Cucsa.109540 (gene) Cucumber (Gy14) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cucsa.109540
Type	gene
Organism	Cucumis sativus (Cucumber (Gy14) v1)
Description	Isoflavone reductase family protein
Location	scaffold00934 : 464260 .. 466594 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TCCCACCCACCGGTCAAAGCAAATATTTTTTAACAACGACAAAGGCACTCCACGGTACTCCTCCGATCAACTATTTCTCCGCCGGGAACTCCTTACTCCGATGGCTCAGAAGGTTCTTATCATCGGAGGAACTGGCTACATCGGTAAATTCATCGTACAAGCCAGCGCCAAAGCTGGTCATCCTACATATGCATTGATCCGCAGGTCTTCGCTCGAAAGCCCCGCCAAGAATCGAATCCTCAATCATTTCAAAAGCTTAGGAGTCAATTTTCTCTTCGTAAGTACATTTCTCTGGATTTTTTTTGTTTCATTGCTCATAATTATTTTGTAATCCTGATTTTCTAGGTCTGATTTGATTTTAGTCGTGGTTACTTATAATGTAGCGTACTGTTGATTCTTTTGAGTTATTCACATTATTAGTTCTTGAATTCTGTGGAACCATAATGAATTTGATTGGATATGGTATTGTTTAATTTGGCTGAAACTATGAAAATTGGGACAGGGTGATCTCTTTGACAATGAGAGTTTGGTTAAGGCGATTAAGCAGGTTGATGTGGTGATATCAACCCTTGGTGGCCATATGGTTCCCCATCAACATAAAATCCTATCTGCTATCAAGCAAGCTGGCAATGTGAAGGTAATCTTATTCTACATTTTCCTTACAACTATGTCATTGATTGTGATTGTTTTGCTGCAATATCTATTTTTCTAGTAGAGTTTGTGCGTTTTGGGAATTTTGATCATTGGAATTTGTGTGCCACAGAGGTTCTTTCCTTCTGAGTTCGGTAACGATGCGGATCATATCGATGCAGTGGAACCAGCGAAATCTATGTATGCTGCAAAGGCTGAATTTCGTAGGGCTATTGAAGCTGAGGGAATCCCACATACATTTGTGGTATGCAATTTCTTCGATGGTTACTTTCTTAGCAACTTGTCTCAGCCAGATGCAAGCGTCCCACCTAGAGATAAAGTCGTTATCTTAGGAGATGGAACTCCCAAAGGTACAATATGTCATTAAATTTATCTTCCCCATCAGCTCAAGTTTTTAAGTAAAATGGTAAATTGACATTGTTATCAGAATCTAGAAAAAAAATCATGTGTTTAAACTTCTCCAGTGTTATTTCCTTCCTAATTAATATTGATTTCACTTGTTGGACTTTGTACATATTTCAATCTCACAAGAGAGAGTTAGATGATATAACATTAAATCTACCTTCATCCATGAGTGTAAGCTTTTAGTCAATTGCCGATTCGAAATTATCTTTATTCTCATGAGTTCTAATTGAATCTTTTTTTATTGTGGGATAAATTGTGGTACATACCATATCCTATTTTCTTGAGTTGAATGGTTTCCAAACCTTCCTTGGAAATCAAATAGATAATGATGCATTTCATTTGGCTTTTTATTCATCAAACCAATTGCTGTATATTATTGTCTTCAATATGGTGTTTCAATAGCCTTCCACGCTATTCATTCTGTATCGGATTTGTTTGTCAAAATAGCGATTTGCATCAAGGAAGAAGCTCTTCTAATGAACAAAACTTCTGCATATATACACACGTGTTATAAATAATTGATTGCAACATGAACCATCATTCATACATCGTACTTAGTTTCTTGAAGGGAGATATAGAATTTGTTTCAACAGCCATTCACCCTATTCATTATGTCTTTGTTATGTTTCATGAAGTCATTTACAACAAGGAAGAAGATGTTGGAACGTACACAATCCGAGCAATAGATGATCCAAGAACCCTCAACAAGATCATGTACCTTAGGCCCCCAGCTAACATCTACTCAACCAATGATCTCGTGTCGCTGTGGGAGAGAAAAATTGGCAAGAGTCTTAAAAGAATTTATGTACCAGAGGAAGAAGTATTGAAGAAAATCCGAGGTGGGTTTTTTAAGCAAAGCAACAAACTTTCAATTCTTATATTCTATTGTATGCTTCTAATCACAAACTGACTAACAACACAATTGCTTTCGCTAATCATTTCGTTTTCCAGAGACTTCATATCCACTTAATATAGAATTGGCACTATGCCACACTGCTCAAGTGAAGGGATGTCAAACTAATTTTAGTATTGAACCATCTTTTGGAGTTGAAGCTTCAGCATTGTACCCAGATGTTCGGTATACAACTGTAGAAGAATACCTCGACCAGTTTGTCTAAAGAAAAAGTCTTTTGTTTTTGTTTTTTTTTTTTTTTTACTTTTGTCATTTTGGCATAATGGGTGTAAGTCTTAGGCCGTTGGGTGTTATGTATTCATGTATAATAAGGAAATGCTCTTATTTGAAAATAGAGGTTCTTACATAATTCAAAGTTTAGAATGA

mRNA sequence

TCCCACCCACCGGTCAAAGCAAATATTTTTTAACAACGACAAAGGCACTCCACGGTACTCCTCCGATCAACTATTTCTCCGCCGGGAACTCCTTACTCCGATGGCTCAGAAGGTTCTTATCATCGGAGGAACTGGCTACATCGGTAAATTCATCGTACAAGCCAGCGCCAAAGCTGGTCATCCTACATATGCATTGATCCGCAGGTCTTCGCTCGAAAGCCCCGCCAAGAATCGAATCCTCAATCATTTCAAAAGCTTAGGAGTCAATTTTCTCTTCGGTGATCTCTTTGACAATGAGAGTTTGGTTAAGGCGATTAAGCAGGTTGATGTGGTGATATCAACCCTTGGTGGCCATATGGTTCCCCATCAACATAAAATCCTATCTGCTATCAAGCAAGCTGGCAATGTGAAGAGGTTCTTTCCTTCTGAGTTCGGTAACGATGCGGATCATATCGATGCAGTGGAACCAGCGAAATCTATGTATGCTGCAAAGGCTGAATTTCGTAGGGCTATTGAAGCTGAGGGAATCCCACATACATTTGTGGTATGCAATTTCTTCGATGGTTACTTTCTTAGCAACTTGTCTCAGCCAGATGCAAGCGTCCCACCTAGAGATAAAGTCGTTATCTTAGGAGATGGAACTCCCAAAGTCATTTACAACAAGGAAGAAGATGTTGGAACGTACACAATCCGAGCAATAGATGATCCAAGAACCCTCAACAAGATCATGTACCTTAGGCCCCCAGCTAACATCTACTCAACCAATGATCTCGTGTCGCTGTGGGAGAGAAAAATTGGCAAGAGTCTTAAAAGAATTTATGTACCAGAGGAAGAAGTATTGAAGAAAATCCGAGAGACTTCATATCCACTTAATATAGAATTGGCACTATGCCACACTGCTCAAGTGAAGGGATGTCAAACTAATTTTAGTATTGAACCATCTTTTGGAGTTGAAGCTTCAGCATTGTACCCAGATGTTCGGTATACAACTGTAGAAGAATACCTCGACCAGTTTGTCTAAAGAAAAAGTCttttgtttttgttttttttttttttttacttttgtCATTTTGGCATAATGGGTGTAAGTCTTAGGCCGTTGGGTGTTATGTATTCATGTATAATAAGGAAATGCTCTTATTTGAAAATAGAGGTTCTTACATAATTCAAAGTTTAGAATGA

Coding sequence (CDS)

ATGGCTCAGAAGGTTCTTATCATCGGAGGAACTGGCTACATCGGTAAATTCATCGTACAAGCCAGCGCCAAAGCTGGTCATCCTACATATGCATTGATCCGCAGGTCTTCGCTCGAAAGCCCCGCCAAGAATCGAATCCTCAATCATTTCAAAAGCTTAGGAGTCAATTTTCTCTTCGGTGATCTCTTTGACAATGAGAGTTTGGTTAAGGCGATTAAGCAGGTTGATGTGGTGATATCAACCCTTGGTGGCCATATGGTTCCCCATCAACATAAAATCCTATCTGCTATCAAGCAAGCTGGCAATGTGAAGAGGTTCTTTCCTTCTGAGTTCGGTAACGATGCGGATCATATCGATGCAGTGGAACCAGCGAAATCTATGTATGCTGCAAAGGCTGAATTTCGTAGGGCTATTGAAGCTGAGGGAATCCCACATACATTTGTGGTATGCAATTTCTTCGATGGTTACTTTCTTAGCAACTTGTCTCAGCCAGATGCAAGCGTCCCACCTAGAGATAAAGTCGTTATCTTAGGAGATGGAACTCCCAAAGTCATTTACAACAAGGAAGAAGATGTTGGAACGTACACAATCCGAGCAATAGATGATCCAAGAACCCTCAACAAGATCATGTACCTTAGGCCCCCAGCTAACATCTACTCAACCAATGATCTCGTGTCGCTGTGGGAGAGAAAAATTGGCAAGAGTCTTAAAAGAATTTATGTACCAGAGGAAGAAGTATTGAAGAAAATCCGAGAGACTTCATATCCACTTAATATAGAATTGGCACTATGCCACACTGCTCAAGTGAAGGGATGTCAAACTAATTTTAGTATTGAACCATCTTTTGGAGTTGAAGCTTCAGCATTGTACCCAGATGTTCGGTATACAACTGTAGAAGAATACCTCGACCAGTTTGTCTAA

Protein sequence

MAQKVLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAKNRILNHFKSLGVNFLFGDLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHIDAVEPAKSMYAAKAEFRRAIEAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILGDGTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDLVSLWERKIGKSLKRIYVPEEEVLKKIRETSYPLNIELALCHTAQVKGCQTNFSIEPSFGVEASALYPDVRYTTVEEYLDQFV*

Homology

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BLAST of Cucsa.109540 vs. Swiss-Prot
Match: ALL12_OLEEU (Isoflavone reductase-like protein OS=Olea europaea PE=1 SV=1)

HSP 1 Score: 418.7 bits (1075), Expect = 5.5e-116
Identity = 201/303 (66.34%), Postives = 251/303 (82.84%), Query Frame = 1


Query: 4   KVLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAKNRILNHFKSLGVNFLFGDLF 63
           K+LIIGGTGYIGKFIV+ASAK+ HPT+AL R S++  P K +I+  FK+ GV  L GDL+
Sbjct: 6   KILIIGGTGYIGKFIVEASAKSEHPTFALARESTISDPVKGKIIQGFKNSGVTILTGDLY 65

Query: 64  DNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHIDAVEP 123
           D+ESLVKAIKQVDVVIST+G   +  Q KI++AIK+AGNVKRFFPS+FG D D   AVEP
Sbjct: 66  DHESLVKAIKQVDVVISTVGQLQLADQVKIIAAIKEAGNVKRFFPSDFGTDVDRCHAVEP 125

Query: 124 AKSMYAAKAEFRRAIEAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILGDGTPK 183
           AKS +  K++ RRAIEAEGIP+TFV  N+F GY L  L QP+ + PPRDKV+ILGDG  K
Sbjct: 126 AKSSFEIKSQIRRAIEAEGIPYTFVSANYFAGYSLPTLVQPEVTAPPRDKVIILGDGNAK 185

Query: 184 VIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDLVSLWERKIGKSLKRIYVPE 243
            ++N+E D+GTYTI+A+DD RTLNKI+Y++PP NIYS N+LV+LWE+KIGK+L++IYVPE
Sbjct: 186 AVFNEENDIGTYTIKAVDDARTLNKILYIKPPKNIYSFNELVALWEKKIGKTLEKIYVPE 245

Query: 244 EEVLKKIRETSYPLNIELALCHTAQVKGCQTNFSIEPSFGVEASALYPDVRYTTVEEYLD 303
           E+VLK+I+E+ +P+NI +A+ H+A VKG  TNF IEPSFGVEAS LYPDV+YTTVEEYLD
Sbjct: 246 EQVLKQIQESPFPINIVMAINHSAFVKGDLTNFKIEPSFGVEASELYPDVKYTTVEEYLD 305

Query: 304 QFV 307
           QFV
Sbjct: 306 QFV 308

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BLAST of Cucsa.109540 vs. Swiss-Prot
Match: IFRH_SOLTU (Isoflavone reductase homolog OS=Solanum tuberosum PE=2 SV=1)

HSP 1 Score: 410.6 bits (1054), Expect = 1.5e-113
Identity = 194/303 (64.03%), Postives = 248/303 (81.85%), Query Frame = 1


Query: 4   KVLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAKNRILNHFKSLGVNFLFGDLF 63
           K+L IGGTGYIGKFIV+ASAKAGH T+ L+R S+L +P K ++++ FKS GV F+ GDL+
Sbjct: 6   KILFIGGTGYIGKFIVEASAKAGHDTFVLVRESTLSNPTKTKLIDTFKSFGVTFVHGDLY 65

Query: 64  DNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHIDAVEP 123
           D+ESLVKAIKQVDVVIST+G  ++  Q K+++AIK+AGNVKRFFPSEFGND D + AVEP
Sbjct: 66  DHESLVKAIKQVDVVISTVGHALLADQVKLIAAIKEAGNVKRFFPSEFGNDVDRVHAVEP 125

Query: 124 AKSMYAAKAEFRRAIEAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILGDGTPK 183
           AK+ +  KA+ RR +EAEGIP T+V   FF GY L NL+QP A+ PP DKVVILG G  K
Sbjct: 126 AKAAFNTKAQIRRVVEAEGIPFTYVATFFFAGYSLPNLAQPGAAGPPNDKVVILGHGNTK 185

Query: 184 VIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDLVSLWERKIGKSLKRIYVPE 243
            ++NKEED+GTYTI A+DDP+TLNKI+Y++PP NI + N+LVSLWE+K GK+L+R+YVPE
Sbjct: 186 AVFNKEEDIGTYTINAVDDPKTLNKILYIKPPHNIITLNELVSLWEKKTGKNLERLYVPE 245

Query: 244 EEVLKKIRETSYPLNIELALCHTAQVKGCQTNFSIEPSFGVEASALYPDVRYTTVEEYLD 303
           E+VLK I+E S P+N+ L++ HTA VKG  TNF IEPSFGVEAS +YPDV+YT ++E L+
Sbjct: 246 EQVLKNIQEASVPMNVGLSIYHTAFVKGDHTNFEIEPSFGVEASEVYPDVKYTPIDEILN 305

Query: 304 QFV 307
           Q+V
Sbjct: 306 QYV 308

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BLAST of Cucsa.109540 vs. Swiss-Prot
Match: IFRH_TOBAC (Isoflavone reductase homolog A622 OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 387.9 bits (995), Expect = 1.0e-106
Identity = 181/303 (59.74%), Postives = 238/303 (78.55%), Query Frame = 1


Query: 4   KVLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAKNRILNHFKSLGVNFLFGDLF 63
           K+LIIGGTGYIGK++V+ SAK+GHPT+ALIR S+L++P K+++++ FKS GV  LFGD+ 
Sbjct: 8   KILIIGGTGYIGKYLVETSAKSGHPTFALIRESTLKNPEKSKLIDTFKSYGVTLLFGDIS 67

Query: 64  DNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHIDAVEP 123
           + ESL+KAIKQVDVVIST+GG     Q  I+ AIK+AGN+KRF PSEFG D DH  A+EP
Sbjct: 68  NQESLLKAIKQVDVVISTVGGQQFTDQVNIIKAIKEAGNIKRFLPSEFGFDVDHARAIEP 127

Query: 124 AKSMYAAKAEFRRAIEAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILGDGTPK 183
           A S++A K   RR IEAEGIP+T+V+CN+F  +FL NL Q +A  PPRDKVVI GDG PK
Sbjct: 128 AASLFALKVRIRRMIEAEGIPYTYVICNWFADFFLPNLGQLEAKTPPRDKVVIFGDGNPK 187

Query: 184 VIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDLVSLWERKIGKSLKRIYVPE 243
            IY KEED+ TYTI A+DDPRTLNK +++RPPANI S N++VSLWE KIGK+L+++Y+ E
Sbjct: 188 AIYVKEEDIATYTIEAVDDPRTLNKTLHMRPPANILSFNEIVSLWEDKIGKTLEKLYLSE 247

Query: 244 EEVLKKIRETSYPLNIELALCHTAQVKGCQTNFSIEPSFGVEASALYPDVRYTTVEEYLD 303
           E++L+ ++E   PL   LA+CH+  V G   NF ++P  GVEA+ LYP V+YTTV+E+ +
Sbjct: 248 EDILQIVQEGPLPLRTNLAICHSVFVNGDSANFEVQPPTGVEATELYPKVKYTTVDEFYN 307

Query: 304 QFV 307
           +FV
Sbjct: 308 KFV 310

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BLAST of Cucsa.109540 vs. Swiss-Prot
Match: IFRH_ARATH (Isoflavone reductase homolog P3 OS=Arabidopsis thaliana GN=At1g75280 PE=2 SV=1)

HSP 1 Score: 375.6 bits (963), Expect = 5.4e-103
Identity = 185/304 (60.86%), Postives = 232/304 (76.32%), Query Frame = 1


Query: 4   KVLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAKNRILNHFKSLGVNFLFGDLF 63
           K+L+IGGTGYIGKF+V+ASAKAGH T+AL+R ++L  P K + +  FK LGV  L GDL 
Sbjct: 7   KILVIGGTGYIGKFLVEASAKAGHSTFALVREATLSDPVKGKTVQSFKDLGVTILHGDLN 66

Query: 64  DNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHIDAVEP 123
           D+ESLVKAIKQVDVVIST+G   +  Q KI+SAIK+AGNVKRF PSEFG D D   AVEP
Sbjct: 67  DHESLVKAIKQVDVVISTVGSMQILDQTKIISAIKEAGNVKRFLPSEFGVDVDRTSAVEP 126

Query: 124 AKSMYAAKAEFRRAIEAEGIPHTFVVCNFFDGYFLSNLSQ--PDASVPPRDKVVILGDGT 183
           AKS +A K + RR IEAEGIP+T+ V   F GY+L  L Q  P  + PPRDKV ILGDG 
Sbjct: 127 AKSAFAGKIQIRRTIEAEGIPYTYAVTGCFGGYYLPTLVQFEPGLTSPPRDKVTILGDGN 186

Query: 184 PKVIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDLVSLWERKIGKSLKRIYV 243
            K + NKEED+  YTI+A+DDPRTLNKI+Y++P  N  S N++V+LWE+KIGKSL++ ++
Sbjct: 187 AKAVINKEEDIAAYTIKAVDDPRTLNKILYIKPSNNTLSMNEIVTLWEKKIGKSLEKTHL 246

Query: 244 PEEEVLKKIRETSYPLNIELALCHTAQVKGCQTNFSIEPSFGVEASALYPDVRYTTVEEY 303
           PEE++LK I+E+  P+N+ L++ H   V G  TN SIEPSFGVEAS LYPDV+YT+V+EY
Sbjct: 247 PEEQLLKSIQESPIPINVVLSINHAVFVNG-DTNISIEPSFGVEASELYPDVKYTSVDEY 306

Query: 304 LDQF 306
           L  F
Sbjct: 307 LSYF 309

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BLAST of Cucsa.109540 vs. Swiss-Prot
Match: IFRH_MAIZE (Isoflavone reductase homolog IRL OS=Zea mays GN=IRL PE=2 SV=1)

HSP 1 Score: 340.5 bits (872), Expect = 1.9e-92
Identity = 166/303 (54.79%), Postives = 218/303 (71.95%), Query Frame = 1


Query: 4   KVLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAKNRILNHFKSLGVNFLFGDLF 63
           K+L++GGTGY+G+ +V ASA+ GHPT AL+R ++   PAK  +L  F+  GV  L GDL+
Sbjct: 7   KILVVGGTGYLGRHVVAASARLGHPTSALVRDTAPSDPAKAALLKSFQDAGVTLLKGDLY 66

Query: 64  DNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHIDAVEP 123
           D  SLV A+K  DVVIS LG   +  Q +++ AIK+AGNVKRFFPSEFG D D    VEP
Sbjct: 67  DQASLVSAVKGADVVISVLGSMQIADQSRLVDAIKEAGNVKRFFPSEFGLDVDRTGIVEP 126

Query: 124 AKSMYAAKAEFRRAIEAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILGDGTPK 183
           AKS+  AK   RRA EA GIP+T+ V  FF G+ L  + Q  A  PP DK V+LGDG  K
Sbjct: 127 AKSILGAKVGIRRATEAAGIPYTYAVAGFFAGFGLPKVGQVLAPGPPADKAVVLGDGDTK 186

Query: 184 VIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDLVSLWERKIGKSLKRIYVPE 243
            ++ +E D+ TYT+ A DDPR  NK++Y++PPAN  S N+L+SLWE+K GK+ +R YVPE
Sbjct: 187 AVFVEEGDIATYTVLAADDPRAENKVLYIKPPANTLSHNELLSLWEKKTGKTFRREYVPE 246

Query: 244 EEVLKKIRETSYPLNIELALCHTAQVKGCQTNFSIEPSFGVEASALYPDVRYTTVEEYLD 303
           E VLK+I+E+  PLNI LA+ H A V+G QT F I+P+ GV+AS LYPDV+YTTV+EYL+
Sbjct: 247 EAVLKQIQESPIPLNIILAIGHAAFVRGEQTGFEIDPAKGVDASELYPDVKYTTVDEYLN 306

Query: 304 QFV 307
           +F+
Sbjct: 307 RFL 309

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BLAST of Cucsa.109540 vs. TrEMBL
Match: A0A0A0LJ01_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G248130 PE=4 SV=1)

HSP 1 Score: 613.2 bits (1580), Expect = 1.7e-172
Identity = 306/306 (100.00%), Postives = 306/306 (100.00%), Query Frame = 1


Query: 1   MAQKVLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAKNRILNHFKSLGVNFLFG 60
           MAQKVLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAKNRILNHFKSLGVNFLFG
Sbjct: 1   MAQKVLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAKNRILNHFKSLGVNFLFG 60

Query: 61  DLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHIDA 120
           DLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHIDA
Sbjct: 61  DLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHIDA 120

Query: 121 VEPAKSMYAAKAEFRRAIEAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILGDG 180
           VEPAKSMYAAKAEFRRAIEAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILGDG
Sbjct: 121 VEPAKSMYAAKAEFRRAIEAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILGDG 180

Query: 181 TPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDLVSLWERKIGKSLKRIY 240
           TPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDLVSLWERKIGKSLKRIY
Sbjct: 181 TPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDLVSLWERKIGKSLKRIY 240

Query: 241 VPEEEVLKKIRETSYPLNIELALCHTAQVKGCQTNFSIEPSFGVEASALYPDVRYTTVEE 300
           VPEEEVLKKIRETSYPLNIELALCHTAQVKGCQTNFSIEPSFGVEASALYPDVRYTTVEE
Sbjct: 241 VPEEEVLKKIRETSYPLNIELALCHTAQVKGCQTNFSIEPSFGVEASALYPDVRYTTVEE 300

Query: 301 YLDQFV 307
           YLDQFV
Sbjct: 301 YLDQFV 306

[Close]

BLAST of Cucsa.109540 vs. TrEMBL
Match: A0A0A0LL65_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G248135 PE=4 SV=1)

HSP 1 Score: 453.4 bits (1165), Expect = 2.2e-124
Identity = 220/308 (71.43%), Postives = 265/308 (86.04%), Query Frame = 1


Query: 1   MAQ--KVLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAKNRILNHFKSLGVNFL 60
           MAQ  KVL+IG TGYIGKF+V+ASA+AGHPT+AL+R+S+L   AK+ I+++F++LGVNF+
Sbjct: 1   MAQNLKVLVIGATGYIGKFVVEASAQAGHPTFALVRQSTLADSAKSSIIHNFRNLGVNFV 60

Query: 61  FGDLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHI 120
           FGD+FDNESLV+AI+QVDVVIST+G  ++ HQ KI+SAIKQAGNVKRF PSEFGND D +
Sbjct: 61  FGDIFDNESLVRAIQQVDVVISTVGRGLLSHQEKIISAIKQAGNVKRFLPSEFGNDVDRV 120

Query: 121 DAVEPAKSMYAAKAEFRRAIEAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILG 180
            AVEPAKSM+A+K E RRA+EAEGIPHTFVV NFFDGY+L N SQP A+ PPRDK+ I G
Sbjct: 121 HAVEPAKSMFASKVEIRRAVEAEGIPHTFVVSNFFDGYYLRNFSQPGATEPPRDKIKIFG 180

Query: 181 DGTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDLVSLWERKIGKSLKR 240
           DG  KVIYNKEED+GTYTIRAIDDPRT NKI+Y+RPPANIYSTN+LVSLWE+KIG+ L+R
Sbjct: 181 DGNLKVIYNKEEDIGTYTIRAIDDPRTFNKILYIRPPANIYSTNELVSLWEKKIGRILER 240

Query: 241 IYVPEEEVLKKIRETSYPLNIELALCHTAQVKGCQTNFSIEPSFGVEASALYPDVRYTTV 300
            YV EEE++K I+ET  PL+  LA+ H+A VKG  TNF IEPS GVEAS LYP+V YTTV
Sbjct: 241 TYVSEEELVKNIQETPVPLSTALAISHSAFVKGDHTNFEIEPSIGVEASELYPNVHYTTV 300

Query: 301 EEYLDQFV 307
           E+YL+QFV
Sbjct: 301 EDYLNQFV 308

[Close]

BLAST of Cucsa.109540 vs. TrEMBL
Match: M5VMM7_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa008220mg PE=4 SV=1)

HSP 1 Score: 448.4 bits (1152), Expect = 7.2e-123
Identity = 213/303 (70.30%), Postives = 258/303 (85.15%), Query Frame = 1


Query: 4   KVLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAKNRILNHFKSLGVNFLFGDLF 63
           K+L IGGTGYIGKFIV+ASAKAGHPTYAL+R  +L +PAK +++ +FKSLGVNF+ GDL+
Sbjct: 39  KILFIGGTGYIGKFIVEASAKAGHPTYALVREPTLSNPAKAKVIENFKSLGVNFVLGDLY 98

Query: 64  DNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHIDAVEP 123
           D+ESLVKAIKQVDVVIST+G   +  Q  I++AIK+AGNVKRFFPSEFGND D + AVEP
Sbjct: 99  DHESLVKAIKQVDVVISTVGHAQLADQGTIIAAIKEAGNVKRFFPSEFGNDVDRVHAVEP 158

Query: 124 AKSMYAAKAEFRRAIEAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILGDGTPK 183
           AK+ +A KA+ RR IEAEGIPHT+V  NFF GYFL  L+QP A+ PPR+KVVILGDG PK
Sbjct: 159 AKTAFATKAKIRRTIEAEGIPHTYVASNFFAGYFLPTLNQPGATAPPREKVVILGDGNPK 218

Query: 184 VIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDLVSLWERKIGKSLKRIYVPE 243
            I+NKEED+G YTI+A+DDPRTLNKI+Y+RPPAN  S NDLVSLWE+KIGK+L+RIYVPE
Sbjct: 219 AIFNKEEDIGAYTIKAVDDPRTLNKILYIRPPANTISFNDLVSLWEKKIGKTLERIYVPE 278

Query: 244 EEVLKKIRETSYPLNIELALCHTAQVKGCQTNFSIEPSFGVEASALYPDVRYTTVEEYLD 303
           E++LK I E + P+N+ L++ H+  VKG  TNF +EPSFGVEASALYPDV+YTTV+EYLD
Sbjct: 279 EQLLKNIEEAAVPINVILSIGHSVFVKGDHTNFEVEPSFGVEASALYPDVKYTTVDEYLD 338

Query: 304 QFV 307
           QFV
Sbjct: 339 QFV 341

[Close]

BLAST of Cucsa.109540 vs. TrEMBL
Match: V4SCG2_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10028904mg PE=4 SV=1)

HSP 1 Score: 448.0 bits (1151), Expect = 9.4e-123
Identity = 211/303 (69.64%), Postives = 261/303 (86.14%), Query Frame = 1


Query: 4   KVLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAKNRILNHFKSLGVNFLFGDLF 63
           K+L IGGTGYIGKFIV+AS KAGHPT+ L+R S+L +P+K+++L+HFK LGVN + GD+ 
Sbjct: 6   KILFIGGTGYIGKFIVEASVKAGHPTFVLVRESTLSAPSKSQLLDHFKKLGVNLVIGDVL 65

Query: 64  DNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHIDAVEP 123
           ++ESLVKAIKQVDVVIST+G  ++  Q KI++AIK+AGN+KRFFPSEFGND D + AVEP
Sbjct: 66  NHESLVKAIKQVDVVISTVGHTLLGDQVKIIAAIKEAGNIKRFFPSEFGNDVDRVHAVEP 125

Query: 124 AKSMYAAKAEFRRAIEAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILGDGTPK 183
           AKS +A KA+ RRA+EAEGIP+T+V  NFF  YFL NLSQP A+ PPRDKVVILGDG PK
Sbjct: 126 AKSAFATKAKIRRAVEAEGIPYTYVASNFFAAYFLPNLSQPGATAPPRDKVVILGDGNPK 185

Query: 184 VIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDLVSLWERKIGKSLKRIYVPE 243
            +YNKE+D+GTYTI+A+DDPRTLNKI+Y++PP NIYS NDLVSLWERKIGK+L+R+YVPE
Sbjct: 186 AVYNKEDDIGTYTIKAVDDPRTLNKILYIKPPGNIYSFNDLVSLWERKIGKTLERVYVPE 245

Query: 244 EEVLKKIRETSYPLNIELALCHTAQVKGCQTNFSIEPSFGVEASALYPDVRYTTVEEYLD 303
           E+VLK I+E + P+N+ L++ H+A VKG QTNF IEPSFGVEAS LYP V+YTTV+EYL 
Sbjct: 246 EQVLKNIQEAAPPVNVILSIQHSAFVKGDQTNFQIEPSFGVEASQLYPQVKYTTVDEYLS 305

Query: 304 QFV 307
           QFV
Sbjct: 306 QFV 308

[Close]

BLAST of Cucsa.109540 vs. TrEMBL
Match: A9PF66_POPTR (Putative uncharacterized protein OS=Populus trichocarpa PE=2 SV=1)

HSP 1 Score: 447.6 bits (1150), Expect = 1.2e-122
Identity = 213/306 (69.61%), Postives = 262/306 (85.62%), Query Frame = 1


Query: 1   MAQKVLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAKNRILNHFKSLGVNFLFG 60
           MA K+L IGGTGYIGKFIV+ASAKAGHPT+ L+R S+L +PAK+ ++++FK+LGVNFL G
Sbjct: 1   MASKILFIGGTGYIGKFIVEASAKAGHPTFVLVRESTLSNPAKSVVIDNFKNLGVNFLIG 60

Query: 61  DLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHIDA 120
           DLFD+ESLVKAIKQVDVVIST+G   +  Q +I++AIK+AGNVKRFFPSEFGND D ++A
Sbjct: 61  DLFDHESLVKAIKQVDVVISTVGHAQLVEQDRIIAAIKEAGNVKRFFPSEFGNDVDRVNA 120

Query: 121 VEPAKSMYAAKAEFRRAIEAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILGDG 180
           VEPAKS +A KA  RRAIEAEGIP+T+V  NFF GYFL + +QP A+ PPRDKVVILGDG
Sbjct: 121 VEPAKSAFATKANVRRAIEAEGIPYTYVSSNFFSGYFLLSFNQPGATAPPRDKVVILGDG 180

Query: 181 TPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDLVSLWERKIGKSLKRIY 240
            PK ++NKE+D+ TYTI+A+DDPRTLNKI+Y++PPAN  S NDLVSLWE+KIGK+L+RIY
Sbjct: 181 NPKAVFNKEDDIATYTIKAVDDPRTLNKILYIKPPANTISFNDLVSLWEKKIGKTLERIY 240

Query: 241 VPEEEVLKKIRETSYPLNIELALCHTAQVKGCQTNFSIEPSFGVEASALYPDVRYTTVEE 300
           VPEE++LK I+E S P+N+ L++ H+  VKG  TNF IEPSFGVEAS LYPDV+YTTV+E
Sbjct: 241 VPEEQLLKNIQEASVPVNVVLSIGHSVFVKGDHTNFEIEPSFGVEASELYPDVKYTTVDE 300

Query: 301 YLDQFV 307
           YL QFV
Sbjct: 301 YLKQFV 306

[Close]

BLAST of Cucsa.109540 vs. TAIR10
Match: AT4G39230.1 (AT4G39230.1 NmrA-like negative transcriptional regulator family protein)

HSP 1 Score: 432.2 bits (1110), Expect = 2.7e-121
Identity = 205/303 (67.66%), Postives = 253/303 (83.50%), Query Frame = 1


Query: 4   KVLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAKNRILNHFKSLGVNFLFGDLF 63
           K+L IGGTGYIGK+IV+ASA++GHPT  L+R S+L SP+++  + +FK+LGV FL GDL 
Sbjct: 6   KILFIGGTGYIGKYIVEASARSGHPTLVLVRNSTLTSPSRSSTIENFKNLGVQFLLGDLD 65

Query: 64  DNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHIDAVEP 123
           D+ SLV +IKQ DVVIST+G  ++ HQ+KI+SAIK+AGNVKRFFPSEFGND D +  VEP
Sbjct: 66  DHTSLVNSIKQADVVISTVGHSLLGHQYKIISAIKEAGNVKRFFPSEFGNDVDRVFTVEP 125

Query: 124 AKSMYAAKAEFRRAIEAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILGDGTPK 183
           AKS YA KA+ RR IEAEGIP+T+V CNFF GYFL  L+QP A+  PRDKV++LGDG PK
Sbjct: 126 AKSAYATKAKIRRTIEAEGIPYTYVSCNFFAGYFLPTLAQPGATSAPRDKVIVLGDGNPK 185

Query: 184 VIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDLVSLWERKIGKSLKRIYVPE 243
            ++NKEED+GTYTI A+DDPRTLNKI+Y+RPP N YS NDLVSLWE KIGK+L+RIYVPE
Sbjct: 186 AVFNKEEDIGTYTINAVDDPRTLNKILYIRPPMNTYSFNDLVSLWENKIGKTLERIYVPE 245

Query: 244 EEVLKKIRETSYPLNIELALCHTAQVKGCQTNFSIEPSFGVEASALYPDVRYTTVEEYLD 303
           E++LK+I E+S PLN+ L+LCH   VKG  T+F IEPSFGVEAS LYPDV+YTTV+E L+
Sbjct: 246 EQLLKQIIESSPPLNVMLSLCHCVFVKGGHTSFEIEPSFGVEASELYPDVKYTTVDEILN 305

Query: 304 QFV 307
           Q+V
Sbjct: 306 QYV 308

[Close]

BLAST of Cucsa.109540 vs. TAIR10
Match: AT1G75280.1 (AT1G75280.1 NmrA-like negative transcriptional regulator family protein)

HSP 1 Score: 375.6 bits (963), Expect = 3.0e-104
Identity = 185/304 (60.86%), Postives = 232/304 (76.32%), Query Frame = 1


Query: 4   KVLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAKNRILNHFKSLGVNFLFGDLF 63
           K+L+IGGTGYIGKF+V+ASAKAGH T+AL+R ++L  P K + +  FK LGV  L GDL 
Sbjct: 7   KILVIGGTGYIGKFLVEASAKAGHSTFALVREATLSDPVKGKTVQSFKDLGVTILHGDLN 66

Query: 64  DNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHIDAVEP 123
           D+ESLVKAIKQVDVVIST+G   +  Q KI+SAIK+AGNVKRF PSEFG D D   AVEP
Sbjct: 67  DHESLVKAIKQVDVVISTVGSMQILDQTKIISAIKEAGNVKRFLPSEFGVDVDRTSAVEP 126

Query: 124 AKSMYAAKAEFRRAIEAEGIPHTFVVCNFFDGYFLSNLSQ--PDASVPPRDKVVILGDGT 183
           AKS +A K + RR IEAEGIP+T+ V   F GY+L  L Q  P  + PPRDKV ILGDG 
Sbjct: 127 AKSAFAGKIQIRRTIEAEGIPYTYAVTGCFGGYYLPTLVQFEPGLTSPPRDKVTILGDGN 186

Query: 184 PKVIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDLVSLWERKIGKSLKRIYV 243
            K + NKEED+  YTI+A+DDPRTLNKI+Y++P  N  S N++V+LWE+KIGKSL++ ++
Sbjct: 187 AKAVINKEEDIAAYTIKAVDDPRTLNKILYIKPSNNTLSMNEIVTLWEKKIGKSLEKTHL 246

Query: 244 PEEEVLKKIRETSYPLNIELALCHTAQVKGCQTNFSIEPSFGVEASALYPDVRYTTVEEY 303
           PEE++LK I+E+  P+N+ L++ H   V G  TN SIEPSFGVEAS LYPDV+YT+V+EY
Sbjct: 247 PEEQLLKSIQESPIPINVVLSINHAVFVNG-DTNISIEPSFGVEASELYPDVKYTSVDEY 306

Query: 304 LDQF 306
           L  F
Sbjct: 307 LSYF 309

[Close]

BLAST of Cucsa.109540 vs. TAIR10
Match: AT1G75290.1 (AT1G75290.1 NAD(P)-binding Rossmann-fold superfamily protein)

HSP 1 Score: 368.2 bits (944), Expect = 4.8e-102
Identity = 178/304 (58.55%), Postives = 228/304 (75.00%), Query Frame = 1


Query: 4   KVLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAKNRILNHFKSLGVNFLFGDLF 63
           K+L+IGGTG+IGK I++AS KAGH T AL+R +SL  P K + + +FK  GV  L GDL 
Sbjct: 7   KILVIGGTGHIGKLIIEASVKAGHSTLALVREASLSDPNKGKTVQNFKDFGVTLLHGDLN 66

Query: 64  DNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHIDAVEP 123
           D+ESLVKAIKQ DVVIST+G   +  Q KI+SAIK+AGNVKRF PSEFG D D   AVEP
Sbjct: 67  DHESLVKAIKQADVVISTVGSMQILDQTKIISAIKEAGNVKRFLPSEFGMDVDKSSAVEP 126

Query: 124 AKSMYAAKAEFRRAIEAEGIPHTFVVCNFFDGYFLSNLSQ--PDASVPPRDKVVILGDGT 183
           AKS +  K + RR IEAEGIP+T++V N+F GY+L  L Q  P  + PPRDKV I GDG 
Sbjct: 127 AKSAFGRKLQTRRDIEAEGIPYTYLVTNYFAGYYLPTLVQLEPGLTSPPRDKVKIFGDGN 186

Query: 184 PKVIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDLVSLWERKIGKSLKRIYV 243
            K + NKEED+  YTI+A+DDPRTLNK +Y+ PP N  S N++V+LWE+KIGKS+++IY+
Sbjct: 187 VKAVINKEEDIAAYTIKAVDDPRTLNKTLYINPPNNTLSMNEIVTLWEKKIGKSVEKIYM 246

Query: 244 PEEEVLKKIRETSYPLNIELALCHTAQVKGCQTNFSIEPSFGVEASALYPDVRYTTVEEY 303
            EE++ K I+E+  P N+ L++ H   VKG QTNF+IEPSFG EAS LYPD++YT+++EY
Sbjct: 247 SEEQIFKSIQESPVPFNVLLSINHAVFVKGDQTNFTIEPSFGFEASELYPDIKYTSIDEY 306

Query: 304 LDQF 306
           L  F
Sbjct: 307 LSYF 310

[Close]

BLAST of Cucsa.109540 vs. TAIR10
Match: AT1G75300.1 (AT1G75300.1 NmrA-like negative transcriptional regulator family protein)

HSP 1 Score: 357.5 bits (916), Expect = 8.5e-99
Identity = 180/318 (56.60%), Postives = 234/318 (73.58%), Query Frame = 1


Query: 4   KVLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAKNRILNHFKSLGVNFLFGDLF 63
           K+L+IGGTGY+G+FIV+ SAKAG+PT+AL+R +SL  P K++ +  FK LGV  L GDL 
Sbjct: 7   KILVIGGTGYMGEFIVEGSAKAGNPTFALVREASLSDPVKSKTIQSFKDLGVTILHGDLN 66

Query: 64  DNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHIDAVEP 123
           D+ESLVKAIKQVDVVIST+G   +  Q KI+SAIK+AGNVKRF P+EFG D +   AVEP
Sbjct: 67  DHESLVKAIKQVDVVISTIGHKQIFDQTKIISAIKEAGNVKRFLPAEFGIDVERTSAVEP 126

Query: 124 AKSMYAAKAEFRRAIEAEGIPHTFVVCNFFDGYFLSNLSQ----------------PDAS 183
           AKS++A K + RRAIEAEGIP+T+VV N   G++L  L Q                 D +
Sbjct: 127 AKSLFAGKVQIRRAIEAEGIPYTYVVSNCSAGFYLRTLLQFESGLISHTRDKAIIFGDKN 186

Query: 184 VPPRDKVVILGDGTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDLVSL 243
           VPPRDKV ILGDG  KV+ NKEEDV  Y I+A+DD RTLNK +Y+ PP NI S N++V+L
Sbjct: 187 VPPRDKVTILGDGNAKVVINKEEDVAAYMIKAVDDLRTLNKTLYISPPNNILSMNEMVTL 246

Query: 244 WERKIGKSLKRIYVPEEEVLKKIRETSYPLNIELALCHTAQVKGCQTNFSIEPSFGVEAS 303
           WE+KIGKSL++ ++ EE++LK I+    P+++  ++ H   VKG QT+F+IEP FG EAS
Sbjct: 247 WEKKIGKSLEKTHISEEQILKSIQ---VPIDVFKSINHAVFVKGDQTSFTIEPWFGEEAS 306

Query: 304 ALYPDVRYTTVEEYLDQF 306
            LYPDV+YT+++EYL QF
Sbjct: 307 VLYPDVKYTSIDEYLSQF 321

[Close]

BLAST of Cucsa.109540 vs. TAIR10
Match: AT1G19540.1 (AT1G19540.1 NmrA-like negative transcriptional regulator family protein)

HSP 1 Score: 329.7 bits (844), Expect = 1.9e-90
Identity = 168/310 (54.19%), Postives = 220/310 (70.97%), Query Frame = 1


Query: 1   MAQKVLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAKNRILNHFKSLGVNFLFG 60
           M  K+L+IG TG IGK +V+ SAK+GH T+AL+R +SL  P K +++  FK LGV  L+G
Sbjct: 1   MTSKILVIGATGLIGKVLVEESAKSGHATFALVREASLSDPVKAQLVERFKDLGVTILYG 60

Query: 61  DLFDNESLVKAIKQVDVVISTLGGHM--VPHQHKILSAIKQAGNVKRFFPSEFGNDADHI 120
            L D ESLVKAIKQVDVVIS +G     + +Q  I+ AIK++GNVKRF PSEFGND D  
Sbjct: 61  SLSDKESLVKAIKQVDVVISAVGRFQTEILNQTNIIDAIKESGNVKRFLPSEFGNDVDRT 120

Query: 121 DAVEPAKSMYAAKAEFRRAIEAEGIPHTFVVCNFFDGYFLSNLSQPDASV--PPRDKVVI 180
            A+EP  S +  KA+ RRAIEA  IP+T+VV   F G F+  L Q    +  PPRDKV I
Sbjct: 121 VAIEPTLSEFITKAQIRRAIEAAKIPYTYVVSGCFAGLFVPCLGQCHLRLRSPPRDKVSI 180

Query: 181 LGDGTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDLVSLWERKIGKSL 240
              G  K I N EED+  YT++A+DDPRTLNKI+Y+ PP  I S ND+V LWE KIGK+L
Sbjct: 181 YDTGNGKAIVNTEEDIVAYTLKAVDDPRTLNKILYIHPPNYIVSQNDMVGLWEEKIGKTL 240

Query: 241 KRIYVPEEEVLKKIRETSYPLNIELALCHTAQVKGCQTNFSIEPSFGVEASALYPDVRYT 300
           ++ YV EEE+LK I+E+  P++  + L HT  VK   T+F+I+PSFGVEAS LYP+V+YT
Sbjct: 241 EKTYVSEEELLKTIQESKPPMDFLVGLIHTILVKSDFTSFTIDPSFGVEASELYPEVKYT 300

Query: 301 TVEEYLDQFV 307
           +V+E+L++F+
Sbjct: 301 SVDEFLNRFI 310

[Close]

BLAST of Cucsa.109540 vs. NCBI nr
Match: gi|449462218|ref|XP_004148838.1| (PREDICTED: isoflavone reductase-like protein [Cucumis sativus])

HSP 1 Score: 613.2 bits (1580), Expect = 2.4e-172
Identity = 306/306 (100.00%), Postives = 306/306 (100.00%), Query Frame = 1


Query: 1   MAQKVLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAKNRILNHFKSLGVNFLFG 60
           MAQKVLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAKNRILNHFKSLGVNFLFG
Sbjct: 1   MAQKVLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAKNRILNHFKSLGVNFLFG 60

Query: 61  DLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHIDA 120
           DLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHIDA
Sbjct: 61  DLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHIDA 120

Query: 121 VEPAKSMYAAKAEFRRAIEAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILGDG 180
           VEPAKSMYAAKAEFRRAIEAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILGDG
Sbjct: 121 VEPAKSMYAAKAEFRRAIEAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILGDG 180

Query: 181 TPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDLVSLWERKIGKSLKRIY 240
           TPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDLVSLWERKIGKSLKRIY
Sbjct: 181 TPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDLVSLWERKIGKSLKRIY 240

Query: 241 VPEEEVLKKIRETSYPLNIELALCHTAQVKGCQTNFSIEPSFGVEASALYPDVRYTTVEE 300
           VPEEEVLKKIRETSYPLNIELALCHTAQVKGCQTNFSIEPSFGVEASALYPDVRYTTVEE
Sbjct: 241 VPEEEVLKKIRETSYPLNIELALCHTAQVKGCQTNFSIEPSFGVEASALYPDVRYTTVEE 300

Query: 301 YLDQFV 307
           YLDQFV
Sbjct: 301 YLDQFV 306

[Close]

BLAST of Cucsa.109540 vs. NCBI nr
Match: gi|659122052|ref|XP_008460945.1| (PREDICTED: isoflavone reductase-like protein [Cucumis melo])

HSP 1 Score: 606.3 bits (1562), Expect = 3.0e-170
Identity = 302/306 (98.69%), Postives = 304/306 (99.35%), Query Frame = 1


Query: 1   MAQKVLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAKNRILNHFKSLGVNFLFG 60
           MAQKVLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAKNRILNHFKSLGVNFLFG
Sbjct: 1   MAQKVLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAKNRILNHFKSLGVNFLFG 60

Query: 61  DLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHIDA 120
           DLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHIDA
Sbjct: 61  DLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHIDA 120

Query: 121 VEPAKSMYAAKAEFRRAIEAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILGDG 180
           VEPAKSMY+AKAEFRRA+EAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILGDG
Sbjct: 121 VEPAKSMYSAKAEFRRAVEAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILGDG 180

Query: 181 TPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDLVSLWERKIGKSLKRIY 240
           TPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDLVSLWERKIGKSLKRIY
Sbjct: 181 TPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDLVSLWERKIGKSLKRIY 240

Query: 241 VPEEEVLKKIRETSYPLNIELALCHTAQVKGCQTNFSIEPSFGVEASALYPDVRYTTVEE 300
           VPEEEVLKKIRETSYPLNIELALCHTAQVKG QTNF IEPSFGVEASALYPDVRYTTVEE
Sbjct: 241 VPEEEVLKKIRETSYPLNIELALCHTAQVKGSQTNFDIEPSFGVEASALYPDVRYTTVEE 300

Query: 301 YLDQFV 307
           YLDQFV
Sbjct: 301 YLDQFV 306

[Close]

BLAST of Cucsa.109540 vs. NCBI nr
Match: gi|778669354|ref|XP_004148840.2| (PREDICTED: isoflavone reductase-like protein [Cucumis sativus])

HSP 1 Score: 453.4 bits (1165), Expect = 3.2e-124
Identity = 220/308 (71.43%), Postives = 265/308 (86.04%), Query Frame = 1


Query: 1   MAQ--KVLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAKNRILNHFKSLGVNFL 60
           MAQ  KVL+IG TGYIGKF+V+ASA+AGHPT+AL+R+S+L   AK+ I+++F++LGVNF+
Sbjct: 18  MAQNLKVLVIGATGYIGKFVVEASAQAGHPTFALVRQSTLADSAKSSIIHNFRNLGVNFV 77

Query: 61  FGDLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHI 120
           FGD+FDNESLV+AI+QVDVVIST+G  ++ HQ KI+SAIKQAGNVKRF PSEFGND D +
Sbjct: 78  FGDIFDNESLVRAIQQVDVVISTVGRGLLSHQEKIISAIKQAGNVKRFLPSEFGNDVDRV 137

Query: 121 DAVEPAKSMYAAKAEFRRAIEAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILG 180
            AVEPAKSM+A+K E RRA+EAEGIPHTFVV NFFDGY+L N SQP A+ PPRDK+ I G
Sbjct: 138 HAVEPAKSMFASKVEIRRAVEAEGIPHTFVVSNFFDGYYLRNFSQPGATEPPRDKIKIFG 197

Query: 181 DGTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDLVSLWERKIGKSLKR 240
           DG  KVIYNKEED+GTYTIRAIDDPRT NKI+Y+RPPANIYSTN+LVSLWE+KIG+ L+R
Sbjct: 198 DGNLKVIYNKEEDIGTYTIRAIDDPRTFNKILYIRPPANIYSTNELVSLWEKKIGRILER 257

Query: 241 IYVPEEEVLKKIRETSYPLNIELALCHTAQVKGCQTNFSIEPSFGVEASALYPDVRYTTV 300
            YV EEE++K I+ET  PL+  LA+ H+A VKG  TNF IEPS GVEAS LYP+V YTTV
Sbjct: 258 TYVSEEELVKNIQETPVPLSTALAISHSAFVKGDHTNFEIEPSIGVEASELYPNVHYTTV 317

Query: 301 EEYLDQFV 307
           E+YL+QFV
Sbjct: 318 EDYLNQFV 325

[Close]

BLAST of Cucsa.109540 vs. NCBI nr
Match: gi|700206685|gb|KGN61804.1| (hypothetical protein Csa_2G248135 [Cucumis sativus])

HSP 1 Score: 453.4 bits (1165), Expect = 3.2e-124
Identity = 220/308 (71.43%), Postives = 265/308 (86.04%), Query Frame = 1


Query: 1   MAQ--KVLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAKNRILNHFKSLGVNFL 60
           MAQ  KVL+IG TGYIGKF+V+ASA+AGHPT+AL+R+S+L   AK+ I+++F++LGVNF+
Sbjct: 1   MAQNLKVLVIGATGYIGKFVVEASAQAGHPTFALVRQSTLADSAKSSIIHNFRNLGVNFV 60

Query: 61  FGDLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHI 120
           FGD+FDNESLV+AI+QVDVVIST+G  ++ HQ KI+SAIKQAGNVKRF PSEFGND D +
Sbjct: 61  FGDIFDNESLVRAIQQVDVVISTVGRGLLSHQEKIISAIKQAGNVKRFLPSEFGNDVDRV 120

Query: 121 DAVEPAKSMYAAKAEFRRAIEAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILG 180
            AVEPAKSM+A+K E RRA+EAEGIPHTFVV NFFDGY+L N SQP A+ PPRDK+ I G
Sbjct: 121 HAVEPAKSMFASKVEIRRAVEAEGIPHTFVVSNFFDGYYLRNFSQPGATEPPRDKIKIFG 180

Query: 181 DGTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDLVSLWERKIGKSLKR 240
           DG  KVIYNKEED+GTYTIRAIDDPRT NKI+Y+RPPANIYSTN+LVSLWE+KIG+ L+R
Sbjct: 181 DGNLKVIYNKEEDIGTYTIRAIDDPRTFNKILYIRPPANIYSTNELVSLWEKKIGRILER 240

Query: 241 IYVPEEEVLKKIRETSYPLNIELALCHTAQVKGCQTNFSIEPSFGVEASALYPDVRYTTV 300
            YV EEE++K I+ET  PL+  LA+ H+A VKG  TNF IEPS GVEAS LYP+V YTTV
Sbjct: 241 TYVSEEELVKNIQETPVPLSTALAISHSAFVKGDHTNFEIEPSIGVEASELYPNVHYTTV 300

Query: 301 EEYLDQFV 307
           E+YL+QFV
Sbjct: 301 EDYLNQFV 308

[Close]

BLAST of Cucsa.109540 vs. NCBI nr
Match: gi|659122054|ref|XP_008460946.1| (PREDICTED: isoflavone reductase homolog [Cucumis melo])

HSP 1 Score: 451.1 bits (1159), Expect = 1.6e-123
Identity = 219/308 (71.10%), Postives = 261/308 (84.74%), Query Frame = 1


Query: 1   MAQK--VLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAKNRILNHFKSLGVNFL 60
           MAQK  VLIIGGTGYIGKF+V+ASA+AGHPT+ L+R+S+L   AK+ I++ F++LGVNF+
Sbjct: 1   MAQKLKVLIIGGTGYIGKFVVEASAQAGHPTFVLVRQSTLSDSAKSSIIDSFRNLGVNFV 60

Query: 61  FGDLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHI 120
           FGD+FDNESLV+AI+QVDVVIST+G  ++ HQ  I+SAIKQAGN+KRFFPSEFGND D +
Sbjct: 61  FGDIFDNESLVRAIQQVDVVISTVGRGLLSHQQNIISAIKQAGNIKRFFPSEFGNDVDRV 120

Query: 121 DAVEPAKSMYAAKAEFRRAIEAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILG 180
            AVEPAKSM+A K E RR +EAEGIPHTFVV NFFDGY+L N SQP A+ PPRDK+ I G
Sbjct: 121 HAVEPAKSMFARKVEIRRVVEAEGIPHTFVVSNFFDGYYLRNFSQPGATEPPRDKIKIFG 180

Query: 181 DGTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDLVSLWERKIGKSLKR 240
           DG  KVIYNKEED+GTYTIRAIDDPRT NKI+Y+RPPANIYSTN+LVSLWE+KI + L++
Sbjct: 181 DGNLKVIYNKEEDIGTYTIRAIDDPRTFNKILYIRPPANIYSTNELVSLWEKKICRILEK 240

Query: 241 IYVPEEEVLKKIRETSYPLNIELALCHTAQVKGCQTNFSIEPSFGVEASALYPDVRYTTV 300
            YV EEE++K I+ET  PLN  LA+ H+A VKG  TNF IEPS GVEAS LYPDV YTTV
Sbjct: 241 TYVSEEELVKNIQETPVPLNTALAISHSAFVKGDHTNFDIEPSIGVEASELYPDVHYTTV 300

Query: 301 EEYLDQFV 307
           E+YL+QFV
Sbjct: 301 EDYLNQFV 308

The following BLAST results are available for this feature:

BLAST of Cucsa.109540 vs. Swiss-Prot


Match Name	E-value	Identity	Description
ALL12_OLEEU	5.5e-116	66.34	Isoflavone reductase-like protein OS=Olea europaea PE=1 SV=1	[more]
IFRH_SOLTU	1.5e-113	64.03	Isoflavone reductase homolog OS=Solanum tuberosum PE=2 SV=1	[more]
IFRH_TOBAC	1.0e-106	59.74	Isoflavone reductase homolog A622 OS=Nicotiana tabacum PE=2 SV=1	[more]
IFRH_ARATH	5.4e-103	60.86	Isoflavone reductase homolog P3 OS=Arabidopsis thaliana GN=At1g75280 PE=2 SV=1	[more]
IFRH_MAIZE	1.9e-92	54.79	Isoflavone reductase homolog IRL OS=Zea mays GN=IRL PE=2 SV=1	[more]

BLAST of Cucsa.109540 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LJ01_CUCSA	1.7e-172	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G248130 PE=4 SV=1	[more]
A0A0A0LL65_CUCSA	2.2e-124	71.43	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G248135 PE=4 SV=1	[more]
M5VMM7_PRUPE	7.2e-123	70.30	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa008220mg PE=4 SV=1	[more]
V4SCG2_9ROSI	9.4e-123	69.64	Uncharacterized protein OS=Citrus clementina GN=CICLE_v10028904mg PE=4 SV=1	[more]
A9PF66_POPTR	1.2e-122	69.61	Putative uncharacterized protein OS=Populus trichocarpa PE=2 SV=1	[more]

BLAST of Cucsa.109540 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G39230.1	2.7e-121	67.66	NmrA-like negative transcriptional regulator family protein	[more]
AT1G75280.1	3.0e-104	60.86	NmrA-like negative transcriptional regulator family protein	[more]
AT1G75290.1	4.8e-102	58.55	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT1G75300.1	8.5e-99	56.60	NmrA-like negative transcriptional regulator family protein	[more]
AT1G19540.1	1.9e-90	54.19	NmrA-like negative transcriptional regulator family protein	[more]

BLAST of Cucsa.109540 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449462218\|ref\|XP_004148838.1\|	2.4e-172	100.00	PREDICTED: isoflavone reductase-like protein [Cucumis sativus]	[more]
gi\|659122052\|ref\|XP_008460945.1\|	3.0e-170	98.69	PREDICTED: isoflavone reductase-like protein [Cucumis melo]	[more]
gi\|778669354\|ref\|XP_004148840.2\|	3.2e-124	71.43	PREDICTED: isoflavone reductase-like protein [Cucumis sativus]	[more]
gi\|700206685\|gb\|KGN61804.1\|	3.2e-124	71.43	hypothetical protein Csa_2G248135 [Cucumis sativus]	[more]
gi\|659122054\|ref\|XP_008460946.1\|	1.6e-123	71.10	PREDICTED: isoflavone reductase homolog [Cucumis melo]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR008030	NmrA-like
IPR016040	NAD(P)-bd_dom

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsa.109540.1	Cucsa.109540.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008030	NmrA-like domain	PFAM	PF05368	NmrA	coord: 5..293 score: 4.6
IPR016040	NAD(P)-binding domain	GENE3D	G3DSA:3.40.50.720		coord: 3..96 score: 1.2
IPR016040	NAD(P)-binding domain	unknown	SSF51735	NAD(P)-binding Rossmann-fold domains	coord: 4..253 score: 2.5
None	No IPR available	GENE3D	G3DSA:3.90.25.10		coord: 150..306 score: 6.3
None	No IPR available	PANTHER	PTHR10366	NAD DEPENDENT EPIMERASE/DEHYDRATASE	coord: 266..306 score: 4.0E-157coord: 4..244 score: 4.0E
None	No IPR available	PANTHER	PTHR10366:SF375	SUBFAMILY NOT NAMED	coord: 266..306 score: 4.0E-157coord: 4..244 score: 4.0E

Synteny

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Cucsa.109540	Cucumber (Chinese Long) v3	cgycucB138
Cucsa.109540	Cucumber (Chinese Long) v3	cgycucB139
Cucsa.109540	Cucumber (Chinese Long) v3	cgycucB141
Cucsa.109540	Watermelon (97103) v2	cgywmbB150
Cucsa.109540	Watermelon (97103) v2	cgywmbB151
Cucsa.109540	Wax gourd	cgywgoB172
Cucsa.109540	Wax gourd	cgywgoB173
Cucsa.109540	Cucumber (Gy14) v1	cgycgyB058
Cucsa.109540	Cucurbita maxima (Rimu)	cgycmaB0253
Cucsa.109540	Cucurbita maxima (Rimu)	cgycmaB0254
Cucsa.109540	Cucurbita moschata (Rifu)	cgycmoB0251
Cucsa.109540	Cucurbita moschata (Rifu)	cgycmoB0252
Cucsa.109540	Cucurbita moschata (Rifu)	cgycmoB0253
Cucsa.109540	Wild cucumber (PI 183967)	cgycpiB134
Cucsa.109540	Cucumber (Chinese Long) v2	cgycuB133
Cucsa.109540	Melon (DHL92) v3.5.1	cgymeB148
Cucsa.109540	Melon (DHL92) v3.5.1	cgymeB151
Cucsa.109540	Watermelon (Charleston Gray)	cgywcgB155
Cucsa.109540	Watermelon (Charleston Gray)	cgywcgB156
Cucsa.109540	Watermelon (97103) v1	cgywmB155
Cucsa.109540	Watermelon (97103) v1	cgywmB156
Cucsa.109540	Cucurbita pepo (Zucchini)	cgycpeB0245
Cucsa.109540	Cucurbita pepo (Zucchini)	cgycpeB0246
Cucsa.109540	Cucurbita pepo (Zucchini)	cgycpeB0247
Cucsa.109540	Bottle gourd (USVL1VR-Ls)	cgylsiB145
Cucsa.109540	Bottle gourd (USVL1VR-Ls)	cgylsiB146
Cucsa.109540	Melon (DHL92) v3.6.1	cgymedB148
Cucsa.109540	Melon (DHL92) v3.6.1	cgymedB150
Cucsa.109540	Silver-seed gourd	carcgyB0368
Cucsa.109540	Silver-seed gourd	carcgyB0542
Cucsa.109540	Silver-seed gourd	carcgyB1012