Csa5G011660 (gene) Cucumber (Chinese Long) v2

NameCsa5G011660
Typegene
OrganismCucumis. sativus (Cucumber (Chinese Long) v2)
DescriptionNon-lysosomal glucosylceramidase; contains IPR014551 (Beta-glucosidase, GBA2 type), IPR024462 (Beta-glucosidase, GBA2 type, N-terminal)
LocationChr5 : 376321 .. 384898 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CAAGAATTGGTAAACGAAATTTGCATGAATTGATGACGAGACAGAGAGAGAAAAATCGGATTGAGTTGCAATTCACTAATTCTATATGAATAAGCAATGGAGAATTTTCCATTTCCGCCACTGGAGCTCTCATTAAGGCTTTCTCTTCTTCTTCTTCTTCTTCAATTCCACTTCCCTTTTTCCTCATCATCCTTATCATGTATGTCTTCTTTGATTTCCCTTTTTCTCTCTACTTCTCTCGTTTTTTCCGGTTCAGTGTAATTCGATTCAACTTGATTGGCTTTTTGTCTTCCTTAATTCCCCGGCTTTATACTATAATTTTAGTTTTATTTTCCCAATTGCTTGATTGAACTTGTTTTTTTTTGTCCTGTACAATGCAATCTGAGGTTGACTTTTTTCTTTTTTTCATCTTGATCTTTTGACTCAACTGATTTGGGTTTTGATTTTCCTCGATTCCCATGCTTTTTTGCTTCAACTTCTGAATTTTTATTATTCGAAATTTTATAGAATTCTTGTTTGGGATGAGAAGTATGATTTCTTGAACACTTGGATATTTTTCTCATCGTATAGTTGGCTTTAATTTGTATTGAGAAAAATAAGAATTTAGGCCTGGATTGATAACAATTTAGTTTTTTAGTTTTCTTGTTTTGAAATTTATGTTGGTTTCCTCCCAATTCACAGATCTTGTGAATCAGTGTTTTAGAACTCAACAAGTTTGAGTGGGATGAGATCAAGTTTTTCGTGTTGTATTTATTCAGCAGAGTATGCCCATATTTATACACTCGTAACCCATACAAGGAAGAAACTAACTCCCACCATAATAGGAACACATTCGTAACAATACAAAAATCACCAATAGATGAAGAATAAAATAAATATCCTGCTAACAACCTGAACAGCCATAGGTGGTGGACTTGGCACGTTTGTGATGAGCTGGCGCAGGTGGATCGGGTAGGCTCCCTAATATTGTGGAAACAGTAGAGTTCCTAGTCAAATTCTAATAACAAAAACTATTTTTCTTTTCAAAACTTGGCATGCTTTTTGAAAACAAGAGTAGAACATCGACAACAAACCATAGAAACCTATAGATGGAAGTAGTGTTCATAGCTTAATTTTCCAAAATTAGATAATGATCAGATGCGGCTTTAATTTCCTACTTTGTGGTTATTAACTTCACCATCTCTATCTCTGTGTGTGCGAACGTTTTGTGTATGTATGCCAATTGTAATTGTGATGCAGAAAAAATTAAGAATGATGTTTGGATTAGGGGTCATTTCAGTTCTTGTTGGTCTCCCATCTCTTTGATTATCGTAGGCTTTATGGATATAACTTCACTTATAACTGTATCATTTTTTCCCCATGTTGACAGAAATATGTCAGAAGGCAAGAAGTTGGGAAATGGCTTGGTTGAGCAAGATGAGGACATCAATAAATCTCTAACTGAAAAGGTTAAATGGTGCATTCAATCATTTTTTATGGTTAAAGATGATCTGATCCATTTTCAATGACATTCAAAATAGCTTTACCTTTTGCATAATATGCTATAAATGGGGATAAGCTCTCTGTATTATCTGTGCATCTGATCTACTCTTACGGATGCTTTTTGAATTTGAGTAATTATTAAAGAATGGTATGTCAAATATTTTGTGATTAAAATGTAAATTTTAAAGTTTGAGTCCTTTTTTAGCCCAATATTGCAGAGTAAGGATGTTAAGAAGCCAAAGAAGTTACCCTTTAGGAGAGGATACTTTTTAAAGAGGGAATTATTTTCTAGAAGATATATATATTTTTTGAATCAGACAGTATTTGCTTTCTCATTTCCTCACAGAAGAAGCTTTGGGAGTATTCGACCCACCGATTTTATAAGTTGGTGTTAAATAACACAACTACACTAATTTCAATAGTGTTCACTATTGAAGTTTTCACTTTTATCTAGGAACTCCATATTTTCCTCTTTCTCCCTTTTGCACATTTATTGAGAAACTCCATTTTTACAATTTTACACTCAAAACATAGACTTCCTAACTCCAACATATCAAGTCTAAACTTCATAACTAAACTCAGTGCCCCAAACACTTGCTCAACATTCTAAATAATCCTCCTATGTTAAGGCTTCTCACATATTTCAAGGGGGAAATGGTTCCGTTTTGTCTATGACAGAATAAACAGAACTAAACTGTTCAATTAATGGCCACTAATTTAAAAAAGCATGCCTAGAAGTATCCGTAGACTTCGCTTGAATTTACATCTTTGTGTTCCTTGTTGTGATCGTGGTCCTTGGAATATTTGAGAGGCTAATTCCTTCCTTTCAAGCCTTGGAAACGGTTGCAGTGCTAAAAACAAGGGAGACTAATCTTTATTTATAGTAAGGGATATATGCATCTAGTAATATTAATTCCTAGAGAATGCCTATTCCTATTGATTATTATATATATCATAGCCTGAACTTTCCTCAATTTGTATAGACTGGCATTGATCCAGGGCAAACTCCATCACTCACCTGGCAAAGAAAATTAAACAGGGAAGGAACTTCACTTTGGCAGTTCAGGTTGCATCTGAAAGAGATAATTCATCTGGTATTTTTAAATTTAAGATGATTTCTTTTAAAATCTGTATTGTACCGTCATAATCTTGTGTGTTGATAATATTAACATGTATGATAGCTGTTCAGTATTACTTGGTGTCCAAGCAGTTAAAAAAACTTGGTATTCATTATTTTATTTCTTTAATATCAGAATTTGATAGACACTCCATCTAGGATTGGTCTTATTTTATAGATATGATAGTGTGAAAATACATGCTTGTCTATTTTATAAAATAAATGTTTTTACACTTTTCCGGTACTTAGAGCATCCAAAATATGCAGTTTAAGGTCATGGTTTGAACTATCAGGTATGTCATATTGCTCACTCTTCTTCTCTTCCAGGCTCCAGTAGGGTATAGGCTATTCCGCTATATACGAGAAGAATCTGTCAAAGGAAGGGTAGTAATTTTATTGTAATATTGCTAGTTCAGATTGATTCTTAGGTTTGTAAGTAAATGGACTTAGAAATTATTGAATAGCTCTTCTTGTCTTCATTTCAGGGGGCTATGATAAATCCTTTTATCAGACGTCGTATAACTGATACTCATGCCATTCCTCTAGGTGGTCTTGGGTAAGTATTTCTATCTTTATATTTTTTCCTTTTCGAATTTAGTACAACATGCACCTTACGATATCCTTACTTTATATCAATTTTCATTGTATGGATATCACTCCAAAAATCATTGAATATCAATTTTCATTGCATTTATGATTTACATGTGGAAAATAAAACATTGACTTGATGTTGGACATGGAAAAAGTGATTGTAAGCATTTAAAGAACACTCCCAAACATGCTCTTAGTCTATTTTCATTGTTCAAGAGTGATATGATGATTATTTGAAGTGGTTAATTATGTAAGCTTGCGATACATTCAGATTGATCTGAGAATTATATGTAACTATTCTTTTAATTTTTTGTTTTGCTATCTAACAATCTAAATGTTTAGATCAGGAAGCATTGGAAGAAGTTACCGAGGTGAATTTCAACGATGGCAACTATTCCCTAGAAAATGTGAAGATAAGCCAATTTTAGCAAATCAATTTTCTGTGAGTTCATATGTCCTTCCTTGCTATCTTGAAACGCTTTTTAATGTGTATCATACACATTGTAATTTCATTTACATTCTTGGGACTCATTAGTTATAGAAAAGTTTGAGTCAGTTCAATTATTAATAATAATCTAGTTACAAGTACTTCAATAGGTTTTCGTCTCACGACCGAACGCTGAGAAGTATTCTACTGTACTATGTGCACAGAGCCCTGAAACTCAGAGGTAGATACTAAACTTTTTATTGACATCTAATAAATCTACATTTGACCATTGTATTGTTTAAAAATTGTAAGATCGTGAGGTTTTCCAACCTAATGTGGAATCCTCAACATGCTCCTTCAAAGATAGTGCTTCTTAGAGTTCACCATTCTTGAGTCGTTGGACCCAATTTTGGACTGAATGGCTATTTTGGCTTCATGGACTGATATCATAAAGATTGTGAGGTATCATGTTTTCAACGTGAGATCCTCATAATTGTTGAATGGGATAACTAAAAAATAATTGTCTACCTGCCTTCTCATTATCATATGCACCGTCAGGGAAGTTGAATCATCAGGCATTGGATCTTGGGATTGGAATTTGAAGGGTCATAGCTCTACATATCATGCTCTGTATCCTCGAGCTTGGACGATATATGATGGTATTTTTTTAATCTTACATTTTATGATTTGTTGGTTCATCATTGTTTATATAACGTCCAGTTCATTTTTCACCCTTTTCCTCCAATGCAGGTGAACCGGATCCAGAACTTAGAATAGTTTGCCGTCAGATTTCACCTGTTATTCCTCATAATTACAAGGAGAGCAGTTACCCTGTCTCAGTTTTTACTTTCACGGTAGAAATGAATTTATGTATTTACATTTATTTTATCCTTTTTTTTATGCTGTTGTTGGTCTTATGAATTTTAGTTCACCATTGGCCTCTTGATATATTTTCCATTTCAGCTGCATAATTCTGGAAAAACAGATGCTGACGTCAGTTTGCTTTTCACCTGGGCAGTATGTGTTCATCTCGATTATTAATTTGATTATAATTTCCTTTCCTAATGTTATGCCATGAACTAATTATTCTTCTTTTAGATTATCACTTTGAATTTGTCTGGATTTATCTAGAATCATGAGATTCTTTGGACTTGATTTTTCTTTTCTCTTGTTATTCAGAATTCAGTTGGAGGGCTTTCTGAGTATTCTGGTAACCATATCAATTCGAGAACAAAGTAAGAAAGTAATACTAGCTAGACCTCATTATTATTCTTTTCTTTATTATCTTAATTACGACTACAAGTGTTGCTTTAATTAAAATCTTTCTCATGCATTTGTAGAGAATTATCATTAATCATTAGTTCAAATCTAACCTACAATATTTATGATTTCTTTAGGAAGAAAGATGGCGTGCATACTGTGCTTCTACATCACAAGCAAGTGCCTTTCTTGGTCATTTATTAAGGGGATTGAGACAGGGTGTTATTAGAGCAATAATCAATCATATATTATATGTTTTATATTTCTTATTGTCTTCTCTTTTAAATAATCAGGACAGCAAGTGGATTTCCCCCTGTGACGTATGCTATTGCCGCTCAAGAGGGCAATGGGGTTCATGTCTCAGATTGCCCTTGTTTTGTAATATCTGGTAATTCCCAGGGTATATCAGCCAAGGATATGTGGCTTGAGATCAAAGAGGTATTTAGCTTGATGTATTTTACTGTCCATGCTACAATTATTATTAAAATTTGGGCACATATCCATTTACACCTTAAACTTTGGGAGCCGTATCAACTAAAATCATGAAATAATAATTGCATCAGCTTAAATGTCGAAGAACTTTCATAGTCCTTTGATTTATGTTCGATCAATATCGTGTGAAACATATGATTTTGAGTCTCAATTAAACTCATAAATGTTCTAAGTCATCAATTTAGATCTTTCATTAGGGTTTACCTTTGAAAATCTATTTTTATTAAAATTTGGGCAAATATCCATTTATACCTTAAACTTTGGGGGCTGTATCAATTAAAAGCATGAACTGATAATTGCATCAATTTAAATCTCGAAGCACTTCTGTAGTTCCTTAAATTTTGTTCGATCAATATCGTGTGAAATATATGATTTTGAGTCAATTAAACTCATCAATGTTCTAAGTCATCAATTTATATCTTTCTTTAGGGTTACCTTTGAAAATCTTTCATACATTGATTCTCAAACGTGTGAGGTCTTTTTCAAATGTTGTCTTTACAAAATGGATGATTAGAGTAGATGCATGGGATGATTTTCAAAGGGAATTCTGACTAAGAGTCTGAATGATCTATTTACGAATGTGTAGGTGTTTAATGGTTACACACAAGTTTTGAGGTAAATTGACACACTTACAAAAGTTAGGGGTTTGAATTGATATAATTACTGGTTTAAGGTTTAAATCGATACAACCCTAAAGTTTTATGACTATAAATTGATATTTTCCCTTAAACAATCATTTCTATTATTACTTGCTTCCTTTTGGAGCTTTACATGTCTATTATTGTTGTAATAGCATGGATCGTTTGATCGGCTTAAATTTGCTGATATGTCAATGCCCTCTGAAGTGGGTTCGTCCATTGGTGCGGCCGTTTCAGCTTCAGTTACAGTTTCATCTGATTCTGTTCGTACTGTGACATTTTCGTTGTCATGGGACTGTCCTGAAGTGAACTTTTGTAGAGGAAAAACTTATCACAGGTTAGTGGCAAAATTGTACAACAAATGATACAACCTGAATCAATCATATTATCTATGTGATCAAAGATGATTTTCTGTGCAGGCGTTACACAAAATTCTATGGTAACCTTGGTGATGCTGCTGCCGATATTGCACGTGATGCTATACTAGGTAATTTTTAGTTGTATCGTGTAATTAGGTTGTCTAAACCACAATTTGTGTGAACATTTGGACAGTCTAAAATTTGTTGGACACGTATAAGACATTTATTAGAACAACAAATGTATTAGACATGCATCAAATATTTGTTGAGTAGACTAAAGAGATATATATGACAGTAATAATAAATTGTGTAAATTACATCAAACTAATTTTCTAAAATATATAATGCACCAACCTATTTACTTTTAATATGATGTGAAAGTGATACATGTTTTTGAAAATGTCTACTTTAATAAACATGTCATTGTGGTGTGCGTGCCCTCAAATTTTTTAAAAATGACATGTCACTGTGTTCTTATCGTGTTGTATTCATTCTCCTATTTGTATATGTGTTCTTAGATTCATTGTTTCTTAATTGCTTACTGCAATCCTCAAATAGAATAGAAATAATCAAATTATATAGTTTATTTGTTTATGTAGTTAACTTCTGGAACTACAAATTTGTCTTTCTTTCCCTTTAGAAGAAATGTTGCTCTGATGTTCACTCCTGTTAGAAAATTTCTCCATCATATCACTCACCATTAAATTGAATCCTCCTTGTGCACCTACTTTTTCGAAAGACTGATGAGCTTGAATCTATTATTAAAAGGAGGCATTGCGTAACTTTCTTCATGTAATTATTTCCAAAAATACGTTAGCTTAACATCCTGTGTTTCTGTGACCGACTTAGAACATCATCACTGGGAATCTCAAATTGATGCTTGGCAAAGACCTGTTCTTGAAGACAAGAGGTTTCCAAAATGGTAGGCTTTGTTATATCGGTTATTGTTCATCGATTCCTTCAGATTTTGAAGTTTCTGGGTTTATGCAGGTATCCTGTTACTCTCTTTAATGAGCTTTATTATCTAAATGCAGGAGGGACAATCTGGACAGGTATATCTAACCTACCATTTTAAGATTGCTATTTATGATTTTGATTAAAATTGTATCATTTTTTAAAATGTTTGTATAGATGGGTCGCTTCCAATTCAGAGTTTAGTAAGCATAGGGGAAAGAGGATTTTGCCTTGATCAATGCAGATCTGGTGATAGTGTAATTTATGAGTCCCATGAAAATGACACTGCTAATGATATTCTTGGGAGGATGACTTCAAGACTTGATGAGTTACGTGATTCAGTCGCGTCAAATTCTGCATTTGGAGTAAATCTGCTTCAAAAGGGGGAGGAGAATGTTGGGCAGTTCCTTTACCTTGAAGGGGTTGAATACACAATGTGGAATACCTATGACGTTCATTTTTACTCCTCTTTTGCAATAATAATGCTATTTCCAAAACTCGAACTCAGCATTCAACGAGATTTTGCAGCAGCAGTGATGATGCATGATCCCAGCAAGATGCAACTTCTTGATAATGGAAAATGGGAAGCAAGAAATGTTCTTGGAGCTGTTCCTCATGACATCGGAGTTAATGATCCATGGTTTGAAGTGAATGGATATAATCTATATAATACAGATAGGTGGAAGGACTTGAATCCAAAATTTGTCCTCCAAATTTATAGGGATGTTGTTGCCACAGGGGATATGAAATTTGCAAAGGCTGTCTGGCCCCCTGTCTACCTTGCCATAGCTTATATGGATCAGTTCGATCGAGATGGTGATGGGATGATTGAGAATGATGGGTTCCCCGATCAGACTTATGATACGTGGTCTGTCTCTGGTGTTAGTGCATATAGTGGTGGCTTATGGGTGGCAGCGTTGCAGGCTGCTTCAGCCCTGGCTCGTGTAGCCGATGAAAAGGATGCTGAGCACTATTTTTGGTTCAAATTTCAGAAGGCAAAACGTGCCTATGAGAAATTGTGGAATGGTTCTTACTTCAACTATGACAGTAGTGGCGGGAGTTCAAGTTCATCCATTCAAGCTGATCAGTTGGCTGGACAATGGTATATATGAAATAAAATCTTGAAGTTTTTTAATAATAACAAAAAGTTTAGCATAATGCCATACAACTCAAAGGAGTTATCACTTTAATATACTTCTTTTTCTAATGCATTAGCAGTTAGGTTTTACCTTAGTCTGGACTAGAATCTAGAATTAAA

mRNA sequence

ATGGAGAATTTTCCATTTCCGCCACTGGAGCTCTCATTAAGGCTTTCTCTTCTTCTTCTTCTTCTTCAATTCCACTTCCCTTTTTCCTCATCATCCTTATCATGCTTTATGGATATAACTTCACTTATAACTGTATCATTTTTTCCCCATGTTGACAGAAATATGTCAGAAGGCAAGAAGTTGGGAAATGGCTTGGTTGAGCAAGATGAGGACATCAATAAATCTCTAACTGAAAAGGTTAAATGGTGCATTCAATCATTTTTTATGACTGGCATTGATCCAGGGCAAACTCCATCACTCACCTGGCAAAGAAAATTAAACAGGGAAGGAACTTCACTTTGGCAGTTCAGGTTGCATCTGAAAGAGATAATTCATCTGGCTCCAGTAGGGTATAGGCTATTCCGCTATATACGAGAAGAATCTGTCAAAGGAAGGGGGGCTATGATAAATCCTTTTATCAGACGTCGTATAACTGATACTCATGCCATTCCTCTAGGTGGTCTTGGATCAGGAAGCATTGGAAGAAGTTACCGAGGTGAATTTCAACGATGGCAACTATTCCCTAGAAAATGTGAAGATAAGCCAATTTTAGCAAATCAATTTTCTGTTTTCGTCTCACGACCGAACGCTGAGAAGTATTCTACTGTACTATGTGCACAGAGCCCTGAAACTCAGAGGGAAGTTGAATCATCAGGCATTGGATCTTGGGATTGGAATTTGAAGGGTCATAGCTCTACATATCATGCTCTGTATCCTCGAGCTTGGACGATATATGATGGTGAACCGGATCCAGAACTTAGAATAGTTTGCCGTCAGATTTCACCTGTTATTCCTCATAATTACAAGGAGAGCAGTTACCCTGTCTCAGTTTTTACTTTCACGCTGCATAATTCTGGAAAAACAGATGCTGACGTCAGTTTGCTTTTCACCTGGGCAAATTCAGTTGGAGGGCTTTCTGAGTATTCTGGTAACCATATCAATTCGAGAACAAAGAAGAAAGATGGCGTGCATACTGTGCTTCTACATCACAAGACAGCAAGTGGATTTCCCCCTGTGACGTATGCTATTGCCGCTCAAGAGGGCAATGGGGTTCATGTCTCAGATTGCCCTTGTTTTGTAATATCTGGTAATTCCCAGGGTATATCAGCCAAGGATATGTGGCTTGAGATCAAAGAGCATGGATCGTTTGATCGGCTTAAATTTGCTGATATGTCAATGCCCTCTGAAGTGGGTTCGTCCATTGGTGCGGCCGTTTCAGCTTCAGTTACAGTTTCATCTGATTCTGTTCGTACTGTGACATTTTCGTTGTCATGGGACTGTCCTGAAGTGAACTTTTGTAGAGGAAAAACTTATCACAGGCGTTACACAAAATTCTATGGTAACCTTGGTGATGCTGCTGCCGATATTGCACGTGATGCTATACTAGAACATCATCACTGGGAATCTCAAATTGATGCTTGGCAAAGACCTGTTCTTGAAGACAAGAGGTTTCCAAAATGGTATCCTGTTACTCTCTTTAATGAGCTTTATTATCTAAATGCAGGAGGGACAATCTGGACAGATGGGTCGCTTCCAATTCAGAGTTTAGTAAGCATAGGGGAAAGAGGATTTTGCCTTGATCAATGCAGATCTGGTGATAGTGTAATTTATGAGTCCCATGAAAATGACACTGCTAATGATATTCTTGGGAGGATGACTTCAAGACTTGATGAGTTACGTGATTCAGTCGCGTCAAATTCTGCATTTGGAGTAAATCTGCTTCAAAAGGGGGAGGAGAATGTTGGGCAGTTCCTTTACCTTGAAGGGGTTGAATACACAATGTGGAATACCTATGACGTTCATTTTTACTCCTCTTTTGCAATAATAATGCTATTTCCAAAACTCGAACTCAGCATTCAACGAGATTTTGCAGCAGCAGTGATGATGCATGATCCCAGCAAGATGCAACTTCTTGATAATGGAAAATGGGAAGCAAGAAATGTTCTTGGAGCTGTTCCTCATGACATCGGAGTTAATGATCCATGGTTTGAAGTGAATGGATATAATCTATATAATACAGATAGGTGGAAGGACTTGAATCCAAAATTTGTCCTCCAAATTTATAGGGATGTTGTTGCCACAGGGGATATGAAATTTGCAAAGGCTGTCTGGCCCCCTGTCTACCTTGCCATAGCTTATATGGATCAGTTCGATCGAGATGGTGATGGGATGATTGAGAATGATGGGTTCCCCGATCAGACTTATGATACGTGGTCTGTCTCTGGTGTTAGTGCATATAGTGGTGGCTTATGGGTGGCAGCGTTGCAGGCTGCTTCAGCCCTGGCTCGTGTAGCCGATGAAAAGGATGCTGAGCACTATTTTTGGTTCAAATTTCAGAAGGCAAAACGTGCCTATGAGAAATTGTGGAATGGTTCTTACTTCAACTATGACAGTAGTGGCGGGAGTTCAAGTTCATCCATTCAAGCTGATCAGTTGGCTGGACAATGGTATATATGA

Coding sequence (CDS)

ATGGAGAATTTTCCATTTCCGCCACTGGAGCTCTCATTAAGGCTTTCTCTTCTTCTTCTTCTTCTTCAATTCCACTTCCCTTTTTCCTCATCATCCTTATCATGCTTTATGGATATAACTTCACTTATAACTGTATCATTTTTTCCCCATGTTGACAGAAATATGTCAGAAGGCAAGAAGTTGGGAAATGGCTTGGTTGAGCAAGATGAGGACATCAATAAATCTCTAACTGAAAAGGTTAAATGGTGCATTCAATCATTTTTTATGACTGGCATTGATCCAGGGCAAACTCCATCACTCACCTGGCAAAGAAAATTAAACAGGGAAGGAACTTCACTTTGGCAGTTCAGGTTGCATCTGAAAGAGATAATTCATCTGGCTCCAGTAGGGTATAGGCTATTCCGCTATATACGAGAAGAATCTGTCAAAGGAAGGGGGGCTATGATAAATCCTTTTATCAGACGTCGTATAACTGATACTCATGCCATTCCTCTAGGTGGTCTTGGATCAGGAAGCATTGGAAGAAGTTACCGAGGTGAATTTCAACGATGGCAACTATTCCCTAGAAAATGTGAAGATAAGCCAATTTTAGCAAATCAATTTTCTGTTTTCGTCTCACGACCGAACGCTGAGAAGTATTCTACTGTACTATGTGCACAGAGCCCTGAAACTCAGAGGGAAGTTGAATCATCAGGCATTGGATCTTGGGATTGGAATTTGAAGGGTCATAGCTCTACATATCATGCTCTGTATCCTCGAGCTTGGACGATATATGATGGTGAACCGGATCCAGAACTTAGAATAGTTTGCCGTCAGATTTCACCTGTTATTCCTCATAATTACAAGGAGAGCAGTTACCCTGTCTCAGTTTTTACTTTCACGCTGCATAATTCTGGAAAAACAGATGCTGACGTCAGTTTGCTTTTCACCTGGGCAAATTCAGTTGGAGGGCTTTCTGAGTATTCTGGTAACCATATCAATTCGAGAACAAAGAAGAAAGATGGCGTGCATACTGTGCTTCTACATCACAAGACAGCAAGTGGATTTCCCCCTGTGACGTATGCTATTGCCGCTCAAGAGGGCAATGGGGTTCATGTCTCAGATTGCCCTTGTTTTGTAATATCTGGTAATTCCCAGGGTATATCAGCCAAGGATATGTGGCTTGAGATCAAAGAGCATGGATCGTTTGATCGGCTTAAATTTGCTGATATGTCAATGCCCTCTGAAGTGGGTTCGTCCATTGGTGCGGCCGTTTCAGCTTCAGTTACAGTTTCATCTGATTCTGTTCGTACTGTGACATTTTCGTTGTCATGGGACTGTCCTGAAGTGAACTTTTGTAGAGGAAAAACTTATCACAGGCGTTACACAAAATTCTATGGTAACCTTGGTGATGCTGCTGCCGATATTGCACGTGATGCTATACTAGAACATCATCACTGGGAATCTCAAATTGATGCTTGGCAAAGACCTGTTCTTGAAGACAAGAGGTTTCCAAAATGGTATCCTGTTACTCTCTTTAATGAGCTTTATTATCTAAATGCAGGAGGGACAATCTGGACAGATGGGTCGCTTCCAATTCAGAGTTTAGTAAGCATAGGGGAAAGAGGATTTTGCCTTGATCAATGCAGATCTGGTGATAGTGTAATTTATGAGTCCCATGAAAATGACACTGCTAATGATATTCTTGGGAGGATGACTTCAAGACTTGATGAGTTACGTGATTCAGTCGCGTCAAATTCTGCATTTGGAGTAAATCTGCTTCAAAAGGGGGAGGAGAATGTTGGGCAGTTCCTTTACCTTGAAGGGGTTGAATACACAATGTGGAATACCTATGACGTTCATTTTTACTCCTCTTTTGCAATAATAATGCTATTTCCAAAACTCGAACTCAGCATTCAACGAGATTTTGCAGCAGCAGTGATGATGCATGATCCCAGCAAGATGCAACTTCTTGATAATGGAAAATGGGAAGCAAGAAATGTTCTTGGAGCTGTTCCTCATGACATCGGAGTTAATGATCCATGGTTTGAAGTGAATGGATATAATCTATATAATACAGATAGGTGGAAGGACTTGAATCCAAAATTTGTCCTCCAAATTTATAGGGATGTTGTTGCCACAGGGGATATGAAATTTGCAAAGGCTGTCTGGCCCCCTGTCTACCTTGCCATAGCTTATATGGATCAGTTCGATCGAGATGGTGATGGGATGATTGAGAATGATGGGTTCCCCGATCAGACTTATGATACGTGGTCTGTCTCTGGTGTTAGTGCATATAGTGGTGGCTTATGGGTGGCAGCGTTGCAGGCTGCTTCAGCCCTGGCTCGTGTAGCCGATGAAAAGGATGCTGAGCACTATTTTTGGTTCAAATTTCAGAAGGCAAAACGTGCCTATGAGAAATTGTGGAATGGTTCTTACTTCAACTATGACAGTAGTGGCGGGAGTTCAAGTTCATCCATTCAAGCTGATCAGTTGGCTGGACAATGGTATATATGA

Protein sequence

MENFPFPPLELSLRLSLLLLLLQFHFPFSSSSLSCFMDITSLITVSFFPHVDRNMSEGKKLGNGLVEQDEDINKSLTEKVKWCIQSFFMTGIDPGQTPSLTWQRKLNREGTSLWQFRLHLKEIIHLAPVGYRLFRYIREESVKGRGAMINPFIRRRITDTHAIPLGGLGSGSIGRSYRGEFQRWQLFPRKCEDKPILANQFSVFVSRPNAEKYSTVLCAQSPETQREVESSGIGSWDWNLKGHSSTYHALYPRAWTIYDGEPDPELRIVCRQISPVIPHNYKESSYPVSVFTFTLHNSGKTDADVSLLFTWANSVGGLSEYSGNHINSRTKKKDGVHTVLLHHKTASGFPPVTYAIAAQEGNGVHVSDCPCFVISGNSQGISAKDMWLEIKEHGSFDRLKFADMSMPSEVGSSIGAAVSASVTVSSDSVRTVTFSLSWDCPEVNFCRGKTYHRRYTKFYGNLGDAAADIARDAILEHHHWESQIDAWQRPVLEDKRFPKWYPVTLFNELYYLNAGGTIWTDGSLPIQSLVSIGERGFCLDQCRSGDSVIYESHENDTANDILGRMTSRLDELRDSVASNSAFGVNLLQKGEENVGQFLYLEGVEYTMWNTYDVHFYSSFAIIMLFPKLELSIQRDFAAAVMMHDPSKMQLLDNGKWEARNVLGAVPHDIGVNDPWFEVNGYNLYNTDRWKDLNPKFVLQIYRDVVATGDMKFAKAVWPPVYLAIAYMDQFDRDGDGMIENDGFPDQTYDTWSVSGVSAYSGGLWVAALQAASALARVADEKDAEHYFWFKFQKAKRAYEKLWNGSYFNYDSSGGSSSSSIQADQLAGQWYI*
BLAST of Csa5G011660 vs. Swiss-Prot
Match: GBA2_RAT (Non-lysosomal glucosylceramidase OS=Rattus norvegicus GN=Gba2 PE=2 SV=2)

HSP 1 Score: 260.8 bits (665), Expect = 5.2e-68
Identity = 143/407 (35.14%), Postives = 222/407 (54.55%), Query Frame = 1

Query: 120 LKEIIHLAPVGYRLFRY-IREESVKGRGAMINPFIRRRITDTHAIPLGGLGSGSIGRSYR 179
           L  ++    +G R  ++  R+  V+ +   I+ F    +   +  PLGG+G G+I R +R
Sbjct: 99  LSNLVKHFGMGLRYLKWWYRKTQVEKKTPFIDMFNSVPLRQIYGCPLGGIGGGTITRGWR 158

Query: 180 GEFQRWQLFPRKCEDKPILANQFSVFVSRPNAEKYSTVLCAQSPETQREVESSGIGSWDW 239
           G+F RWQL P   + + ++A+QF V + R     Y  VL  + P   R        SW+W
Sbjct: 159 GQFCRWQLNPGMYQHQTVIADQFIVCLRRDGRTVYQQVLSLELPSVLR--------SWNW 218

Query: 240 NLKGHSSTYHALYPRAWTIYDGEPDPELRIVCRQISPVIPHNYKESSYPVSVFTFTLHNS 299
            L G+ + YHALYPRAWT+Y   P   + + CRQI+P++PH+Y++SS PV VF + + N 
Sbjct: 219 GLCGYFAFYHALYPRAWTVYQ-LPGQNVTLTCRQITPILPHDYQDSSLPVGVFVWDVENE 278

Query: 300 GKTDADVSLLFTWANSVGGLSEYSGNHINSRTK-KKDG--VHTVLLHHKTASGFPPVTYA 359
           G    DVS++F+  N +GG  + +G   N   + ++DG  V  +LLHH T     P T A
Sbjct: 279 GDETLDVSIMFSMRNGLGGEDDAAGGLWNEPFRLEQDGTTVQGLLLHHPTPPN--PYTMA 338

Query: 360 IAAQEGNGVHVSDCPCFVISGNSQGISAKDMWLEIKEHGSFDRLKFADMSMPSEVGSSIG 419
           +AA+     H +D      +      + + +W ++ + G  D    A  S P++ G  + 
Sbjct: 339 VAAR-----HTADTTVTYTTAFDPDSTGQQVWQDLLQDGQLDSP--AGQSTPTQRGEGVA 398

Query: 420 AAVSASVTVSSDSVRTVTFSLSWDCPEVNF-CRGKTYHRRYTKFYGNLGDAAADIARDAI 479
            AV AS  +       + FSL+WD P + F  +G+ ++RRYT+F+G+ GD A  ++  A+
Sbjct: 399 GAVCASSKLLPRGRCCLEFSLAWDMPRIMFGAKGQVHYRRYTRFFGSDGDVAPALSHYAL 458

Query: 480 LEHHHWESQIDAWQRPVLEDKRFPKWYPVTLFNELYYLNAGGTIWTD 522
            ++  WE+ I AWQ PVL+D+  P WY   LFNELY+L  GGT+W +
Sbjct: 459 CQYAGWENSISAWQNPVLDDRSLPAWYKSALFNELYFLADGGTVWLE 487


HSP 2 Score: 235.7 bits (600), Expect = 1.8e-60
Identity = 116/243 (47.74%), Postives = 153/243 (62.96%), Query Frame = 1

Query: 592 ENVGQFLYLEGVEYTMWNTYDVHFYSSFAIIMLFPKLELSIQRDFAAAVMMHDPSKMQLL 651
           ++ G+F YLEG EY M+NTYDVHFY+SFA++ML+PKLELS+Q D A A    D ++ + L
Sbjct: 509 QDYGRFGYLEGQEYRMYNTYDVHFYASFALVMLWPKLELSLQYDMALATFKEDLTRRRYL 568

Query: 652 DNGKWEARNVLGAVPHDIGVND--PWFEVNGYNLYNTDRWKDLNPKFVLQIYRDVVATGD 711
            +G          +PHDIG  D  PW  VN Y +++T  WKDLN KFVLQ+YRD   TGD
Sbjct: 569 MSGVVAPVKRRNVIPHDIGDPDDEPWLRVNAYLIHDTADWKDLNLKFVLQVYRDYYLTGD 628

Query: 712 MKFAKAVWPPVYLAIAYMDQFDRDGDGMIENDGFPDQTYDTWSVSGVSAYSGGLWVAALQ 771
             F K +WP     +    +FD+D DG+IEN G+ DQTYD W  +G SAY GGLW+AA+ 
Sbjct: 629 QGFLKDMWPVCLAVMESEMKFDKDQDGLIENGGYADQTYDGWVTTGPSAYCGGLWLAAVA 688

Query: 772 AASALARVADEKDAEHYFWFKFQKAKRAYEK-LWNGSYFNYDSSGGSSSSSIQADQLAGQ 831
               +A +   +D +  F     + + AYE+ LWNG Y+NYDSS    S S+ +DQ AGQ
Sbjct: 689 VMVQMAVLCGAQDVQDKFSSILCRGREAYERLLWNGRYYNYDSSSQPQSRSVMSDQCAGQ 748

BLAST of Csa5G011660 vs. Swiss-Prot
Match: GBA2_MOUSE (Non-lysosomal glucosylceramidase OS=Mus musculus GN=Gba2 PE=1 SV=2)

HSP 1 Score: 258.1 bits (658), Expect = 3.4e-67
Identity = 146/417 (35.01%), Postives = 223/417 (53.48%), Query Frame = 1

Query: 118 LHLKEIIHLAPVGYRLFRY-IREESVKGRGAMINPFIRRRITDTHAIPLGGLGSGSIGRS 177
           + L  ++    +G R  ++  R+  V+ +   I+      +   +  PLGG+G G+I R 
Sbjct: 97  ISLSNLVKHLGMGLRYLKWWYRKTHVEKKTPFIDMLNSLPLRQIYGCPLGGIGGGTITRG 156

Query: 178 YRGEFQRWQLFPRKCEDKPILANQFSVFVSRPNAEKYSTVLCAQSPETQREVESSGIGSW 237
           +RG+F RWQL P   + + ++A+QF V + R     Y  VL  + P   R        SW
Sbjct: 157 WRGQFCRWQLNPGMYQHQTVIADQFIVCLRRDGRTVYQQVLSLELPNVLR--------SW 216

Query: 238 DWNLKGHSSTYHALYPRAWTIYDGEPDPELRIVCRQISPVIPHNYKESSYPVSVFTFTLH 297
           +W L G+ + YHALYPRAWT+Y   P   + + CRQ++P++PH+Y++SS PV VF + + 
Sbjct: 217 NWGLCGYFAFYHALYPRAWTVYQ-LPGQNVTLTCRQVTPILPHDYQDSSLPVGVFVWDVE 276

Query: 298 NSGKTDADVSLLFTWANSVGGLSEYSGNHINSRTKKKDGVHTV---LLHHKTASGFPPVT 357
           N G    DVS+ F+  N +GG  + +G+  N   + + G  TV   LLHH T     P T
Sbjct: 277 NEGDETLDVSITFSMRNGLGGEDDAAGSLWNEPFRLEQGGTTVQGLLLHHPTPPN--PYT 336

Query: 358 YAIAAQEGNGVHVSDCPCFVISGNSQGISAKDMWLEIKEHGSFDRLKFADMSMPSEVGSS 417
            A+AA+      V+    F  +G  Q +     W ++ + G  D    A  S P++ G  
Sbjct: 337 MAVAARCTADTTVTHTTAFDPNGTGQQV-----WQDLLQDGQLDSP--AGQSTPTQKGEG 396

Query: 418 IGAAVSASVTVSSDSVRTVTFSLSWDCPEVNF-CRGKTYHRRYTKFYGNLGDAAADIARD 477
           I  AV  S  +   S   + FSL+WD P++ F  + + ++RRYT+F+G+ GD A  ++  
Sbjct: 397 IAGAVCVSSKLLPRSRCCLEFSLAWDMPKIMFGAKSQVHYRRYTRFFGSDGDVAPALSHY 456

Query: 478 AILEHHHWESQIDAWQRPVLEDKRFPKWYPVTLFNELYYLNAGGTIWTDGSLPIQSL 530
           A+  +  WE +I AWQ PVL+D+  P WY   LFNELY+L  GGT+W +  +P  SL
Sbjct: 457 ALCHYADWEDRISAWQNPVLDDRTLPAWYKSALFNELYFLADGGTVWLE--VPADSL 493


HSP 2 Score: 238.4 bits (607), Expect = 2.8e-61
Identity = 120/261 (45.98%), Postives = 160/261 (61.30%), Query Frame = 1

Query: 574 DSVASNSAFGVNLLQKGEENVGQFLYLEGVEYTMWNTYDVHFYSSFAIIMLFPKLELSIQ 633
           DS+       +  L+   ++ G+F YLEG EY M+NTYDVHFY+SFA++ML+PKLELS+Q
Sbjct: 491 DSLPEGLGGSMRQLRSTLQDYGRFGYLEGQEYRMYNTYDVHFYASFALVMLWPKLELSLQ 550

Query: 634 RDFAAAVMMHDPSKMQLLDNGKWEARNVLGAVPHDIGVND--PWFEVNGYNLYNTDRWKD 693
            D A A +  D ++ + L +G          +PHDIG  D  PW  VN Y +++T  WKD
Sbjct: 551 YDMALATLKEDLTRRRYLMSGVVAPVKRRNVIPHDIGDPDDEPWLRVNAYLIHDTADWKD 610

Query: 694 LNPKFVLQIYRDVVATGDMKFAKAVWPPVYLAIAYMDQFDRDGDGMIENDGFPDQTYDTW 753
           LN KFVLQIYRD   TGD  F + +WP     +    +FD+D DG+IEN G+ DQTYD W
Sbjct: 611 LNLKFVLQIYRDYYLTGDQGFLEDMWPVCLAVMESEMKFDKDQDGLIENGGYADQTYDAW 670

Query: 754 SVSGVSAYSGGLWVAALQAASALARVADEKDAEHYFWFKFQKAKRAYEK-LWNGSYFNYD 813
             +G SAY GGLW+AA+     +A +   +D +  F     + + AYE+ LWNG Y+NYD
Sbjct: 671 VTTGPSAYCGGLWLAAVAVMVQMAVLCGAQDVQERFASILCRGREAYERLLWNGRYYNYD 730

Query: 814 SSGGSSSSSIQADQLAGQWYI 832
           SS    S SI +DQ AGQW++
Sbjct: 731 SSSHPQSRSIMSDQCAGQWFL 751

BLAST of Csa5G011660 vs. Swiss-Prot
Match: GBA2_HUMAN (Non-lysosomal glucosylceramidase OS=Homo sapiens GN=GBA2 PE=1 SV=2)

HSP 1 Score: 254.6 bits (649), Expect = 3.7e-66
Identity = 148/435 (34.02%), Postives = 221/435 (50.80%), Query Frame = 1

Query: 96  QTPSLTWQRKLNREGTS----LWQFRLHLKEIIHLAPVGYRLFRY-IREESVKGRGAMIN 155
           Q P   W+  L  E T          + L  +I    +G R  ++  R+  V+ +   I+
Sbjct: 80  QVPPFGWRICLAHEFTEKRKPFQANNVSLSNMIKHIGMGLRYLQWWYRKTHVEKKTPFID 139

Query: 156 PFIRRRITDTHAIPLGGLGSGSIGRSYRGEFQRWQLFPRKCEDKPILANQFSVFVSRPNA 215
                 +   +  PLGG+G G+I R +RG+F RWQL P   + + ++A+QF+V + R   
Sbjct: 140 MINSVPLRQIYGCPLGGIGGGTITRGWRGQFCRWQLNPGMYQHRTVIADQFTVCLRREGQ 199

Query: 216 EKYSTVLCAQSPETQREVESSGIGSWDWNLKGHSSTYHALYPRAWTIYDGEPDPELRIVC 275
             Y  VL  + P   R        SW+W L G+ + YHALYPRAWT+Y   P   + + C
Sbjct: 200 TVYQQVLSLERPSVLR--------SWNWGLCGYFAFYHALYPRAWTVYQ-LPGQNVTLTC 259

Query: 276 RQISPVIPHNYKESSYPVSVFTFTLHNSGKTDADVSLLFTWANSVGGLSEYSGNHINSR- 335
           RQI+P++PH+Y++SS PV VF + + N G    DVS++F+  N +GG  +  G   N   
Sbjct: 260 RQITPILPHDYQDSSLPVGVFVWDVENEGDEALDVSIMFSMRNGLGGGDDAPGGLWNEPF 319

Query: 336 --TKKKDGVHTVLLHHKTASGFPPVTYAIAAQEGNGVHVSDCPCFVISGNSQGISAKDMW 395
              +  + V  +LLHH T     P T A+AA+      V+    F      Q +     W
Sbjct: 320 CLERSGETVRGLLLHHPTLPN--PYTMAVAARVTAATTVTHITAFDPDSTGQQV-----W 379

Query: 396 LEIKEHGSFDRLKFADMSMPSEVGSSIGAAVSASVTVSSDSVRTVTFSLSWDCPEVNF-C 455
            ++ + G  D       S P++ G  I  AV  S  +       + FSL+WD P + F  
Sbjct: 380 QDLLQDGQLDSP--TGQSTPTQKGVGIAGAVCVSSKLRPRGQCRLEFSLAWDMPRIMFGA 439

Query: 456 RGKTYHRRYTKFYGNLGDAAADIARDAILEHHHWESQIDAWQRPVLEDKRFPKWYPVTLF 515
           +G+ ++RRYT+F+G  GDAA  ++  A+  +  WE +I AWQ PVL+D+  P WY   LF
Sbjct: 440 KGQVHYRRYTRFFGQDGDAAPALSHYALCRYAEWEERISAWQSPVLDDRSLPAWYKSALF 496

Query: 516 NELYYLNAGGTIWTD 522
           NELY+L  GGT+W +
Sbjct: 500 NELYFLADGGTVWLE 496


HSP 2 Score: 241.5 bits (615), Expect = 3.3e-62
Identity = 122/266 (45.86%), Postives = 162/266 (60.90%), Query Frame = 1

Query: 569 LDELRDSVASNSAFGVNLLQKGEENVGQFLYLEGVEYTMWNTYDVHFYSSFAIIMLFPKL 628
           L+ L DS+       +  L+    + G+F YLEG EY M+NTYDVHFY+SFA+IML+PKL
Sbjct: 495 LEVLEDSLPEELGRNMCHLRPTLRDYGRFGYLEGQEYRMYNTYDVHFYASFALIMLWPKL 554

Query: 629 ELSIQRDFAAAVMMHDPSKMQLLDNGKWEARNVLGAVPHDIGVND--PWFEVNGYNLYNT 688
           ELS+Q D A A +  D ++ + L +G          +PHDIG  D  PW  VN Y +++T
Sbjct: 555 ELSLQYDMALATLREDLTRRRYLMSGVMAPVKRRNVIPHDIGDPDDEPWLRVNAYLIHDT 614

Query: 689 DRWKDLNPKFVLQIYRDVVATGDMKFAKAVWPPVYLAIAYMDQFDRDGDGMIENDGFPDQ 748
             WKDLN KFVLQ+YRD   TGD  F K +WP     +    +FD+D DG+IEN G+ DQ
Sbjct: 615 ADWKDLNLKFVLQVYRDYYLTGDQNFLKDMWPVCLAVMESEMKFDKDHDGLIENGGYADQ 674

Query: 749 TYDTWSVSGVSAYSGGLWVAALQAASALARVADEKDAEHYFWFKFQKAKRAYEK-LWNGS 808
           TYD W  +G SAY GGLW+AA+     +A +   +D +  F     + + AYE+ LWNG 
Sbjct: 675 TYDGWVTTGPSAYCGGLWLAAVAVMVQMAALCGAQDIQDKFSSILSRGQEAYERLLWNGR 734

Query: 809 YFNYDSSGGSSSSSIQADQLAGQWYI 832
           Y+NYDSS    S S+ +DQ AGQW++
Sbjct: 735 YYNYDSSSRPQSRSVMSDQCAGQWFL 760

BLAST of Csa5G011660 vs. Swiss-Prot
Match: C3390_DROME (Non-lysosomal glucosylceramidase OS=Drosophila melanogaster GN=CG33090 PE=1 SV=1)

HSP 1 Score: 223.0 bits (567), Expect = 1.2e-56
Identity = 129/425 (30.35%), Postives = 211/425 (49.65%), Query Frame = 1

Query: 117 RLHLKEIIHLAPVGYRLFRYIREESVKGRGAMINPFIRRRITDTHAIPLGGLGSGSIGRS 176
           R  +++ + + P+  R   Y  + S +GR   ++ +        + +P+GG+G G+IGR 
Sbjct: 72  RASIRQTLPMVPLVCRYAAYYWKVSREGRRVYMDYYYMENGKQIYGVPIGGIGGGTIGRG 131

Query: 177 YRGEFQRWQLFPRKCEDKPILANQFSVFVSRPNA--------EKYSTVLCAQSPETQREV 236
           Y GEF R+Q+ P   E   +LANQF V +  P           K ST      P+   + 
Sbjct: 132 YAGEFCRFQMRPGIYEYNVVLANQFIVTIKDPKGCTIFQSLLSKCSTRDKTSDPDGDPDG 191

Query: 237 ESSG-------------IGSWDWNLKGHSSTYHALYPRAWTIYDGEPDPELRIVCRQISP 296
           E +              + +W  N++    +Y  LYPR+WT YD      +R+ CRQ+SP
Sbjct: 192 ERTKCQLPNCSSRAKQPLSAWHSNIEDTRCSYTGLYPRSWTEYDLS-HYGVRLTCRQVSP 251

Query: 297 VIPHNYKESSYPVSVFTFTLHNSGKTDADVSLLFTWANSVGGLSEYSGNHINSRTKKKDG 356
           VIPH Y+ESS P +VF +++ N    +  VS+ FT+ N  G   + +     S+   +  
Sbjct: 252 VIPHEYRESSLPCAVFVWSVENVCDQERKVSITFTFKNGTGNKKQDAEGGAESQLISEGN 311

Query: 357 VHTVLLHHKTASGFPPVTYAIAAQEGNGVHVSDCPCFVISGNSQGISAKDMWLEIKEHGS 416
              V +  K +    P +Y +A +    + ++ CP F  +GN +      +W ++KEHG 
Sbjct: 312 AKGVSIRQKISE--MPCSYNLACRVLPEISITRCPQFDPAGNGE-----QLWAQLKEHGQ 371

Query: 417 FDRLKFADMSMPSEVGSSIGAAVSASVTVSSDSVRTVTFSLSWDCPEVNFCRG-KTYHRR 476
                 ++     ++G ++   V+     S D    + F L+WD P++ F R  +T+ R 
Sbjct: 372 LSEHPTSEALKTKDIGVAVCGQVALKPMASHD----LEFVLAWDMPKIQFPRKMQTHTRY 431

Query: 477 YTKFYGNLGDAAADIARDAILEHHHWESQIDAWQRPVLEDKRFPKWYPVTLFNELYYLNA 520
           YTK++ + GD+   I   A+ ++  WE  IDAWQRP+L D+  P WY   +FN+LY+++ 
Sbjct: 432 YTKYFDDSGDSGPRICEYALRQYSTWERLIDAWQRPILNDETLPDWYKCAIFNQLYFISD 484


HSP 2 Score: 112.8 bits (281), Expect = 1.8e-23
Identity = 58/137 (42.34%), Postives = 85/137 (62.04%), Query Frame = 1

Query: 595 GQFLYLEGVEYTMWNTYDVHFYSSFAIIMLFPKLELSIQRDFAAAVMMHDPSKMQLLDNG 654
           G+F YLEG EY M+NTYDVHFY+S A+  L+P L++S+Q DF  A+        ++L +G
Sbjct: 505 GRFGYLEGHEYRMYNTYDVHFYASPALAHLWPNLQVSLQYDFKDAIAAELNDTRKMLYDG 564

Query: 655 KWEARNVLGAVPHDIGVND--PWFEVNGYNLYNTDRWKDLNPKFVLQIYRDVVATGDMKF 714
           K   R V   VPHD+G  D  P+  +N YN+++ + WKDLN KFVLQ+YRD     ++  
Sbjct: 565 KVMPRKVKNCVPHDLGDPDEEPFTLINCYNIHDVNDWKDLNTKFVLQVYRDYYVLNELAQ 624

Query: 715 AKAVWPPVYLAIAYMDQ 730
           A++     + +I ++D+
Sbjct: 625 AQSDNASKFSSIEFIDK 641


HSP 3 Score: 106.7 bits (265), Expect = 1.3e-21
Identity = 54/121 (44.63%), Postives = 74/121 (61.16%), Query Frame = 1

Query: 712 FAKAVWPPVYLAIAYMDQFDRDGDGMIENDGFPDQTYDTWSVSGVSAYSGGLWVAALQAA 771
           + KA++      +    ++D+D DG+IEN   PDQTYD+W + G SAY  GLW+AALQA 
Sbjct: 687 YLKAMYASCKAIMERTIEYDKDNDGLIENTKMPDQTYDSWVMDGPSAYCSGLWLAALQAM 746

Query: 772 SALARVADEKDAEHYFWFKFQKAKRAY-EKLWNGSYFNYDSSGGSSSSSIQADQLAGQWY 831
           SA+A + D+ +    +    +K KR+  EKLWNGSY+ +D S  S   +I ADQL G WY
Sbjct: 747 SAMATILDQPNDCLRYQDILEKGKRSLEEKLWNGSYYRFDLS-HSHRDTIMADQLCGHWY 806

BLAST of Csa5G011660 vs. TrEMBL
Match: A0A0A0KPF7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G011660 PE=4 SV=1)

HSP 1 Score: 1719.5 bits (4452), Expect = 0.0e+00
Identity = 831/831 (100.00%), Postives = 831/831 (100.00%), Query Frame = 1

Query: 1   MENFPFPPLELSLRLSLLLLLLQFHFPFSSSSLSCFMDITSLITVSFFPHVDRNMSEGKK 60
           MENFPFPPLELSLRLSLLLLLLQFHFPFSSSSLSCFMDITSLITVSFFPHVDRNMSEGKK
Sbjct: 1   MENFPFPPLELSLRLSLLLLLLQFHFPFSSSSLSCFMDITSLITVSFFPHVDRNMSEGKK 60

Query: 61  LGNGLVEQDEDINKSLTEKVKWCIQSFFMTGIDPGQTPSLTWQRKLNREGTSLWQFRLHL 120
           LGNGLVEQDEDINKSLTEKVKWCIQSFFMTGIDPGQTPSLTWQRKLNREGTSLWQFRLHL
Sbjct: 61  LGNGLVEQDEDINKSLTEKVKWCIQSFFMTGIDPGQTPSLTWQRKLNREGTSLWQFRLHL 120

Query: 121 KEIIHLAPVGYRLFRYIREESVKGRGAMINPFIRRRITDTHAIPLGGLGSGSIGRSYRGE 180
           KEIIHLAPVGYRLFRYIREESVKGRGAMINPFIRRRITDTHAIPLGGLGSGSIGRSYRGE
Sbjct: 121 KEIIHLAPVGYRLFRYIREESVKGRGAMINPFIRRRITDTHAIPLGGLGSGSIGRSYRGE 180

Query: 181 FQRWQLFPRKCEDKPILANQFSVFVSRPNAEKYSTVLCAQSPETQREVESSGIGSWDWNL 240
           FQRWQLFPRKCEDKPILANQFSVFVSRPNAEKYSTVLCAQSPETQREVESSGIGSWDWNL
Sbjct: 181 FQRWQLFPRKCEDKPILANQFSVFVSRPNAEKYSTVLCAQSPETQREVESSGIGSWDWNL 240

Query: 241 KGHSSTYHALYPRAWTIYDGEPDPELRIVCRQISPVIPHNYKESSYPVSVFTFTLHNSGK 300
           KGHSSTYHALYPRAWTIYDGEPDPELRIVCRQISPVIPHNYKESSYPVSVFTFTLHNSGK
Sbjct: 241 KGHSSTYHALYPRAWTIYDGEPDPELRIVCRQISPVIPHNYKESSYPVSVFTFTLHNSGK 300

Query: 301 TDADVSLLFTWANSVGGLSEYSGNHINSRTKKKDGVHTVLLHHKTASGFPPVTYAIAAQE 360
           TDADVSLLFTWANSVGGLSEYSGNHINSRTKKKDGVHTVLLHHKTASGFPPVTYAIAAQE
Sbjct: 301 TDADVSLLFTWANSVGGLSEYSGNHINSRTKKKDGVHTVLLHHKTASGFPPVTYAIAAQE 360

Query: 361 GNGVHVSDCPCFVISGNSQGISAKDMWLEIKEHGSFDRLKFADMSMPSEVGSSIGAAVSA 420
           GNGVHVSDCPCFVISGNSQGISAKDMWLEIKEHGSFDRLKFADMSMPSEVGSSIGAAVSA
Sbjct: 361 GNGVHVSDCPCFVISGNSQGISAKDMWLEIKEHGSFDRLKFADMSMPSEVGSSIGAAVSA 420

Query: 421 SVTVSSDSVRTVTFSLSWDCPEVNFCRGKTYHRRYTKFYGNLGDAAADIARDAILEHHHW 480
           SVTVSSDSVRTVTFSLSWDCPEVNFCRGKTYHRRYTKFYGNLGDAAADIARDAILEHHHW
Sbjct: 421 SVTVSSDSVRTVTFSLSWDCPEVNFCRGKTYHRRYTKFYGNLGDAAADIARDAILEHHHW 480

Query: 481 ESQIDAWQRPVLEDKRFPKWYPVTLFNELYYLNAGGTIWTDGSLPIQSLVSIGERGFCLD 540
           ESQIDAWQRPVLEDKRFPKWYPVTLFNELYYLNAGGTIWTDGSLPIQSLVSIGERGFCLD
Sbjct: 481 ESQIDAWQRPVLEDKRFPKWYPVTLFNELYYLNAGGTIWTDGSLPIQSLVSIGERGFCLD 540

Query: 541 QCRSGDSVIYESHENDTANDILGRMTSRLDELRDSVASNSAFGVNLLQKGEENVGQFLYL 600
           QCRSGDSVIYESHENDTANDILGRMTSRLDELRDSVASNSAFGVNLLQKGEENVGQFLYL
Sbjct: 541 QCRSGDSVIYESHENDTANDILGRMTSRLDELRDSVASNSAFGVNLLQKGEENVGQFLYL 600

Query: 601 EGVEYTMWNTYDVHFYSSFAIIMLFPKLELSIQRDFAAAVMMHDPSKMQLLDNGKWEARN 660
           EGVEYTMWNTYDVHFYSSFAIIMLFPKLELSIQRDFAAAVMMHDPSKMQLLDNGKWEARN
Sbjct: 601 EGVEYTMWNTYDVHFYSSFAIIMLFPKLELSIQRDFAAAVMMHDPSKMQLLDNGKWEARN 660

Query: 661 VLGAVPHDIGVNDPWFEVNGYNLYNTDRWKDLNPKFVLQIYRDVVATGDMKFAKAVWPPV 720
           VLGAVPHDIGVNDPWFEVNGYNLYNTDRWKDLNPKFVLQIYRDVVATGDMKFAKAVWPPV
Sbjct: 661 VLGAVPHDIGVNDPWFEVNGYNLYNTDRWKDLNPKFVLQIYRDVVATGDMKFAKAVWPPV 720

Query: 721 YLAIAYMDQFDRDGDGMIENDGFPDQTYDTWSVSGVSAYSGGLWVAALQAASALARVADE 780
           YLAIAYMDQFDRDGDGMIENDGFPDQTYDTWSVSGVSAYSGGLWVAALQAASALARVADE
Sbjct: 721 YLAIAYMDQFDRDGDGMIENDGFPDQTYDTWSVSGVSAYSGGLWVAALQAASALARVADE 780

Query: 781 KDAEHYFWFKFQKAKRAYEKLWNGSYFNYDSSGGSSSSSIQADQLAGQWYI 832
           KDAEHYFWFKFQKAKRAYEKLWNGSYFNYDSSGGSSSSSIQADQLAGQWYI
Sbjct: 781 KDAEHYFWFKFQKAKRAYEKLWNGSYFNYDSSGGSSSSSIQADQLAGQWYI 831

BLAST of Csa5G011660 vs. TrEMBL
Match: B9SS77_RICCO (Non-lysosomal glucosylceramidase OS=Ricinus communis GN=RCOM_0618840 PE=3 SV=1)

HSP 1 Score: 1197.6 bits (3097), Expect = 0.0e+00
Identity = 582/777 (74.90%), Postives = 650/777 (83.66%), Query Frame = 1

Query: 55  MSEGKKLGNGLVEQDEDINKSLTEKVKWCIQSFFMTGIDPGQTPSLTWQRKLNREGTSLW 114
           MSEG+   NG  ++D + + SL EKV            DPG   SLTWQRKLN E  +L 
Sbjct: 1   MSEGEIPANGC-QEDREPSDSLLEKV------------DPGSPASLTWQRKLNSEDIALS 60

Query: 115 QFRLHLKEIIHLAPVGYRLFRYIREESVKGRGAMINPFIRRRITDTHAIPLGGLGSGSIG 174
           QF L  +E   LAPVG RL+R IREE+ KGR ++INPF++R IT  H IPLGG+GSGSIG
Sbjct: 61  QFNLSFQEKFQLAPVGIRLWRLIREETAKGRVSIINPFLKRFITSCHGIPLGGIGSGSIG 120

Query: 175 RSYRGEFQRWQLFPRKCEDKPILANQFSVFVSRPNAEKYSTVLCAQSPETQREVESSGIG 234
           RSY+GEFQRWQLFPR CE+KP+LANQFSVFVSR + EKYS+VLC ++PE   E   SGIG
Sbjct: 121 RSYKGEFQRWQLFPRICEEKPVLANQFSVFVSRSSGEKYSSVLCPRNPEVLMEPAISGIG 180

Query: 235 SWDWNLKGHSSTYHALYPRAWTIYDGEPDPELRIVCRQISPVIPHNYKESSYPVSVFTFT 294
           SWDWNLKG +STYHALYPRAWTIYDGEPDPELRIVCRQISP+IPHNYKESSYPVSVFTFT
Sbjct: 181 SWDWNLKGDNSTYHALYPRAWTIYDGEPDPELRIVCRQISPIIPHNYKESSYPVSVFTFT 240

Query: 295 LHNSGKTDADVSLLFTWANSVGGLSEYSGNHINSRTKKKDGVHTVLLHHKTASGFPPVTY 354
           L+NSGKT ADVSLLFTW NSVGG SEYSG H NS T  +DGVH VLLHHKTA GFPPVT+
Sbjct: 241 LYNSGKTTADVSLLFTWTNSVGGNSEYSGQHFNSTTMMEDGVHAVLLHHKTAEGFPPVTF 300

Query: 355 AIAAQEGNGVHVSDCPCFVISGNSQGISAKDMWLEIKEHGSFDRLKFADMSMPSEVGSSI 414
           AIAAQE N VHVS CP FVISGN QGI+AKDMW E+KEHGSFD LK A  S PSE GSSI
Sbjct: 301 AIAAQETNDVHVSKCPRFVISGNCQGITAKDMWHEVKEHGSFDNLKSAGTSGPSEPGSSI 360

Query: 415 GAAVSASVTVSSDSVRTVTFSLSWDCPEVNFCRGKTYHRRYTKFYGNLGDAAADIARDAI 474
           GAA++ASVT+  D++R+VTFSLSWDCPEV F  G+TYHRRYTKFY   GDAAA IA DAI
Sbjct: 361 GAAIAASVTIPPDAIRSVTFSLSWDCPEVYFMGGRTYHRRYTKFYSTHGDAAARIAHDAI 420

Query: 475 LEHHHWESQIDAWQRPVLEDKRFPKWYPVTLFNELYYLNAGGTIWTDGSLPIQSLVSIGE 534
           LEH  WESQI AWQRP+LEDKR P+WYP+TLFNELYYLN+GGTIWTDGS P  +LVSI  
Sbjct: 421 LEHGLWESQIVAWQRPILEDKRLPEWYPITLFNELYYLNSGGTIWTDGSPPYHNLVSIRG 480

Query: 535 RGFCLDQCRSG-DSVIYESHENDTANDILGRMTSRLDELRDSVASNSAFGVNLLQKGEEN 594
             F LD   +G  S+I  +HENDTA +ILGRMTS L+++   VASNSAFG NLLQ+GEEN
Sbjct: 481 SKFSLDTSGAGLKSIIDVTHENDTAVNILGRMTSALEQIHAHVASNSAFGTNLLQEGEEN 540

Query: 595 VGQFLYLEGVEYTMWNTYDVHFYSSFAIIMLFPKLELSIQRDFAAAVMMHDPSKMQLLDN 654
           +GQFLYLEG+EY MWNTYDVHFYSSFA++MLFPKLELS+QRDFAAAVMMHDPSKMQLL +
Sbjct: 541 IGQFLYLEGIEYHMWNTYDVHFYSSFALVMLFPKLELSVQRDFAAAVMMHDPSKMQLLHD 600

Query: 655 GKWEARNVLGAVPHDIGVNDPWFEVNGYNLYNTDRWKDLNPKFVLQIYRDVVATGDMKFA 714
           G+W  R VLGAVPHDIG+NDPW+EVN Y+LYNTDRWKDLNPKFVLQ+YRDVVATGD KFA
Sbjct: 601 GQWVCRKVLGAVPHDIGINDPWYEVNAYSLYNTDRWKDLNPKFVLQVYRDVVATGDKKFA 660

Query: 715 KAVWPPVYLAIAYMDQFDRDGDGMIENDGFPDQTYDTWSVSGVSAYSGGLWVAALQAASA 774
           +AVWP VY+A+AYMDQFDRDGDGMIENDGFPDQTYDTWSVSGVSAYSGGLWVAALQAASA
Sbjct: 661 EAVWPSVYIAMAYMDQFDRDGDGMIENDGFPDQTYDTWSVSGVSAYSGGLWVAALQAASA 720

Query: 775 LARVADEKDAEHYFWFKFQKAKRAYEKLWNGSYFNYDSSGGSSSSSIQADQLAGQWY 831
           LAR   +K +E YFW +FQKAK  Y+KLWNGSYFNYD+SGG +SSSIQADQLAGQWY
Sbjct: 721 LAREVGDKGSEDYFWARFQKAKDVYDKLWNGSYFNYDNSGGRNSSSIQADQLAGQWY 764

BLAST of Csa5G011660 vs. TrEMBL
Match: M5XQ56_PRUPE (Non-lysosomal glucosylceramidase OS=Prunus persica GN=PRUPE_ppa000954mg PE=3 SV=1)

HSP 1 Score: 1194.9 bits (3090), Expect = 0.0e+00
Identity = 569/777 (73.23%), Postives = 656/777 (84.43%), Query Frame = 1

Query: 55  MSEGKKLGNGLVEQDEDINKSLTEKVKWCIQSFFMTGIDPGQTPSLTWQRKLNREGTSLW 114
           M E K L NG VE+D++ + S  +KV            DPG+  SLTW+RKLN +G    
Sbjct: 1   MLEKKILENGFVERDKEDSDSSFDKV------------DPGKPTSLTWKRKLNSKGNDPL 60

Query: 115 QFRLHLKEIIHLAPVGYRLFRYIREESVKGRGAMINPFIRRRITDTHAIPLGGLGSGSIG 174
            F L LKEII +AP+G RL+R++REE+  GR A INPF++R +T +H +PLGG+G+GSIG
Sbjct: 61  PFTLSLKEIIQMAPIGVRLWRHLREEATNGREAFINPFVKRLLTSSHGVPLGGIGAGSIG 120

Query: 175 RSYRGEFQRWQLFPRKCEDKPILANQFSVFVSRPNAEKYSTVLCAQSPETQREVESSGIG 234
           RSY GEFQRWQLFP K E+KP+LA+QFSVFVSR N EKY TVLC + PE  +E E SGIG
Sbjct: 121 RSYSGEFQRWQLFPGKFEEKPVLADQFSVFVSRTNGEKYCTVLCPRRPEVLKESEVSGIG 180

Query: 235 SWDWNLKGHSSTYHALYPRAWTIYDGEPDPELRIVCRQISPVIPHNYKESSYPVSVFTFT 294
           SWDWNL G +STYHAL+PRAW++Y+GEPDP L+IVCRQISP IPHNYKESS+PVSVFTFT
Sbjct: 181 SWDWNLNGDNSTYHALFPRAWSVYEGEPDPALKIVCRQISPFIPHNYKESSFPVSVFTFT 240

Query: 295 LHNSGKTDADVSLLFTWANSVGGLSEYSGNHINSRTKKKDGVHTVLLHHKTASGFPPVTY 354
           L+NSGKT ADV+LLFTWANSVGGLSE+SG+H NSR   KDGVH VLLHHKTA+G PPVT+
Sbjct: 241 LYNSGKTAADVTLLFTWANSVGGLSEFSGHHFNSRAVIKDGVHGVLLHHKTANGLPPVTF 300

Query: 355 AIAAQEGNGVHVSDCPCFVISGNSQGISAKDMWLEIKEHGSFDRLKFADMSMPSEVGSSI 414
           AIAA+E +G+HVS+CPCFVISG+S+GI+AKDMW EIKEHGSFDRL   + S  SE GSSI
Sbjct: 301 AIAAEETDGIHVSECPCFVISGDSKGITAKDMWTEIKEHGSFDRLNSTETSSDSEPGSSI 360

Query: 415 GAAVSASVTVSSDSVRTVTFSLSWDCPEVNFCRGKTYHRRYTKFYGNLGDAAADIARDAI 474
           GAA++ASVTV  D VRTVTFSL+WDCPEV F  GKTYHRRYTKFYG  GDA A+IA DAI
Sbjct: 361 GAAIAASVTVPPDGVRTVTFSLAWDCPEVKFMGGKTYHRRYTKFYGTHGDAVANIAHDAI 420

Query: 475 LEHHHWESQIDAWQRPVLEDKRFPKWYPVTLFNELYYLNAGGTIWTDGSLPIQSLVSIGE 534
           LEHHHWESQI++WQRPVL+DKR P+WYP+TLFNELYYLN+GGT+WTDGS P+ SL SIG 
Sbjct: 421 LEHHHWESQIESWQRPVLDDKRLPEWYPITLFNELYYLNSGGTVWTDGSPPVHSLTSIGG 480

Query: 535 RGFCLDQCRSG-DSVIYESHENDTANDILGRMTSRLDELRDSVASNSAFGVNLLQKGEEN 594
           R F LD+   G  S+I    +NDTA DILGRMTS L+++   +ASNSAFG NLLQ+GEEN
Sbjct: 481 RKFSLDRSSLGLKSIIDVPPQNDTAIDILGRMTSILEQVHTPIASNSAFGTNLLQEGEEN 540

Query: 595 VGQFLYLEGVEYTMWNTYDVHFYSSFAIIMLFPKLELSIQRDFAAAVMMHDPSKMQLLDN 654
           +GQFLYLEG+EY MWNTYDVHFYSSFA++MLFPKL+LSIQRDFAAAVMMHDPSKM+LL +
Sbjct: 541 IGQFLYLEGIEYQMWNTYDVHFYSSFALVMLFPKLQLSIQRDFAAAVMMHDPSKMRLLHD 600

Query: 655 GKWEARNVLGAVPHDIGVNDPWFEVNGYNLYNTDRWKDLNPKFVLQIYRDVVATGDMKFA 714
           GKW  R VLGAVPHDIG++DPWFEVN YNLYNTDRWKDLNPKFVLQ+YRDVVATGD KFA
Sbjct: 601 GKWVQRKVLGAVPHDIGLHDPWFEVNAYNLYNTDRWKDLNPKFVLQVYRDVVATGDKKFA 660

Query: 715 KAVWPPVYLAIAYMDQFDRDGDGMIENDGFPDQTYDTWSVSGVSAYSGGLWVAALQAASA 774
           +AVWP VY+A+AYM+QFD+DGDGMIENDGFPDQTYDTWSVSGVSAYSGGLW+AALQAASA
Sbjct: 661 QAVWPSVYVAMAYMEQFDKDGDGMIENDGFPDQTYDTWSVSGVSAYSGGLWLAALQAASA 720

Query: 775 LARVADEKDAEHYFWFKFQKAKRAYEKLWNGSYFNYDSSGGSSSSSIQADQLAGQWY 831
           +AR   +K +E YFW KFQKAK  YEKLWNGSYFNYD+SG SSSSSIQADQLAGQWY
Sbjct: 721 MAREVGDKGSEDYFWGKFQKAKVVYEKLWNGSYFNYDNSGQSSSSSIQADQLAGQWY 765

BLAST of Csa5G011660 vs. TrEMBL
Match: D7TGQ9_VITVI (Non-lysosomal glucosylceramidase OS=Vitis vinifera GN=VIT_12s0035g01220 PE=3 SV=1)

HSP 1 Score: 1186.8 bits (3069), Expect = 0.0e+00
Identity = 562/769 (73.08%), Postives = 656/769 (85.31%), Query Frame = 1

Query: 63  NGLVEQDEDINKSLTEKVKWCIQSFFMTGIDPGQTPSLTWQRKLNREGTSLWQFRLHLKE 122
           NGL E + + + S  E++K          +DPG+  SLTWQRKLN +G +  +F+++L+E
Sbjct: 4   NGLEEGEREPSNSSIEEIK----------VDPGKPGSLTWQRKLNSDGNAPVEFKINLRE 63

Query: 123 IIHLAPVGYRLFRYIREESVKGRGAMINPFIRRRITDTHAIPLGGLGSGSIGRSYRGEFQ 182
             HLAP+G+RL+R++REE+ KGRGAMI+PF++R I+ +  +PLGG+G+GSIGRSY+GEFQ
Sbjct: 64  TFHLAPIGFRLWRHVREETAKGRGAMIDPFVKRYISSSQGVPLGGIGAGSIGRSYKGEFQ 123

Query: 183 RWQLFPRKCEDKPILANQFSVFVSRPNAEKYSTVLCAQSPETQREVESSGIGSWDWNLKG 242
           R+QLFP   E++P+L NQFSVFVSRPN EKYSTVLC QSPE  +E   SGIGSWDWNL G
Sbjct: 124 RFQLFPITSENEPVLENQFSVFVSRPNGEKYSTVLCRQSPEALKECPPSGIGSWDWNLNG 183

Query: 243 HSSTYHALYPRAWTIYDGEPDPELRIVCRQISPVIPHNYKESSYPVSVFTFTLHNSGKTD 302
           + STY ALYPRAWT+YDGEPDP L+IVCRQISP+IPHNYKESS+PV+VFTFTL NSGKT 
Sbjct: 184 NKSTYLALYPRAWTVYDGEPDPALKIVCRQISPIIPHNYKESSFPVAVFTFTLFNSGKTA 243

Query: 303 ADVSLLFTWANSVGGLSEYSGNHINSRTKKKDGVHTVLLHHKTASGFPPVTYAIAAQEGN 362
           AD++LLFTWANSVGG+S  SG H+NS+   KDGV  VLLHHKTA+G PPVTYAIAAQE +
Sbjct: 244 ADITLLFTWANSVGGVSGLSGQHLNSKFMMKDGVRGVLLHHKTANGRPPVTYAIAAQEMD 303

Query: 363 GVHVSDCPCFVISGNSQGISAKDMWLEIKEHGSFDRLKFADMSMPSEVGSSIGAAVSASV 422
           GVH+S+CPCF ISG++ GI+AKDMW EIKEHGSFDRL  A+ SMPSE+GSS+GAAV+AS+
Sbjct: 304 GVHISECPCFFISGDTPGITAKDMWNEIKEHGSFDRLNSAETSMPSELGSSVGAAVAASL 363

Query: 423 TVSSDSVRTVTFSLSWDCPEVNFCRGKTYHRRYTKFYGNLGDAAADIARDAILEHHHWES 482
           T+ SDS +TVTFSL+WDCPE+NF + +TY+RRYTKFYG  GDAAA IA DAIL+H HWES
Sbjct: 364 TIPSDSEQTVTFSLAWDCPEINFSKERTYYRRYTKFYGTRGDAAAKIAHDAILDHGHWES 423

Query: 483 QIDAWQRPVLEDKRFPKWYPVTLFNELYYLNAGGTIWTDGSLPIQSLVSIGERGFCLDQC 542
           QI+AWQ+PVLEDKRFP+WYP+TLFNELYYLN+GGT+WTDGS P+ S  SI ER F LD+ 
Sbjct: 424 QIEAWQKPVLEDKRFPEWYPITLFNELYYLNSGGTVWTDGSPPVHSFTSIIERKFSLDRS 483

Query: 543 RSG-DSVIYESHENDTANDILGRMTSRLDELRDSVASNSAFGVNLLQKGEENVGQFLYLE 602
           RS   + +  SH NDTA DIL RMTS L+++   V SNSAFG NLLQ+GEEN+GQFLYLE
Sbjct: 484 RSDLKNTVSISHHNDTAVDILERMTSVLEQVHTPVTSNSAFGPNLLQQGEENIGQFLYLE 543

Query: 603 GVEYTMWNTYDVHFYSSFAIIMLFPKLELSIQRDFAAAVMMHDPSKMQLLDNGKWEARNV 662
           GVEY MWNT DVHFYSSFA+IMLFPKLELSIQRDFAA+VMMHDPSKM+LL NGKW +R V
Sbjct: 544 GVEYFMWNTNDVHFYSSFALIMLFPKLELSIQRDFAASVMMHDPSKMKLLCNGKWVSRKV 603

Query: 663 LGAVPHDIGVNDPWFEVNGYNLYNTDRWKDLNPKFVLQIYRDVVATGDMKFAKAVWPPVY 722
           LGAVPHD+G  DPWFEVNGYNLYNTDRWKDLNPKFVLQ+YRDVVATGD  FAKAVWP VY
Sbjct: 604 LGAVPHDVGNYDPWFEVNGYNLYNTDRWKDLNPKFVLQVYRDVVATGDKNFAKAVWPSVY 663

Query: 723 LAIAYMDQFDRDGDGMIENDGFPDQTYDTWSVSGVSAYSGGLWVAALQAASALARVADEK 782
           +A+AYM+QFD+DGDGMIEN+GFPDQTYDTWSVSGVSAYSGGLWVAALQAASALARV  +K
Sbjct: 664 VALAYMNQFDKDGDGMIENEGFPDQTYDTWSVSGVSAYSGGLWVAALQAASALARVVGDK 723

Query: 783 DAEHYFWFKFQKAKRAYEKLWNGSYFNYDSSGGSSSSSIQADQLAGQWY 831
            +E YFWFKFQKAK  Y+KLWNGSYFNYD S GSSSSSIQADQLAGQWY
Sbjct: 724 GSEDYFWFKFQKAKGVYQKLWNGSYFNYDDSDGSSSSSIQADQLAGQWY 762

BLAST of Csa5G011660 vs. TrEMBL
Match: A0A067LD96_JATCU (Non-lysosomal glucosylceramidase OS=Jatropha curcas GN=JCGZ_00267 PE=3 SV=1)

HSP 1 Score: 1179.1 bits (3049), Expect = 0.0e+00
Identity = 568/777 (73.10%), Postives = 650/777 (83.66%), Query Frame = 1

Query: 55  MSEGKKLGNGLVEQDEDINKSLTEKVKWCIQSFFMTGIDPGQTPSLTWQRKLNREGTSLW 114
           M EG+ L NG  ++D + + SL +KV            DPG+  SLTWQRKLN E  +L 
Sbjct: 1   MFEGELLENGFNQEDREPSDSLVDKV------------DPGKPASLTWQRKLNAEEIALS 60

Query: 115 QFRLHLKEIIHLAPVGYRLFRYIREESVKGRGAMINPFIRRRITDTHAIPLGGLGSGSIG 174
           QF L  +E   LAPVG RL++++REE+ KGRG +I+PF +R +T T  +PLG +GSGSIG
Sbjct: 61  QFNLRFQEKFQLAPVGIRLWQFLREEAAKGRGGIIDPFAKRFLTSTQGVPLGAIGSGSIG 120

Query: 175 RSYRGEFQRWQLFPRKCEDKPILANQFSVFVSRPNAEKYSTVLCAQSPETQREVESSGIG 234
           RSY+GEFQRWQLFPR CE+KP+LANQFSVFVSR   EKYS+VLC + PE+ +E   SGI 
Sbjct: 121 RSYKGEFQRWQLFPRICEEKPVLANQFSVFVSRSTGEKYSSVLCPRKPESVKEPAVSGIE 180

Query: 235 SWDWNLKGHSSTYHALYPRAWTIYDGEPDPELRIVCRQISPVIPHNYKESSYPVSVFTFT 294
           SWDWNL G +STYHALYPRAWTIYDGEPDPELRIVCRQISPVIPHNYKESS+PVSVFTFT
Sbjct: 181 SWDWNLMGDNSTYHALYPRAWTIYDGEPDPELRIVCRQISPVIPHNYKESSFPVSVFTFT 240

Query: 295 LHNSGKTDADVSLLFTWANSVGGLSEYSGNHINSRTKKKDGVHTVLLHHKTASGFPPVTY 354
           L+NSGK  ADVSLLFTWANSVGG SE+SG H NS T  +DGVH+VLLHHKTASG PPVT+
Sbjct: 241 LYNSGKAAADVSLLFTWANSVGGDSEFSGQHFNSTTMMEDGVHSVLLHHKTASGLPPVTF 300

Query: 355 AIAAQEGNGVHVSDCPCFVISGNSQGISAKDMWLEIKEHGSFDRLKFADMSMPSEVGSSI 414
           AIAAQE NGVHVS CP FVISG+SQGI+AKDMW EIKEHGSFD LK +  S+PSE+GSSI
Sbjct: 301 AIAAQEINGVHVSKCPRFVISGHSQGITAKDMWDEIKEHGSFDNLKSSGTSVPSELGSSI 360

Query: 415 GAAVSASVTVSSDSVRTVTFSLSWDCPEVNFCRGKTYHRRYTKFYGNLGDAAADIARDAI 474
           GAA++AS  V  D+VRTVTFSL+WDCPEV F  G+TY+RRYT+FYG  GDAAA IA DAI
Sbjct: 361 GAAIAASAIVPPDAVRTVTFSLAWDCPEVYFVGGRTYNRRYTRFYGTHGDAAAKIAHDAI 420

Query: 475 LEHHHWESQIDAWQRPVLEDKRFPKWYPVTLFNELYYLNAGGTIWTDGSLPIQSLVSIGE 534
           L++  WESQI+AWQ P+LEDKR P+WYP+TLFNELYYLN+GGTIWTDGS P  +LVS G 
Sbjct: 421 LDNGSWESQIEAWQGPILEDKRLPEWYPITLFNELYYLNSGGTIWTDGSPPFHNLVSTGG 480

Query: 535 RGFCLDQCRSG-DSVIYESHENDTANDILGRMTSRLDELRDSVASNSAFGVNLLQKGEEN 594
           R F LD    G  SVI  +H+ND + DILGR+TS+L+++   VASNSAFG NLLQ+GEEN
Sbjct: 481 RKFSLDSSGVGLKSVIGINHQNDISTDILGRVTSKLEQIHAPVASNSAFGTNLLQEGEEN 540

Query: 595 VGQFLYLEGVEYTMWNTYDVHFYSSFAIIMLFPKLELSIQRDFAAAVMMHDPSKMQLLDN 654
           +GQFLYLEG+EY MWNTYDVHFYSSFA++MLFPKLELS+QRDFAAAVMMHDPSKMQLL +
Sbjct: 541 IGQFLYLEGIEYHMWNTYDVHFYSSFALVMLFPKLELSVQRDFAAAVMMHDPSKMQLLHD 600

Query: 655 GKWEARNVLGAVPHDIGVNDPWFEVNGYNLYNTDRWKDLNPKFVLQIYRDVVATGDMKFA 714
           G+W  R VLGAVPHDIG++DPWFEVN YNLYNTDRWKDLNPKFVLQ+YRDVVATGD KFA
Sbjct: 601 GQWVRRKVLGAVPHDIGIHDPWFEVNAYNLYNTDRWKDLNPKFVLQVYRDVVATGDKKFA 660

Query: 715 KAVWPPVYLAIAYMDQFDRDGDGMIENDGFPDQTYDTWSVSGVSAYSGGLWVAALQAASA 774
           +AVWP VYLA+A+MDQFDRDGDGMIENDGFPDQTYDTWSVSG+SAYSGGLW AALQAASA
Sbjct: 661 EAVWPSVYLAMAFMDQFDRDGDGMIENDGFPDQTYDTWSVSGLSAYSGGLWAAALQAASA 720

Query: 775 LARVADEKDAEHYFWFKFQKAKRAYEKLWNGSYFNYDSSGGSSSSSIQADQLAGQWY 831
           LAR   +K +E YFW +F KAK  YEKLWNGSYFNYD+SG S+SSSIQADQLAGQWY
Sbjct: 721 LAREVGDKGSEDYFWARFLKAKVVYEKLWNGSYFNYDNSGSSNSSSIQADQLAGQWY 765

BLAST of Csa5G011660 vs. TAIR10
Match: AT5G49900.1 (AT5G49900.1 Beta-glucosidase, GBA2 type family protein)

HSP 1 Score: 1085.5 bits (2806), Expect = 0.0e+00
Identity = 513/743 (69.04%), Postives = 601/743 (80.89%), Query Frame = 1

Query: 90  TGIDPGQTPSLTWQRKLNREGTSLWQFRLHLKEIIHLAPVGYRLFRYIREESVKGRGAMI 149
           T +DP    SLTWQRK++ +  +  +F L +KEI  LAPVG RL+   REE+ KGR A I
Sbjct: 21  TKVDPAVPASLTWQRKIDSDVKAPREFNLSVKEIFQLAPVGIRLWFLCREEAAKGRLAFI 80

Query: 150 NPFIRRRITDTHAIPLGGLGSGSIGRSYRGEFQRWQLFPRKCEDKPILANQFSVFVSRPN 209
           +PF +  +T +H +PLGG+G+GSIGRS++GEFQRWQLFP KCED+P+LANQFS FVSR N
Sbjct: 81  DPFSKHSVTSSHGVPLGGIGAGSIGRSFKGEFQRWQLFPPKCEDEPVLANQFSAFVSRAN 140

Query: 210 AEKYSTVLCAQSPETQREVESSGIGSWDWNLKGHSSTYHALYPRAWTIYDGEPDPELRIV 269
            +KYS+VLC ++P+  ++   SGIGSWDWNLKG  STYHALYPR+WT+Y+GEPDPELRIV
Sbjct: 141 GKKYSSVLCPRNPKLDKQDSESGIGSWDWNLKGDKSTYHALYPRSWTMYEGEPDPELRIV 200

Query: 270 CRQISPVIPHNYKESSYPVSVFTFTLHNSGKTDADVSLLFTWANSVGGLSEYSGNHINSR 329
           CRQ+SP IPHNYKESS+PVSVFTFTLHN G T ADV+LLFTWANSVGG SE+SG H NS+
Sbjct: 201 CRQVSPFIPHNYKESSFPVSVFTFTLHNLGNTTADVTLLFTWANSVGGDSEFSGGHYNSK 260

Query: 330 TKKKDGVHTVLLHHKTASGFPPVTYAIAAQEGNGVHVSDCPCFVISGNSQGISAKDMWLE 389
               DGV  VLLHHKTA+G P ++YAI+AQ  +GV VS CP F++SG   GI+AKDMW  
Sbjct: 261 ITMNDGVQGVLLHHKTANGLPSLSYAISAQATDGVSVSACPFFIVSGKQDGITAKDMWQA 320

Query: 390 IKEHGSFDRLKFADMSMPSEVGSSIGAAVSASVTVSSDSVRTVTFSLSWDCPEVNFCRGK 449
           +KE+GSFD LK ++ SM S+ GSSIGAAV+ASVTV     R VTFSL+WDCPEV F  GK
Sbjct: 321 VKENGSFDHLKASEASMQSDHGSSIGAAVAASVTVLPGESRIVTFSLAWDCPEVQFPSGK 380

Query: 450 TYHRRYTKFYGNLGDAAADIARDAILEHHHWESQIDAWQRPVLEDKRFPKWYPVTLFNEL 509
            Y RRYTKFYGN GDAAA IA DAIL H  WES I+ WQRP+LEDKR P WYPVTLFNEL
Sbjct: 381 IYSRRYTKFYGNNGDAAAQIAHDAILGHSQWESWIEDWQRPILEDKRLPAWYPVTLFNEL 440

Query: 510 YYLNAGGTIWTDGSLPIQSLVSIGERGFCLDQCRSG-DSVIYESHENDTANDILGRMTSR 569
           YYLN+GGT+WTDGS P+ SL  + E+ F LD+ + G  + I   H+NDTA  +L +M S 
Sbjct: 441 YYLNSGGTLWTDGSSPVHSLAGVREKKFSLDKSQLGLKNDIDVPHQNDTAVSVLEKMAST 500

Query: 570 LDELRDSVASNSAFGVNLLQKGEENVGQFLYLEGVEYTMWNTYDVHFYSSFAIIMLFPKL 629
           L+EL  S  SNSAFG  LL++GEEN+G FLYLEG+EY MWNTYDVHFY+SFA++MLFPKL
Sbjct: 501 LEELHASTTSNSAFGTKLLEEGEENIGHFLYLEGIEYRMWNTYDVHFYASFALVMLFPKL 560

Query: 630 ELSIQRDFAAAVMMHDPSKMQLLDNGKWEARNVLGAVPHDIGVNDPWFEVNGYNLYNTDR 689
           ELSIQRDFAAAVM+HDP+K++ L  G+W  R VLGAVPHD+G+NDPWFEVNGY L+NTDR
Sbjct: 561 ELSIQRDFAAAVMLHDPTKVKTLSEGQWVQRKVLGAVPHDLGINDPWFEVNGYTLHNTDR 620

Query: 690 WKDLNPKFVLQIYRDVVATGDMKFAKAVWPPVYLAIAYMDQFDRDGDGMIENDGFPDQTY 749
           WKDLNPKFVLQ+YRDVVATGD KFA AVWP VY+A+AYM QFD+DGDGMIEN+GFPDQTY
Sbjct: 621 WKDLNPKFVLQVYRDVVATGDKKFASAVWPSVYVAMAYMAQFDKDGDGMIENEGFPDQTY 680

Query: 750 DTWSVSGVSAYSGGLWVAALQAASALARVADEKDAEHYFWFKFQKAKRAYE-KLWNGSYF 809
           DTWS SGVSAY GGLWVAALQAASALARV  +K+++ YFW KFQKAK  YE KLWNGSYF
Sbjct: 681 DTWSASGVSAYCGGLWVAALQAASALARVVGDKNSQDYFWSKFQKAKVVYEKKLWNGSYF 740

Query: 810 NYDSSGGSSSSSIQADQLAGQWY 831
           NYD+SG   SS+IQADQLAGQWY
Sbjct: 741 NYDNSGSQYSSTIQADQLAGQWY 763

BLAST of Csa5G011660 vs. TAIR10
Match: AT1G33700.1 (AT1G33700.1 Beta-glucosidase, GBA2 type family protein)

HSP 1 Score: 941.4 bits (2432), Expect = 3.7e-274
Identity = 458/745 (61.48%), Postives = 559/745 (75.03%), Query Frame = 1

Query: 98  PSLTWQRKLNREGTSLWQFRLHLKEIIHLAPVGYRLFRYIREESVKGRGAMINPFIRRRI 157
           P  +W+RKLN +  +  +F+L  ++ +HL P+GYRL+R+ ++E+ KGR ++ + F +  I
Sbjct: 18  PPFSWERKLNSQAKTPSEFKLSKRDHMHLFPLGYRLWRHTKDEAAKGRASIFDIFRKHHI 77

Query: 158 TDTHAIPLGGLGSGSIGRSYRGEFQRWQLFPRKCEDKPILANQFSVFVSRPNAEKYSTVL 217
           T  H +PLGG+GSGSIGRSY+GEFQ+++LFP+ CE+ PIL NQFS FVSRP   K+STVL
Sbjct: 78  TGDHGVPLGGIGSGSIGRSYKGEFQQFKLFPKICEEAPILTNQFSAFVSRPGGVKHSTVL 137

Query: 218 CAQSPET---------QREVESSGIGSWDWNLKGHSSTYHALYPRAWTIYDGEPDPELRI 277
           C   P+          Q +  + GI SWDWN+ G  STYHALYPR+WT+YDGEPDPELRI
Sbjct: 138 CPSKPQVVKDNGGYLCQGQAPNMGIESWDWNMTGEKSTYHALYPRSWTVYDGEPDPELRI 197

Query: 278 VCRQISPVIPHNYKESSYPVSVFTFTLHNSGKTDADVSLLFTWANSVGGLSEYSGNHINS 337
           V RQ+SP IPHNY+ESS PVSVF FT+ N+G   A V+LLFTW NSVGG S  +G H NS
Sbjct: 198 VSRQVSPFIPHNYEESSLPVSVFNFTVTNTGAEPAIVTLLFTWENSVGGASGLTGQHFNS 257

Query: 338 RTKKKDGVHTVLLHHKTASGFPPVTYAIAAQEGNGVHVSDCPCFVISGNSQG-ISAKDMW 397
             K KDGVH V L HKTA+G PPV+YAIAA+E   V VS CPCF++SG +   I+A DMW
Sbjct: 258 TMKAKDGVHAVALQHKTANGHPPVSYAIAAKETEDVRVSSCPCFIVSGTTPNQITAGDMW 317

Query: 398 LEIKEHGSFDRLKFADMSMPSEVGSSIGAAVSASVTVSSDSVRTVTFSLSWDCPEVNFCR 457
            EIK++ SFD+L  ++   PS+ G+SIGAA++A V V     RTVTFSLSWDCPE  F  
Sbjct: 318 DEIKKNASFDKLT-SNACSPSKPGTSIGAAIAAKVKVPPGCDRTVTFSLSWDCPEARFDE 377

Query: 458 GKTYHRRYTKFYGNLGDAAADIARDAILEHHHWESQIDAWQRPVLEDKRFPKWYPVTLFN 517
            KTYHRRYT+FYG+LG+AA  +A DA+L    WE+QI+ WQ PVL D   P+WY VTLFN
Sbjct: 378 -KTYHRRYTRFYGSLGNAAVAMAHDALLNFPEWETQIEEWQAPVLADTTLPEWYRVTLFN 437

Query: 518 ELYYLNAGGTIWTDGSLPIQSLVSIGERGFCLDQCRSGDSVI--YESHENDTANDILGRM 577
           ELYY N+GGT+WTDG  P QSL SIG R   L     G S I   +  +N+ A DILGR+
Sbjct: 438 ELYYFNSGGTMWTDGLPPKQSLDSIGRRKISL-----GLSTIDKNDQDQNNVALDILGRI 497

Query: 578 TSRLDELRDSVASNSAFGVNLLQKGEENVGQFLYLEGVEYTMWNTYDVHFYSSFAIIMLF 637
            +   ++   + SN+A G  ++Q   EN+GQFLYLEGV+Y M+NTYDVHFYSSFA++MLF
Sbjct: 498 DAVCSQIHAPLTSNAALGNTMIQNTTENIGQFLYLEGVQYLMYNTYDVHFYSSFALLMLF 557

Query: 638 PKLELSIQRDFAAAVMMHDPSKMQLLDNGKWEARNVLGAVPHDIGVNDPWFEVNGYNLYN 697
           PK+ELSIQRDFAAAV+MHD SK Q++ +G++  R VLGAVPHDIG+NDPWFE+N YNL+N
Sbjct: 558 PKIELSIQRDFAAAVLMHDSSKKQVMSSGEFVTRKVLGAVPHDIGLNDPWFELNAYNLFN 617

Query: 698 TDRWKDLNPKFVLQIYRDVVATGDMKFAKAVWPPVYLAIAYMDQFDRDGDGMIENDGFPD 757
           TDRWKDLN KFVLQ+YRDVVATGD+ FAKAVWP VY AIAY+DQFD+DGDGMIEN+GFPD
Sbjct: 618 TDRWKDLNSKFVLQVYRDVVATGDLNFAKAVWPSVYTAIAYLDQFDKDGDGMIENEGFPD 677

Query: 758 QTYDTWSVSGVSAYSGGLWVAALQAASALARVADEKDAEHYFWFKFQKAKRAYEKLWNGS 817
           QTYD WS SGVSAY GGLWVAALQA SALAR   +  A  YF  K++KA+  YEKLWNGS
Sbjct: 678 QTYDAWSCSGVSAYCGGLWVAALQAGSALAREIGDNGAAVYFNAKYEKARSVYEKLWNGS 737

Query: 818 YFNYDSSGGSSSSSIQADQLAGQWY 831
           YFNYD+S   SSSSI ADQ+AGQWY
Sbjct: 738 YFNYDNSRSGSSSSILADQMAGQWY 755

BLAST of Csa5G011660 vs. TAIR10
Match: AT4G10060.1 (AT4G10060.1 Beta-glucosidase, GBA2 type family protein)

HSP 1 Score: 926.4 bits (2393), Expect = 1.2e-269
Identity = 447/735 (60.82%), Postives = 552/735 (75.10%), Query Frame = 1

Query: 98  PSLTWQRKLNREGTSLWQFRLHLKEIIHLAPVGYRLFRYIREESVKGRGAMINPFIRRRI 157
           P +TWQRKLN +  +  +F++ +++++HL P+GYRL+RY +EE+ KGR +M + F +R +
Sbjct: 22  PQVTWQRKLNSKVKNPSEFKMSIRDVLHLFPLGYRLWRYTKEEAKKGRFSMYDIFKKRHV 81

Query: 158 TDTHAIPLGGLGSGSIGRSYRGEFQRWQLFPRKCEDKPILANQFSVFVSRPNAEKYSTVL 217
              H +PLGG+G GSIGRSY+GEFQ+++LFP+ CE+ PIL NQFSVFVSRP    YSTVL
Sbjct: 82  RGDHGVPLGGIGGGSIGRSYKGEFQQFKLFPKICEEAPILTNQFSVFVSRPGGLSYSTVL 141

Query: 218 CAQSPETQR-EVESSGIGSWDWNLKGHSSTYHALYPRAWTIYDGEPDPELRIVCRQISPV 277
           C   P++ + + E  GI SWDWN++G  STYHALYPR+WT+Y+ EPDPELRIV RQ+SP 
Sbjct: 142 CPTKPKSVKGKTEDLGIESWDWNMEGDKSTYHALYPRSWTVYN-EPDPELRIVSRQVSPF 201

Query: 278 IPHNYKESSYPVSVFTFTLHNSGKTDADVSLLFTWANSVGGLSEYSGNHINSRTKKKDGV 337
           IPHNYKESS PVSVF FT+ N GK +A V+LLFTW NSVGG S  +G H NS   ++DGV
Sbjct: 202 IPHNYKESSLPVSVFEFTMSNLGKEEATVTLLFTWENSVGGASGLTGEHFNSTIMERDGV 261

Query: 338 HTVLLHHKTASGFPPVTYAIAAQEGNGVHVSDCPCFVISGNS-QGISAKDMWLEIKEHGS 397
           H ++LHHKT +G PPVTYAIAAQE   VHVS+CPCF++SG+S + I+AK+MW EIK++ S
Sbjct: 262 HAIVLHHKTGNGHPPVTYAIAAQETEDVHVSECPCFLVSGHSPKEITAKEMWDEIKKNKS 321

Query: 398 FDRLKFADMSMPSEVGSSIGAAVSASVTVSSDSVRTVTFSLSWDCPEVNFCRGKTYHRRY 457
           FD L  ++   PS  G+SIGAA++A V V     RTVTFSLSWDCPEV F   KTYHRRY
Sbjct: 322 FDELN-SEPGSPSRPGTSIGAAIAAKVKVPPGCDRTVTFSLSWDCPEVRF-NEKTYHRRY 381

Query: 458 TKFYGNLGDAAADIARDAILEHHHWESQIDAWQRPVLEDKRFPKWYPVTLFNELYYLNAG 517
           TKFYGNLGDAA  +ARDA+L +  WESQI+AWQ P+L D   P WY VTLFNELYY N+G
Sbjct: 382 TKFYGNLGDAAVKMARDALLNYVDWESQIEAWQSPILSDTTLPDWYRVTLFNELYYFNSG 441

Query: 518 GTIWTDGSLPIQSLVSIGERGFCLDQCRSGDSVIYESHENDTANDILGRMTSRLDELRDS 577
           GTIWTDG  P +S+    ER           S +  + +ND   D+  ++ +  +++   
Sbjct: 442 GTIWTDGLPPKESI----ER-----------SKVTNTEQNDIVIDLFQKINAVCEQIYSP 501

Query: 578 VASNSAFGVNLLQKGEENVGQFLYLEGVEYTMWNTYDVHFYSSFAIIMLFPKLELSIQRD 637
            +SNS          EEN+GQF+YLEG+EY M+NTYDVHFYSSFA++ LFPKL LSIQRD
Sbjct: 502 QSSNS----------EENIGQFIYLEGIEYLMYNTYDVHFYSSFALLSLFPKLALSIQRD 561

Query: 638 FAAAVMMHDPSKMQLLDNGKWEARNVLGAVPHDIGVNDPWFEVNGYNLYNTDRWKDLNPK 697
           FAA V++ DP+K +++ +G+W  R +LG+VPHDIG+NDPW E+N YN +NTDRWKDLN K
Sbjct: 562 FAATVLIQDPTKKKIMSSGEWVTRKLLGSVPHDIGLNDPWLELNEYNFFNTDRWKDLNAK 621

Query: 698 FVLQIYRDVVATGDMKFAKAVWPPVYLAIAYMDQFDRDGDGMIENDGFPDQTYDTWSVSG 757
           FVLQ+YRDVVAT D  FAKAVWP VY A+AY+DQFD+D DGMIEN+GFPDQTYD WSV+G
Sbjct: 622 FVLQVYRDVVATNDQSFAKAVWPSVYTAVAYLDQFDKDEDGMIENEGFPDQTYDAWSVTG 681

Query: 758 VSAYSGGLWVAALQAASALARVADEKDAEHYFWFKFQKAKRAYEKLWNGSYFNYDSSGGS 817
           VSAY GGLWVAALQAASA A +  E     YF  K++KAK  YEKLWNGSYFNYD SG  
Sbjct: 682 VSAYCGGLWVAALQAASAFASIVGENAVAIYFNAKYEKAKIVYEKLWNGSYFNYDDSGSG 728

Query: 818 SSSSIQADQLAGQWY 831
           SSSSI ADQLAGQWY
Sbjct: 742 SSSSILADQLAGQWY 728

BLAST of Csa5G011660 vs. TAIR10
Match: AT3G24180.1 (AT3G24180.1 Beta-glucosidase, GBA2 type family protein)

HSP 1 Score: 758.8 bits (1958), Expect = 3.4e-219
Identity = 390/747 (52.21%), Postives = 493/747 (66.00%), Query Frame = 1

Query: 93  DPGQTPSLTWQRKLNREGTSLWQFRLHLKEIIHLAPVGYRLFRYIREESVKGRGAMINPF 152
           D    P   W+R+LN     L +F +  +E I +  +G RL+ Y+REE+  GR A I+PF
Sbjct: 31  DSAAPPPHAWRRRLNCHANILKEFTITFREAIKMVRLGIRLWSYVREEASHGRKAPIDPF 90

Query: 153 IRRRI--TDTHAIPLGGLGSGSIGRSYRGEFQRWQLFPRKCEDKPILANQFSVFVSRPNA 212
            +     + +  +PLGG+GSGSI R +RGEF++WQ+ P  C+  P+++NQFS+F+SR   
Sbjct: 91  TKENCKPSASQGVPLGGMGSGSISRGFRGEFKQWQITPGTCDPSPMMSNQFSIFISRDGG 150

Query: 213 -EKYSTVLCAQSPETQREVESSGIGSWDWNLKGHSSTYHALYPRAWTIYDGEPDPELRIV 272
            +KY++VL      +  +    G+ SW WNL G  STYHAL+PRAWTIYDGEPDPEL+I 
Sbjct: 151 HKKYASVLSPGQHGSLGKSRDKGLSSWGWNLNGQHSTYHALFPRAWTIYDGEPDPELKIS 210

Query: 273 CRQISPVIPHNYKESSYPVSVFTFTLHNSGKTDADVSLLFTWANSVGGLSEYSGNHINSR 332
           CRQISP IP+NY++SS P +VF +TL N+GK  A VSLLFTWANS+GG S  SG H+N  
Sbjct: 211 CRQISPFIPNNYRDSSLPAAVFVYTLVNTGKERAKVSLLFTWANSMGGTSHMSGGHVNEP 270

Query: 333 TKKKDGVHTVLLHHKTASGFPPVTYAIAAQEGNGVHVSDCPCFVISGNSQGISAKDMWLE 392
              +DGV  VLLHHKT  G PPVT+AIAA E   V+V+  PCF +S +S   +AKDMW  
Sbjct: 271 FIGEDGVSGVLLHHKTGKGNPPVTFAIAASETQNVNVTVLPCFGLSEDSS-FTAKDMWDT 330

Query: 393 IKEHGSFDRLKF-ADMSMPSEVGSSIGAAVSASVTVSSDSVRTVTFSLSWDCPEVNFCRG 452
           +++ G FD+  F +  S PS  G +I AAVSAS  V +    TV+F+LSW  P+V F +G
Sbjct: 331 MEQDGKFDQENFNSGPSTPSLAGDTICAAVSASAWVEAHGKCTVSFALSWSSPKVKFSKG 390

Query: 453 KTYHRRYTKFYGNLGDAAADIARDAILEHHHWESQIDAWQRPVLEDKRFPKWYPVTLFNE 512
            TY RRYTKFYG    AA D+  DA+  +  WE  I+AWQ P+L D+R P+WY  TLFNE
Sbjct: 391 STYDRRYTKFYGTSPRAALDLVHDALTNYKRWEEDIEAWQNPILRDERLPEWYKFTLFNE 450

Query: 513 LYYLNAGGTIWTDGSLPIQSLVSIGERGFCLDQCRSGDSVIYESHENDTANDILGRMTSR 572
           LY+L AGGT+W D S    SL + G          + D  +     ND  ND LG   S 
Sbjct: 451 LYFLVAGGTVWIDSS----SLNANGNSQHQQSGLGNSDGKVGGLDINDQRND-LGNGNSV 510

Query: 573 LDELRDSVAS----NSAFGVNLLQKGEENVGQFLYLEGVEYTMWNTYDVHFYSSFAIIML 632
             +  D V++    N  F         ++VG+FLYLEGVEY MW TYDVHFY+S+A++ML
Sbjct: 511 GVKSNDEVSAIHNRNGLFVDTPHVDDGDDVGRFLYLEGVEYVMWCTYDVHFYASYALLML 570

Query: 633 FPKLELSIQRDFAAAVMMHDPSKMQLLDNGKWEARNVLGAVPHDIGVNDPWFEVNGYNLY 692
           FPK+EL+IQRDFA AV+  D  K++ L  G    R V GAVPHD+G++DPW E+N YN++
Sbjct: 571 FPKIELNIQRDFAKAVLSEDGRKVKFLAEGNVGIRKVRGAVPHDLGMHDPWNEMNAYNIH 630

Query: 693 NTDRWKDLNPKFVLQIYRDVVATGDMKFAKAVWPPVYLAIAYMDQFDRDGDGMIENDGFP 752
           +T RWKDLNPKFVLQ+YRD  ATGD +F   VWP V  A+ YM+QFDRD D +IENDGFP
Sbjct: 631 DTSRWKDLNPKFVLQVYRDFAATGDYQFGIDVWPAVRAAMEYMEQFDRDNDDLIENDGFP 690

Query: 753 DQTYDTWSVSGVSAYSGGLWVAALQAASALARVADEKDAEHYFWFKFQKAKRAYE-KLWN 812
           DQTYDTW+V GVSAY G LW+AALQAA+A+A    +K        KF  AK A E KLWN
Sbjct: 691 DQTYDTWTVHGVSAYCGCLWLAALQAAAAMALQIGDKFFAELCKNKFLNAKAALETKLWN 750

Query: 813 GSYFNYDSSGGSSSSSIQADQLAGQWY 831
           GSYFNYDS   S+S SIQ DQLAGQWY
Sbjct: 751 GSYFNYDSGSSSNSKSIQTDQLAGQWY 771

BLAST of Csa5G011660 vs. NCBI nr
Match: gi|700194399|gb|KGN49576.1| (hypothetical protein Csa_5G011660 [Cucumis sativus])

HSP 1 Score: 1719.5 bits (4452), Expect = 0.0e+00
Identity = 831/831 (100.00%), Postives = 831/831 (100.00%), Query Frame = 1

Query: 1   MENFPFPPLELSLRLSLLLLLLQFHFPFSSSSLSCFMDITSLITVSFFPHVDRNMSEGKK 60
           MENFPFPPLELSLRLSLLLLLLQFHFPFSSSSLSCFMDITSLITVSFFPHVDRNMSEGKK
Sbjct: 1   MENFPFPPLELSLRLSLLLLLLQFHFPFSSSSLSCFMDITSLITVSFFPHVDRNMSEGKK 60

Query: 61  LGNGLVEQDEDINKSLTEKVKWCIQSFFMTGIDPGQTPSLTWQRKLNREGTSLWQFRLHL 120
           LGNGLVEQDEDINKSLTEKVKWCIQSFFMTGIDPGQTPSLTWQRKLNREGTSLWQFRLHL
Sbjct: 61  LGNGLVEQDEDINKSLTEKVKWCIQSFFMTGIDPGQTPSLTWQRKLNREGTSLWQFRLHL 120

Query: 121 KEIIHLAPVGYRLFRYIREESVKGRGAMINPFIRRRITDTHAIPLGGLGSGSIGRSYRGE 180
           KEIIHLAPVGYRLFRYIREESVKGRGAMINPFIRRRITDTHAIPLGGLGSGSIGRSYRGE
Sbjct: 121 KEIIHLAPVGYRLFRYIREESVKGRGAMINPFIRRRITDTHAIPLGGLGSGSIGRSYRGE 180

Query: 181 FQRWQLFPRKCEDKPILANQFSVFVSRPNAEKYSTVLCAQSPETQREVESSGIGSWDWNL 240
           FQRWQLFPRKCEDKPILANQFSVFVSRPNAEKYSTVLCAQSPETQREVESSGIGSWDWNL
Sbjct: 181 FQRWQLFPRKCEDKPILANQFSVFVSRPNAEKYSTVLCAQSPETQREVESSGIGSWDWNL 240

Query: 241 KGHSSTYHALYPRAWTIYDGEPDPELRIVCRQISPVIPHNYKESSYPVSVFTFTLHNSGK 300
           KGHSSTYHALYPRAWTIYDGEPDPELRIVCRQISPVIPHNYKESSYPVSVFTFTLHNSGK
Sbjct: 241 KGHSSTYHALYPRAWTIYDGEPDPELRIVCRQISPVIPHNYKESSYPVSVFTFTLHNSGK 300

Query: 301 TDADVSLLFTWANSVGGLSEYSGNHINSRTKKKDGVHTVLLHHKTASGFPPVTYAIAAQE 360
           TDADVSLLFTWANSVGGLSEYSGNHINSRTKKKDGVHTVLLHHKTASGFPPVTYAIAAQE
Sbjct: 301 TDADVSLLFTWANSVGGLSEYSGNHINSRTKKKDGVHTVLLHHKTASGFPPVTYAIAAQE 360

Query: 361 GNGVHVSDCPCFVISGNSQGISAKDMWLEIKEHGSFDRLKFADMSMPSEVGSSIGAAVSA 420
           GNGVHVSDCPCFVISGNSQGISAKDMWLEIKEHGSFDRLKFADMSMPSEVGSSIGAAVSA
Sbjct: 361 GNGVHVSDCPCFVISGNSQGISAKDMWLEIKEHGSFDRLKFADMSMPSEVGSSIGAAVSA 420

Query: 421 SVTVSSDSVRTVTFSLSWDCPEVNFCRGKTYHRRYTKFYGNLGDAAADIARDAILEHHHW 480
           SVTVSSDSVRTVTFSLSWDCPEVNFCRGKTYHRRYTKFYGNLGDAAADIARDAILEHHHW
Sbjct: 421 SVTVSSDSVRTVTFSLSWDCPEVNFCRGKTYHRRYTKFYGNLGDAAADIARDAILEHHHW 480

Query: 481 ESQIDAWQRPVLEDKRFPKWYPVTLFNELYYLNAGGTIWTDGSLPIQSLVSIGERGFCLD 540
           ESQIDAWQRPVLEDKRFPKWYPVTLFNELYYLNAGGTIWTDGSLPIQSLVSIGERGFCLD
Sbjct: 481 ESQIDAWQRPVLEDKRFPKWYPVTLFNELYYLNAGGTIWTDGSLPIQSLVSIGERGFCLD 540

Query: 541 QCRSGDSVIYESHENDTANDILGRMTSRLDELRDSVASNSAFGVNLLQKGEENVGQFLYL 600
           QCRSGDSVIYESHENDTANDILGRMTSRLDELRDSVASNSAFGVNLLQKGEENVGQFLYL
Sbjct: 541 QCRSGDSVIYESHENDTANDILGRMTSRLDELRDSVASNSAFGVNLLQKGEENVGQFLYL 600

Query: 601 EGVEYTMWNTYDVHFYSSFAIIMLFPKLELSIQRDFAAAVMMHDPSKMQLLDNGKWEARN 660
           EGVEYTMWNTYDVHFYSSFAIIMLFPKLELSIQRDFAAAVMMHDPSKMQLLDNGKWEARN
Sbjct: 601 EGVEYTMWNTYDVHFYSSFAIIMLFPKLELSIQRDFAAAVMMHDPSKMQLLDNGKWEARN 660

Query: 661 VLGAVPHDIGVNDPWFEVNGYNLYNTDRWKDLNPKFVLQIYRDVVATGDMKFAKAVWPPV 720
           VLGAVPHDIGVNDPWFEVNGYNLYNTDRWKDLNPKFVLQIYRDVVATGDMKFAKAVWPPV
Sbjct: 661 VLGAVPHDIGVNDPWFEVNGYNLYNTDRWKDLNPKFVLQIYRDVVATGDMKFAKAVWPPV 720

Query: 721 YLAIAYMDQFDRDGDGMIENDGFPDQTYDTWSVSGVSAYSGGLWVAALQAASALARVADE 780
           YLAIAYMDQFDRDGDGMIENDGFPDQTYDTWSVSGVSAYSGGLWVAALQAASALARVADE
Sbjct: 721 YLAIAYMDQFDRDGDGMIENDGFPDQTYDTWSVSGVSAYSGGLWVAALQAASALARVADE 780

Query: 781 KDAEHYFWFKFQKAKRAYEKLWNGSYFNYDSSGGSSSSSIQADQLAGQWYI 832
           KDAEHYFWFKFQKAKRAYEKLWNGSYFNYDSSGGSSSSSIQADQLAGQWYI
Sbjct: 781 KDAEHYFWFKFQKAKRAYEKLWNGSYFNYDSSGGSSSSSIQADQLAGQWYI 831

BLAST of Csa5G011660 vs. NCBI nr
Match: gi|449463809|ref|XP_004149624.1| (PREDICTED: non-lysosomal glucosylceramidase [Cucumis sativus])

HSP 1 Score: 1580.5 bits (4091), Expect = 0.0e+00
Identity = 766/776 (98.71%), Postives = 766/776 (98.71%), Query Frame = 1

Query: 55  MSEGKKLGNGLVEQDEDINKSLTEKVKWCIQSFFMTGIDPGQTPSLTWQRKLNREGTSLW 114
           MSEGKKLGNGLVEQDEDINKSLTEK          TGIDPGQTPSLTWQRKLNREGTSLW
Sbjct: 1   MSEGKKLGNGLVEQDEDINKSLTEK----------TGIDPGQTPSLTWQRKLNREGTSLW 60

Query: 115 QFRLHLKEIIHLAPVGYRLFRYIREESVKGRGAMINPFIRRRITDTHAIPLGGLGSGSIG 174
           QFRLHLKEIIHLAPVGYRLFRYIREESVKGRGAMINPFIRRRITDTHAIPLGGLGSGSIG
Sbjct: 61  QFRLHLKEIIHLAPVGYRLFRYIREESVKGRGAMINPFIRRRITDTHAIPLGGLGSGSIG 120

Query: 175 RSYRGEFQRWQLFPRKCEDKPILANQFSVFVSRPNAEKYSTVLCAQSPETQREVESSGIG 234
           RSYRGEFQRWQLFPRKCEDKPILANQFSVFVSRPNAEKYSTVLCAQSPETQREVESSGIG
Sbjct: 121 RSYRGEFQRWQLFPRKCEDKPILANQFSVFVSRPNAEKYSTVLCAQSPETQREVESSGIG 180

Query: 235 SWDWNLKGHSSTYHALYPRAWTIYDGEPDPELRIVCRQISPVIPHNYKESSYPVSVFTFT 294
           SWDWNLKGHSSTYHALYPRAWTIYDGEPDPELRIVCRQISPVIPHNYKESSYPVSVFTFT
Sbjct: 181 SWDWNLKGHSSTYHALYPRAWTIYDGEPDPELRIVCRQISPVIPHNYKESSYPVSVFTFT 240

Query: 295 LHNSGKTDADVSLLFTWANSVGGLSEYSGNHINSRTKKKDGVHTVLLHHKTASGFPPVTY 354
           LHNSGKTDADVSLLFTWANSVGGLSEYSGNHINSRTKKKDGVHTVLLHHKTASGFPPVTY
Sbjct: 241 LHNSGKTDADVSLLFTWANSVGGLSEYSGNHINSRTKKKDGVHTVLLHHKTASGFPPVTY 300

Query: 355 AIAAQEGNGVHVSDCPCFVISGNSQGISAKDMWLEIKEHGSFDRLKFADMSMPSEVGSSI 414
           AIAAQEGNGVHVSDCPCFVISGNSQGISAKDMWLEIKEHGSFDRLKFADMSMPSEVGSSI
Sbjct: 301 AIAAQEGNGVHVSDCPCFVISGNSQGISAKDMWLEIKEHGSFDRLKFADMSMPSEVGSSI 360

Query: 415 GAAVSASVTVSSDSVRTVTFSLSWDCPEVNFCRGKTYHRRYTKFYGNLGDAAADIARDAI 474
           GAAVSASVTVSSDSVRTVTFSLSWDCPEVNFCRGKTYHRRYTKFYGNLGDAAADIARDAI
Sbjct: 361 GAAVSASVTVSSDSVRTVTFSLSWDCPEVNFCRGKTYHRRYTKFYGNLGDAAADIARDAI 420

Query: 475 LEHHHWESQIDAWQRPVLEDKRFPKWYPVTLFNELYYLNAGGTIWTDGSLPIQSLVSIGE 534
           LEHHHWESQIDAWQRPVLEDKRFPKWYPVTLFNELYYLNAGGTIWTDGSLPIQSLVSIGE
Sbjct: 421 LEHHHWESQIDAWQRPVLEDKRFPKWYPVTLFNELYYLNAGGTIWTDGSLPIQSLVSIGE 480

Query: 535 RGFCLDQCRSGDSVIYESHENDTANDILGRMTSRLDELRDSVASNSAFGVNLLQKGEENV 594
           RGFCLDQCRSGDSVIYESHENDTANDILGRMTSRLDELRDSVASNSAFGVNLLQKGEENV
Sbjct: 481 RGFCLDQCRSGDSVIYESHENDTANDILGRMTSRLDELRDSVASNSAFGVNLLQKGEENV 540

Query: 595 GQFLYLEGVEYTMWNTYDVHFYSSFAIIMLFPKLELSIQRDFAAAVMMHDPSKMQLLDNG 654
           GQFLYLEGVEYTMWNTYDVHFYSSFAIIMLFPKLELSIQRDFAAAVMMHDPSKMQLLDNG
Sbjct: 541 GQFLYLEGVEYTMWNTYDVHFYSSFAIIMLFPKLELSIQRDFAAAVMMHDPSKMQLLDNG 600

Query: 655 KWEARNVLGAVPHDIGVNDPWFEVNGYNLYNTDRWKDLNPKFVLQIYRDVVATGDMKFAK 714
           KWEARNVLGAVPHDIGVNDPWFEVNGYNLYNTDRWKDLNPKFVLQIYRDVVATGDMKFAK
Sbjct: 601 KWEARNVLGAVPHDIGVNDPWFEVNGYNLYNTDRWKDLNPKFVLQIYRDVVATGDMKFAK 660

Query: 715 AVWPPVYLAIAYMDQFDRDGDGMIENDGFPDQTYDTWSVSGVSAYSGGLWVAALQAASAL 774
           AVWPPVYLAIAYMDQFDRDGDGMIENDGFPDQTYDTWSVSGVSAYSGGLWVAALQAASAL
Sbjct: 661 AVWPPVYLAIAYMDQFDRDGDGMIENDGFPDQTYDTWSVSGVSAYSGGLWVAALQAASAL 720

Query: 775 ARVADEKDAEHYFWFKFQKAKRAYEKLWNGSYFNYDSSGGSSSSSIQADQLAGQWY 831
           ARVADEKDAEHYFWFKFQKAKRAYEKLWNGSYFNYDSSGGSSSSSIQADQLAGQWY
Sbjct: 721 ARVADEKDAEHYFWFKFQKAKRAYEKLWNGSYFNYDSSGGSSSSSIQADQLAGQWY 766

BLAST of Csa5G011660 vs. NCBI nr
Match: gi|659129612|ref|XP_008464753.1| (PREDICTED: non-lysosomal glucosylceramidase-like isoform X1 [Cucumis melo])

HSP 1 Score: 1542.7 bits (3993), Expect = 0.0e+00
Identity = 745/777 (95.88%), Postives = 756/777 (97.30%), Query Frame = 1

Query: 55  MSEGKKLGNGLVEQDEDINKSLTEKVKWCIQSFFMTGIDPGQTPSLTWQRKLNREGTSLW 114
           MSEGKKL NG VEQDEDINKSLTEK          TG DPGQTPSLTWQRKLNREG SLW
Sbjct: 1   MSEGKKLENGFVEQDEDINKSLTEK----------TGTDPGQTPSLTWQRKLNREGPSLW 60

Query: 115 QFRLHLKEIIHLAPVGYRLFRYIREESVKGRGAMINPFIRRRITDTHAIPLGGLGSGSIG 174
           QFRLHLKEIIHLAPVG+RL RYIREESVKGRGAMINPFIRRRITDTHA+PLGGLGSGSIG
Sbjct: 61  QFRLHLKEIIHLAPVGFRLIRYIREESVKGRGAMINPFIRRRITDTHAVPLGGLGSGSIG 120

Query: 175 RSYRGEFQRWQLFPRKCEDKPILANQFSVFVSRPNAEKYSTVLCAQSPETQREVESSGIG 234
           RSYRGEFQRWQLFPRKCEDKPILANQFSVFVSRPN EKYSTVLCAQSPE QREVESSGIG
Sbjct: 121 RSYRGEFQRWQLFPRKCEDKPILANQFSVFVSRPNGEKYSTVLCAQSPENQREVESSGIG 180

Query: 235 SWDWNLKGHSSTYHALYPRAWTIYDGEPDPELRIVCRQISPVIPHNYKESSYPVSVFTFT 294
           SWDWNLKGHSSTYHALYPRAWTIY+GEPDPELRIVCRQISPVIPHNYKESSYPVSVFTFT
Sbjct: 181 SWDWNLKGHSSTYHALYPRAWTIYNGEPDPELRIVCRQISPVIPHNYKESSYPVSVFTFT 240

Query: 295 LHNSGKTDADVSLLFTWANSVGGLSEYSGNHINSRTKKKDGVHTVLLHHKTASGFPPVTY 354
           LHNSGKTDADV+LLFTWANSVGGLSEYSGNHINSRTKKKDGVHTVLLHHKTASG+PPVTY
Sbjct: 241 LHNSGKTDADVNLLFTWANSVGGLSEYSGNHINSRTKKKDGVHTVLLHHKTASGYPPVTY 300

Query: 355 AIAAQEGNGVHVSDCPCFVISGNSQGISAKDMWLEIKEHGSFDRLKFADMSMPSEVGSSI 414
           AIAAQEGNGVHVSDCPCFVISG+SQGISAKDMWLEIKEHGSFDRLKFADMSMPSEVGSSI
Sbjct: 301 AIAAQEGNGVHVSDCPCFVISGHSQGISAKDMWLEIKEHGSFDRLKFADMSMPSEVGSSI 360

Query: 415 GAAVSASVTVSSDSVRTVTFSLSWDCPEVNFCRGKTYHRRYTKFYGNLGDAAADIARDAI 474
           GAA++AS+TVSSDSVRTVTFSLSWDCPEVNFCRGKTYHRRYTKFYGNLGDAAADIARDAI
Sbjct: 361 GAAITASITVSSDSVRTVTFSLSWDCPEVNFCRGKTYHRRYTKFYGNLGDAAADIARDAI 420

Query: 475 LEHHHWESQIDAWQRPVLEDKRFPKWYPVTLFNELYYLNAGGTIWTDGSLPIQSLVSIGE 534
           LEHHHWESQIDAWQRPVLEDKRFPKWYPVTLFNELYYLNAGGTIWTDGSLPIQSLVSIGE
Sbjct: 421 LEHHHWESQIDAWQRPVLEDKRFPKWYPVTLFNELYYLNAGGTIWTDGSLPIQSLVSIGE 480

Query: 535 RGFCLDQCRSG-DSVIYESHENDTANDILGRMTSRLDELRDSVASNSAFGVNLLQKGEEN 594
           RGFCLDQCRSG  SVIYESHENDTANDILGRMTSRLDELRDSV SNSAFGVNLLQKGEEN
Sbjct: 481 RGFCLDQCRSGLKSVIYESHENDTANDILGRMTSRLDELRDSVESNSAFGVNLLQKGEEN 540

Query: 595 VGQFLYLEGVEYTMWNTYDVHFYSSFAIIMLFPKLELSIQRDFAAAVMMHDPSKMQLLDN 654
           VGQFLYLEGVEYTMWNTYDVHFYSSFAIIMLFPKLELSIQRDFAAAVMMHDPSKMQLLDN
Sbjct: 541 VGQFLYLEGVEYTMWNTYDVHFYSSFAIIMLFPKLELSIQRDFAAAVMMHDPSKMQLLDN 600

Query: 655 GKWEARNVLGAVPHDIGVNDPWFEVNGYNLYNTDRWKDLNPKFVLQIYRDVVATGDMKFA 714
           GKWEARNVLGAVPHDIGVNDPWFEVNGYNLYNTDRWKDLNPKFVLQIYRDVVATGDMKFA
Sbjct: 601 GKWEARNVLGAVPHDIGVNDPWFEVNGYNLYNTDRWKDLNPKFVLQIYRDVVATGDMKFA 660

Query: 715 KAVWPPVYLAIAYMDQFDRDGDGMIENDGFPDQTYDTWSVSGVSAYSGGLWVAALQAASA 774
           KAVWPPVYL+IAYMDQFDRDGDGMIENDGFPDQTYDTWSV+GVSAYSGGLWVAALQAASA
Sbjct: 661 KAVWPPVYLSIAYMDQFDRDGDGMIENDGFPDQTYDTWSVTGVSAYSGGLWVAALQAASA 720

Query: 775 LARVADEKDAEHYFWFKFQKAKRAYEKLWNGSYFNYDSSGGSSSSSIQADQLAGQWY 831
           LARV DEKDAEHYFWFKFQKAKRAYEKLWNGSYFNYDSSGGSSSSSIQADQLAGQWY
Sbjct: 721 LARVVDEKDAEHYFWFKFQKAKRAYEKLWNGSYFNYDSSGGSSSSSIQADQLAGQWY 767

BLAST of Csa5G011660 vs. NCBI nr
Match: gi|659129614|ref|XP_008464754.1| (PREDICTED: non-lysosomal glucosylceramidase-like isoform X2 [Cucumis melo])

HSP 1 Score: 1395.9 bits (3612), Expect = 0.0e+00
Identity = 668/684 (97.66%), Postives = 678/684 (99.12%), Query Frame = 1

Query: 148 MINPFIRRRITDTHAIPLGGLGSGSIGRSYRGEFQRWQLFPRKCEDKPILANQFSVFVSR 207
           MINPFIRRRITDTHA+PLGGLGSGSIGRSYRGEFQRWQLFPRKCEDKPILANQFSVFVSR
Sbjct: 1   MINPFIRRRITDTHAVPLGGLGSGSIGRSYRGEFQRWQLFPRKCEDKPILANQFSVFVSR 60

Query: 208 PNAEKYSTVLCAQSPETQREVESSGIGSWDWNLKGHSSTYHALYPRAWTIYDGEPDPELR 267
           PN EKYSTVLCAQSPE QREVESSGIGSWDWNLKGHSSTYHALYPRAWTIY+GEPDPELR
Sbjct: 61  PNGEKYSTVLCAQSPENQREVESSGIGSWDWNLKGHSSTYHALYPRAWTIYNGEPDPELR 120

Query: 268 IVCRQISPVIPHNYKESSYPVSVFTFTLHNSGKTDADVSLLFTWANSVGGLSEYSGNHIN 327
           IVCRQISPVIPHNYKESSYPVSVFTFTLHNSGKTDADV+LLFTWANSVGGLSEYSGNHIN
Sbjct: 121 IVCRQISPVIPHNYKESSYPVSVFTFTLHNSGKTDADVNLLFTWANSVGGLSEYSGNHIN 180

Query: 328 SRTKKKDGVHTVLLHHKTASGFPPVTYAIAAQEGNGVHVSDCPCFVISGNSQGISAKDMW 387
           SRTKKKDGVHTVLLHHKTASG+PPVTYAIAAQEGNGVHVSDCPCFVISG+SQGISAKDMW
Sbjct: 181 SRTKKKDGVHTVLLHHKTASGYPPVTYAIAAQEGNGVHVSDCPCFVISGHSQGISAKDMW 240

Query: 388 LEIKEHGSFDRLKFADMSMPSEVGSSIGAAVSASVTVSSDSVRTVTFSLSWDCPEVNFCR 447
           LEIKEHGSFDRLKFADMSMPSEVGSSIGAA++AS+TVSSDSVRTVTFSLSWDCPEVNFCR
Sbjct: 241 LEIKEHGSFDRLKFADMSMPSEVGSSIGAAITASITVSSDSVRTVTFSLSWDCPEVNFCR 300

Query: 448 GKTYHRRYTKFYGNLGDAAADIARDAILEHHHWESQIDAWQRPVLEDKRFPKWYPVTLFN 507
           GKTYHRRYTKFYGNLGDAAADIARDAILEHHHWESQIDAWQRPVLEDKRFPKWYPVTLFN
Sbjct: 301 GKTYHRRYTKFYGNLGDAAADIARDAILEHHHWESQIDAWQRPVLEDKRFPKWYPVTLFN 360

Query: 508 ELYYLNAGGTIWTDGSLPIQSLVSIGERGFCLDQCRSG-DSVIYESHENDTANDILGRMT 567
           ELYYLNAGGTIWTDGSLPIQSLVSIGERGFCLDQCRSG  SVIYESHENDTANDILGRMT
Sbjct: 361 ELYYLNAGGTIWTDGSLPIQSLVSIGERGFCLDQCRSGLKSVIYESHENDTANDILGRMT 420

Query: 568 SRLDELRDSVASNSAFGVNLLQKGEENVGQFLYLEGVEYTMWNTYDVHFYSSFAIIMLFP 627
           SRLDELRDSV SNSAFGVNLLQKGEENVGQFLYLEGVEYTMWNTYDVHFYSSFAIIMLFP
Sbjct: 421 SRLDELRDSVESNSAFGVNLLQKGEENVGQFLYLEGVEYTMWNTYDVHFYSSFAIIMLFP 480

Query: 628 KLELSIQRDFAAAVMMHDPSKMQLLDNGKWEARNVLGAVPHDIGVNDPWFEVNGYNLYNT 687
           KLELSIQRDFAAAVMMHDPSKMQLLDNGKWEARNVLGAVPHDIGVNDPWFEVNGYNLYNT
Sbjct: 481 KLELSIQRDFAAAVMMHDPSKMQLLDNGKWEARNVLGAVPHDIGVNDPWFEVNGYNLYNT 540

Query: 688 DRWKDLNPKFVLQIYRDVVATGDMKFAKAVWPPVYLAIAYMDQFDRDGDGMIENDGFPDQ 747
           DRWKDLNPKFVLQIYRDVVATGDMKFAKAVWPPVYL+IAYMDQFDRDGDGMIENDGFPDQ
Sbjct: 541 DRWKDLNPKFVLQIYRDVVATGDMKFAKAVWPPVYLSIAYMDQFDRDGDGMIENDGFPDQ 600

Query: 748 TYDTWSVSGVSAYSGGLWVAALQAASALARVADEKDAEHYFWFKFQKAKRAYEKLWNGSY 807
           TYDTWSV+GVSAYSGGLWVAALQAASALARV DEKDAEHYFWFKFQKAKRAYEKLWNGSY
Sbjct: 601 TYDTWSVTGVSAYSGGLWVAALQAASALARVVDEKDAEHYFWFKFQKAKRAYEKLWNGSY 660

Query: 808 FNYDSSGGSSSSSIQADQLAGQWY 831
           FNYDSSGGSSSSSIQADQLAGQWY
Sbjct: 661 FNYDSSGGSSSSSIQADQLAGQWY 684

BLAST of Csa5G011660 vs. NCBI nr
Match: gi|1000947258|ref|XP_015580670.1| (PREDICTED: non-lysosomal glucosylceramidase isoform X2 [Ricinus communis])

HSP 1 Score: 1198.3 bits (3099), Expect = 0.0e+00
Identity = 582/777 (74.90%), Postives = 649/777 (83.53%), Query Frame = 1

Query: 55  MSEGKKLGNGLVEQDEDINKSLTEKVKWCIQSFFMTGIDPGQTPSLTWQRKLNREGTSLW 114
           MSEG+   NG  ++D + + SL EKV            DPG   SLTWQRKLN E  +L 
Sbjct: 1   MSEGEIPANGC-QEDREPSDSLLEKV------------DPGSPASLTWQRKLNSEDIALS 60

Query: 115 QFRLHLKEIIHLAPVGYRLFRYIREESVKGRGAMINPFIRRRITDTHAIPLGGLGSGSIG 174
           QF L  +E   LAPVG RL+R IREE+ KGR  +INPF++R IT  H IPLGG+GSGSIG
Sbjct: 61  QFNLSFQEKFQLAPVGIRLWRLIREETAKGRAGIINPFLKRFITSCHGIPLGGIGSGSIG 120

Query: 175 RSYRGEFQRWQLFPRKCEDKPILANQFSVFVSRPNAEKYSTVLCAQSPETQREVESSGIG 234
           RSY+GEFQRWQLFPR CE+KP+LANQFSVFVSR + EKYS+VLC ++PE   E   SGIG
Sbjct: 121 RSYKGEFQRWQLFPRICEEKPVLANQFSVFVSRSSGEKYSSVLCPRNPEVLMEPAISGIG 180

Query: 235 SWDWNLKGHSSTYHALYPRAWTIYDGEPDPELRIVCRQISPVIPHNYKESSYPVSVFTFT 294
           SWDWNLKG +STYHALYPRAWTIYDGEPDPELRIVCRQISP+IPHNYKESSYPVSVFTFT
Sbjct: 181 SWDWNLKGDNSTYHALYPRAWTIYDGEPDPELRIVCRQISPIIPHNYKESSYPVSVFTFT 240

Query: 295 LHNSGKTDADVSLLFTWANSVGGLSEYSGNHINSRTKKKDGVHTVLLHHKTASGFPPVTY 354
           L+NSGKT ADVSLLFTW NSVGG SEYSG H NS T  +DGVH VLLHHKTA GFPPVT+
Sbjct: 241 LYNSGKTTADVSLLFTWTNSVGGNSEYSGQHFNSTTMMEDGVHAVLLHHKTAEGFPPVTF 300

Query: 355 AIAAQEGNGVHVSDCPCFVISGNSQGISAKDMWLEIKEHGSFDRLKFADMSMPSEVGSSI 414
           AIAAQE N VHVS CP FVISGN QGI+AKDMW E+KEHGSFD LK A  S PSE GSSI
Sbjct: 301 AIAAQETNDVHVSKCPRFVISGNCQGITAKDMWHEVKEHGSFDNLKSAGTSGPSEPGSSI 360

Query: 415 GAAVSASVTVSSDSVRTVTFSLSWDCPEVNFCRGKTYHRRYTKFYGNLGDAAADIARDAI 474
           GAA++ASVT+  D++R+VTFSLSWDCPEV F  G+TYHRRYTKFY   GDAAA IA DAI
Sbjct: 361 GAAIAASVTIPPDAIRSVTFSLSWDCPEVYFMGGRTYHRRYTKFYSTHGDAAARIAHDAI 420

Query: 475 LEHHHWESQIDAWQRPVLEDKRFPKWYPVTLFNELYYLNAGGTIWTDGSLPIQSLVSIGE 534
           LEH  WESQI AWQRP+LEDKR P+WYP+TLFNELYYLN+GGTIWTDGS P  +LVSI  
Sbjct: 421 LEHGLWESQIVAWQRPILEDKRLPEWYPITLFNELYYLNSGGTIWTDGSPPYHNLVSIRG 480

Query: 535 RGFCLDQCRSG-DSVIYESHENDTANDILGRMTSRLDELRDSVASNSAFGVNLLQKGEEN 594
             F LD   +G  S+I  +HENDTA +ILGRMTS L+++   VASNSAFG NLLQ+GEEN
Sbjct: 481 SKFSLDTSGAGLKSIIDVTHENDTAVNILGRMTSALEQIHAHVASNSAFGTNLLQEGEEN 540

Query: 595 VGQFLYLEGVEYTMWNTYDVHFYSSFAIIMLFPKLELSIQRDFAAAVMMHDPSKMQLLDN 654
           +GQFLYLEG+EY MWNTYDVHFYSSFA++MLFPKLELS+QRDFAAAVMMHDPSKMQLL +
Sbjct: 541 IGQFLYLEGIEYHMWNTYDVHFYSSFALVMLFPKLELSVQRDFAAAVMMHDPSKMQLLHD 600

Query: 655 GKWEARNVLGAVPHDIGVNDPWFEVNGYNLYNTDRWKDLNPKFVLQIYRDVVATGDMKFA 714
           G+W  R VLGAVPHDIG+NDPW+EVN Y+LYNTDRWKDLNPKFVLQ+YRDVVATGD KFA
Sbjct: 601 GQWVCRKVLGAVPHDIGINDPWYEVNAYSLYNTDRWKDLNPKFVLQVYRDVVATGDKKFA 660

Query: 715 KAVWPPVYLAIAYMDQFDRDGDGMIENDGFPDQTYDTWSVSGVSAYSGGLWVAALQAASA 774
           +AVWP VY+A+AYMDQFDRDGDGMIENDGFPDQTYDTWSVSGVSAYSGGLWVAALQAASA
Sbjct: 661 EAVWPSVYIAMAYMDQFDRDGDGMIENDGFPDQTYDTWSVSGVSAYSGGLWVAALQAASA 720

Query: 775 LARVADEKDAEHYFWFKFQKAKRAYEKLWNGSYFNYDSSGGSSSSSIQADQLAGQWY 831
           LAR   +K +E YFW +FQKAK  Y+KLWNGSYFNYD+SGG +SSSIQADQLAGQWY
Sbjct: 721 LAREVGDKGSEDYFWARFQKAKDVYDKLWNGSYFNYDNSGGRNSSSIQADQLAGQWY 764

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
GBA2_RAT5.2e-6835.14Non-lysosomal glucosylceramidase OS=Rattus norvegicus GN=Gba2 PE=2 SV=2[more]
GBA2_MOUSE3.4e-6735.01Non-lysosomal glucosylceramidase OS=Mus musculus GN=Gba2 PE=1 SV=2[more]
GBA2_HUMAN3.7e-6634.02Non-lysosomal glucosylceramidase OS=Homo sapiens GN=GBA2 PE=1 SV=2[more]
C3390_DROME1.2e-5630.35Non-lysosomal glucosylceramidase OS=Drosophila melanogaster GN=CG33090 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KPF7_CUCSA0.0e+00100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_5G011660 PE=4 SV=1[more]
B9SS77_RICCO0.0e+0074.90Non-lysosomal glucosylceramidase OS=Ricinus communis GN=RCOM_0618840 PE=3 SV=1[more]
M5XQ56_PRUPE0.0e+0073.23Non-lysosomal glucosylceramidase OS=Prunus persica GN=PRUPE_ppa000954mg PE=3 SV=... [more]
D7TGQ9_VITVI0.0e+0073.08Non-lysosomal glucosylceramidase OS=Vitis vinifera GN=VIT_12s0035g01220 PE=3 SV=... [more]
A0A067LD96_JATCU0.0e+0073.10Non-lysosomal glucosylceramidase OS=Jatropha curcas GN=JCGZ_00267 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G49900.10.0e+0069.04 Beta-glucosidase, GBA2 type family protein[more]
AT1G33700.13.7e-27461.48 Beta-glucosidase, GBA2 type family protein[more]
AT4G10060.11.2e-26960.82 Beta-glucosidase, GBA2 type family protein[more]
AT3G24180.13.4e-21952.21 Beta-glucosidase, GBA2 type family protein[more]
Match NameE-valueIdentityDescription
gi|700194399|gb|KGN49576.1|0.0e+00100.00hypothetical protein Csa_5G011660 [Cucumis sativus][more]
gi|449463809|ref|XP_004149624.1|0.0e+0098.71PREDICTED: non-lysosomal glucosylceramidase [Cucumis sativus][more]
gi|659129612|ref|XP_008464753.1|0.0e+0095.88PREDICTED: non-lysosomal glucosylceramidase-like isoform X1 [Cucumis melo][more]
gi|659129614|ref|XP_008464754.1|0.0e+0097.66PREDICTED: non-lysosomal glucosylceramidase-like isoform X2 [Cucumis melo][more]
gi|1000947258|ref|XP_015580670.1|0.0e+0074.90PREDICTED: non-lysosomal glucosylceramidase isoform X2 [Ricinus communis][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR006775GH116_catalytic
IPR0089286-hairpin_glycosidase_sf
IPR024462GH116_N
Vocabulary: Molecular Function
TermDefinition
GO:0004553hydrolase activity, hydrolyzing O-glycosyl compounds
GO:0003824catalytic activity
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
biological_process GO:0006680 glucosylceramide catabolic process
biological_process GO:0006629 lipid metabolic process
cellular_component GO:0005794 Golgi apparatus
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005575 cellular_component
cellular_component GO:0016020 membrane
molecular_function GO:0004348 glucosylceramidase activity
molecular_function GO:0003824 catalytic activity
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds
This gene is associated with the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
CU123538cucumber EST collection version 3.0transcribed_cluster
CU124332cucumber EST collection version 3.0transcribed_cluster
CU126637cucumber EST collection version 3.0transcribed_cluster
CU156865cucumber EST collection version 3.0transcribed_cluster

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csa5G011660.1Csa5G011660.1mRNA


The following transcribed_cluster feature(s) are associated with this gene:

Feature NameUnique NameType
CU126637CU126637transcribed_cluster
CU156865CU156865transcribed_cluster
CU123538CU123538transcribed_cluster
CU124332CU124332transcribed_cluster


Analysis Name: InterPro Annotations of cucumber (Chinese Long)
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006775Glycosyl-hydrolase family 116, catalytic regionPFAMPF04685DUF608coord: 595..830
score: 2.7
IPR008928Six-hairpin glycosidase-likeunknownSSF48208Six-hairpin glycosidasescoord: 605..824
score: 5.35
IPR024462Glycosyl-hydrolase family 116, N-terminalPFAMPF12215Glyco_hydr_116Ncoord: 162..480
score: 6.3E
NoneNo IPR availablePANTHERPTHR12654BILE ACID BETA-GLUCOSIDASE-RELATEDcoord: 561..830
score: 0.0coord: 53..520
score:
NoneNo IPR availablePANTHERPTHR12654:SF3NON-LYSOSOMAL GLUCOSYLCERAMIDASEcoord: 53..520
score: 0.0coord: 561..830
score: