
MDF SFKSH SYK +R G + EHLPIL DH HS M V D K
Sbjct: 1 MDFR-NSFKSHSSYKQIRS-PGDQSEPSPEHLPILHDHHPDHSGMVVDDQ--------KP 60
Query: 61 DEADASTLRATDLVNG-SGTIWRESSYDFWNDSDNRRNYGEGGAKTTD------------ 120
D +S L +G + + R++SY FW D+ + +T+D
Sbjct: 61 DSTRSS------LDDGRNAPVERDASYKFWQDNTTGTSTDHTAVRTSDKDPIAISRKGDR 120
Query: 121 ---DFEFRQHRKDVEDPPSKLI-GQFLHKQ---KASGEMSLDMDMEMLELPQDKTPLSTV 180
F+F + V++ P+K++ G+ +++Q + + E++LD+D E ++ P T
Sbjct: 121 LSGSFDFVHGKLPVDESPTKMVAGEPVNRQWRGRNNEEITLDVDQENDDVSHQTMPTPTS 180
Query: 181 AESPMRRSSRELKVSFE-----------------SISEISENDSMRRRHRDSPLDEEHRG 240
+SRE++VSF S S S + +MR L EE
Sbjct: 181 TARTSFDASREMRVSFNVRRAGGAFVAGSVPSSSSHSSSSSSATMRTNQDQPQLQEE--- 240
Query: 241 QQPRQCDRRAHGSNGEDDGAAEVLRCSSNSFFQRDVSFQRKSSLL-RAKTKSRLLDPP-E 300
EV+RC+SN +SFQRKS L+ R KT+SRL DPP E
Sbjct: 241 ---------------------EVVRCTSN------MSFQRKSELISRVKTRSRLQDPPRE 300
Query: 301 HQDRRSGRVPKSGQVRSGLISKALDEEDDDPFLEEDLPDEYKKANLGVLTLLQWASLILI 360
+ SG +SGQ++SGL++ +E+DDP EED+PDEYK+ L +TLLQW SL+ I
Sbjct: 301 EETPYSGW--RSGQLKSGLLADI--DEEDDPLAEEDVPDEYKRGKLDAITLLQWLSLVAI 360
Query: 361 IAALVCTLTIRYWRRKKLWKLEVWKWEVMILVLICGRLVSGWGIRVIVFFIERNFLLRKR 420
IAAL C+L+I+ W++ ++W L +WKWEV +LVLICGRLVSGWGIR++VFFIERNFLLRKR
Sbjct: 361 IAALACSLSIQSWKKVRVWNLHLWKWEVFLLVLICGRLVSGWGIRIVVFFIERNFLLRKR 420
Query: 421 VLYFVYGVRKAVQNCLWLGLVLIAWNFLFDDKVQREVKSNALEYVTKVLVCLLVSTLVWL 460
VLYFVYGVR+AVQNCLWLGLVL+AW+FLFD KVQRE +S K + + L+ +
Sbjct: 421 VLYFVYGVRRAVQNCLWLGLVLLAWHFLFDKKVQRETRSRMNH---KNISAWNMKRLMKI 447
BLAST of Cucsa.119450 vs. NCBI nr
Match:
gi|449444568|ref|XP_004140046.1| (PREDICTED: mechanosensitive ion channel protein 6-like [Cucumis sativus])
HSP 1 Score: 1834.3 bits (4750), Expect = 0.0e+00
Identity = 923/923 (100.00%), Postives = 923/923 (100.00%), Query Frame = 1
Query: 1 MDFSLKSFKSHPSYKYVRKLSGADATQDHEHLPILSDHQHSIMAVSDSSDRKEVIVKVDE 60
MDFSLKSFKSHPSYKYVRKLSGADATQDHEHLPILSDHQHSIMAVSDSSDRKEVIVKVDE
Sbjct: 1 MDFSLKSFKSHPSYKYVRKLSGADATQDHEHLPILSDHQHSIMAVSDSSDRKEVIVKVDE 60
Query: 61 ADASTLRATDLVNGSGTIWRESSYDFWNDSDNRRNYGEGGAKTTDDFEFRQHRKDVEDPP 120
ADASTLRATDLVNGSGTIWRESSYDFWNDSDNRRNYGEGGAKTTDDFEFRQHRKDVEDPP
Sbjct: 61 ADASTLRATDLVNGSGTIWRESSYDFWNDSDNRRNYGEGGAKTTDDFEFRQHRKDVEDPP 120
Query: 121 SKLIGQFLHKQKASGEMSLDMDMEMLELPQDKTPLSTVAESPMRRSSRELKVSFESISEI 180
SKLIGQFLHKQKASGEMSLDMDMEMLELPQDKTPLSTVAESPMRRSSRELKVSFESISEI
Sbjct: 121 SKLIGQFLHKQKASGEMSLDMDMEMLELPQDKTPLSTVAESPMRRSSRELKVSFESISEI 180
Query: 181 SENDSMRRRHRDSPLDEEHRGQQPRQCDRRAHGSNGEDDGAAEVLRCSSNSFFQRDVSFQ 240
SENDSMRRRHRDSPLDEEHRGQQPRQCDRRAHGSNGEDDGAAEVLRCSSNSFFQRDVSFQ
Sbjct: 181 SENDSMRRRHRDSPLDEEHRGQQPRQCDRRAHGSNGEDDGAAEVLRCSSNSFFQRDVSFQ 240
Query: 241 RKSSLLRAKTKSRLLDPPEHQDRRSGRVPKSGQVRSGLISKALDEEDDDPFLEEDLPDEY 300
RKSSLLRAKTKSRLLDPPEHQDRRSGRVPKSGQVRSGLISKALDEEDDDPFLEEDLPDEY
Sbjct: 241 RKSSLLRAKTKSRLLDPPEHQDRRSGRVPKSGQVRSGLISKALDEEDDDPFLEEDLPDEY 300
Query: 301 KKANLGVLTLLQWASLILIIAALVCTLTIRYWRRKKLWKLEVWKWEVMILVLICGRLVSG 360
KKANLGVLTLLQWASLILIIAALVCTLTIRYWRRKKLWKLEVWKWEVMILVLICGRLVSG
Sbjct: 301 KKANLGVLTLLQWASLILIIAALVCTLTIRYWRRKKLWKLEVWKWEVMILVLICGRLVSG 360
Query: 361 WGIRVIVFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLFDDKVQREVKSNA 420
WGIRVIVFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLFDDKVQREVKSNA
Sbjct: 361 WGIRVIVFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLFDDKVQREVKSNA 420
Query: 421 LEYVTKVLVCLLVSTLVWLVKTLMVKVLASSFHVSTYFDRIQDALFNQYVIETLSGPPLI 480
LEYVTKVLVCLLVSTLVWLVKTLMVKVLASSFHVSTYFDRIQDALFNQYVIETLSGPPLI
Sbjct: 421 LEYVTKVLVCLLVSTLVWLVKTLMVKVLASSFHVSTYFDRIQDALFNQYVIETLSGPPLI 480
Query: 481 EIQKNEEEEERLAEEVIKLQNAGATIPPDLKATAFSTAQKGGRVIGSGGLQKSPRGRSGK 540
EIQKNEEEEERLAEEVIKLQNAGATIPPDLKATAFSTAQKGGRVIGSGGLQKSPRGRSGK
Sbjct: 481 EIQKNEEEEERLAEEVIKLQNAGATIPPDLKATAFSTAQKGGRVIGSGGLQKSPRGRSGK 540
Query: 541 LSRTLSKKGGDEGITIDHLHKLSPKNVSAWNMKRLMNIVRHGTLSTLDEQIKDTAHEDES 600
LSRTLSKKGGDEGITIDHLHKLSPKNVSAWNMKRLMNIVRHGTLSTLDEQIKDTAHEDES
Sbjct: 541 LSRTLSKKGGDEGITIDHLHKLSPKNVSAWNMKRLMNIVRHGTLSTLDEQIKDTAHEDES 600
Query: 601 TTEIKSEYEAKVAAKKIFLNVARNGSKYIYLEDLMRFMEKDEASKTMGLFEGACESRKIS 660
TTEIKSEYEAKVAAKKIFLNVARNGSKYIYLEDLMRFMEKDEASKTMGLFEGACESRKIS
Sbjct: 601 TTEIKSEYEAKVAAKKIFLNVARNGSKYIYLEDLMRFMEKDEASKTMGLFEGACESRKIS 660
Query: 661 KSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVIILVIWLLILGIATSKFL 720
KSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVIILVIWLLILGIATSKFL
Sbjct: 661 KSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVIILVIWLLILGIATSKFL 720
Query: 721 LFVTSQLVLVAFVFGNTCKTVFESIIFLFVMHPFDVGDRCEIDGVQMIVEEMNILTTIFL 780
LFVTSQLVLVAFVFGNTCKTVFESIIFLFVMHPFDVGDRCEIDGVQMIVEEMNILTTIFL
Sbjct: 721 LFVTSQLVLVAFVFGNTCKTVFESIIFLFVMHPFDVGDRCEIDGVQMIVEEMNILTTIFL 780
Query: 781 RYDNQKIIFPNSVLATKAIHNFYRSPDMGDGIEFCLHISTPPEKIAIMRQRIISYIEGKK 840
RYDNQKIIFPNSVLATKAIHNFYRSPDMGDGIEFCLHISTPPEKIAIMRQRIISYIEGKK
Sbjct: 781 RYDNQKIIFPNSVLATKAIHNFYRSPDMGDGIEFCLHISTPPEKIAIMRQRIISYIEGKK 840
Query: 841 EHWCPAPMIVLKDVEELNRMRIAIWLTHRMNHQDMGERWTRRALLVEELVKIFQELDLQY 900
EHWCPAPMIVLKDVEELNRMRIAIWLTHRMNHQDMGERWTRRALLVEELVKIFQELDLQY
Sbjct: 841 EHWCPAPMIVLKDVEELNRMRIAIWLTHRMNHQDMGERWTRRALLVEELVKIFQELDLQY 900
Query: 901 RLLPLDINVRSLPPVNSTNLPQR 924
RLLPLDINVRSLPPVNSTNLPQR
Sbjct: 901 RLLPLDINVRSLPPVNSTNLPQR 923
BLAST of Cucsa.119450 vs. NCBI nr
Match:
gi|659095000|ref|XP_008448342.1| (PREDICTED: mechanosensitive ion channel protein 6-like [Cucumis melo])
HSP 1 Score: 1808.1 bits (4682), Expect = 0.0e+00
Identity = 907/923 (98.27%), Postives = 916/923 (99.24%), Query Frame = 1
Query: 1 MDFSLKSFKSHPSYKYVRKLSGADATQDHEHLPILSDHQHSIMAVSDSSDRKEVIVKVDE 60
MDFSLKSFKSHPSYKYVRKLSGADATQD EHLPILSDHQHSIMAVSD+SDRKEVIVKVDE
Sbjct: 1 MDFSLKSFKSHPSYKYVRKLSGADATQDQEHLPILSDHQHSIMAVSDASDRKEVIVKVDE 60
Query: 61 ADASTLRATDLVNGSGTIWRESSYDFWNDSDNRRNYGEGGAKTTDDFEFRQHRKDVEDPP 120
ADAS+LRATDLVNGSG IWRESSYDFWNDSDNRRN GEGGA+TT+DFEFRQHRKDVEDPP
Sbjct: 61 ADASSLRATDLVNGSGRIWRESSYDFWNDSDNRRNNGEGGARTTEDFEFRQHRKDVEDPP 120
Query: 121 SKLIGQFLHKQKASGEMSLDMDMEMLELPQDKTPLSTVAESPMRRSSRELKVSFESISEI 180
SKLIGQFLHKQKASGEMSLDMDMEMLELPQDKTPLSTVAESPMRRSSRELKVSFESISEI
Sbjct: 121 SKLIGQFLHKQKASGEMSLDMDMEMLELPQDKTPLSTVAESPMRRSSRELKVSFESISEI 180
Query: 181 SENDSMRRRHRDSPLDEEHRGQQPRQCDRRAHGSNGEDDGAAEVLRCSSNSFFQRDVSFQ 240
SENDSMRRRHRDSPLDEEHRGQQPRQCDRRAHGSNGEDDGAAEVLRCSSNS FQRDVSFQ
Sbjct: 181 SENDSMRRRHRDSPLDEEHRGQQPRQCDRRAHGSNGEDDGAAEVLRCSSNSSFQRDVSFQ 240
Query: 241 RKSSLLRAKTKSRLLDPPEHQDRRSGRVPKSGQVRSGLISKALDEEDDDPFLEEDLPDEY 300
RKSSLLRAKTKSRLLDPPEHQDRRSGRVPKSGQVRSGLISK LDEEDDDPFLEEDLPDEY
Sbjct: 241 RKSSLLRAKTKSRLLDPPEHQDRRSGRVPKSGQVRSGLISKVLDEEDDDPFLEEDLPDEY 300
Query: 301 KKANLGVLTLLQWASLILIIAALVCTLTIRYWRRKKLWKLEVWKWEVMILVLICGRLVSG 360
KKANLGVLTLLQWASLILIIAALVCTLTIRYWRRKKLWKLEVWKWEVMILVLICGRLVSG
Sbjct: 301 KKANLGVLTLLQWASLILIIAALVCTLTIRYWRRKKLWKLEVWKWEVMILVLICGRLVSG 360
Query: 361 WGIRVIVFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLFDDKVQREVKSNA 420
WGIRVIVFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLFDDKVQREVKSNA
Sbjct: 361 WGIRVIVFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLFDDKVQREVKSNA 420
Query: 421 LEYVTKVLVCLLVSTLVWLVKTLMVKVLASSFHVSTYFDRIQDALFNQYVIETLSGPPLI 480
LEYVTKVLVCLL+STLVWLVKTLMVKVLASSFHVSTYFDRIQDALFNQYVIETLSGPPLI
Sbjct: 421 LEYVTKVLVCLLISTLVWLVKTLMVKVLASSFHVSTYFDRIQDALFNQYVIETLSGPPLI 480
Query: 481 EIQKNEEEEERLAEEVIKLQNAGATIPPDLKATAFSTAQKGGRVIGSGGLQKSPRGRSGK 540
EIQKNEEEEERLAEEV+KLQNAGATIPPDLKATAFSTAQKGGRVIGSGGLQKSPRGRSGK
Sbjct: 481 EIQKNEEEEERLAEEVMKLQNAGATIPPDLKATAFSTAQKGGRVIGSGGLQKSPRGRSGK 540
Query: 541 LSRTLSKKGGDEGITIDHLHKLSPKNVSAWNMKRLMNIVRHGTLSTLDEQIKDTAHEDES 600
LSRTLSKKGGDEGITIDHLHKLSPKNVSAWNMKRLMNIVRHGTLSTLDEQIKDTAHEDES
Sbjct: 541 LSRTLSKKGGDEGITIDHLHKLSPKNVSAWNMKRLMNIVRHGTLSTLDEQIKDTAHEDES 600
Query: 601 TTEIKSEYEAKVAAKKIFLNVARNGSKYIYLEDLMRFMEKDEASKTMGLFEGACESRKIS 660
TT+IKSEYEAKVAAKKIFLNVARNGSKYIYLEDLMRFMEKDEASKTMGLFEGACESRKIS
Sbjct: 601 TTQIKSEYEAKVAAKKIFLNVARNGSKYIYLEDLMRFMEKDEASKTMGLFEGACESRKIS 660
Query: 661 KSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVIILVIWLLILGIATSKFL 720
KSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVIILVIWLLILGIATSKFL
Sbjct: 661 KSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVIILVIWLLILGIATSKFL 720
Query: 721 LFVTSQLVLVAFVFGNTCKTVFESIIFLFVMHPFDVGDRCEIDGVQMIVEEMNILTTIFL 780
LFVTSQLVLVAFVFGNTCKTVFESIIFLFVMHPFDVGDRCEIDGVQMIVEEMNILTTIFL
Sbjct: 721 LFVTSQLVLVAFVFGNTCKTVFESIIFLFVMHPFDVGDRCEIDGVQMIVEEMNILTTIFL 780
Query: 781 RYDNQKIIFPNSVLATKAIHNFYRSPDMGDGIEFCLHISTPPEKIAIMRQRIISYIEGKK 840
RYDNQKIIFPNSVLATKAIHNFYRSPDMGDG+EFCLHISTPPEKIAIMRQRI+SYIEGKK
Sbjct: 781 RYDNQKIIFPNSVLATKAIHNFYRSPDMGDGVEFCLHISTPPEKIAIMRQRIVSYIEGKK 840
Query: 841 EHWCPAPMIVLKDVEELNRMRIAIWLTHRMNHQDMGERWTRRALLVEELVKIFQELDLQY 900
EHWCPAPMIVLKDVEELNRMRIAIWLTHRMNHQDMGERWTRRALLVEELVKIFQELDLQY
Sbjct: 841 EHWCPAPMIVLKDVEELNRMRIAIWLTHRMNHQDMGERWTRRALLVEELVKIFQELDLQY 900
Query: 901 RLLPLDINVRSLPPVNSTNLPQR 924
RLLPLDINVRSLPPVNSTN P R
Sbjct: 901 RLLPLDINVRSLPPVNSTNFPPR 923
BLAST of Cucsa.119450 vs. NCBI nr
Match:
gi|703062965|ref|XP_010086848.1| (Mechanosensitive ion channel protein 8 [Morus notabilis])
HSP 1 Score: 1224.9 bits (3168), Expect = 0.0e+00
Identity = 653/966 (67.60%), Postives = 765/966 (79.19%), Query Frame = 1
Query: 1 MDFSLK-SFKSHPSYKYVRKLS--GADATQDHEHLPILSDHQ----HSIM---AVSD--- 60
MDFSLK SFKSH SYK RK+S G + HE LPILSDH H + ++D
Sbjct: 1 MDFSLKKSFKSHGSYKNSRKISAGGGNPDNSHEELPILSDHDFDDNHHVQHYRRLNDNGK 60
Query: 61 ----SSDRKEVIVKVDEADASTLRATDLVNGSGTIWRESSYDFWNDSDNRRNYGEGGAKT 120
S+ R EVIVK+DE A+D + WRE+SY W ++ N R+ G G
Sbjct: 61 MGGASTGRNEVIVKIDEGGNPPAAASDQSRNAENFWRENSYGAWKENANVRDGGGSG--- 120
Query: 121 TDDFEFRQHRKDVEDPPSKLIGQFLHKQKASGEMSLDMDMEMLELPQD-----KTPLSTV 180
+ F F Q EDPPSKLI QFLH+QKASG++SLDMD+EM EL + + L+ V
Sbjct: 121 -ESFHFAQ---TTEDPPSKLIRQFLHRQKASGDISLDMDLEMEELRRSAGSGGELDLTPV 180
Query: 181 AESPMR-RSSRELKVSFESI-----SEISENDSMRRRHRDSPLDEEHRGQQPRQCDRRAH 240
+ESP RSSRELKVS SEIS+ND++RRR +DSP D + +RR
Sbjct: 181 SESPQNSRSSRELKVSLHPSPSDVGSEISQNDAVRRRFKDSPNDNDE------SLERR-- 240
Query: 241 GSNGEDDGAAEVLRCSSNSFFQRDVSFQRKSSLLRAKTKSRLLDPPEHQDRRSGRVPKSG 300
E DG EV+RC+SN+ FQR++SFQR+SS LR KT+SRL DPPE D RSGR+PKSG
Sbjct: 241 ----ERDGE-EVVRCTSNASFQREISFQRRSSFLRVKTRSRLQDPPEEPDMRSGRIPKSG 300
Query: 301 QV------------RSGLISKALDEEDDDPFLEEDLPDEYKKANLGVLTLLQWASLILII 360
Q+ +SGL+ K DEE+DDPF +EDLPDE+KKANL LTLLQWASLILI+
Sbjct: 301 QIPRSGPIPRSGPMKSGLLGK--DEEEDDPFSDEDLPDEFKKANLSALTLLQWASLILIV 360
Query: 361 AALVCTLTIRYWRRKKLWKLEVWKWEVMILVLICGRLVSGWGIRVIVFFIERNFLLRKRV 420
CTL+I Y R K L KL++WKWEV+ILVLICGRLVSGWGIR+ VFF ERNFLLRKRV
Sbjct: 361 GLFACTLSIHYLRHKNLLKLKLWKWEVLILVLICGRLVSGWGIRIAVFFFERNFLLRKRV 420
Query: 421 LYFVYGVRKAVQNCLWLGLVLIAWNFLFDDKVQREVKSNALEYVTKVLVCLLVSTLVWLV 480
LYFVYGVRKAVQNCLWLGLVLIAW+FLFD +V+RE +S +L+YVTKVL+C LV TL+WLV
Sbjct: 421 LYFVYGVRKAVQNCLWLGLVLIAWHFLFDKRVERETRSESLKYVTKVLICFLVGTLLWLV 480
Query: 481 KTLMVKVLASSFHVSTYFDRIQDALFNQYVIETLSGPPLIEIQKNEEEEERLAEEVIKLQ 540
KTL+VKVLASSFHVSTYFDRIQD+LFNQ+VIETLSGPPLIEI K EEEEERLA+EV KLQ
Sbjct: 481 KTLIVKVLASSFHVSTYFDRIQDSLFNQFVIETLSGPPLIEIYKTEEEEERLADEVEKLQ 540
Query: 541 NAGATIPPDLKATAFSTA-QKGGRVIGSGGLQKSPRGRSGKLSRTLSKKGGDEGITIDHL 600
NAGATIPPDL AFS+A + RVIGSG L ++ +S KLSR+LSK G DEGITIDHL
Sbjct: 541 NAGATIPPDL---AFSSALPRTSRVIGSGRLSRT--WKSSKLSRSLSKIG-DEGITIDHL 600
Query: 601 HKLSPKNVSAWNMKRLMNIVRHGTLSTLDEQIKDTAHEDESTTEIKSEYEAKVAAKKIFL 660
HKL+PKNVSAWNMKRLM +VRHGTL+TLDEQI D+ HEDES T+I+SE EAK AAKKIF
Sbjct: 601 HKLNPKNVSAWNMKRLMRMVRHGTLTTLDEQIVDSTHEDESATQIRSEVEAKAAAKKIFQ 660
Query: 661 NVARNGSKYIYLEDLMRFMEKDEASKTMGLFEGACESRKISKSSLKNWVVNAFRERRALA 720
NVAR GSK+I LEDLMRFM +DEA KTM LFEGA ES++ISKSSLKNWVVNAFRERRALA
Sbjct: 661 NVARRGSKFICLEDLMRFMREDEAVKTMSLFEGASESQRISKSSLKNWVVNAFRERRALA 720
Query: 721 LTLNDTKTAVNKLHRMVNILVSVIILVIWLLILGIATSKFLLFVTSQLVLVAFVFGNTCK 780
LTLNDTKTAVNKLHR+VN++V+++I VIWLLILGIATSKFLLF++SQLVLVAF+FGNTCK
Sbjct: 721 LTLNDTKTAVNKLHRIVNVIVAIVIGVIWLLILGIATSKFLLFISSQLVLVAFIFGNTCK 780
Query: 781 TVFESIIFLFVMHPFDVGDRCEIDGVQMIVEEMNILTTIFLRYDNQKIIFPNSVLATKAI 840
TVFE+I+FLFVMHPFDVGDRCEIDGVQM+VEEMNILTT+FLRYDN KIIFPNSVLATKAI
Sbjct: 781 TVFEAIVFLFVMHPFDVGDRCEIDGVQMVVEEMNILTTVFLRYDNAKIIFPNSVLATKAI 840
Query: 841 HNFYRSPDMGDGIEFCLHISTPPEKIAIMRQRIISYIEGKKEHWCPAPMIVLKDVEELNR 900
NFYRSPDMGDGIEF +H++TP +KIA MRQRI +Y+E KKEHW PAPM+++KD++ELNR
Sbjct: 841 DNFYRSPDMGDGIEFSIHVATPADKIAAMRQRITNYVENKKEHWYPAPMVIMKDLDELNR 900
Query: 901 MRIAIWLTHRMNHQDMGERWTRRALLVEELVKIFQELDLQYRLLPLDINVRSLPPV---- 922
+R+A+WL HR+NHQDMGER+ RR+LL+EE+VKIFQELD+QYRLLP+DINVR++P V
Sbjct: 901 VRMAVWLCHRINHQDMGERYARRSLLIEEMVKIFQELDIQYRLLPIDINVRAMPSVAPVP 938
BLAST of Cucsa.119450 vs. NCBI nr
Match:
gi|1009150892|ref|XP_015893266.1| (PREDICTED: mechanosensitive ion channel protein 6-like [Ziziphus jujuba])
HSP 1 Score: 1224.5 bits (3167), Expect = 0.0e+00
Identity = 641/968 (66.22%), Postives = 753/968 (77.79%), Query Frame = 1
Query: 1 MDFSLK-SFKSHPSYKYVRKLSGADATQDHEH-----LPILSDH----------QH---- 60
MDFSLK SFKSH SYK + K+ G H H LPIL D QH
Sbjct: 1 MDFSLKKSFKSHGSYKQMLKIGGGIGNDHHSHDDPEGLPILFDQDVAVAEHHPQQHLDSM 60
Query: 61 -SIMAVSDSSDRKEVIVKVDEADASTLRAT--DLVNGSGTIWRESSYDFWNDSDNRRNYG 120
S DS++R+EVIVK+D+ D+ST IWR SSY+FW + DN G
Sbjct: 61 SSAAVAGDSNNRREVIVKIDDGDSSTSSRNMEQQQQQQSKIWRGSSYEFWKEDDNNVRDG 120
Query: 121 EGGAKTTDDFEFRQHRKDV------EDPPSKLIGQFLHKQKASGEMSLDMDMEMLELPQD 180
DDF F Q + +DPPSKLIGQFLHKQKASG+MSLDMD+EM EL +
Sbjct: 121 N-----KDDFRFVQRGQSSSASASPDDPPSKLIGQFLHKQKASGDMSLDMDLEMDELRHE 180
Query: 181 KTPLSTVAESPMRRSS----RELKVSFESISEISENDSMRRRHRDSPLDEEHRGQQPRQC 240
+ L +AESP +RSS +ELKVSF+ + + ND + R L E R +
Sbjct: 181 RN-LPPLAESPAKRSSFGQSKELKVSFQPTTTAASNDDVEIR-----LSESVRRRNKDST 240
Query: 241 DRRAHGSNGEDDGAAEVLRCSSNSFFQRDVSFQRKSSLLRAK-TKSRLLDPPEHQDRRSG 300
+ + G +G +G EV+RC+SN+ F+R+VSFQ KSSLLR K TKSRL+DPPE + R+
Sbjct: 241 EEDSSGGSGRGNGDDEVVRCTSNAAFEREVSFQSKSSLLRLKKTKSRLIDPPEEPENRAS 300
Query: 301 RVPKSGQV------------RSGLISK-ALDEEDDDPFLEEDLPDEYKKANLGVLTLLQW 360
RVPKSGQV RSG++ LD++DDDPF E+DLPD+YKKANL LTLLQW
Sbjct: 301 RVPKSGQVMKSGQMLKSGQLRSGMLGNWPLDDDDDDPFWEDDLPDDYKKANLSALTLLQW 360
Query: 361 ASLILIIAALVCTLTIRYWRRKKLWKLEVWKWEVMILVLICGRLVSGWGIRVIVFFIERN 420
SLI+II CTL+I + RRK WKL++WKWEV++LVLICGRL SGWG+R++VFFIERN
Sbjct: 361 LSLIVIIGVFACTLSIPFLRRKNWWKLKLWKWEVLVLVLICGRLFSGWGVRIVVFFIERN 420
Query: 421 FLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLFDDKVQREVKSNALEYVTKVLVCLLV 480
FLLRKRVLYFVYG+RKAVQNCLWLGLVL+AW+FLFD+KV+RE KS+ L+YVTKVLVCLLV
Sbjct: 421 FLLRKRVLYFVYGLRKAVQNCLWLGLVLLAWHFLFDEKVERETKSDKLKYVTKVLVCLLV 480
Query: 481 STLVWLVKTLMVKVLASSFHVSTYFDRIQDALFNQYVIETLSGPPLIEIQKNEEEEERLA 540
TLVWLVKTL+VKVLASSFHVS YFDRIQ++LFNQYVIETLSGPPLIE+++ EEE+ERL
Sbjct: 481 GTLVWLVKTLIVKVLASSFHVSKYFDRIQESLFNQYVIETLSGPPLIEMKRTEEEDERLV 540
Query: 541 EEVIKLQNAGATIPPDLKATAFSTAQKGGRVIGSGGLQKSPRGRSGKLSRTLSKKGGDEG 600
+EV KLQNAGATIPPDLK AF TA K GRVIGSGGLQKSPR RS K S+ LSKK D+G
Sbjct: 541 DEVRKLQNAGATIPPDLKNAAFPTA-KSGRVIGSGGLQKSPR-RSNKFSQPLSKKQ-DDG 600
Query: 601 ITIDHLHKLSPKNVSAWNMKRLMNIVRHGTLSTLDEQIKDTAHEDESTTEIKSEYEAKVA 660
ITIDHLHKL+PKNVSAWNMKRLMNIVRHG+L+TLDEQI+D+ +DE T+IKSE EAK A
Sbjct: 601 ITIDHLHKLNPKNVSAWNMKRLMNIVRHGSLTTLDEQIQDSTRDDEKATQIKSEVEAKAA 660
Query: 661 AKKIFLNVARNGSKYIYLEDLMRFMEKDEASKTMGLFEGACESRKISKSSLKNWVVNAFR 720
AKKIF NVAR+GSK+IYL DL RF+ DEA KTM LFEGA ES KISK+SLKNWVVNAFR
Sbjct: 661 AKKIFQNVARHGSKHIYLRDLTRFLRDDEALKTMSLFEGASESGKISKTSLKNWVVNAFR 720
Query: 721 ERRALALTLNDTKTAVNKLHRMVNILVSVIILVIWLLILGIATSKFLLFVTSQLVLVAFV 780
ERRALALTLNDTKTAVNKLH MV I++ +I VIWLLILGIAT+KFLLFV+SQLVLVAF+
Sbjct: 721 ERRALALTLNDTKTAVNKLHHMVKIIIIFVIGVIWLLILGIATTKFLLFVSSQLVLVAFI 780
Query: 781 FGNTCKTVFESIIFLFVMHPFDVGDRCEIDGVQMIVEEMNILTTIFLRYDNQKIIFPNSV 840
FGNTCKTVFE+IIFLFV+HPFDVGDRCEIDGVQM+VEEMNILTT+FLRYDN KI+FPNSV
Sbjct: 781 FGNTCKTVFEAIIFLFVVHPFDVGDRCEIDGVQMVVEEMNILTTVFLRYDNTKIVFPNSV 840
Query: 841 LATKAIHNFYRSPDMGDGIEFCLHISTPPEKIAIMRQRIISYIEGKKEHWCPAPMIVLKD 900
L+TK I+N+YRSPDMGD +EFC+H+STP E+IA ++ RI SYIE KKEHWC PMIV+KD
Sbjct: 841 LSTKPINNYYRSPDMGDAVEFCIHVSTPAEEIAAIKHRITSYIENKKEHWCTQPMIVMKD 900
Query: 901 VEELNRMRIAIWLTHRMNHQDMGERWTRRALLVEELVKIFQELDLQYRLLPLDINVRSLP 922
VEELNR+R A+WL H+MN QD+GERW RR+LLVEE++KIF+ELDLQYRL PLDIN+ S+P
Sbjct: 901 VEELNRVRFAVWLNHKMNFQDIGERWMRRSLLVEEMIKIFRELDLQYRLFPLDINICSMP 954
BLAST of Cucsa.119450 vs. NCBI nr
Match:
gi|658027009|ref|XP_008348933.1| (PREDICTED: mechanosensitive ion channel protein 6-like [Malus domestica])
HSP 1 Score: 1211.4 bits (3133), Expect = 0.0e+00
Identity = 626/943 (66.38%), Postives = 751/943 (79.64%), Query Frame = 1
Query: 1 MDFSLKS-FKSHPSYKYVRKLSGADATQDHEHLPILSDHQH----SIMAVSDSSDRKEVI 60
M+FS+K FKSH S K++RK+S + HE LPIL DH H M D SDR+EVI
Sbjct: 1 MEFSIKKPFKSHGSAKHMRKISAGEEDISHEELPILLDHDHRHHTQPMTDVDLSDRREVI 60
Query: 61 VKVDEADASTLRATDLVNGSGTIWRESSYDFWNDSDNRRNYGEGGAKTTDDFEFRQHRKD 120
VK+DE S+ +D IWR+SS DFW+ N GA ++F+F Q K
Sbjct: 61 VKIDEGADSSTSTSDPAKNGRKIWRQSSIDFWHGDGN-------GAGNAENFDFVQRGKT 120
Query: 121 VE----DPPSKLIGQFLHKQKASGEMSLDMDMEMLELPQDKTPLSTVAESPMRRSSR--- 180
E DPPSKLIGQFLHKQ+ASG+M+LDMD+EM EL Q++ L +AESP R+SR
Sbjct: 121 EEGTGEDPPSKLIGQFLHKQRASGDMTLDMDLEMEELQQNERDLPPLAESPSPRNSRNSK 180
Query: 181 ELKVSFES----ISEISENDSMRRRHRDSPLDEEHRGQQPRQCDRRAHGSNGEDDGAAEV 240
ELKVSF+S +++ + +S+RRR+R+SP DEE R R SNG+DD V
Sbjct: 181 ELKVSFQSPASDLTDTPQTESVRRRYRESP-DEERR--------RTERLSNGQDD----V 240
Query: 241 LRCSSNSFFQRDVSFQRKSSLLRAKTKSRLLDPPEHQDRRSGRVP------KSGQVRSGL 300
+RC+SN+ F+R+ SF KS LLR KTKSRL+DPPE D +SGR+P KSGQ+RSGL
Sbjct: 241 VRCTSNASFRREPSFSNKSDLLRIKTKSRLIDPPEEPDFKSGRIPRSGQIPKSGQMRSGL 300
Query: 301 ISKALDEEDDD-PFLEEDLPDEYKKANLGVLTLLQWASLILIIAALVCTLTIRYWRRKKL 360
+S+ D++DDD PFLEED+P EYK+AN LTLLQW SL+LII A+VCTLTI R K L
Sbjct: 301 LSRGGDDDDDDDPFLEEDVPHEYKRANFNALTLLQWVSLVLIIGAVVCTLTIPVLREKSL 360
Query: 361 WKLEVWKWEVMILVLICGRLVSGWGIRVIVFFIERNFLLRKRVLYFVYGVRKAVQNCLWL 420
WKL++WKWEV+ILVLICGRLVSGWGIR+IVFF+ERNFLLRKRVLYFVYGVRKAVQNCLWL
Sbjct: 361 WKLKLWKWEVLILVLICGRLVSGWGIRIIVFFVERNFLLRKRVLYFVYGVRKAVQNCLWL 420
Query: 421 GLVLIAWNFLFDDKVQREVKSNALEYVTKVLVCLLVSTLVWLVKTLMVKVLASSFHVSTY 480
GLVL+AW+F+FD KV+RE + + YVTK+L CLL+ L+WLVKTL+VKVLASSFHV +Y
Sbjct: 421 GLVLLAWHFMFDKKVERETNTEVVAYVTKILFCLLIGVLLWLVKTLIVKVLASSFHVRSY 480
Query: 481 FDRIQDALFNQYVIETLSGPPLIEIQKNEEEEERLAEEVIKLQNAGATIPPDLKATAFST 540
FDRIQD+LFNQYVIETLSG PLIE+Q EEEE+RLA+EV KLQNAGAT+PPDLKA AF +
Sbjct: 481 FDRIQDSLFNQYVIETLSGRPLIEMQNAEEEEDRLADEVRKLQNAGATMPPDLKANAFPS 540
Query: 541 AQKGGRVIGSGGLQKSPR-------GRSGKLSRTLSKKGGDEGITIDHLHKLSPKNVSAW 600
A+ G RVIGSG ++ G+S K SR LSK+ + GITIDHLHKL+PKNVSAW
Sbjct: 541 ARIG-RVIGSGSIRSGRVIAGSGLIGKSTKYSRPLSKRAEEPGITIDHLHKLNPKNVSAW 600
Query: 601 NMKRLMNIVRHGTLSTLDEQIKDTAHEDESTTEIKSEYEAKVAAKKIFLNVARNGSKYIY 660
NMKRLMNIVR G L+TLDEQI D EDE+ T+I+SE EAK AAKKIF NVAR GSK+IY
Sbjct: 601 NMKRLMNIVRKGHLTTLDEQILDATGEDEADTQIRSEVEAKAAAKKIFQNVARPGSKFIY 660
Query: 661 LEDLMRFMEKDEASKTMGLFEGACESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVN 720
LEDLMRFME+DEA KTM LFEGA E ++ISKS+LKNWVV+AFRERRALALTLNDTKTAVN
Sbjct: 661 LEDLMRFMEEDEAVKTMSLFEGASEHKRISKSALKNWVVSAFRERRALALTLNDTKTAVN 720
Query: 721 KLHRMVNILVSVIILVIWLLILGIATSKFLLFVTSQLVLVAFVFGNTCKTVFESIIFLFV 780
LHR+VNI+V+++I+VIWLL+LGIATSKFLLFV+SQLV+VAFVFGNTCKT+FE+IIFLFV
Sbjct: 721 TLHRVVNIIVAIVIIVIWLLVLGIATSKFLLFVSSQLVVVAFVFGNTCKTIFEAIIFLFV 780
Query: 781 MHPFDVGDRCEIDGVQMIVEEMNILTTIFLRYDNQKIIFPNSVLATKAIHNFYRSPDMGD 840
MHPFDVGDRC+IDGVQM+VEEMNILTT+FLRYDN KI++PNS+LAT I N+YRSPD GD
Sbjct: 781 MHPFDVGDRCDIDGVQMVVEEMNILTTVFLRYDNTKIVYPNSILATLPILNYYRSPDTGD 840
Query: 841 GIEFCLHISTPPEKIAIMRQRIISYIEGKKEHWCPAPMIVLKDVEELNRMRIAIWLTHRM 900
IEFC+H+STPP++I +MRQRIISYIE KKEHW PAPM+++KDVEELNR+R AIW HRM
Sbjct: 841 TIEFCIHVSTPPDRIVLMRQRIISYIENKKEHWYPAPMVIMKDVEELNRVRFAIWPQHRM 900
Query: 901 NHQDMGERWTRRALLVEELVKIFQELDLQYRLLPLDINVRSLP 914
N QD+GER+ RR LVEE+V+IFQELD+Q+RLLP+DINVR++P
Sbjct: 901 NFQDIGERFVRRGYLVEEMVRIFQELDIQFRLLPVDINVRAMP 922
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| MSL8_ARATH | 1.1e-284 | 54.47 | Mechanosensitive ion channel protein 8 OS=Arabidopsis thaliana GN=MSL8 PE=2 SV=2 | [more] |
| MSL5_ARATH | 2.4e-284 | 56.56 | Mechanosensitive ion channel protein 5 OS=Arabidopsis thaliana GN=MSL5 PE=2 SV=1 | [more] |
| MSL4_ARATH | 2.5e-273 | 55.25 | Mechanosensitive ion channel protein 4 OS=Arabidopsis thaliana GN=MSL4 PE=3 SV=1 | [more] |
| MSL6_ARATH | 9.0e-271 | 63.86 | Mechanosensitive ion channel protein 6 OS=Arabidopsis thaliana GN=MSL6 PE=1 SV=1 | [more] |
| MSL7_ARATH | 7.9e-251 | 51.20 | Mechanosensitive ion channel protein 7 OS=Arabidopsis thaliana GN=MSL7 PE=3 SV=1 | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A0A0KE62_CUCSA | 0.0e+00 | 100.00 | Uncharacterized protein OS=Cucumis sativus GN=Csa_6G124170 PE=4 SV=1 | [more] |
| W9QBV6_9ROSA | 0.0e+00 | 67.60 | Mechanosensitive ion channel protein 8 OS=Morus notabilis GN=L484_006078 PE=4 SV... | [more] |
| M5VK71_PRUPE | 0.0e+00 | 65.18 | Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa001020mg PE=4 SV=1 | [more] |
| A0A0A0K619_CUCSA | 0.0e+00 | 64.54 | Uncharacterized protein OS=Cucumis sativus GN=Csa_7G017150 PE=4 SV=1 | [more] |
| A0A061FGA7_THECC | 0.0e+00 | 67.83 | Mechanosensitive channel of small conductance-like 6, putative OS=Theobroma caca... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| AT3G14810.1 | 1.4e-285 | 56.56 | mechanosensitive channel of small conductance-like 5 | [more] |
| AT1G53470.1 | 1.4e-274 | 55.25 | mechanosensitive channel of small conductance-like 4 | [more] |
| AT1G78610.1 | 5.1e-272 | 63.86 | mechanosensitive channel of small conductance-like 6 | [more] |
| AT2G17000.1 | 4.5e-252 | 51.20 | Mechanosensitive ion channel family protein | [more] |
| AT2G17010.1 | 3.9e-147 | 66.04 | Mechanosensitive ion channel family protein | [more] |
