Cucsa.271500.1 (mRNA) Cucumber (Gy14) v1

NameCucsa.271500.1
TypemRNA
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionPhospholipase D Z
Locationscaffold02500 : 338784 .. 342792 (+)
Sequence length1686
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGAAAGCTCAAACTCCAACTTTAACTACCATTTTCAGTGCTCTCTTCTTCTTCTTCTTCGTCAGCCAATTCCCGCCTGTTCTCTCCTTCACTCAATCACAATGCAAGGGTTGGCTTGTACAATCAATTCCCACAGATATGCCGCAGCTTCAGCTGGTTCAAGGCGTCCTCTCGACTCGTAAATCTAACACTCATTTTTCCATTTGCATTTCAAATTCATTTGATTCTTTTGCTTATTTCTTTGCTCATCTGTTTAGATTCCTAGAAACTTTGTCCTAATTCTTAGATTTGTTGGAATATCAGGAGATGTATTCGTATGGTTAGCTGGAAACTCGACGCGGCGTTTGGATATCATTGCTCAGTACTGGGAATTACTAGCTGGTCCTAATGATTCTCGATCAGGGGACTATGGATACTCGGATGAGGATTTGAAGAAATTTGGGGCTAATAAAGGTTATGATGTTTATTCAGCCATAGAGAAAGCTGCCGATCGCAATGTCAATGTCAGGTACATTGAATCTGGGTTTGGTCGATGGTGTTGCTTTGATAACAGTATAGGGTTCGGAAATGGAAATGGTTTAAACCCAAATGGCAAACTGCTCTGGAGTTGACCTTACAAACTGTAGTATTAAATTACATTTGGTAGTTCAAAATATGCGTATTTTACTCCTTGAAGAGGATAGGATCAATATGTAAGTTTTAAGATTTTTAATTGCTTATCCATTTGAATGGTGAAACAATTTCTGGTTATGAACTATAAGCAGACATTTTCAAGAAGAAGATATGATATTGCCAAAATAAGCTTAACTCAACGGTATTGGTATGATCTCCATACCAAGGGGTTGGAAGTTCATTCCCCCGCTCCGTGAACTCAAAAAAaGAAAAAGAAGAAAGAAAAGAAAAAAGAAGATATGAGTAATTCTTGGGAATGCAGCTAAAGTTTCAGATCTATTAGATAGACAACTTTTGATTTGAAACGAGTTGGAAAAAACCTCCCACTTTATGGATAAGAAAAATGACCGTAGGTGAAACATAATAAATATATAATCTTGAAATTCTTTATTATTTTTATTAATTTGAGAACTTTTAATTATGTAGGCTGGTATCTCACTCGGGGGTATCTCCAAACTATGGAAAAGAACCAGCTGACTTAGCATCTGGAAGGCCAAACGTGCAGAATGTAACTTTATTACTTGGTGATTGGTATGGATCAGGCATAGTCCACTCCAAAGTTTGGATATCTGATGATAGAGATGTGTATATAGGGTCTGCAAACCAGGACTGGAAATCTCTTTCACAGGTAAGCTTTTATTGGAAGGATTTTTTATATCAAGAACTTACCTTCAATCACATTGTTTATCGATGAGGATTGAGGATGGTTTAAAACAGATGGAGCTCCAAATCTTTGATACTGGTTTTTATTTATTGGTGAAATAATTCTGACTTCCAGGGTATCCTTTTCGAACGTTTTTGTTGAATTCTTTGCTTTTCTATAGTTGGTCACACAAACACAACGAAATTGAATCTTAAGGTTGATGCTCCTATCACTTGATGGTTTTAGGTGAAGGAACTTGGAATATATCTTACAGGCTGTCCAAAAATAGCAGCTCATGTCAAGGTCTATTTTGAAAATTTATTGAAACTTGCATTTCTTAATCGCACGGATTACACAAGAACAATATTTGACCACCAGTGGCAAACACAAAGAAAAGTTCCTTGCTGGTCATATTTTATTGATCCCGAGTCTAGATGCAGGCAAGTCCAACCATTCTCGTCATCCTTTGTTTTTCAGAAGAATGTACTTTTGTGATTTATTCGGTATTGGTAGTAGATTTCTTTTTtCTTCCTTTTTTtCTGAGTTGTTGGATTTAGGTAACTGAAGACCTCTTTTTCTAGCTCCTGAAGGAATGCATACATCACGAGCCACTTATCAAGAACATTGCATGGTCCTTTTAATAATTGTAATCTATCATCCCATGCATCGATGTTGGAATCCTTAAAAaCTTATCTATACACTCATGTCTGACGTTTCCAATATGTTCACTGTTAACAATTTTGCAGGTCACCTCTTCCACCATATGTTAAGTTTCTCCATACGTTGGGCTATCCGACTATATCAGATCCTTACACACTCAATTTATCAATCCAGACTCCTGGTAGCCGCTTATCAACGTTGCTGCCTCACTCTTGCTATCTCTCTTTCGCCCCTCCAGAGGTATTTGACAATTCTGCCACCTTTTTATGCTATTTCTCTTCTGCAATACAAAGTTTAAACTAAACTAAGGGCAATGCGTAAGGGGAAAAAGAACAAAATCTGAGTGAGCAGAGTCATGCTATAAAAATCTAGTTAGTAATGTATTATAATTTCATTATTACGAGCATGATACATGCAGATTTAAGGGAGATTGTATATAACCACGTACTAAATTGAACAATCTTATCAAATTAAAATTGAACTGATATAGTGCTTGAGAGGTGAATTGAATTAATTGATATCCCTAAGTTCTTTTTATGGAATATTAGTTCAACATACAACTTTAGGTGCGAACCCAATTTACTATAGTGAATTGTATCATTTTGTTATGAATTACTTCAACTTAATTTCAAGTGAAATGTTTACTTTCTGGACTTTTGTCAGATTTTTATGTCAATTTGGCATCCTATCCTATGAACCCGTACTTCCTTGCTGACAGCGTTTACCATTTGTTGCCGTGTTTACTATTTCTTACTTATCCTCTTTTTCCTTCTCTATTATCGTGTTTACTATTTCCTATCCAAACTAAAATAGTTTGCATCCCTATCATAACTCAAAGACTGACAATCATTGGCTATAATATTAAATTGTCACCCCAAACACCCATAGTTTATATATAGTGCCTAATTTTATCCCCCAATTAAAATTCATTTGCAGTCTACAGCTATTATTTGGCAGGTACCAATCTGATGAACAGGCATGGTCAGACACAATTAAATCTGTGAGCAGTGGAGCAACTGTTAGGATTAGTACCATGGACTGGCTGGGTCAATCTGAATTTACCACACCCACAATTTACTGGTCGTCCTTGTCTTCTGCAATATCGGAGGTAAATTTCTACGTGACTTTTCCTGTCTGCTTCTGTGGTTAATTTTTCTTTTTTtAAACGTTCAAGGAACTATTAACCATAAATTTAAAAGTCAAGGAGTAGACTTAGGTTGTTGATCTTACAAAAATATTTAACAGACGGAAAGATACACAATAGTTAAGCACTCTTGAGATAACTGATGTTAAGTACATCCAAATCATGTGAGGCCGCCTGGATCTTCTACATCCTAACTACAACCCTTTCAAGTTTCAATACCGAGGTCATCTTGTTTGTACAATCTGGTAGAAAATGGATTACATTCTAGTTTTTAAATATTAACAAAACCATTTTATGTATAGGTCGTCTTTTCAAAAAATGCAACTGTCAGGTTATTGGTATCATACTGGACACATTTTGTCGAGGGCACTGATCCATACCTCAAGTCTCTCCTCTACTCAAATATCCTCTGTAACTCTTCAAAGTTCAACGATTGTTTTGGGAAAGTTGAGATCAAATACTATGTGGTTCCCGGATACAACGAAACAGGACCAGCACTTTATCACAACGGAACAAGAACGAAAAACGAATACCCAGGTTTTAGTAGAGTTCAACATGGAAAGTTTGCAGTTAGTGACGTTAGAGCACATATAGGAACAAGCAATCTTGGATGGGATTACTTTTATGTAACTTCTGGTGTCAGCTTTGGAACATACAACCCAGCTATTGTTAAGCAGCTTCAAGAAGTGTTTGATGCTGACTGGAATTCTCCTTATACTCTTCCTGTTAAAGCCATACAGGATGGCCCTACATTTTCCAGCTGATTTGCACTTGAATCAATAGAAGTTCGATGAAATTACAAAGGAACATGCTACAAATCATGTGGCTTATTATTTAGTACATCTCTACACTAGATTGGAAGTGTTACAGATTCAGTCTAGATGTGT

mRNA sequence

ATGAAGAAAGCTCAAACTCCAACTTTAACTACCATTTTCAGTGCTCTCTTCTTCTTCTTCTTCGTCAGCCAATTCCCGCCTGTTCTCTCCTTCACTCAATCACAATGCAAGGGTTGGCTTGTACAATCAATTCCCACAGATATGCCGCAGCTTCAGCTGGTTCAAGGCGTCCTCTCGACTCGAGATGTATTCGTATGGTTAGCTGGAAACTCGACGCGGCGTTTGGATATCATTGCTCAGTACTGGGAATTACTAGCTGGTCCTAATGATTCTCGATCAGGGGACTATGGATACTCGGATGAGGATTTGAAGAAATTTGGGGCTAATAAAGGTTATGATGTTTATTCAGCCATAGAGAAAGCTGCCGATCGCAATGTCAATGTCAGGCTGGTATCTCACTCGGGGGTATCTCCAAACTATGGAAAAGAACCAGCTGACTTAGCATCTGGAAGGCCAAACGTGCAGAATGTAACTTTATTACTTGGTGATTGGTATGGATCAGGCATAGTCCACTCCAAAGTTTGGATATCTGATGATAGAGATGTGTATATAGGGTCTGCAAACCAGGACTGGAAATCTCTTTCACAGGTGAAGGAACTTGGAATATATCTTACAGGCTGTCCAAAAATAGCAGCTCATGTCAAGGTCTATTTTGAAAATTTATTGAAACTTGCATTTCTTAATCGCACGGATTACACAAGAACAATATTTGACCACCAGTGGCAAACACAAAGAAAAGTTCCTTGCTGGTCATATTTTATTGATCCCGAGTCTAGATGCAGGTCACCTCTTCCACCATATGTTAAGTTTCTCCATACGTTGGGCTATCCGACTATATCAGATCCTTACACACTCAATTTATCAATCCAGACTCCTGGTAGCCGCTTATCAACGTTGCTGCCTCACTCTTGCTATCTCTCTTTCGCCCCTCCAGAGCTATTATTTGGCAGGTACCAATCTGATGAACAGGCATGGTCAGACACAATTAAATCTGTGAGCAGTGGAGCAACTGTTAGGATTAGTACCATGGACTGGCTGGGTCAATCTGAATTTACCACACCCACAATTTACTGGTCGTCCTTGTCTTCTGCAATATCGGAGGTCGTCTTTTCAAAAAATGCAACTGTCAGGTTATTGGTATCATACTGGACACATTTTGTCGAGGGCACTGATCCATACCTCAAGTCTCTCCTCTACTCAAATATCCTCTGTAACTCTTCAAAGTTCAACGATTGTTTTGGGAAAGTTGAGATCAAATACTATGTGGTTCCCGGATACAACGAAACAGGACCAGCACTTTATCACAACGGAACAAGAACGAAAAACGAATACCCAGGTTTTAGTAGAGTTCAACATGGAAAGTTTGCAGTTAGTGACGTTAGAGCACATATAGGAACAAGCAATCTTGGATGGGATTACTTTTATGTAACTTCTGGTGTCAGCTTTGGAACATACAACCCAGCTATTGTTAAGCAGCTTCAAGAAGTGTTTGATGCTGACTGGAATTCTCCTTATACTCTTCCTGTTAAAGCCATACAGGATGGCCCTACATTTTCCAGCTGATTTGCACTTGAATCAATAGAAGTTCGATGAAATTACAAAGGAACATGCTACAAATCATGTGGCTTATTATTTAGTACATCTCTACACTAGATTGGAAGTGTTACAGATTCAGTCTAGATGTGT

Coding sequence (CDS)

ATGAAGAAAGCTCAAACTCCAACTTTAACTACCATTTTCAGTGCTCTCTTCTTCTTCTTCTTCGTCAGCCAATTCCCGCCTGTTCTCTCCTTCACTCAATCACAATGCAAGGGTTGGCTTGTACAATCAATTCCCACAGATATGCCGCAGCTTCAGCTGGTTCAAGGCGTCCTCTCGACTCGAGATGTATTCGTATGGTTAGCTGGAAACTCGACGCGGCGTTTGGATATCATTGCTCAGTACTGGGAATTACTAGCTGGTCCTAATGATTCTCGATCAGGGGACTATGGATACTCGGATGAGGATTTGAAGAAATTTGGGGCTAATAAAGGTTATGATGTTTATTCAGCCATAGAGAAAGCTGCCGATCGCAATGTCAATGTCAGGCTGGTATCTCACTCGGGGGTATCTCCAAACTATGGAAAAGAACCAGCTGACTTAGCATCTGGAAGGCCAAACGTGCAGAATGTAACTTTATTACTTGGTGATTGGTATGGATCAGGCATAGTCCACTCCAAAGTTTGGATATCTGATGATAGAGATGTGTATATAGGGTCTGCAAACCAGGACTGGAAATCTCTTTCACAGGTGAAGGAACTTGGAATATATCTTACAGGCTGTCCAAAAATAGCAGCTCATGTCAAGGTCTATTTTGAAAATTTATTGAAACTTGCATTTCTTAATCGCACGGATTACACAAGAACAATATTTGACCACCAGTGGCAAACACAAAGAAAAGTTCCTTGCTGGTCATATTTTATTGATCCCGAGTCTAGATGCAGGTCACCTCTTCCACCATATGTTAAGTTTCTCCATACGTTGGGCTATCCGACTATATCAGATCCTTACACACTCAATTTATCAATCCAGACTCCTGGTAGCCGCTTATCAACGTTGCTGCCTCACTCTTGCTATCTCTCTTTCGCCCCTCCAGAGCTATTATTTGGCAGGTACCAATCTGATGAACAGGCATGGTCAGACACAATTAAATCTGTGAGCAGTGGAGCAACTGTTAGGATTAGTACCATGGACTGGCTGGGTCAATCTGAATTTACCACACCCACAATTTACTGGTCGTCCTTGTCTTCTGCAATATCGGAGGTCGTCTTTTCAAAAAATGCAACTGTCAGGTTATTGGTATCATACTGGACACATTTTGTCGAGGGCACTGATCCATACCTCAAGTCTCTCCTCTACTCAAATATCCTCTGTAACTCTTCAAAGTTCAACGATTGTTTTGGGAAAGTTGAGATCAAATACTATGTGGTTCCCGGATACAACGAAACAGGACCAGCACTTTATCACAACGGAACAAGAACGAAAAACGAATACCCAGGTTTTAGTAGAGTTCAACATGGAAAGTTTGCAGTTAGTGACGTTAGAGCACATATAGGAACAAGCAATCTTGGATGGGATTACTTTTATGTAACTTCTGGTGTCAGCTTTGGAACATACAACCCAGCTATTGTTAAGCAGCTTCAAGAAGTGTTTGATGCTGACTGGAATTCTCCTTATACTCTTCCTGTTAAAGCCATACAGGATGGCCCTACATTTTCCAGCTGA

Protein sequence

MKKAQTPTLTTIFSALFFFFFVSQFPPVLSFTQSQCKGWLVQSIPTDMPQLQLVQGVLSTRDVFVWLAGNSTRRLDIIAQYWELLAGPNDSRSGDYGYSDEDLKKFGANKGYDVYSAIEKAADRNVNVRLVSHSGVSPNYGKEPADLASGRPNVQNVTLLLGDWYGSGIVHSKVWISDDRDVYIGSANQDWKSLSQVKELGIYLTGCPKIAAHVKVYFENLLKLAFLNRTDYTRTIFDHQWQTQRKVPCWSYFIDPESRCRSPLPPYVKFLHTLGYPTISDPYTLNLSIQTPGSRLSTLLPHSCYLSFAPPELLFGRYQSDEQAWSDTIKSVSSGATVRISTMDWLGQSEFTTPTIYWSSLSSAISEVVFSKNATVRLLVSYWTHFVEGTDPYLKSLLYSNILCNSSKFNDCFGKVEIKYYVVPGYNETGPALYHNGTRTKNEYPGFSRVQHGKFAVSDVRAHIGTSNLGWDYFYVTSGVSFGTYNPAIVKQLQEVFDADWNSPYTLPVKAIQDGPTFSS*
BLAST of Cucsa.271500.1 vs. Swiss-Prot
Match: PLDY_DICDI (Phospholipase D Y OS=Dictyostelium discoideum GN=pldY PE=2 SV=1)

HSP 1 Score: 188.7 bits (478), Expect = 1.6e-46
Identity = 140/500 (28.00%), Postives = 241/500 (48.20%), Query Frame = 1

Query: 16  LFFFFFVSQFPPVLS-FTQSQCKGWLVQSIPTDMPQLQLVQGVLSTRDVFVWLAGNSTRR 75
           +   FFV+    ++S  T + C+G  +Q I   +P    +   LST D ++ L  N+   
Sbjct: 9   IIVLFFVNVNSKIISESTLNSCQGGTIQ-IAESIPLGLDISTNLSTHDAWMDLITNAQES 68

Query: 76  LDIIAQYWELLAGPNDSRSGDYGYSDEDLKKFGANKGYDVYSAIEKAADRNVNVRLVSHS 135
           +D+   Y+ LL G           SD D   +G   G D+++AI +A  R +NVR+V + 
Sbjct: 69  IDLGFFYFTLLGG-----------SDLD-PVYGGQLGIDIFNAIVEAHSRGINVRIVQNE 128

Query: 136 GVSPNYGKEPADLASGRPNVQNVTLLLGDWY---GSGIVHSKVWISDDRDVYIGSANQDW 195
                   E   LA     V+++     DW    GSG++H+K+ I D+   Y+GSAN DW
Sbjct: 129 PSESFPDTETQTLAKLGIQVRSI-----DWVSLVGSGVLHTKLIIIDESSAYVGSANADW 188

Query: 196 KSLSQVKELGIYLTGCPKIAAHVKVYFENLLKLAFLNRTDYTRTIFDHQWQTQRKVPCWS 255
            SL+QVKELGI L  CP + A  ++ F+      + N  D+T ++  + W +  +    +
Sbjct: 189 SSLAQVKELGIVLKNCPTMVADTEIAFQQ-----YWNAADFT-SLPINDWGSNYQ----A 248

Query: 256 YFIDPESRCRSPLPPYVKFLHTLGYPTISDPYTLNLSIQTPGSRLSTLLPHSCYLSFAPP 315
            F +               + +L     ++    N +    GS  + +  +  +L+ +PP
Sbjct: 249 LFNNTN-------------MASLSLNNNNNNNNKNKNNDNDGSGTNQM--YEMFLAVSPP 308

Query: 316 ELLFGRYQSDEQAWSDTIKSVSSGATVRISTMDWLGQSEFTTPTIYWSSLSSAISEVVFS 375
           +        D  A  D I +     ++ I+ MD+   + +  P  YW  + +A+    F+
Sbjct: 309 QFQSTYRTGDIDALMDAINNADQ--SICITVMDYTPTTLYNDPNTYWPLIDNALRAAAFN 368

Query: 376 KNATVRLLVSYWTHFVEGTDPYLKSLLYS-NILCNSSKFNDCFGKVEIKYYVVPGYNETG 435
           +N  VR+L+S+W +    T P +   L+S N + N          ++++++VVP ++ T 
Sbjct: 369 RNVQVRMLISHWNY----TSPIIPQWLHSLNQVDN----------IQVRWFVVPDFS-TN 428

Query: 436 PALYHNGTRTKNEYPGFSRVQHGKFAVSDVRAHIGTSNLGWDYFYVTSGVSFGTYNPAIV 495
           P +             ++RV H KF V++ ++++GTSN   DYF VT G+S+  +N    
Sbjct: 429 PQI------------PYTRVNHAKFMVTEKQSYVGTSNWSQDYFTVTGGLSYNVFNNDFT 436

Query: 496 KQLQEVFDADWNSPYTLPVK 511
            QLQ +F+ DWNSPYT P++
Sbjct: 489 NQLQSIFNRDWNSPYTQPIQ 436

BLAST of Cucsa.271500.1 vs. Swiss-Prot
Match: PLD3_PONAB (Phospholipase D3 OS=Pongo abelii GN=PLD3 PE=2 SV=1)

HSP 1 Score: 141.4 bits (355), Expect = 2.9e-32
Identity = 133/525 (25.33%), Postives = 221/525 (42.10%), Query Frame = 1

Query: 2   KKAQTPTLTTI-----FSALFFFFFVSQFPPVLSFTQSQ--------CKGWLVQSIPTDM 61
           KKA+   L  I     F AL    F+ ++  +  F  +Q        C+  LV+SIP  +
Sbjct: 34  KKARWVLLVLILAVVGFGALMTQLFLWEYGDLHLFGPNQRPAPCYDPCEAVLVESIPEGL 93

Query: 62  PQLQLVQGVLSTRDVFVWLAGNSTRRLDIIAQYWELLAGPNDSRSGDYGYSDEDLKKFGA 121
                  G  ST   ++ L   +   LDI + YW L              +D   ++  A
Sbjct: 94  DFPNASTGNPSTSQAWLGLLAGAHSSLDIASFYWTLTN------------NDTHTQEPSA 153

Query: 122 NKGYDVYSAIEKAADRNVNVRLVSHSGVSPNYGKEPADLASGRPNVQNVTLLLGDWYGSG 181
            +G +V   ++  A + VNVR+       PN  +  ADL +   +   V ++       G
Sbjct: 154 QQGEEVLRQLQTLAPKGVNVRIAVSK---PNGPQPQADLQALLQSGAQVRMVDMQKLTHG 213

Query: 182 IVHSKVWISDDRDVYIGSANQDWKSLSQVKELGIYLTGCPKIAAHVKVYFENLLKLAFLN 241
           ++H+K W+ D    Y+GSAN DW+SL+QVKELG+ +  C  +A                 
Sbjct: 214 VLHTKFWVVDQTHFYLGSANMDWRSLTQVKELGVVMYNCSCLA----------------- 273

Query: 242 RTDYTRTIFDHQW---QTQRKVP-CWSYFIDPESRCRSPLPPYVKFLHTLGYPTISDPYT 301
             D T+ IF+  W   Q    +P  W  F D      +P+   +      G P ++    
Sbjct: 274 -RDLTK-IFEAYWFLGQAGSSIPSTWPRFYDTRYNQETPMEICLN-----GTPALA---- 333

Query: 302 LNLSIQTPGSRLSTLLPHSCYLSFAPPELLFGRYQSDEQAWSDTIKSVSSGATVRISTMD 361
                               YL+ APP L       D +A  + + +  S   + ++ M+
Sbjct: 334 --------------------YLASAPPPLCPSGRTPDLKALLNVVDNARS--FIYVAVMN 393

Query: 362 WLGQSEFTTPTIYWSSLSSAISEVVFSKNATVRLLVSYWTHFVEGTDPYLKSLLYSNILC 421
           +L   EF+ P  +W ++   +    + +   VRLL+S W H    ++P +++ L S    
Sbjct: 394 YLPTLEFSHPHRFWPAIDDGLRRAAYERGVKVRLLISCWGH----SEPSMRAFLLS---L 453

Query: 422 NSSKFNDCFGKVEIKYYVVPGYNETGPALYHNGTRTKNEYPGFSRVQHGKFAVSDVRAHI 481
            + + N     +++K +VVP                +   P ++RV H K+ V++   +I
Sbjct: 454 AALRDNHTHSDIQVKLFVVP------------ADEAQARIP-YARVNHNKYMVTERATYI 473

Query: 482 GTSNLGWDYFYVTSGVS-FGTYN--PAIVKQLQEVFDADWNSPYT 507
           GTSN   +YF  T+G S   T N    +  QL+ +F  DW+SPY+
Sbjct: 514 GTSNWSGNYFTETAGTSLLVTQNGRGGLRSQLEAIFLRDWDSPYS 473

BLAST of Cucsa.271500.1 vs. Swiss-Prot
Match: PLD3_HUMAN (Phospholipase D3 OS=Homo sapiens GN=PLD3 PE=1 SV=1)

HSP 1 Score: 141.4 bits (355), Expect = 2.9e-32
Identity = 135/526 (25.67%), Postives = 223/526 (42.40%), Query Frame = 1

Query: 2   KKAQTPTLTTI-----FSALFFFFFVSQFPPVLSFTQSQ--------CKGWLVQSIPTDM 61
           KKA+   L  I     F AL    F+ ++  +  F  +Q        C+  LV+SIP  +
Sbjct: 34  KKARWVLLVLILAVVGFGALMTQLFLWEYGDLHLFGPNQRPAPCYDPCEAVLVESIPEGL 93

Query: 62  PQLQLVQGVLSTRDVFVWLAGNSTRRLDIIAQYWELLAGPNDSRSGDYGYSDEDLKKFGA 121
                  G  ST   ++ L   +   LDI + YW L              +D   ++  A
Sbjct: 94  DFPNASTGNPSTSQAWLGLLAGAHSSLDIASFYWTLTN------------NDTHTQEPSA 153

Query: 122 NKGYDVYSAIEKAADRNVNVRLVSHSGVSPNYGKEP-ADLASGRPNVQNVTLLLGDWYGS 181
            +G +V   ++  A + VNVR+     VS   G +P ADL +   +   V ++       
Sbjct: 154 QQGEEVLRQLQTLAPKGVNVRIA----VSKPSGPQPQADLQALLQSGAQVRMVDMQKLTH 213

Query: 182 GIVHSKVWISDDRDVYIGSANQDWKSLSQVKELGIYLTGCPKIAAHVKVYFENLLKLAFL 241
           G++H+K W+ D    Y+GSAN DW+SL+QVKELG+ +  C  +A                
Sbjct: 214 GVLHTKFWVVDQTHFYLGSANMDWRSLTQVKELGVVMYNCSCLA---------------- 273

Query: 242 NRTDYTRTIFDHQW---QTQRKVP-CWSYFIDPESRCRSPLPPYVKFLHTLGYPTISDPY 301
              D T+ IF+  W   Q    +P  W  F D      +P+   +      G P ++   
Sbjct: 274 --RDLTK-IFEAYWFLGQAGSSIPSTWPRFYDTRYNQETPMEICLN-----GTPALA--- 333

Query: 302 TLNLSIQTPGSRLSTLLPHSCYLSFAPPELLFGRYQSDEQAWSDTIKSVSSGATVRISTM 361
                                YL+ APP L       D +A  + + +  S   + ++ M
Sbjct: 334 ---------------------YLASAPPPLCPSGRTPDLKALLNVVDNARS--FIYVAVM 393

Query: 362 DWLGQSEFTTPTIYWSSLSSAISEVVFSKNATVRLLVSYWTHFVEGTDPYLKSLLYSNIL 421
           ++L   EF+ P  +W ++   +    + +   VRLL+S W H    ++P +++ L S   
Sbjct: 394 NYLPTLEFSHPHRFWPAIDDGLRRATYERGVKVRLLISCWGH----SEPSMRAFLLS--- 453

Query: 422 CNSSKFNDCFGKVEIKYYVVPGYNETGPALYHNGTRTKNEYPGFSRVQHGKFAVSDVRAH 481
             + + N     +++K +VVP                +   P ++RV H K+ V++   +
Sbjct: 454 LAALRDNHTHSDIQVKLFVVP------------ADEAQARIP-YARVNHNKYMVTERATY 473

Query: 482 IGTSNLGWDYFYVTSGVS-FGTYN--PAIVKQLQEVFDADWNSPYT 507
           IGTSN   +YF  T+G S   T N    +  QL+ +F  DW+SPY+
Sbjct: 514 IGTSNWSGNYFTETAGTSLLVTQNGRGGLRSQLEAIFLRDWDSPYS 473

BLAST of Cucsa.271500.1 vs. Swiss-Prot
Match: PLD3_MACFA (Phospholipase D3 OS=Macaca fascicularis GN=PLD3 PE=2 SV=1)

HSP 1 Score: 141.4 bits (355), Expect = 2.9e-32
Identity = 134/533 (25.14%), Postives = 218/533 (40.90%), Query Frame = 1

Query: 2   KKAQTPTLTTI-----FSALFFFFFVSQFPPVLSFTQSQ--------CKGWLVQSIPTDM 61
           KKA+   L  I     F AL    F+ ++  +  F  +Q        C+  LV+SIP  +
Sbjct: 34  KKARWVLLVLILAVVGFGALMTQLFLWEYGDLHLFGPNQRPAPCYDPCEAVLVESIPEGL 93

Query: 62  PQLQLVQGVLSTRDVFVWLAGNSTRRLDIIAQYWELLAGPNDSRSGDYGYSDEDLKKFGA 121
                  G  ST   ++ L   +   LDI + YW L              +D   ++  A
Sbjct: 94  DFPNASTGNPSTSQAWLGLLAGAHSSLDIASFYWTLTN------------NDTHTQEPSA 153

Query: 122 NKGYDVYSAIEKAADRNVNVRLVSHSGVSPNYGKEPADLASGRPNVQNVTLLLGDWYGSG 181
            +G +V   ++  A + VNVR+       PN  +   DL +   +   V ++       G
Sbjct: 154 QQGEEVLRQLQTLAPKGVNVRIAVSK---PNGPQPQTDLQALLQSGAQVRMVDMQKLTHG 213

Query: 182 IVHSKVWISDDRDVYIGSANQDWKSLSQVKELGIYLTGCPKIAAHVKVYFENLLKLAFLN 241
           ++H+K W+ D    Y+GSAN DW+SL+QVKELG+ +  C  +A                 
Sbjct: 214 VLHTKFWVVDQTHFYLGSANMDWRSLTQVKELGVVMYNCSCLA----------------- 273

Query: 242 RTDYTRTIFDHQW---QTQRKVP-CWSYFIDPESRCRSPLPPYVKFLHTLGYPTISDPYT 301
             D T+ IF+  W   Q    +P  W  F D      +P+   +      G P ++    
Sbjct: 274 -RDLTK-IFEAYWFLGQAGSSIPSTWPRFYDTRYNQETPMEICLN-----GTPALA---- 333

Query: 302 LNLSIQTPGSRLSTLLPHSCYLSFAPPELLFGRYQSDEQAWSDTIKSVSSGATVRISTMD 361
                               YL+ APP L       D +A  + + +  S   + I+ M+
Sbjct: 334 --------------------YLASAPPPLCPSGRTPDLKALLNVVDNARS--FIYIAVMN 393

Query: 362 WLGQSEFTTPTIYWSSLSSAISEVVFSKNATVRLLVSYWTHFVEGTDPYLKSLLYSNILC 421
           +L   EF+ P  +W ++   +    + +   VRLLVS W H       +L SL       
Sbjct: 394 YLPTLEFSHPHRFWPAIDDGLRRAAYERGVKVRLLVSCWRHSESSMRAFLLSLA------ 453

Query: 422 NSSKFNDCFGKVEIKYYVVPGYNETGPALYHNGTRTKNEYPGFSRVQHGKFAVSDVRAHI 481
            + + N     +++K +VVP                +   P ++RV H K+ V++   +I
Sbjct: 454 -ALRDNHTHSDIQVKLFVVP------------ADEAQARIP-YARVNHNKYMVTERATYI 481

Query: 482 GTSNLGWDYFYVTSGVS---FGTYNPAIVKQLQEVFDADWNSPYTLPVKAIQD 515
           GTSN   +YF  T+G S         ++  QL+ +F  DW+SPY+  + A  D
Sbjct: 514 GTSNWSGNYFTETAGTSLLVMQNGRGSLRSQLEAIFLRDWDSPYSHDLDASAD 481

BLAST of Cucsa.271500.1 vs. Swiss-Prot
Match: K4_VACCW (Phospholipase-D-like protein K4 OS=Vaccinia virus (strain Western Reserve) GN=VACWR035 PE=3 SV=1)

HSP 1 Score: 127.5 bits (319), Expect = 4.3e-28
Identity = 128/476 (26.89%), Postives = 200/476 (42.02%), Query Frame = 1

Query: 40  LVQSIPTDMPQLQLVQGVLSTRDVFVWLAGNSTRRLDIIAQYWELLAGPNDSRSGDYGYS 99
           + ++IP  M   Q  +  LST +++  +  N+T+ LDI + YW L              S
Sbjct: 10  ITETIPIGM---QFDKVYLSTFNMWREILSNTTKTLDISSFYWSL--------------S 69

Query: 100 DEDLKKFGANKGYDVYSAIEKAADRNVNVRLVSHSGVSPNYGKEPADLASGRPNVQNVTL 159
           DE     G N G  + + I +   R V VR+  +    P    E   +A       ++T 
Sbjct: 70  DE----VGTNFGTIILNKIVQLPKRGVRVRVAVNKSNKPLKDVERLQMAGVEVRYIDITN 129

Query: 160 LLGDWYGSGIVHSKVWISDDRDVYIGSANQDWKSLSQVKELGIYLTGCPKIAAHVKVYFE 219
           +LG     G++H+K WISD+  +Y+GSAN DW+SL+QVKELGI                 
Sbjct: 130 ILG-----GVLHTKFWISDNTHIYLGSANMDWRSLTQVKELGI----------------- 189

Query: 220 NLLKLAFLNRT---DYTRTIFDHQWQTQ-RKVPC-WSYFIDPESRCRSPLPPYVKFLHTL 279
                 F NR    D T+ IF+  W      +P  W  F           P Y    H L
Sbjct: 190 ----AIFNNRNLAADLTQ-IFEVYWYLGVNNLPYNWKNFY----------PSYYNTDHPL 249

Query: 280 GYPTISDPYTLNLSIQTPGSRLSTLLPHSCYLSFAPPELLFGRYQSDEQAWSDTIKSVSS 339
                        SI   G      +PHS +++ AP +L      +D  A    I++ S 
Sbjct: 250 -------------SINVSG------VPHSVFIASAPQQLCTMERTNDLTALLSCIRNASK 309

Query: 340 GATVRISTMDWLGQSEFTTPTI-YWSSLSSAISEVVFSKNATVRLLVSYWTHFVEGTDPY 399
              V +S M+++         I +W  +   +      +  +V+LL+S W    + +   
Sbjct: 310 --FVYVSVMNFIPIIYSKAGNILFWPYIEDELRRAAIDRQVSVKLLISCW----QRSSFI 369

Query: 400 LKSLLYSNILCNSSKFNDCFGKVEIKYYVVPGYNETGPALYHNGTRTKNEYPGFSRVQHG 459
           +++ L S  +  S   N     +E+K ++VP  +   P               +SRV H 
Sbjct: 370 MRNFLRSIAMLKSKNIN-----IEVKLFIVPDADPPIP---------------YSRVNHA 382

Query: 460 KFAVSDVRAHIGTSNLGWDYFYVTSGVSFGTY---NPAIVKQLQEVFDADWNSPYT 507
           K+ V+D  A+IGTSN   +YF  T G S          + +QL+++F  DWNS Y+
Sbjct: 430 KYMVTDKTAYIGTSNWTGNYFTDTCGASINITPDDGLGLRQQLEDIFMRDWNSKYS 382

BLAST of Cucsa.271500.1 vs. TrEMBL
Match: A0A0A0L498_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G651930 PE=4 SV=1)

HSP 1 Score: 1076.6 bits (2783), Expect = 0.0e+00
Identity = 520/520 (100.00%), Postives = 520/520 (100.00%), Query Frame = 1

Query: 1   MKKAQTPTLTTIFSALFFFFFVSQFPPVLSFTQSQCKGWLVQSIPTDMPQLQLVQGVLST 60
           MKKAQTPTLTTIFSALFFFFFVSQFPPVLSFTQSQCKGWLVQSIPTDMPQLQLVQGVLST
Sbjct: 1   MKKAQTPTLTTIFSALFFFFFVSQFPPVLSFTQSQCKGWLVQSIPTDMPQLQLVQGVLST 60

Query: 61  RDVFVWLAGNSTRRLDIIAQYWELLAGPNDSRSGDYGYSDEDLKKFGANKGYDVYSAIEK 120
           RDVFVWLAGNSTRRLDIIAQYWELLAGPNDSRSGDYGYSDEDLKKFGANKGYDVYSAIEK
Sbjct: 61  RDVFVWLAGNSTRRLDIIAQYWELLAGPNDSRSGDYGYSDEDLKKFGANKGYDVYSAIEK 120

Query: 121 AADRNVNVRLVSHSGVSPNYGKEPADLASGRPNVQNVTLLLGDWYGSGIVHSKVWISDDR 180
           AADRNVNVRLVSHSGVSPNYGKEPADLASGRPNVQNVTLLLGDWYGSGIVHSKVWISDDR
Sbjct: 121 AADRNVNVRLVSHSGVSPNYGKEPADLASGRPNVQNVTLLLGDWYGSGIVHSKVWISDDR 180

Query: 181 DVYIGSANQDWKSLSQVKELGIYLTGCPKIAAHVKVYFENLLKLAFLNRTDYTRTIFDHQ 240
           DVYIGSANQDWKSLSQVKELGIYLTGCPKIAAHVKVYFENLLKLAFLNRTDYTRTIFDHQ
Sbjct: 181 DVYIGSANQDWKSLSQVKELGIYLTGCPKIAAHVKVYFENLLKLAFLNRTDYTRTIFDHQ 240

Query: 241 WQTQRKVPCWSYFIDPESRCRSPLPPYVKFLHTLGYPTISDPYTLNLSIQTPGSRLSTLL 300
           WQTQRKVPCWSYFIDPESRCRSPLPPYVKFLHTLGYPTISDPYTLNLSIQTPGSRLSTLL
Sbjct: 241 WQTQRKVPCWSYFIDPESRCRSPLPPYVKFLHTLGYPTISDPYTLNLSIQTPGSRLSTLL 300

Query: 301 PHSCYLSFAPPELLFGRYQSDEQAWSDTIKSVSSGATVRISTMDWLGQSEFTTPTIYWSS 360
           PHSCYLSFAPPELLFGRYQSDEQAWSDTIKSVSSGATVRISTMDWLGQSEFTTPTIYWSS
Sbjct: 301 PHSCYLSFAPPELLFGRYQSDEQAWSDTIKSVSSGATVRISTMDWLGQSEFTTPTIYWSS 360

Query: 361 LSSAISEVVFSKNATVRLLVSYWTHFVEGTDPYLKSLLYSNILCNSSKFNDCFGKVEIKY 420
           LSSAISEVVFSKNATVRLLVSYWTHFVEGTDPYLKSLLYSNILCNSSKFNDCFGKVEIKY
Sbjct: 361 LSSAISEVVFSKNATVRLLVSYWTHFVEGTDPYLKSLLYSNILCNSSKFNDCFGKVEIKY 420

Query: 421 YVVPGYNETGPALYHNGTRTKNEYPGFSRVQHGKFAVSDVRAHIGTSNLGWDYFYVTSGV 480
           YVVPGYNETGPALYHNGTRTKNEYPGFSRVQHGKFAVSDVRAHIGTSNLGWDYFYVTSGV
Sbjct: 421 YVVPGYNETGPALYHNGTRTKNEYPGFSRVQHGKFAVSDVRAHIGTSNLGWDYFYVTSGV 480

Query: 481 SFGTYNPAIVKQLQEVFDADWNSPYTLPVKAIQDGPTFSS 521
           SFGTYNPAIVKQLQEVFDADWNSPYTLPVKAIQDGPTFSS
Sbjct: 481 SFGTYNPAIVKQLQEVFDADWNSPYTLPVKAIQDGPTFSS 520

BLAST of Cucsa.271500.1 vs. TrEMBL
Match: A0A067KBJ9_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_07083 PE=4 SV=1)

HSP 1 Score: 729.6 bits (1882), Expect = 2.8e-207
Identity = 345/501 (68.86%), Postives = 409/501 (81.64%), Query Frame = 1

Query: 20  FFVSQFPPVLSFTQSQCKGWLVQSIPTDMPQLQLVQGVLSTRDVFVWLAGNSTRRLDIIA 79
           FF++Q         S+CK WLVQSIPTDMPQL+ V GVL+T DV  WLAGNST+ LDIIA
Sbjct: 20  FFITQ-------ADSKCKAWLVQSIPTDMPQLRRVPGVLTTGDVLRWLAGNSTKGLDIIA 79

Query: 80  QYWELLAGPNDSRSGDYGYSDEDLKKFGANKGYDVYSAIEKAADRNVNVRLVSHSGVSPN 139
           QYW+L+A   D RSGDYGYS  D++KFGA +G+ VY AIE AADRNV++RL+ HSGV P+
Sbjct: 80  QYWQLIANSKDPRSGDYGYSKSDMEKFGAFEGFGVYKAIENAADRNVSIRLLQHSGVYPD 139

Query: 140 YGKEPADLASGRPNVQNVTLLLGDWYGSGIVHSKVWISDDRDVYIGSANQDWKSLSQVKE 199
           + KEP DLASGR NV NVTLLLG+W+GSGIVH+KVWISD RDVYIGSAN DWKSL+QVKE
Sbjct: 140 FTKEPNDLASGRLNVDNVTLLLGEWWGSGIVHAKVWISDSRDVYIGSANNDWKSLTQVKE 199

Query: 200 LGIYLTGCPKIAAHVKVYFENLLKLAFLNRTDYTRTIFDHQWQTQRKVPCWSYFIDPESR 259
           +GIYL GC KI   V+ YFENL KLA LN + YTR+++D QWQ  R VPCWS+FID ++R
Sbjct: 200 VGIYLVGCKKIVGKVETYFENLWKLAHLNSSAYTRSVWDQQWQINRTVPCWSHFIDSKAR 259

Query: 260 CRSPLPPYVKFLHTLGYPTISDPYTLNLSIQTPGSRLSTLLPHSCYLSFAPPELLFGRYQ 319
           CRSPLP +V+  H  GYP +SDP+ + + I+TPG R STL P+  YLSFAPPEL FG+YQ
Sbjct: 260 CRSPLPTFVEVPHVAGYPVLSDPHMIKVPIKTPGQRFSTLEPYLSYLSFAPPELSFGKYQ 319

Query: 320 SDEQAWSDTIKSVSSGATVRISTMDWLGQSEFTTPTIYWSSLSSAISEVVFSKNATVRLL 379
           +DEQ W DTIKSV SG+TVRI+TMDWLGQS+FT   +YWSSLSSAISEV+FSK+ATV++L
Sbjct: 320 TDEQGWIDTIKSVGSGSTVRINTMDWLGQSQFTKEKVYWSSLSSAISEVIFSKHATVKVL 379

Query: 380 VSYWTHFVEGTDPYLKSLLYSNILCNSSKFNDCFGKVEIKYYVVPGYNETGPALYHNGTR 439
           V+YW HF++ TD YLKSLLYS+ILCNSSK+N C GKVEIKYYVVPGYN TGPA   NG R
Sbjct: 380 VAYWAHFIDNTDQYLKSLLYSDILCNSSKYNKCHGKVEIKYYVVPGYNLTGPAT-RNGKR 439

Query: 440 TKNEYPGFSRVQHGKFAVSDVRAHIGTSNLGWDYFYVTSGVSFGTYNPAIVKQLQEVFDA 499
           T N+YP F+RV HGK+AVSDVRAHIGTSNL WDYFY T+GVSFGTYNPAIV QLQ++FDA
Sbjct: 440 TGNKYPAFTRVNHGKYAVSDVRAHIGTSNLVWDYFYTTAGVSFGTYNPAIVLQLQKIFDA 499

Query: 500 DWNSPYTLPVKAIQDGPTFSS 521
           DW+SPY +P + ++ G  FSS
Sbjct: 500 DWDSPYAVPFEGLEAGHAFSS 512

BLAST of Cucsa.271500.1 vs. TrEMBL
Match: V4UMB2_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10008002mg PE=4 SV=1)

HSP 1 Score: 729.6 bits (1882), Expect = 2.8e-207
Identity = 343/517 (66.34%), Postives = 412/517 (79.69%), Query Frame = 1

Query: 7   PTLTTIFSALFFFFFVSQFPPVL---SFTQSQCKGWLVQSIPTDMPQLQLVQGVLSTRDV 66
           P   T  S + F  F    P +    S ++++CK WLVQSIPTDMP L  V G++S+ +V
Sbjct: 3   PRYRTSLSLVIFILFTLYLPDITTSSSSSRTKCKAWLVQSIPTDMPHLPRVPGIVSSGNV 62

Query: 67  FVWLAGNSTRRLDIIAQYWELLAGPNDSRSGDYGYSDEDLKKFGANKGYDVYSAIEKAAD 126
             WLAGNST+RLDIIAQYW+L+A PN+SRSGDYGYS++D+K+FGA +G+DVY +IE AAD
Sbjct: 63  LKWLAGNSTKRLDIIAQYWQLIARPNNSRSGDYGYSEDDMKRFGAQEGFDVYKSIENAAD 122

Query: 127 RNVNVRLVSHSGVSPNYGKEPADLASGRPNVQNVTLLLGDWYGSGIVHSKVWISDDRDVY 186
           RNV VRL+ H GV P++  E ++LASGR NV+NVTLLLGDW+GSGIVH+KVWISD RDVY
Sbjct: 123 RNVRVRLLQHLGVYPDFTTEASNLASGRKNVENVTLLLGDWWGSGIVHAKVWISDCRDVY 182

Query: 187 IGSANQDWKSLSQVKELGIYLTGCPKIAAHVKVYFENLLKLAFLNRTDYTRTIFDHQWQT 246
           IGSAN DWKSL+QVKE+GIYL GCP IA  V+VYFENL  L+ LN + YTRT+ D QWQ 
Sbjct: 183 IGSANNDWKSLTQVKEVGIYLVGCPTIAKKVEVYFENLWTLSSLNSSTYTRTVSDQQWQA 242

Query: 247 QRKVPCWSYFIDPESRCRSPLPPYVKFLHTLGYPTISDPYTLNLSIQTPGSRLSTLLPHS 306
            R VPCWS+F+DP+ RCRSPLPP ++  H  GYP +SDPY    +++TPGS  STL PH 
Sbjct: 243 NRTVPCWSHFLDPKGRCRSPLPPVLEVPHVEGYPILSDPYMFRETMETPGSNYSTLQPHY 302

Query: 307 CYLSFAPPELLFGRYQSDEQAWSDTIKSVSSGATVRISTMDWLGQSEFTTPTIYWSSLSS 366
            YLSFAPPEL FG+Y +DEQAW DTIKSV + AT+RISTMDWLGQS++   T+YWS+LSS
Sbjct: 303 SYLSFAPPELSFGKYMADEQAWVDTIKSVGTRATLRISTMDWLGQSQYLKQTVYWSALSS 362

Query: 367 AISEVVFSKNATVRLLVSYWTHFVEGTDPYLKSLLYSNILCNSSKFNDCFGKVEIKYYVV 426
           A+SEVVFSK+A V++LV+YW HF+  TD YLKSLLYSN+LCNSSK+N C GKVEIKYY V
Sbjct: 363 AVSEVVFSKHANVKILVAYWAHFINNTDEYLKSLLYSNVLCNSSKYNKCSGKVEIKYYKV 422

Query: 427 PGYNETGPALYHNGTRTKNEYPGFSRVQHGKFAVSDVRAHIGTSNLGWDYFYVTSGVSFG 486
           PGYN TGPA+  NG+ T N YPGF+RV HGK+AVSD RAHIGTSNL WDYFY T+GVSFG
Sbjct: 423 PGYNLTGPAI-QNGSNTGNIYPGFTRVNHGKYAVSDSRAHIGTSNLVWDYFYTTAGVSFG 482

Query: 487 TYNPAIVKQLQEVFDADWNSPYTLPVKAIQDGPTFSS 521
           TYNPAIV QLQE+FDADWNSPY  PV+ + DG   +S
Sbjct: 483 TYNPAIVSQLQEIFDADWNSPYAFPVEELGDGHAHAS 518

BLAST of Cucsa.271500.1 vs. TrEMBL
Match: A0A067FW62_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g009992mg PE=4 SV=1)

HSP 1 Score: 729.6 bits (1882), Expect = 2.8e-207
Identity = 343/517 (66.34%), Postives = 412/517 (79.69%), Query Frame = 1

Query: 7   PTLTTIFSALFFFFFVSQFPPVL---SFTQSQCKGWLVQSIPTDMPQLQLVQGVLSTRDV 66
           P   T  S + F  F    P +    S ++++CK WLVQSIPTDMP L  V G++S+ +V
Sbjct: 3   PRYRTSLSLVIFILFTLYLPDITTSSSSSRTKCKAWLVQSIPTDMPHLPRVPGIVSSGNV 62

Query: 67  FVWLAGNSTRRLDIIAQYWELLAGPNDSRSGDYGYSDEDLKKFGANKGYDVYSAIEKAAD 126
             WLAGNST+RLDIIAQYW+L+A PN+SRSGDYGYS++D+K+FGA +G+DVY +IE AAD
Sbjct: 63  LKWLAGNSTKRLDIIAQYWQLIARPNNSRSGDYGYSEDDMKRFGAQEGFDVYKSIENAAD 122

Query: 127 RNVNVRLVSHSGVSPNYGKEPADLASGRPNVQNVTLLLGDWYGSGIVHSKVWISDDRDVY 186
           RNV VRL+ H GV P++  E ++LASGR NV+NVTLLLGDW+GSGIVH+KVWISD RDVY
Sbjct: 123 RNVRVRLLQHLGVYPDFTTEASNLASGRKNVENVTLLLGDWWGSGIVHAKVWISDCRDVY 182

Query: 187 IGSANQDWKSLSQVKELGIYLTGCPKIAAHVKVYFENLLKLAFLNRTDYTRTIFDHQWQT 246
           IGSAN DWKSL+QVKE+GIYL GCP IA  V+VYFENL  L+ LN + YTRT+ D QWQ 
Sbjct: 183 IGSANNDWKSLTQVKEVGIYLVGCPTIAKKVEVYFENLWTLSSLNSSTYTRTVSDQQWQA 242

Query: 247 QRKVPCWSYFIDPESRCRSPLPPYVKFLHTLGYPTISDPYTLNLSIQTPGSRLSTLLPHS 306
            R VPCWS+F+DP+ RCRSPLPP ++  H  GYP +SDPY    +++TPGS  STL PH 
Sbjct: 243 NRTVPCWSHFLDPKGRCRSPLPPVLEVPHVEGYPILSDPYMFRETMETPGSNYSTLQPHY 302

Query: 307 CYLSFAPPELLFGRYQSDEQAWSDTIKSVSSGATVRISTMDWLGQSEFTTPTIYWSSLSS 366
            YLSFAPPEL FG+Y +DEQAW DTIKSV + AT+RISTMDWLGQS++   T+YWS+LSS
Sbjct: 303 SYLSFAPPELSFGKYMADEQAWVDTIKSVGTRATLRISTMDWLGQSQYLKQTVYWSALSS 362

Query: 367 AISEVVFSKNATVRLLVSYWTHFVEGTDPYLKSLLYSNILCNSSKFNDCFGKVEIKYYVV 426
           A+SEVVFSK+A V++LV+YW HF+  TD YLKSLLYSN+LCNSSK+N C GKVEIKYY V
Sbjct: 363 AVSEVVFSKHANVKILVAYWAHFINNTDEYLKSLLYSNVLCNSSKYNKCSGKVEIKYYKV 422

Query: 427 PGYNETGPALYHNGTRTKNEYPGFSRVQHGKFAVSDVRAHIGTSNLGWDYFYVTSGVSFG 486
           PGYN TGPA+  NG+ T N YPGF+RV HGK+AVSD RAHIGTSNL WDYFY T+GVSFG
Sbjct: 423 PGYNLTGPAI-QNGSNTGNIYPGFTRVNHGKYAVSDSRAHIGTSNLVWDYFYTTAGVSFG 482

Query: 487 TYNPAIVKQLQEVFDADWNSPYTLPVKAIQDGPTFSS 521
           TYNPAIV QLQE+FDADWNSPY  PV+ + DG   +S
Sbjct: 483 TYNPAIVSQLQEIFDADWNSPYAFPVEELGDGHAHAS 518

BLAST of Cucsa.271500.1 vs. TrEMBL
Match: D7SV42_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_04s0008g06620 PE=4 SV=1)

HSP 1 Score: 724.2 bits (1868), Expect = 1.2e-205
Identity = 343/510 (67.25%), Postives = 407/510 (79.80%), Query Frame = 1

Query: 11  TIFSALFFFFFVSQFPPVLSFTQSQCKGWLVQSIPTDMPQLQLVQGVLSTRDVFVWLAGN 70
           T   ++F    V Q   V + + SQCK W+VQSIPT MP L  V GVLST DVF WLAGN
Sbjct: 5   TTLKSVFIVLLVIQALGVNAKSSSQCKAWIVQSIPTHMPHLPPVSGVLSTADVFQWLAGN 64

Query: 71  STRRLDIIAQYWELLAGPNDSRSGDYGYSDEDLKKFGANKGYDVYSAIEKAADRNVNVRL 130
           S+  LDIIAQYW+L A P+D  SGDYGYS  D+ KFGA++G+DVY A+E AADRN+++RL
Sbjct: 65  SSLSLDIIAQYWQLNAHPDDPLSGDYGYSKADMHKFGAHEGFDVYRALENAADRNISIRL 124

Query: 131 VSHSGVSPNYGKEPADLASGRPNVQNVTLLLGDWYGSGIVHSKVWISDDRDVYIGSANQD 190
           + HSGV P+Y KEP+DLASGRPNV+NVTLLLG+W+GSGIVH+KVWISD RDVYIGSAN D
Sbjct: 125 LQHSGVYPDYTKEPSDLASGRPNVENVTLLLGEWWGSGIVHAKVWISDSRDVYIGSANND 184

Query: 191 WKSLSQVKELGIYLTGCPKIAAHVKVYFENLLKLAFLNRTDYTRTIFDHQWQTQRKVPCW 250
           WKSL+QVKE+GIYL  CP IA  V++Y+ NL KLA LN + YT T++D QWQ  R+VPCW
Sbjct: 185 WKSLTQVKEVGIYLVDCPSIARQVEIYYNNLWKLASLNVSAYTTTVWDQQWQINRQVPCW 244

Query: 251 SYFIDPESRCRSPLPPYVKFLHTLGYPTISDPYTLNLSIQTPGSRLSTLLPHSCYLSFAP 310
           S+F+ P  RCRSPLP YV   H +GYPT+SDP      IQTPG   ST  P S  LSFAP
Sbjct: 245 SHFLTPRERCRSPLPRYVAVPHVVGYPTLSDPRMFKTPIQTPGHNCSTSKPQSSCLSFAP 304

Query: 311 PELLFGRYQSDEQAWSDTIKSVSSGATVRISTMDWLGQSEFTTPTIYWSSLSSAISEVVF 370
           PEL FG++Q+DEQAW DTIKSV +GATVRI++MDWLGQS++   T+YWSSLSSA+SEVVF
Sbjct: 305 PELSFGKHQADEQAWVDTIKSVGTGATVRINSMDWLGQSQYMQQTVYWSSLSSAVSEVVF 364

Query: 371 SKNATVRLLVSYWTHFVEGTDPYLKSLLYSNILCNSSKFNDCFGKVEIKYYVVPGYNETG 430
           SK+A V++LV+YW HF+  TD YLKSLLYSN+LC SSK+N C GKVEIKYY VPG+N TG
Sbjct: 365 SKHAKVKILVAYWAHFINNTDQYLKSLLYSNVLCYSSKYNKCSGKVEIKYYKVPGFNLTG 424

Query: 431 PALYHNGTRTKNEYPGFSRVQHGKFAVSDVRAHIGTSNLGWDYFYVTSGVSFGTYNPAIV 490
           PA+ HNGT T N YPGF+RV HGK+AVSDVRAHIGTSNL WDYFY T+GVSFGTYNPAIV
Sbjct: 425 PAI-HNGTSTGNIYPGFTRVNHGKYAVSDVRAHIGTSNLVWDYFYSTAGVSFGTYNPAIV 484

Query: 491 KQLQEVFDADWNSPYTLPVKAIQDGPTFSS 521
            QLQE+FDADWNSPY +PV+ +++G   SS
Sbjct: 485 SQLQEIFDADWNSPYAVPVEPLEEGQFHSS 513

BLAST of Cucsa.271500.1 vs. NCBI nr
Match: gi|778696916|ref|XP_011654227.1| (PREDICTED: phospholipase D Y-like [Cucumis sativus])

HSP 1 Score: 1076.6 bits (2783), Expect = 0.0e+00
Identity = 520/520 (100.00%), Postives = 520/520 (100.00%), Query Frame = 1

Query: 1   MKKAQTPTLTTIFSALFFFFFVSQFPPVLSFTQSQCKGWLVQSIPTDMPQLQLVQGVLST 60
           MKKAQTPTLTTIFSALFFFFFVSQFPPVLSFTQSQCKGWLVQSIPTDMPQLQLVQGVLST
Sbjct: 1   MKKAQTPTLTTIFSALFFFFFVSQFPPVLSFTQSQCKGWLVQSIPTDMPQLQLVQGVLST 60

Query: 61  RDVFVWLAGNSTRRLDIIAQYWELLAGPNDSRSGDYGYSDEDLKKFGANKGYDVYSAIEK 120
           RDVFVWLAGNSTRRLDIIAQYWELLAGPNDSRSGDYGYSDEDLKKFGANKGYDVYSAIEK
Sbjct: 61  RDVFVWLAGNSTRRLDIIAQYWELLAGPNDSRSGDYGYSDEDLKKFGANKGYDVYSAIEK 120

Query: 121 AADRNVNVRLVSHSGVSPNYGKEPADLASGRPNVQNVTLLLGDWYGSGIVHSKVWISDDR 180
           AADRNVNVRLVSHSGVSPNYGKEPADLASGRPNVQNVTLLLGDWYGSGIVHSKVWISDDR
Sbjct: 121 AADRNVNVRLVSHSGVSPNYGKEPADLASGRPNVQNVTLLLGDWYGSGIVHSKVWISDDR 180

Query: 181 DVYIGSANQDWKSLSQVKELGIYLTGCPKIAAHVKVYFENLLKLAFLNRTDYTRTIFDHQ 240
           DVYIGSANQDWKSLSQVKELGIYLTGCPKIAAHVKVYFENLLKLAFLNRTDYTRTIFDHQ
Sbjct: 181 DVYIGSANQDWKSLSQVKELGIYLTGCPKIAAHVKVYFENLLKLAFLNRTDYTRTIFDHQ 240

Query: 241 WQTQRKVPCWSYFIDPESRCRSPLPPYVKFLHTLGYPTISDPYTLNLSIQTPGSRLSTLL 300
           WQTQRKVPCWSYFIDPESRCRSPLPPYVKFLHTLGYPTISDPYTLNLSIQTPGSRLSTLL
Sbjct: 241 WQTQRKVPCWSYFIDPESRCRSPLPPYVKFLHTLGYPTISDPYTLNLSIQTPGSRLSTLL 300

Query: 301 PHSCYLSFAPPELLFGRYQSDEQAWSDTIKSVSSGATVRISTMDWLGQSEFTTPTIYWSS 360
           PHSCYLSFAPPELLFGRYQSDEQAWSDTIKSVSSGATVRISTMDWLGQSEFTTPTIYWSS
Sbjct: 301 PHSCYLSFAPPELLFGRYQSDEQAWSDTIKSVSSGATVRISTMDWLGQSEFTTPTIYWSS 360

Query: 361 LSSAISEVVFSKNATVRLLVSYWTHFVEGTDPYLKSLLYSNILCNSSKFNDCFGKVEIKY 420
           LSSAISEVVFSKNATVRLLVSYWTHFVEGTDPYLKSLLYSNILCNSSKFNDCFGKVEIKY
Sbjct: 361 LSSAISEVVFSKNATVRLLVSYWTHFVEGTDPYLKSLLYSNILCNSSKFNDCFGKVEIKY 420

Query: 421 YVVPGYNETGPALYHNGTRTKNEYPGFSRVQHGKFAVSDVRAHIGTSNLGWDYFYVTSGV 480
           YVVPGYNETGPALYHNGTRTKNEYPGFSRVQHGKFAVSDVRAHIGTSNLGWDYFYVTSGV
Sbjct: 421 YVVPGYNETGPALYHNGTRTKNEYPGFSRVQHGKFAVSDVRAHIGTSNLGWDYFYVTSGV 480

Query: 481 SFGTYNPAIVKQLQEVFDADWNSPYTLPVKAIQDGPTFSS 521
           SFGTYNPAIVKQLQEVFDADWNSPYTLPVKAIQDGPTFSS
Sbjct: 481 SFGTYNPAIVKQLQEVFDADWNSPYTLPVKAIQDGPTFSS 520

BLAST of Cucsa.271500.1 vs. NCBI nr
Match: gi|659104211|ref|XP_008452848.1| (PREDICTED: phospholipase D Y-like [Cucumis melo])

HSP 1 Score: 1031.6 bits (2666), Expect = 4.9e-298
Identity = 498/521 (95.59%), Postives = 509/521 (97.70%), Query Frame = 1

Query: 1   MKKAQTPTLTTIFSAL-FFFFFVSQFPPVLSFTQSQCKGWLVQSIPTDMPQLQLVQGVLS 60
           MKK  + T+TTIFSAL FFFFF+SQFPPVLSFT+SQCKGWLVQSIPTDMPQLQLVQGVLS
Sbjct: 1   MKKTHSLTVTTIFSALLFFFFFISQFPPVLSFTRSQCKGWLVQSIPTDMPQLQLVQGVLS 60

Query: 61  TRDVFVWLAGNSTRRLDIIAQYWELLAGPNDSRSGDYGYSDEDLKKFGANKGYDVYSAIE 120
           TRDVFVWLAGNSTRRLDIIAQYWELLAGPNDSRSGDYGYS+EDLKKFGANKGYDVYSAIE
Sbjct: 61  TRDVFVWLAGNSTRRLDIIAQYWELLAGPNDSRSGDYGYSNEDLKKFGANKGYDVYSAIE 120

Query: 121 KAADRNVNVRLVSHSGVSPNYGKEPADLASGRPNVQNVTLLLGDWYGSGIVHSKVWISDD 180
           KAADRNVNVRLVSHSGV P +GKEPADLASGRPNVQNVTLLLGDWYGSGIVHSKVWISDD
Sbjct: 121 KAADRNVNVRLVSHSGVYPTFGKEPADLASGRPNVQNVTLLLGDWYGSGIVHSKVWISDD 180

Query: 181 RDVYIGSANQDWKSLSQVKELGIYLTGCPKIAAHVKVYFENLLKLAFLNRTDYTRTIFDH 240
           RDVYIGSANQDWKSLSQVKELGIYLTGCPKIAAHVKVYF+NLLKLAFLN TDYTRTIFDH
Sbjct: 181 RDVYIGSANQDWKSLSQVKELGIYLTGCPKIAAHVKVYFDNLLKLAFLNPTDYTRTIFDH 240

Query: 241 QWQTQRKVPCWSYFIDPESRCRSPLPPYVKFLHTLGYPTISDPYTLNLSIQTPGSRLSTL 300
           QWQTQRKVPCWSYFIDPESRCRSPLPPYVKFLHTLGYP ISDPYTLNLSIQTPGSRLSTL
Sbjct: 241 QWQTQRKVPCWSYFIDPESRCRSPLPPYVKFLHTLGYPIISDPYTLNLSIQTPGSRLSTL 300

Query: 301 LPHSCYLSFAPPELLFGRYQSDEQAWSDTIKSVSSGATVRISTMDWLGQSEFTTPTIYWS 360
           LPHSCYLSFAPPELLFGRYQSDEQAWSDTIKSVS+GATVRISTMDWLGQSEFT PTIYWS
Sbjct: 301 LPHSCYLSFAPPELLFGRYQSDEQAWSDTIKSVSNGATVRISTMDWLGQSEFTNPTIYWS 360

Query: 361 SLSSAISEVVFSKNATVRLLVSYWTHFVEGTDPYLKSLLYSNILCNSSKFNDCFGKVEIK 420
           SLSSAISEVVFSKNATVRLLVSYWTHFVEGTDPYLKSLLYSNILCNSSKFNDCFGKVEIK
Sbjct: 361 SLSSAISEVVFSKNATVRLLVSYWTHFVEGTDPYLKSLLYSNILCNSSKFNDCFGKVEIK 420

Query: 421 YYVVPGYNETGPALYHNGTRTKNEYPGFSRVQHGKFAVSDVRAHIGTSNLGWDYFYVTSG 480
           YYVVPGYNETGPALYHN TRT+N YP F+RVQHGK+AVSDVRAHIGTSNLGWDYFYVTSG
Sbjct: 421 YYVVPGYNETGPALYHNRTRTENRYPSFTRVQHGKYAVSDVRAHIGTSNLGWDYFYVTSG 480

Query: 481 VSFGTYNPAIVKQLQEVFDADWNSPYTLPVKAIQDGPTFSS 521
           VSFGTYNPAIVKQLQEVFDADWNSPYTLPVKAIQDGPTFSS
Sbjct: 481 VSFGTYNPAIVKQLQEVFDADWNSPYTLPVKAIQDGPTFSS 521

BLAST of Cucsa.271500.1 vs. NCBI nr
Match: gi|747070305|ref|XP_011081968.1| (PREDICTED: phospholipase D Z-like [Sesamum indicum])

HSP 1 Score: 741.9 bits (1914), Expect = 7.7e-211
Identity = 351/521 (67.37%), Postives = 424/521 (81.38%), Query Frame = 1

Query: 1   MKKAQTPTLTTIFSALFFF-FFVSQFPPVLSFTQSQCKGWLVQSIPTDMPQLQLVQGVLS 60
           MK   TP L T++  L +    V       ++   QCK WLVQSIPTDMP L LV GVLS
Sbjct: 1   MKTCATPLLLTLYFLLIYNPTRVVHSSSDGNYDDGQCKAWLVQSIPTDMPHLSLVPGVLS 60

Query: 61  TRDVFVWLAGNSTRRLDIIAQYWELLAGPNDSRSGDYGYSDEDLKKFGANKGYDVYSAIE 120
           T DV  WLAGNS+R LDIIAQYW+L+A P D RSGDYGY+++D+++FGAN+G++V+ A+E
Sbjct: 61  TADVLRWLAGNSSRSLDIIAQYWQLVAHPKDPRSGDYGYTEDDMRRFGANQGFEVHQALE 120

Query: 121 KAADRNVNVRLVSHSGVSPNYGKEPADLASGRPNVQNVTLLLGDWYGSGIVHSKVWISDD 180
            AADRN+++RL+ HSGV P+Y +EP+ LASGRPNV+NVTLLL DW+GSGIVH+KVWISD 
Sbjct: 121 NAADRNISIRLLQHSGVYPDYTEEPSALASGRPNVKNVTLLLKDWWGSGIVHAKVWISDS 180

Query: 181 RDVYIGSANQDWKSLSQVKELGIYLTGCPKIAAHVKVYFENLLKLAFLNRTDYTRTIFDH 240
           +DVYIGSAN DWKSL+QVKE+GIYL GCP+IA +V++YFENL KLA LN ++YT +I+D 
Sbjct: 181 QDVYIGSANNDWKSLTQVKEVGIYLVGCPRIAENVEIYFENLWKLAHLNSSNYTESIWDQ 240

Query: 241 QWQTQRKVPCWSYFIDPESRCRSPLPPYVKFLHTLGYPTISDPYTLNLSIQTPGSRLSTL 300
           QWQ  RKVPCWS+F+ P+ RCRSPLP YVK  H  GYP +SDP   ++SI+TPG   ST 
Sbjct: 241 QWQIVRKVPCWSHFLHPKERCRSPLPKYVKVKHVTGYPVLSDPDIFHVSIETPGRNYSTS 300

Query: 301 LPHSCYLSFAPPELLFGRYQSDEQAWSDTIKSVSSGATVRISTMDWLGQSEFTTPTIYWS 360
              SCYLSFAPPELLFG+YQ+DEQAW DTIKSV +GAT+RISTMDWLGQS++T+ T+YWS
Sbjct: 301 QHQSCYLSFAPPELLFGKYQTDEQAWVDTIKSVGNGATLRISTMDWLGQSQYTSQTVYWS 360

Query: 361 SLSSAISEVVFSKNATVRLLVSYWTHFVEGTDPYLKSLLYSNILCNSSKFNDCFGKVEIK 420
           SLSSAISEVVFSK A V+LLV+YW HF+  TD YLKSLLY+N LC+SSK+N C GKVEIK
Sbjct: 361 SLSSAISEVVFSKQAKVKLLVAYWAHFINNTDVYLKSLLYTNNLCSSSKYNKCHGKVEIK 420

Query: 421 YYVVPGYNETGPALYHNGTRTKNEYPGFSRVQHGKFAVSDVRAHIGTSNLGWDYFYVTSG 480
           YY+VPG+N TGPA   NGT T N YPG++RV HGK+AVSDVRAHIGTSNL WDYFY T+G
Sbjct: 421 YYMVPGFNLTGPAT-QNGTATGNIYPGYTRVNHGKYAVSDVRAHIGTSNLIWDYFYSTAG 480

Query: 481 VSFGTYNPAIVKQLQEVFDADWNSPYTLPVKAIQDGPTFSS 521
           VSFGTYN AIV QLQE+FDADW+SPY +PV+ ++DG  FSS
Sbjct: 481 VSFGTYNSAIVSQLQEIFDADWDSPYAVPVEPLEDGHAFSS 520

BLAST of Cucsa.271500.1 vs. NCBI nr
Match: gi|643724311|gb|KDP33512.1| (hypothetical protein JCGZ_07083 [Jatropha curcas])

HSP 1 Score: 729.6 bits (1882), Expect = 4.0e-207
Identity = 345/501 (68.86%), Postives = 409/501 (81.64%), Query Frame = 1

Query: 20  FFVSQFPPVLSFTQSQCKGWLVQSIPTDMPQLQLVQGVLSTRDVFVWLAGNSTRRLDIIA 79
           FF++Q         S+CK WLVQSIPTDMPQL+ V GVL+T DV  WLAGNST+ LDIIA
Sbjct: 20  FFITQ-------ADSKCKAWLVQSIPTDMPQLRRVPGVLTTGDVLRWLAGNSTKGLDIIA 79

Query: 80  QYWELLAGPNDSRSGDYGYSDEDLKKFGANKGYDVYSAIEKAADRNVNVRLVSHSGVSPN 139
           QYW+L+A   D RSGDYGYS  D++KFGA +G+ VY AIE AADRNV++RL+ HSGV P+
Sbjct: 80  QYWQLIANSKDPRSGDYGYSKSDMEKFGAFEGFGVYKAIENAADRNVSIRLLQHSGVYPD 139

Query: 140 YGKEPADLASGRPNVQNVTLLLGDWYGSGIVHSKVWISDDRDVYIGSANQDWKSLSQVKE 199
           + KEP DLASGR NV NVTLLLG+W+GSGIVH+KVWISD RDVYIGSAN DWKSL+QVKE
Sbjct: 140 FTKEPNDLASGRLNVDNVTLLLGEWWGSGIVHAKVWISDSRDVYIGSANNDWKSLTQVKE 199

Query: 200 LGIYLTGCPKIAAHVKVYFENLLKLAFLNRTDYTRTIFDHQWQTQRKVPCWSYFIDPESR 259
           +GIYL GC KI   V+ YFENL KLA LN + YTR+++D QWQ  R VPCWS+FID ++R
Sbjct: 200 VGIYLVGCKKIVGKVETYFENLWKLAHLNSSAYTRSVWDQQWQINRTVPCWSHFIDSKAR 259

Query: 260 CRSPLPPYVKFLHTLGYPTISDPYTLNLSIQTPGSRLSTLLPHSCYLSFAPPELLFGRYQ 319
           CRSPLP +V+  H  GYP +SDP+ + + I+TPG R STL P+  YLSFAPPEL FG+YQ
Sbjct: 260 CRSPLPTFVEVPHVAGYPVLSDPHMIKVPIKTPGQRFSTLEPYLSYLSFAPPELSFGKYQ 319

Query: 320 SDEQAWSDTIKSVSSGATVRISTMDWLGQSEFTTPTIYWSSLSSAISEVVFSKNATVRLL 379
           +DEQ W DTIKSV SG+TVRI+TMDWLGQS+FT   +YWSSLSSAISEV+FSK+ATV++L
Sbjct: 320 TDEQGWIDTIKSVGSGSTVRINTMDWLGQSQFTKEKVYWSSLSSAISEVIFSKHATVKVL 379

Query: 380 VSYWTHFVEGTDPYLKSLLYSNILCNSSKFNDCFGKVEIKYYVVPGYNETGPALYHNGTR 439
           V+YW HF++ TD YLKSLLYS+ILCNSSK+N C GKVEIKYYVVPGYN TGPA   NG R
Sbjct: 380 VAYWAHFIDNTDQYLKSLLYSDILCNSSKYNKCHGKVEIKYYVVPGYNLTGPAT-RNGKR 439

Query: 440 TKNEYPGFSRVQHGKFAVSDVRAHIGTSNLGWDYFYVTSGVSFGTYNPAIVKQLQEVFDA 499
           T N+YP F+RV HGK+AVSDVRAHIGTSNL WDYFY T+GVSFGTYNPAIV QLQ++FDA
Sbjct: 440 TGNKYPAFTRVNHGKYAVSDVRAHIGTSNLVWDYFYTTAGVSFGTYNPAIVLQLQKIFDA 499

Query: 500 DWNSPYTLPVKAIQDGPTFSS 521
           DW+SPY +P + ++ G  FSS
Sbjct: 500 DWDSPYAVPFEGLEAGHAFSS 512

BLAST of Cucsa.271500.1 vs. NCBI nr
Match: gi|802626330|ref|XP_012076426.1| (PREDICTED: phospholipase D Z-like [Jatropha curcas])

HSP 1 Score: 729.6 bits (1882), Expect = 4.0e-207
Identity = 345/501 (68.86%), Postives = 409/501 (81.64%), Query Frame = 1

Query: 20  FFVSQFPPVLSFTQSQCKGWLVQSIPTDMPQLQLVQGVLSTRDVFVWLAGNSTRRLDIIA 79
           FF++Q         S+CK WLVQSIPTDMPQL+ V GVL+T DV  WLAGNST+ LDIIA
Sbjct: 23  FFITQ-------ADSKCKAWLVQSIPTDMPQLRRVPGVLTTGDVLRWLAGNSTKGLDIIA 82

Query: 80  QYWELLAGPNDSRSGDYGYSDEDLKKFGANKGYDVYSAIEKAADRNVNVRLVSHSGVSPN 139
           QYW+L+A   D RSGDYGYS  D++KFGA +G+ VY AIE AADRNV++RL+ HSGV P+
Sbjct: 83  QYWQLIANSKDPRSGDYGYSKSDMEKFGAFEGFGVYKAIENAADRNVSIRLLQHSGVYPD 142

Query: 140 YGKEPADLASGRPNVQNVTLLLGDWYGSGIVHSKVWISDDRDVYIGSANQDWKSLSQVKE 199
           + KEP DLASGR NV NVTLLLG+W+GSGIVH+KVWISD RDVYIGSAN DWKSL+QVKE
Sbjct: 143 FTKEPNDLASGRLNVDNVTLLLGEWWGSGIVHAKVWISDSRDVYIGSANNDWKSLTQVKE 202

Query: 200 LGIYLTGCPKIAAHVKVYFENLLKLAFLNRTDYTRTIFDHQWQTQRKVPCWSYFIDPESR 259
           +GIYL GC KI   V+ YFENL KLA LN + YTR+++D QWQ  R VPCWS+FID ++R
Sbjct: 203 VGIYLVGCKKIVGKVETYFENLWKLAHLNSSAYTRSVWDQQWQINRTVPCWSHFIDSKAR 262

Query: 260 CRSPLPPYVKFLHTLGYPTISDPYTLNLSIQTPGSRLSTLLPHSCYLSFAPPELLFGRYQ 319
           CRSPLP +V+  H  GYP +SDP+ + + I+TPG R STL P+  YLSFAPPEL FG+YQ
Sbjct: 263 CRSPLPTFVEVPHVAGYPVLSDPHMIKVPIKTPGQRFSTLEPYLSYLSFAPPELSFGKYQ 322

Query: 320 SDEQAWSDTIKSVSSGATVRISTMDWLGQSEFTTPTIYWSSLSSAISEVVFSKNATVRLL 379
           +DEQ W DTIKSV SG+TVRI+TMDWLGQS+FT   +YWSSLSSAISEV+FSK+ATV++L
Sbjct: 323 TDEQGWIDTIKSVGSGSTVRINTMDWLGQSQFTKEKVYWSSLSSAISEVIFSKHATVKVL 382

Query: 380 VSYWTHFVEGTDPYLKSLLYSNILCNSSKFNDCFGKVEIKYYVVPGYNETGPALYHNGTR 439
           V+YW HF++ TD YLKSLLYS+ILCNSSK+N C GKVEIKYYVVPGYN TGPA   NG R
Sbjct: 383 VAYWAHFIDNTDQYLKSLLYSDILCNSSKYNKCHGKVEIKYYVVPGYNLTGPAT-RNGKR 442

Query: 440 TKNEYPGFSRVQHGKFAVSDVRAHIGTSNLGWDYFYVTSGVSFGTYNPAIVKQLQEVFDA 499
           T N+YP F+RV HGK+AVSDVRAHIGTSNL WDYFY T+GVSFGTYNPAIV QLQ++FDA
Sbjct: 443 TGNKYPAFTRVNHGKYAVSDVRAHIGTSNLVWDYFYTTAGVSFGTYNPAIVLQLQKIFDA 502

Query: 500 DWNSPYTLPVKAIQDGPTFSS 521
           DW+SPY +P + ++ G  FSS
Sbjct: 503 DWDSPYAVPFEGLEAGHAFSS 515

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
PLDY_DICDI1.6e-4628.00Phospholipase D Y OS=Dictyostelium discoideum GN=pldY PE=2 SV=1[more]
PLD3_PONAB2.9e-3225.33Phospholipase D3 OS=Pongo abelii GN=PLD3 PE=2 SV=1[more]
PLD3_HUMAN2.9e-3225.67Phospholipase D3 OS=Homo sapiens GN=PLD3 PE=1 SV=1[more]
PLD3_MACFA2.9e-3225.14Phospholipase D3 OS=Macaca fascicularis GN=PLD3 PE=2 SV=1[more]
K4_VACCW4.3e-2826.89Phospholipase-D-like protein K4 OS=Vaccinia virus (strain Western Reserve) GN=VA... [more]
Match NameE-valueIdentityDescription
A0A0A0L498_CUCSA0.0e+00100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_4G651930 PE=4 SV=1[more]
A0A067KBJ9_JATCU2.8e-20768.86Uncharacterized protein OS=Jatropha curcas GN=JCGZ_07083 PE=4 SV=1[more]
V4UMB2_9ROSI2.8e-20766.34Uncharacterized protein OS=Citrus clementina GN=CICLE_v10008002mg PE=4 SV=1[more]
A0A067FW62_CITSI2.8e-20766.34Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g009992mg PE=4 SV=1[more]
D7SV42_VITVI1.2e-20567.25Putative uncharacterized protein OS=Vitis vinifera GN=VIT_04s0008g06620 PE=4 SV=... [more]
Match NameE-valueIdentityDescription
gi|778696916|ref|XP_011654227.1|0.0e+00100.00PREDICTED: phospholipase D Y-like [Cucumis sativus][more]
gi|659104211|ref|XP_008452848.1|4.9e-29895.59PREDICTED: phospholipase D Y-like [Cucumis melo][more]
gi|747070305|ref|XP_011081968.1|7.7e-21167.37PREDICTED: phospholipase D Z-like [Sesamum indicum][more]
gi|643724311|gb|KDP33512.1|4.0e-20768.86hypothetical protein JCGZ_07083 [Jatropha curcas][more]
gi|802626330|ref|XP_012076426.1|4.0e-20768.86PREDICTED: phospholipase D Z-like [Jatropha curcas][more]
The following terms have been associated with this mRNA:
Vocabulary: INTERPRO
TermDefinition
IPR001736PLipase_D/transphosphatidylase
IPR025202PLD-like_dom
Vocabulary: Molecular Function
TermDefinition
GO:0003824catalytic activity
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008152 metabolic process
cellular_component GO:0005575 cellular_component
molecular_function GO:0003824 catalytic activity

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Cucsa.271500Cucsa.271500gene


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Cucsa.271500.1Cucsa.271500.1-proteinpolypeptide


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsa.271500.1.CDS.1Cucsa.271500.1.CDS.1CDS
Cucsa.271500.1.CDS.2Cucsa.271500.1.CDS.2CDS
Cucsa.271500.1.CDS.3Cucsa.271500.1.CDS.3CDS
Cucsa.271500.1.CDS.4Cucsa.271500.1.CDS.4CDS
Cucsa.271500.1.CDS.5Cucsa.271500.1.CDS.5CDS
Cucsa.271500.1.CDS.6Cucsa.271500.1.CDS.6CDS
Cucsa.271500.1.CDS.7Cucsa.271500.1.CDS.7CDS


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsa.271500.1.three_prime_UTR.1Cucsa.271500.1.three_prime_UTR.1three_prime_UTR


Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001736Phospholipase D/TransphosphatidylaseSMARTSM00155pld_4coord: 166..193
score: 2.7E-5coord: 447..473
score: 0.
IPR001736Phospholipase D/TransphosphatidylasePROFILEPS50035PLDcoord: 166..193
score: 12.511coord: 447..473
score:
IPR025202Phospholipase D-like domainPFAMPF13091PLDc_2coord: 111..221
score: 2.6
NoneNo IPR availableGENE3DG3DSA:3.30.870.10coord: 337..409
score: 9.5E-14coord: 448..508
score: 9.5E-14coord: 52..223
score: 5.1
NoneNo IPR availablePANTHERPTHR10185PHOSPHOLIPASE D - RELATEDcoord: 246..270
score: 3.5E-155coord: 300..515
score: 3.5E-155coord: 33..229
score: 3.5E
NoneNo IPR availablePANTHERPTHR10185:SF17PHOSPHOLIPASE D F09G2.8-RELATEDcoord: 246..270
score: 3.5E-155coord: 300..515
score: 3.5E-155coord: 33..229
score: 3.5E
NoneNo IPR availableunknownSSF56024Phospholipase D/nucleasecoord: 111..224
score: 1.91E-18coord: 58..83
score: 1.91E-18coord: 307..513
score: 1.7