Cucsa.023010 (gene) Cucumber (Gy14) v1

BLAST of Cucsa.023010 vs. Swiss-Prot
Match: GBA2_HUMAN (Non-lysosomal glucosylceramidase OS=Homo sapiens GN=GBA2 PE=1 SV=2)

HSP 1 Score: 343.6 bits (880), Expect = 7.0e-93
Identity = 182/401 (45.39%), Postives = 240/401 (59.85%), Query Frame = 1

Query: 505 LDELRDSVASNSAFGVNLLQKGEENVGQFLYLEGVEYTMWNTYDVHFYSSFAIIMLFPKL 564
           L+ L DS+       +  L+    + G+F YLEG EY M+NTYDVHFY+SFA+IML+PKL
Sbjct: 495 LEVLEDSLPEELGRNMCHLRPTLRDYGRFGYLEGQEYRMYNTYDVHFYASFALIMLWPKL 554

Query: 565 ELSIQRDFAAAVMMHDPSKMQLLDNGKWEARNVLGAVPHDIGVND--PWFEVNGYNLYNT 624
           ELS+Q D A A +  D ++ + L +G          +PHDIG  D  PW  VN Y +++T
Sbjct: 555 ELSLQYDMALATLREDLTRRRYLMSGVMAPVKRRNVIPHDIGDPDDEPWLRVNAYLIHDT 614

Query: 625 DRWKDLNPKFVLQIYRDVVATGDMKFAKAVWPPVYLAIAYMDQFDRDGDGMIENDGFPDQ 684
             WKDLN KFVLQ+YRD   TGD  F K +WP     +    +FD+D DG+IEN G+ DQ
Sbjct: 615 ADWKDLNLKFVLQVYRDYYLTGDQNFLKDMWPVCLAVMESEMKFDKDHDGLIENGGYADQ 674

Query: 685 TYDTWSVSGVSAYSGGLWVAALQAASALARVADEKDAEHYFWFKFQKAKRAYEK-LWNGS 744
           TYD W  +G SAY GGLW+AA+     +A +   +D +  F     + + AYE+ LWNG 
Sbjct: 675 TYDGWVTTGPSAYCGGLWLAAVAVMVQMAALCGAQDIQDKFSSILSRGQEAYERLLWNGR 734

Query: 745 YFNYDSSGGSSSSSIQADQLAGQWYARASGL----LPIVDEDKAKSALAKVYNYNVMKVK 804
           Y+NYDSS    S S+ +DQ AGQW+ +A GL      +        AL  ++  NV    
Sbjct: 735 YYNYDSSSRPQSRSVMSDQCAGQWFLKACGLGEGDTEVFPTQHVVRALQTIFELNVQAFA 794

Query: 805 DGKRGAVNGMLPDGTIDFSSMQSREIWSGVTYAVAASMIHEDMTDMAFRTAEGIHEAAWS 864
            G  GAVNGM P G  D SS+QS E+W GV Y +AA+MI E +T   F+TAEG +   W 
Sbjct: 795 GGAMGAVNGMQPHGVPDKSSVQSDEVWVGVVYGLAATMIQEGLTWEGFQTAEGCYRTVW- 854

Query: 865 EDGLGYNFQTPEAWTTTDRYRSLCYMRPLAIWAMQWAFSEK 899
            + LG  FQTPEA+     +RSL YMRPL+IWAMQ A  ++
Sbjct: 855 -ERLGLAFQTPEAYCQQRVFRSLAYMRPLSIWAMQLALQQQ 893

HSP 2 Score: 254.6 bits (649), Expect = 4.3e-66
Identity = 148/435 (34.02%), Postives = 223/435 (51.26%), Query Frame = 1

Query: 32  QTPSLTWQRKLNREGTS----LWQFRLHLKEIIHLAPVGYRLFRY-IREESVKGRGAMIN 91
           Q P   W+  L  E T          + L  +I    +G R  ++  R+  V+ +   I+
Sbjct: 80  QVPPFGWRICLAHEFTEKRKPFQANNVSLSNMIKHIGMGLRYLQWWYRKTHVEKKTPFID 139

Query: 92  PFIRRRITDTHAIPLGGLGSGSIGRSYRGEFQRWQLFPRKCEDKPILANQFSVFVSRPNA 151
                 +   +  PLGG+G G+I R +RG+F RWQL P   + + ++A+QF+V + R   
Sbjct: 140 MINSVPLRQIYGCPLGGIGGGTITRGWRGQFCRWQLNPGMYQHRTVIADQFTVCLRREGQ 199

Query: 152 EKYSTVLCAQSPETQREVESSGIGSWDWNLKGHSSTYHALYPRAWTIYDGEPDPELRIVC 211
             Y  VL  + P   R        SW+W L G+ + YHALYPRAWT+Y   P   + + C
Sbjct: 200 TVYQQVLSLERPSVLR--------SWNWGLCGYFAFYHALYPRAWTVYQ-LPGQNVTLTC 259

Query: 212 RQISPVIPHNYKESSYPVSVFTFTLHNSGKTDADVSLLFTWANSVGGLSEYSGNHINSR- 271
           RQI+P++PH+Y++SS PV VF + + N G    DVS++F+  N +GG  +  G   N   
Sbjct: 260 RQITPILPHDYQDSSLPVGVFVWDVENEGDEALDVSIMFSMRNGLGGGDDAPGGLWNEPF 319

Query: 272 --TKKKDGVHTVLLHHKTASGFPPVTYAIAAQEGNGVHVSDCPCFVISGNSQGISAKDMW 331
              +  + V  +LLHH T     P T A+AA+      V+    F      Q +     W
Sbjct: 320 CLERSGETVRGLLLHHPTLPN--PYTMAVAARVTAATTVTHITAFDPDSTGQQV-----W 379

Query: 332 LEIKEHGSFDRLKFADMSMPSEVGSSIGAAVSASVTVSSDSVRTVTFSLSWDCPEVNF-C 391
            ++ + G  D       S P++ G  I  AV  S  +       + FSL+WD P + F  
Sbjct: 380 QDLLQDGQLDSP--TGQSTPTQKGVGIAGAVCVSSKLRPRGQCRLEFSLAWDMPRIMFGA 439

Query: 392 RGKTYHRRYTKFYGNLGDAAADIARDAILEHHHWESQIDAWQRPVLEDKRFPKWYPVTLF 451
           +G+ ++RRYT+F+G  GDAA  ++  A+  +  WE +I AWQ PVL+D+  P WY   LF
Sbjct: 440 KGQVHYRRYTRFFGQDGDAAPALSHYALCRYAEWEERISAWQSPVLDDRSLPAWYKSALF 496

Query: 452 NELYYLNAGGTIWTD 458
           NELY+L  GGT+W +
Sbjct: 500 NELYFLADGGTVWLE 496

BLAST of Cucsa.023010 vs. Swiss-Prot
Match: GBA2_MOUSE (Non-lysosomal glucosylceramidase OS=Mus musculus GN=Gba2 PE=1 SV=2)

HSP 1 Score: 342.0 bits (876), Expect = 2.0e-92
Identity = 182/396 (45.96%), Postives = 238/396 (60.10%), Query Frame = 1

Query: 510 DSVASNSAFGVNLLQKGEENVGQFLYLEGVEYTMWNTYDVHFYSSFAIIMLFPKLELSIQ 569
           DS+       +  L+   ++ G+F YLEG EY M+NTYDVHFY+SFA++ML+PKLELS+Q
Sbjct: 491 DSLPEGLGGSMRQLRSTLQDYGRFGYLEGQEYRMYNTYDVHFYASFALVMLWPKLELSLQ 550

Query: 570 RDFAAAVMMHDPSKMQLLDNGKWEARNVLGAVPHDIGVND--PWFEVNGYNLYNTDRWKD 629
            D A A +  D ++ + L +G          +PHDIG  D  PW  VN Y +++T  WKD
Sbjct: 551 YDMALATLKEDLTRRRYLMSGVVAPVKRRNVIPHDIGDPDDEPWLRVNAYLIHDTADWKD 610

Query: 630 LNPKFVLQIYRDVVATGDMKFAKAVWPPVYLAIAYMDQFDRDGDGMIENDGFPDQTYDTW 689
           LN KFVLQIYRD   TGD  F + +WP     +    +FD+D DG+IEN G+ DQTYD W
Sbjct: 611 LNLKFVLQIYRDYYLTGDQGFLEDMWPVCLAVMESEMKFDKDQDGLIENGGYADQTYDAW 670

Query: 690 SVSGVSAYSGGLWVAALQAASALARVADEKDAEHYFWFKFQKAKRAYEK-LWNGSYFNYD 749
             +G SAY GGLW+AA+     +A +   +D +  F     + + AYE+ LWNG Y+NYD
Sbjct: 671 VTTGPSAYCGGLWLAAVAVMVQMAVLCGAQDVQERFASILCRGREAYERLLWNGRYYNYD 730

Query: 750 SSGGSSSSSIQADQLAGQWYARASGL----LPIVDEDKAKSALAKVYNYNVMKVKDGKRG 809
           SS    S SI +DQ AGQW+ RA GL      +        AL  ++  NV     G  G
Sbjct: 731 SSSHPQSRSIMSDQCAGQWFLRACGLGEGDTEVFPTLHVVRALQTIFELNVQAFAGGAMG 790

Query: 810 AVNGMLPDGTIDFSSMQSREIWSGVTYAVAASMIHEDMTDMAFRTAEGIHEAAWSEDGLG 869
           AVNGM P G  D SS+QS E+W GV Y +AA+MI E +T   FRTAEG +   W  + LG
Sbjct: 791 AVNGMHPHGVPDRSSVQSDEVWVGVVYGLAATMIQEGLTWEGFRTAEGCYRTVW--ERLG 850

Query: 870 YNFQTPEAWTTTDRYRSLCYMRPLAIWAMQWAFSEK 899
             FQTPEA+     +RSL YMRPL+IWAMQ A  ++
Sbjct: 851 LAFQTPEAYCQQQVFRSLAYMRPLSIWAMQLALQQQ 884

HSP 2 Score: 258.1 bits (658), Expect = 3.9e-67
Identity = 146/417 (35.01%), Postives = 225/417 (53.96%), Query Frame = 1

Query: 54  LHLKEIIHLAPVGYRLFRY-IREESVKGRGAMINPFIRRRITDTHAIPLGGLGSGSIGRS 113
           + L  ++    +G R  ++  R+  V+ +   I+      +   +  PLGG+G G+I R 
Sbjct: 97  ISLSNLVKHLGMGLRYLKWWYRKTHVEKKTPFIDMLNSLPLRQIYGCPLGGIGGGTITRG 156

Query: 114 YRGEFQRWQLFPRKCEDKPILANQFSVFVSRPNAEKYSTVLCAQSPETQREVESSGIGSW 173
           +RG+F RWQL P   + + ++A+QF V + R     Y  VL  + P   R        SW
Sbjct: 157 WRGQFCRWQLNPGMYQHQTVIADQFIVCLRRDGRTVYQQVLSLELPNVLR--------SW 216

Query: 174 DWNLKGHSSTYHALYPRAWTIYDGEPDPELRIVCRQISPVIPHNYKESSYPVSVFTFTLH 233
           +W L G+ + YHALYPRAWT+Y   P   + + CRQ++P++PH+Y++SS PV VF + + 
Sbjct: 217 NWGLCGYFAFYHALYPRAWTVYQ-LPGQNVTLTCRQVTPILPHDYQDSSLPVGVFVWDVE 276

Query: 234 NSGKTDADVSLLFTWANSVGGLSEYSGNHINSRTKKKDGVHTV---LLHHKTASGFPPVT 293
           N G    DVS+ F+  N +GG  + +G+  N   + + G  TV   LLHH T     P T
Sbjct: 277 NEGDETLDVSITFSMRNGLGGEDDAAGSLWNEPFRLEQGGTTVQGLLLHHPTPPN--PYT 336

Query: 294 YAIAAQEGNGVHVSDCPCFVISGNSQGISAKDMWLEIKEHGSFDRLKFADMSMPSEVGSS 353
            A+AA+      V+    F  +G  Q +     W ++ + G  D    A  S P++ G  
Sbjct: 337 MAVAARCTADTTVTHTTAFDPNGTGQQV-----WQDLLQDGQLDSP--AGQSTPTQKGEG 396

Query: 354 IGAAVSASVTVSSDSVRTVTFSLSWDCPEVNF-CRGKTYHRRYTKFYGNLGDAAADIARD 413
           I  AV  S  +   S   + FSL+WD P++ F  + + ++RRYT+F+G+ GD A  ++  
Sbjct: 397 IAGAVCVSSKLLPRSRCCLEFSLAWDMPKIMFGAKSQVHYRRYTRFFGSDGDVAPALSHY 456

Query: 414 AILEHHHWESQIDAWQRPVLEDKRFPKWYPVTLFNELYYLNAGGTIWTDGSLPIQSL 466
           A+  +  WE +I AWQ PVL+D+  P WY   LFNELY+L  GGT+W +  +P  SL
Sbjct: 457 ALCHYADWEDRISAWQNPVLDDRTLPAWYKSALFNELYFLADGGTVWLE--VPADSL 493

BLAST of Cucsa.023010 vs. Swiss-Prot
Match: GBA2_RAT (Non-lysosomal glucosylceramidase OS=Rattus norvegicus GN=Gba2 PE=2 SV=2)

HSP 1 Score: 339.0 bits (868), Expect = 1.7e-91
Identity = 178/378 (47.09%), Postives = 231/378 (61.11%), Query Frame = 1

Query: 528 ENVGQFLYLEGVEYTMWNTYDVHFYSSFAIIMLFPKLELSIQRDFAAAVMMHDPSKMQLL 587
           ++ G+F YLEG EY M+NTYDVHFY+SFA++ML+PKLELS+Q D A A    D ++ + L
Sbjct: 509 QDYGRFGYLEGQEYRMYNTYDVHFYASFALVMLWPKLELSLQYDMALATFKEDLTRRRYL 568

Query: 588 DNGKWEARNVLGAVPHDIGVND--PWFEVNGYNLYNTDRWKDLNPKFVLQIYRDVVATGD 647
            +G          +PHDIG  D  PW  VN Y +++T  WKDLN KFVLQ+YRD   TGD
Sbjct: 569 MSGVVAPVKRRNVIPHDIGDPDDEPWLRVNAYLIHDTADWKDLNLKFVLQVYRDYYLTGD 628

Query: 648 MKFAKAVWPPVYLAIAYMDQFDRDGDGMIENDGFPDQTYDTWSVSGVSAYSGGLWVAALQ 707
             F K +WP     +    +FD+D DG+IEN G+ DQTYD W  +G SAY GGLW+AA+ 
Sbjct: 629 QGFLKDMWPVCLAVMESEMKFDKDQDGLIENGGYADQTYDGWVTTGPSAYCGGLWLAAVA 688

Query: 708 AASALARVADEKDAEHYFWFKFQKAKRAYEK-LWNGSYFNYDSSGGSSSSSIQADQLAGQ 767
               +A +   +D +  F     + + AYE+ LWNG Y+NYDSS    S S+ +DQ AGQ
Sbjct: 689 VMVQMAVLCGAQDVQDKFSSILCRGREAYERLLWNGRYYNYDSSSQPQSRSVMSDQCAGQ 748

Query: 768 WYARASGL----LPIVDEDKAKSALAKVYNYNVMKVKDGKRGAVNGMLPDGTIDFSSMQS 827
           W+ RA GL      +        AL  ++  NV     G  GAVNGM P G  D SS+QS
Sbjct: 749 WFLRACGLGEGDTEVFPTLHVVRALKTIFELNVQAFAGGAMGAVNGMQPHGVPDRSSVQS 808

Query: 828 REIWSGVTYAVAASMIHEDMTDMAFRTAEGIHEAAWSEDGLGYNFQTPEAWTTTDRYRSL 887
            E+W GV Y +AA+MI E +T   FRTAEG +   W  + LG  FQTPEA+     +RSL
Sbjct: 809 DEVWVGVVYGLAATMIQEGLTWEGFRTAEGCYRTVW--ERLGLAFQTPEAYCQQRVFRSL 868

Query: 888 CYMRPLAIWAMQWAFSEK 899
            YMRPL+IWAMQ A  ++
Sbjct: 869 AYMRPLSIWAMQLALQQQ 884

HSP 2 Score: 260.8 bits (665), Expect = 6.0e-68
Identity = 143/407 (35.14%), Postives = 224/407 (55.04%), Query Frame = 1

Query: 56  LKEIIHLAPVGYRLFRY-IREESVKGRGAMINPFIRRRITDTHAIPLGGLGSGSIGRSYR 115
           L  ++    +G R  ++  R+  V+ +   I+ F    +   +  PLGG+G G+I R +R
Sbjct: 99  LSNLVKHFGMGLRYLKWWYRKTQVEKKTPFIDMFNSVPLRQIYGCPLGGIGGGTITRGWR 158

Query: 116 GEFQRWQLFPRKCEDKPILANQFSVFVSRPNAEKYSTVLCAQSPETQREVESSGIGSWDW 175
           G+F RWQL P   + + ++A+QF V + R     Y  VL  + P   R        SW+W
Sbjct: 159 GQFCRWQLNPGMYQHQTVIADQFIVCLRRDGRTVYQQVLSLELPSVLR--------SWNW 218

Query: 176 NLKGHSSTYHALYPRAWTIYDGEPDPELRIVCRQISPVIPHNYKESSYPVSVFTFTLHNS 235
            L G+ + YHALYPRAWT+Y   P   + + CRQI+P++PH+Y++SS PV VF + + N 
Sbjct: 219 GLCGYFAFYHALYPRAWTVYQ-LPGQNVTLTCRQITPILPHDYQDSSLPVGVFVWDVENE 278

Query: 236 GKTDADVSLLFTWANSVGGLSEYSGNHINSRTK-KKDG--VHTVLLHHKTASGFPPVTYA 295
           G    DVS++F+  N +GG  + +G   N   + ++DG  V  +LLHH T     P T A
Sbjct: 279 GDETLDVSIMFSMRNGLGGEDDAAGGLWNEPFRLEQDGTTVQGLLLHHPTPPN--PYTMA 338

Query: 296 IAAQEGNGVHVSDCPCFVISGNSQGISAKDMWLEIKEHGSFDRLKFADMSMPSEVGSSIG 355
           +AA+     H +D      +      + + +W ++ + G  D    A  S P++ G  + 
Sbjct: 339 VAAR-----HTADTTVTYTTAFDPDSTGQQVWQDLLQDGQLDSP--AGQSTPTQRGEGVA 398

Query: 356 AAVSASVTVSSDSVRTVTFSLSWDCPEVNF-CRGKTYHRRYTKFYGNLGDAAADIARDAI 415
            AV AS  +       + FSL+WD P + F  +G+ ++RRYT+F+G+ GD A  ++  A+
Sbjct: 399 GAVCASSKLLPRGRCCLEFSLAWDMPRIMFGAKGQVHYRRYTRFFGSDGDVAPALSHYAL 458

Query: 416 LEHHHWESQIDAWQRPVLEDKRFPKWYPVTLFNELYYLNAGGTIWTD 458
            ++  WE+ I AWQ PVL+D+  P WY   LFNELY+L  GGT+W +
Sbjct: 459 CQYAGWENSISAWQNPVLDDRSLPAWYKSALFNELYFLADGGTVWLE 487

BLAST of Cucsa.023010 vs. Swiss-Prot
Match: C3390_DROME (Non-lysosomal glucosylceramidase OS=Drosophila melanogaster GN=CG33090 PE=1 SV=1)

HSP 1 Score: 223.0 bits (567), Expect = 1.4e-56
Identity = 129/425 (30.35%), Postives = 213/425 (50.12%), Query Frame = 1

Query: 53  RLHLKEIIHLAPVGYRLFRYIREESVKGRGAMINPFIRRRITDTHAIPLGGLGSGSIGRS 112
           R  +++ + + P+  R   Y  + S +GR   ++ +        + +P+GG+G G+IGR 
Sbjct: 72  RASIRQTLPMVPLVCRYAAYYWKVSREGRRVYMDYYYMENGKQIYGVPIGGIGGGTIGRG 131

Query: 113 YRGEFQRWQLFPRKCEDKPILANQFSVFVSRPNA--------EKYSTVLCAQSPETQREV 172
           Y GEF R+Q+ P   E   +LANQF V +  P           K ST      P+   + 
Sbjct: 132 YAGEFCRFQMRPGIYEYNVVLANQFIVTIKDPKGCTIFQSLLSKCSTRDKTSDPDGDPDG 191

Query: 173 ESSG-------------IGSWDWNLKGHSSTYHALYPRAWTIYDGEPDPELRIVCRQISP 232
           E +              + +W  N++    +Y  LYPR+WT YD      +R+ CRQ+SP
Sbjct: 192 ERTKCQLPNCSSRAKQPLSAWHSNIEDTRCSYTGLYPRSWTEYDLS-HYGVRLTCRQVSP 251

Query: 233 VIPHNYKESSYPVSVFTFTLHNSGKTDADVSLLFTWANSVGGLSEYSGNHINSRTKKKDG 292
           VIPH Y+ESS P +VF +++ N    +  VS+ FT+ N  G   + +     S+   +  
Sbjct: 252 VIPHEYRESSLPCAVFVWSVENVCDQERKVSITFTFKNGTGNKKQDAEGGAESQLISEGN 311

Query: 293 VHTVLLHHKTASGFPPVTYAIAAQEGNGVHVSDCPCFVISGNSQGISAKDMWLEIKEHGS 352
              V +  K +    P +Y +A +    + ++ CP F  +GN +      +W ++KEHG 
Sbjct: 312 AKGVSIRQKISE--MPCSYNLACRVLPEISITRCPQFDPAGNGE-----QLWAQLKEHGQ 371

Query: 353 FDRLKFADMSMPSEVGSSIGAAVSASVTVSSDSVRTVTFSLSWDCPEVNFCRG-KTYHRR 412
                 ++     ++G ++   V+     S D    + F L+WD P++ F R  +T+ R 
Sbjct: 372 LSEHPTSEALKTKDIGVAVCGQVALKPMASHD----LEFVLAWDMPKIQFPRKMQTHTRY 431

Query: 413 YTKFYGNLGDAAADIARDAILEHHHWESQIDAWQRPVLEDKRFPKWYPVTLFNELYYLNA 456
           YTK++ + GD+   I   A+ ++  WE  IDAWQRP+L D+  P WY   +FN+LY+++ 
Sbjct: 432 YTKYFDDSGDSGPRICEYALRQYSTWERLIDAWQRPILNDETLPDWYKCAIFNQLYFISD 484

HSP 2 Score: 203.0 bits (515), Expect = 1.5e-50
Identity = 109/260 (41.92%), Postives = 160/260 (61.54%), Query Frame = 1

Query: 648 FAKAVWPPVYLAIAYMDQFDRDGDGMIENDGFPDQTYDTWSVSGVSAYSGGLWVAALQAA 707
           + KA++      +    ++D+D DG+IEN   PDQTYD+W + G SAY  GLW+AALQA 
Sbjct: 687 YLKAMYASCKAIMERTIEYDKDNDGLIENTKMPDQTYDSWVMDGPSAYCSGLWLAALQAM 746

Query: 708 SALARVADEKDAEHYFWFKFQKAKRAY-EKLWNGSYFNYDSSGGSSSSSIQADQLAGQWY 767
           SA+A + D+ +    +    +K KR+  EKLWNGSY+ +D S  S   +I ADQL G WY
Sbjct: 747 SAMATILDQPNDCLRYQDILEKGKRSLEEKLWNGSYYRFDLS-HSHRDTIMADQLCGHWY 806

Query: 768 ARASGL-LPIVDEDKAKSALAKVYNYNVMKVKDGKRGAVNGMLPD-------GTIDFSSM 827
            ++ G    I  ++  ++AL ++Y+ NVM   +G  GA NG + +       G +D S++
Sbjct: 807 LKSCGFDYEIYPKENVRTALKRIYDNNVMGFHEGNIGAANGFIANASEPTKPGHVDNSNI 866

Query: 828 QSREIWSGVTYAVAASMIHEDMTDMAFRTAEGIHEAAWSEDGLGYNFQTPEAWTTTDRYR 887
           Q+ E+W GV YA+AA+MI E M + AF+TA G+++       +G NF+TPEA     RYR
Sbjct: 867 QAEEVWPGVVYALAATMIQEGMFEEAFQTAGGMYKTL--SQRIGMNFETPEALYGEKRYR 926

Query: 888 SLCYMRPLAIWAMQWAFSEK 899
           S+ YMRPL+IW+MQ A   +
Sbjct: 927 SIGYMRPLSIWSMQVALERR 943

HSP 3 Score: 112.8 bits (281), Expect = 2.0e-23
Identity = 58/137 (42.34%), Postives = 87/137 (63.50%), Query Frame = 1

Query: 531 GQFLYLEGVEYTMWNTYDVHFYSSFAIIMLFPKLELSIQRDFAAAVMMHDPSKMQLLDNG 590
           G+F YLEG EY M+NTYDVHFY+S A+  L+P L++S+Q DF  A+        ++L +G
Sbjct: 505 GRFGYLEGHEYRMYNTYDVHFYASPALAHLWPNLQVSLQYDFKDAIAAELNDTRKMLYDG 564

Query: 591 KWEARNVLGAVPHDIGVND--PWFEVNGYNLYNTDRWKDLNPKFVLQIYRDVVATGDMKF 650
           K   R V   VPHD+G  D  P+  +N YN+++ + WKDLN KFVLQ+YRD     ++  
Sbjct: 565 KVMPRKVKNCVPHDLGDPDEEPFTLINCYNIHDVNDWKDLNTKFVLQVYRDYYVLNELAQ 624

Query: 651 AKAVWPPVYLAIAYMDQ 666
           A++     + +I ++D+
Sbjct: 625 AQSDNASKFSSIEFIDK 641

BLAST of Cucsa.023010 vs. TrEMBL
Match: A0A0A0KPF7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G011660 PE=4 SV=1)

HSP 1 Score: 1580.5 bits (4091), Expect = 0.0e+00
Identity = 766/776 (98.71%), Postives = 766/776 (98.71%), Query Frame = 1

Query: 1   MSEGKKLGNGLVEQDEDINKSLTEK----------TGIDPGQTPSLTWQRKLNREGTSLW 60
           MSEGKKLGNGLVEQDEDINKSLTEK          TGIDPGQTPSLTWQRKLNREGTSLW
Sbjct: 55  MSEGKKLGNGLVEQDEDINKSLTEKVKWCIQSFFMTGIDPGQTPSLTWQRKLNREGTSLW 114

Query: 61  QFRLHLKEIIHLAPVGYRLFRYIREESVKGRGAMINPFIRRRITDTHAIPLGGLGSGSIG 120
           QFRLHLKEIIHLAPVGYRLFRYIREESVKGRGAMINPFIRRRITDTHAIPLGGLGSGSIG
Sbjct: 115 QFRLHLKEIIHLAPVGYRLFRYIREESVKGRGAMINPFIRRRITDTHAIPLGGLGSGSIG 174

Query: 121 RSYRGEFQRWQLFPRKCEDKPILANQFSVFVSRPNAEKYSTVLCAQSPETQREVESSGIG 180
           RSYRGEFQRWQLFPRKCEDKPILANQFSVFVSRPNAEKYSTVLCAQSPETQREVESSGIG
Sbjct: 175 RSYRGEFQRWQLFPRKCEDKPILANQFSVFVSRPNAEKYSTVLCAQSPETQREVESSGIG 234

Query: 181 SWDWNLKGHSSTYHALYPRAWTIYDGEPDPELRIVCRQISPVIPHNYKESSYPVSVFTFT 240
           SWDWNLKGHSSTYHALYPRAWTIYDGEPDPELRIVCRQISPVIPHNYKESSYPVSVFTFT
Sbjct: 235 SWDWNLKGHSSTYHALYPRAWTIYDGEPDPELRIVCRQISPVIPHNYKESSYPVSVFTFT 294

Query: 241 LHNSGKTDADVSLLFTWANSVGGLSEYSGNHINSRTKKKDGVHTVLLHHKTASGFPPVTY 300
           LHNSGKTDADVSLLFTWANSVGGLSEYSGNHINSRTKKKDGVHTVLLHHKTASGFPPVTY
Sbjct: 295 LHNSGKTDADVSLLFTWANSVGGLSEYSGNHINSRTKKKDGVHTVLLHHKTASGFPPVTY 354

Query: 301 AIAAQEGNGVHVSDCPCFVISGNSQGISAKDMWLEIKEHGSFDRLKFADMSMPSEVGSSI 360
           AIAAQEGNGVHVSDCPCFVISGNSQGISAKDMWLEIKEHGSFDRLKFADMSMPSEVGSSI
Sbjct: 355 AIAAQEGNGVHVSDCPCFVISGNSQGISAKDMWLEIKEHGSFDRLKFADMSMPSEVGSSI 414

Query: 361 GAAVSASVTVSSDSVRTVTFSLSWDCPEVNFCRGKTYHRRYTKFYGNLGDAAADIARDAI 420
           GAAVSASVTVSSDSVRTVTFSLSWDCPEVNFCRGKTYHRRYTKFYGNLGDAAADIARDAI
Sbjct: 415 GAAVSASVTVSSDSVRTVTFSLSWDCPEVNFCRGKTYHRRYTKFYGNLGDAAADIARDAI 474

Query: 421 LEHHHWESQIDAWQRPVLEDKRFPKWYPVTLFNELYYLNAGGTIWTDGSLPIQSLVSIGE 480
           LEHHHWESQIDAWQRPVLEDKRFPKWYPVTLFNELYYLNAGGTIWTDGSLPIQSLVSIGE
Sbjct: 475 LEHHHWESQIDAWQRPVLEDKRFPKWYPVTLFNELYYLNAGGTIWTDGSLPIQSLVSIGE 534

Query: 481 RGFCLDQCRSGDSVIYESHENDTANDILGRMTSRLDELRDSVASNSAFGVNLLQKGEENV 540
           RGFCLDQCRSGDSVIYESHENDTANDILGRMTSRLDELRDSVASNSAFGVNLLQKGEENV
Sbjct: 535 RGFCLDQCRSGDSVIYESHENDTANDILGRMTSRLDELRDSVASNSAFGVNLLQKGEENV 594

Query: 541 GQFLYLEGVEYTMWNTYDVHFYSSFAIIMLFPKLELSIQRDFAAAVMMHDPSKMQLLDNG 600
           GQFLYLEGVEYTMWNTYDVHFYSSFAIIMLFPKLELSIQRDFAAAVMMHDPSKMQLLDNG
Sbjct: 595 GQFLYLEGVEYTMWNTYDVHFYSSFAIIMLFPKLELSIQRDFAAAVMMHDPSKMQLLDNG 654

Query: 601 KWEARNVLGAVPHDIGVNDPWFEVNGYNLYNTDRWKDLNPKFVLQIYRDVVATGDMKFAK 660
           KWEARNVLGAVPHDIGVNDPWFEVNGYNLYNTDRWKDLNPKFVLQIYRDVVATGDMKFAK
Sbjct: 655 KWEARNVLGAVPHDIGVNDPWFEVNGYNLYNTDRWKDLNPKFVLQIYRDVVATGDMKFAK 714

Query: 661 AVWPPVYLAIAYMDQFDRDGDGMIENDGFPDQTYDTWSVSGVSAYSGGLWVAALQAASAL 720
           AVWPPVYLAIAYMDQFDRDGDGMIENDGFPDQTYDTWSVSGVSAYSGGLWVAALQAASAL
Sbjct: 715 AVWPPVYLAIAYMDQFDRDGDGMIENDGFPDQTYDTWSVSGVSAYSGGLWVAALQAASAL 774

Query: 721 ARVADEKDAEHYFWFKFQKAKRAYEKLWNGSYFNYDSSGGSSSSSIQADQLAGQWY 767
           ARVADEKDAEHYFWFKFQKAKRAYEKLWNGSYFNYDSSGGSSSSSIQADQLAGQWY
Sbjct: 775 ARVADEKDAEHYFWFKFQKAKRAYEKLWNGSYFNYDSSGGSSSSSIQADQLAGQWY 830

BLAST of Cucsa.023010 vs. TrEMBL
Match: B9SS77_RICCO (Non-lysosomal glucosylceramidase OS=Ricinus communis GN=RCOM_0618840 PE=3 SV=1)

HSP 1 Score: 1461.8 bits (3783), Expect = 0.0e+00
Identity = 709/951 (74.55%), Postives = 802/951 (84.33%), Query Frame = 1

Query: 1   MSEGKKLGNGLVEQDEDINKSLTEKTGIDPGQTPSLTWQRKLNREGTSLWQFRLHLKEII 60
           MSEG+   NG  ++D + + SL EK  +DPG   SLTWQRKLN E  +L QF L  +E  
Sbjct: 1   MSEGEIPANGC-QEDREPSDSLLEK--VDPGSPASLTWQRKLNSEDIALSQFNLSFQEKF 60

Query: 61  HLAPVGYRLFRYIREESVKGRGAMINPFIRRRITDTHAIPLGGLGSGSIGRSYRGEFQRW 120
            LAPVG RL+R IREE+ KGR ++INPF++R IT  H IPLGG+GSGSIGRSY+GEFQRW
Sbjct: 61  QLAPVGIRLWRLIREETAKGRVSIINPFLKRFITSCHGIPLGGIGSGSIGRSYKGEFQRW 120

Query: 121 QLFPRKCEDKPILANQFSVFVSRPNAEKYSTVLCAQSPETQREVESSGIGSWDWNLKGHS 180
           QLFPR CE+KP+LANQFSVFVSR + EKYS+VLC ++PE   E   SGIGSWDWNLKG +
Sbjct: 121 QLFPRICEEKPVLANQFSVFVSRSSGEKYSSVLCPRNPEVLMEPAISGIGSWDWNLKGDN 180

Query: 181 STYHALYPRAWTIYDGEPDPELRIVCRQISPVIPHNYKESSYPVSVFTFTLHNSGKTDAD 240
           STYHALYPRAWTIYDGEPDPELRIVCRQISP+IPHNYKESSYPVSVFTFTL+NSGKT AD
Sbjct: 181 STYHALYPRAWTIYDGEPDPELRIVCRQISPIIPHNYKESSYPVSVFTFTLYNSGKTTAD 240

Query: 241 VSLLFTWANSVGGLSEYSGNHINSRTKKKDGVHTVLLHHKTASGFPPVTYAIAAQEGNGV 300
           VSLLFTW NSVGG SEYSG H NS T  +DGVH VLLHHKTA GFPPVT+AIAAQE N V
Sbjct: 241 VSLLFTWTNSVGGNSEYSGQHFNSTTMMEDGVHAVLLHHKTAEGFPPVTFAIAAQETNDV 300

Query: 301 HVSDCPCFVISGNSQGISAKDMWLEIKEHGSFDRLKFADMSMPSEVGSSIGAAVSASVTV 360
           HVS CP FVISGN QGI+AKDMW E+KEHGSFD LK A  S PSE GSSIGAA++ASVT+
Sbjct: 301 HVSKCPRFVISGNCQGITAKDMWHEVKEHGSFDNLKSAGTSGPSEPGSSIGAAIAASVTI 360

Query: 361 SSDSVRTVTFSLSWDCPEVNFCRGKTYHRRYTKFYGNLGDAAADIARDAILEHHHWESQI 420
             D++R+VTFSLSWDCPEV F  G+TYHRRYTKFY   GDAAA IA DAILEH  WESQI
Sbjct: 361 PPDAIRSVTFSLSWDCPEVYFMGGRTYHRRYTKFYSTHGDAAARIAHDAILEHGLWESQI 420

Query: 421 DAWQRPVLEDKRFPKWYPVTLFNELYYLNAGGTIWTDGSLPIQSLVSIGERGFCLDQCRS 480
            AWQRP+LEDKR P+WYP+TLFNELYYLN+GGTIWTDGS P  +LVSI    F LD   +
Sbjct: 421 VAWQRPILEDKRLPEWYPITLFNELYYLNSGGTIWTDGSPPYHNLVSIRGSKFSLDTSGA 480

Query: 481 G-DSVIYESHENDTANDILGRMTSRLDELRDSVASNSAFGVNLLQKGEENVGQFLYLEGV 540
           G  S+I  +HENDTA +ILGRMTS L+++   VASNSAFG NLLQ+GEEN+GQFLYLEG+
Sbjct: 481 GLKSIIDVTHENDTAVNILGRMTSALEQIHAHVASNSAFGTNLLQEGEENIGQFLYLEGI 540

Query: 541 EYTMWNTYDVHFYSSFAIIMLFPKLELSIQRDFAAAVMMHDPSKMQLLDNGKWEARNVLG 600
           EY MWNTYDVHFYSSFA++MLFPKLELS+QRDFAAAVMMHDPSKMQLL +G+W  R VLG
Sbjct: 541 EYHMWNTYDVHFYSSFALVMLFPKLELSVQRDFAAAVMMHDPSKMQLLHDGQWVCRKVLG 600

Query: 601 AVPHDIGVNDPWFEVNGYNLYNTDRWKDLNPKFVLQIYRDVVATGDMKFAKAVWPPVYLA 660
           AVPHDIG+NDPW+EVN Y+LYNTDRWKDLNPKFVLQ+YRDVVATGD KFA+AVWP VY+A
Sbjct: 601 AVPHDIGINDPWYEVNAYSLYNTDRWKDLNPKFVLQVYRDVVATGDKKFAEAVWPSVYIA 660

Query: 661 IAYMDQFDRDGDGMIENDGFPDQTYDTWSVSGVSAYSGGLWVAALQAASALARVADEKDA 720
           +AYMDQFDRDGDGMIENDGFPDQTYDTWSVSGVSAYSGGLWVAALQAASALAR   +K +
Sbjct: 661 MAYMDQFDRDGDGMIENDGFPDQTYDTWSVSGVSAYSGGLWVAALQAASALAREVGDKGS 720

Query: 721 EHYFWFKFQKAKRAYEKLWNGSYFNYDSSGGSSSSSIQADQLAGQWYARASGLLPIVDED 780
           E YFW +FQKAK  Y+KLWNGSYFNYD+SGG +SSSIQADQLAGQWYARA GL PIVD+D
Sbjct: 721 EDYFWARFQKAKDVYDKLWNGSYFNYDNSGGRNSSSIQADQLAGQWYARACGLFPIVDKD 780

Query: 781 KAKSALAKVYNYNVMKVKDGKRGAVNGMLPDGTIDFSSMQSREIWSGVTYAVAASMIHED 840
           KA+SAL KVYNYNV+KVKDGKRGA+NGMLPDG +D SSMQSREIWSGVTYA+AA+MI ED
Sbjct: 781 KARSALEKVYNYNVLKVKDGKRGAINGMLPDGKVDLSSMQSREIWSGVTYALAATMIQED 840

Query: 841 MTDMAFRTAEGIHEAAWSEDGLGYNFQTPEAWTTTDRYRSLCYMRPLAIWAMQWAFSEKV 900
           M DMAF TA GI+EAAWSE GLGY+FQTPEAW   D+YRSLCYMRPLAIWAMQWA S   
Sbjct: 841 MLDMAFHTASGIYEAAWSERGLGYSFQTPEAWNNVDQYRSLCYMRPLAIWAMQWALSRPK 900

Query: 901 SVIEELK-ELDSDAISRHHARFSKVARYLKLPEDGTSSSVLQTVYDYTLKR 950
              EE++ E++ D +  HHA F+KVAR+L+LPE   S  +LQ++++YT K+
Sbjct: 901 LEKEEMEMEVNEDYLLPHHAGFTKVARFLRLPEGEESLGLLQSLFNYTCKK 948

BLAST of Cucsa.023010 vs. TrEMBL
Match: M5XQ56_PRUPE (Non-lysosomal glucosylceramidase OS=Prunus persica GN=PRUPE_ppa000954mg PE=3 SV=1)

HSP 1 Score: 1454.1 bits (3763), Expect = 0.0e+00
Identity = 693/953 (72.72%), Postives = 807/953 (84.68%), Query Frame = 1

Query: 1   MSEGKKLGNGLVEQDEDINKSLTEKTGIDPGQTPSLTWQRKLNREGTSLWQFRLHLKEII 60
           M E K L NG VE+D++ + S  +K  +DPG+  SLTW+RKLN +G     F L LKEII
Sbjct: 1   MLEKKILENGFVERDKEDSDSSFDK--VDPGKPTSLTWKRKLNSKGNDPLPFTLSLKEII 60

Query: 61  HLAPVGYRLFRYIREESVKGRGAMINPFIRRRITDTHAIPLGGLGSGSIGRSYRGEFQRW 120
            +AP+G RL+R++REE+  GR A INPF++R +T +H +PLGG+G+GSIGRSY GEFQRW
Sbjct: 61  QMAPIGVRLWRHLREEATNGREAFINPFVKRLLTSSHGVPLGGIGAGSIGRSYSGEFQRW 120

Query: 121 QLFPRKCEDKPILANQFSVFVSRPNAEKYSTVLCAQSPETQREVESSGIGSWDWNLKGHS 180
           QLFP K E+KP+LA+QFSVFVSR N EKY TVLC + PE  +E E SGIGSWDWNL G +
Sbjct: 121 QLFPGKFEEKPVLADQFSVFVSRTNGEKYCTVLCPRRPEVLKESEVSGIGSWDWNLNGDN 180

Query: 181 STYHALYPRAWTIYDGEPDPELRIVCRQISPVIPHNYKESSYPVSVFTFTLHNSGKTDAD 240
           STYHAL+PRAW++Y+GEPDP L+IVCRQISP IPHNYKESS+PVSVFTFTL+NSGKT AD
Sbjct: 181 STYHALFPRAWSVYEGEPDPALKIVCRQISPFIPHNYKESSFPVSVFTFTLYNSGKTAAD 240

Query: 241 VSLLFTWANSVGGLSEYSGNHINSRTKKKDGVHTVLLHHKTASGFPPVTYAIAAQEGNGV 300
           V+LLFTWANSVGGLSE+SG+H NSR   KDGVH VLLHHKTA+G PPVT+AIAA+E +G+
Sbjct: 241 VTLLFTWANSVGGLSEFSGHHFNSRAVIKDGVHGVLLHHKTANGLPPVTFAIAAEETDGI 300

Query: 301 HVSDCPCFVISGNSQGISAKDMWLEIKEHGSFDRLKFADMSMPSEVGSSIGAAVSASVTV 360
           HVS+CPCFVISG+S+GI+AKDMW EIKEHGSFDRL   + S  SE GSSIGAA++ASVTV
Sbjct: 301 HVSECPCFVISGDSKGITAKDMWTEIKEHGSFDRLNSTETSSDSEPGSSIGAAIAASVTV 360

Query: 361 SSDSVRTVTFSLSWDCPEVNFCRGKTYHRRYTKFYGNLGDAAADIARDAILEHHHWESQI 420
             D VRTVTFSL+WDCPEV F  GKTYHRRYTKFYG  GDA A+IA DAILEHHHWESQI
Sbjct: 361 PPDGVRTVTFSLAWDCPEVKFMGGKTYHRRYTKFYGTHGDAVANIAHDAILEHHHWESQI 420

Query: 421 DAWQRPVLEDKRFPKWYPVTLFNELYYLNAGGTIWTDGSLPIQSLVSIGERGFCLDQCRS 480
           ++WQRPVL+DKR P+WYP+TLFNELYYLN+GGT+WTDGS P+ SL SIG R F LD+   
Sbjct: 421 ESWQRPVLDDKRLPEWYPITLFNELYYLNSGGTVWTDGSPPVHSLTSIGGRKFSLDRSSL 480

Query: 481 G-DSVIYESHENDTANDILGRMTSRLDELRDSVASNSAFGVNLLQKGEENVGQFLYLEGV 540
           G  S+I    +NDTA DILGRMTS L+++   +ASNSAFG NLLQ+GEEN+GQFLYLEG+
Sbjct: 481 GLKSIIDVPPQNDTAIDILGRMTSILEQVHTPIASNSAFGTNLLQEGEENIGQFLYLEGI 540

Query: 541 EYTMWNTYDVHFYSSFAIIMLFPKLELSIQRDFAAAVMMHDPSKMQLLDNGKWEARNVLG 600
           EY MWNTYDVHFYSSFA++MLFPKL+LSIQRDFAAAVMMHDPSKM+LL +GKW  R VLG
Sbjct: 541 EYQMWNTYDVHFYSSFALVMLFPKLQLSIQRDFAAAVMMHDPSKMRLLHDGKWVQRKVLG 600

Query: 601 AVPHDIGVNDPWFEVNGYNLYNTDRWKDLNPKFVLQIYRDVVATGDMKFAKAVWPPVYLA 660
           AVPHDIG++DPWFEVN YNLYNTDRWKDLNPKFVLQ+YRDVVATGD KFA+AVWP VY+A
Sbjct: 601 AVPHDIGLHDPWFEVNAYNLYNTDRWKDLNPKFVLQVYRDVVATGDKKFAQAVWPSVYVA 660

Query: 661 IAYMDQFDRDGDGMIENDGFPDQTYDTWSVSGVSAYSGGLWVAALQAASALARVADEKDA 720
           +AYM+QFD+DGDGMIENDGFPDQTYDTWSVSGVSAYSGGLW+AALQAASA+AR   +K +
Sbjct: 661 MAYMEQFDKDGDGMIENDGFPDQTYDTWSVSGVSAYSGGLWLAALQAASAMAREVGDKGS 720

Query: 721 EHYFWFKFQKAKRAYEKLWNGSYFNYDSSGGSSSSSIQADQLAGQWYARASGLLPIVDED 780
           E YFW KFQKAK  YEKLWNGSYFNYD+SG SSSSSIQADQLAGQWYARA GLLPIVDED
Sbjct: 721 EDYFWGKFQKAKVVYEKLWNGSYFNYDNSGQSSSSSIQADQLAGQWYARACGLLPIVDED 780

Query: 781 KAKSALAKVYNYNVMKVKDGKRGAVNGMLPDGTIDFSSMQSREIWSGVTYAVAASMIHED 840
           KA+SAL KVY YNV+K KDG++GAVNGMLPDG +D SS+QSREIWSGVTYAVAA+MIHED
Sbjct: 781 KARSALEKVYTYNVLKFKDGRQGAVNGMLPDGKVDMSSLQSREIWSGVTYAVAATMIHED 840

Query: 841 MTDMAFRTAEGIHEAAWSEDGLGYNFQTPEAWTTTDRYRSLCYMRPLAIWAMQWAFSEKV 900
           M DMAF TA G++EAAWS++GLGY FQTPEAWTT+  +RSL YMRPLAIW+M WA S+  
Sbjct: 841 MIDMAFHTAGGVYEAAWSKEGLGYAFQTPEAWTTSGEFRSLAYMRPLAIWSMHWALSKPA 900

Query: 901 SVIEELK-ELDSDAISRHHARFSKVARYLKLPEDGTSSSVLQTVYDYTLKRFF 952
              +E+K E D  ++ RH   F+KVA+ LKLP++  S S+LQ V+DYT KR +
Sbjct: 901 LFKQEMKLEADEGSLHRHKVGFAKVAQLLKLPQEEESRSILQAVFDYTCKRLW 951

BLAST of Cucsa.023010 vs. TrEMBL
Match: D7TGQ9_VITVI (Non-lysosomal glucosylceramidase OS=Vitis vinifera GN=VIT_12s0035g01220 PE=3 SV=1)

HSP 1 Score: 1451.8 bits (3757), Expect = 0.0e+00
Identity = 688/945 (72.80%), Postives = 807/945 (85.40%), Query Frame = 1

Query: 9   NGLVEQDEDINKSLTEKTGIDPGQTPSLTWQRKLNREGTSLWQFRLHLKEIIHLAPVGYR 68
           NGL E + + + S  E+  +DPG+  SLTWQRKLN +G +  +F+++L+E  HLAP+G+R
Sbjct: 4   NGLEEGEREPSNSSIEEIKVDPGKPGSLTWQRKLNSDGNAPVEFKINLRETFHLAPIGFR 63

Query: 69  LFRYIREESVKGRGAMINPFIRRRITDTHAIPLGGLGSGSIGRSYRGEFQRWQLFPRKCE 128
           L+R++REE+ KGRGAMI+PF++R I+ +  +PLGG+G+GSIGRSY+GEFQR+QLFP   E
Sbjct: 64  LWRHVREETAKGRGAMIDPFVKRYISSSQGVPLGGIGAGSIGRSYKGEFQRFQLFPITSE 123

Query: 129 DKPILANQFSVFVSRPNAEKYSTVLCAQSPETQREVESSGIGSWDWNLKGHSSTYHALYP 188
           ++P+L NQFSVFVSRPN EKYSTVLC QSPE  +E   SGIGSWDWNL G+ STY ALYP
Sbjct: 124 NEPVLENQFSVFVSRPNGEKYSTVLCRQSPEALKECPPSGIGSWDWNLNGNKSTYLALYP 183

Query: 189 RAWTIYDGEPDPELRIVCRQISPVIPHNYKESSYPVSVFTFTLHNSGKTDADVSLLFTWA 248
           RAWT+YDGEPDP L+IVCRQISP+IPHNYKESS+PV+VFTFTL NSGKT AD++LLFTWA
Sbjct: 184 RAWTVYDGEPDPALKIVCRQISPIIPHNYKESSFPVAVFTFTLFNSGKTAADITLLFTWA 243

Query: 249 NSVGGLSEYSGNHINSRTKKKDGVHTVLLHHKTASGFPPVTYAIAAQEGNGVHVSDCPCF 308
           NSVGG+S  SG H+NS+   KDGV  VLLHHKTA+G PPVTYAIAAQE +GVH+S+CPCF
Sbjct: 244 NSVGGVSGLSGQHLNSKFMMKDGVRGVLLHHKTANGRPPVTYAIAAQEMDGVHISECPCF 303

Query: 309 VISGNSQGISAKDMWLEIKEHGSFDRLKFADMSMPSEVGSSIGAAVSASVTVSSDSVRTV 368
            ISG++ GI+AKDMW EIKEHGSFDRL  A+ SMPSE+GSS+GAAV+AS+T+ SDS +TV
Sbjct: 304 FISGDTPGITAKDMWNEIKEHGSFDRLNSAETSMPSELGSSVGAAVAASLTIPSDSEQTV 363

Query: 369 TFSLSWDCPEVNFCRGKTYHRRYTKFYGNLGDAAADIARDAILEHHHWESQIDAWQRPVL 428
           TFSL+WDCPE+NF + +TY+RRYTKFYG  GDAAA IA DAIL+H HWESQI+AWQ+PVL
Sbjct: 364 TFSLAWDCPEINFSKERTYYRRYTKFYGTRGDAAAKIAHDAILDHGHWESQIEAWQKPVL 423

Query: 429 EDKRFPKWYPVTLFNELYYLNAGGTIWTDGSLPIQSLVSIGERGFCLDQCRSG-DSVIYE 488
           EDKRFP+WYP+TLFNELYYLN+GGT+WTDGS P+ S  SI ER F LD+ RS   + +  
Sbjct: 424 EDKRFPEWYPITLFNELYYLNSGGTVWTDGSPPVHSFTSIIERKFSLDRSRSDLKNTVSI 483

Query: 489 SHENDTANDILGRMTSRLDELRDSVASNSAFGVNLLQKGEENVGQFLYLEGVEYTMWNTY 548
           SH NDTA DIL RMTS L+++   V SNSAFG NLLQ+GEEN+GQFLYLEGVEY MWNT 
Sbjct: 484 SHHNDTAVDILERMTSVLEQVHTPVTSNSAFGPNLLQQGEENIGQFLYLEGVEYFMWNTN 543

Query: 549 DVHFYSSFAIIMLFPKLELSIQRDFAAAVMMHDPSKMQLLDNGKWEARNVLGAVPHDIGV 608
           DVHFYSSFA+IMLFPKLELSIQRDFAA+VMMHDPSKM+LL NGKW +R VLGAVPHD+G 
Sbjct: 544 DVHFYSSFALIMLFPKLELSIQRDFAASVMMHDPSKMKLLCNGKWVSRKVLGAVPHDVGN 603

Query: 609 NDPWFEVNGYNLYNTDRWKDLNPKFVLQIYRDVVATGDMKFAKAVWPPVYLAIAYMDQFD 668
            DPWFEVNGYNLYNTDRWKDLNPKFVLQ+YRDVVATGD  FAKAVWP VY+A+AYM+QFD
Sbjct: 604 YDPWFEVNGYNLYNTDRWKDLNPKFVLQVYRDVVATGDKNFAKAVWPSVYVALAYMNQFD 663

Query: 669 RDGDGMIENDGFPDQTYDTWSVSGVSAYSGGLWVAALQAASALARVADEKDAEHYFWFKF 728
           +DGDGMIEN+GFPDQTYDTWSVSGVSAYSGGLWVAALQAASALARV  +K +E YFWFKF
Sbjct: 664 KDGDGMIENEGFPDQTYDTWSVSGVSAYSGGLWVAALQAASALARVVGDKGSEDYFWFKF 723

Query: 729 QKAKRAYEKLWNGSYFNYDSSGGSSSSSIQADQLAGQWYARASGLLPIVDEDKAKSALAK 788
           QKAK  Y+KLWNGSYFNYD S GSSSSSIQADQLAGQWYARA GL PIVDEDKAKSAL K
Sbjct: 724 QKAKGVYQKLWNGSYFNYDDSDGSSSSSIQADQLAGQWYARACGLSPIVDEDKAKSALEK 783

Query: 789 VYNYNVMKVKDGKRGAVNGMLPDGTIDFSSMQSREIWSGVTYAVAASMIHEDMTDMAFRT 848
           VY+YNV+KV  GKRGAVNGMLPDG +D ++MQSREIWSGVTY VAA+MIHE + DMAF+T
Sbjct: 784 VYHYNVLKVGGGKRGAVNGMLPDGKVDTTTMQSREIWSGVTYGVAATMIHEGLVDMAFQT 843

Query: 849 AEGIHEAAWSEDGLGYNFQTPEAWTTTDRYRSLCYMRPLAIWAMQWAFSE-KVSVIEELK 908
           A G++EAAWS++GLGY+FQTPE+W T D+YRSLCYMRPLAIWAMQWAFS+ K+   E   
Sbjct: 844 ASGVYEAAWSQEGLGYSFQTPESWNTDDQYRSLCYMRPLAIWAMQWAFSQPKLHKYEANP 903

Query: 909 ELDSDAISRHHARFSKVARYLKLPEDGTSSSVLQTVYDYTLKRFF 952
           E++ D++   HA FS+VAR LKLP++  S S LQ +YDYT KR +
Sbjct: 904 EMNEDSLVLQHAGFSRVARLLKLPDEDVSRSALQVIYDYTCKRMW 948

BLAST of Cucsa.023010 vs. TrEMBL
Match: A0A061DW00_THECC (Non-lysosomal glucosylceramidase OS=Theobroma cacao GN=TCM_006124 PE=3 SV=1)

HSP 1 Score: 1448.0 bits (3747), Expect = 0.0e+00
Identity = 695/951 (73.08%), Postives = 798/951 (83.91%), Query Frame = 1

Query: 1   MSEGKKLGNGLVEQDEDINKSLTEKTGIDPGQTPSLTWQRKLNREGTSLWQFRLHLKEII 60
           MSE K L NG  E D+D +     K  +DP +   LTW RKLN EG     F L  +E +
Sbjct: 1   MSERKILDNGFDEGDKDASNHSINK--VDPRKPAPLTWNRKLNGEGYVPSMFTLTFQEKL 60

Query: 61  HLAPVGYRLFRYIREESVKGRGAMINPFIRRRITDTHAIPLGGLGSGSIGRSYRGEFQRW 120
           H+AP+G RL ++IRE+S KGR   INPF +R IT  H +PLGG+G+GSIGRSY+GEFQRW
Sbjct: 61  HMAPIGIRLLQHIREQSTKGRRVFINPFAKRYITSCHGVPLGGVGAGSIGRSYKGEFQRW 120

Query: 121 QLFPRKCEDKPILANQFSVFVSRPNAEKYSTVLCAQSPETQREVESSGIGSWDWNLKGHS 180
           QLFPR CE+KP+LANQFSVFVSR N EKYS+VLC  SPE  +E   SGIG+WDWNLKG++
Sbjct: 121 QLFPRICEEKPVLANQFSVFVSRSNGEKYSSVLCPASPELLKENAVSGIGTWDWNLKGNN 180

Query: 181 STYHALYPRAWTIYDGEPDPELRIVCRQISPVIPHNYKESSYPVSVFTFTLHNSGKTDAD 240
           STYHALYPRAWT+Y+GEPDPEL+IVCRQISPVIP NYKESS+PVS FTFT++N+GKT AD
Sbjct: 181 STYHALYPRAWTVYEGEPDPELKIVCRQISPVIPDNYKESSFPVSAFTFTVYNTGKTTAD 240

Query: 241 VSLLFTWANSVGGLSEYSGNHINSRTKKKDGVHTVLLHHKTASGFPPVTYAIAAQEGNGV 300
           V+LLFTWANSVGG+SE+SG H NS+   KDGVH +LLHH TA G PPVT+AIAAQE +GV
Sbjct: 241 VTLLFTWANSVGGVSEFSGRHSNSKIMMKDGVHGILLHHMTADGLPPVTFAIAAQETDGV 300

Query: 301 HVSDCPCFVISGNSQGISAKDMWLEIKEHGSFDRLKFADMSMPSEVGSSIGAAVSASVTV 360
           HVS+CPCF+ISGNSQGI+AKDMW EIKEHGSF+ LK  D S+PSE GSSIGAA++AS+ +
Sbjct: 301 HVSECPCFLISGNSQGITAKDMWQEIKEHGSFEHLKSTDASVPSEPGSSIGAAIAASLAI 360

Query: 361 SSDSVRTVTFSLSWDCPEVNFCRGKTYHRRYTKFYGNLGDAAADIARDAILEHHHWESQI 420
            SD+VRTVTFSL+WDCPEV+F  GKTYHRRYTKFYG  GD AA+IA DAIL H HWES I
Sbjct: 361 PSDAVRTVTFSLAWDCPEVDFLGGKTYHRRYTKFYGTDGDVAANIAHDAILGHSHWESLI 420

Query: 421 DAWQRPVLEDKRFPKWYPVTLFNELYYLNAGGTIWTDGSLPIQSLVSIGERGFCLDQCRS 480
           +AWQRP+LEDKR P+WYPVTLFNELYYLN+GGTIWTDGS P+ SLVSIG R F LD+ + 
Sbjct: 421 EAWQRPILEDKRLPEWYPVTLFNELYYLNSGGTIWTDGSPPVHSLVSIGGRKFSLDRSQL 480

Query: 481 G-DSVIYESHENDTANDILGRMTSRLDELRDSVASNSAFGVNLLQKGEENVGQFLYLEGV 540
           G  S+I   H+N TA DILGRMTS L+++   +ASNSAFG NLLQ+GEEN+GQFLYLEG+
Sbjct: 481 GLKSIIDVPHQNGTAIDILGRMTSILEQIHTPIASNSAFGTNLLQEGEENIGQFLYLEGI 540

Query: 541 EYTMWNTYDVHFYSSFAIIMLFPKLELSIQRDFAAAVMMHDPSKMQLLDNGKWEARNVLG 600
           EY MWNTYDVHFY+SFA+IMLFPKL+LSIQRDFAAAVMMHDPSKM+LL +G+W  R VLG
Sbjct: 541 EYHMWNTYDVHFYASFALIMLFPKLQLSIQRDFAAAVMMHDPSKMKLLHDGQWVPRKVLG 600

Query: 601 AVPHDIGVNDPWFEVNGYNLYNTDRWKDLNPKFVLQIYRDVVATGDMKFAKAVWPPVYLA 660
           AVPHDIG++DPWFEVN Y LY+TDRWKDLNPKFVLQ+YRDVVATGD +FA+AVWP VY+A
Sbjct: 601 AVPHDIGIDDPWFEVNAYCLYDTDRWKDLNPKFVLQVYRDVVATGDKRFAEAVWPSVYVA 660

Query: 661 IAYMDQFDRDGDGMIENDGFPDQTYDTWSVSGVSAYSGGLWVAALQAASALARVADEKDA 720
           +AYMDQFD+DGDGMIEN+GFPDQTYDTWSVSGVSAYSGGLWVAALQAASALAR   +K +
Sbjct: 661 MAYMDQFDKDGDGMIENEGFPDQTYDTWSVSGVSAYSGGLWVAALQAASALAREVGDKGS 720

Query: 721 EHYFWFKFQKAKRAYEKLWNGSYFNYDSSGGSSSSSIQADQLAGQWYARASGLLPIVDED 780
           E YFWFKF KAK  Y+KLWNGSYFNYD SG  +SSSIQADQLAGQWYARA GLLPIVDED
Sbjct: 721 EDYFWFKFLKAKAVYQKLWNGSYFNYDDSGSRTSSSIQADQLAGQWYARACGLLPIVDED 780

Query: 781 KAKSALAKVYNYNVMKVKDGKRGAVNGMLPDGTIDFSSMQSREIWSGVTYAVAASMIHED 840
           KA+S L KVYNYNV+KVKDGKRGAVNGMLPDG +D SSMQSREIWSGVTYAVAA+MIHED
Sbjct: 781 KARSTLEKVYNYNVLKVKDGKRGAVNGMLPDGRVDMSSMQSREIWSGVTYAVAATMIHED 840

Query: 841 MTDMAFRTAEGIHEAAWSEDGLGYNFQTPEAWTTTDRYRSLCYMRPLAIWAMQWAFS-EK 900
           + DMAF TA GI EA WSE GLGY+FQTPEAW   D+YRSL YMRPLAIWAMQWA S +K
Sbjct: 841 LVDMAFHTAGGIFEAVWSEKGLGYSFQTPEAWNVDDQYRSLAYMRPLAIWAMQWALSRQK 900

Query: 901 VSVIEELKELDSDAISRHHARFSKVARYLKLPEDGTSSSVLQTVYDYTLKR 950
           +   E   EL +D++  HHA FSKVAR LKLPE+  + S+LQ ++DYT KR
Sbjct: 901 LPKQEPKPELKADSLRIHHAGFSKVARLLKLPEEQGTRSLLQVMFDYTCKR 949

BLAST of Cucsa.023010 vs. TAIR10
Match: AT5G49900.1 (AT5G49900.1 Beta-glucosidase, GBA2 type family protein)

HSP 1 Score: 1332.0 bits (3446), Expect = 0.0e+00
Identity = 635/949 (66.91%), Postives = 759/949 (79.98%), Query Frame = 1

Query: 11  LVEQDEDINKSLTEKTGIDPGQTPSLTWQRKLNREGTSLWQFRLHLKEIIHLAPVGYRLF 70
           +++  ED+    +  T +DP    SLTWQRK++ +  +  +F L +KEI  LAPVG RL+
Sbjct: 6   IMDIGEDVKPLNSSDTKVDPAVPASLTWQRKIDSDVKAPREFNLSVKEIFQLAPVGIRLW 65

Query: 71  RYIREESVKGRGAMINPFIRRRITDTHAIPLGGLGSGSIGRSYRGEFQRWQLFPRKCEDK 130
              REE+ KGR A I+PF +  +T +H +PLGG+G+GSIGRS++GEFQRWQLFP KCED+
Sbjct: 66  FLCREEAAKGRLAFIDPFSKHSVTSSHGVPLGGIGAGSIGRSFKGEFQRWQLFPPKCEDE 125

Query: 131 PILANQFSVFVSRPNAEKYSTVLCAQSPETQREVESSGIGSWDWNLKGHSSTYHALYPRA 190
           P+LANQFS FVSR N +KYS+VLC ++P+  ++   SGIGSWDWNLKG  STYHALYPR+
Sbjct: 126 PVLANQFSAFVSRANGKKYSSVLCPRNPKLDKQDSESGIGSWDWNLKGDKSTYHALYPRS 185

Query: 191 WTIYDGEPDPELRIVCRQISPVIPHNYKESSYPVSVFTFTLHNSGKTDADVSLLFTWANS 250
           WT+Y+GEPDPELRIVCRQ+SP IPHNYKESS+PVSVFTFTLHN G T ADV+LLFTWANS
Sbjct: 186 WTMYEGEPDPELRIVCRQVSPFIPHNYKESSFPVSVFTFTLHNLGNTTADVTLLFTWANS 245

Query: 251 VGGLSEYSGNHINSRTKKKDGVHTVLLHHKTASGFPPVTYAIAAQEGNGVHVSDCPCFVI 310
           VGG SE+SG H NS+    DGV  VLLHHKTA+G P ++YAI+AQ  +GV VS CP F++
Sbjct: 246 VGGDSEFSGGHYNSKITMNDGVQGVLLHHKTANGLPSLSYAISAQATDGVSVSACPFFIV 305

Query: 311 SGNSQGISAKDMWLEIKEHGSFDRLKFADMSMPSEVGSSIGAAVSASVTVSSDSVRTVTF 370
           SG   GI+AKDMW  +KE+GSFD LK ++ SM S+ GSSIGAAV+ASVTV     R VTF
Sbjct: 306 SGKQDGITAKDMWQAVKENGSFDHLKASEASMQSDHGSSIGAAVAASVTVLPGESRIVTF 365

Query: 371 SLSWDCPEVNFCRGKTYHRRYTKFYGNLGDAAADIARDAILEHHHWESQIDAWQRPVLED 430
           SL+WDCPEV F  GK Y RRYTKFYGN GDAAA IA DAIL H  WES I+ WQRP+LED
Sbjct: 366 SLAWDCPEVQFPSGKIYSRRYTKFYGNNGDAAAQIAHDAILGHSQWESWIEDWQRPILED 425

Query: 431 KRFPKWYPVTLFNELYYLNAGGTIWTDGSLPIQSLVSIGERGFCLDQCRSG-DSVIYESH 490
           KR P WYPVTLFNELYYLN+GGT+WTDGS P+ SL  + E+ F LD+ + G  + I   H
Sbjct: 426 KRLPAWYPVTLFNELYYLNSGGTLWTDGSSPVHSLAGVREKKFSLDKSQLGLKNDIDVPH 485

Query: 491 ENDTANDILGRMTSRLDELRDSVASNSAFGVNLLQKGEENVGQFLYLEGVEYTMWNTYDV 550
           +NDTA  +L +M S L+EL  S  SNSAFG  LL++GEEN+G FLYLEG+EY MWNTYDV
Sbjct: 486 QNDTAVSVLEKMASTLEELHASTTSNSAFGTKLLEEGEENIGHFLYLEGIEYRMWNTYDV 545

Query: 551 HFYSSFAIIMLFPKLELSIQRDFAAAVMMHDPSKMQLLDNGKWEARNVLGAVPHDIGVND 610
           HFY+SFA++MLFPKLELSIQRDFAAAVM+HDP+K++ L  G+W  R VLGAVPHD+G+ND
Sbjct: 546 HFYASFALVMLFPKLELSIQRDFAAAVMLHDPTKVKTLSEGQWVQRKVLGAVPHDLGIND 605

Query: 611 PWFEVNGYNLYNTDRWKDLNPKFVLQIYRDVVATGDMKFAKAVWPPVYLAIAYMDQFDRD 670
           PWFEVNGY L+NTDRWKDLNPKFVLQ+YRDVVATGD KFA AVWP VY+A+AYM QFD+D
Sbjct: 606 PWFEVNGYTLHNTDRWKDLNPKFVLQVYRDVVATGDKKFASAVWPSVYVAMAYMAQFDKD 665

Query: 671 GDGMIENDGFPDQTYDTWSVSGVSAYSGGLWVAALQAASALARVADEKDAEHYFWFKFQK 730
           GDGMIEN+GFPDQTYDTWS SGVSAY GGLWVAALQAASALARV  +K+++ YFW KFQK
Sbjct: 666 GDGMIENEGFPDQTYDTWSASGVSAYCGGLWVAALQAASALARVVGDKNSQDYFWSKFQK 725

Query: 731 AKRAYE-KLWNGSYFNYDSSGGSSSSSIQADQLAGQWYARASGLLPIVDEDKAKSALAKV 790
           AK  YE KLWNGSYFNYD+SG   SS+IQADQLAGQWYARASGLLPIVDEDKA++AL KV
Sbjct: 726 AKVVYEKKLWNGSYFNYDNSGSQYSSTIQADQLAGQWYARASGLLPIVDEDKARTALEKV 785

Query: 791 YNYNVMKVKDGKRGAVNGMLPDGTIDFSSMQSREIWSGVTYAVAASMIHEDMTDMAFRTA 850
           YNYNVMK+KDGKRGAVNGM P+G +D +SMQSREIWSGVTYA++A+MI E + +MAF+TA
Sbjct: 786 YNYNVMKIKDGKRGAVNGMHPNGKVDTASMQSREIWSGVTYALSATMIQEGLVEMAFQTA 845

Query: 851 EGIHEAAWSEDGLGYNFQTPEAWTTTDRYRSLCYMRPLAIWAMQWAFSEKVSVIEEL--- 910
            GI+EAAWSE GLGY+FQTPE+W T D YRSL YMRPLAIWAMQWA ++     E+L   
Sbjct: 846 SGIYEAAWSETGLGYSFQTPESWNTVDEYRSLTYMRPLAIWAMQWALTKTSQKQEQLGLE 905

Query: 911 -----KELDSDAISRHHARFSKVARYLKLPEDGTSSSVLQTVYDYTLKR 950
                 EL+  +  +H   FS+V+R L LP + ++ S LQT++DYT +R
Sbjct: 906 PEQQEPELEPSSSMKHDIGFSRVSRLLSLPNEASAKSTLQTLFDYTCRR 954

BLAST of Cucsa.023010 vs. TAIR10
Match: AT1G33700.1 (AT1G33700.1 Beta-glucosidase, GBA2 type family protein)

HSP 1 Score: 1142.1 bits (2953), Expect = 0.0e+00
Identity = 569/951 (59.83%), Postives = 697/951 (73.29%), Query Frame = 1

Query: 16  EDINKSLTEKTGIDPGQTPSLTWQRKLNREGTSLWQFRLHLKEIIHLAPVGYRLFRYIRE 75
           ED NK + +       + P  +W+RKLN +  +  +F+L  ++ +HL P+GYRL+R+ ++
Sbjct: 6   EDQNKMVIDD------KLPPFSWERKLNSQAKTPSEFKLSKRDHMHLFPLGYRLWRHTKD 65

Query: 76  ESVKGRGAMINPFIRRRITDTHAIPLGGLGSGSIGRSYRGEFQRWQLFPRKCEDKPILAN 135
           E+ KGR ++ + F +  IT  H +PLGG+GSGSIGRSY+GEFQ+++LFP+ CE+ PIL N
Sbjct: 66  EAAKGRASIFDIFRKHHITGDHGVPLGGIGSGSIGRSYKGEFQQFKLFPKICEEAPILTN 125

Query: 136 QFSVFVSRPNAEKYSTVLCAQSPET---------QREVESSGIGSWDWNLKGHSSTYHAL 195
           QFS FVSRP   K+STVLC   P+          Q +  + GI SWDWN+ G  STYHAL
Sbjct: 126 QFSAFVSRPGGVKHSTVLCPSKPQVVKDNGGYLCQGQAPNMGIESWDWNMTGEKSTYHAL 185

Query: 196 YPRAWTIYDGEPDPELRIVCRQISPVIPHNYKESSYPVSVFTFTLHNSGKTDADVSLLFT 255
           YPR+WT+YDGEPDPELRIV RQ+SP IPHNY+ESS PVSVF FT+ N+G   A V+LLFT
Sbjct: 186 YPRSWTVYDGEPDPELRIVSRQVSPFIPHNYEESSLPVSVFNFTVTNTGAEPAIVTLLFT 245

Query: 256 WANSVGGLSEYSGNHINSRTKKKDGVHTVLLHHKTASGFPPVTYAIAAQEGNGVHVSDCP 315
           W NSVGG S  +G H NS  K KDGVH V L HKTA+G PPV+YAIAA+E   V VS CP
Sbjct: 246 WENSVGGASGLTGQHFNSTMKAKDGVHAVALQHKTANGHPPVSYAIAAKETEDVRVSSCP 305

Query: 316 CFVISGNSQG-ISAKDMWLEIKEHGSFDRLKFADMSMPSEVGSSIGAAVSASVTVSSDSV 375
           CF++SG +   I+A DMW EIK++ SFD+L  ++   PS+ G+SIGAA++A V V     
Sbjct: 306 CFIVSGTTPNQITAGDMWDEIKKNASFDKLT-SNACSPSKPGTSIGAAIAAKVKVPPGCD 365

Query: 376 RTVTFSLSWDCPEVNFCRGKTYHRRYTKFYGNLGDAAADIARDAILEHHHWESQIDAWQR 435
           RTVTFSLSWDCPE  F   KTYHRRYT+FYG+LG+AA  +A DA+L    WE+QI+ WQ 
Sbjct: 366 RTVTFSLSWDCPEARFDE-KTYHRRYTRFYGSLGNAAVAMAHDALLNFPEWETQIEEWQA 425

Query: 436 PVLEDKRFPKWYPVTLFNELYYLNAGGTIWTDGSLPIQSLVSIGERGFCLDQCRSGDSVI 495
           PVL D   P+WY VTLFNELYY N+GGT+WTDG  P QSL SIG R   L     G S I
Sbjct: 426 PVLADTTLPEWYRVTLFNELYYFNSGGTMWTDGLPPKQSLDSIGRRKISL-----GLSTI 485

Query: 496 --YESHENDTANDILGRMTSRLDELRDSVASNSAFGVNLLQKGEENVGQFLYLEGVEYTM 555
              +  +N+ A DILGR+ +   ++   + SN+A G  ++Q   EN+GQFLYLEGV+Y M
Sbjct: 486 DKNDQDQNNVALDILGRIDAVCSQIHAPLTSNAALGNTMIQNTTENIGQFLYLEGVQYLM 545

Query: 556 WNTYDVHFYSSFAIIMLFPKLELSIQRDFAAAVMMHDPSKMQLLDNGKWEARNVLGAVPH 615
           +NTYDVHFYSSFA++MLFPK+ELSIQRDFAAAV+MHD SK Q++ +G++  R VLGAVPH
Sbjct: 546 YNTYDVHFYSSFALLMLFPKIELSIQRDFAAAVLMHDSSKKQVMSSGEFVTRKVLGAVPH 605

Query: 616 DIGVNDPWFEVNGYNLYNTDRWKDLNPKFVLQIYRDVVATGDMKFAKAVWPPVYLAIAYM 675
           DIG+NDPWFE+N YNL+NTDRWKDLN KFVLQ+YRDVVATGD+ FAKAVWP VY AIAY+
Sbjct: 606 DIGLNDPWFELNAYNLFNTDRWKDLNSKFVLQVYRDVVATGDLNFAKAVWPSVYTAIAYL 665

Query: 676 DQFDRDGDGMIENDGFPDQTYDTWSVSGVSAYSGGLWVAALQAASALARVADEKDAEHYF 735
           DQFD+DGDGMIEN+GFPDQTYD WS SGVSAY GGLWVAALQA SALAR   +  A  YF
Sbjct: 666 DQFDKDGDGMIENEGFPDQTYDAWSCSGVSAYCGGLWVAALQAGSALAREIGDNGAAVYF 725

Query: 736 WFKFQKAKRAYEKLWNGSYFNYDSSGGSSSSSIQADQLAGQWYARASGLLPIVDEDKAKS 795
             K++KA+  YEKLWNGSYFNYD+S   SSSSI ADQ+AGQWYARA GL PI  E+  K 
Sbjct: 726 NAKYEKARSVYEKLWNGSYFNYDNSRSGSSSSILADQMAGQWYARACGLKPIAKEEWIKK 785

Query: 796 ALAKVYNYNVMKVKDGKRGAVNGMLPDGTIDFSSMQSREIWSGVTYAVAASMIHEDMTDM 855
           AL  VY++NVM+V+DG RGAVNGMLPDG +D S+M SRE+W+G TY+VAA MI E + D 
Sbjct: 786 ALETVYDFNVMRVRDGTRGAVNGMLPDGRVDTSTMVSREVWAGTTYSVAACMIQEGLADK 845

Query: 856 AFRTAEGIHEAAWSEDGLGYNFQTPEAWTTTDRYRSLCYMRPLAIWAMQWAFSEKVSVIE 915
            FRTA GI+EAAWS+ GLG  FQTPEAWTT D YRSLCYMRPLAIW +QWA +      E
Sbjct: 846 GFRTASGIYEAAWSDRGLGCAFQTPEAWTTNDEYRSLCYMRPLAIWGIQWAHTMPKPNRE 905

Query: 916 E---LKELDSDAIS---RHHARFSKVARYLKLPEDGTSSSVLQTVYDYTLK 949
           +   L+  + DA S   + HA F KVA YLK  +     + LQT Y+  LK
Sbjct: 906 QEVSLRPQEEDATSVLFQQHAGFIKVAHYLKNTKGKDHRNRLQTAYETFLK 943

BLAST of Cucsa.023010 vs. TAIR10
Match: AT4G10060.1 (AT4G10060.1 Beta-glucosidase, GBA2 type family protein)

HSP 1 Score: 1109.0 bits (2867), Expect = 0.0e+00
Identity = 543/925 (58.70%), Postives = 677/925 (73.19%), Query Frame = 1

Query: 34  PSLTWQRKLNREGTSLWQFRLHLKEIIHLAPVGYRLFRYIREESVKGRGAMINPFIRRRI 93
           P +TWQRKLN +  +  +F++ +++++HL P+GYRL+RY +EE+ KGR +M + F +R +
Sbjct: 22  PQVTWQRKLNSKVKNPSEFKMSIRDVLHLFPLGYRLWRYTKEEAKKGRFSMYDIFKKRHV 81

Query: 94  TDTHAIPLGGLGSGSIGRSYRGEFQRWQLFPRKCEDKPILANQFSVFVSRPNAEKYSTVL 153
              H +PLGG+G GSIGRSY+GEFQ+++LFP+ CE+ PIL NQFSVFVSRP    YSTVL
Sbjct: 82  RGDHGVPLGGIGGGSIGRSYKGEFQQFKLFPKICEEAPILTNQFSVFVSRPGGLSYSTVL 141

Query: 154 CAQSPETQR-EVESSGIGSWDWNLKGHSSTYHALYPRAWTIYDGEPDPELRIVCRQISPV 213
           C   P++ + + E  GI SWDWN++G  STYHALYPR+WT+Y+ EPDPELRIV RQ+SP 
Sbjct: 142 CPTKPKSVKGKTEDLGIESWDWNMEGDKSTYHALYPRSWTVYN-EPDPELRIVSRQVSPF 201

Query: 214 IPHNYKESSYPVSVFTFTLHNSGKTDADVSLLFTWANSVGGLSEYSGNHINSRTKKKDGV 273
           IPHNYKESS PVSVF FT+ N GK +A V+LLFTW NSVGG S  +G H NS   ++DGV
Sbjct: 202 IPHNYKESSLPVSVFEFTMSNLGKEEATVTLLFTWENSVGGASGLTGEHFNSTIMERDGV 261

Query: 274 HTVLLHHKTASGFPPVTYAIAAQEGNGVHVSDCPCFVISGNS-QGISAKDMWLEIKEHGS 333
           H ++LHHKT +G PPVTYAIAAQE   VHVS+CPCF++SG+S + I+AK+MW EIK++ S
Sbjct: 262 HAIVLHHKTGNGHPPVTYAIAAQETEDVHVSECPCFLVSGHSPKEITAKEMWDEIKKNKS 321

Query: 334 FDRLKFADMSMPSEVGSSIGAAVSASVTVSSDSVRTVTFSLSWDCPEVNFCRGKTYHRRY 393
           FD L  ++   PS  G+SIGAA++A V V     RTVTFSLSWDCPEV F   KTYHRRY
Sbjct: 322 FDELN-SEPGSPSRPGTSIGAAIAAKVKVPPGCDRTVTFSLSWDCPEVRF-NEKTYHRRY 381

Query: 394 TKFYGNLGDAAADIARDAILEHHHWESQIDAWQRPVLEDKRFPKWYPVTLFNELYYLNAG 453
           TKFYGNLGDAA  +ARDA+L +  WESQI+AWQ P+L D   P WY VTLFNELYY N+G
Sbjct: 382 TKFYGNLGDAAVKMARDALLNYVDWESQIEAWQSPILSDTTLPDWYRVTLFNELYYFNSG 441

Query: 454 GTIWTDGSLPIQSLVSIGERGFCLDQCRSGDSVIYESHENDTANDILGRMTSRLDELRDS 513
           GTIWTDG  P +S+    ER           S +  + +ND   D+  ++ +  +++   
Sbjct: 442 GTIWTDGLPPKESI----ER-----------SKVTNTEQNDIVIDLFQKINAVCEQIYSP 501

Query: 514 VASNSAFGVNLLQKGEENVGQFLYLEGVEYTMWNTYDVHFYSSFAIIMLFPKLELSIQRD 573
            +SNS          EEN+GQF+YLEG+EY M+NTYDVHFYSSFA++ LFPKL LSIQRD
Sbjct: 502 QSSNS----------EENIGQFIYLEGIEYLMYNTYDVHFYSSFALLSLFPKLALSIQRD 561

Query: 574 FAAAVMMHDPSKMQLLDNGKWEARNVLGAVPHDIGVNDPWFEVNGYNLYNTDRWKDLNPK 633
           FAA V++ DP+K +++ +G+W  R +LG+VPHDIG+NDPW E+N YN +NTDRWKDLN K
Sbjct: 562 FAATVLIQDPTKKKIMSSGEWVTRKLLGSVPHDIGLNDPWLELNEYNFFNTDRWKDLNAK 621

Query: 634 FVLQIYRDVVATGDMKFAKAVWPPVYLAIAYMDQFDRDGDGMIENDGFPDQTYDTWSVSG 693
           FVLQ+YRDVVAT D  FAKAVWP VY A+AY+DQFD+D DGMIEN+GFPDQTYD WSV+G
Sbjct: 622 FVLQVYRDVVATNDQSFAKAVWPSVYTAVAYLDQFDKDEDGMIENEGFPDQTYDAWSVTG 681

Query: 694 VSAYSGGLWVAALQAASALARVADEKDAEHYFWFKFQKAKRAYEKLWNGSYFNYDSSGGS 753
           VSAY GGLWVAALQAASA A +  E     YF  K++KAK  YEKLWNGSYFNYD SG  
Sbjct: 682 VSAYCGGLWVAALQAASAFASIVGENAVAIYFNAKYEKAKIVYEKLWNGSYFNYDDSGSG 741

Query: 754 SSSSIQADQLAGQWYARASGLLPIVDEDKAKSALAKVYNYNVMKVKDGKRGAVNGMLPDG 813
           SSSSI ADQLAGQWYARA GL PI  E+  K AL  +Y +NVMKVK G RGAVNGM  +G
Sbjct: 742 SSSSILADQLAGQWYARACGLKPITKEEWIKKALETIYEFNVMKVKGGTRGAVNGMSTEG 801

Query: 814 TIDFSSMQSREIWSGVTYAVAASMIHEDMTDMAFRTAEGIHEAAWSEDGLGYNFQTPEAW 873
            +D +S+ S+E+W+G TY+VAA MI E   +  F+TA GI+EA WS+ GL  +FQTPEAW
Sbjct: 802 KVDTNSLVSKEVWAGTTYSVAACMIQEGQREKGFQTASGIYEAIWSDRGLSCSFQTPEAW 861

Query: 874 TTTDRYRSLCYMRPLAIWAMQWAFSEKVSVIEELKEL-------DSDAISRHHARFSKVA 933
              D YRSLCYMRPLAIWA+QWA +   S  EE ++L       +S+ + R H  F  VA
Sbjct: 862 NMNDEYRSLCYMRPLAIWAIQWALTRTQSFGEEKQKLVAGDEEEESNLLLRQHKGFKDVA 918

Query: 934 RYLKL-PEDGTSSSVLQTVYDYTLK 949
           R++K+ P      S LQ  Y+  LK
Sbjct: 922 RFVKIVPTSNVHRSRLQHTYETVLK 918

BLAST of Cucsa.023010 vs. TAIR10
Match: AT3G24180.1 (AT3G24180.1 Beta-glucosidase, GBA2 type family protein)

HSP 1 Score: 934.1 bits (2413), Expect = 6.8e-272
Identity = 479/925 (51.78%), Postives = 613/925 (66.27%), Query Frame = 1

Query: 15  DEDINKSLTEKTGIDPGQTPSLTWQRKLNREGTSLWQFRLHLKEIIHLAPVGYRLFRYIR 74
           +E I++S  +    D    P   W+R+LN     L +F +  +E I +  +G RL+ Y+R
Sbjct: 17  EEFISRSTLQLLDFDSAAPPPHAWRRRLNCHANILKEFTITFREAIKMVRLGIRLWSYVR 76

Query: 75  EESVKGRGAMINPFIRRRI--TDTHAIPLGGLGSGSIGRSYRGEFQRWQLFPRKCEDKPI 134
           EE+  GR A I+PF +     + +  +PLGG+GSGSI R +RGEF++WQ+ P  C+  P+
Sbjct: 77  EEASHGRKAPIDPFTKENCKPSASQGVPLGGMGSGSISRGFRGEFKQWQITPGTCDPSPM 136

Query: 135 LANQFSVFVSRPNA-EKYSTVLCAQSPETQREVESSGIGSWDWNLKGHSSTYHALYPRAW 194
           ++NQFS+F+SR    +KY++VL      +  +    G+ SW WNL G  STYHAL+PRAW
Sbjct: 137 MSNQFSIFISRDGGHKKYASVLSPGQHGSLGKSRDKGLSSWGWNLNGQHSTYHALFPRAW 196

Query: 195 TIYDGEPDPELRIVCRQISPVIPHNYKESSYPVSVFTFTLHNSGKTDADVSLLFTWANSV 254
           TIYDGEPDPEL+I CRQISP IP+NY++SS P +VF +TL N+GK  A VSLLFTWANS+
Sbjct: 197 TIYDGEPDPELKISCRQISPFIPNNYRDSSLPAAVFVYTLVNTGKERAKVSLLFTWANSM 256

Query: 255 GGLSEYSGNHINSRTKKKDGVHTVLLHHKTASGFPPVTYAIAAQEGNGVHVSDCPCFVIS 314
           GG S  SG H+N     +DGV  VLLHHKT  G PPVT+AIAA E   V+V+  PCF +S
Sbjct: 257 GGTSHMSGGHVNEPFIGEDGVSGVLLHHKTGKGNPPVTFAIAASETQNVNVTVLPCFGLS 316

Query: 315 GNSQGISAKDMWLEIKEHGSFDRLKF-ADMSMPSEVGSSIGAAVSASVTVSSDSVRTVTF 374
            +S   +AKDMW  +++ G FD+  F +  S PS  G +I AAVSAS  V +    TV+F
Sbjct: 317 EDSS-FTAKDMWDTMEQDGKFDQENFNSGPSTPSLAGDTICAAVSASAWVEAHGKCTVSF 376

Query: 375 SLSWDCPEVNFCRGKTYHRRYTKFYGNLGDAAADIARDAILEHHHWESQIDAWQRPVLED 434
           +LSW  P+V F +G TY RRYTKFYG    AA D+  DA+  +  WE  I+AWQ P+L D
Sbjct: 377 ALSWSSPKVKFSKGSTYDRRYTKFYGTSPRAALDLVHDALTNYKRWEEDIEAWQNPILRD 436

Query: 435 KRFPKWYPVTLFNELYYLNAGGTIWTDGSLPIQSLVSIGERGFCLDQCRSGDSVIYESHE 494
           +R P+WY  TLFNELY+L AGGT+W D S    SL + G          + D  +     
Sbjct: 437 ERLPEWYKFTLFNELYFLVAGGTVWIDSS----SLNANGNSQHQQSGLGNSDGKVGGLDI 496

Query: 495 NDTANDILGRMTSRLDELRDSVAS----NSAFGVNLLQKGEENVGQFLYLEGVEYTMWNT 554
           ND  ND LG   S   +  D V++    N  F         ++VG+FLYLEGVEY MW T
Sbjct: 497 NDQRND-LGNGNSVGVKSNDEVSAIHNRNGLFVDTPHVDDGDDVGRFLYLEGVEYVMWCT 556

Query: 555 YDVHFYSSFAIIMLFPKLELSIQRDFAAAVMMHDPSKMQLLDNGKWEARNVLGAVPHDIG 614
           YDVHFY+S+A++MLFPK+EL+IQRDFA AV+  D  K++ L  G    R V GAVPHD+G
Sbjct: 557 YDVHFYASYALLMLFPKIELNIQRDFAKAVLSEDGRKVKFLAEGNVGIRKVRGAVPHDLG 616

Query: 615 VNDPWFEVNGYNLYNTDRWKDLNPKFVLQIYRDVVATGDMKFAKAVWPPVYLAIAYMDQF 674
           ++DPW E+N YN+++T RWKDLNPKFVLQ+YRD  ATGD +F   VWP V  A+ YM+QF
Sbjct: 617 MHDPWNEMNAYNIHDTSRWKDLNPKFVLQVYRDFAATGDYQFGIDVWPAVRAAMEYMEQF 676

Query: 675 DRDGDGMIENDGFPDQTYDTWSVSGVSAYSGGLWVAALQAASALARVADEKDAEHYFWFK 734
           DRD D +IENDGFPDQTYDTW+V GVSAY G LW+AALQAA+A+A    +K        K
Sbjct: 677 DRDNDDLIENDGFPDQTYDTWTVHGVSAYCGCLWLAALQAAAAMALQIGDKFFAELCKNK 736

Query: 735 FQKAKRAYE-KLWNGSYFNYDSSGGSSSSSIQADQLAGQWYARASGLLPIVDEDKAKSAL 794
           F  AK A E KLWNGSYFNYDS   S+S SIQ DQLAGQWYA +SGL P+ +E K +S +
Sbjct: 737 FLNAKAALETKLWNGSYFNYDSGSSSNSKSIQTDQLAGQWYAASSGLPPLFEESKIRSTM 796

Query: 795 AKVYNYNVMKVKDGKRGAVNGMLPDGTIDFSSMQSREIWSGVTYAVAASMIHEDMTDMAF 854
            K++++NVMK K GK GAVNGM PDG +D + MQSREIW+GVTYA AA+MI   M +  F
Sbjct: 797 QKIFDFNVMKTKGGKMGAVNGMHPDGKVDDTCMQSREIWTGVTYAAAATMILSGMEEQGF 856

Query: 855 RTAEGIHEAAWSEDGLGYNFQTPEAWTTTDRYRSLCYMRPLAIWAMQWAFSEKVSVIE-- 914
            TAEGI  A WSE+G GY FQTPE WT    YRSL YMRPLAIW MQWA S   ++++  
Sbjct: 857 TTAEGIFTAGWSEEGFGYWFQTPEGWTMDGHYRSLIYMRPLAIWGMQWALSLPKAILDAP 916

Query: 915 ELKELDSDAISRHHARFSKVARYLK 929
           ++  +D   +S    RFS   + +K
Sbjct: 917 QINMMDRVHLSPRSRRFSNNFKVVK 935

BLAST of Cucsa.023010 vs. NCBI nr
Match: gi|449463809|ref|XP_004149624.1| (PREDICTED: non-lysosomal glucosylceramidase [Cucumis sativus])

HSP 1 Score: 1959.5 bits (5075), Expect = 0.0e+00
Identity = 951/951 (100.00%), Postives = 951/951 (100.00%), Query Frame = 1

Query: 1   MSEGKKLGNGLVEQDEDINKSLTEKTGIDPGQTPSLTWQRKLNREGTSLWQFRLHLKEII 60
           MSEGKKLGNGLVEQDEDINKSLTEKTGIDPGQTPSLTWQRKLNREGTSLWQFRLHLKEII
Sbjct: 1   MSEGKKLGNGLVEQDEDINKSLTEKTGIDPGQTPSLTWQRKLNREGTSLWQFRLHLKEII 60

Query: 61  HLAPVGYRLFRYIREESVKGRGAMINPFIRRRITDTHAIPLGGLGSGSIGRSYRGEFQRW 120
           HLAPVGYRLFRYIREESVKGRGAMINPFIRRRITDTHAIPLGGLGSGSIGRSYRGEFQRW
Sbjct: 61  HLAPVGYRLFRYIREESVKGRGAMINPFIRRRITDTHAIPLGGLGSGSIGRSYRGEFQRW 120

Query: 121 QLFPRKCEDKPILANQFSVFVSRPNAEKYSTVLCAQSPETQREVESSGIGSWDWNLKGHS 180
           QLFPRKCEDKPILANQFSVFVSRPNAEKYSTVLCAQSPETQREVESSGIGSWDWNLKGHS
Sbjct: 121 QLFPRKCEDKPILANQFSVFVSRPNAEKYSTVLCAQSPETQREVESSGIGSWDWNLKGHS 180

Query: 181 STYHALYPRAWTIYDGEPDPELRIVCRQISPVIPHNYKESSYPVSVFTFTLHNSGKTDAD 240
           STYHALYPRAWTIYDGEPDPELRIVCRQISPVIPHNYKESSYPVSVFTFTLHNSGKTDAD
Sbjct: 181 STYHALYPRAWTIYDGEPDPELRIVCRQISPVIPHNYKESSYPVSVFTFTLHNSGKTDAD 240

Query: 241 VSLLFTWANSVGGLSEYSGNHINSRTKKKDGVHTVLLHHKTASGFPPVTYAIAAQEGNGV 300
           VSLLFTWANSVGGLSEYSGNHINSRTKKKDGVHTVLLHHKTASGFPPVTYAIAAQEGNGV
Sbjct: 241 VSLLFTWANSVGGLSEYSGNHINSRTKKKDGVHTVLLHHKTASGFPPVTYAIAAQEGNGV 300

Query: 301 HVSDCPCFVISGNSQGISAKDMWLEIKEHGSFDRLKFADMSMPSEVGSSIGAAVSASVTV 360
           HVSDCPCFVISGNSQGISAKDMWLEIKEHGSFDRLKFADMSMPSEVGSSIGAAVSASVTV
Sbjct: 301 HVSDCPCFVISGNSQGISAKDMWLEIKEHGSFDRLKFADMSMPSEVGSSIGAAVSASVTV 360

Query: 361 SSDSVRTVTFSLSWDCPEVNFCRGKTYHRRYTKFYGNLGDAAADIARDAILEHHHWESQI 420
           SSDSVRTVTFSLSWDCPEVNFCRGKTYHRRYTKFYGNLGDAAADIARDAILEHHHWESQI
Sbjct: 361 SSDSVRTVTFSLSWDCPEVNFCRGKTYHRRYTKFYGNLGDAAADIARDAILEHHHWESQI 420

Query: 421 DAWQRPVLEDKRFPKWYPVTLFNELYYLNAGGTIWTDGSLPIQSLVSIGERGFCLDQCRS 480
           DAWQRPVLEDKRFPKWYPVTLFNELYYLNAGGTIWTDGSLPIQSLVSIGERGFCLDQCRS
Sbjct: 421 DAWQRPVLEDKRFPKWYPVTLFNELYYLNAGGTIWTDGSLPIQSLVSIGERGFCLDQCRS 480

Query: 481 GDSVIYESHENDTANDILGRMTSRLDELRDSVASNSAFGVNLLQKGEENVGQFLYLEGVE 540
           GDSVIYESHENDTANDILGRMTSRLDELRDSVASNSAFGVNLLQKGEENVGQFLYLEGVE
Sbjct: 481 GDSVIYESHENDTANDILGRMTSRLDELRDSVASNSAFGVNLLQKGEENVGQFLYLEGVE 540

Query: 541 YTMWNTYDVHFYSSFAIIMLFPKLELSIQRDFAAAVMMHDPSKMQLLDNGKWEARNVLGA 600
           YTMWNTYDVHFYSSFAIIMLFPKLELSIQRDFAAAVMMHDPSKMQLLDNGKWEARNVLGA
Sbjct: 541 YTMWNTYDVHFYSSFAIIMLFPKLELSIQRDFAAAVMMHDPSKMQLLDNGKWEARNVLGA 600

Query: 601 VPHDIGVNDPWFEVNGYNLYNTDRWKDLNPKFVLQIYRDVVATGDMKFAKAVWPPVYLAI 660
           VPHDIGVNDPWFEVNGYNLYNTDRWKDLNPKFVLQIYRDVVATGDMKFAKAVWPPVYLAI
Sbjct: 601 VPHDIGVNDPWFEVNGYNLYNTDRWKDLNPKFVLQIYRDVVATGDMKFAKAVWPPVYLAI 660

Query: 661 AYMDQFDRDGDGMIENDGFPDQTYDTWSVSGVSAYSGGLWVAALQAASALARVADEKDAE 720
           AYMDQFDRDGDGMIENDGFPDQTYDTWSVSGVSAYSGGLWVAALQAASALARVADEKDAE
Sbjct: 661 AYMDQFDRDGDGMIENDGFPDQTYDTWSVSGVSAYSGGLWVAALQAASALARVADEKDAE 720

Query: 721 HYFWFKFQKAKRAYEKLWNGSYFNYDSSGGSSSSSIQADQLAGQWYARASGLLPIVDEDK 780
           HYFWFKFQKAKRAYEKLWNGSYFNYDSSGGSSSSSIQADQLAGQWYARASGLLPIVDEDK
Sbjct: 721 HYFWFKFQKAKRAYEKLWNGSYFNYDSSGGSSSSSIQADQLAGQWYARASGLLPIVDEDK 780

Query: 781 AKSALAKVYNYNVMKVKDGKRGAVNGMLPDGTIDFSSMQSREIWSGVTYAVAASMIHEDM 840
           AKSALAKVYNYNVMKVKDGKRGAVNGMLPDGTIDFSSMQSREIWSGVTYAVAASMIHEDM
Sbjct: 781 AKSALAKVYNYNVMKVKDGKRGAVNGMLPDGTIDFSSMQSREIWSGVTYAVAASMIHEDM 840

Query: 841 TDMAFRTAEGIHEAAWSEDGLGYNFQTPEAWTTTDRYRSLCYMRPLAIWAMQWAFSEKVS 900
           TDMAFRTAEGIHEAAWSEDGLGYNFQTPEAWTTTDRYRSLCYMRPLAIWAMQWAFSEKVS
Sbjct: 841 TDMAFRTAEGIHEAAWSEDGLGYNFQTPEAWTTTDRYRSLCYMRPLAIWAMQWAFSEKVS 900

Query: 901 VIEELKELDSDAISRHHARFSKVARYLKLPEDGTSSSVLQTVYDYTLKRFF 952
           VIEELKELDSDAISRHHARFSKVARYLKLPEDGTSSSVLQTVYDYTLKRFF
Sbjct: 901 VIEELKELDSDAISRHHARFSKVARYLKLPEDGTSSSVLQTVYDYTLKRFF 951

BLAST of Cucsa.023010 vs. NCBI nr
Match: gi|659129612|ref|XP_008464753.1| (PREDICTED: non-lysosomal glucosylceramidase-like isoform X1 [Cucumis melo])

HSP 1 Score: 1911.7 bits (4951), Expect = 0.0e+00
Identity = 924/952 (97.06%), Postives = 939/952 (98.63%), Query Frame = 1

Query: 1   MSEGKKLGNGLVEQDEDINKSLTEKTGIDPGQTPSLTWQRKLNREGTSLWQFRLHLKEII 60
           MSEGKKL NG VEQDEDINKSLTEKTG DPGQTPSLTWQRKLNREG SLWQFRLHLKEII
Sbjct: 1   MSEGKKLENGFVEQDEDINKSLTEKTGTDPGQTPSLTWQRKLNREGPSLWQFRLHLKEII 60

Query: 61  HLAPVGYRLFRYIREESVKGRGAMINPFIRRRITDTHAIPLGGLGSGSIGRSYRGEFQRW 120
           HLAPVG+RL RYIREESVKGRGAMINPFIRRRITDTHA+PLGGLGSGSIGRSYRGEFQRW
Sbjct: 61  HLAPVGFRLIRYIREESVKGRGAMINPFIRRRITDTHAVPLGGLGSGSIGRSYRGEFQRW 120

Query: 121 QLFPRKCEDKPILANQFSVFVSRPNAEKYSTVLCAQSPETQREVESSGIGSWDWNLKGHS 180
           QLFPRKCEDKPILANQFSVFVSRPN EKYSTVLCAQSPE QREVESSGIGSWDWNLKGHS
Sbjct: 121 QLFPRKCEDKPILANQFSVFVSRPNGEKYSTVLCAQSPENQREVESSGIGSWDWNLKGHS 180

Query: 181 STYHALYPRAWTIYDGEPDPELRIVCRQISPVIPHNYKESSYPVSVFTFTLHNSGKTDAD 240
           STYHALYPRAWTIY+GEPDPELRIVCRQISPVIPHNYKESSYPVSVFTFTLHNSGKTDAD
Sbjct: 181 STYHALYPRAWTIYNGEPDPELRIVCRQISPVIPHNYKESSYPVSVFTFTLHNSGKTDAD 240

Query: 241 VSLLFTWANSVGGLSEYSGNHINSRTKKKDGVHTVLLHHKTASGFPPVTYAIAAQEGNGV 300
           V+LLFTWANSVGGLSEYSGNHINSRTKKKDGVHTVLLHHKTASG+PPVTYAIAAQEGNGV
Sbjct: 241 VNLLFTWANSVGGLSEYSGNHINSRTKKKDGVHTVLLHHKTASGYPPVTYAIAAQEGNGV 300

Query: 301 HVSDCPCFVISGNSQGISAKDMWLEIKEHGSFDRLKFADMSMPSEVGSSIGAAVSASVTV 360
           HVSDCPCFVISG+SQGISAKDMWLEIKEHGSFDRLKFADMSMPSEVGSSIGAA++AS+TV
Sbjct: 301 HVSDCPCFVISGHSQGISAKDMWLEIKEHGSFDRLKFADMSMPSEVGSSIGAAITASITV 360

Query: 361 SSDSVRTVTFSLSWDCPEVNFCRGKTYHRRYTKFYGNLGDAAADIARDAILEHHHWESQI 420
           SSDSVRTVTFSLSWDCPEVNFCRGKTYHRRYTKFYGNLGDAAADIARDAILEHHHWESQI
Sbjct: 361 SSDSVRTVTFSLSWDCPEVNFCRGKTYHRRYTKFYGNLGDAAADIARDAILEHHHWESQI 420

Query: 421 DAWQRPVLEDKRFPKWYPVTLFNELYYLNAGGTIWTDGSLPIQSLVSIGERGFCLDQCRS 480
           DAWQRPVLEDKRFPKWYPVTLFNELYYLNAGGTIWTDGSLPIQSLVSIGERGFCLDQCRS
Sbjct: 421 DAWQRPVLEDKRFPKWYPVTLFNELYYLNAGGTIWTDGSLPIQSLVSIGERGFCLDQCRS 480

Query: 481 G-DSVIYESHENDTANDILGRMTSRLDELRDSVASNSAFGVNLLQKGEENVGQFLYLEGV 540
           G  SVIYESHENDTANDILGRMTSRLDELRDSV SNSAFGVNLLQKGEENVGQFLYLEGV
Sbjct: 481 GLKSVIYESHENDTANDILGRMTSRLDELRDSVESNSAFGVNLLQKGEENVGQFLYLEGV 540

Query: 541 EYTMWNTYDVHFYSSFAIIMLFPKLELSIQRDFAAAVMMHDPSKMQLLDNGKWEARNVLG 600
           EYTMWNTYDVHFYSSFAIIMLFPKLELSIQRDFAAAVMMHDPSKMQLLDNGKWEARNVLG
Sbjct: 541 EYTMWNTYDVHFYSSFAIIMLFPKLELSIQRDFAAAVMMHDPSKMQLLDNGKWEARNVLG 600

Query: 601 AVPHDIGVNDPWFEVNGYNLYNTDRWKDLNPKFVLQIYRDVVATGDMKFAKAVWPPVYLA 660
           AVPHDIGVNDPWFEVNGYNLYNTDRWKDLNPKFVLQIYRDVVATGDMKFAKAVWPPVYL+
Sbjct: 601 AVPHDIGVNDPWFEVNGYNLYNTDRWKDLNPKFVLQIYRDVVATGDMKFAKAVWPPVYLS 660

Query: 661 IAYMDQFDRDGDGMIENDGFPDQTYDTWSVSGVSAYSGGLWVAALQAASALARVADEKDA 720
           IAYMDQFDRDGDGMIENDGFPDQTYDTWSV+GVSAYSGGLWVAALQAASALARV DEKDA
Sbjct: 661 IAYMDQFDRDGDGMIENDGFPDQTYDTWSVTGVSAYSGGLWVAALQAASALARVVDEKDA 720

Query: 721 EHYFWFKFQKAKRAYEKLWNGSYFNYDSSGGSSSSSIQADQLAGQWYARASGLLPIVDED 780
           EHYFWFKFQKAKRAYEKLWNGSYFNYDSSGGSSSSSIQADQLAGQWYARASGLLPIVDED
Sbjct: 721 EHYFWFKFQKAKRAYEKLWNGSYFNYDSSGGSSSSSIQADQLAGQWYARASGLLPIVDED 780

Query: 781 KAKSALAKVYNYNVMKVKDGKRGAVNGMLPDGTIDFSSMQSREIWSGVTYAVAASMIHED 840
           KAKSALAKVYNYNVMKVKDGKRGAVNGMLPDGT+DFSSMQSREIWSGVTYAVAASMIHE+
Sbjct: 781 KAKSALAKVYNYNVMKVKDGKRGAVNGMLPDGTVDFSSMQSREIWSGVTYAVAASMIHEN 840

Query: 841 MTDMAFRTAEGIHEAAWSEDGLGYNFQTPEAWTTTDRYRSLCYMRPLAIWAMQWAFSEKV 900
           MTDMAFRTA+GIHEAAWSEDGLGYNFQTPEAWTTTDRYRSLCYMRPLAIWAMQWAFSEKV
Sbjct: 841 MTDMAFRTAQGIHEAAWSEDGLGYNFQTPEAWTTTDRYRSLCYMRPLAIWAMQWAFSEKV 900

Query: 901 SVIEELKELDSDAISRHHARFSKVARYLKLPEDGTSSSVLQTVYDYTLKRFF 952
           SVIEELKELDSDAI RHHA+FSKVAR LKLPEDGTSSSVLQTVYDYTLKRFF
Sbjct: 901 SVIEELKELDSDAILRHHAKFSKVARLLKLPEDGTSSSVLQTVYDYTLKRFF 952

BLAST of Cucsa.023010 vs. NCBI nr
Match: gi|659129614|ref|XP_008464754.1| (PREDICTED: non-lysosomal glucosylceramidase-like isoform X2 [Cucumis melo])

HSP 1 Score: 1756.9 bits (4549), Expect = 0.0e+00
Identity = 847/869 (97.47%), Postives = 861/869 (99.08%), Query Frame = 1

Query: 84  MINPFIRRRITDTHAIPLGGLGSGSIGRSYRGEFQRWQLFPRKCEDKPILANQFSVFVSR 143
           MINPFIRRRITDTHA+PLGGLGSGSIGRSYRGEFQRWQLFPRKCEDKPILANQFSVFVSR
Sbjct: 1   MINPFIRRRITDTHAVPLGGLGSGSIGRSYRGEFQRWQLFPRKCEDKPILANQFSVFVSR 60

Query: 144 PNAEKYSTVLCAQSPETQREVESSGIGSWDWNLKGHSSTYHALYPRAWTIYDGEPDPELR 203
           PN EKYSTVLCAQSPE QREVESSGIGSWDWNLKGHSSTYHALYPRAWTIY+GEPDPELR
Sbjct: 61  PNGEKYSTVLCAQSPENQREVESSGIGSWDWNLKGHSSTYHALYPRAWTIYNGEPDPELR 120

Query: 204 IVCRQISPVIPHNYKESSYPVSVFTFTLHNSGKTDADVSLLFTWANSVGGLSEYSGNHIN 263
           IVCRQISPVIPHNYKESSYPVSVFTFTLHNSGKTDADV+LLFTWANSVGGLSEYSGNHIN
Sbjct: 121 IVCRQISPVIPHNYKESSYPVSVFTFTLHNSGKTDADVNLLFTWANSVGGLSEYSGNHIN 180

Query: 264 SRTKKKDGVHTVLLHHKTASGFPPVTYAIAAQEGNGVHVSDCPCFVISGNSQGISAKDMW 323
           SRTKKKDGVHTVLLHHKTASG+PPVTYAIAAQEGNGVHVSDCPCFVISG+SQGISAKDMW
Sbjct: 181 SRTKKKDGVHTVLLHHKTASGYPPVTYAIAAQEGNGVHVSDCPCFVISGHSQGISAKDMW 240

Query: 324 LEIKEHGSFDRLKFADMSMPSEVGSSIGAAVSASVTVSSDSVRTVTFSLSWDCPEVNFCR 383
           LEIKEHGSFDRLKFADMSMPSEVGSSIGAA++AS+TVSSDSVRTVTFSLSWDCPEVNFCR
Sbjct: 241 LEIKEHGSFDRLKFADMSMPSEVGSSIGAAITASITVSSDSVRTVTFSLSWDCPEVNFCR 300

Query: 384 GKTYHRRYTKFYGNLGDAAADIARDAILEHHHWESQIDAWQRPVLEDKRFPKWYPVTLFN 443
           GKTYHRRYTKFYGNLGDAAADIARDAILEHHHWESQIDAWQRPVLEDKRFPKWYPVTLFN
Sbjct: 301 GKTYHRRYTKFYGNLGDAAADIARDAILEHHHWESQIDAWQRPVLEDKRFPKWYPVTLFN 360

Query: 444 ELYYLNAGGTIWTDGSLPIQSLVSIGERGFCLDQCRSG-DSVIYESHENDTANDILGRMT 503
           ELYYLNAGGTIWTDGSLPIQSLVSIGERGFCLDQCRSG  SVIYESHENDTANDILGRMT
Sbjct: 361 ELYYLNAGGTIWTDGSLPIQSLVSIGERGFCLDQCRSGLKSVIYESHENDTANDILGRMT 420

Query: 504 SRLDELRDSVASNSAFGVNLLQKGEENVGQFLYLEGVEYTMWNTYDVHFYSSFAIIMLFP 563
           SRLDELRDSV SNSAFGVNLLQKGEENVGQFLYLEGVEYTMWNTYDVHFYSSFAIIMLFP
Sbjct: 421 SRLDELRDSVESNSAFGVNLLQKGEENVGQFLYLEGVEYTMWNTYDVHFYSSFAIIMLFP 480

Query: 564 KLELSIQRDFAAAVMMHDPSKMQLLDNGKWEARNVLGAVPHDIGVNDPWFEVNGYNLYNT 623
           KLELSIQRDFAAAVMMHDPSKMQLLDNGKWEARNVLGAVPHDIGVNDPWFEVNGYNLYNT
Sbjct: 481 KLELSIQRDFAAAVMMHDPSKMQLLDNGKWEARNVLGAVPHDIGVNDPWFEVNGYNLYNT 540

Query: 624 DRWKDLNPKFVLQIYRDVVATGDMKFAKAVWPPVYLAIAYMDQFDRDGDGMIENDGFPDQ 683
           DRWKDLNPKFVLQIYRDVVATGDMKFAKAVWPPVYL+IAYMDQFDRDGDGMIENDGFPDQ
Sbjct: 541 DRWKDLNPKFVLQIYRDVVATGDMKFAKAVWPPVYLSIAYMDQFDRDGDGMIENDGFPDQ 600

Query: 684 TYDTWSVSGVSAYSGGLWVAALQAASALARVADEKDAEHYFWFKFQKAKRAYEKLWNGSY 743
           TYDTWSV+GVSAYSGGLWVAALQAASALARV DEKDAEHYFWFKFQKAKRAYEKLWNGSY
Sbjct: 601 TYDTWSVTGVSAYSGGLWVAALQAASALARVVDEKDAEHYFWFKFQKAKRAYEKLWNGSY 660

Query: 744 FNYDSSGGSSSSSIQADQLAGQWYARASGLLPIVDEDKAKSALAKVYNYNVMKVKDGKRG 803
           FNYDSSGGSSSSSIQADQLAGQWYARASGLLPIVDEDKAKSALAKVYNYNVMKVKDGKRG
Sbjct: 661 FNYDSSGGSSSSSIQADQLAGQWYARASGLLPIVDEDKAKSALAKVYNYNVMKVKDGKRG 720

Query: 804 AVNGMLPDGTIDFSSMQSREIWSGVTYAVAASMIHEDMTDMAFRTAEGIHEAAWSEDGLG 863
           AVNGMLPDGT+DFSSMQSREIWSGVTYAVAASMIHE+MTDMAFRTA+GIHEAAWSEDGLG
Sbjct: 721 AVNGMLPDGTVDFSSMQSREIWSGVTYAVAASMIHENMTDMAFRTAQGIHEAAWSEDGLG 780

Query: 864 YNFQTPEAWTTTDRYRSLCYMRPLAIWAMQWAFSEKVSVIEELKELDSDAISRHHARFSK 923
           YNFQTPEAWTTTDRYRSLCYMRPLAIWAMQWAFSEKVSVIEELKELDSDAI RHHA+FSK
Sbjct: 781 YNFQTPEAWTTTDRYRSLCYMRPLAIWAMQWAFSEKVSVIEELKELDSDAILRHHAKFSK 840

Query: 924 VARYLKLPEDGTSSSVLQTVYDYTLKRFF 952
           VAR LKLPEDGTSSSVLQTVYDYTLKRFF
Sbjct: 841 VARLLKLPEDGTSSSVLQTVYDYTLKRFF 869

BLAST of Cucsa.023010 vs. NCBI nr
Match: gi|700194399|gb|KGN49576.1| (hypothetical protein Csa_5G011660 [Cucumis sativus])

HSP 1 Score: 1580.5 bits (4091), Expect = 0.0e+00
Identity = 766/776 (98.71%), Postives = 766/776 (98.71%), Query Frame = 1

Query: 1   MSEGKKLGNGLVEQDEDINKSLTEK----------TGIDPGQTPSLTWQRKLNREGTSLW 60
           MSEGKKLGNGLVEQDEDINKSLTEK          TGIDPGQTPSLTWQRKLNREGTSLW
Sbjct: 55  MSEGKKLGNGLVEQDEDINKSLTEKVKWCIQSFFMTGIDPGQTPSLTWQRKLNREGTSLW 114

Query: 61  QFRLHLKEIIHLAPVGYRLFRYIREESVKGRGAMINPFIRRRITDTHAIPLGGLGSGSIG 120
           QFRLHLKEIIHLAPVGYRLFRYIREESVKGRGAMINPFIRRRITDTHAIPLGGLGSGSIG
Sbjct: 115 QFRLHLKEIIHLAPVGYRLFRYIREESVKGRGAMINPFIRRRITDTHAIPLGGLGSGSIG 174

Query: 121 RSYRGEFQRWQLFPRKCEDKPILANQFSVFVSRPNAEKYSTVLCAQSPETQREVESSGIG 180
           RSYRGEFQRWQLFPRKCEDKPILANQFSVFVSRPNAEKYSTVLCAQSPETQREVESSGIG
Sbjct: 175 RSYRGEFQRWQLFPRKCEDKPILANQFSVFVSRPNAEKYSTVLCAQSPETQREVESSGIG 234

Query: 181 SWDWNLKGHSSTYHALYPRAWTIYDGEPDPELRIVCRQISPVIPHNYKESSYPVSVFTFT 240
           SWDWNLKGHSSTYHALYPRAWTIYDGEPDPELRIVCRQISPVIPHNYKESSYPVSVFTFT
Sbjct: 235 SWDWNLKGHSSTYHALYPRAWTIYDGEPDPELRIVCRQISPVIPHNYKESSYPVSVFTFT 294

Query: 241 LHNSGKTDADVSLLFTWANSVGGLSEYSGNHINSRTKKKDGVHTVLLHHKTASGFPPVTY 300
           LHNSGKTDADVSLLFTWANSVGGLSEYSGNHINSRTKKKDGVHTVLLHHKTASGFPPVTY
Sbjct: 295 LHNSGKTDADVSLLFTWANSVGGLSEYSGNHINSRTKKKDGVHTVLLHHKTASGFPPVTY 354

Query: 301 AIAAQEGNGVHVSDCPCFVISGNSQGISAKDMWLEIKEHGSFDRLKFADMSMPSEVGSSI 360
           AIAAQEGNGVHVSDCPCFVISGNSQGISAKDMWLEIKEHGSFDRLKFADMSMPSEVGSSI
Sbjct: 355 AIAAQEGNGVHVSDCPCFVISGNSQGISAKDMWLEIKEHGSFDRLKFADMSMPSEVGSSI 414

Query: 361 GAAVSASVTVSSDSVRTVTFSLSWDCPEVNFCRGKTYHRRYTKFYGNLGDAAADIARDAI 420
           GAAVSASVTVSSDSVRTVTFSLSWDCPEVNFCRGKTYHRRYTKFYGNLGDAAADIARDAI
Sbjct: 415 GAAVSASVTVSSDSVRTVTFSLSWDCPEVNFCRGKTYHRRYTKFYGNLGDAAADIARDAI 474

Query: 421 LEHHHWESQIDAWQRPVLEDKRFPKWYPVTLFNELYYLNAGGTIWTDGSLPIQSLVSIGE 480
           LEHHHWESQIDAWQRPVLEDKRFPKWYPVTLFNELYYLNAGGTIWTDGSLPIQSLVSIGE
Sbjct: 475 LEHHHWESQIDAWQRPVLEDKRFPKWYPVTLFNELYYLNAGGTIWTDGSLPIQSLVSIGE 534

Query: 481 RGFCLDQCRSGDSVIYESHENDTANDILGRMTSRLDELRDSVASNSAFGVNLLQKGEENV 540
           RGFCLDQCRSGDSVIYESHENDTANDILGRMTSRLDELRDSVASNSAFGVNLLQKGEENV
Sbjct: 535 RGFCLDQCRSGDSVIYESHENDTANDILGRMTSRLDELRDSVASNSAFGVNLLQKGEENV 594

Query: 541 GQFLYLEGVEYTMWNTYDVHFYSSFAIIMLFPKLELSIQRDFAAAVMMHDPSKMQLLDNG 600
           GQFLYLEGVEYTMWNTYDVHFYSSFAIIMLFPKLELSIQRDFAAAVMMHDPSKMQLLDNG
Sbjct: 595 GQFLYLEGVEYTMWNTYDVHFYSSFAIIMLFPKLELSIQRDFAAAVMMHDPSKMQLLDNG 654

Query: 601 KWEARNVLGAVPHDIGVNDPWFEVNGYNLYNTDRWKDLNPKFVLQIYRDVVATGDMKFAK 660
           KWEARNVLGAVPHDIGVNDPWFEVNGYNLYNTDRWKDLNPKFVLQIYRDVVATGDMKFAK
Sbjct: 655 KWEARNVLGAVPHDIGVNDPWFEVNGYNLYNTDRWKDLNPKFVLQIYRDVVATGDMKFAK 714

Query: 661 AVWPPVYLAIAYMDQFDRDGDGMIENDGFPDQTYDTWSVSGVSAYSGGLWVAALQAASAL 720
           AVWPPVYLAIAYMDQFDRDGDGMIENDGFPDQTYDTWSVSGVSAYSGGLWVAALQAASAL
Sbjct: 715 AVWPPVYLAIAYMDQFDRDGDGMIENDGFPDQTYDTWSVSGVSAYSGGLWVAALQAASAL 774

Query: 721 ARVADEKDAEHYFWFKFQKAKRAYEKLWNGSYFNYDSSGGSSSSSIQADQLAGQWY 767
           ARVADEKDAEHYFWFKFQKAKRAYEKLWNGSYFNYDSSGGSSSSSIQADQLAGQWY
Sbjct: 775 ARVADEKDAEHYFWFKFQKAKRAYEKLWNGSYFNYDSSGGSSSSSIQADQLAGQWY 830

BLAST of Cucsa.023010 vs. NCBI nr
Match: gi|1000947255|ref|XP_015580669.1| (PREDICTED: non-lysosomal glucosylceramidase isoform X1 [Ricinus communis])

HSP 1 Score: 1462.6 bits (3785), Expect = 0.0e+00
Identity = 709/951 (74.55%), Postives = 801/951 (84.23%), Query Frame = 1

Query: 1   MSEGKKLGNGLVEQDEDINKSLTEKTGIDPGQTPSLTWQRKLNREGTSLWQFRLHLKEII 60
           MSEG+   NG  ++D + + SL EK  +DPG   SLTWQRKLN E  +L QF L  +E  
Sbjct: 3   MSEGEIPANGC-QEDREPSDSLLEK--VDPGSPASLTWQRKLNSEDIALSQFNLSFQEKF 62

Query: 61  HLAPVGYRLFRYIREESVKGRGAMINPFIRRRITDTHAIPLGGLGSGSIGRSYRGEFQRW 120
            LAPVG RL+R IREE+ KGR  +INPF++R IT  H IPLGG+GSGSIGRSY+GEFQRW
Sbjct: 63  QLAPVGIRLWRLIREETAKGRAGIINPFLKRFITSCHGIPLGGIGSGSIGRSYKGEFQRW 122

Query: 121 QLFPRKCEDKPILANQFSVFVSRPNAEKYSTVLCAQSPETQREVESSGIGSWDWNLKGHS 180
           QLFPR CE+KP+LANQFSVFVSR + EKYS+VLC ++PE   E   SGIGSWDWNLKG +
Sbjct: 123 QLFPRICEEKPVLANQFSVFVSRSSGEKYSSVLCPRNPEVLMEPAISGIGSWDWNLKGDN 182

Query: 181 STYHALYPRAWTIYDGEPDPELRIVCRQISPVIPHNYKESSYPVSVFTFTLHNSGKTDAD 240
           STYHALYPRAWTIYDGEPDPELRIVCRQISP+IPHNYKESSYPVSVFTFTL+NSGKT AD
Sbjct: 183 STYHALYPRAWTIYDGEPDPELRIVCRQISPIIPHNYKESSYPVSVFTFTLYNSGKTTAD 242

Query: 241 VSLLFTWANSVGGLSEYSGNHINSRTKKKDGVHTVLLHHKTASGFPPVTYAIAAQEGNGV 300
           VSLLFTW NSVGG SEYSG H NS T  +DGVH VLLHHKTA GFPPVT+AIAAQE N V
Sbjct: 243 VSLLFTWTNSVGGNSEYSGQHFNSTTMMEDGVHAVLLHHKTAEGFPPVTFAIAAQETNDV 302

Query: 301 HVSDCPCFVISGNSQGISAKDMWLEIKEHGSFDRLKFADMSMPSEVGSSIGAAVSASVTV 360
           HVS CP FVISGN QGI+AKDMW E+KEHGSFD LK A  S PSE GSSIGAA++ASVT+
Sbjct: 303 HVSKCPRFVISGNCQGITAKDMWHEVKEHGSFDNLKSAGTSGPSEPGSSIGAAIAASVTI 362

Query: 361 SSDSVRTVTFSLSWDCPEVNFCRGKTYHRRYTKFYGNLGDAAADIARDAILEHHHWESQI 420
             D++R+VTFSLSWDCPEV F  G+TYHRRYTKFY   GDAAA IA DAILEH  WESQI
Sbjct: 363 PPDAIRSVTFSLSWDCPEVYFMGGRTYHRRYTKFYSTHGDAAARIAHDAILEHGLWESQI 422

Query: 421 DAWQRPVLEDKRFPKWYPVTLFNELYYLNAGGTIWTDGSLPIQSLVSIGERGFCLDQCRS 480
            AWQRP+LEDKR P+WYP+TLFNELYYLN+GGTIWTDGS P  +LVSI    F LD   +
Sbjct: 423 VAWQRPILEDKRLPEWYPITLFNELYYLNSGGTIWTDGSPPYHNLVSIRGSKFSLDTSGA 482

Query: 481 G-DSVIYESHENDTANDILGRMTSRLDELRDSVASNSAFGVNLLQKGEENVGQFLYLEGV 540
           G  S+I  +HENDTA +ILGRMTS L+++   VASNSAFG NLLQ+GEEN+GQFLYLEG+
Sbjct: 483 GLKSIIDVTHENDTAVNILGRMTSALEQIHAHVASNSAFGTNLLQEGEENIGQFLYLEGI 542

Query: 541 EYTMWNTYDVHFYSSFAIIMLFPKLELSIQRDFAAAVMMHDPSKMQLLDNGKWEARNVLG 600
           EY MWNTYDVHFYSSFA++MLFPKLELS+QRDFAAAVMMHDPSKMQLL +G+W  R VLG
Sbjct: 543 EYHMWNTYDVHFYSSFALVMLFPKLELSVQRDFAAAVMMHDPSKMQLLHDGQWVCRKVLG 602

Query: 601 AVPHDIGVNDPWFEVNGYNLYNTDRWKDLNPKFVLQIYRDVVATGDMKFAKAVWPPVYLA 660
           AVPHDIG+NDPW+EVN Y+LYNTDRWKDLNPKFVLQ+YRDVVATGD KFA+AVWP VY+A
Sbjct: 603 AVPHDIGINDPWYEVNAYSLYNTDRWKDLNPKFVLQVYRDVVATGDKKFAEAVWPSVYIA 662

Query: 661 IAYMDQFDRDGDGMIENDGFPDQTYDTWSVSGVSAYSGGLWVAALQAASALARVADEKDA 720
           +AYMDQFDRDGDGMIENDGFPDQTYDTWSVSGVSAYSGGLWVAALQAASALAR   +K +
Sbjct: 663 MAYMDQFDRDGDGMIENDGFPDQTYDTWSVSGVSAYSGGLWVAALQAASALAREVGDKGS 722

Query: 721 EHYFWFKFQKAKRAYEKLWNGSYFNYDSSGGSSSSSIQADQLAGQWYARASGLLPIVDED 780
           E YFW +FQKAK  Y+KLWNGSYFNYD+SGG +SSSIQADQLAGQWYARA GL PIVD+D
Sbjct: 723 EDYFWARFQKAKDVYDKLWNGSYFNYDNSGGRNSSSIQADQLAGQWYARACGLFPIVDKD 782

Query: 781 KAKSALAKVYNYNVMKVKDGKRGAVNGMLPDGTIDFSSMQSREIWSGVTYAVAASMIHED 840
           KA+SAL KVYNYNV+KVKDGKRGA+NGMLPDG +D SSMQSREIWSGVTYA+AA+MI ED
Sbjct: 783 KARSALEKVYNYNVLKVKDGKRGAINGMLPDGKVDLSSMQSREIWSGVTYALAATMIQED 842

Query: 841 MTDMAFRTAEGIHEAAWSEDGLGYNFQTPEAWTTTDRYRSLCYMRPLAIWAMQWAFSEKV 900
           M DMAF TA GI+EAAWSE GLGY+FQTPEAW   D+YRSLCYMRPLAIWAMQWA S   
Sbjct: 843 MLDMAFHTASGIYEAAWSERGLGYSFQTPEAWNNVDQYRSLCYMRPLAIWAMQWALSRPK 902

Query: 901 SVIEELK-ELDSDAISRHHARFSKVARYLKLPEDGTSSSVLQTVYDYTLKR 950
              EE++ E++ D +  HHA F+KVAR+L+LPE   S  +LQ++++YT K+
Sbjct: 903 LEKEEMEMEVNEDYLLPHHAGFTKVARFLRLPEGEESLGLLQSLFNYTCKK 950