Cucsa.049600 (gene) Cucumber (Gy14) v1

BLAST of Cucsa.049600 vs. Swiss-Prot
Match: NDC80_CHICK (Kinetochore protein NDC80 homolog OS=Gallus gallus GN=NDC80 PE=1 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 3.6e-28
Identity = 124/579 (21.42%), Postives = 236/579 (40.76%), Query Frame = 1

Query: 29  VSFASSRPSSIGMSRPSSASIDMYKERAVQ----ISTVNTINSSLKELFS------HSFN 88
           VSF   R S  G SR S     M+    ++    +     I   +K+L        ++ N
Sbjct: 57  VSFFGKRASGAGGSRNSQYG--MFGTEKIKDPRPLHDKAFIQQCIKQLCEFLVENGYAHN 116

Query: 89  VTFKPFSPPSLKDLTETINLLLQCL----DYPPTKLEDDLPILLKSLGYPFKINKNILKN 148
           VT K    PS+KD  +    +   L    + P +K E+++P + K LGYPF ++K+ +  
Sbjct: 117 VTIKSLQSPSVKDFIKIFTFIYGFLCPSYELPDSKFEEEIPRVFKELGYPFPLSKSSMYT 176

Query: 149 PPAPHQWYQILALLHWLVQVALYNYNLANDSNPIFTGHDVN----------------IYV 208
             APH W QI+A L WL     +    A   NP       N                 YV
Sbjct: 177 VGAPHTWPQIVAALIWLTD--CFKLYAAMRENPSSFDDGQNWGGETDDGIVHNKLFMDYV 236

Query: 209 LNSYLHYMRGDDDSVEALDQEFMERLKKTRDDSRESLKELEEEVKVLEAKVEAL----RS 268
           +  Y H+M+G  D+ E LD E   +LK   +     ++ L  E K L  ++  L     S
Sbjct: 237 VKCYEHFMKG-GDTYEELDAEVQSKLKDLFNVDEFKIEGLAAENKRLHEEIARLEKERES 296

Query: 269 DPSQREVLEKQQSLLEEDVKKFHTMIAGYSEKMQEMKKLLEEKEKELDVKMEERNKICEE 328
           +P +   L K +S  + DV+K+   +A        + + ++   +E++    E   + +E
Sbjct: 297 EPDRLVSLRKLRSSFQADVQKYQAYLANLESHTTILDQKMKSVNEEVETTEMEVEAMKQE 356

Query: 329 NEELKKRVESQLFNPRDVERMRRELQAVERDIGDAEVSRNSWEEKSWDLDATLGNKFKEL 388
           N  L+   ++Q ++  D+ER+  E   +++ I        + E + W+ +       + +
Sbjct: 357 NARLQHIFDNQKYSVADIERINHERNELQQTINKLTKELEAEEHQLWNEELKYARNKEAI 416

Query: 389 EAIAMECNQAMRRLKL-------DSCHQYVLNAKGSTPADVMGIDYKTTLKPAL----DS 448
           E    E ++  R+LKL          H + ++       + + + Y+T +K  L    + 
Sbjct: 417 ETQLAEYHKLARKLKLIPISAENSKGHDFEIHFNPEAGPNCL-VKYRTQIKAPLMEIVNQ 476

Query: 449 FAEDIRRSSMMKLEELISLRQQSSENTAKIESKRDHIASLQSHVNEHETQLISLKDEMRD 508
             E+IR+++  K+    SL     +  A +  K+  + +L+    +       L D    
Sbjct: 477 TEEEIRKATQRKM----SLEDTLEQVNAMVAEKKSSVKTLKEEAEK-------LDDLYHQ 536

Query: 509 YILRCSTESKKMVEDVQQEAHNLDLVEGEAEESLKNAKLAHQEAIKQGEEEIHMCARELL 563
            +     E +K   +++    +  L+E    E L  A     +  +Q +  +     E  
Sbjct: 537 KLKEAEEEEQKCANELELLEKHKQLLESGVNEGLSEATNELHDIQRQYQIVMQTTTEESR 596

BLAST of Cucsa.049600 vs. Swiss-Prot
Match: NDC80_MACFA (Kinetochore protein NDC80 homolog OS=Macaca fascicularis GN=NDC80 PE=2 SV=1)

HSP 1 Score: 122.1 bits (305), Expect = 2.0e-26
Identity = 112/495 (22.63%), Postives = 217/495 (43.84%), Query Frame = 1

Query: 75  HSFNVTFKPFSPPSLKDLTETINLLLQCL----DYPPTKLEDDLPILLKSLGYPFKINKN 134
           ++ NV+ K    PS+KD  +    L   L    + P TK E+++P + K LGYPF ++K+
Sbjct: 108 YAHNVSMKSLQAPSVKDFLKIFTFLYGFLCPSYELPDTKFEEEVPRIFKDLGYPFALSKS 167

Query: 135 ILKNPPAPHQWYQILALLHWLVQVALYNYNLANDSNPIFT-----GHDVN---------- 194
            +    APH W  I+A L WL+   +  +    +S+P+F      G +            
Sbjct: 168 SMYTVGAPHTWPHIVAALVWLID-CIKIHTAMKESSPLFDDGQPWGEETEDGIMHNKLFL 227

Query: 195 IYVLNSYLHYMRGDDDSVEALDQEFMERLKKTRDDSRESLKELEEEVKVLEAKVEAL--- 254
            Y +  Y  +M G  DS + ++ E   +LK   +     L+ LE + + L  ++  L   
Sbjct: 228 DYTIKCYESFMSG-ADSFDEMNAELQSKLKDLFNVDAFKLESLEAKNRALNEQIARLEQE 287

Query: 255 -RSDPSQREVLEKQQSLLEEDVKKFHTMIAGYSEKMQEMKKLLEEKEKELDVKMEERNKI 314
              +P++ E L K ++ L+ DV+K+      Y   ++    +L++K   L+ ++      
Sbjct: 288 REKEPNRLESLRKLKASLQGDVQKYQ----AYMSNLESHSAILDQKLNGLNEEIARVELE 347

Query: 315 CE----ENEELKKRVESQLFNPRDVERMRRELQAVERDIGDAEVSRNSWEEKSWDLDATL 374
           CE    EN  L+  +++Q ++  D+ER+  E   +++ I        + ++K W+ +   
Sbjct: 348 CETIKQENTRLQNIIDNQKYSVADIERINHERNELQQTINKLTKDLEAEQQKLWNEELKY 407

Query: 375 GNKFKELEAIAMECNQAMRRLKLDSCHQYVLNAKGSTPADVMGIDYKTTLKP-ALDSFAE 434
               + +E    E ++  R+LK        L  KG+   +  G D++    P A  +   
Sbjct: 408 ARGKEAIETQLAEYHKLARKLK--------LIPKGA--ENSKGYDFEIKFNPEAGANCLV 467

Query: 435 DIRRSSMMKLEELISLRQQSSENTAKIESKRDHIASLQSHVNEHETQLISLKDEMRDYIL 494
             R    + L+EL++                      +  +N+   + + L+D + + + 
Sbjct: 468 KYRAQVYVPLKELLN--------------------ETEEEINKARNKKMGLEDTL-EQLN 527

Query: 495 RCSTESKKMVEDVQQEAHNLDLVEGEAEESLKNAKLAHQEAIKQGEEEIHMCARELLSCL 542
              TESK+ V  +++E   LD +              +Q+ IK+ EEE   CA E    L
Sbjct: 528 AMITESKRSVRTLKEEVQKLDDL--------------YQQKIKEAEEEDEKCASE----L 547

BLAST of Cucsa.049600 vs. Swiss-Prot
Match: NDC80_HUMAN (Kinetochore protein NDC80 homolog OS=Homo sapiens GN=NDC80 PE=1 SV=1)

HSP 1 Score: 119.0 bits (297), Expect = 1.7e-25
Identity = 111/527 (21.06%), Postives = 227/527 (43.07%), Query Frame = 1

Query: 75  HSFNVTFKPFSPPSLKDLTETINLLLQCL----DYPPTKLEDDLPILLKSLGYPFKINKN 134
           ++ NV+ K    PS+KD  +    L   L    + P TK E+++P + K LGYPF ++K+
Sbjct: 108 YAHNVSMKSLQAPSVKDFLKIFTFLYGFLCPSYELPDTKFEEEVPRIFKDLGYPFALSKS 167

Query: 135 ILKNPPAPHQWYQILALLHWLVQVALYNYNLANDSNPIFT-----GHDVN---------- 194
            +    APH W  I+A L WL+   +  +    +S+P+F      G +            
Sbjct: 168 SMYTVGAPHTWPHIVAALVWLID-CIKIHTAMKESSPLFDDGQPWGEETEDGIMHNKLFL 227

Query: 195 IYVLNSYLHYMRGDDDSVEALDQEFMERLKKTRDDSRESLKELEEEVKVLEAKVEAL--- 254
            Y +  Y  +M G  DS + ++ E   +LK   +     L+ LE + + L  ++  L   
Sbjct: 228 DYTIKCYESFMSG-ADSFDEMNAELQSKLKDLFNVDAFKLESLEAKNRALNEQIARLEQE 287

Query: 255 -RSDPSQREVLEKQQSLLEEDVKKFHTMIAGYSEKMQEMKKLLEEKEKELDVKMEERNKI 314
              +P++ E L K ++ L+ DV+K+      Y   ++    +L++K   L+ ++      
Sbjct: 288 REKEPNRLESLRKLKASLQGDVQKYQ----AYMSNLESHSAILDQKLNGLNEEIARVELE 347

Query: 315 CE----ENEELKKRVESQLFNPRDVERMRRELQAVERDIGDAEVSRNSWEEKSWDLDATL 374
           CE    EN  L+  +++Q ++  D+ER+  E   +++ I        + ++K W+ +   
Sbjct: 348 CETIKQENTRLQNIIDNQKYSVADIERINHERNELQQTINKLTKDLEAEQQKLWNEELKY 407

Query: 375 GNKFKELEAIAMECNQAMRRLKLDSCHQYVLNAKGSTPADVMGIDYKTTLKP-ALDSFAE 434
               + +E    E ++  R+LK        L  KG+   +  G D++    P A  +   
Sbjct: 408 ARGKEAIETQLAEYHKLARKLK--------LIPKGA--ENSKGYDFEIKFNPEAGANCLV 467

Query: 435 DIRRSSMMKLEELISLRQQSSENTAKIESKR----DHIASLQSHVNEHETQLISLKDEM- 494
             R    + L+EL++   ++ E   K  +K+    D +  L + + E +  + +LK+E+ 
Sbjct: 468 KYRAQVYVPLKELLN---ETEEEINKALNKKMGLEDTLEQLNAMITESKRSVRTLKEEVQ 527

Query: 495 ------RDYILRCSTESKKMVEDVQQEAHNLDLVEGEAEESLKNAKLAHQEAIKQGEEEI 554
                 +  I     E +K   +++    +  L+E    + L  A        ++ +  +
Sbjct: 528 KLDDLYQQKIKEAEEEDEKCASELESLEKHKHLLESTVNQGLSEAMNELDAVQREYQLVV 587

Query: 555 HMCARELLSCLDSVSQYKAVVSCKISDMESCLSKAAAGISEAFKNSM 563
                E     +++ +   +V+  +  +E  L +  A +   ++  M
Sbjct: 588 QTTTEERRKVGNNLQRLLEMVATHVGSVEKHLEEQIAKVDREYEECM 615

BLAST of Cucsa.049600 vs. Swiss-Prot
Match: NDC80_XENTR (Kinetochore protein NDC80 homolog OS=Xenopus tropicalis GN=ndc80 PE=2 SV=1)

HSP 1 Score: 115.9 bits (289), Expect = 1.4e-24
Identity = 109/502 (21.71%), Postives = 218/502 (43.43%), Query Frame = 1

Query: 75  HSFNVTFKPFSPPSLKDLTETINLLLQ--CLDY--PPTKLEDDLPILLKSLGYPFKINKN 134
           +S  +T K    PS KD  +    +    C +Y  P +K E+++P + K LGYPF ++K+
Sbjct: 107 YSQTLTVKSLQGPSTKDFLKIFAFIYTFICPNYENPESKFEEEIPRIFKELGYPFALSKS 166

Query: 135 ILKNPPAPHQWYQILALLHWLVQVALYNYNLANDSNPIF----------TGHDVN----I 194
            +    APH W QI+A L WL+        L ++ NP+F           G D N     
Sbjct: 167 SMYTVGAPHTWPQIVAALVWLIDCVKLCCVLRSE-NPVFEEPQMGEQSENGIDFNQLFLD 226

Query: 195 YVLNSYLHYMRGDDDSVEALDQEFMERLKKTRDDSRESLKELEEEVKVLEAKVEAL---- 254
           Y +  Y  +M G  D+ E  D +   RLK+       +L+ L++E + L  ++E L    
Sbjct: 227 YTVRCYDQFMEG-RDTFEEYDADMCIRLKEAYHVDESNLEALQQESRRLLEEIERLEKEK 286

Query: 255 RSDPSQREVLEKQQSLLEEDVKKFHTMIAGYSEKMQEMKKLLEEKEKELDVKMEERNKIC 314
            ++P +   + K +  L+ D++K+   +         +++ +    ++L+    E   + 
Sbjct: 287 ENEPDRLASMRKLKVSLQADIQKYQNYLTEIESHSTLLEQRVSSVSEDLEATELESRAVQ 346

Query: 315 EENEELKKRVESQLFNPRDVERMRRELQAVERDIGDAEVSRNSWEEKSWDLDATLGNKFK 374
           +EN  LK  +++Q ++  D+ER++ E   +++ I       +  +++ W  +       +
Sbjct: 347 QENLRLKNILDNQKYSVADIERIKYEENELQQTIAKLTKDLDEDKQQLWSEELKYAKMKE 406

Query: 375 ELEAIAMECNQAMRRLKLDSCHQYVLNAKGSTPADVMGIDYKTTLKPALDSFAEDI---R 434
            +E    E ++  R+++L              P       Y   ++  LDS    +   R
Sbjct: 407 SVETQLSEFHKIGRKVRL------------IPPTAEYANGYDFQIQCNLDSEQSSLIHYR 466

Query: 435 RSSMMKLEELISLRQ-QSSENTAKIESKRDHIASLQSHVNEHETQLISLKDE-------M 494
               + L E++S  + Q +  T K     D +  L + + E + ++   KDE        
Sbjct: 467 NKINVPLVEILSQSEGQIAGATNKKMGVEDMVEQLNTLIGEKKNEVKVQKDEAQKLEETY 526

Query: 495 RDYILRCSTESKKMVEDVQQEAHNLDLVEG----EAEESLKNAKLAHQE---AIKQGEEE 537
           +  +     + K+ + +++    +  L+E       +E+LK+ +   QE      Q EEE
Sbjct: 527 QQKVEEAEEDEKRWISEIESLEKHRQLLESGVNKSLDEALKDLQKEQQELQLVEHQTEEE 586

BLAST of Cucsa.049600 vs. Swiss-Prot
Match: NDC80_DANRE (Kinetochore protein NDC80 homolog OS=Danio rerio GN=ndc80 PE=2 SV=1)

HSP 1 Score: 115.5 bits (288), Expect = 1.8e-24
Identity = 111/475 (23.37%), Postives = 208/475 (43.79%), Query Frame = 1

Query: 78  NVTFKPFSPPSLKDLTETINLLLQCLD----YPPTKLEDDLPILLKSLGYPFKINKNILK 137
           ++T K    PS K+  +    +   L+     P  K+E+++P +LK LGYPF ++K+ + 
Sbjct: 109 SITVKALQSPSTKEFLKIYEFIYNFLEPSFQMPTAKVEEEIPRMLKDLGYPFALSKSSMY 168

Query: 138 NPPAPHQWYQILALLHWLVQ-VALYNYNLAND------SNPIFTGHD-------VNIYVL 197
           +  APH W   L  L WL+  V L+      D      S+ +    D       +  Y  
Sbjct: 169 SIGAPHTWPLALGALIWLMDAVKLFGGQREQDLLFSDFSDELCDLEDRTEYNKLIMEYCS 228

Query: 198 NSYLHYMRGDDDSVEALDQEFMERLKKTRDDSRESLKELEEEVKVLEAKVEAL-RSDPSQ 257
           ++Y  +M+G  D+ +  D +F+ +LKK  +     L   +E+  +L   VE L R   + 
Sbjct: 229 DTYNKFMQG-ADTFDDEDDDFLYKLKKLYNVDEALLHSQQEKHSMLMEHVERLERESQTD 288

Query: 258 REVLEKQQSL-LEEDVKKFHTMIAGYSEKMQEMKKLLEEKE----KELDVKMEERNKICE 317
           R V ++ + L L+ D++K    +  Y   ++  K  LE K     +EL+    +   + +
Sbjct: 289 RLVGKRTEKLRLQTDLQK----LQNYRCTLEAHKTGLENKSAGLTEELEAVEMQLEGLKQ 348

Query: 318 ENEELKKRVESQLFNPRDVERMRRELQAVERDIGDAEVSRNSWEEKSWDLDATLGNKFKE 377
           E   L+  +E+Q F P D+ER+ RE   +++ I     S    E+  W+ +  L    ++
Sbjct: 349 ERTRLQHILENQKFTPADIERINRERNELQQTIHGLSQSLEEGEQLVWNEEVNLSKTKEK 408

Query: 378 LEAIAMECNQAMRRLKL------DSC-HQYVLNAKGSTPADVMGIDYKTTLKPALDSFAE 437
            E    E N+  R+LKL      ++C H + + A  S         YKT ++  L +   
Sbjct: 409 AELKVAEYNKLGRKLKLIPLSAENACGHDFEIRADYSATTIT---QYKTQIQNPLKNMMV 468

Query: 438 DIRRSSMMKLEELISLRQQSSENTAKIESKRDHIASLQSHVNEHETQLISLKDEMRDYIL 497
           ++           +SL +   +  + I  K + I  L+  + + + QL +   EM     
Sbjct: 469 EVEEEFSRLSNVNLSLEETVEQVKSNIFDKENDIKQLKEQIRKVDQQLENAMQEM----- 528

Query: 498 RCSTESKKMVEDVQQEAHNLDLVEGEAEESLKNAKLAHQEAIKQGEEEIHMCARE 522
             + E  K   ++     +  L E    + ++ A    +E +K  +++ H+  +E
Sbjct: 529 --ALEDDKWAAELDSAETHKKLFEKNVMQGIEEA----EEEVKAAQQQYHVVVQE 564

BLAST of Cucsa.049600 vs. TrEMBL
Match: F6I2P0_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_16s0013g00940 PE=4 SV=1)

HSP 1 Score: 642.5 bits (1656), Expect = 4.8e-181
Identity = 334/571 (58.49%), Postives = 432/571 (75.66%), Query Frame = 1

Query: 1   MKGTARRRPKASFNPPPPPTP------QFRRDSDVSFASSRPSSIGMSRPSSASIDMYKE 60
           MK T RRRPK SF PPPPP P      Q  RDSD SFA SRPSS+G+SR S+   D   +
Sbjct: 215 MKATGRRRPKDSFRPPPPPPPSVDHHRQLSRDSDASFAGSRPSSLGISRSSAG--DALTD 274

Query: 61  RAVQISTVNTINSSLKELFSHSFNVTFKPFSPPSLKDLTETINLLLQCLDYPPTKLEDDL 120
           R+ Q+S + TINS L    SHS  ++ KP   PS KD++ET+  L+  LD+P TKLEDDL
Sbjct: 275 RSYQLSAIRTINSFLA---SHSSPLSLKP-PLPSAKDISETLKFLISQLDFPTTKLEDDL 334

Query: 121 PILLKSLGYPFKINKNILKNPPAPHQWYQILALLHWLVQVALYNYNLANDSNPIFTGHDV 180
           PILLK L  P K+NK+ LK P  PH W  +L ++HWLVQ+ALYN +L ++        + 
Sbjct: 335 PILLKHLNCPIKLNKSALKAPGTPHAWPSLLGVMHWLVQIALYNDHLVSNLQCF---DNT 394

Query: 181 NIYVLNSYLHYMRGDDDSVEALDQEFMERLKKTRDDSRESLKELEEEVKVLEAKVEALRS 240
            +Y L+SYLHY+RGDDD +EA+D+EFM +L++ RD   E +K LE+EV   E ++E LR 
Sbjct: 395 FVYALDSYLHYIRGDDDGMEAVDREFMGKLERERDAVAEGVKALEKEVAEREGRLEELRL 454

Query: 241 DPSQREVLEKQQSLLEEDVKKFHTMIAGYSEKMQEMKKLLEEKEKELDVKMEERNKICEE 300
            PS +EV+EK++ +LEEDVKKFH +IA +S ++  ++K+LEEKEKEL VK+EE N+ICEE
Sbjct: 455 GPSAKEVVEKERGVLEEDVKKFHAIIAEFSGRIASVEKILEEKEKELGVKVEENNRICEE 514

Query: 301 NEELKKRVESQLFNPRDVERMRRELQAVERDIGDAEVSRNSWEEKSWDLDATLGNKFKEL 360
           NEELKKRVE Q FN RD ERM+RELQAVERDI +AEV+RN WEEKSWDLD T+G+KFKEL
Sbjct: 515 NEELKKRVELQTFNARDAERMKRELQAVERDITEAEVARNGWEEKSWDLDTTIGHKFKEL 574

Query: 361 EAIAMECNQAMRRLKLDSCHQYVLNAKGSTPADVMGIDYKTTLKPALDSFAEDIRRSSMM 420
           EA+++ECNQA+RRLKL +  QYVLNAKGS+PA+V+GIDYK+TLKPALDSFA+DI +SSM 
Sbjct: 575 EALSIECNQALRRLKLGNGLQYVLNAKGSSPAEVLGIDYKSTLKPALDSFADDINKSSMS 634

Query: 421 KLEELISLRQQSSENTAKIESKRDHIASLQSHVNEHETQLISLKDEMRDYILRCSTESKK 480
           KLEELISL+QQS EN AKIE+KR+ +A+LQS  +E E QLI LK E ++Y  RC+TE+KK
Sbjct: 635 KLEELISLQQQSVENAAKIEAKRNRLAALQSSSDEVEAQLIFLKKESQNYTSRCATEAKK 694

Query: 481 MVEDVQQEAHNLDLVEGEAEESLKNAKLAHQEAIKQGEEEIHMCARELLSCLDSVSQYKA 540
           +VEDV+ E HN+D+VE E  + L+ +KL  QEAIKQ EEEI MCA EL + +DSVS+YK 
Sbjct: 695 LVEDVEIETHNVDIVEREVADVLETSKLKLQEAIKQNEEEIQMCAWELYALVDSVSKYKE 754

Query: 541 VVSCKISDMESCLSKAAAGISEAFKNSMPAE 566
            ++ KIS+M+  LS+ A  +S+++K S+ A+
Sbjct: 755 HMASKISEMKCNLSETAGAVSDSYKGSLLAQ 776

BLAST of Cucsa.049600 vs. TrEMBL
Match: F6H969_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_12s0034g00950 PE=4 SV=1)

HSP 1 Score: 637.9 bits (1644), Expect = 1.2e-179
Identity = 332/571 (58.14%), Postives = 430/571 (75.31%), Query Frame = 1

Query: 1   MKGTARRRPKASFNPPPPPTP------QFRRDSDVSFASSRPSSIGMSRPSSASIDMYKE 60
           MK T RRRPK S  PPPPP P      QF RDSD SFASSRPSS+G++R S+   D   +
Sbjct: 1   MKATGRRRPKDSIRPPPPPAPSVDHHRQFSRDSDASFASSRPSSLGINRSSAG--DALTD 60

Query: 61  RAVQISTVNTINSSLKELFSHSFNVTFKPFSPPSLKDLTETINLLLQCLDYPPTKLEDDL 120
           R+ Q+S + TINS L    SHS  ++ KP   PS KD++ET+  L+  LD+P TKLEDDL
Sbjct: 61  RSYQLSAIRTINSFLA---SHSSPLSLKP-PLPSAKDISETLKFLISQLDFPTTKLEDDL 120

Query: 121 PILLKSLGYPFKINKNILKNPPAPHQWYQILALLHWLVQVALYNYNLANDSNPIFTGHDV 180
           PILLK L  P K+NK+ LK P  PH W  +L ++HWLVQ+ALYN +L ++        + 
Sbjct: 121 PILLKHLNCPIKLNKSALKAPGTPHAWPPLLGVMHWLVQIALYNDHLVSNLQCF---DNT 180

Query: 181 NIYVLNSYLHYMRGDDDSVEALDQEFMERLKKTRDDSRESLKELEEEVKVLEAKVEALRS 240
            +Y L+SYLHY+RGDDD +EA+D+EFM +L+K RD   E +K LE+EV   E ++E LRS
Sbjct: 181 FVYALDSYLHYIRGDDDGMEAVDREFMGKLEKERDAVAEGVKALEKEVAEREGRLEELRS 240

Query: 241 DPSQREVLEKQQSLLEEDVKKFHTMIAGYSEKMQEMKKLLEEKEKELDVKMEERNKICEE 300
            PS +EV+EK + +LEEDVKKFH +IA +S ++  ++K+LEEKEKEL VK+EE N+ICEE
Sbjct: 241 GPSAKEVVEKVRGVLEEDVKKFHAIIAEFSGRIASVEKILEEKEKELGVKVEENNRICEE 300

Query: 301 NEELKKRVESQLFNPRDVERMRRELQAVERDIGDAEVSRNSWEEKSWDLDATLGNKFKEL 360
           NEELKKRVE Q FN RD ERM+RELQAVERDI +AEV+RN WEEKSWDLD T+G+KFKEL
Sbjct: 301 NEELKKRVELQTFNARDAERMKRELQAVERDITEAEVARNGWEEKSWDLDTTIGHKFKEL 360

Query: 361 EAIAMECNQAMRRLKLDSCHQYVLNAKGSTPADVMGIDYKTTLKPALDSFAEDIRRSSMM 420
           EA+++ECNQA+RRLKL +  QYVLNAKGS+P +V+GIDYK+ LKPALDSFA+DI +SSM 
Sbjct: 361 EALSIECNQALRRLKLGNGLQYVLNAKGSSPTEVLGIDYKSALKPALDSFADDINKSSMS 420

Query: 421 KLEELISLRQQSSENTAKIESKRDHIASLQSHVNEHETQLISLKDEMRDYILRCSTESKK 480
           KLEELISL+QQS EN AKIE+KR+ +A+LQS  +E E QL  LK E ++Y  RC+ E+KK
Sbjct: 421 KLEELISLQQQSVENAAKIEAKRNRLAALQSRSDEVEAQLNFLKKETQNYTSRCAMEAKK 480

Query: 481 MVEDVQQEAHNLDLVEGEAEESLKNAKLAHQEAIKQGEEEIHMCARELLSCLDSVSQYKA 540
           +VEDV+ E HN+D+VE E  + L+ +KL  QEAIKQ EEEI +CA EL + +DSVS+YK 
Sbjct: 481 LVEDVEIETHNVDIVEREVADVLETSKLKLQEAIKQNEEEIQICAWELYALVDSVSKYKE 540

Query: 541 VVSCKISDMESCLSKAAAGISEAFKNSMPAE 566
            ++ KIS+M+S LS+ A  +S+++K S+ A+
Sbjct: 541 HMASKISEMKSNLSETAGAVSDSYKGSLSAQ 562

BLAST of Cucsa.049600 vs. TrEMBL
Match: A5AI77_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_035303 PE=4 SV=1)

HSP 1 Score: 636.3 bits (1640), Expect = 3.5e-179
Identity = 332/571 (58.14%), Postives = 429/571 (75.13%), Query Frame = 1

Query: 1   MKGTARRRPKASFNPPPPPTP------QFRRDSDVSFASSRPSSIGMSRPSSASIDMYKE 60
           MK T RRRPK S  PPPPP P      QF RDSD SFASSRPSS+G++R S+   D   +
Sbjct: 1   MKATGRRRPKDSIRPPPPPAPSVDHHRQFSRDSDASFASSRPSSLGINRSSAG--DALTD 60

Query: 61  RAVQISTVNTINSSLKELFSHSFNVTFKPFSPPSLKDLTETINLLLQCLDYPPTKLEDDL 120
           R+ Q+S + TINS L    SHS  ++ KP   PS KD++ET+  L+  LD+P TKLEDDL
Sbjct: 61  RSYQLSAIRTINSFLA---SHSSPLSLKP-PLPSAKDISETLKFLISQLDFPTTKLEDDL 120

Query: 121 PILLKSLGYPFKINKNILKNPPAPHQWYQILALLHWLVQVALYNYNLANDSNPIFTGHDV 180
           PILLK L  P K+NK+ LK P  PH W  +L ++HWLVQ+ALYN +L ++        + 
Sbjct: 121 PILLKHLNCPIKLNKSALKAPGTPHAWPPLLGVMHWLVQIALYNDHLVSNLQCF---DNT 180

Query: 181 NIYVLNSYLHYMRGDDDSVEALDQEFMERLKKTRDDSRESLKELEEEVKVLEAKVEALRS 240
            +Y L+SYLHY+RGDDD +EA+D+EFM +L+  RD   E +K LE+EV   E ++E LRS
Sbjct: 181 FVYALDSYLHYIRGDDDGMEAVDREFMGKLEXERDAVAEGVKALEKEVAEREGRLEELRS 240

Query: 241 DPSQREVLEKQQSLLEEDVKKFHTMIAGYSEKMQEMKKLLEEKEKELDVKMEERNKICEE 300
            PS +EV+EK++ +LEEDVKKFH +IA +S ++  ++K+LEEKEKEL VK+EE N+ICEE
Sbjct: 241 GPSAKEVVEKERGVLEEDVKKFHAIIAEFSGRIASVEKILEEKEKELGVKVEENNRICEE 300

Query: 301 NEELKKRVESQLFNPRDVERMRRELQAVERDIGDAEVSRNSWEEKSWDLDATLGNKFKEL 360
           NEELKKRVE Q FN RD ERM+RELQAVERDI +AEV+RN WEEKSWDLD T+G+KFKEL
Sbjct: 301 NEELKKRVELQTFNARDAERMKRELQAVERDITEAEVARNGWEEKSWDLDTTIGHKFKEL 360

Query: 361 EAIAMECNQAMRRLKLDSCHQYVLNAKGSTPADVMGIDYKTTLKPALDSFAEDIRRSSMM 420
           EA+++ECNQA+RRLKL +  QYVLNAKGS+P +V+GIDYK+ LKPALDSFA+DI +SSM 
Sbjct: 361 EALSIECNQALRRLKLGNGLQYVLNAKGSSPXEVLGIDYKSXLKPALDSFADDINKSSMS 420

Query: 421 KLEELISLRQQSSENTAKIESKRDHIASLQSHVNEHETQLISLKDEMRDYILRCSTESKK 480
           KLEELISL+QQS EN AKIE+KR+ +A+LQS  +E E QLI LK E ++Y  RC+TE+KK
Sbjct: 421 KLEELISLQQQSVENAAKIEAKRNRLAALQSXSDEVEAQLIFLKKESQNYTSRCATEAKK 480

Query: 481 MVEDVQQEAHNLDLVEGEAEESLKNAKLAHQEAIKQGEEEIHMCARELLSCLDSVSQYKA 540
           +VEDV+ E HN+D VE E  + L+ +KL  QEAIKQ EEEI  CA EL + +DSVS+YK 
Sbjct: 481 LVEDVEIETHNVDXVEREVADVLETSKLKLQEAIKQNEEEIQXCAWELYALVDSVSKYKE 540

Query: 541 VVSCKISDMESCLSKAAAGISEAFKNSMPAE 566
            ++ KIS+M+  LS+ A  +S+++K S+ A+
Sbjct: 541 HMASKISEMKCNLSETAGAVSDSYKGSLLAQ 562

BLAST of Cucsa.049600 vs. TrEMBL
Match: M5XWK4_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa003403mg PE=4 SV=1)

HSP 1 Score: 612.1 bits (1577), Expect = 7.0e-172
Identity = 318/564 (56.38%), Postives = 426/564 (75.53%), Query Frame = 1

Query: 7   RRPKASFNP-PPPPTP--QFR-RDSDVSFASSRPSSIGMSRPSSASIDMYKERAVQISTV 66
           RRPK SFNP PPP TP   FR RDSD SFASSRPSS+GM  P+S ++D+YK+R+ Q +T+
Sbjct: 9   RRPKESFNPRPPPATPLDHFRHRDSDASFASSRPSSVGMG-PTSNALDLYKDRSFQQATL 68

Query: 67  NTINSSLKELFSHSFNVTFKPFSPPSLKDLTETINLLLQCLDYPPTKLEDDLPILLKSLG 126
           ++INS L    SHS  +  K F  PS K++TET+  L+  LDYP  KLEDDLP++LK L 
Sbjct: 69  SSINSYLA---SHSLQIFLK-FPIPSAKEITETLKFLVARLDYPNPKLEDDLPVILKFLK 128

Query: 127 YPFKINKNILKNPPAP-HQWYQILALLHWLVQVALYNYNLANDSNPIFTGHDVNIYVLNS 186
            PFK NK++L+NP    HQW  +LA++HWL Q+ LYN +LA++S      + ++ Y L S
Sbjct: 129 CPFKPNKSVLRNPTVNHHQWPTLLAVIHWLFQIVLYNDHLASNSGAFVDNNAMSAYALES 188

Query: 187 YLHYMRGDDDSVEALDQEFMERLKKTRDDSRESLKELEEEVKVLEAKVEALRSDPSQREV 246
           Y +Y+ GDDDSVEALD++F+E+L+  R+++ ES++ LE     LE K+E +RS PS+RE 
Sbjct: 189 YSYYISGDDDSVEALDRQFLEKLEAERENAEESIRVLEATAAELEGKMEEMRSGPSKREA 248

Query: 247 LEKQQSLLEEDVKKFHTMIAGYSEKMQEMKKLLEEKEKELDVKMEERNKICEENEELKKR 306
           LEK++ +LE+DV KF+ MI   ++ + +++ +LE KEKEL+ K+ +  +ICEENEELKKR
Sbjct: 249 LEKERGVLEQDVNKFNEMIGSLAKSVAKLEAVLEAKEKELEAKVADTRRICEENEELKKR 308

Query: 307 VESQLFNPRDVERMRRELQAVERDIGDAEVSRNSWEEKSWDLDATLGNKFKELEAIAMEC 366
           VE Q FN RDV+RM+RELQAVERDIG+AE++RN+WEEK+WDLD  L +KFKELE +AMEC
Sbjct: 309 VELQTFNARDVDRMKRELQAVERDIGEAELARNAWEEKAWDLDTMLSHKFKELETLAMEC 368

Query: 367 NQAMRRLKLDSCHQYVLNAKGSTPADVMGIDYKTTLKPALDSFAEDIRRSSMMKLEELIS 426
           NQAMRRLKL +  QYVLNAKGST ADVMGIDYK TLKPAL+S+++DI++SS+ KLEELIS
Sbjct: 369 NQAMRRLKLGNGFQYVLNAKGSTAADVMGIDYKLTLKPALNSYSDDIKKSSVEKLEELIS 428

Query: 427 LRQQSSENTAKIESKRDHIASLQSHVNEHETQLISLKDEMRDYILRCSTESKKMVEDVQQ 486
           L+QQSSE +A IE KR+ IA+LQSH++E E QL  LK E  DY  RC+ E+++M++DVQ 
Sbjct: 429 LQQQSSELSATIEGKRNFIATLQSHIDEVEAQLNLLKKETHDYTYRCAIEARQMMDDVQM 488

Query: 487 EAHNLDLVEGEAEESLKNAKLAHQEAIKQGEEEIHMCARELLSCLDSVSQYKAVVSCKIS 546
           EAHNLD++E +AEE LK +KL  QE IKQ EEE   CA EL++ +DSVS++K  V  KI 
Sbjct: 489 EAHNLDILERDAEEILKTSKLKLQETIKQTEEETKKCAYELMTVIDSVSKHKEYVQSKIL 548

Query: 547 DMESCLSKAAAGISEAFKNSMPAE 566
           +M   +S+ A  +S+A K S+ ++
Sbjct: 549 EMRRDVSETAVAVSDAHKGSLQSQ 567

BLAST of Cucsa.049600 vs. TrEMBL
Match: A0A061DY97_THECC (Kinetochore protein ndc80, putative OS=Theobroma cacao GN=TCM_006424 PE=4 SV=1)

HSP 1 Score: 597.8 bits (1540), Expect = 1.4e-167
Identity = 316/567 (55.73%), Postives = 412/567 (72.66%), Query Frame = 1

Query: 1   MKGTARRRPKASFNPPPPPTPQFRRDSDVSFASSRPSSIGMSRPSSASIDMYKERAVQIS 60
           M+GT RRRP  SFNPP    PQ  RDSD SFASSRPSSIGM R  SA+ D Y +RA Q +
Sbjct: 1   MRGTTRRRPTDSFNPP---VPQPSRDSDASFASSRPSSIGMGRAISAA-DPYSDRAFQTA 60

Query: 61  TVNTINSSLKELFSHSFN-VTFKPFSPPSLKDLTETINLLLQCLDYPPTKLEDDLPILLK 120
           T+ +IN+      SHS   ++ KP   PS KDL+  +  LL  L +P +KLE+DL  LLK
Sbjct: 61  TIRSINAFFS---SHSIPPISTKPSQAPSAKDLSNILTSLLSLLHFPCSKLEEDLGPLLK 120

Query: 121 SLGYPFKINKNILKNPPAPHQWYQILALLHWLVQVALYNYNLANDSNPIFTGHD-VNIYV 180
           SL  PFK NK+ L+ P  PH W   L ++HWLVQ+A+YN +L+ +S   F   D +  Y 
Sbjct: 121 SLNCPFKFNKSTLRAPNTPHNWPNWLGIIHWLVQLAMYNEHLSQNSTTSFAQSDSMTEYA 180

Query: 181 LNSYLHYMRGDDDSVEALDQEFMERLKKTRDDSRESLKELEEEVKVLEAKVEALRSDPSQ 240
           L SY+ ++RG+DD +E LD+EFME+L+K R++  E+ + LE+ V  LE K E LR+ P++
Sbjct: 181 LESYMRFIRGEDDLLEVLDKEFMEKLEKERENVVENSRALEKNVGELEVKAEGLRTGPTE 240

Query: 241 REVLEKQQSLLEEDVKKFHTMIAGYSEKMQEMKKLLEEKEKELDVKMEERNKICEENEEL 300
           R+VLEK++++LEEDVKKFH MIA ++ ++  M+K+LEEKEKEL+ K EER ++CEENEEL
Sbjct: 241 RQVLEKEKNVLEEDVKKFHAMIAEFTGRIGAMEKVLEEKEKELNAKEEERRRVCEENEEL 300

Query: 301 KKRVESQLFNPRDVERMRRELQAVERDIGDAEVSRNSWEEKSWDLDATLGNKFKELEAIA 360
           KKRVE Q FN RDVERM+RE+QAVERDIG+AEV+RNSWE+KSWDLD+T+G KFKEL A+ 
Sbjct: 301 KKRVELQTFNARDVERMKREMQAVERDIGEAEVARNSWEDKSWDLDSTIGQKFKELMALT 360

Query: 361 MECNQAMRRLKLDSCHQYVLNAKGSTPADVMGIDYKTTLKPALDSFAEDIRRSSMMKLEE 420
           MECNQA+RRLKL    QY LNAKGSTPA+VMGIDYK TLKP L+S+A+ IR SS  K E+
Sbjct: 361 MECNQAIRRLKLGYDFQYELNAKGSTPAEVMGIDYKGTLKPELESYADKIRESSKKKFED 420

Query: 421 LISLRQQSSENTAKIESKRDHIASLQSHVNEHETQLISLKDEMRDYILRCSTESKKMVED 480
           +I L+QQS +   KIE K++ I +LQSH++E E Q+   K EM++Y  R + E+KKMVED
Sbjct: 421 MIILQQQSKDMATKIEDKKNRIDALQSHIDEVEAQINMFKKEMQEYGYRSTAEAKKMVED 480

Query: 481 VQQEAHNLDLVEGEAEESLKNAKLAHQEAIKQGEEEIHMCARELLSCLDSVSQYKAVVSC 540
           V  EAH LD+ E EA E LK ++   QEAI++ EEEI M A+EL   +DSVS+YK  V  
Sbjct: 481 VHIEAHKLDIAEKEAAEILKASQSRLQEAIQRSEEEIQMHAQELFMVVDSVSKYKEHVES 540

Query: 541 KISDMESCLSKAAAGISEAFKNSMPAE 566
           KIS++   LS  AA +S+A+K S+PA+
Sbjct: 541 KISEIRISLSDTAAAVSDAYKGSLPAQ 560

BLAST of Cucsa.049600 vs. TAIR10
Match: AT3G54630.1 (AT3G54630.1 Kinetochore protein Ndc80 (InterPro:IPR005550))

HSP 1 Score: 453.0 bits (1164), Expect = 2.7e-127
Identity = 240/572 (41.96%), Postives = 371/572 (64.86%), Query Frame = 1

Query: 1   MKGTA--RRRPKASFN-PPPPPTPQFR--------RDSDVSFASSRPSSIGMSRPSSASI 60
           M+G A  +RR    F   PPPP P           RDSD SFASSRPSSIG+    ++  
Sbjct: 1   MRGGAAGKRRTTVGFGGAPPPPPPSIEQQRHLFNSRDSDASFASSRPSSIGLGGRGASD- 60

Query: 61  DMYKERAVQISTVNTINSSLKELFSHSFNVTFKPFSPPSLKDLTETINLLLQCLDYP--P 120
               +R+  I  +N        L +H+F ++ +    PS+KD++ET+  LL  LDYP   
Sbjct: 61  ----DRSSMIRFINAF------LSTHNFPISIRGNPVPSVKDISETLKFLLSALDYPCDS 120

Query: 121 TKLEDDLPILLKSLGYPFKINKNILKNPPAPHQWYQILALLHWLVQVALYNYNLANDSNP 180
            K ++DL   LKS   PFKI K+ LK P  PH W  +LA++HWL ++A ++ +L ++S  
Sbjct: 121 IKWDEDLVFFLKSQKCPFKITKSSLKAPNTPHNWPTVLAVVHWLAELARFHQHLVSNSTS 180

Query: 181 IFTGHDVNIYVLNSYLHYMRGDDDSVEALDQEFMERLKKTRDDSRESLKELEEEVKVLEA 240
           +   + +N + + S+ H++RG+DD V  LD +F+ +L+  +    E++   E+    LEA
Sbjct: 181 VPEDNSMNFFAIQSFGHFIRGEDDKVNDLDSQFLGKLEAEKTSVAETISGCEKISGELEA 240

Query: 241 KVEALRSDPSQREVLEKQQSLLEEDVKKFHTMIAGYSEKMQEMKKLLEEKEKELDVKMEE 300
           K+E+LR  PS++E LEK ++ LE DV KF T++  Y+++   M+K++EEK KEL  K EE
Sbjct: 241 KLESLRKGPSKKESLEKVKADLENDVNKFRTIVVEYTDRNPAMEKVVEEKAKELKAKEEE 300

Query: 301 RNKICEENEELKKRVESQLFNPRDVERMRRELQAVERDIGDAEVSRNSWEEKSWDLDATL 360
           R +I  EN+ELKK VE Q F+  DV RMRRELQAVERD+ DAEV+R+ W++K+W+L++ +
Sbjct: 301 RERISVENKELKKSVELQNFSAADVNRMRRELQAVERDVADAEVARDGWDQKAWELNSQI 360

Query: 361 GNKFKELEAIAMECNQAMRRLKLDSCHQYVLNAKGSTPADVMGIDYKTTLKPALDSFAED 420
            N+F +++ +A++CNQA+RRLKLD   Q+ +N +G TPA VMG+DYK+ +KPAL S  + 
Sbjct: 361 RNQFHQIQTLAIDCNQALRRLKLDI--QFAVNERGETPAAVMGVDYKSVVKPALCSLCDG 420

Query: 421 IRRSSMMKLEELISLRQQSSENTAKIESKRDHIASLQSHVNEHETQLISLKDEMRDYILR 480
           I+ SS  K+EEL++L+   SE  +KIESKR  + S+Q  +N+ E ++  +K E ++   +
Sbjct: 421 IKGSSAEKVEELVTLQHHKSEMASKIESKRSLLGSIQLQINDLEEKMKLVKKETQELSTK 480

Query: 481 CSTESKKMVEDVQQEAHNLDLVEGEAEESLKNAKLAHQEAIKQGEEEIHMCARELLSCLD 540
           C  E+K +VE V+ EA NL++VE EA E +K ++L  QEA+K+ EEE+  CA +L + +D
Sbjct: 481 CDLEAKTLVESVKAEALNLEVVEKEAAEFVKASELRLQEAVKESEEEVQACAAQLFALID 540

Query: 541 SVSQYKAVVSCKISDMESCLSKAAAGISEAFK 560
           S+S+ K  +  KIS++++ ++  A+ +SE +K
Sbjct: 541 SISKQKEYMDSKISEIKTGVADTASAVSEIYK 559

BLAST of Cucsa.049600 vs. TAIR10
Match: AT1G63300.1 (AT1G63300.1 Myosin heavy chain-related protein)

HSP 1 Score: 55.8 bits (133), Expect = 9.7e-08
Identity = 61/299 (20.40%), Postives = 126/299 (42.14%), Query Frame = 1

Query: 183 LHYMRGDDDSVEALDQEFMERLKKTRD-----DSRESLKELEE--EVKVLEAKVEALRSD 242
           L   R  + SV    Q+  +RL +  D     + + ++K + E  E+++ + ++E +  D
Sbjct: 609 LRKTRWKNASVAGKLQDEFKRLSEQMDSMFTSNEKMAMKAMTEANELRMQKRQLEEMIKD 668

Query: 243 PSQREVLEKQQSLLEEDVKKFHTMIAGYSEKMQEMKKLLEEKEKELDVKMEERNKIC--- 302
            +    L   Q+  E  + +    ++  + +M+ M + L+EK  E+D +      +    
Sbjct: 669 ANDE--LRANQAEYEAKLHELSEKLSFKTSQMERMLENLDEKSNEIDNQKRHEEDVTANL 728

Query: 303 --------EENEELKKRVESQLFNPRDVERMRRELQAVERDIGDAEVSRNSWEEKSWDLD 362
                   EE E LKK  +S +      E +R +L+  ++ + +AE S      K  +L+
Sbjct: 729 NQEIKILKEEIENLKKNQDSLMLQAEQAENLRVDLEKTKKSVMEAEASLQRENMKKIELE 788

Query: 363 ATLGNKFKELEAIAMECNQAMRRLKLDSCHQYVLNAKGSTPADVMGIDYKTTLKPALDSF 422
           + +    KE E++A E              Q +  AK          D K T    L + 
Sbjct: 789 SKISLMRKESESLAAEL-------------QVIKLAK----------DEKETAISLLQTE 848

Query: 423 AEDIRRSSMMKLEELISLRQQSSENTAKIESKRDHIASLQSHVNEHETQLISLKDEMRD 464
            E +R        +   L+   SEN  ++E  +  +A ++S + + E  + +L+ ++++
Sbjct: 849 LETVR-------SQCDDLKHSLSENDLEMEKHKKQVAHVKSELKKKEETMANLEKKLKE 875

BLAST of Cucsa.049600 vs. TAIR10
Match: AT2G32240.1 (AT2G32240.1 FUNCTIONS IN: molecular_function unknown)

HSP 1 Score: 55.8 bits (133), Expect = 9.7e-08
Identity = 84/388 (21.65%), Postives = 162/388 (41.75%), Query Frame = 1

Query: 189 DDDSVEALDQEFMERLKKTRDDSRESLKELEEEVKVLEAKVEALRSDPSQREVLEK---- 248
           D +S +AL  EF E LK T+    ES KE+EE++  L+ +++ L    S+ E +E     
Sbjct: 229 DSESQKAL--EFSELLKSTK----ESAKEMEEKMASLQQEIKELNEKMSENEKVEAALKS 288

Query: 249 ---QQSLLEEDVKKFHTMIAGYSEKMQEMKKLLEEKEKELDVKMEERNKICEENEELK-- 308
              + + ++E++    + +    +K+   + L++E  +EL+ K    ++  EE   L+  
Sbjct: 289 SAGELAAVQEELALSKSRLLETEQKVSSTEALIDELTQELEQKKASESRFKEELSVLQDL 348

Query: 309 ----KRVESQLFNPRDV-ERMRRELQAVERDIGDAEVSRNSWEEKSWDLDATLGNKFKEL 368
               K ++++L     +  ++  EL+  E+++   E      EEK    +  L    KE 
Sbjct: 349 DAQTKGLQAKLSEQEGINSKLAEELK--EKEL--LESLSKDQEEKLRTANEKLAEVLKEK 408

Query: 369 EAIAMECNQAMRRLK--LDSCHQYVLNAKGSTPADVMGIDYKTTLKPALDSFAEDIRRSS 428
           EA+     +    +    + C++     K S        D   +   AL S A       
Sbjct: 409 EALEANVAEVTSNVATVTEVCNELEEKLKTS--------DENFSKTDALLSQALSNNSEL 468

Query: 429 MMKLEELISLRQQSSENTAKIESK----RDHIASLQSHVNEHETQLISLKDEMRDYILRC 488
             KL+ L  L  ++    A    K     D + S      E ++Q+  L+ +        
Sbjct: 469 EQKLKSLEELHSEAGSAAAAATQKNLELEDVVRSSSQAAEEAKSQIKELETKF------- 528

Query: 489 STESKKMVEDVQQEAHNLDLVEGEAEESLKNAKLAHQEAIKQGEEEIHMCARELLSCLDS 548
            T +++   +++Q+ + L L   +AE  LK       E   + +  I +   E       
Sbjct: 529 -TAAEQKNAELEQQLNLLQLKSSDAERELKEL----SEKSSELQTAIEVAEEEKKQATTQ 582

Query: 549 VSQYKAVVSCKISDMESCLSKAAAGISE 557
           + +YK     K S++E  L++++A  SE
Sbjct: 589 MQEYKQ----KASELELSLTQSSARNSE 582

BLAST of Cucsa.049600 vs. TAIR10
Match: AT5G27330.1 (AT5G27330.1 Prefoldin chaperone subunit family protein)

HSP 1 Score: 55.5 bits (132), Expect = 1.3e-07
Identity = 65/325 (20.00%), Postives = 151/325 (46.46%), Query Frame = 1

Query: 204 LKKTRDDSRESLKELEEEVKVLEAKVEALRSDPS----QREVLE-------KQQSLLEED 263
           L K + D    +++L+ E   L  K+E+ R + S    +R++++       ++ +LL+E 
Sbjct: 135 LFKEKSDRETEIRDLKREANGLIRKLESEREEFSRVCDERDLVKSGFDLQSEEMNLLKES 194

Query: 264 VKKFHTMIAGYSEKMQEMK----KLLEEKEKELDVKMEERNKICEENEELKKRVESQLFN 323
           V +         E++  +K    +L++E++K  +V +E  N+   E  EL + +E ++  
Sbjct: 195 VVRLEMREVSLGEEVGRLKCENGRLVKERKKREEV-IERGNR---ERSELVESLEEKV-- 254

Query: 324 PRDVERMRRELQAVERDIGDAEVSRNSWEEKSWDLDATLGNKFKELEAIAMECNQAMRRL 383
            R+++ ++RE++ V ++  + E+ R    E   +L+  LG+  + +E++  E  + +R  
Sbjct: 255 -REIDVLKREIEGVVKEKMEVEMVRRDQREMIVELEKKLGDMNEIVESLTKE-REGLR-- 314

Query: 384 KLDSCHQYVLNAKGSTPADVMGIDYKTTLKPALDSFAEDIRRSSMMKLEELISLRQQSSE 443
                              V+G      L+ +LD   E+ +     + E++  L ++ + 
Sbjct: 315 -----------------GQVVG------LEKSLDEVTEEAKA----RAEQINELVKEKTV 374

Query: 444 NTAKIESKRDHIASLQSHVNEHETQLISLKDEMRDYILRCSTESKKMVEDVQQEAHNLDL 503
             +++E       S++  +     Q  S K+++ + +LR   E  + V + + E   L  
Sbjct: 375 KESELEGLMVENNSIKKEIEMAMVQ-FSDKEKLVEQLLREKNELVQRVVNQEAEIVELSK 421

Query: 504 VEGEAEESLKNAKLAHQEAIKQGEE 514
           + GE + ++   +  + + IK GE+
Sbjct: 435 LAGEQKHAVAQLRKDYNDQIKNGEK 421

HSP 2 Score: 47.8 bits (112), Expect = 2.7e-05
Identity = 66/322 (20.50%), Postives = 139/322 (43.17%), Query Frame = 1

Query: 198 QEFMERLKKTRDDSRESLKELEEEVKVLEAKVEALRSDPSQREVLEKQQS----LLEEDV 257
           +E +ER  + R +  ESL+E   E+ VL+ ++E +  +  + E++ + Q      LE+ +
Sbjct: 227 EEVIERGNRERSELVESLEEKVREIDVLKREIEGVVKEKMEVEMVRRDQREMIVELEKKL 286

Query: 258 KKFHTMIAGYSEKMQEMKKLLEEKEKELDVKMEERNKICEENEELKKRVESQLFNPRDVE 317
              + ++   +++ + ++  +   EK LD   EE     E+  EL   V+ +     ++E
Sbjct: 287 GDMNEIVESLTKEREGLRGQVVGLEKSLDEVTEEAKARAEQINEL---VKEKTVKESELE 346

Query: 318 RMRRELQAVERDIGDAEVSRNSWE--------EKSWDLDATLGNKFK--ELEAIAMECNQ 377
            +  E  +++++I  A V  +  E        EK+  +   +  + +  EL  +A E   
Sbjct: 347 GLMVENNSIKKEIEMAMVQFSDKEKLVEQLLREKNELVQRVVNQEAEIVELSKLAGEQKH 406

Query: 378 AMRRLKLDSCHQY----VLNAKGSTPADVMGIDYKTTLKPALDSFAEDIRRSSMMKLEEL 437
           A+ +L+ D   Q      LN   S   D + +     ++      A D  + +M+ L+E 
Sbjct: 407 AVAQLRKDYNDQIKNGEKLNCNVSQLKDALAL---VEVERDNAGKALDEEKRNMVALKEK 466

Query: 438 ISLRQQSSENTAKIESKRDHIASLQSHVNEHETQLISLKDEMRDYILRCSTESKKMVEDV 497
           +   ++++E T K   K          +     +LI  K E+ +       E   + +D+
Sbjct: 467 VVALEKTNEATGKELEK----------IKAERGRLIKEKKELENRSESLRNEKAILQKDI 526

Query: 498 QQEAHNLDLVEGEAEESLKNAK 502
            +      +++ E E +  NAK
Sbjct: 527 VELKRATGVLKTELESAGTNAK 532

HSP 3 Score: 32.3 bits (72), Expect = 1.2e+00
Identity = 54/239 (22.59%), Postives = 100/239 (41.84%), Query Frame = 1

Query: 208 RDDSRESLKELEEEVKVLEAKVEALRSDPSQREVLEKQQSLLEEDVKKFHTMIAGYSEKM 267
           R D  + +K  E+    L   V  L+   +  EV         ++ K+   M+A   EK+
Sbjct: 409 RKDYNDQIKNGEK----LNCNVSQLKDALALVEVERDNAGKALDEEKR--NMVA-LKEKV 468

Query: 268 QEMKKLLEEKEKELDVKMEERNKICEENEELKKRVESQLFNPRDVERMRRELQAVERDIG 327
             ++K  E   KEL+    ER ++ +E +EL+ R ES          +R E   +++DI 
Sbjct: 469 VALEKTNEATGKELEKIKAERGRLIKEKKELENRSES----------LRNEKAILQKDI- 528

Query: 328 DAEVSRNSWEEKSWDLDATLGNKFKELEAIAMECNQAMRRLKLDSCHQYVLNAKGSTPAD 387
             E+ R +   K+            ELE+      Q++  LK  S     +  K      
Sbjct: 529 -VELKRATGVLKT------------ELESAGTNAKQSLTMLKSVSSLVCGIENKKDEKKR 588

Query: 388 VMGID-YKTTLKPALDSFAEDIRRSSMMKLEELISLRQQSSENTAKIESKRDHIASLQS 446
             G+D Y   L+    +F    + S + ++++ ++  + S E+  K +S    ++S+ S
Sbjct: 589 GKGMDSYSVQLEAIKKAFKN--KESMVEEMKKELAKMKHSVEDAHKKKSFWTLVSSVTS 614

BLAST of Cucsa.049600 vs. TAIR10
Match: AT1G13220.2 (AT1G13220.2 nuclear matrix constituent protein-related)

HSP 1 Score: 54.3 bits (129), Expect = 2.8e-07
Identity = 73/315 (23.17%), Postives = 145/315 (46.03%), Query Frame = 1

Query: 194 EALDQEFMERLKKTRDDSRESLKELEEEVKVLEAKVEALRSDPSQREVLEKQQSLLE-ED 253
           +A++++F +R+ +   D    LK ++E  K+++A+ + L         LEKQQ L + E 
Sbjct: 430 QAMNKKF-DRVNEKEMDLEAKLKTIKEREKIIQAEEKRLS--------LEKQQLLSDKES 489

Query: 254 VKKFHTMIAGYSEKMQEMKKLLEEKEKELDVKMEERNKICEENEELKKRVES----QLFN 313
           ++     I     +M + ++++EE+ K L++K EER +      ELK ++E     + F 
Sbjct: 490 LEDLQQEIEKIRAEMTKKEEMIEEECKSLEIKKEEREEYLRLQSELKSQIEKSRVHEEFL 549

Query: 314 PRDVERMRRELQAVERD---------IGDAEVSRNSWEEKSWDLDATL-GNKFKELE-AI 373
            ++VE +++E +  E++         + + E  R S E++ ++    L G + K+ E A+
Sbjct: 550 SKEVENLKQEKERFEKEWEILDEKQAVYNKERIRISEEKEKFERFQLLEGERLKKEESAL 609

Query: 374 AMECNQAMRRLKLDSCHQYVLNAKGSTPADVMGIDYKTTLKPALDSFAEDIRRSSMMKLE 433
            ++  Q +  ++L    +    A        +    K      +D   E +RR+  ++L+
Sbjct: 610 RVQIMQELDDIRL---QRESFEANMEHERSALQEKVKLEQSKVIDDL-EMMRRNLEIELQ 669

Query: 434 ELISLRQQS-SENTAKIESKRDHIASLQSHVNEHETQLISLKDEMRDYILRCSTES---- 486
           E     ++   +  A+ E KR  +A L S +N  +  L    +EM         ES    
Sbjct: 670 ERKEQDEKDLLDRMAQFEDKR--MAEL-SDINHQKQALNREMEEMMSKRSALQKESEEIA 728

BLAST of Cucsa.049600 vs. NCBI nr
Match: gi|778680142|ref|XP_004149971.2| (PREDICTED: kinetochore protein NDC80 homolog [Cucumis sativus])

HSP 1 Score: 1115.5 bits (2884), Expect = 0.0e+00
Identity = 565/565 (100.00%), Postives = 565/565 (100.00%), Query Frame = 1

Query: 1   MKGTARRRPKASFNPPPPPTPQFRRDSDVSFASSRPSSIGMSRPSSASIDMYKERAVQIS 60
           MKGTARRRPKASFNPPPPPTPQFRRDSDVSFASSRPSSIGMSRPSSASIDMYKERAVQIS
Sbjct: 1   MKGTARRRPKASFNPPPPPTPQFRRDSDVSFASSRPSSIGMSRPSSASIDMYKERAVQIS 60

Query: 61  TVNTINSSLKELFSHSFNVTFKPFSPPSLKDLTETINLLLQCLDYPPTKLEDDLPILLKS 120
           TVNTINSSLKELFSHSFNVTFKPFSPPSLKDLTETINLLLQCLDYPPTKLEDDLPILLKS
Sbjct: 61  TVNTINSSLKELFSHSFNVTFKPFSPPSLKDLTETINLLLQCLDYPPTKLEDDLPILLKS 120

Query: 121 LGYPFKINKNILKNPPAPHQWYQILALLHWLVQVALYNYNLANDSNPIFTGHDVNIYVLN 180
           LGYPFKINKNILKNPPAPHQWYQILALLHWLVQVALYNYNLANDSNPIFTGHDVNIYVLN
Sbjct: 121 LGYPFKINKNILKNPPAPHQWYQILALLHWLVQVALYNYNLANDSNPIFTGHDVNIYVLN 180

Query: 181 SYLHYMRGDDDSVEALDQEFMERLKKTRDDSRESLKELEEEVKVLEAKVEALRSDPSQRE 240
           SYLHYMRGDDDSVEALDQEFMERLKKTRDDSRESLKELEEEVKVLEAKVEALRSDPSQRE
Sbjct: 181 SYLHYMRGDDDSVEALDQEFMERLKKTRDDSRESLKELEEEVKVLEAKVEALRSDPSQRE 240

Query: 241 VLEKQQSLLEEDVKKFHTMIAGYSEKMQEMKKLLEEKEKELDVKMEERNKICEENEELKK 300
           VLEKQQSLLEEDVKKFHTMIAGYSEKMQEMKKLLEEKEKELDVKMEERNKICEENEELKK
Sbjct: 241 VLEKQQSLLEEDVKKFHTMIAGYSEKMQEMKKLLEEKEKELDVKMEERNKICEENEELKK 300

Query: 301 RVESQLFNPRDVERMRRELQAVERDIGDAEVSRNSWEEKSWDLDATLGNKFKELEAIAME 360
           RVESQLFNPRDVERMRRELQAVERDIGDAEVSRNSWEEKSWDLDATLGNKFKELEAIAME
Sbjct: 301 RVESQLFNPRDVERMRRELQAVERDIGDAEVSRNSWEEKSWDLDATLGNKFKELEAIAME 360

Query: 361 CNQAMRRLKLDSCHQYVLNAKGSTPADVMGIDYKTTLKPALDSFAEDIRRSSMMKLEELI 420
           CNQAMRRLKLDSCHQYVLNAKGSTPADVMGIDYKTTLKPALDSFAEDIRRSSMMKLEELI
Sbjct: 361 CNQAMRRLKLDSCHQYVLNAKGSTPADVMGIDYKTTLKPALDSFAEDIRRSSMMKLEELI 420

Query: 421 SLRQQSSENTAKIESKRDHIASLQSHVNEHETQLISLKDEMRDYILRCSTESKKMVEDVQ 480
           SLRQQSSENTAKIESKRDHIASLQSHVNEHETQLISLKDEMRDYILRCSTESKKMVEDVQ
Sbjct: 421 SLRQQSSENTAKIESKRDHIASLQSHVNEHETQLISLKDEMRDYILRCSTESKKMVEDVQ 480

Query: 481 QEAHNLDLVEGEAEESLKNAKLAHQEAIKQGEEEIHMCARELLSCLDSVSQYKAVVSCKI 540
           QEAHNLDLVEGEAEESLKNAKLAHQEAIKQGEEEIHMCARELLSCLDSVSQYKAVVSCKI
Sbjct: 481 QEAHNLDLVEGEAEESLKNAKLAHQEAIKQGEEEIHMCARELLSCLDSVSQYKAVVSCKI 540

Query: 541 SDMESCLSKAAAGISEAFKNSMPAE 566
           SDMESCLSKAAAGISEAFKNSMPAE
Sbjct: 541 SDMESCLSKAAAGISEAFKNSMPAE 565

BLAST of Cucsa.049600 vs. NCBI nr
Match: gi|659077518|ref|XP_008439248.1| (PREDICTED: kinetochore protein NDC80 homolog [Cucumis melo])

HSP 1 Score: 1080.5 bits (2793), Expect = 0.0e+00
Identity = 546/565 (96.64%), Postives = 555/565 (98.23%), Query Frame = 1

Query: 1   MKGTARRRPKASFNPPPPPTPQFRRDSDVSFASSRPSSIGMSRPSSASIDMYKERAVQIS 60
           MKGT RRRPKASFNPPPPPTPQFRRDSDVSFASSRPSSIGMSRP SASIDMYKERAVQIS
Sbjct: 1   MKGTGRRRPKASFNPPPPPTPQFRRDSDVSFASSRPSSIGMSRPPSASIDMYKERAVQIS 60

Query: 61  TVNTINSSLKELFSHSFNVTFKPFSPPSLKDLTETINLLLQCLDYPPTKLEDDLPILLKS 120
           TVNTINSSLKELFSHSFNVTFKPFSPPSLKDLTETINLLLQCLDYPPTKLEDDLPILLKS
Sbjct: 61  TVNTINSSLKELFSHSFNVTFKPFSPPSLKDLTETINLLLQCLDYPPTKLEDDLPILLKS 120

Query: 121 LGYPFKINKNILKNPPAPHQWYQILALLHWLVQVALYNYNLANDSNPIFTGHDVNIYVLN 180
           LGYPFKINKNILK PPAPHQWYQILALLHWLVQVALYNYNLANDSNP+F GHDVNIYVLN
Sbjct: 121 LGYPFKINKNILKTPPAPHQWYQILALLHWLVQVALYNYNLANDSNPVFMGHDVNIYVLN 180

Query: 181 SYLHYMRGDDDSVEALDQEFMERLKKTRDDSRESLKELEEEVKVLEAKVEALRSDPSQRE 240
           SYLHYMRGDDDSVEALDQEFMERLKKTRDDS ESLKELEEEVKVLEAKVEALRSDPSQRE
Sbjct: 181 SYLHYMRGDDDSVEALDQEFMERLKKTRDDSCESLKELEEEVKVLEAKVEALRSDPSQRE 240

Query: 241 VLEKQQSLLEEDVKKFHTMIAGYSEKMQEMKKLLEEKEKELDVKMEERNKICEENEELKK 300
           VLEKQQSLLEEDVKKFHTMIAGYSEKMQEMKKLLEEKEKEL+VKMEERNKICEENEELKK
Sbjct: 241 VLEKQQSLLEEDVKKFHTMIAGYSEKMQEMKKLLEEKEKELEVKMEERNKICEENEELKK 300

Query: 301 RVESQLFNPRDVERMRRELQAVERDIGDAEVSRNSWEEKSWDLDATLGNKFKELEAIAME 360
           RVESQLFNPRDVERMRRELQAVERDIGDAEVSRNSWEEKSWDLDATLGNKFKELEAIAME
Sbjct: 301 RVESQLFNPRDVERMRRELQAVERDIGDAEVSRNSWEEKSWDLDATLGNKFKELEAIAME 360

Query: 361 CNQAMRRLKLDSCHQYVLNAKGSTPADVMGIDYKTTLKPALDSFAEDIRRSSMMKLEELI 420
           CNQAMRRLKLDSCHQYVLNAKGSTPADVMGIDYK+T+KPALDSFAEDIRRSSMMKLEELI
Sbjct: 361 CNQAMRRLKLDSCHQYVLNAKGSTPADVMGIDYKSTVKPALDSFAEDIRRSSMMKLEELI 420

Query: 421 SLRQQSSENTAKIESKRDHIASLQSHVNEHETQLISLKDEMRDYILRCSTESKKMVEDVQ 480
           SLRQQSSENTAKI+SKR+ IASLQSH+NEHET+LISLKDEMRDYILRCSTESKKMVED Q
Sbjct: 421 SLRQQSSENTAKIDSKRNQIASLQSHINEHETKLISLKDEMRDYILRCSTESKKMVEDFQ 480

Query: 481 QEAHNLDLVEGEAEESLKNAKLAHQEAIKQGEEEIHMCARELLSCLDSVSQYKAVVSCKI 540
           QEAHNLDLVEGEAEESLKNAKLA+QEA KQ EEEIHMCARELLSCLDSVSQYKAVVSCKI
Sbjct: 481 QEAHNLDLVEGEAEESLKNAKLANQEATKQEEEEIHMCARELLSCLDSVSQYKAVVSCKI 540

Query: 541 SDMESCLSKAAAGISEAFKNSMPAE 566
           SDMESCLSKAAAGISEAFKNSMP E
Sbjct: 541 SDMESCLSKAAAGISEAFKNSMPVE 565

BLAST of Cucsa.049600 vs. NCBI nr
Match: gi|225455018|ref|XP_002279662.1| (PREDICTED: kinetochore protein ndc80 [Vitis vinifera])

HSP 1 Score: 642.5 bits (1656), Expect = 6.9e-181
Identity = 334/571 (58.49%), Postives = 432/571 (75.66%), Query Frame = 1

Query: 1   MKGTARRRPKASFNPPPPPTP------QFRRDSDVSFASSRPSSIGMSRPSSASIDMYKE 60
           MK T RRRPK SF PPPPP P      Q  RDSD SFA SRPSS+G+SR S+   D   +
Sbjct: 1   MKATGRRRPKDSFRPPPPPPPSVDHHRQLSRDSDASFAGSRPSSLGISRSSAG--DALTD 60

Query: 61  RAVQISTVNTINSSLKELFSHSFNVTFKPFSPPSLKDLTETINLLLQCLDYPPTKLEDDL 120
           R+ Q+S + TINS L    SHS  ++ KP   PS KD++ET+  L+  LD+P TKLEDDL
Sbjct: 61  RSYQLSAIRTINSFLA---SHSSPLSLKP-PLPSAKDISETLKFLISQLDFPTTKLEDDL 120

Query: 121 PILLKSLGYPFKINKNILKNPPAPHQWYQILALLHWLVQVALYNYNLANDSNPIFTGHDV 180
           PILLK L  P K+NK+ LK P  PH W  +L ++HWLVQ+ALYN +L ++        + 
Sbjct: 121 PILLKHLNCPIKLNKSALKAPGTPHAWPSLLGVMHWLVQIALYNDHLVSNLQCF---DNT 180

Query: 181 NIYVLNSYLHYMRGDDDSVEALDQEFMERLKKTRDDSRESLKELEEEVKVLEAKVEALRS 240
            +Y L+SYLHY+RGDDD +EA+D+EFM +L++ RD   E +K LE+EV   E ++E LR 
Sbjct: 181 FVYALDSYLHYIRGDDDGMEAVDREFMGKLERERDAVAEGVKALEKEVAEREGRLEELRL 240

Query: 241 DPSQREVLEKQQSLLEEDVKKFHTMIAGYSEKMQEMKKLLEEKEKELDVKMEERNKICEE 300
            PS +EV+EK++ +LEEDVKKFH +IA +S ++  ++K+LEEKEKEL VK+EE N+ICEE
Sbjct: 241 GPSAKEVVEKERGVLEEDVKKFHAIIAEFSGRIASVEKILEEKEKELGVKVEENNRICEE 300

Query: 301 NEELKKRVESQLFNPRDVERMRRELQAVERDIGDAEVSRNSWEEKSWDLDATLGNKFKEL 360
           NEELKKRVE Q FN RD ERM+RELQAVERDI +AEV+RN WEEKSWDLD T+G+KFKEL
Sbjct: 301 NEELKKRVELQTFNARDAERMKRELQAVERDITEAEVARNGWEEKSWDLDTTIGHKFKEL 360

Query: 361 EAIAMECNQAMRRLKLDSCHQYVLNAKGSTPADVMGIDYKTTLKPALDSFAEDIRRSSMM 420
           EA+++ECNQA+RRLKL +  QYVLNAKGS+PA+V+GIDYK+TLKPALDSFA+DI +SSM 
Sbjct: 361 EALSIECNQALRRLKLGNGLQYVLNAKGSSPAEVLGIDYKSTLKPALDSFADDINKSSMS 420

Query: 421 KLEELISLRQQSSENTAKIESKRDHIASLQSHVNEHETQLISLKDEMRDYILRCSTESKK 480
           KLEELISL+QQS EN AKIE+KR+ +A+LQS  +E E QLI LK E ++Y  RC+TE+KK
Sbjct: 421 KLEELISLQQQSVENAAKIEAKRNRLAALQSSSDEVEAQLIFLKKESQNYTSRCATEAKK 480

Query: 481 MVEDVQQEAHNLDLVEGEAEESLKNAKLAHQEAIKQGEEEIHMCARELLSCLDSVSQYKA 540
           +VEDV+ E HN+D+VE E  + L+ +KL  QEAIKQ EEEI MCA EL + +DSVS+YK 
Sbjct: 481 LVEDVEIETHNVDIVEREVADVLETSKLKLQEAIKQNEEEIQMCAWELYALVDSVSKYKE 540

Query: 541 VVSCKISDMESCLSKAAAGISEAFKNSMPAE 566
            ++ KIS+M+  LS+ A  +S+++K S+ A+
Sbjct: 541 HMASKISEMKCNLSETAGAVSDSYKGSLLAQ 562

BLAST of Cucsa.049600 vs. NCBI nr
Match: gi|225448101|ref|XP_002273942.1| (PREDICTED: kinetochore protein ndc80-like [Vitis vinifera])

HSP 1 Score: 637.9 bits (1644), Expect = 1.7e-179
Identity = 332/571 (58.14%), Postives = 430/571 (75.31%), Query Frame = 1

Query: 1   MKGTARRRPKASFNPPPPPTP------QFRRDSDVSFASSRPSSIGMSRPSSASIDMYKE 60
           MK T RRRPK S  PPPPP P      QF RDSD SFASSRPSS+G++R S+   D   +
Sbjct: 1   MKATGRRRPKDSIRPPPPPAPSVDHHRQFSRDSDASFASSRPSSLGINRSSAG--DALTD 60

Query: 61  RAVQISTVNTINSSLKELFSHSFNVTFKPFSPPSLKDLTETINLLLQCLDYPPTKLEDDL 120
           R+ Q+S + TINS L    SHS  ++ KP   PS KD++ET+  L+  LD+P TKLEDDL
Sbjct: 61  RSYQLSAIRTINSFLA---SHSSPLSLKP-PLPSAKDISETLKFLISQLDFPTTKLEDDL 120

Query: 121 PILLKSLGYPFKINKNILKNPPAPHQWYQILALLHWLVQVALYNYNLANDSNPIFTGHDV 180
           PILLK L  P K+NK+ LK P  PH W  +L ++HWLVQ+ALYN +L ++        + 
Sbjct: 121 PILLKHLNCPIKLNKSALKAPGTPHAWPPLLGVMHWLVQIALYNDHLVSNLQCF---DNT 180

Query: 181 NIYVLNSYLHYMRGDDDSVEALDQEFMERLKKTRDDSRESLKELEEEVKVLEAKVEALRS 240
            +Y L+SYLHY+RGDDD +EA+D+EFM +L+K RD   E +K LE+EV   E ++E LRS
Sbjct: 181 FVYALDSYLHYIRGDDDGMEAVDREFMGKLEKERDAVAEGVKALEKEVAEREGRLEELRS 240

Query: 241 DPSQREVLEKQQSLLEEDVKKFHTMIAGYSEKMQEMKKLLEEKEKELDVKMEERNKICEE 300
            PS +EV+EK + +LEEDVKKFH +IA +S ++  ++K+LEEKEKEL VK+EE N+ICEE
Sbjct: 241 GPSAKEVVEKVRGVLEEDVKKFHAIIAEFSGRIASVEKILEEKEKELGVKVEENNRICEE 300

Query: 301 NEELKKRVESQLFNPRDVERMRRELQAVERDIGDAEVSRNSWEEKSWDLDATLGNKFKEL 360
           NEELKKRVE Q FN RD ERM+RELQAVERDI +AEV+RN WEEKSWDLD T+G+KFKEL
Sbjct: 301 NEELKKRVELQTFNARDAERMKRELQAVERDITEAEVARNGWEEKSWDLDTTIGHKFKEL 360

Query: 361 EAIAMECNQAMRRLKLDSCHQYVLNAKGSTPADVMGIDYKTTLKPALDSFAEDIRRSSMM 420
           EA+++ECNQA+RRLKL +  QYVLNAKGS+P +V+GIDYK+ LKPALDSFA+DI +SSM 
Sbjct: 361 EALSIECNQALRRLKLGNGLQYVLNAKGSSPTEVLGIDYKSALKPALDSFADDINKSSMS 420

Query: 421 KLEELISLRQQSSENTAKIESKRDHIASLQSHVNEHETQLISLKDEMRDYILRCSTESKK 480
           KLEELISL+QQS EN AKIE+KR+ +A+LQS  +E E QL  LK E ++Y  RC+ E+KK
Sbjct: 421 KLEELISLQQQSVENAAKIEAKRNRLAALQSRSDEVEAQLNFLKKETQNYTSRCAMEAKK 480

Query: 481 MVEDVQQEAHNLDLVEGEAEESLKNAKLAHQEAIKQGEEEIHMCARELLSCLDSVSQYKA 540
           +VEDV+ E HN+D+VE E  + L+ +KL  QEAIKQ EEEI +CA EL + +DSVS+YK 
Sbjct: 481 LVEDVEIETHNVDIVEREVADVLETSKLKLQEAIKQNEEEIQICAWELYALVDSVSKYKE 540

Query: 541 VVSCKISDMESCLSKAAAGISEAFKNSMPAE 566
            ++ KIS+M+S LS+ A  +S+++K S+ A+
Sbjct: 541 HMASKISEMKSNLSETAGAVSDSYKGSLSAQ 562

BLAST of Cucsa.049600 vs. NCBI nr
Match: gi|147771819|emb|CAN66776.1| (hypothetical protein VITISV_035303 [Vitis vinifera])

HSP 1 Score: 636.3 bits (1640), Expect = 5.0e-179
Identity = 332/571 (58.14%), Postives = 429/571 (75.13%), Query Frame = 1

Query: 1   MKGTARRRPKASFNPPPPPTP------QFRRDSDVSFASSRPSSIGMSRPSSASIDMYKE 60
           MK T RRRPK S  PPPPP P      QF RDSD SFASSRPSS+G++R S+   D   +
Sbjct: 1   MKATGRRRPKDSIRPPPPPAPSVDHHRQFSRDSDASFASSRPSSLGINRSSAG--DALTD 60

Query: 61  RAVQISTVNTINSSLKELFSHSFNVTFKPFSPPSLKDLTETINLLLQCLDYPPTKLEDDL 120
           R+ Q+S + TINS L    SHS  ++ KP   PS KD++ET+  L+  LD+P TKLEDDL
Sbjct: 61  RSYQLSAIRTINSFLA---SHSSPLSLKP-PLPSAKDISETLKFLISQLDFPTTKLEDDL 120

Query: 121 PILLKSLGYPFKINKNILKNPPAPHQWYQILALLHWLVQVALYNYNLANDSNPIFTGHDV 180
           PILLK L  P K+NK+ LK P  PH W  +L ++HWLVQ+ALYN +L ++        + 
Sbjct: 121 PILLKHLNCPIKLNKSALKAPGTPHAWPPLLGVMHWLVQIALYNDHLVSNLQCF---DNT 180

Query: 181 NIYVLNSYLHYMRGDDDSVEALDQEFMERLKKTRDDSRESLKELEEEVKVLEAKVEALRS 240
            +Y L+SYLHY+RGDDD +EA+D+EFM +L+  RD   E +K LE+EV   E ++E LRS
Sbjct: 181 FVYALDSYLHYIRGDDDGMEAVDREFMGKLEXERDAVAEGVKALEKEVAEREGRLEELRS 240

Query: 241 DPSQREVLEKQQSLLEEDVKKFHTMIAGYSEKMQEMKKLLEEKEKELDVKMEERNKICEE 300
            PS +EV+EK++ +LEEDVKKFH +IA +S ++  ++K+LEEKEKEL VK+EE N+ICEE
Sbjct: 241 GPSAKEVVEKERGVLEEDVKKFHAIIAEFSGRIASVEKILEEKEKELGVKVEENNRICEE 300

Query: 301 NEELKKRVESQLFNPRDVERMRRELQAVERDIGDAEVSRNSWEEKSWDLDATLGNKFKEL 360
           NEELKKRVE Q FN RD ERM+RELQAVERDI +AEV+RN WEEKSWDLD T+G+KFKEL
Sbjct: 301 NEELKKRVELQTFNARDAERMKRELQAVERDITEAEVARNGWEEKSWDLDTTIGHKFKEL 360

Query: 361 EAIAMECNQAMRRLKLDSCHQYVLNAKGSTPADVMGIDYKTTLKPALDSFAEDIRRSSMM 420
           EA+++ECNQA+RRLKL +  QYVLNAKGS+P +V+GIDYK+ LKPALDSFA+DI +SSM 
Sbjct: 361 EALSIECNQALRRLKLGNGLQYVLNAKGSSPXEVLGIDYKSXLKPALDSFADDINKSSMS 420

Query: 421 KLEELISLRQQSSENTAKIESKRDHIASLQSHVNEHETQLISLKDEMRDYILRCSTESKK 480
           KLEELISL+QQS EN AKIE+KR+ +A+LQS  +E E QLI LK E ++Y  RC+TE+KK
Sbjct: 421 KLEELISLQQQSVENAAKIEAKRNRLAALQSXSDEVEAQLIFLKKESQNYTSRCATEAKK 480

Query: 481 MVEDVQQEAHNLDLVEGEAEESLKNAKLAHQEAIKQGEEEIHMCARELLSCLDSVSQYKA 540
           +VEDV+ E HN+D VE E  + L+ +KL  QEAIKQ EEEI  CA EL + +DSVS+YK 
Sbjct: 481 LVEDVEIETHNVDXVEREVADVLETSKLKLQEAIKQNEEEIQXCAWELYALVDSVSKYKE 540

Query: 541 VVSCKISDMESCLSKAAAGISEAFKNSMPAE 566
            ++ KIS+M+  LS+ A  +S+++K S+ A+
Sbjct: 541 HMASKISEMKCNLSETAGAVSDSYKGSLLAQ 562