Cucsa.109540.1 (mRNA) Cucumber (Gy14) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cucsa.109540.1
Type	mRNA
Organism	Cucumis sativus (Cucumber (Gy14) v1)
Description	Isoflavone reductase family protein
Location	scaffold00934 : 464260 .. 466594 (-)
Sequence length	1182

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TCCCACCCACCGGTCAAAGCAAATATTTTTTAACAACGACAAAGGCACTCCACGGTACTCCTCCGATCAACTATTTCTCCGCCGGGAACTCCTTACTCCGATGGCTCAGAAGGTTCTTATCATCGGAGGAACTGGCTACATCGGTAAATTCATCGTACAAGCCAGCGCCAAAGCTGGTCATCCTACATATGCATTGATCCGCAGGTCTTCGCTCGAAAGCCCCGCCAAGAATCGAATCCTCAATCATTTCAAAAGCTTAGGAGTCAATTTTCTCTTCGTAAGTACATTTCTCTGGATTTTTTTTGTTTCATTGCTCATAATTATTTTGTAATCCTGATTTTCTAGGTCTGATTTGATTTTAGTCGTGGTTACTTATAATGTAGCGTACTGTTGATTCTTTTGAGTTATTCACATTATTAGTTCTTGAATTCTGTGGAACCATAATGAATTTGATTGGATATGGTATTGTTTAATTTGGCTGAAACTATGAAAATTGGGACAGGGTGATCTCTTTGACAATGAGAGTTTGGTTAAGGCGATTAAGCAGGTTGATGTGGTGATATCAACCCTTGGTGGCCATATGGTTCCCCATCAACATAAAATCCTATCTGCTATCAAGCAAGCTGGCAATGTGAAGGTAATCTTATTCTACATTTTCCTTACAACTATGTCATTGATTGTGATTGTTTTGCTGCAATATCTATTTTTCTAGTAGAGTTTGTGCGTTTTGGGAATTTTGATCATTGGAATTTGTGTGCCACAGAGGTTCTTTCCTTCTGAGTTCGGTAACGATGCGGATCATATCGATGCAGTGGAACCAGCGAAATCTATGTATGCTGCAAAGGCTGAATTTCGTAGGGCTATTGAAGCTGAGGGAATCCCACATACATTTGTGGTATGCAATTTCTTCGATGGTTACTTTCTTAGCAACTTGTCTCAGCCAGATGCAAGCGTCCCACCTAGAGATAAAGTCGTTATCTTAGGAGATGGAACTCCCAAAGGTACAATATGTCATTAAATTTATCTTCCCCATCAGCTCAAGTTTTTAAGTAAAATGGTAAATTGACATTGTTATCAGAATCTAGAAAAAAAATCATGTGTTTAAACTTCTCCAGTGTTATTTCCTTCCTAATTAATATTGATTTCACTTGTTGGACTTTGTACATATTTCAATCTCACAAGAGAGAGTTAGATGATATAACATTAAATCTACCTTCATCCATGAGTGTAAGCTTTTAGTCAATTGCCGATTCGAAATTATCTTTATTCTCATGAGTTCTAATTGAATCTTTTTTTATTGTGGGATAAATTGTGGTACATACCATATCCTATTTTCTTGAGTTGAATGGTTTCCAAACCTTCCTTGGAAATCAAATAGATAATGATGCATTTCATTTGGCTTTTTATTCATCAAACCAATTGCTGTATATTATTGTCTTCAATATGGTGTTTCAATAGCCTTCCACGCTATTCATTCTGTATCGGATTTGTTTGTCAAAATAGCGATTTGCATCAAGGAAGAAGCTCTTCTAATGAACAAAACTTCTGCATATATACACACGTGTTATAAATAATTGATTGCAACATGAACCATCATTCATACATCGTACTTAGTTTCTTGAAGGGAGATATAGAATTTGTTTCAACAGCCATTCACCCTATTCATTATGTCTTTGTTATGTTTCATGAAGTCATTTACAACAAGGAAGAAGATGTTGGAACGTACACAATCCGAGCAATAGATGATCCAAGAACCCTCAACAAGATCATGTACCTTAGGCCCCCAGCTAACATCTACTCAACCAATGATCTCGTGTCGCTGTGGGAGAGAAAAATTGGCAAGAGTCTTAAAAGAATTTATGTACCAGAGGAAGAAGTATTGAAGAAAATCCGAGGTGGGTTTTTTAAGCAAAGCAACAAACTTTCAATTCTTATATTCTATTGTATGCTTCTAATCACAAACTGACTAACAACACAATTGCTTTCGCTAATCATTTCGTTTTCCAGAGACTTCATATCCACTTAATATAGAATTGGCACTATGCCACACTGCTCAAGTGAAGGGATGTCAAACTAATTTTAGTATTGAACCATCTTTTGGAGTTGAAGCTTCAGCATTGTACCCAGATGTTCGGTATACAACTGTAGAAGAATACCTCGACCAGTTTGTCTAAAGAAAAAGTCTTTTGTTTTTGTTTTTTTTTTTTTTTTACTTTTGTCATTTTGGCATAATGGGTGTAAGTCTTAGGCCGTTGGGTGTTATGTATTCATGTATAATAAGGAAATGCTCTTATTTGAAAATAGAGGTTCTTACATAATTCAAAGTTTAGAATGA

mRNA sequence

TCCCACCCACCGGTCAAAGCAAATATTTTTTAACAACGACAAAGGCACTCCACGGTACTCCTCCGATCAACTATTTCTCCGCCGGGAACTCCTTACTCCGATGGCTCAGAAGGTTCTTATCATCGGAGGAACTGGCTACATCGGTAAATTCATCGTACAAGCCAGCGCCAAAGCTGGTCATCCTACATATGCATTGATCCGCAGGTCTTCGCTCGAAAGCCCCGCCAAGAATCGAATCCTCAATCATTTCAAAAGCTTAGGAGTCAATTTTCTCTTCGGTGATCTCTTTGACAATGAGAGTTTGGTTAAGGCGATTAAGCAGGTTGATGTGGTGATATCAACCCTTGGTGGCCATATGGTTCCCCATCAACATAAAATCCTATCTGCTATCAAGCAAGCTGGCAATGTGAAGAGGTTCTTTCCTTCTGAGTTCGGTAACGATGCGGATCATATCGATGCAGTGGAACCAGCGAAATCTATGTATGCTGCAAAGGCTGAATTTCGTAGGGCTATTGAAGCTGAGGGAATCCCACATACATTTGTGGTATGCAATTTCTTCGATGGTTACTTTCTTAGCAACTTGTCTCAGCCAGATGCAAGCGTCCCACCTAGAGATAAAGTCGTTATCTTAGGAGATGGAACTCCCAAAGTCATTTACAACAAGGAAGAAGATGTTGGAACGTACACAATCCGAGCAATAGATGATCCAAGAACCCTCAACAAGATCATGTACCTTAGGCCCCCAGCTAACATCTACTCAACCAATGATCTCGTGTCGCTGTGGGAGAGAAAAATTGGCAAGAGTCTTAAAAGAATTTATGTACCAGAGGAAGAAGTATTGAAGAAAATCCGAGAGACTTCATATCCACTTAATATAGAATTGGCACTATGCCACACTGCTCAAGTGAAGGGATGTCAAACTAATTTTAGTATTGAACCATCTTTTGGAGTTGAAGCTTCAGCATTGTACCCAGATGTTCGGTATACAACTGTAGAAGAATACCTCGACCAGTTTGTCTAAAGAAAAAGTCttttgtttttgttttttttttttttttacttttgtCATTTTGGCATAATGGGTGTAAGTCTTAGGCCGTTGGGTGTTATGTATTCATGTATAATAAGGAAATGCTCTTATTTGAAAATAGAGGTTCTTACATAATTCAAAGTTTAGAATGA

Coding sequence (CDS)

ATGGCTCAGAAGGTTCTTATCATCGGAGGAACTGGCTACATCGGTAAATTCATCGTACAAGCCAGCGCCAAAGCTGGTCATCCTACATATGCATTGATCCGCAGGTCTTCGCTCGAAAGCCCCGCCAAGAATCGAATCCTCAATCATTTCAAAAGCTTAGGAGTCAATTTTCTCTTCGGTGATCTCTTTGACAATGAGAGTTTGGTTAAGGCGATTAAGCAGGTTGATGTGGTGATATCAACCCTTGGTGGCCATATGGTTCCCCATCAACATAAAATCCTATCTGCTATCAAGCAAGCTGGCAATGTGAAGAGGTTCTTTCCTTCTGAGTTCGGTAACGATGCGGATCATATCGATGCAGTGGAACCAGCGAAATCTATGTATGCTGCAAAGGCTGAATTTCGTAGGGCTATTGAAGCTGAGGGAATCCCACATACATTTGTGGTATGCAATTTCTTCGATGGTTACTTTCTTAGCAACTTGTCTCAGCCAGATGCAAGCGTCCCACCTAGAGATAAAGTCGTTATCTTAGGAGATGGAACTCCCAAAGTCATTTACAACAAGGAAGAAGATGTTGGAACGTACACAATCCGAGCAATAGATGATCCAAGAACCCTCAACAAGATCATGTACCTTAGGCCCCCAGCTAACATCTACTCAACCAATGATCTCGTGTCGCTGTGGGAGAGAAAAATTGGCAAGAGTCTTAAAAGAATTTATGTACCAGAGGAAGAAGTATTGAAGAAAATCCGAGAGACTTCATATCCACTTAATATAGAATTGGCACTATGCCACACTGCTCAAGTGAAGGGATGTCAAACTAATTTTAGTATTGAACCATCTTTTGGAGTTGAAGCTTCAGCATTGTACCCAGATGTTCGGTATACAACTGTAGAAGAATACCTCGACCAGTTTGTCTAA

Protein sequence

MAQKVLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAKNRILNHFKSLGVNFLFGDLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHIDAVEPAKSMYAAKAEFRRAIEAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILGDGTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDLVSLWERKIGKSLKRIYVPEEEVLKKIRETSYPLNIELALCHTAQVKGCQTNFSIEPSFGVEASALYPDVRYTTVEEYLDQFV*

Homology

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BLAST of Cucsa.109540.1 vs. Swiss-Prot
Match: ALL12_OLEEU (Isoflavone reductase-like protein OS=Olea europaea PE=1 SV=1)

HSP 1 Score: 418.7 bits (1075), Expect = 5.5e-116
Identity = 201/303 (66.34%), Postives = 251/303 (82.84%), Query Frame = 1


Query: 4   KVLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAKNRILNHFKSLGVNFLFGDLF 63
           K+LIIGGTGYIGKFIV+ASAK+ HPT+AL R S++  P K +I+  FK+ GV  L GDL+
Sbjct: 6   KILIIGGTGYIGKFIVEASAKSEHPTFALARESTISDPVKGKIIQGFKNSGVTILTGDLY 65

Query: 64  DNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHIDAVEP 123
           D+ESLVKAIKQVDVVIST+G   +  Q KI++AIK+AGNVKRFFPS+FG D D   AVEP
Sbjct: 66  DHESLVKAIKQVDVVISTVGQLQLADQVKIIAAIKEAGNVKRFFPSDFGTDVDRCHAVEP 125

Query: 124 AKSMYAAKAEFRRAIEAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILGDGTPK 183
           AKS +  K++ RRAIEAEGIP+TFV  N+F GY L  L QP+ + PPRDKV+ILGDG  K
Sbjct: 126 AKSSFEIKSQIRRAIEAEGIPYTFVSANYFAGYSLPTLVQPEVTAPPRDKVIILGDGNAK 185

Query: 184 VIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDLVSLWERKIGKSLKRIYVPE 243
            ++N+E D+GTYTI+A+DD RTLNKI+Y++PP NIYS N+LV+LWE+KIGK+L++IYVPE
Sbjct: 186 AVFNEENDIGTYTIKAVDDARTLNKILYIKPPKNIYSFNELVALWEKKIGKTLEKIYVPE 245

Query: 244 EEVLKKIRETSYPLNIELALCHTAQVKGCQTNFSIEPSFGVEASALYPDVRYTTVEEYLD 303
           E+VLK+I+E+ +P+NI +A+ H+A VKG  TNF IEPSFGVEAS LYPDV+YTTVEEYLD
Sbjct: 246 EQVLKQIQESPFPINIVMAINHSAFVKGDLTNFKIEPSFGVEASELYPDVKYTTVEEYLD 305

Query: 304 QFV 307
           QFV
Sbjct: 306 QFV 308

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BLAST of Cucsa.109540.1 vs. Swiss-Prot
Match: IFRH_SOLTU (Isoflavone reductase homolog OS=Solanum tuberosum PE=2 SV=1)

HSP 1 Score: 410.6 bits (1054), Expect = 1.5e-113
Identity = 194/303 (64.03%), Postives = 248/303 (81.85%), Query Frame = 1


Query: 4   KVLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAKNRILNHFKSLGVNFLFGDLF 63
           K+L IGGTGYIGKFIV+ASAKAGH T+ L+R S+L +P K ++++ FKS GV F+ GDL+
Sbjct: 6   KILFIGGTGYIGKFIVEASAKAGHDTFVLVRESTLSNPTKTKLIDTFKSFGVTFVHGDLY 65

Query: 64  DNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHIDAVEP 123
           D+ESLVKAIKQVDVVIST+G  ++  Q K+++AIK+AGNVKRFFPSEFGND D + AVEP
Sbjct: 66  DHESLVKAIKQVDVVISTVGHALLADQVKLIAAIKEAGNVKRFFPSEFGNDVDRVHAVEP 125

Query: 124 AKSMYAAKAEFRRAIEAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILGDGTPK 183
           AK+ +  KA+ RR +EAEGIP T+V   FF GY L NL+QP A+ PP DKVVILG G  K
Sbjct: 126 AKAAFNTKAQIRRVVEAEGIPFTYVATFFFAGYSLPNLAQPGAAGPPNDKVVILGHGNTK 185

Query: 184 VIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDLVSLWERKIGKSLKRIYVPE 243
            ++NKEED+GTYTI A+DDP+TLNKI+Y++PP NI + N+LVSLWE+K GK+L+R+YVPE
Sbjct: 186 AVFNKEEDIGTYTINAVDDPKTLNKILYIKPPHNIITLNELVSLWEKKTGKNLERLYVPE 245

Query: 244 EEVLKKIRETSYPLNIELALCHTAQVKGCQTNFSIEPSFGVEASALYPDVRYTTVEEYLD 303
           E+VLK I+E S P+N+ L++ HTA VKG  TNF IEPSFGVEAS +YPDV+YT ++E L+
Sbjct: 246 EQVLKNIQEASVPMNVGLSIYHTAFVKGDHTNFEIEPSFGVEASEVYPDVKYTPIDEILN 305

Query: 304 QFV 307
           Q+V
Sbjct: 306 QYV 308

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BLAST of Cucsa.109540.1 vs. Swiss-Prot
Match: IFRH_TOBAC (Isoflavone reductase homolog A622 OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 387.9 bits (995), Expect = 1.0e-106
Identity = 181/303 (59.74%), Postives = 238/303 (78.55%), Query Frame = 1


Query: 4   KVLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAKNRILNHFKSLGVNFLFGDLF 63
           K+LIIGGTGYIGK++V+ SAK+GHPT+ALIR S+L++P K+++++ FKS GV  LFGD+ 
Sbjct: 8   KILIIGGTGYIGKYLVETSAKSGHPTFALIRESTLKNPEKSKLIDTFKSYGVTLLFGDIS 67

Query: 64  DNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHIDAVEP 123
           + ESL+KAIKQVDVVIST+GG     Q  I+ AIK+AGN+KRF PSEFG D DH  A+EP
Sbjct: 68  NQESLLKAIKQVDVVISTVGGQQFTDQVNIIKAIKEAGNIKRFLPSEFGFDVDHARAIEP 127

Query: 124 AKSMYAAKAEFRRAIEAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILGDGTPK 183
           A S++A K   RR IEAEGIP+T+V+CN+F  +FL NL Q +A  PPRDKVVI GDG PK
Sbjct: 128 AASLFALKVRIRRMIEAEGIPYTYVICNWFADFFLPNLGQLEAKTPPRDKVVIFGDGNPK 187

Query: 184 VIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDLVSLWERKIGKSLKRIYVPE 243
            IY KEED+ TYTI A+DDPRTLNK +++RPPANI S N++VSLWE KIGK+L+++Y+ E
Sbjct: 188 AIYVKEEDIATYTIEAVDDPRTLNKTLHMRPPANILSFNEIVSLWEDKIGKTLEKLYLSE 247

Query: 244 EEVLKKIRETSYPLNIELALCHTAQVKGCQTNFSIEPSFGVEASALYPDVRYTTVEEYLD 303
           E++L+ ++E   PL   LA+CH+  V G   NF ++P  GVEA+ LYP V+YTTV+E+ +
Sbjct: 248 EDILQIVQEGPLPLRTNLAICHSVFVNGDSANFEVQPPTGVEATELYPKVKYTTVDEFYN 307

Query: 304 QFV 307
           +FV
Sbjct: 308 KFV 310

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BLAST of Cucsa.109540.1 vs. Swiss-Prot
Match: IFRH_ARATH (Isoflavone reductase homolog P3 OS=Arabidopsis thaliana GN=At1g75280 PE=2 SV=1)

HSP 1 Score: 375.6 bits (963), Expect = 5.4e-103
Identity = 185/304 (60.86%), Postives = 232/304 (76.32%), Query Frame = 1


Query: 4   KVLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAKNRILNHFKSLGVNFLFGDLF 63
           K+L+IGGTGYIGKF+V+ASAKAGH T+AL+R ++L  P K + +  FK LGV  L GDL 
Sbjct: 7   KILVIGGTGYIGKFLVEASAKAGHSTFALVREATLSDPVKGKTVQSFKDLGVTILHGDLN 66

Query: 64  DNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHIDAVEP 123
           D+ESLVKAIKQVDVVIST+G   +  Q KI+SAIK+AGNVKRF PSEFG D D   AVEP
Sbjct: 67  DHESLVKAIKQVDVVISTVGSMQILDQTKIISAIKEAGNVKRFLPSEFGVDVDRTSAVEP 126

Query: 124 AKSMYAAKAEFRRAIEAEGIPHTFVVCNFFDGYFLSNLSQ--PDASVPPRDKVVILGDGT 183
           AKS +A K + RR IEAEGIP+T+ V   F GY+L  L Q  P  + PPRDKV ILGDG 
Sbjct: 127 AKSAFAGKIQIRRTIEAEGIPYTYAVTGCFGGYYLPTLVQFEPGLTSPPRDKVTILGDGN 186

Query: 184 PKVIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDLVSLWERKIGKSLKRIYV 243
            K + NKEED+  YTI+A+DDPRTLNKI+Y++P  N  S N++V+LWE+KIGKSL++ ++
Sbjct: 187 AKAVINKEEDIAAYTIKAVDDPRTLNKILYIKPSNNTLSMNEIVTLWEKKIGKSLEKTHL 246

Query: 244 PEEEVLKKIRETSYPLNIELALCHTAQVKGCQTNFSIEPSFGVEASALYPDVRYTTVEEY 303
           PEE++LK I+E+  P+N+ L++ H   V G  TN SIEPSFGVEAS LYPDV+YT+V+EY
Sbjct: 247 PEEQLLKSIQESPIPINVVLSINHAVFVNG-DTNISIEPSFGVEASELYPDVKYTSVDEY 306

Query: 304 LDQF 306
           L  F
Sbjct: 307 LSYF 309

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BLAST of Cucsa.109540.1 vs. Swiss-Prot
Match: IFRH_MAIZE (Isoflavone reductase homolog IRL OS=Zea mays GN=IRL PE=2 SV=1)

HSP 1 Score: 340.5 bits (872), Expect = 1.9e-92
Identity = 166/303 (54.79%), Postives = 218/303 (71.95%), Query Frame = 1


Query: 4   KVLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAKNRILNHFKSLGVNFLFGDLF 63
           K+L++GGTGY+G+ +V ASA+ GHPT AL+R ++   PAK  +L  F+  GV  L GDL+
Sbjct: 7   KILVVGGTGYLGRHVVAASARLGHPTSALVRDTAPSDPAKAALLKSFQDAGVTLLKGDLY 66

Query: 64  DNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHIDAVEP 123
           D  SLV A+K  DVVIS LG   +  Q +++ AIK+AGNVKRFFPSEFG D D    VEP
Sbjct: 67  DQASLVSAVKGADVVISVLGSMQIADQSRLVDAIKEAGNVKRFFPSEFGLDVDRTGIVEP 126

Query: 124 AKSMYAAKAEFRRAIEAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILGDGTPK 183
           AKS+  AK   RRA EA GIP+T+ V  FF G+ L  + Q  A  PP DK V+LGDG  K
Sbjct: 127 AKSILGAKVGIRRATEAAGIPYTYAVAGFFAGFGLPKVGQVLAPGPPADKAVVLGDGDTK 186

Query: 184 VIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDLVSLWERKIGKSLKRIYVPE 243
            ++ +E D+ TYT+ A DDPR  NK++Y++PPAN  S N+L+SLWE+K GK+ +R YVPE
Sbjct: 187 AVFVEEGDIATYTVLAADDPRAENKVLYIKPPANTLSHNELLSLWEKKTGKTFRREYVPE 246

Query: 244 EEVLKKIRETSYPLNIELALCHTAQVKGCQTNFSIEPSFGVEASALYPDVRYTTVEEYLD 303
           E VLK+I+E+  PLNI LA+ H A V+G QT F I+P+ GV+AS LYPDV+YTTV+EYL+
Sbjct: 247 EAVLKQIQESPIPLNIILAIGHAAFVRGEQTGFEIDPAKGVDASELYPDVKYTTVDEYLN 306

Query: 304 QFV 307
           +F+
Sbjct: 307 RFL 309

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BLAST of Cucsa.109540.1 vs. TrEMBL
Match: A0A0A0LJ01_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G248130 PE=4 SV=1)

HSP 1 Score: 613.2 bits (1580), Expect = 1.7e-172
Identity = 306/306 (100.00%), Postives = 306/306 (100.00%), Query Frame = 1


Query: 1   MAQKVLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAKNRILNHFKSLGVNFLFG 60
           MAQKVLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAKNRILNHFKSLGVNFLFG
Sbjct: 1   MAQKVLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAKNRILNHFKSLGVNFLFG 60

Query: 61  DLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHIDA 120
           DLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHIDA
Sbjct: 61  DLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHIDA 120

Query: 121 VEPAKSMYAAKAEFRRAIEAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILGDG 180
           VEPAKSMYAAKAEFRRAIEAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILGDG
Sbjct: 121 VEPAKSMYAAKAEFRRAIEAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILGDG 180

Query: 181 TPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDLVSLWERKIGKSLKRIY 240
           TPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDLVSLWERKIGKSLKRIY
Sbjct: 181 TPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDLVSLWERKIGKSLKRIY 240

Query: 241 VPEEEVLKKIRETSYPLNIELALCHTAQVKGCQTNFSIEPSFGVEASALYPDVRYTTVEE 300
           VPEEEVLKKIRETSYPLNIELALCHTAQVKGCQTNFSIEPSFGVEASALYPDVRYTTVEE
Sbjct: 241 VPEEEVLKKIRETSYPLNIELALCHTAQVKGCQTNFSIEPSFGVEASALYPDVRYTTVEE 300

Query: 301 YLDQFV 307
           YLDQFV
Sbjct: 301 YLDQFV 306

[Close]

BLAST of Cucsa.109540.1 vs. TrEMBL
Match: A0A0A0LL65_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G248135 PE=4 SV=1)

HSP 1 Score: 453.4 bits (1165), Expect = 2.2e-124
Identity = 220/308 (71.43%), Postives = 265/308 (86.04%), Query Frame = 1


Query: 1   MAQ--KVLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAKNRILNHFKSLGVNFL 60
           MAQ  KVL+IG TGYIGKF+V+ASA+AGHPT+AL+R+S+L   AK+ I+++F++LGVNF+
Sbjct: 1   MAQNLKVLVIGATGYIGKFVVEASAQAGHPTFALVRQSTLADSAKSSIIHNFRNLGVNFV 60

Query: 61  FGDLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHI 120
           FGD+FDNESLV+AI+QVDVVIST+G  ++ HQ KI+SAIKQAGNVKRF PSEFGND D +
Sbjct: 61  FGDIFDNESLVRAIQQVDVVISTVGRGLLSHQEKIISAIKQAGNVKRFLPSEFGNDVDRV 120

Query: 121 DAVEPAKSMYAAKAEFRRAIEAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILG 180
            AVEPAKSM+A+K E RRA+EAEGIPHTFVV NFFDGY+L N SQP A+ PPRDK+ I G
Sbjct: 121 HAVEPAKSMFASKVEIRRAVEAEGIPHTFVVSNFFDGYYLRNFSQPGATEPPRDKIKIFG 180

Query: 181 DGTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDLVSLWERKIGKSLKR 240
           DG  KVIYNKEED+GTYTIRAIDDPRT NKI+Y+RPPANIYSTN+LVSLWE+KIG+ L+R
Sbjct: 181 DGNLKVIYNKEEDIGTYTIRAIDDPRTFNKILYIRPPANIYSTNELVSLWEKKIGRILER 240

Query: 241 IYVPEEEVLKKIRETSYPLNIELALCHTAQVKGCQTNFSIEPSFGVEASALYPDVRYTTV 300
            YV EEE++K I+ET  PL+  LA+ H+A VKG  TNF IEPS GVEAS LYP+V YTTV
Sbjct: 241 TYVSEEELVKNIQETPVPLSTALAISHSAFVKGDHTNFEIEPSIGVEASELYPNVHYTTV 300

Query: 301 EEYLDQFV 307
           E+YL+QFV
Sbjct: 301 EDYLNQFV 308

[Close]

BLAST of Cucsa.109540.1 vs. TrEMBL
Match: M5VMM7_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa008220mg PE=4 SV=1)

HSP 1 Score: 448.4 bits (1152), Expect = 7.2e-123
Identity = 213/303 (70.30%), Postives = 258/303 (85.15%), Query Frame = 1


Query: 4   KVLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAKNRILNHFKSLGVNFLFGDLF 63
           K+L IGGTGYIGKFIV+ASAKAGHPTYAL+R  +L +PAK +++ +FKSLGVNF+ GDL+
Sbjct: 39  KILFIGGTGYIGKFIVEASAKAGHPTYALVREPTLSNPAKAKVIENFKSLGVNFVLGDLY 98

Query: 64  DNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHIDAVEP 123
           D+ESLVKAIKQVDVVIST+G   +  Q  I++AIK+AGNVKRFFPSEFGND D + AVEP
Sbjct: 99  DHESLVKAIKQVDVVISTVGHAQLADQGTIIAAIKEAGNVKRFFPSEFGNDVDRVHAVEP 158

Query: 124 AKSMYAAKAEFRRAIEAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILGDGTPK 183
           AK+ +A KA+ RR IEAEGIPHT+V  NFF GYFL  L+QP A+ PPR+KVVILGDG PK
Sbjct: 159 AKTAFATKAKIRRTIEAEGIPHTYVASNFFAGYFLPTLNQPGATAPPREKVVILGDGNPK 218

Query: 184 VIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDLVSLWERKIGKSLKRIYVPE 243
            I+NKEED+G YTI+A+DDPRTLNKI+Y+RPPAN  S NDLVSLWE+KIGK+L+RIYVPE
Sbjct: 219 AIFNKEEDIGAYTIKAVDDPRTLNKILYIRPPANTISFNDLVSLWEKKIGKTLERIYVPE 278

Query: 244 EEVLKKIRETSYPLNIELALCHTAQVKGCQTNFSIEPSFGVEASALYPDVRYTTVEEYLD 303
           E++LK I E + P+N+ L++ H+  VKG  TNF +EPSFGVEASALYPDV+YTTV+EYLD
Sbjct: 279 EQLLKNIEEAAVPINVILSIGHSVFVKGDHTNFEVEPSFGVEASALYPDVKYTTVDEYLD 338

Query: 304 QFV 307
           QFV
Sbjct: 339 QFV 341

[Close]

BLAST of Cucsa.109540.1 vs. TrEMBL
Match: V4SCG2_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10028904mg PE=4 SV=1)

HSP 1 Score: 448.0 bits (1151), Expect = 9.4e-123
Identity = 211/303 (69.64%), Postives = 261/303 (86.14%), Query Frame = 1


Query: 4   KVLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAKNRILNHFKSLGVNFLFGDLF 63
           K+L IGGTGYIGKFIV+AS KAGHPT+ L+R S+L +P+K+++L+HFK LGVN + GD+ 
Sbjct: 6   KILFIGGTGYIGKFIVEASVKAGHPTFVLVRESTLSAPSKSQLLDHFKKLGVNLVIGDVL 65

Query: 64  DNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHIDAVEP 123
           ++ESLVKAIKQVDVVIST+G  ++  Q KI++AIK+AGN+KRFFPSEFGND D + AVEP
Sbjct: 66  NHESLVKAIKQVDVVISTVGHTLLGDQVKIIAAIKEAGNIKRFFPSEFGNDVDRVHAVEP 125

Query: 124 AKSMYAAKAEFRRAIEAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILGDGTPK 183
           AKS +A KA+ RRA+EAEGIP+T+V  NFF  YFL NLSQP A+ PPRDKVVILGDG PK
Sbjct: 126 AKSAFATKAKIRRAVEAEGIPYTYVASNFFAAYFLPNLSQPGATAPPRDKVVILGDGNPK 185

Query: 184 VIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDLVSLWERKIGKSLKRIYVPE 243
            +YNKE+D+GTYTI+A+DDPRTLNKI+Y++PP NIYS NDLVSLWERKIGK+L+R+YVPE
Sbjct: 186 AVYNKEDDIGTYTIKAVDDPRTLNKILYIKPPGNIYSFNDLVSLWERKIGKTLERVYVPE 245

Query: 244 EEVLKKIRETSYPLNIELALCHTAQVKGCQTNFSIEPSFGVEASALYPDVRYTTVEEYLD 303
           E+VLK I+E + P+N+ L++ H+A VKG QTNF IEPSFGVEAS LYP V+YTTV+EYL 
Sbjct: 246 EQVLKNIQEAAPPVNVILSIQHSAFVKGDQTNFQIEPSFGVEASQLYPQVKYTTVDEYLS 305

Query: 304 QFV 307
           QFV
Sbjct: 306 QFV 308

[Close]

BLAST of Cucsa.109540.1 vs. TrEMBL
Match: A9PF66_POPTR (Putative uncharacterized protein OS=Populus trichocarpa PE=2 SV=1)

HSP 1 Score: 447.6 bits (1150), Expect = 1.2e-122
Identity = 213/306 (69.61%), Postives = 262/306 (85.62%), Query Frame = 1


Query: 1   MAQKVLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAKNRILNHFKSLGVNFLFG 60
           MA K+L IGGTGYIGKFIV+ASAKAGHPT+ L+R S+L +PAK+ ++++FK+LGVNFL G
Sbjct: 1   MASKILFIGGTGYIGKFIVEASAKAGHPTFVLVRESTLSNPAKSVVIDNFKNLGVNFLIG 60

Query: 61  DLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHIDA 120
           DLFD+ESLVKAIKQVDVVIST+G   +  Q +I++AIK+AGNVKRFFPSEFGND D ++A
Sbjct: 61  DLFDHESLVKAIKQVDVVISTVGHAQLVEQDRIIAAIKEAGNVKRFFPSEFGNDVDRVNA 120

Query: 121 VEPAKSMYAAKAEFRRAIEAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILGDG 180
           VEPAKS +A KA  RRAIEAEGIP+T+V  NFF GYFL + +QP A+ PPRDKVVILGDG
Sbjct: 121 VEPAKSAFATKANVRRAIEAEGIPYTYVSSNFFSGYFLLSFNQPGATAPPRDKVVILGDG 180

Query: 181 TPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDLVSLWERKIGKSLKRIY 240
            PK ++NKE+D+ TYTI+A+DDPRTLNKI+Y++PPAN  S NDLVSLWE+KIGK+L+RIY
Sbjct: 181 NPKAVFNKEDDIATYTIKAVDDPRTLNKILYIKPPANTISFNDLVSLWEKKIGKTLERIY 240

Query: 241 VPEEEVLKKIRETSYPLNIELALCHTAQVKGCQTNFSIEPSFGVEASALYPDVRYTTVEE 300
           VPEE++LK I+E S P+N+ L++ H+  VKG  TNF IEPSFGVEAS LYPDV+YTTV+E
Sbjct: 241 VPEEQLLKNIQEASVPVNVVLSIGHSVFVKGDHTNFEIEPSFGVEASELYPDVKYTTVDE 300

Query: 301 YLDQFV 307
           YL QFV
Sbjct: 301 YLKQFV 306

[Close]

BLAST of Cucsa.109540.1 vs. TAIR10
Match: AT4G39230.1 (AT4G39230.1 NmrA-like negative transcriptional regulator family protein)

HSP 1 Score: 432.2 bits (1110), Expect = 2.7e-121
Identity = 205/303 (67.66%), Postives = 253/303 (83.50%), Query Frame = 1


Query: 4   KVLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAKNRILNHFKSLGVNFLFGDLF 63
           K+L IGGTGYIGK+IV+ASA++GHPT  L+R S+L SP+++  + +FK+LGV FL GDL 
Sbjct: 6   KILFIGGTGYIGKYIVEASARSGHPTLVLVRNSTLTSPSRSSTIENFKNLGVQFLLGDLD 65

Query: 64  DNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHIDAVEP 123
           D+ SLV +IKQ DVVIST+G  ++ HQ+KI+SAIK+AGNVKRFFPSEFGND D +  VEP
Sbjct: 66  DHTSLVNSIKQADVVISTVGHSLLGHQYKIISAIKEAGNVKRFFPSEFGNDVDRVFTVEP 125

Query: 124 AKSMYAAKAEFRRAIEAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILGDGTPK 183
           AKS YA KA+ RR IEAEGIP+T+V CNFF GYFL  L+QP A+  PRDKV++LGDG PK
Sbjct: 126 AKSAYATKAKIRRTIEAEGIPYTYVSCNFFAGYFLPTLAQPGATSAPRDKVIVLGDGNPK 185

Query: 184 VIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDLVSLWERKIGKSLKRIYVPE 243
            ++NKEED+GTYTI A+DDPRTLNKI+Y+RPP N YS NDLVSLWE KIGK+L+RIYVPE
Sbjct: 186 AVFNKEEDIGTYTINAVDDPRTLNKILYIRPPMNTYSFNDLVSLWENKIGKTLERIYVPE 245

Query: 244 EEVLKKIRETSYPLNIELALCHTAQVKGCQTNFSIEPSFGVEASALYPDVRYTTVEEYLD 303
           E++LK+I E+S PLN+ L+LCH   VKG  T+F IEPSFGVEAS LYPDV+YTTV+E L+
Sbjct: 246 EQLLKQIIESSPPLNVMLSLCHCVFVKGGHTSFEIEPSFGVEASELYPDVKYTTVDEILN 305

Query: 304 QFV 307
           Q+V
Sbjct: 306 QYV 308

[Close]

BLAST of Cucsa.109540.1 vs. TAIR10
Match: AT1G75280.1 (AT1G75280.1 NmrA-like negative transcriptional regulator family protein)

HSP 1 Score: 375.6 bits (963), Expect = 3.0e-104
Identity = 185/304 (60.86%), Postives = 232/304 (76.32%), Query Frame = 1


Query: 4   KVLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAKNRILNHFKSLGVNFLFGDLF 63
           K+L+IGGTGYIGKF+V+ASAKAGH T+AL+R ++L  P K + +  FK LGV  L GDL 
Sbjct: 7   KILVIGGTGYIGKFLVEASAKAGHSTFALVREATLSDPVKGKTVQSFKDLGVTILHGDLN 66

Query: 64  DNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHIDAVEP 123
           D+ESLVKAIKQVDVVIST+G   +  Q KI+SAIK+AGNVKRF PSEFG D D   AVEP
Sbjct: 67  DHESLVKAIKQVDVVISTVGSMQILDQTKIISAIKEAGNVKRFLPSEFGVDVDRTSAVEP 126

Query: 124 AKSMYAAKAEFRRAIEAEGIPHTFVVCNFFDGYFLSNLSQ--PDASVPPRDKVVILGDGT 183
           AKS +A K + RR IEAEGIP+T+ V   F GY+L  L Q  P  + PPRDKV ILGDG 
Sbjct: 127 AKSAFAGKIQIRRTIEAEGIPYTYAVTGCFGGYYLPTLVQFEPGLTSPPRDKVTILGDGN 186

Query: 184 PKVIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDLVSLWERKIGKSLKRIYV 243
            K + NKEED+  YTI+A+DDPRTLNKI+Y++P  N  S N++V+LWE+KIGKSL++ ++
Sbjct: 187 AKAVINKEEDIAAYTIKAVDDPRTLNKILYIKPSNNTLSMNEIVTLWEKKIGKSLEKTHL 246

Query: 244 PEEEVLKKIRETSYPLNIELALCHTAQVKGCQTNFSIEPSFGVEASALYPDVRYTTVEEY 303
           PEE++LK I+E+  P+N+ L++ H   V G  TN SIEPSFGVEAS LYPDV+YT+V+EY
Sbjct: 247 PEEQLLKSIQESPIPINVVLSINHAVFVNG-DTNISIEPSFGVEASELYPDVKYTSVDEY 306

Query: 304 LDQF 306
           L  F
Sbjct: 307 LSYF 309

[Close]

BLAST of Cucsa.109540.1 vs. TAIR10
Match: AT1G75290.1 (AT1G75290.1 NAD(P)-binding Rossmann-fold superfamily protein)

HSP 1 Score: 368.2 bits (944), Expect = 4.8e-102
Identity = 178/304 (58.55%), Postives = 228/304 (75.00%), Query Frame = 1


Query: 4   KVLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAKNRILNHFKSLGVNFLFGDLF 63
           K+L+IGGTG+IGK I++AS KAGH T AL+R +SL  P K + + +FK  GV  L GDL 
Sbjct: 7   KILVIGGTGHIGKLIIEASVKAGHSTLALVREASLSDPNKGKTVQNFKDFGVTLLHGDLN 66

Query: 64  DNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHIDAVEP 123
           D+ESLVKAIKQ DVVIST+G   +  Q KI+SAIK+AGNVKRF PSEFG D D   AVEP
Sbjct: 67  DHESLVKAIKQADVVISTVGSMQILDQTKIISAIKEAGNVKRFLPSEFGMDVDKSSAVEP 126

Query: 124 AKSMYAAKAEFRRAIEAEGIPHTFVVCNFFDGYFLSNLSQ--PDASVPPRDKVVILGDGT 183
           AKS +  K + RR IEAEGIP+T++V N+F GY+L  L Q  P  + PPRDKV I GDG 
Sbjct: 127 AKSAFGRKLQTRRDIEAEGIPYTYLVTNYFAGYYLPTLVQLEPGLTSPPRDKVKIFGDGN 186

Query: 184 PKVIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDLVSLWERKIGKSLKRIYV 243
            K + NKEED+  YTI+A+DDPRTLNK +Y+ PP N  S N++V+LWE+KIGKS+++IY+
Sbjct: 187 VKAVINKEEDIAAYTIKAVDDPRTLNKTLYINPPNNTLSMNEIVTLWEKKIGKSVEKIYM 246

Query: 244 PEEEVLKKIRETSYPLNIELALCHTAQVKGCQTNFSIEPSFGVEASALYPDVRYTTVEEY 303
            EE++ K I+E+  P N+ L++ H   VKG QTNF+IEPSFG EAS LYPD++YT+++EY
Sbjct: 247 SEEQIFKSIQESPVPFNVLLSINHAVFVKGDQTNFTIEPSFGFEASELYPDIKYTSIDEY 306

Query: 304 LDQF 306
           L  F
Sbjct: 307 LSYF 310

[Close]

BLAST of Cucsa.109540.1 vs. TAIR10
Match: AT1G75300.1 (AT1G75300.1 NmrA-like negative transcriptional regulator family protein)

HSP 1 Score: 357.5 bits (916), Expect = 8.5e-99
Identity = 180/318 (56.60%), Postives = 234/318 (73.58%), Query Frame = 1


Query: 4   KVLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAKNRILNHFKSLGVNFLFGDLF 63
           K+L+IGGTGY+G+FIV+ SAKAG+PT+AL+R +SL  P K++ +  FK LGV  L GDL 
Sbjct: 7   KILVIGGTGYMGEFIVEGSAKAGNPTFALVREASLSDPVKSKTIQSFKDLGVTILHGDLN 66

Query: 64  DNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHIDAVEP 123
           D+ESLVKAIKQVDVVIST+G   +  Q KI+SAIK+AGNVKRF P+EFG D +   AVEP
Sbjct: 67  DHESLVKAIKQVDVVISTIGHKQIFDQTKIISAIKEAGNVKRFLPAEFGIDVERTSAVEP 126

Query: 124 AKSMYAAKAEFRRAIEAEGIPHTFVVCNFFDGYFLSNLSQ----------------PDAS 183
           AKS++A K + RRAIEAEGIP+T+VV N   G++L  L Q                 D +
Sbjct: 127 AKSLFAGKVQIRRAIEAEGIPYTYVVSNCSAGFYLRTLLQFESGLISHTRDKAIIFGDKN 186

Query: 184 VPPRDKVVILGDGTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDLVSL 243
           VPPRDKV ILGDG  KV+ NKEEDV  Y I+A+DD RTLNK +Y+ PP NI S N++V+L
Sbjct: 187 VPPRDKVTILGDGNAKVVINKEEDVAAYMIKAVDDLRTLNKTLYISPPNNILSMNEMVTL 246

Query: 244 WERKIGKSLKRIYVPEEEVLKKIRETSYPLNIELALCHTAQVKGCQTNFSIEPSFGVEAS 303
           WE+KIGKSL++ ++ EE++LK I+    P+++  ++ H   VKG QT+F+IEP FG EAS
Sbjct: 247 WEKKIGKSLEKTHISEEQILKSIQ---VPIDVFKSINHAVFVKGDQTSFTIEPWFGEEAS 306

Query: 304 ALYPDVRYTTVEEYLDQF 306
            LYPDV+YT+++EYL QF
Sbjct: 307 VLYPDVKYTSIDEYLSQF 321

[Close]

BLAST of Cucsa.109540.1 vs. TAIR10
Match: AT1G19540.1 (AT1G19540.1 NmrA-like negative transcriptional regulator family protein)

HSP 1 Score: 329.7 bits (844), Expect = 1.9e-90
Identity = 168/310 (54.19%), Postives = 220/310 (70.97%), Query Frame = 1


Query: 1   MAQKVLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAKNRILNHFKSLGVNFLFG 60
           M  K+L+IG TG IGK +V+ SAK+GH T+AL+R +SL  P K +++  FK LGV  L+G
Sbjct: 1   MTSKILVIGATGLIGKVLVEESAKSGHATFALVREASLSDPVKAQLVERFKDLGVTILYG 60

Query: 61  DLFDNESLVKAIKQVDVVISTLGGHM--VPHQHKILSAIKQAGNVKRFFPSEFGNDADHI 120
            L D ESLVKAIKQVDVVIS +G     + +Q  I+ AIK++GNVKRF PSEFGND D  
Sbjct: 61  SLSDKESLVKAIKQVDVVISAVGRFQTEILNQTNIIDAIKESGNVKRFLPSEFGNDVDRT 120

Query: 121 DAVEPAKSMYAAKAEFRRAIEAEGIPHTFVVCNFFDGYFLSNLSQPDASV--PPRDKVVI 180
            A+EP  S +  KA+ RRAIEA  IP+T+VV   F G F+  L Q    +  PPRDKV I
Sbjct: 121 VAIEPTLSEFITKAQIRRAIEAAKIPYTYVVSGCFAGLFVPCLGQCHLRLRSPPRDKVSI 180

Query: 181 LGDGTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDLVSLWERKIGKSL 240
              G  K I N EED+  YT++A+DDPRTLNKI+Y+ PP  I S ND+V LWE KIGK+L
Sbjct: 181 YDTGNGKAIVNTEEDIVAYTLKAVDDPRTLNKILYIHPPNYIVSQNDMVGLWEEKIGKTL 240

Query: 241 KRIYVPEEEVLKKIRETSYPLNIELALCHTAQVKGCQTNFSIEPSFGVEASALYPDVRYT 300
           ++ YV EEE+LK I+E+  P++  + L HT  VK   T+F+I+PSFGVEAS LYP+V+YT
Sbjct: 241 EKTYVSEEELLKTIQESKPPMDFLVGLIHTILVKSDFTSFTIDPSFGVEASELYPEVKYT 300

Query: 301 TVEEYLDQFV 307
           +V+E+L++F+
Sbjct: 301 SVDEFLNRFI 310

[Close]

BLAST of Cucsa.109540.1 vs. NCBI nr
Match: gi|449462218|ref|XP_004148838.1| (PREDICTED: isoflavone reductase-like protein [Cucumis sativus])

HSP 1 Score: 613.2 bits (1580), Expect = 2.4e-172
Identity = 306/306 (100.00%), Postives = 306/306 (100.00%), Query Frame = 1


Query: 1   MAQKVLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAKNRILNHFKSLGVNFLFG 60
           MAQKVLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAKNRILNHFKSLGVNFLFG
Sbjct: 1   MAQKVLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAKNRILNHFKSLGVNFLFG 60

Query: 61  DLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHIDA 120
           DLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHIDA
Sbjct: 61  DLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHIDA 120

Query: 121 VEPAKSMYAAKAEFRRAIEAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILGDG 180
           VEPAKSMYAAKAEFRRAIEAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILGDG
Sbjct: 121 VEPAKSMYAAKAEFRRAIEAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILGDG 180

Query: 181 TPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDLVSLWERKIGKSLKRIY 240
           TPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDLVSLWERKIGKSLKRIY
Sbjct: 181 TPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDLVSLWERKIGKSLKRIY 240

Query: 241 VPEEEVLKKIRETSYPLNIELALCHTAQVKGCQTNFSIEPSFGVEASALYPDVRYTTVEE 300
           VPEEEVLKKIRETSYPLNIELALCHTAQVKGCQTNFSIEPSFGVEASALYPDVRYTTVEE
Sbjct: 241 VPEEEVLKKIRETSYPLNIELALCHTAQVKGCQTNFSIEPSFGVEASALYPDVRYTTVEE 300

Query: 301 YLDQFV 307
           YLDQFV
Sbjct: 301 YLDQFV 306

[Close]

BLAST of Cucsa.109540.1 vs. NCBI nr
Match: gi|659122052|ref|XP_008460945.1| (PREDICTED: isoflavone reductase-like protein [Cucumis melo])

HSP 1 Score: 606.3 bits (1562), Expect = 3.0e-170
Identity = 302/306 (98.69%), Postives = 304/306 (99.35%), Query Frame = 1


Query: 1   MAQKVLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAKNRILNHFKSLGVNFLFG 60
           MAQKVLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAKNRILNHFKSLGVNFLFG
Sbjct: 1   MAQKVLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAKNRILNHFKSLGVNFLFG 60

Query: 61  DLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHIDA 120
           DLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHIDA
Sbjct: 61  DLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHIDA 120

Query: 121 VEPAKSMYAAKAEFRRAIEAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILGDG 180
           VEPAKSMY+AKAEFRRA+EAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILGDG
Sbjct: 121 VEPAKSMYSAKAEFRRAVEAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILGDG 180

Query: 181 TPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDLVSLWERKIGKSLKRIY 240
           TPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDLVSLWERKIGKSLKRIY
Sbjct: 181 TPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDLVSLWERKIGKSLKRIY 240

Query: 241 VPEEEVLKKIRETSYPLNIELALCHTAQVKGCQTNFSIEPSFGVEASALYPDVRYTTVEE 300
           VPEEEVLKKIRETSYPLNIELALCHTAQVKG QTNF IEPSFGVEASALYPDVRYTTVEE
Sbjct: 241 VPEEEVLKKIRETSYPLNIELALCHTAQVKGSQTNFDIEPSFGVEASALYPDVRYTTVEE 300

Query: 301 YLDQFV 307
           YLDQFV
Sbjct: 301 YLDQFV 306

[Close]

BLAST of Cucsa.109540.1 vs. NCBI nr
Match: gi|778669354|ref|XP_004148840.2| (PREDICTED: isoflavone reductase-like protein [Cucumis sativus])

HSP 1 Score: 453.4 bits (1165), Expect = 3.2e-124
Identity = 220/308 (71.43%), Postives = 265/308 (86.04%), Query Frame = 1


Query: 1   MAQ--KVLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAKNRILNHFKSLGVNFL 60
           MAQ  KVL+IG TGYIGKF+V+ASA+AGHPT+AL+R+S+L   AK+ I+++F++LGVNF+
Sbjct: 18  MAQNLKVLVIGATGYIGKFVVEASAQAGHPTFALVRQSTLADSAKSSIIHNFRNLGVNFV 77

Query: 61  FGDLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHI 120
           FGD+FDNESLV+AI+QVDVVIST+G  ++ HQ KI+SAIKQAGNVKRF PSEFGND D +
Sbjct: 78  FGDIFDNESLVRAIQQVDVVISTVGRGLLSHQEKIISAIKQAGNVKRFLPSEFGNDVDRV 137

Query: 121 DAVEPAKSMYAAKAEFRRAIEAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILG 180
            AVEPAKSM+A+K E RRA+EAEGIPHTFVV NFFDGY+L N SQP A+ PPRDK+ I G
Sbjct: 138 HAVEPAKSMFASKVEIRRAVEAEGIPHTFVVSNFFDGYYLRNFSQPGATEPPRDKIKIFG 197

Query: 181 DGTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDLVSLWERKIGKSLKR 240
           DG  KVIYNKEED+GTYTIRAIDDPRT NKI+Y+RPPANIYSTN+LVSLWE+KIG+ L+R
Sbjct: 198 DGNLKVIYNKEEDIGTYTIRAIDDPRTFNKILYIRPPANIYSTNELVSLWEKKIGRILER 257

Query: 241 IYVPEEEVLKKIRETSYPLNIELALCHTAQVKGCQTNFSIEPSFGVEASALYPDVRYTTV 300
            YV EEE++K I+ET  PL+  LA+ H+A VKG  TNF IEPS GVEAS LYP+V YTTV
Sbjct: 258 TYVSEEELVKNIQETPVPLSTALAISHSAFVKGDHTNFEIEPSIGVEASELYPNVHYTTV 317

Query: 301 EEYLDQFV 307
           E+YL+QFV
Sbjct: 318 EDYLNQFV 325

[Close]

BLAST of Cucsa.109540.1 vs. NCBI nr
Match: gi|700206685|gb|KGN61804.1| (hypothetical protein Csa_2G248135 [Cucumis sativus])

HSP 1 Score: 453.4 bits (1165), Expect = 3.2e-124
Identity = 220/308 (71.43%), Postives = 265/308 (86.04%), Query Frame = 1


Query: 1   MAQ--KVLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAKNRILNHFKSLGVNFL 60
           MAQ  KVL+IG TGYIGKF+V+ASA+AGHPT+AL+R+S+L   AK+ I+++F++LGVNF+
Sbjct: 1   MAQNLKVLVIGATGYIGKFVVEASAQAGHPTFALVRQSTLADSAKSSIIHNFRNLGVNFV 60

Query: 61  FGDLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHI 120
           FGD+FDNESLV+AI+QVDVVIST+G  ++ HQ KI+SAIKQAGNVKRF PSEFGND D +
Sbjct: 61  FGDIFDNESLVRAIQQVDVVISTVGRGLLSHQEKIISAIKQAGNVKRFLPSEFGNDVDRV 120

Query: 121 DAVEPAKSMYAAKAEFRRAIEAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILG 180
            AVEPAKSM+A+K E RRA+EAEGIPHTFVV NFFDGY+L N SQP A+ PPRDK+ I G
Sbjct: 121 HAVEPAKSMFASKVEIRRAVEAEGIPHTFVVSNFFDGYYLRNFSQPGATEPPRDKIKIFG 180

Query: 181 DGTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDLVSLWERKIGKSLKR 240
           DG  KVIYNKEED+GTYTIRAIDDPRT NKI+Y+RPPANIYSTN+LVSLWE+KIG+ L+R
Sbjct: 181 DGNLKVIYNKEEDIGTYTIRAIDDPRTFNKILYIRPPANIYSTNELVSLWEKKIGRILER 240

Query: 241 IYVPEEEVLKKIRETSYPLNIELALCHTAQVKGCQTNFSIEPSFGVEASALYPDVRYTTV 300
            YV EEE++K I+ET  PL+  LA+ H+A VKG  TNF IEPS GVEAS LYP+V YTTV
Sbjct: 241 TYVSEEELVKNIQETPVPLSTALAISHSAFVKGDHTNFEIEPSIGVEASELYPNVHYTTV 300

Query: 301 EEYLDQFV 307
           E+YL+QFV
Sbjct: 301 EDYLNQFV 308

[Close]

BLAST of Cucsa.109540.1 vs. NCBI nr
Match: gi|659122054|ref|XP_008460946.1| (PREDICTED: isoflavone reductase homolog [Cucumis melo])

HSP 1 Score: 451.1 bits (1159), Expect = 1.6e-123
Identity = 219/308 (71.10%), Postives = 261/308 (84.74%), Query Frame = 1


Query: 1   MAQK--VLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAKNRILNHFKSLGVNFL 60
           MAQK  VLIIGGTGYIGKF+V+ASA+AGHPT+ L+R+S+L   AK+ I++ F++LGVNF+
Sbjct: 1   MAQKLKVLIIGGTGYIGKFVVEASAQAGHPTFVLVRQSTLSDSAKSSIIDSFRNLGVNFV 60

Query: 61  FGDLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHI 120
           FGD+FDNESLV+AI+QVDVVIST+G  ++ HQ  I+SAIKQAGN+KRFFPSEFGND D +
Sbjct: 61  FGDIFDNESLVRAIQQVDVVISTVGRGLLSHQQNIISAIKQAGNIKRFFPSEFGNDVDRV 120

Query: 121 DAVEPAKSMYAAKAEFRRAIEAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILG 180
            AVEPAKSM+A K E RR +EAEGIPHTFVV NFFDGY+L N SQP A+ PPRDK+ I G
Sbjct: 121 HAVEPAKSMFARKVEIRRVVEAEGIPHTFVVSNFFDGYYLRNFSQPGATEPPRDKIKIFG 180

Query: 181 DGTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDLVSLWERKIGKSLKR 240
           DG  KVIYNKEED+GTYTIRAIDDPRT NKI+Y+RPPANIYSTN+LVSLWE+KI + L++
Sbjct: 181 DGNLKVIYNKEEDIGTYTIRAIDDPRTFNKILYIRPPANIYSTNELVSLWEKKICRILEK 240

Query: 241 IYVPEEEVLKKIRETSYPLNIELALCHTAQVKGCQTNFSIEPSFGVEASALYPDVRYTTV 300
            YV EEE++K I+ET  PLN  LA+ H+A VKG  TNF IEPS GVEAS LYPDV YTTV
Sbjct: 241 TYVSEEELVKNIQETPVPLNTALAISHSAFVKGDHTNFDIEPSIGVEASELYPDVHYTTV 300

Query: 301 EEYLDQFV 307
           E+YL+QFV
Sbjct: 301 EDYLNQFV 308

The following BLAST results are available for this feature:

BLAST of Cucsa.109540.1 vs. Swiss-Prot


Match Name	E-value	Identity	Description
ALL12_OLEEU	5.5e-116	66.34	Isoflavone reductase-like protein OS=Olea europaea PE=1 SV=1	[more]
IFRH_SOLTU	1.5e-113	64.03	Isoflavone reductase homolog OS=Solanum tuberosum PE=2 SV=1	[more]
IFRH_TOBAC	1.0e-106	59.74	Isoflavone reductase homolog A622 OS=Nicotiana tabacum PE=2 SV=1	[more]
IFRH_ARATH	5.4e-103	60.86	Isoflavone reductase homolog P3 OS=Arabidopsis thaliana GN=At1g75280 PE=2 SV=1	[more]
IFRH_MAIZE	1.9e-92	54.79	Isoflavone reductase homolog IRL OS=Zea mays GN=IRL PE=2 SV=1	[more]

BLAST of Cucsa.109540.1 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LJ01_CUCSA	1.7e-172	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G248130 PE=4 SV=1	[more]
A0A0A0LL65_CUCSA	2.2e-124	71.43	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G248135 PE=4 SV=1	[more]
M5VMM7_PRUPE	7.2e-123	70.30	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa008220mg PE=4 SV=1	[more]
V4SCG2_9ROSI	9.4e-123	69.64	Uncharacterized protein OS=Citrus clementina GN=CICLE_v10028904mg PE=4 SV=1	[more]
A9PF66_POPTR	1.2e-122	69.61	Putative uncharacterized protein OS=Populus trichocarpa PE=2 SV=1	[more]

BLAST of Cucsa.109540.1 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G39230.1	2.7e-121	67.66	NmrA-like negative transcriptional regulator family protein	[more]
AT1G75280.1	3.0e-104	60.86	NmrA-like negative transcriptional regulator family protein	[more]
AT1G75290.1	4.8e-102	58.55	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT1G75300.1	8.5e-99	56.60	NmrA-like negative transcriptional regulator family protein	[more]
AT1G19540.1	1.9e-90	54.19	NmrA-like negative transcriptional regulator family protein	[more]

BLAST of Cucsa.109540.1 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449462218\|ref\|XP_004148838.1\|	2.4e-172	100.00	PREDICTED: isoflavone reductase-like protein [Cucumis sativus]	[more]
gi\|659122052\|ref\|XP_008460945.1\|	3.0e-170	98.69	PREDICTED: isoflavone reductase-like protein [Cucumis melo]	[more]
gi\|778669354\|ref\|XP_004148840.2\|	3.2e-124	71.43	PREDICTED: isoflavone reductase-like protein [Cucumis sativus]	[more]
gi\|700206685\|gb\|KGN61804.1\|	3.2e-124	71.43	hypothetical protein Csa_2G248135 [Cucumis sativus]	[more]
gi\|659122054\|ref\|XP_008460946.1\|	1.6e-123	71.10	PREDICTED: isoflavone reductase homolog [Cucumis melo]	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **INTERPRO**
Term	Definition
IPR008030	NmrA-like
IPR016040	NAD(P)-bd_dom

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003674	molecular_function

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsa.109540	Cucsa.109540	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsa.109540.1	Cucsa.109540.1-protein	polypeptide

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsa.109540.1.CDS.5	Cucsa.109540.1.CDS.5	CDS
Cucsa.109540.1.CDS.4	Cucsa.109540.1.CDS.4	CDS
Cucsa.109540.1.CDS.3	Cucsa.109540.1.CDS.3	CDS
Cucsa.109540.1.CDS.2	Cucsa.109540.1.CDS.2	CDS
Cucsa.109540.1.CDS.1	Cucsa.109540.1.CDS.1	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsa.109540.1.three_prime_UTR.1	Cucsa.109540.1.three_prime_UTR.1	three_prime_UTR

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsa.109540.1.five_prime_UTR.1	Cucsa.109540.1.five_prime_UTR.1	five_prime_UTR

InterPro

Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008030	NmrA-like domain	PFAM	PF05368	NmrA	coord: 5..293 score: 4.6
IPR016040	NAD(P)-binding domain	GENE3D	G3DSA:3.40.50.720		coord: 3..96 score: 1.2
IPR016040	NAD(P)-binding domain	unknown	SSF51735	NAD(P)-binding Rossmann-fold domains	coord: 4..253 score: 2.5
None	No IPR available	GENE3D	G3DSA:3.90.25.10		coord: 150..306 score: 6.3
None	No IPR available	PANTHER	PTHR10366	NAD DEPENDENT EPIMERASE/DEHYDRATASE	coord: 266..306 score: 4.0E-157coord: 4..244 score: 4.0E
None	No IPR available	PANTHER	PTHR10366:SF375	SUBFAMILY NOT NAMED	coord: 266..306 score: 4.0E-157coord: 4..244 score: 4.0E