Cucsa.271500 (gene) Cucumber (Gy14) v1

NameCucsa.271500
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionPhospholipase D Z
Locationscaffold02500 : 338784 .. 342792 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGAAAGCTCAAACTCCAACTTTAACTACCATTTTCAGTGCTCTCTTCTTCTTCTTCTTCGTCAGCCAATTCCCGCCTGTTCTCTCCTTCACTCAATCACAATGCAAGGGTTGGCTTGTACAATCAATTCCCACAGATATGCCGCAGCTTCAGCTGGTTCAAGGCGTCCTCTCGACTCGTAAATCTAACACTCATTTTTCCATTTGCATTTCAAATTCATTTGATTCTTTTGCTTATTTCTTTGCTCATCTGTTTAGATTCCTAGAAACTTTGTCCTAATTCTTAGATTTGTTGGAATATCAGGAGATGTATTCGTATGGTTAGCTGGAAACTCGACGCGGCGTTTGGATATCATTGCTCAGTACTGGGAATTACTAGCTGGTCCTAATGATTCTCGATCAGGGGACTATGGATACTCGGATGAGGATTTGAAGAAATTTGGGGCTAATAAAGGTTATGATGTTTATTCAGCCATAGAGAAAGCTGCCGATCGCAATGTCAATGTCAGGTACATTGAATCTGGGTTTGGTCGATGGTGTTGCTTTGATAACAGTATAGGGTTCGGAAATGGAAATGGTTTAAACCCAAATGGCAAACTGCTCTGGAGTTGACCTTACAAACTGTAGTATTAAATTACATTTGGTAGTTCAAAATATGCGTATTTTACTCCTTGAAGAGGATAGGATCAATATGTAAGTTTTAAGATTTTTAATTGCTTATCCATTTGAATGGTGAAACAATTTCTGGTTATGAACTATAAGCAGACATTTTCAAGAAGAAGATATGATATTGCCAAAATAAGCTTAACTCAACGGTATTGGTATGATCTCCATACCAAGGGGTTGGAAGTTCATTCCCCCGCTCCGTGAACTCAAAAAAaGAAAAAGAAGAAAGAAAAGAAAAAAGAAGATATGAGTAATTCTTGGGAATGCAGCTAAAGTTTCAGATCTATTAGATAGACAACTTTTGATTTGAAACGAGTTGGAAAAAACCTCCCACTTTATGGATAAGAAAAATGACCGTAGGTGAAACATAATAAATATATAATCTTGAAATTCTTTATTATTTTTATTAATTTGAGAACTTTTAATTATGTAGGCTGGTATCTCACTCGGGGGTATCTCCAAACTATGGAAAAGAACCAGCTGACTTAGCATCTGGAAGGCCAAACGTGCAGAATGTAACTTTATTACTTGGTGATTGGTATGGATCAGGCATAGTCCACTCCAAAGTTTGGATATCTGATGATAGAGATGTGTATATAGGGTCTGCAAACCAGGACTGGAAATCTCTTTCACAGGTAAGCTTTTATTGGAAGGATTTTTTATATCAAGAACTTACCTTCAATCACATTGTTTATCGATGAGGATTGAGGATGGTTTAAAACAGATGGAGCTCCAAATCTTTGATACTGGTTTTTATTTATTGGTGAAATAATTCTGACTTCCAGGGTATCCTTTTCGAACGTTTTTGTTGAATTCTTTGCTTTTCTATAGTTGGTCACACAAACACAACGAAATTGAATCTTAAGGTTGATGCTCCTATCACTTGATGGTTTTAGGTGAAGGAACTTGGAATATATCTTACAGGCTGTCCAAAAATAGCAGCTCATGTCAAGGTCTATTTTGAAAATTTATTGAAACTTGCATTTCTTAATCGCACGGATTACACAAGAACAATATTTGACCACCAGTGGCAAACACAAAGAAAAGTTCCTTGCTGGTCATATTTTATTGATCCCGAGTCTAGATGCAGGCAAGTCCAACCATTCTCGTCATCCTTTGTTTTTCAGAAGAATGTACTTTTGTGATTTATTCGGTATTGGTAGTAGATTTCTTTTTtCTTCCTTTTTTtCTGAGTTGTTGGATTTAGGTAACTGAAGACCTCTTTTTCTAGCTCCTGAAGGAATGCATACATCACGAGCCACTTATCAAGAACATTGCATGGTCCTTTTAATAATTGTAATCTATCATCCCATGCATCGATGTTGGAATCCTTAAAAaCTTATCTATACACTCATGTCTGACGTTTCCAATATGTTCACTGTTAACAATTTTGCAGGTCACCTCTTCCACCATATGTTAAGTTTCTCCATACGTTGGGCTATCCGACTATATCAGATCCTTACACACTCAATTTATCAATCCAGACTCCTGGTAGCCGCTTATCAACGTTGCTGCCTCACTCTTGCTATCTCTCTTTCGCCCCTCCAGAGGTATTTGACAATTCTGCCACCTTTTTATGCTATTTCTCTTCTGCAATACAAAGTTTAAACTAAACTAAGGGCAATGCGTAAGGGGAAAAAGAACAAAATCTGAGTGAGCAGAGTCATGCTATAAAAATCTAGTTAGTAATGTATTATAATTTCATTATTACGAGCATGATACATGCAGATTTAAGGGAGATTGTATATAACCACGTACTAAATTGAACAATCTTATCAAATTAAAATTGAACTGATATAGTGCTTGAGAGGTGAATTGAATTAATTGATATCCCTAAGTTCTTTTTATGGAATATTAGTTCAACATACAACTTTAGGTGCGAACCCAATTTACTATAGTGAATTGTATCATTTTGTTATGAATTACTTCAACTTAATTTCAAGTGAAATGTTTACTTTCTGGACTTTTGTCAGATTTTTATGTCAATTTGGCATCCTATCCTATGAACCCGTACTTCCTTGCTGACAGCGTTTACCATTTGTTGCCGTGTTTACTATTTCTTACTTATCCTCTTTTTCCTTCTCTATTATCGTGTTTACTATTTCCTATCCAAACTAAAATAGTTTGCATCCCTATCATAACTCAAAGACTGACAATCATTGGCTATAATATTAAATTGTCACCCCAAACACCCATAGTTTATATATAGTGCCTAATTTTATCCCCCAATTAAAATTCATTTGCAGTCTACAGCTATTATTTGGCAGGTACCAATCTGATGAACAGGCATGGTCAGACACAATTAAATCTGTGAGCAGTGGAGCAACTGTTAGGATTAGTACCATGGACTGGCTGGGTCAATCTGAATTTACCACACCCACAATTTACTGGTCGTCCTTGTCTTCTGCAATATCGGAGGTAAATTTCTACGTGACTTTTCCTGTCTGCTTCTGTGGTTAATTTTTCTTTTTTtAAACGTTCAAGGAACTATTAACCATAAATTTAAAAGTCAAGGAGTAGACTTAGGTTGTTGATCTTACAAAAATATTTAACAGACGGAAAGATACACAATAGTTAAGCACTCTTGAGATAACTGATGTTAAGTACATCCAAATCATGTGAGGCCGCCTGGATCTTCTACATCCTAACTACAACCCTTTCAAGTTTCAATACCGAGGTCATCTTGTTTGTACAATCTGGTAGAAAATGGATTACATTCTAGTTTTTAAATATTAACAAAACCATTTTATGTATAGGTCGTCTTTTCAAAAAATGCAACTGTCAGGTTATTGGTATCATACTGGACACATTTTGTCGAGGGCACTGATCCATACCTCAAGTCTCTCCTCTACTCAAATATCCTCTGTAACTCTTCAAAGTTCAACGATTGTTTTGGGAAAGTTGAGATCAAATACTATGTGGTTCCCGGATACAACGAAACAGGACCAGCACTTTATCACAACGGAACAAGAACGAAAAACGAATACCCAGGTTTTAGTAGAGTTCAACATGGAAAGTTTGCAGTTAGTGACGTTAGAGCACATATAGGAACAAGCAATCTTGGATGGGATTACTTTTATGTAACTTCTGGTGTCAGCTTTGGAACATACAACCCAGCTATTGTTAAGCAGCTTCAAGAAGTGTTTGATGCTGACTGGAATTCTCCTTATACTCTTCCTGTTAAAGCCATACAGGATGGCCCTACATTTTCCAGCTGATTTGCACTTGAATCAATAGAAGTTCGATGAAATTACAAAGGAACATGCTACAAATCATGTGGCTTATTATTTAGTACATCTCTACACTAGATTGGAAGTGTTACAGATTCAGTCTAGATGTGT

mRNA sequence

ATGAAGAAAGCTCAAACTCCAACTTTAACTACCATTTTCAGTGCTCTCTTCTTCTTCTTCTTCGTCAGCCAATTCCCGCCTGTTCTCTCCTTCACTCAATCACAATGCAAGGGTTGGCTTGTACAATCAATTCCCACAGATATGCCGCAGCTTCAGCTGGTTCAAGGCGTCCTCTCGACTCGAGATGTATTCGTATGGTTAGCTGGAAACTCGACGCGGCGTTTGGATATCATTGCTCAGTACTGGGAATTACTAGCTGGTCCTAATGATTCTCGATCAGGGGACTATGGATACTCGGATGAGGATTTGAAGAAATTTGGGGCTAATAAAGGTTATGATGTTTATTCAGCCATAGAGAAAGCTGCCGATCGCAATGTCAATGTCAGGCTGGTATCTCACTCGGGGGTATCTCCAAACTATGGAAAAGAACCAGCTGACTTAGCATCTGGAAGGCCAAACGTGCAGAATGTAACTTTATTACTTGGTGATTGGTATGGATCAGGCATAGTCCACTCCAAAGTTTGGATATCTGATGATAGAGATGTGTATATAGGGTCTGCAAACCAGGACTGGAAATCTCTTTCACAGGTGAAGGAACTTGGAATATATCTTACAGGCTGTCCAAAAATAGCAGCTCATGTCAAGGTCTATTTTGAAAATTTATTGAAACTTGCATTTCTTAATCGCACGGATTACACAAGAACAATATTTGACCACCAGTGGCAAACACAAAGAAAAGTTCCTTGCTGGTCATATTTTATTGATCCCGAGTCTAGATGCAGGTCACCTCTTCCACCATATGTTAAGTTTCTCCATACGTTGGGCTATCCGACTATATCAGATCCTTACACACTCAATTTATCAATCCAGACTCCTGGTAGCCGCTTATCAACGTTGCTGCCTCACTCTTGCTATCTCTCTTTCGCCCCTCCAGAGCTATTATTTGGCAGGTACCAATCTGATGAACAGGCATGGTCAGACACAATTAAATCTGTGAGCAGTGGAGCAACTGTTAGGATTAGTACCATGGACTGGCTGGGTCAATCTGAATTTACCACACCCACAATTTACTGGTCGTCCTTGTCTTCTGCAATATCGGAGGTCGTCTTTTCAAAAAATGCAACTGTCAGGTTATTGGTATCATACTGGACACATTTTGTCGAGGGCACTGATCCATACCTCAAGTCTCTCCTCTACTCAAATATCCTCTGTAACTCTTCAAAGTTCAACGATTGTTTTGGGAAAGTTGAGATCAAATACTATGTGGTTCCCGGATACAACGAAACAGGACCAGCACTTTATCACAACGGAACAAGAACGAAAAACGAATACCCAGGTTTTAGTAGAGTTCAACATGGAAAGTTTGCAGTTAGTGACGTTAGAGCACATATAGGAACAAGCAATCTTGGATGGGATTACTTTTATGTAACTTCTGGTGTCAGCTTTGGAACATACAACCCAGCTATTGTTAAGCAGCTTCAAGAAGTGTTTGATGCTGACTGGAATTCTCCTTATACTCTTCCTGTTAAAGCCATACAGGATGGCCCTACATTTTCCAGCTGATTTGCACTTGAATCAATAGAAGTTCGATGAAATTACAAAGGAACATGCTACAAATCATGTGGCTTATTATTTAGTACATCTCTACACTAGATTGGAAGTGTTACAGATTCAGTCTAGATGTGT

Coding sequence (CDS)

ATGAAGAAAGCTCAAACTCCAACTTTAACTACCATTTTCAGTGCTCTCTTCTTCTTCTTCTTCGTCAGCCAATTCCCGCCTGTTCTCTCCTTCACTCAATCACAATGCAAGGGTTGGCTTGTACAATCAATTCCCACAGATATGCCGCAGCTTCAGCTGGTTCAAGGCGTCCTCTCGACTCGAGATGTATTCGTATGGTTAGCTGGAAACTCGACGCGGCGTTTGGATATCATTGCTCAGTACTGGGAATTACTAGCTGGTCCTAATGATTCTCGATCAGGGGACTATGGATACTCGGATGAGGATTTGAAGAAATTTGGGGCTAATAAAGGTTATGATGTTTATTCAGCCATAGAGAAAGCTGCCGATCGCAATGTCAATGTCAGGCTGGTATCTCACTCGGGGGTATCTCCAAACTATGGAAAAGAACCAGCTGACTTAGCATCTGGAAGGCCAAACGTGCAGAATGTAACTTTATTACTTGGTGATTGGTATGGATCAGGCATAGTCCACTCCAAAGTTTGGATATCTGATGATAGAGATGTGTATATAGGGTCTGCAAACCAGGACTGGAAATCTCTTTCACAGGTGAAGGAACTTGGAATATATCTTACAGGCTGTCCAAAAATAGCAGCTCATGTCAAGGTCTATTTTGAAAATTTATTGAAACTTGCATTTCTTAATCGCACGGATTACACAAGAACAATATTTGACCACCAGTGGCAAACACAAAGAAAAGTTCCTTGCTGGTCATATTTTATTGATCCCGAGTCTAGATGCAGGTCACCTCTTCCACCATATGTTAAGTTTCTCCATACGTTGGGCTATCCGACTATATCAGATCCTTACACACTCAATTTATCAATCCAGACTCCTGGTAGCCGCTTATCAACGTTGCTGCCTCACTCTTGCTATCTCTCTTTCGCCCCTCCAGAGCTATTATTTGGCAGGTACCAATCTGATGAACAGGCATGGTCAGACACAATTAAATCTGTGAGCAGTGGAGCAACTGTTAGGATTAGTACCATGGACTGGCTGGGTCAATCTGAATTTACCACACCCACAATTTACTGGTCGTCCTTGTCTTCTGCAATATCGGAGGTCGTCTTTTCAAAAAATGCAACTGTCAGGTTATTGGTATCATACTGGACACATTTTGTCGAGGGCACTGATCCATACCTCAAGTCTCTCCTCTACTCAAATATCCTCTGTAACTCTTCAAAGTTCAACGATTGTTTTGGGAAAGTTGAGATCAAATACTATGTGGTTCCCGGATACAACGAAACAGGACCAGCACTTTATCACAACGGAACAAGAACGAAAAACGAATACCCAGGTTTTAGTAGAGTTCAACATGGAAAGTTTGCAGTTAGTGACGTTAGAGCACATATAGGAACAAGCAATCTTGGATGGGATTACTTTTATGTAACTTCTGGTGTCAGCTTTGGAACATACAACCCAGCTATTGTTAAGCAGCTTCAAGAAGTGTTTGATGCTGACTGGAATTCTCCTTATACTCTTCCTGTTAAAGCCATACAGGATGGCCCTACATTTTCCAGCTGA

Protein sequence

MKKAQTPTLTTIFSALFFFFFVSQFPPVLSFTQSQCKGWLVQSIPTDMPQLQLVQGVLSTRDVFVWLAGNSTRRLDIIAQYWELLAGPNDSRSGDYGYSDEDLKKFGANKGYDVYSAIEKAADRNVNVRLVSHSGVSPNYGKEPADLASGRPNVQNVTLLLGDWYGSGIVHSKVWISDDRDVYIGSANQDWKSLSQVKELGIYLTGCPKIAAHVKVYFENLLKLAFLNRTDYTRTIFDHQWQTQRKVPCWSYFIDPESRCRSPLPPYVKFLHTLGYPTISDPYTLNLSIQTPGSRLSTLLPHSCYLSFAPPELLFGRYQSDEQAWSDTIKSVSSGATVRISTMDWLGQSEFTTPTIYWSSLSSAISEVVFSKNATVRLLVSYWTHFVEGTDPYLKSLLYSNILCNSSKFNDCFGKVEIKYYVVPGYNETGPALYHNGTRTKNEYPGFSRVQHGKFAVSDVRAHIGTSNLGWDYFYVTSGVSFGTYNPAIVKQLQEVFDADWNSPYTLPVKAIQDGPTFSS*
BLAST of Cucsa.271500 vs. Swiss-Prot
Match: PLDY_DICDI (Phospholipase D Y OS=Dictyostelium discoideum GN=pldY PE=2 SV=1)

HSP 1 Score: 188.7 bits (478), Expect = 1.6e-46
Identity = 140/500 (28.00%), Postives = 241/500 (48.20%), Query Frame = 1

Query: 16  LFFFFFVSQFPPVLS-FTQSQCKGWLVQSIPTDMPQLQLVQGVLSTRDVFVWLAGNSTRR 75
           +   FFV+    ++S  T + C+G  +Q I   +P    +   LST D ++ L  N+   
Sbjct: 9   IIVLFFVNVNSKIISESTLNSCQGGTIQ-IAESIPLGLDISTNLSTHDAWMDLITNAQES 68

Query: 76  LDIIAQYWELLAGPNDSRSGDYGYSDEDLKKFGANKGYDVYSAIEKAADRNVNVRLVSHS 135
           +D+   Y+ LL G           SD D   +G   G D+++AI +A  R +NVR+V + 
Sbjct: 69  IDLGFFYFTLLGG-----------SDLD-PVYGGQLGIDIFNAIVEAHSRGINVRIVQNE 128

Query: 136 GVSPNYGKEPADLASGRPNVQNVTLLLGDWY---GSGIVHSKVWISDDRDVYIGSANQDW 195
                   E   LA     V+++     DW    GSG++H+K+ I D+   Y+GSAN DW
Sbjct: 129 PSESFPDTETQTLAKLGIQVRSI-----DWVSLVGSGVLHTKLIIIDESSAYVGSANADW 188

Query: 196 KSLSQVKELGIYLTGCPKIAAHVKVYFENLLKLAFLNRTDYTRTIFDHQWQTQRKVPCWS 255
            SL+QVKELGI L  CP + A  ++ F+      + N  D+T ++  + W +  +    +
Sbjct: 189 SSLAQVKELGIVLKNCPTMVADTEIAFQQ-----YWNAADFT-SLPINDWGSNYQ----A 248

Query: 256 YFIDPESRCRSPLPPYVKFLHTLGYPTISDPYTLNLSIQTPGSRLSTLLPHSCYLSFAPP 315
            F +               + +L     ++    N +    GS  + +  +  +L+ +PP
Sbjct: 249 LFNNTN-------------MASLSLNNNNNNNNKNKNNDNDGSGTNQM--YEMFLAVSPP 308

Query: 316 ELLFGRYQSDEQAWSDTIKSVSSGATVRISTMDWLGQSEFTTPTIYWSSLSSAISEVVFS 375
           +        D  A  D I +     ++ I+ MD+   + +  P  YW  + +A+    F+
Sbjct: 309 QFQSTYRTGDIDALMDAINNADQ--SICITVMDYTPTTLYNDPNTYWPLIDNALRAAAFN 368

Query: 376 KNATVRLLVSYWTHFVEGTDPYLKSLLYS-NILCNSSKFNDCFGKVEIKYYVVPGYNETG 435
           +N  VR+L+S+W +    T P +   L+S N + N          ++++++VVP ++ T 
Sbjct: 369 RNVQVRMLISHWNY----TSPIIPQWLHSLNQVDN----------IQVRWFVVPDFS-TN 428

Query: 436 PALYHNGTRTKNEYPGFSRVQHGKFAVSDVRAHIGTSNLGWDYFYVTSGVSFGTYNPAIV 495
           P +             ++RV H KF V++ ++++GTSN   DYF VT G+S+  +N    
Sbjct: 429 PQI------------PYTRVNHAKFMVTEKQSYVGTSNWSQDYFTVTGGLSYNVFNNDFT 436

Query: 496 KQLQEVFDADWNSPYTLPVK 511
            QLQ +F+ DWNSPYT P++
Sbjct: 489 NQLQSIFNRDWNSPYTQPIQ 436

BLAST of Cucsa.271500 vs. Swiss-Prot
Match: PLD3_PONAB (Phospholipase D3 OS=Pongo abelii GN=PLD3 PE=2 SV=1)

HSP 1 Score: 141.4 bits (355), Expect = 2.9e-32
Identity = 133/525 (25.33%), Postives = 221/525 (42.10%), Query Frame = 1

Query: 2   KKAQTPTLTTI-----FSALFFFFFVSQFPPVLSFTQSQ--------CKGWLVQSIPTDM 61
           KKA+   L  I     F AL    F+ ++  +  F  +Q        C+  LV+SIP  +
Sbjct: 34  KKARWVLLVLILAVVGFGALMTQLFLWEYGDLHLFGPNQRPAPCYDPCEAVLVESIPEGL 93

Query: 62  PQLQLVQGVLSTRDVFVWLAGNSTRRLDIIAQYWELLAGPNDSRSGDYGYSDEDLKKFGA 121
                  G  ST   ++ L   +   LDI + YW L              +D   ++  A
Sbjct: 94  DFPNASTGNPSTSQAWLGLLAGAHSSLDIASFYWTLTN------------NDTHTQEPSA 153

Query: 122 NKGYDVYSAIEKAADRNVNVRLVSHSGVSPNYGKEPADLASGRPNVQNVTLLLGDWYGSG 181
            +G +V   ++  A + VNVR+       PN  +  ADL +   +   V ++       G
Sbjct: 154 QQGEEVLRQLQTLAPKGVNVRIAVSK---PNGPQPQADLQALLQSGAQVRMVDMQKLTHG 213

Query: 182 IVHSKVWISDDRDVYIGSANQDWKSLSQVKELGIYLTGCPKIAAHVKVYFENLLKLAFLN 241
           ++H+K W+ D    Y+GSAN DW+SL+QVKELG+ +  C  +A                 
Sbjct: 214 VLHTKFWVVDQTHFYLGSANMDWRSLTQVKELGVVMYNCSCLA----------------- 273

Query: 242 RTDYTRTIFDHQW---QTQRKVP-CWSYFIDPESRCRSPLPPYVKFLHTLGYPTISDPYT 301
             D T+ IF+  W   Q    +P  W  F D      +P+   +      G P ++    
Sbjct: 274 -RDLTK-IFEAYWFLGQAGSSIPSTWPRFYDTRYNQETPMEICLN-----GTPALA---- 333

Query: 302 LNLSIQTPGSRLSTLLPHSCYLSFAPPELLFGRYQSDEQAWSDTIKSVSSGATVRISTMD 361
                               YL+ APP L       D +A  + + +  S   + ++ M+
Sbjct: 334 --------------------YLASAPPPLCPSGRTPDLKALLNVVDNARS--FIYVAVMN 393

Query: 362 WLGQSEFTTPTIYWSSLSSAISEVVFSKNATVRLLVSYWTHFVEGTDPYLKSLLYSNILC 421
           +L   EF+ P  +W ++   +    + +   VRLL+S W H    ++P +++ L S    
Sbjct: 394 YLPTLEFSHPHRFWPAIDDGLRRAAYERGVKVRLLISCWGH----SEPSMRAFLLS---L 453

Query: 422 NSSKFNDCFGKVEIKYYVVPGYNETGPALYHNGTRTKNEYPGFSRVQHGKFAVSDVRAHI 481
            + + N     +++K +VVP                +   P ++RV H K+ V++   +I
Sbjct: 454 AALRDNHTHSDIQVKLFVVP------------ADEAQARIP-YARVNHNKYMVTERATYI 473

Query: 482 GTSNLGWDYFYVTSGVS-FGTYN--PAIVKQLQEVFDADWNSPYT 507
           GTSN   +YF  T+G S   T N    +  QL+ +F  DW+SPY+
Sbjct: 514 GTSNWSGNYFTETAGTSLLVTQNGRGGLRSQLEAIFLRDWDSPYS 473

BLAST of Cucsa.271500 vs. Swiss-Prot
Match: PLD3_HUMAN (Phospholipase D3 OS=Homo sapiens GN=PLD3 PE=1 SV=1)

HSP 1 Score: 141.4 bits (355), Expect = 2.9e-32
Identity = 135/526 (25.67%), Postives = 223/526 (42.40%), Query Frame = 1

Query: 2   KKAQTPTLTTI-----FSALFFFFFVSQFPPVLSFTQSQ--------CKGWLVQSIPTDM 61
           KKA+   L  I     F AL    F+ ++  +  F  +Q        C+  LV+SIP  +
Sbjct: 34  KKARWVLLVLILAVVGFGALMTQLFLWEYGDLHLFGPNQRPAPCYDPCEAVLVESIPEGL 93

Query: 62  PQLQLVQGVLSTRDVFVWLAGNSTRRLDIIAQYWELLAGPNDSRSGDYGYSDEDLKKFGA 121
                  G  ST   ++ L   +   LDI + YW L              +D   ++  A
Sbjct: 94  DFPNASTGNPSTSQAWLGLLAGAHSSLDIASFYWTLTN------------NDTHTQEPSA 153

Query: 122 NKGYDVYSAIEKAADRNVNVRLVSHSGVSPNYGKEP-ADLASGRPNVQNVTLLLGDWYGS 181
            +G +V   ++  A + VNVR+     VS   G +P ADL +   +   V ++       
Sbjct: 154 QQGEEVLRQLQTLAPKGVNVRIA----VSKPSGPQPQADLQALLQSGAQVRMVDMQKLTH 213

Query: 182 GIVHSKVWISDDRDVYIGSANQDWKSLSQVKELGIYLTGCPKIAAHVKVYFENLLKLAFL 241
           G++H+K W+ D    Y+GSAN DW+SL+QVKELG+ +  C  +A                
Sbjct: 214 GVLHTKFWVVDQTHFYLGSANMDWRSLTQVKELGVVMYNCSCLA---------------- 273

Query: 242 NRTDYTRTIFDHQW---QTQRKVP-CWSYFIDPESRCRSPLPPYVKFLHTLGYPTISDPY 301
              D T+ IF+  W   Q    +P  W  F D      +P+   +      G P ++   
Sbjct: 274 --RDLTK-IFEAYWFLGQAGSSIPSTWPRFYDTRYNQETPMEICLN-----GTPALA--- 333

Query: 302 TLNLSIQTPGSRLSTLLPHSCYLSFAPPELLFGRYQSDEQAWSDTIKSVSSGATVRISTM 361
                                YL+ APP L       D +A  + + +  S   + ++ M
Sbjct: 334 ---------------------YLASAPPPLCPSGRTPDLKALLNVVDNARS--FIYVAVM 393

Query: 362 DWLGQSEFTTPTIYWSSLSSAISEVVFSKNATVRLLVSYWTHFVEGTDPYLKSLLYSNIL 421
           ++L   EF+ P  +W ++   +    + +   VRLL+S W H    ++P +++ L S   
Sbjct: 394 NYLPTLEFSHPHRFWPAIDDGLRRATYERGVKVRLLISCWGH----SEPSMRAFLLS--- 453

Query: 422 CNSSKFNDCFGKVEIKYYVVPGYNETGPALYHNGTRTKNEYPGFSRVQHGKFAVSDVRAH 481
             + + N     +++K +VVP                +   P ++RV H K+ V++   +
Sbjct: 454 LAALRDNHTHSDIQVKLFVVP------------ADEAQARIP-YARVNHNKYMVTERATY 473

Query: 482 IGTSNLGWDYFYVTSGVS-FGTYN--PAIVKQLQEVFDADWNSPYT 507
           IGTSN   +YF  T+G S   T N    +  QL+ +F  DW+SPY+
Sbjct: 514 IGTSNWSGNYFTETAGTSLLVTQNGRGGLRSQLEAIFLRDWDSPYS 473

BLAST of Cucsa.271500 vs. Swiss-Prot
Match: PLD3_MACFA (Phospholipase D3 OS=Macaca fascicularis GN=PLD3 PE=2 SV=1)

HSP 1 Score: 141.4 bits (355), Expect = 2.9e-32
Identity = 134/533 (25.14%), Postives = 218/533 (40.90%), Query Frame = 1

Query: 2   KKAQTPTLTTI-----FSALFFFFFVSQFPPVLSFTQSQ--------CKGWLVQSIPTDM 61
           KKA+   L  I     F AL    F+ ++  +  F  +Q        C+  LV+SIP  +
Sbjct: 34  KKARWVLLVLILAVVGFGALMTQLFLWEYGDLHLFGPNQRPAPCYDPCEAVLVESIPEGL 93

Query: 62  PQLQLVQGVLSTRDVFVWLAGNSTRRLDIIAQYWELLAGPNDSRSGDYGYSDEDLKKFGA 121
                  G  ST   ++ L   +   LDI + YW L              +D   ++  A
Sbjct: 94  DFPNASTGNPSTSQAWLGLLAGAHSSLDIASFYWTLTN------------NDTHTQEPSA 153

Query: 122 NKGYDVYSAIEKAADRNVNVRLVSHSGVSPNYGKEPADLASGRPNVQNVTLLLGDWYGSG 181
            +G +V   ++  A + VNVR+       PN  +   DL +   +   V ++       G
Sbjct: 154 QQGEEVLRQLQTLAPKGVNVRIAVSK---PNGPQPQTDLQALLQSGAQVRMVDMQKLTHG 213

Query: 182 IVHSKVWISDDRDVYIGSANQDWKSLSQVKELGIYLTGCPKIAAHVKVYFENLLKLAFLN 241
           ++H+K W+ D    Y+GSAN DW+SL+QVKELG+ +  C  +A                 
Sbjct: 214 VLHTKFWVVDQTHFYLGSANMDWRSLTQVKELGVVMYNCSCLA----------------- 273

Query: 242 RTDYTRTIFDHQW---QTQRKVP-CWSYFIDPESRCRSPLPPYVKFLHTLGYPTISDPYT 301
             D T+ IF+  W   Q    +P  W  F D      +P+   +      G P ++    
Sbjct: 274 -RDLTK-IFEAYWFLGQAGSSIPSTWPRFYDTRYNQETPMEICLN-----GTPALA---- 333

Query: 302 LNLSIQTPGSRLSTLLPHSCYLSFAPPELLFGRYQSDEQAWSDTIKSVSSGATVRISTMD 361
                               YL+ APP L       D +A  + + +  S   + I+ M+
Sbjct: 334 --------------------YLASAPPPLCPSGRTPDLKALLNVVDNARS--FIYIAVMN 393

Query: 362 WLGQSEFTTPTIYWSSLSSAISEVVFSKNATVRLLVSYWTHFVEGTDPYLKSLLYSNILC 421
           +L   EF+ P  +W ++   +    + +   VRLLVS W H       +L SL       
Sbjct: 394 YLPTLEFSHPHRFWPAIDDGLRRAAYERGVKVRLLVSCWRHSESSMRAFLLSLA------ 453

Query: 422 NSSKFNDCFGKVEIKYYVVPGYNETGPALYHNGTRTKNEYPGFSRVQHGKFAVSDVRAHI 481
            + + N     +++K +VVP                +   P ++RV H K+ V++   +I
Sbjct: 454 -ALRDNHTHSDIQVKLFVVP------------ADEAQARIP-YARVNHNKYMVTERATYI 481

Query: 482 GTSNLGWDYFYVTSGVS---FGTYNPAIVKQLQEVFDADWNSPYTLPVKAIQD 515
           GTSN   +YF  T+G S         ++  QL+ +F  DW+SPY+  + A  D
Sbjct: 514 GTSNWSGNYFTETAGTSLLVMQNGRGSLRSQLEAIFLRDWDSPYSHDLDASAD 481

BLAST of Cucsa.271500 vs. Swiss-Prot
Match: K4_VACCW (Phospholipase-D-like protein K4 OS=Vaccinia virus (strain Western Reserve) GN=VACWR035 PE=3 SV=1)

HSP 1 Score: 127.5 bits (319), Expect = 4.3e-28
Identity = 128/476 (26.89%), Postives = 200/476 (42.02%), Query Frame = 1

Query: 40  LVQSIPTDMPQLQLVQGVLSTRDVFVWLAGNSTRRLDIIAQYWELLAGPNDSRSGDYGYS 99
           + ++IP  M   Q  +  LST +++  +  N+T+ LDI + YW L              S
Sbjct: 10  ITETIPIGM---QFDKVYLSTFNMWREILSNTTKTLDISSFYWSL--------------S 69

Query: 100 DEDLKKFGANKGYDVYSAIEKAADRNVNVRLVSHSGVSPNYGKEPADLASGRPNVQNVTL 159
           DE     G N G  + + I +   R V VR+  +    P    E   +A       ++T 
Sbjct: 70  DE----VGTNFGTIILNKIVQLPKRGVRVRVAVNKSNKPLKDVERLQMAGVEVRYIDITN 129

Query: 160 LLGDWYGSGIVHSKVWISDDRDVYIGSANQDWKSLSQVKELGIYLTGCPKIAAHVKVYFE 219
           +LG     G++H+K WISD+  +Y+GSAN DW+SL+QVKELGI                 
Sbjct: 130 ILG-----GVLHTKFWISDNTHIYLGSANMDWRSLTQVKELGI----------------- 189

Query: 220 NLLKLAFLNRT---DYTRTIFDHQWQTQ-RKVPC-WSYFIDPESRCRSPLPPYVKFLHTL 279
                 F NR    D T+ IF+  W      +P  W  F           P Y    H L
Sbjct: 190 ----AIFNNRNLAADLTQ-IFEVYWYLGVNNLPYNWKNFY----------PSYYNTDHPL 249

Query: 280 GYPTISDPYTLNLSIQTPGSRLSTLLPHSCYLSFAPPELLFGRYQSDEQAWSDTIKSVSS 339
                        SI   G      +PHS +++ AP +L      +D  A    I++ S 
Sbjct: 250 -------------SINVSG------VPHSVFIASAPQQLCTMERTNDLTALLSCIRNASK 309

Query: 340 GATVRISTMDWLGQSEFTTPTI-YWSSLSSAISEVVFSKNATVRLLVSYWTHFVEGTDPY 399
              V +S M+++         I +W  +   +      +  +V+LL+S W    + +   
Sbjct: 310 --FVYVSVMNFIPIIYSKAGNILFWPYIEDELRRAAIDRQVSVKLLISCW----QRSSFI 369

Query: 400 LKSLLYSNILCNSSKFNDCFGKVEIKYYVVPGYNETGPALYHNGTRTKNEYPGFSRVQHG 459
           +++ L S  +  S   N     +E+K ++VP  +   P               +SRV H 
Sbjct: 370 MRNFLRSIAMLKSKNIN-----IEVKLFIVPDADPPIP---------------YSRVNHA 382

Query: 460 KFAVSDVRAHIGTSNLGWDYFYVTSGVSFGTY---NPAIVKQLQEVFDADWNSPYT 507
           K+ V+D  A+IGTSN   +YF  T G S          + +QL+++F  DWNS Y+
Sbjct: 430 KYMVTDKTAYIGTSNWTGNYFTDTCGASINITPDDGLGLRQQLEDIFMRDWNSKYS 382

BLAST of Cucsa.271500 vs. TrEMBL
Match: A0A0A0L498_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G651930 PE=4 SV=1)

HSP 1 Score: 1076.6 bits (2783), Expect = 0.0e+00
Identity = 520/520 (100.00%), Postives = 520/520 (100.00%), Query Frame = 1

Query: 1   MKKAQTPTLTTIFSALFFFFFVSQFPPVLSFTQSQCKGWLVQSIPTDMPQLQLVQGVLST 60
           MKKAQTPTLTTIFSALFFFFFVSQFPPVLSFTQSQCKGWLVQSIPTDMPQLQLVQGVLST
Sbjct: 1   MKKAQTPTLTTIFSALFFFFFVSQFPPVLSFTQSQCKGWLVQSIPTDMPQLQLVQGVLST 60

Query: 61  RDVFVWLAGNSTRRLDIIAQYWELLAGPNDSRSGDYGYSDEDLKKFGANKGYDVYSAIEK 120
           RDVFVWLAGNSTRRLDIIAQYWELLAGPNDSRSGDYGYSDEDLKKFGANKGYDVYSAIEK
Sbjct: 61  RDVFVWLAGNSTRRLDIIAQYWELLAGPNDSRSGDYGYSDEDLKKFGANKGYDVYSAIEK 120

Query: 121 AADRNVNVRLVSHSGVSPNYGKEPADLASGRPNVQNVTLLLGDWYGSGIVHSKVWISDDR 180
           AADRNVNVRLVSHSGVSPNYGKEPADLASGRPNVQNVTLLLGDWYGSGIVHSKVWISDDR
Sbjct: 121 AADRNVNVRLVSHSGVSPNYGKEPADLASGRPNVQNVTLLLGDWYGSGIVHSKVWISDDR 180

Query: 181 DVYIGSANQDWKSLSQVKELGIYLTGCPKIAAHVKVYFENLLKLAFLNRTDYTRTIFDHQ 240
           DVYIGSANQDWKSLSQVKELGIYLTGCPKIAAHVKVYFENLLKLAFLNRTDYTRTIFDHQ
Sbjct: 181 DVYIGSANQDWKSLSQVKELGIYLTGCPKIAAHVKVYFENLLKLAFLNRTDYTRTIFDHQ 240

Query: 241 WQTQRKVPCWSYFIDPESRCRSPLPPYVKFLHTLGYPTISDPYTLNLSIQTPGSRLSTLL 300
           WQTQRKVPCWSYFIDPESRCRSPLPPYVKFLHTLGYPTISDPYTLNLSIQTPGSRLSTLL
Sbjct: 241 WQTQRKVPCWSYFIDPESRCRSPLPPYVKFLHTLGYPTISDPYTLNLSIQTPGSRLSTLL 300

Query: 301 PHSCYLSFAPPELLFGRYQSDEQAWSDTIKSVSSGATVRISTMDWLGQSEFTTPTIYWSS 360
           PHSCYLSFAPPELLFGRYQSDEQAWSDTIKSVSSGATVRISTMDWLGQSEFTTPTIYWSS
Sbjct: 301 PHSCYLSFAPPELLFGRYQSDEQAWSDTIKSVSSGATVRISTMDWLGQSEFTTPTIYWSS 360

Query: 361 LSSAISEVVFSKNATVRLLVSYWTHFVEGTDPYLKSLLYSNILCNSSKFNDCFGKVEIKY 420
           LSSAISEVVFSKNATVRLLVSYWTHFVEGTDPYLKSLLYSNILCNSSKFNDCFGKVEIKY
Sbjct: 361 LSSAISEVVFSKNATVRLLVSYWTHFVEGTDPYLKSLLYSNILCNSSKFNDCFGKVEIKY 420

Query: 421 YVVPGYNETGPALYHNGTRTKNEYPGFSRVQHGKFAVSDVRAHIGTSNLGWDYFYVTSGV 480
           YVVPGYNETGPALYHNGTRTKNEYPGFSRVQHGKFAVSDVRAHIGTSNLGWDYFYVTSGV
Sbjct: 421 YVVPGYNETGPALYHNGTRTKNEYPGFSRVQHGKFAVSDVRAHIGTSNLGWDYFYVTSGV 480

Query: 481 SFGTYNPAIVKQLQEVFDADWNSPYTLPVKAIQDGPTFSS 521
           SFGTYNPAIVKQLQEVFDADWNSPYTLPVKAIQDGPTFSS
Sbjct: 481 SFGTYNPAIVKQLQEVFDADWNSPYTLPVKAIQDGPTFSS 520

BLAST of Cucsa.271500 vs. TrEMBL
Match: A0A067KBJ9_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_07083 PE=4 SV=1)

HSP 1 Score: 729.6 bits (1882), Expect = 2.8e-207
Identity = 345/501 (68.86%), Postives = 409/501 (81.64%), Query Frame = 1

Query: 20  FFVSQFPPVLSFTQSQCKGWLVQSIPTDMPQLQLVQGVLSTRDVFVWLAGNSTRRLDIIA 79
           FF++Q         S+CK WLVQSIPTDMPQL+ V GVL+T DV  WLAGNST+ LDIIA
Sbjct: 20  FFITQ-------ADSKCKAWLVQSIPTDMPQLRRVPGVLTTGDVLRWLAGNSTKGLDIIA 79

Query: 80  QYWELLAGPNDSRSGDYGYSDEDLKKFGANKGYDVYSAIEKAADRNVNVRLVSHSGVSPN 139
           QYW+L+A   D RSGDYGYS  D++KFGA +G+ VY AIE AADRNV++RL+ HSGV P+
Sbjct: 80  QYWQLIANSKDPRSGDYGYSKSDMEKFGAFEGFGVYKAIENAADRNVSIRLLQHSGVYPD 139

Query: 140 YGKEPADLASGRPNVQNVTLLLGDWYGSGIVHSKVWISDDRDVYIGSANQDWKSLSQVKE 199
           + KEP DLASGR NV NVTLLLG+W+GSGIVH+KVWISD RDVYIGSAN DWKSL+QVKE
Sbjct: 140 FTKEPNDLASGRLNVDNVTLLLGEWWGSGIVHAKVWISDSRDVYIGSANNDWKSLTQVKE 199

Query: 200 LGIYLTGCPKIAAHVKVYFENLLKLAFLNRTDYTRTIFDHQWQTQRKVPCWSYFIDPESR 259
           +GIYL GC KI   V+ YFENL KLA LN + YTR+++D QWQ  R VPCWS+FID ++R
Sbjct: 200 VGIYLVGCKKIVGKVETYFENLWKLAHLNSSAYTRSVWDQQWQINRTVPCWSHFIDSKAR 259

Query: 260 CRSPLPPYVKFLHTLGYPTISDPYTLNLSIQTPGSRLSTLLPHSCYLSFAPPELLFGRYQ 319
           CRSPLP +V+  H  GYP +SDP+ + + I+TPG R STL P+  YLSFAPPEL FG+YQ
Sbjct: 260 CRSPLPTFVEVPHVAGYPVLSDPHMIKVPIKTPGQRFSTLEPYLSYLSFAPPELSFGKYQ 319

Query: 320 SDEQAWSDTIKSVSSGATVRISTMDWLGQSEFTTPTIYWSSLSSAISEVVFSKNATVRLL 379
           +DEQ W DTIKSV SG+TVRI+TMDWLGQS+FT   +YWSSLSSAISEV+FSK+ATV++L
Sbjct: 320 TDEQGWIDTIKSVGSGSTVRINTMDWLGQSQFTKEKVYWSSLSSAISEVIFSKHATVKVL 379

Query: 380 VSYWTHFVEGTDPYLKSLLYSNILCNSSKFNDCFGKVEIKYYVVPGYNETGPALYHNGTR 439
           V+YW HF++ TD YLKSLLYS+ILCNSSK+N C GKVEIKYYVVPGYN TGPA   NG R
Sbjct: 380 VAYWAHFIDNTDQYLKSLLYSDILCNSSKYNKCHGKVEIKYYVVPGYNLTGPAT-RNGKR 439

Query: 440 TKNEYPGFSRVQHGKFAVSDVRAHIGTSNLGWDYFYVTSGVSFGTYNPAIVKQLQEVFDA 499
           T N+YP F+RV HGK+AVSDVRAHIGTSNL WDYFY T+GVSFGTYNPAIV QLQ++FDA
Sbjct: 440 TGNKYPAFTRVNHGKYAVSDVRAHIGTSNLVWDYFYTTAGVSFGTYNPAIVLQLQKIFDA 499

Query: 500 DWNSPYTLPVKAIQDGPTFSS 521
           DW+SPY +P + ++ G  FSS
Sbjct: 500 DWDSPYAVPFEGLEAGHAFSS 512

BLAST of Cucsa.271500 vs. TrEMBL
Match: V4UMB2_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10008002mg PE=4 SV=1)

HSP 1 Score: 729.6 bits (1882), Expect = 2.8e-207
Identity = 343/517 (66.34%), Postives = 412/517 (79.69%), Query Frame = 1

Query: 7   PTLTTIFSALFFFFFVSQFPPVL---SFTQSQCKGWLVQSIPTDMPQLQLVQGVLSTRDV 66
           P   T  S + F  F    P +    S ++++CK WLVQSIPTDMP L  V G++S+ +V
Sbjct: 3   PRYRTSLSLVIFILFTLYLPDITTSSSSSRTKCKAWLVQSIPTDMPHLPRVPGIVSSGNV 62

Query: 67  FVWLAGNSTRRLDIIAQYWELLAGPNDSRSGDYGYSDEDLKKFGANKGYDVYSAIEKAAD 126
             WLAGNST+RLDIIAQYW+L+A PN+SRSGDYGYS++D+K+FGA +G+DVY +IE AAD
Sbjct: 63  LKWLAGNSTKRLDIIAQYWQLIARPNNSRSGDYGYSEDDMKRFGAQEGFDVYKSIENAAD 122

Query: 127 RNVNVRLVSHSGVSPNYGKEPADLASGRPNVQNVTLLLGDWYGSGIVHSKVWISDDRDVY 186
           RNV VRL+ H GV P++  E ++LASGR NV+NVTLLLGDW+GSGIVH+KVWISD RDVY
Sbjct: 123 RNVRVRLLQHLGVYPDFTTEASNLASGRKNVENVTLLLGDWWGSGIVHAKVWISDCRDVY 182

Query: 187 IGSANQDWKSLSQVKELGIYLTGCPKIAAHVKVYFENLLKLAFLNRTDYTRTIFDHQWQT 246
           IGSAN DWKSL+QVKE+GIYL GCP IA  V+VYFENL  L+ LN + YTRT+ D QWQ 
Sbjct: 183 IGSANNDWKSLTQVKEVGIYLVGCPTIAKKVEVYFENLWTLSSLNSSTYTRTVSDQQWQA 242

Query: 247 QRKVPCWSYFIDPESRCRSPLPPYVKFLHTLGYPTISDPYTLNLSIQTPGSRLSTLLPHS 306
            R VPCWS+F+DP+ RCRSPLPP ++  H  GYP +SDPY    +++TPGS  STL PH 
Sbjct: 243 NRTVPCWSHFLDPKGRCRSPLPPVLEVPHVEGYPILSDPYMFRETMETPGSNYSTLQPHY 302

Query: 307 CYLSFAPPELLFGRYQSDEQAWSDTIKSVSSGATVRISTMDWLGQSEFTTPTIYWSSLSS 366
            YLSFAPPEL FG+Y +DEQAW DTIKSV + AT+RISTMDWLGQS++   T+YWS+LSS
Sbjct: 303 SYLSFAPPELSFGKYMADEQAWVDTIKSVGTRATLRISTMDWLGQSQYLKQTVYWSALSS 362

Query: 367 AISEVVFSKNATVRLLVSYWTHFVEGTDPYLKSLLYSNILCNSSKFNDCFGKVEIKYYVV 426
           A+SEVVFSK+A V++LV+YW HF+  TD YLKSLLYSN+LCNSSK+N C GKVEIKYY V
Sbjct: 363 AVSEVVFSKHANVKILVAYWAHFINNTDEYLKSLLYSNVLCNSSKYNKCSGKVEIKYYKV 422

Query: 427 PGYNETGPALYHNGTRTKNEYPGFSRVQHGKFAVSDVRAHIGTSNLGWDYFYVTSGVSFG 486
           PGYN TGPA+  NG+ T N YPGF+RV HGK+AVSD RAHIGTSNL WDYFY T+GVSFG
Sbjct: 423 PGYNLTGPAI-QNGSNTGNIYPGFTRVNHGKYAVSDSRAHIGTSNLVWDYFYTTAGVSFG 482

Query: 487 TYNPAIVKQLQEVFDADWNSPYTLPVKAIQDGPTFSS 521
           TYNPAIV QLQE+FDADWNSPY  PV+ + DG   +S
Sbjct: 483 TYNPAIVSQLQEIFDADWNSPYAFPVEELGDGHAHAS 518

BLAST of Cucsa.271500 vs. TrEMBL
Match: A0A067FW62_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g009992mg PE=4 SV=1)

HSP 1 Score: 729.6 bits (1882), Expect = 2.8e-207
Identity = 343/517 (66.34%), Postives = 412/517 (79.69%), Query Frame = 1

Query: 7   PTLTTIFSALFFFFFVSQFPPVL---SFTQSQCKGWLVQSIPTDMPQLQLVQGVLSTRDV 66
           P   T  S + F  F    P +    S ++++CK WLVQSIPTDMP L  V G++S+ +V
Sbjct: 3   PRYRTSLSLVIFILFTLYLPDITTSSSSSRTKCKAWLVQSIPTDMPHLPRVPGIVSSGNV 62

Query: 67  FVWLAGNSTRRLDIIAQYWELLAGPNDSRSGDYGYSDEDLKKFGANKGYDVYSAIEKAAD 126
             WLAGNST+RLDIIAQYW+L+A PN+SRSGDYGYS++D+K+FGA +G+DVY +IE AAD
Sbjct: 63  LKWLAGNSTKRLDIIAQYWQLIARPNNSRSGDYGYSEDDMKRFGAQEGFDVYKSIENAAD 122

Query: 127 RNVNVRLVSHSGVSPNYGKEPADLASGRPNVQNVTLLLGDWYGSGIVHSKVWISDDRDVY 186
           RNV VRL+ H GV P++  E ++LASGR NV+NVTLLLGDW+GSGIVH+KVWISD RDVY
Sbjct: 123 RNVRVRLLQHLGVYPDFTTEASNLASGRKNVENVTLLLGDWWGSGIVHAKVWISDCRDVY 182

Query: 187 IGSANQDWKSLSQVKELGIYLTGCPKIAAHVKVYFENLLKLAFLNRTDYTRTIFDHQWQT 246
           IGSAN DWKSL+QVKE+GIYL GCP IA  V+VYFENL  L+ LN + YTRT+ D QWQ 
Sbjct: 183 IGSANNDWKSLTQVKEVGIYLVGCPTIAKKVEVYFENLWTLSSLNSSTYTRTVSDQQWQA 242

Query: 247 QRKVPCWSYFIDPESRCRSPLPPYVKFLHTLGYPTISDPYTLNLSIQTPGSRLSTLLPHS 306
            R VPCWS+F+DP+ RCRSPLPP ++  H  GYP +SDPY    +++TPGS  STL PH 
Sbjct: 243 NRTVPCWSHFLDPKGRCRSPLPPVLEVPHVEGYPILSDPYMFRETMETPGSNYSTLQPHY 302

Query: 307 CYLSFAPPELLFGRYQSDEQAWSDTIKSVSSGATVRISTMDWLGQSEFTTPTIYWSSLSS 366
            YLSFAPPEL FG+Y +DEQAW DTIKSV + AT+RISTMDWLGQS++   T+YWS+LSS
Sbjct: 303 SYLSFAPPELSFGKYMADEQAWVDTIKSVGTRATLRISTMDWLGQSQYLKQTVYWSALSS 362

Query: 367 AISEVVFSKNATVRLLVSYWTHFVEGTDPYLKSLLYSNILCNSSKFNDCFGKVEIKYYVV 426
           A+SEVVFSK+A V++LV+YW HF+  TD YLKSLLYSN+LCNSSK+N C GKVEIKYY V
Sbjct: 363 AVSEVVFSKHANVKILVAYWAHFINNTDEYLKSLLYSNVLCNSSKYNKCSGKVEIKYYKV 422

Query: 427 PGYNETGPALYHNGTRTKNEYPGFSRVQHGKFAVSDVRAHIGTSNLGWDYFYVTSGVSFG 486
           PGYN TGPA+  NG+ T N YPGF+RV HGK+AVSD RAHIGTSNL WDYFY T+GVSFG
Sbjct: 423 PGYNLTGPAI-QNGSNTGNIYPGFTRVNHGKYAVSDSRAHIGTSNLVWDYFYTTAGVSFG 482

Query: 487 TYNPAIVKQLQEVFDADWNSPYTLPVKAIQDGPTFSS 521
           TYNPAIV QLQE+FDADWNSPY  PV+ + DG   +S
Sbjct: 483 TYNPAIVSQLQEIFDADWNSPYAFPVEELGDGHAHAS 518

BLAST of Cucsa.271500 vs. TrEMBL
Match: D7SV42_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_04s0008g06620 PE=4 SV=1)

HSP 1 Score: 724.2 bits (1868), Expect = 1.2e-205
Identity = 343/510 (67.25%), Postives = 407/510 (79.80%), Query Frame = 1

Query: 11  TIFSALFFFFFVSQFPPVLSFTQSQCKGWLVQSIPTDMPQLQLVQGVLSTRDVFVWLAGN 70
           T   ++F    V Q   V + + SQCK W+VQSIPT MP L  V GVLST DVF WLAGN
Sbjct: 5   TTLKSVFIVLLVIQALGVNAKSSSQCKAWIVQSIPTHMPHLPPVSGVLSTADVFQWLAGN 64

Query: 71  STRRLDIIAQYWELLAGPNDSRSGDYGYSDEDLKKFGANKGYDVYSAIEKAADRNVNVRL 130
           S+  LDIIAQYW+L A P+D  SGDYGYS  D+ KFGA++G+DVY A+E AADRN+++RL
Sbjct: 65  SSLSLDIIAQYWQLNAHPDDPLSGDYGYSKADMHKFGAHEGFDVYRALENAADRNISIRL 124

Query: 131 VSHSGVSPNYGKEPADLASGRPNVQNVTLLLGDWYGSGIVHSKVWISDDRDVYIGSANQD 190
           + HSGV P+Y KEP+DLASGRPNV+NVTLLLG+W+GSGIVH+KVWISD RDVYIGSAN D
Sbjct: 125 LQHSGVYPDYTKEPSDLASGRPNVENVTLLLGEWWGSGIVHAKVWISDSRDVYIGSANND 184

Query: 191 WKSLSQVKELGIYLTGCPKIAAHVKVYFENLLKLAFLNRTDYTRTIFDHQWQTQRKVPCW 250
           WKSL+QVKE+GIYL  CP IA  V++Y+ NL KLA LN + YT T++D QWQ  R+VPCW
Sbjct: 185 WKSLTQVKEVGIYLVDCPSIARQVEIYYNNLWKLASLNVSAYTTTVWDQQWQINRQVPCW 244

Query: 251 SYFIDPESRCRSPLPPYVKFLHTLGYPTISDPYTLNLSIQTPGSRLSTLLPHSCYLSFAP 310
           S+F+ P  RCRSPLP YV   H +GYPT+SDP      IQTPG   ST  P S  LSFAP
Sbjct: 245 SHFLTPRERCRSPLPRYVAVPHVVGYPTLSDPRMFKTPIQTPGHNCSTSKPQSSCLSFAP 304

Query: 311 PELLFGRYQSDEQAWSDTIKSVSSGATVRISTMDWLGQSEFTTPTIYWSSLSSAISEVVF 370
           PEL FG++Q+DEQAW DTIKSV +GATVRI++MDWLGQS++   T+YWSSLSSA+SEVVF
Sbjct: 305 PELSFGKHQADEQAWVDTIKSVGTGATVRINSMDWLGQSQYMQQTVYWSSLSSAVSEVVF 364

Query: 371 SKNATVRLLVSYWTHFVEGTDPYLKSLLYSNILCNSSKFNDCFGKVEIKYYVVPGYNETG 430
           SK+A V++LV+YW HF+  TD YLKSLLYSN+LC SSK+N C GKVEIKYY VPG+N TG
Sbjct: 365 SKHAKVKILVAYWAHFINNTDQYLKSLLYSNVLCYSSKYNKCSGKVEIKYYKVPGFNLTG 424

Query: 431 PALYHNGTRTKNEYPGFSRVQHGKFAVSDVRAHIGTSNLGWDYFYVTSGVSFGTYNPAIV 490
           PA+ HNGT T N YPGF+RV HGK+AVSDVRAHIGTSNL WDYFY T+GVSFGTYNPAIV
Sbjct: 425 PAI-HNGTSTGNIYPGFTRVNHGKYAVSDVRAHIGTSNLVWDYFYSTAGVSFGTYNPAIV 484

Query: 491 KQLQEVFDADWNSPYTLPVKAIQDGPTFSS 521
            QLQE+FDADWNSPY +PV+ +++G   SS
Sbjct: 485 SQLQEIFDADWNSPYAVPVEPLEEGQFHSS 513

BLAST of Cucsa.271500 vs. NCBI nr
Match: gi|778696916|ref|XP_011654227.1| (PREDICTED: phospholipase D Y-like [Cucumis sativus])

HSP 1 Score: 1076.6 bits (2783), Expect = 0.0e+00
Identity = 520/520 (100.00%), Postives = 520/520 (100.00%), Query Frame = 1

Query: 1   MKKAQTPTLTTIFSALFFFFFVSQFPPVLSFTQSQCKGWLVQSIPTDMPQLQLVQGVLST 60
           MKKAQTPTLTTIFSALFFFFFVSQFPPVLSFTQSQCKGWLVQSIPTDMPQLQLVQGVLST
Sbjct: 1   MKKAQTPTLTTIFSALFFFFFVSQFPPVLSFTQSQCKGWLVQSIPTDMPQLQLVQGVLST 60

Query: 61  RDVFVWLAGNSTRRLDIIAQYWELLAGPNDSRSGDYGYSDEDLKKFGANKGYDVYSAIEK 120
           RDVFVWLAGNSTRRLDIIAQYWELLAGPNDSRSGDYGYSDEDLKKFGANKGYDVYSAIEK
Sbjct: 61  RDVFVWLAGNSTRRLDIIAQYWELLAGPNDSRSGDYGYSDEDLKKFGANKGYDVYSAIEK 120

Query: 121 AADRNVNVRLVSHSGVSPNYGKEPADLASGRPNVQNVTLLLGDWYGSGIVHSKVWISDDR 180
           AADRNVNVRLVSHSGVSPNYGKEPADLASGRPNVQNVTLLLGDWYGSGIVHSKVWISDDR
Sbjct: 121 AADRNVNVRLVSHSGVSPNYGKEPADLASGRPNVQNVTLLLGDWYGSGIVHSKVWISDDR 180

Query: 181 DVYIGSANQDWKSLSQVKELGIYLTGCPKIAAHVKVYFENLLKLAFLNRTDYTRTIFDHQ 240
           DVYIGSANQDWKSLSQVKELGIYLTGCPKIAAHVKVYFENLLKLAFLNRTDYTRTIFDHQ
Sbjct: 181 DVYIGSANQDWKSLSQVKELGIYLTGCPKIAAHVKVYFENLLKLAFLNRTDYTRTIFDHQ 240

Query: 241 WQTQRKVPCWSYFIDPESRCRSPLPPYVKFLHTLGYPTISDPYTLNLSIQTPGSRLSTLL 300
           WQTQRKVPCWSYFIDPESRCRSPLPPYVKFLHTLGYPTISDPYTLNLSIQTPGSRLSTLL
Sbjct: 241 WQTQRKVPCWSYFIDPESRCRSPLPPYVKFLHTLGYPTISDPYTLNLSIQTPGSRLSTLL 300

Query: 301 PHSCYLSFAPPELLFGRYQSDEQAWSDTIKSVSSGATVRISTMDWLGQSEFTTPTIYWSS 360
           PHSCYLSFAPPELLFGRYQSDEQAWSDTIKSVSSGATVRISTMDWLGQSEFTTPTIYWSS
Sbjct: 301 PHSCYLSFAPPELLFGRYQSDEQAWSDTIKSVSSGATVRISTMDWLGQSEFTTPTIYWSS 360

Query: 361 LSSAISEVVFSKNATVRLLVSYWTHFVEGTDPYLKSLLYSNILCNSSKFNDCFGKVEIKY 420
           LSSAISEVVFSKNATVRLLVSYWTHFVEGTDPYLKSLLYSNILCNSSKFNDCFGKVEIKY
Sbjct: 361 LSSAISEVVFSKNATVRLLVSYWTHFVEGTDPYLKSLLYSNILCNSSKFNDCFGKVEIKY 420

Query: 421 YVVPGYNETGPALYHNGTRTKNEYPGFSRVQHGKFAVSDVRAHIGTSNLGWDYFYVTSGV 480
           YVVPGYNETGPALYHNGTRTKNEYPGFSRVQHGKFAVSDVRAHIGTSNLGWDYFYVTSGV
Sbjct: 421 YVVPGYNETGPALYHNGTRTKNEYPGFSRVQHGKFAVSDVRAHIGTSNLGWDYFYVTSGV 480

Query: 481 SFGTYNPAIVKQLQEVFDADWNSPYTLPVKAIQDGPTFSS 521
           SFGTYNPAIVKQLQEVFDADWNSPYTLPVKAIQDGPTFSS
Sbjct: 481 SFGTYNPAIVKQLQEVFDADWNSPYTLPVKAIQDGPTFSS 520

BLAST of Cucsa.271500 vs. NCBI nr
Match: gi|659104211|ref|XP_008452848.1| (PREDICTED: phospholipase D Y-like [Cucumis melo])

HSP 1 Score: 1031.6 bits (2666), Expect = 4.9e-298
Identity = 498/521 (95.59%), Postives = 509/521 (97.70%), Query Frame = 1

Query: 1   MKKAQTPTLTTIFSAL-FFFFFVSQFPPVLSFTQSQCKGWLVQSIPTDMPQLQLVQGVLS 60
           MKK  + T+TTIFSAL FFFFF+SQFPPVLSFT+SQCKGWLVQSIPTDMPQLQLVQGVLS
Sbjct: 1   MKKTHSLTVTTIFSALLFFFFFISQFPPVLSFTRSQCKGWLVQSIPTDMPQLQLVQGVLS 60

Query: 61  TRDVFVWLAGNSTRRLDIIAQYWELLAGPNDSRSGDYGYSDEDLKKFGANKGYDVYSAIE 120
           TRDVFVWLAGNSTRRLDIIAQYWELLAGPNDSRSGDYGYS+EDLKKFGANKGYDVYSAIE
Sbjct: 61  TRDVFVWLAGNSTRRLDIIAQYWELLAGPNDSRSGDYGYSNEDLKKFGANKGYDVYSAIE 120

Query: 121 KAADRNVNVRLVSHSGVSPNYGKEPADLASGRPNVQNVTLLLGDWYGSGIVHSKVWISDD 180
           KAADRNVNVRLVSHSGV P +GKEPADLASGRPNVQNVTLLLGDWYGSGIVHSKVWISDD
Sbjct: 121 KAADRNVNVRLVSHSGVYPTFGKEPADLASGRPNVQNVTLLLGDWYGSGIVHSKVWISDD 180

Query: 181 RDVYIGSANQDWKSLSQVKELGIYLTGCPKIAAHVKVYFENLLKLAFLNRTDYTRTIFDH 240
           RDVYIGSANQDWKSLSQVKELGIYLTGCPKIAAHVKVYF+NLLKLAFLN TDYTRTIFDH
Sbjct: 181 RDVYIGSANQDWKSLSQVKELGIYLTGCPKIAAHVKVYFDNLLKLAFLNPTDYTRTIFDH 240

Query: 241 QWQTQRKVPCWSYFIDPESRCRSPLPPYVKFLHTLGYPTISDPYTLNLSIQTPGSRLSTL 300
           QWQTQRKVPCWSYFIDPESRCRSPLPPYVKFLHTLGYP ISDPYTLNLSIQTPGSRLSTL
Sbjct: 241 QWQTQRKVPCWSYFIDPESRCRSPLPPYVKFLHTLGYPIISDPYTLNLSIQTPGSRLSTL 300

Query: 301 LPHSCYLSFAPPELLFGRYQSDEQAWSDTIKSVSSGATVRISTMDWLGQSEFTTPTIYWS 360
           LPHSCYLSFAPPELLFGRYQSDEQAWSDTIKSVS+GATVRISTMDWLGQSEFT PTIYWS
Sbjct: 301 LPHSCYLSFAPPELLFGRYQSDEQAWSDTIKSVSNGATVRISTMDWLGQSEFTNPTIYWS 360

Query: 361 SLSSAISEVVFSKNATVRLLVSYWTHFVEGTDPYLKSLLYSNILCNSSKFNDCFGKVEIK 420
           SLSSAISEVVFSKNATVRLLVSYWTHFVEGTDPYLKSLLYSNILCNSSKFNDCFGKVEIK
Sbjct: 361 SLSSAISEVVFSKNATVRLLVSYWTHFVEGTDPYLKSLLYSNILCNSSKFNDCFGKVEIK 420

Query: 421 YYVVPGYNETGPALYHNGTRTKNEYPGFSRVQHGKFAVSDVRAHIGTSNLGWDYFYVTSG 480
           YYVVPGYNETGPALYHN TRT+N YP F+RVQHGK+AVSDVRAHIGTSNLGWDYFYVTSG
Sbjct: 421 YYVVPGYNETGPALYHNRTRTENRYPSFTRVQHGKYAVSDVRAHIGTSNLGWDYFYVTSG 480

Query: 481 VSFGTYNPAIVKQLQEVFDADWNSPYTLPVKAIQDGPTFSS 521
           VSFGTYNPAIVKQLQEVFDADWNSPYTLPVKAIQDGPTFSS
Sbjct: 481 VSFGTYNPAIVKQLQEVFDADWNSPYTLPVKAIQDGPTFSS 521

BLAST of Cucsa.271500 vs. NCBI nr
Match: gi|747070305|ref|XP_011081968.1| (PREDICTED: phospholipase D Z-like [Sesamum indicum])

HSP 1 Score: 741.9 bits (1914), Expect = 7.7e-211
Identity = 351/521 (67.37%), Postives = 424/521 (81.38%), Query Frame = 1

Query: 1   MKKAQTPTLTTIFSALFFF-FFVSQFPPVLSFTQSQCKGWLVQSIPTDMPQLQLVQGVLS 60
           MK   TP L T++  L +    V       ++   QCK WLVQSIPTDMP L LV GVLS
Sbjct: 1   MKTCATPLLLTLYFLLIYNPTRVVHSSSDGNYDDGQCKAWLVQSIPTDMPHLSLVPGVLS 60

Query: 61  TRDVFVWLAGNSTRRLDIIAQYWELLAGPNDSRSGDYGYSDEDLKKFGANKGYDVYSAIE 120
           T DV  WLAGNS+R LDIIAQYW+L+A P D RSGDYGY+++D+++FGAN+G++V+ A+E
Sbjct: 61  TADVLRWLAGNSSRSLDIIAQYWQLVAHPKDPRSGDYGYTEDDMRRFGANQGFEVHQALE 120

Query: 121 KAADRNVNVRLVSHSGVSPNYGKEPADLASGRPNVQNVTLLLGDWYGSGIVHSKVWISDD 180
            AADRN+++RL+ HSGV P+Y +EP+ LASGRPNV+NVTLLL DW+GSGIVH+KVWISD 
Sbjct: 121 NAADRNISIRLLQHSGVYPDYTEEPSALASGRPNVKNVTLLLKDWWGSGIVHAKVWISDS 180

Query: 181 RDVYIGSANQDWKSLSQVKELGIYLTGCPKIAAHVKVYFENLLKLAFLNRTDYTRTIFDH 240
           +DVYIGSAN DWKSL+QVKE+GIYL GCP+IA +V++YFENL KLA LN ++YT +I+D 
Sbjct: 181 QDVYIGSANNDWKSLTQVKEVGIYLVGCPRIAENVEIYFENLWKLAHLNSSNYTESIWDQ 240

Query: 241 QWQTQRKVPCWSYFIDPESRCRSPLPPYVKFLHTLGYPTISDPYTLNLSIQTPGSRLSTL 300
           QWQ  RKVPCWS+F+ P+ RCRSPLP YVK  H  GYP +SDP   ++SI+TPG   ST 
Sbjct: 241 QWQIVRKVPCWSHFLHPKERCRSPLPKYVKVKHVTGYPVLSDPDIFHVSIETPGRNYSTS 300

Query: 301 LPHSCYLSFAPPELLFGRYQSDEQAWSDTIKSVSSGATVRISTMDWLGQSEFTTPTIYWS 360
              SCYLSFAPPELLFG+YQ+DEQAW DTIKSV +GAT+RISTMDWLGQS++T+ T+YWS
Sbjct: 301 QHQSCYLSFAPPELLFGKYQTDEQAWVDTIKSVGNGATLRISTMDWLGQSQYTSQTVYWS 360

Query: 361 SLSSAISEVVFSKNATVRLLVSYWTHFVEGTDPYLKSLLYSNILCNSSKFNDCFGKVEIK 420
           SLSSAISEVVFSK A V+LLV+YW HF+  TD YLKSLLY+N LC+SSK+N C GKVEIK
Sbjct: 361 SLSSAISEVVFSKQAKVKLLVAYWAHFINNTDVYLKSLLYTNNLCSSSKYNKCHGKVEIK 420

Query: 421 YYVVPGYNETGPALYHNGTRTKNEYPGFSRVQHGKFAVSDVRAHIGTSNLGWDYFYVTSG 480
           YY+VPG+N TGPA   NGT T N YPG++RV HGK+AVSDVRAHIGTSNL WDYFY T+G
Sbjct: 421 YYMVPGFNLTGPAT-QNGTATGNIYPGYTRVNHGKYAVSDVRAHIGTSNLIWDYFYSTAG 480

Query: 481 VSFGTYNPAIVKQLQEVFDADWNSPYTLPVKAIQDGPTFSS 521
           VSFGTYN AIV QLQE+FDADW+SPY +PV+ ++DG  FSS
Sbjct: 481 VSFGTYNSAIVSQLQEIFDADWDSPYAVPVEPLEDGHAFSS 520

BLAST of Cucsa.271500 vs. NCBI nr
Match: gi|643724311|gb|KDP33512.1| (hypothetical protein JCGZ_07083 [Jatropha curcas])

HSP 1 Score: 729.6 bits (1882), Expect = 4.0e-207
Identity = 345/501 (68.86%), Postives = 409/501 (81.64%), Query Frame = 1

Query: 20  FFVSQFPPVLSFTQSQCKGWLVQSIPTDMPQLQLVQGVLSTRDVFVWLAGNSTRRLDIIA 79
           FF++Q         S+CK WLVQSIPTDMPQL+ V GVL+T DV  WLAGNST+ LDIIA
Sbjct: 20  FFITQ-------ADSKCKAWLVQSIPTDMPQLRRVPGVLTTGDVLRWLAGNSTKGLDIIA 79

Query: 80  QYWELLAGPNDSRSGDYGYSDEDLKKFGANKGYDVYSAIEKAADRNVNVRLVSHSGVSPN 139
           QYW+L+A   D RSGDYGYS  D++KFGA +G+ VY AIE AADRNV++RL+ HSGV P+
Sbjct: 80  QYWQLIANSKDPRSGDYGYSKSDMEKFGAFEGFGVYKAIENAADRNVSIRLLQHSGVYPD 139

Query: 140 YGKEPADLASGRPNVQNVTLLLGDWYGSGIVHSKVWISDDRDVYIGSANQDWKSLSQVKE 199
           + KEP DLASGR NV NVTLLLG+W+GSGIVH+KVWISD RDVYIGSAN DWKSL+QVKE
Sbjct: 140 FTKEPNDLASGRLNVDNVTLLLGEWWGSGIVHAKVWISDSRDVYIGSANNDWKSLTQVKE 199

Query: 200 LGIYLTGCPKIAAHVKVYFENLLKLAFLNRTDYTRTIFDHQWQTQRKVPCWSYFIDPESR 259
           +GIYL GC KI   V+ YFENL KLA LN + YTR+++D QWQ  R VPCWS+FID ++R
Sbjct: 200 VGIYLVGCKKIVGKVETYFENLWKLAHLNSSAYTRSVWDQQWQINRTVPCWSHFIDSKAR 259

Query: 260 CRSPLPPYVKFLHTLGYPTISDPYTLNLSIQTPGSRLSTLLPHSCYLSFAPPELLFGRYQ 319
           CRSPLP +V+  H  GYP +SDP+ + + I+TPG R STL P+  YLSFAPPEL FG+YQ
Sbjct: 260 CRSPLPTFVEVPHVAGYPVLSDPHMIKVPIKTPGQRFSTLEPYLSYLSFAPPELSFGKYQ 319

Query: 320 SDEQAWSDTIKSVSSGATVRISTMDWLGQSEFTTPTIYWSSLSSAISEVVFSKNATVRLL 379
           +DEQ W DTIKSV SG+TVRI+TMDWLGQS+FT   +YWSSLSSAISEV+FSK+ATV++L
Sbjct: 320 TDEQGWIDTIKSVGSGSTVRINTMDWLGQSQFTKEKVYWSSLSSAISEVIFSKHATVKVL 379

Query: 380 VSYWTHFVEGTDPYLKSLLYSNILCNSSKFNDCFGKVEIKYYVVPGYNETGPALYHNGTR 439
           V+YW HF++ TD YLKSLLYS+ILCNSSK+N C GKVEIKYYVVPGYN TGPA   NG R
Sbjct: 380 VAYWAHFIDNTDQYLKSLLYSDILCNSSKYNKCHGKVEIKYYVVPGYNLTGPAT-RNGKR 439

Query: 440 TKNEYPGFSRVQHGKFAVSDVRAHIGTSNLGWDYFYVTSGVSFGTYNPAIVKQLQEVFDA 499
           T N+YP F+RV HGK+AVSDVRAHIGTSNL WDYFY T+GVSFGTYNPAIV QLQ++FDA
Sbjct: 440 TGNKYPAFTRVNHGKYAVSDVRAHIGTSNLVWDYFYTTAGVSFGTYNPAIVLQLQKIFDA 499

Query: 500 DWNSPYTLPVKAIQDGPTFSS 521
           DW+SPY +P + ++ G  FSS
Sbjct: 500 DWDSPYAVPFEGLEAGHAFSS 512

BLAST of Cucsa.271500 vs. NCBI nr
Match: gi|802626330|ref|XP_012076426.1| (PREDICTED: phospholipase D Z-like [Jatropha curcas])

HSP 1 Score: 729.6 bits (1882), Expect = 4.0e-207
Identity = 345/501 (68.86%), Postives = 409/501 (81.64%), Query Frame = 1

Query: 20  FFVSQFPPVLSFTQSQCKGWLVQSIPTDMPQLQLVQGVLSTRDVFVWLAGNSTRRLDIIA 79
           FF++Q         S+CK WLVQSIPTDMPQL+ V GVL+T DV  WLAGNST+ LDIIA
Sbjct: 23  FFITQ-------ADSKCKAWLVQSIPTDMPQLRRVPGVLTTGDVLRWLAGNSTKGLDIIA 82

Query: 80  QYWELLAGPNDSRSGDYGYSDEDLKKFGANKGYDVYSAIEKAADRNVNVRLVSHSGVSPN 139
           QYW+L+A   D RSGDYGYS  D++KFGA +G+ VY AIE AADRNV++RL+ HSGV P+
Sbjct: 83  QYWQLIANSKDPRSGDYGYSKSDMEKFGAFEGFGVYKAIENAADRNVSIRLLQHSGVYPD 142

Query: 140 YGKEPADLASGRPNVQNVTLLLGDWYGSGIVHSKVWISDDRDVYIGSANQDWKSLSQVKE 199
           + KEP DLASGR NV NVTLLLG+W+GSGIVH+KVWISD RDVYIGSAN DWKSL+QVKE
Sbjct: 143 FTKEPNDLASGRLNVDNVTLLLGEWWGSGIVHAKVWISDSRDVYIGSANNDWKSLTQVKE 202

Query: 200 LGIYLTGCPKIAAHVKVYFENLLKLAFLNRTDYTRTIFDHQWQTQRKVPCWSYFIDPESR 259
           +GIYL GC KI   V+ YFENL KLA LN + YTR+++D QWQ  R VPCWS+FID ++R
Sbjct: 203 VGIYLVGCKKIVGKVETYFENLWKLAHLNSSAYTRSVWDQQWQINRTVPCWSHFIDSKAR 262

Query: 260 CRSPLPPYVKFLHTLGYPTISDPYTLNLSIQTPGSRLSTLLPHSCYLSFAPPELLFGRYQ 319
           CRSPLP +V+  H  GYP +SDP+ + + I+TPG R STL P+  YLSFAPPEL FG+YQ
Sbjct: 263 CRSPLPTFVEVPHVAGYPVLSDPHMIKVPIKTPGQRFSTLEPYLSYLSFAPPELSFGKYQ 322

Query: 320 SDEQAWSDTIKSVSSGATVRISTMDWLGQSEFTTPTIYWSSLSSAISEVVFSKNATVRLL 379
           +DEQ W DTIKSV SG+TVRI+TMDWLGQS+FT   +YWSSLSSAISEV+FSK+ATV++L
Sbjct: 323 TDEQGWIDTIKSVGSGSTVRINTMDWLGQSQFTKEKVYWSSLSSAISEVIFSKHATVKVL 382

Query: 380 VSYWTHFVEGTDPYLKSLLYSNILCNSSKFNDCFGKVEIKYYVVPGYNETGPALYHNGTR 439
           V+YW HF++ TD YLKSLLYS+ILCNSSK+N C GKVEIKYYVVPGYN TGPA   NG R
Sbjct: 383 VAYWAHFIDNTDQYLKSLLYSDILCNSSKYNKCHGKVEIKYYVVPGYNLTGPAT-RNGKR 442

Query: 440 TKNEYPGFSRVQHGKFAVSDVRAHIGTSNLGWDYFYVTSGVSFGTYNPAIVKQLQEVFDA 499
           T N+YP F+RV HGK+AVSDVRAHIGTSNL WDYFY T+GVSFGTYNPAIV QLQ++FDA
Sbjct: 443 TGNKYPAFTRVNHGKYAVSDVRAHIGTSNLVWDYFYTTAGVSFGTYNPAIVLQLQKIFDA 502

Query: 500 DWNSPYTLPVKAIQDGPTFSS 521
           DW+SPY +P + ++ G  FSS
Sbjct: 503 DWDSPYAVPFEGLEAGHAFSS 515

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
PLDY_DICDI1.6e-4628.00Phospholipase D Y OS=Dictyostelium discoideum GN=pldY PE=2 SV=1[more]
PLD3_PONAB2.9e-3225.33Phospholipase D3 OS=Pongo abelii GN=PLD3 PE=2 SV=1[more]
PLD3_HUMAN2.9e-3225.67Phospholipase D3 OS=Homo sapiens GN=PLD3 PE=1 SV=1[more]
PLD3_MACFA2.9e-3225.14Phospholipase D3 OS=Macaca fascicularis GN=PLD3 PE=2 SV=1[more]
K4_VACCW4.3e-2826.89Phospholipase-D-like protein K4 OS=Vaccinia virus (strain Western Reserve) GN=VA... [more]
Match NameE-valueIdentityDescription
A0A0A0L498_CUCSA0.0e+00100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_4G651930 PE=4 SV=1[more]
A0A067KBJ9_JATCU2.8e-20768.86Uncharacterized protein OS=Jatropha curcas GN=JCGZ_07083 PE=4 SV=1[more]
V4UMB2_9ROSI2.8e-20766.34Uncharacterized protein OS=Citrus clementina GN=CICLE_v10008002mg PE=4 SV=1[more]
A0A067FW62_CITSI2.8e-20766.34Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g009992mg PE=4 SV=1[more]
D7SV42_VITVI1.2e-20567.25Putative uncharacterized protein OS=Vitis vinifera GN=VIT_04s0008g06620 PE=4 SV=... [more]
Match NameE-valueIdentityDescription
gi|778696916|ref|XP_011654227.1|0.0e+00100.00PREDICTED: phospholipase D Y-like [Cucumis sativus][more]
gi|659104211|ref|XP_008452848.1|4.9e-29895.59PREDICTED: phospholipase D Y-like [Cucumis melo][more]
gi|747070305|ref|XP_011081968.1|7.7e-21167.37PREDICTED: phospholipase D Z-like [Sesamum indicum][more]
gi|643724311|gb|KDP33512.1|4.0e-20768.86hypothetical protein JCGZ_07083 [Jatropha curcas][more]
gi|802626330|ref|XP_012076426.1|4.0e-20768.86PREDICTED: phospholipase D Z-like [Jatropha curcas][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR001736PLipase_D/transphosphatidylase
IPR025202PLD-like_dom
Vocabulary: Molecular Function
TermDefinition
GO:0003824catalytic activity
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008152 metabolic process
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0003824 catalytic activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsa.271500.1Cucsa.271500.1mRNA


Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001736Phospholipase D/TransphosphatidylaseSMARTSM00155pld_4coord: 166..193
score: 2.7E-5coord: 447..473
score: 0.
IPR001736Phospholipase D/TransphosphatidylasePROFILEPS50035PLDcoord: 166..193
score: 12.511coord: 447..473
score:
IPR025202Phospholipase D-like domainPFAMPF13091PLDc_2coord: 111..221
score: 2.6
NoneNo IPR availableGENE3DG3DSA:3.30.870.10coord: 337..409
score: 9.5E-14coord: 448..508
score: 9.5E-14coord: 52..223
score: 5.1
NoneNo IPR availablePANTHERPTHR10185PHOSPHOLIPASE D - RELATEDcoord: 246..270
score: 3.5E-155coord: 300..515
score: 3.5E-155coord: 33..229
score: 3.5E
NoneNo IPR availablePANTHERPTHR10185:SF17PHOSPHOLIPASE D F09G2.8-RELATEDcoord: 246..270
score: 3.5E-155coord: 300..515
score: 3.5E-155coord: 33..229
score: 3.5E
NoneNo IPR availableunknownSSF56024Phospholipase D/nucleasecoord: 111..224
score: 1.91E-18coord: 58..83
score: 1.91E-18coord: 307..513
score: 1.7

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Cucsa.271500Silver-seed gourdcarcgyB0173
Cucsa.271500Silver-seed gourdcarcgyB0584
Cucsa.271500Silver-seed gourdcarcgyB0875
Cucsa.271500Silver-seed gourdcarcgyB1095
Cucsa.271500Cucumber (Chinese Long) v3cgycucB413
Cucsa.271500Cucumber (Chinese Long) v3cgycucB414
Cucsa.271500Cucumber (Chinese Long) v3cgycucB417
Cucsa.271500Watermelon (97103) v2cgywmbB473
Cucsa.271500Watermelon (97103) v2cgywmbB474
Cucsa.271500Watermelon (97103) v2cgywmbB475
Cucsa.271500Wax gourdcgywgoB548
Cucsa.271500Wax gourdcgywgoB551
Cucsa.271500Cucumber (Gy14) v1cgycgyB054
Cucsa.271500Cucumber (Gy14) v1cgycgyB099
Cucsa.271500Cucurbita maxima (Rimu)cgycmaB0752
Cucsa.271500Cucurbita maxima (Rimu)cgycmaB0753
Cucsa.271500Cucurbita maxima (Rimu)cgycmaB0757
Cucsa.271500Cucurbita maxima (Rimu)cgycmaB0758
Cucsa.271500Cucurbita moschata (Rifu)cgycmoB0748
Cucsa.271500Cucurbita moschata (Rifu)cgycmoB0743
Cucsa.271500Cucurbita moschata (Rifu)cgycmoB0744
Cucsa.271500Cucurbita moschata (Rifu)cgycmoB0745
Cucsa.271500Cucurbita moschata (Rifu)cgycmoB0749
Cucsa.271500Wild cucumber (PI 183967)cgycpiB407
Cucsa.271500Wild cucumber (PI 183967)cgycpiB408
Cucsa.271500Wild cucumber (PI 183967)cgycpiB410
Cucsa.271500Cucumber (Chinese Long) v2cgycuB386
Cucsa.271500Cucumber (Chinese Long) v2cgycuB388
Cucsa.271500Melon (DHL92) v3.5.1cgymeB451
Cucsa.271500Melon (DHL92) v3.5.1cgymeB452
Cucsa.271500Melon (DHL92) v3.5.1cgymeB453
Cucsa.271500Watermelon (Charleston Gray)cgywcgB468
Cucsa.271500Watermelon (Charleston Gray)cgywcgB472
Cucsa.271500Watermelon (Charleston Gray)cgywcgB473
Cucsa.271500Watermelon (97103) v1cgywmB495
Cucsa.271500Watermelon (97103) v1cgywmB496
Cucsa.271500Watermelon (97103) v1cgywmB501
Cucsa.271500Cucurbita pepo (Zucchini)cgycpeB0707
Cucsa.271500Cucurbita pepo (Zucchini)cgycpeB0710
Cucsa.271500Cucurbita pepo (Zucchini)cgycpeB0713
Cucsa.271500Bottle gourd (USVL1VR-Ls)cgylsiB439
Cucsa.271500Bottle gourd (USVL1VR-Ls)cgylsiB440
Cucsa.271500Bottle gourd (USVL1VR-Ls)cgylsiB441
Cucsa.271500Melon (DHL92) v3.6.1cgymedB447
Cucsa.271500Melon (DHL92) v3.6.1cgymedB448
Cucsa.271500Melon (DHL92) v3.6.1cgymedB449