CSPI06G05810.1 (mRNA) Wild cucumber (PI 183967) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CSPI06G05810.1
Type	mRNA
Organism	Cucumis sativus (Wild cucumber (PI 183967))
Description	EF hand calcium-binding family protein
Location	Chr6 : 5196446 .. 5197472 (-)
Sequence length	537

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AATTAAGAGCCGTAGGACTTTAAACTTCTCTTCTACTTACATAAACTGTTTCTTTTCACAATTTCTCTTATAGAAAAATTTCACTTCTCTTAGTTATGTTCAATCTTACCTCTTCCTTATTCCCTACTTTCTTCCATAGGAATATTTCCTCAAAACCCTTCTTCCAAAAATCCCTAAACAACCAAAAGTTACCATCTTTCACCACTTTTAACCACTTCCAGCTCCTCTTCAACCTCCTCGACTCCGATGGTGACGGCAAGATCTCGATGAAAGAGCTCAGTCAATTTCTCTATCGTCTAGGATATAAAAAGCTGAAAGCTACAATGGAAGCTGAAGAAATGGTGAAGGAAATGGATTCAGACCGTGATGGATTCATTGAAATGGATGAATTTTTAGAGTGAGTATGACTTACATATTAGTTTAAGCACTTTTTATAAAACGTGCTTCAACTTTTGATCTAACATAGACCATTTAAATACTACTTTGATTTCATTTTAATCTTTATACTTTCAAATTTGGCTTTTTTTTTTTTTTTGACTGTACTTGTATATTTTGGAAAATTTAGAGAGACGACTGGGTTAGGCGTAACGGATGGTAAACACATTCTTAGCTTTTAATTTTGATGACGAAACGTTTTAATATAGGGTTTTGCAGAAGGGGGAGGAAGAAGAAGAAATTCTTAGGGAAGCTTTTCTGATATTTGATGGTGATAAGAATGGATTGATTTGTGCTGAGAAATTAAAGAGAACATTGAGCAAATTTGGATGGAAAAAATGTGACCTCAAACAATGTAAAAAGATGATTGAAGGAGTTGATCATGATGGCGATGGCTTTGTGAATTTTCAAGACTTTCGGCTCATGATGACACAGAAAAGATAATCATATATATAATATAGAGATTATTATCTGTAATTTTATATTTGATTTATCTCTAAGAAATGTAATGTCGTCTTTAATTAATGCTTATGATTATGACTTGTAATAATATATCTTCTTTTTCTTTTCTTTTTTTAAAAATTGTAATGGG

mRNA sequence

ATGTTCAATCTTACCTCTTCCTTATTCCCTACTTTCTTCCATAGGAATATTTCCTCAAAACCCTTCTTCCAAAAATCCCTAAACAACCAAAAGTTACCATCTTTCACCACTTTTAACCACTTCCAGCTCCTCTTCAACCTCCTCGACTCCGATGGTGACGGCAAGATCTCGATGAAAGAGCTCAGTCAATTTCTCTATCGTCTAGGATATAAAAAGCTGAAAGCTACAATGGAAGCTGAAGAAATGGTGAAGGAAATGGATTCAGACCGTGATGGATTCATTGAAATGGATGAATTTTTAGAGGTTTTGCAGAAGGGGGAGGAAGAAGAAGAAATTCTTAGGGAAGCTTTTCTGATATTTGATGGTGATAAGAATGGATTGATTTGTGCTGAGAAATTAAAGAGAACATTGAGCAAATTTGGATGGAAAAAATGTGACCTCAAACAATGTAAAAAGATGATTGAAGGAGTTGATCATGATGGCGATGGCTTTGTGAATTTTCAAGACTTTCGGCTCATGATGACACAGAAAAGATAA

Coding sequence (CDS)

ATGTTCAATCTTACCTCTTCCTTATTCCCTACTTTCTTCCATAGGAATATTTCCTCAAAACCCTTCTTCCAAAAATCCCTAAACAACCAAAAGTTACCATCTTTCACCACTTTTAACCACTTCCAGCTCCTCTTCAACCTCCTCGACTCCGATGGTGACGGCAAGATCTCGATGAAAGAGCTCAGTCAATTTCTCTATCGTCTAGGATATAAAAAGCTGAAAGCTACAATGGAAGCTGAAGAAATGGTGAAGGAAATGGATTCAGACCGTGATGGATTCATTGAAATGGATGAATTTTTAGAGGTTTTGCAGAAGGGGGAGGAAGAAGAAGAAATTCTTAGGGAAGCTTTTCTGATATTTGATGGTGATAAGAATGGATTGATTTGTGCTGAGAAATTAAAGAGAACATTGAGCAAATTTGGATGGAAAAAATGTGACCTCAAACAATGTAAAAAGATGATTGAAGGAGTTGATCATGATGGCGATGGCTTTGTGAATTTTCAAGACTTTCGGCTCATGATGACACAGAAAAGATAA

Homology

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BLAST of CSPI06G05810.1 vs. Swiss-Prot
Match: CML25_ARATH (Probable calcium-binding protein CML25 OS=Arabidopsis thaliana GN=CML25 PE=2 SV=1)

HSP 1 Score: 107.8 bits (268), Expect = 1.2e-22
Identity = 59/161 (36.65%), Postives = 97/161 (60.25%), Query Frame = 1


Query: 18  SSKPFFQKSLNNQKLPSFTTFNHFQLLFNLLDSDGDGKISMKELSQFLYRLGYKKLKATM 77
           +  P+ QK+ + +     T     + +F   D +GDGKIS KEL   +  LG++  +   
Sbjct: 20  ADSPYLQKARSGK-----TEIRELEAVFKKFDVNGDGKISSKELGAIMTSLGHEVPEE-- 79

Query: 78  EAEEMVKEMDSDRDGFIEMDEFLEVLQKGEEEEEIL---REAFLIFDGDKNGLICAEKLK 137
           E E+ + E+D   DG+I  +EF+E+  KG ++ ++L   ++AF ++D D NG I AE+L 
Sbjct: 80  ELEKAITEIDRKGDGYINFEEFVELNTKGMDQNDVLENLKDAFSVYDIDGNGSISAEELH 139

Query: 138 RTLSKFGWKKCDLKQCKKMIEGVDHDGDGFVNFQDFRLMMT 176
             L   G  +C + +C+KMI GVD DGDG ++F++F++MMT
Sbjct: 140 EVLRSLG-DECSIAECRKMIGGVDKDGDGTIDFEEFKIMMT 172

[Close]

BLAST of CSPI06G05810.1 vs. Swiss-Prot
Match: CML27_ARATH (Probable calcium-binding protein CML27 OS=Arabidopsis thaliana GN=CML27 PE=1 SV=1)

HSP 1 Score: 101.7 bits (252), Expect = 8.7e-21
Identity = 56/132 (42.42%), Postives = 84/132 (63.64%), Query Frame = 1


Query: 44  LFNLLDSDGDGKISMKELSQFLYRLGYKKLKATMEAEEMVKEMDSDRDGFIEMDEFLEVL 103
           +F+  DS+GDGKIS+ EL      +G    +   E   +++E+D+DRDG+I +DEF  + 
Sbjct: 27  VFDQFDSNGDGKISVLELGGVFKAMGTSYTET--ELNRVLEEVDTDRDGYINLDEFSTLC 86

Query: 104 QKGEEEEEILREAFLIFDGDKNGLICAEKLKRTLSKFGWKKCDLKQCKKMIEGVDHDGDG 163
           +      EI R+AF ++D DKNGLI A +L + L++ G   C ++ C +MI  VD DGDG
Sbjct: 87  RSSSSAAEI-RDAFDLYDQDKNGLISASELHQVLNRLGMS-CSVEDCTRMIGPVDADGDG 146

Query: 164 FVNFQDFRLMMT 176
            VNF++F+ MMT
Sbjct: 147 NVNFEEFQKMMT 154

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BLAST of CSPI06G05810.1 vs. Swiss-Prot
Match: CALM_PYUSP (Calmodulin OS=Pyuridae sp. PE=1 SV=2)

HSP 1 Score: 100.5 bits (249), Expect = 1.9e-20
Identity = 58/140 (41.43%), Postives = 84/140 (60.00%), Query Frame = 1


Query: 41  FQLLFNLLDSDGDGKISMKELSQFLYRLGYKKLKATMEAEEMVKEMDSDRDGFIEMDEFL 100
           F+  F+L D DGDG I+ KEL   +  LG    +A  E ++M+ E+D+D DG I+  EFL
Sbjct: 13  FKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEA--ELQDMINEVDADGDGTIDFPEFL 72

Query: 101 EVL---QKGEEEEEILREAFLIFDGDKNGLICAEKLKRTLSKFGWKKCDLKQCKKMIEGV 160
            ++    K  + EE +REAF +FD D NG I A +L+  ++  G K  D ++  +MI   
Sbjct: 73  TMMARKMKDTDSEEEIREAFRVFDKDGNGFISAAELRHVMTNLGEKLTD-EEVDEMIREA 132

Query: 161 DHDGDGFVNFQDFRLMMTQK 178
           D DGDG VN+++F  MMT K
Sbjct: 133 DIDGDGQVNYEEFVTMMTSK 149

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BLAST of CSPI06G05810.1 vs. Swiss-Prot
Match: CALM_PATSP (Calmodulin OS=Patinopecten sp. PE=1 SV=2)

HSP 1 Score: 98.6 bits (244), Expect = 7.4e-20
Identity = 57/140 (40.71%), Postives = 84/140 (60.00%), Query Frame = 1


Query: 41  FQLLFNLLDSDGDGKISMKELSQFLYRLGYKKLKATMEAEEMVKEMDSDRDGFIEMDEFL 100
           F+  F+L D DGDG I+ KEL   +  LG    +A  E ++M+ E+D+D DG I+  EFL
Sbjct: 13  FKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEA--ELQDMINEVDADGDGTIDFPEFL 72

Query: 101 EVL---QKGEEEEEILREAFLIFDGDKNGLICAEKLKRTLSKFGWKKCDLKQCKKMIEGV 160
            ++    K  + EE +REAF +FD D +G I A +L+  ++  G K  D ++  +MI   
Sbjct: 73  TMMARKMKDTDSEEEIREAFRVFDKDGDGFISAAELRHVMTNLGEKLTD-EEVDEMIREA 132

Query: 161 DHDGDGFVNFQDFRLMMTQK 178
           D DGDG VN+++F  MMT K
Sbjct: 133 DIDGDGQVNYEEFVTMMTSK 149

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BLAST of CSPI06G05810.1 vs. Swiss-Prot
Match: CML2_ARATH (Calmodulin-like protein 2 OS=Arabidopsis thaliana GN=CML2 PE=2 SV=1)

HSP 1 Score: 98.6 bits (244), Expect = 7.4e-20
Identity = 57/135 (42.22%), Postives = 84/135 (62.22%), Query Frame = 1


Query: 44  LFNLLDSDGDGKISMKELSQFLYRLGYKKLKATMEAEEMVKEMDSDRDGFIEMDEFLEVL 103
           +F + D +GDGKI+  EL  F   +G   +    E  EM+ +MD + DG +++DEF  + 
Sbjct: 9   VFQMFDKNGDGKIAKNELKDFFKSVGI--MVPENEINEMIAKMDVNGDGAMDIDEFGSLY 68

Query: 104 QK---GEEEEEILREAFLIFDGDKNGLICAEKLKRTLSKFGWKKC-DLKQCKKMIEGVDH 163
           Q+    +EEEE +REAF +FD + +G I  E+L+  L+  G K+   L+ CKKMI  VD 
Sbjct: 69  QEMVEEKEEEEDMREAFRVFDQNGDGFITDEELRSVLASMGLKQGRTLEDCKKMISKVDV 128

Query: 164 DGDGFVNFQDFRLMM 175
           DGDG VNF++F+ MM
Sbjct: 129 DGDGMVNFKEFKQMM 141

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BLAST of CSPI06G05810.1 vs. TrEMBL
Match: A0A0A0K8Z4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G076830 PE=4 SV=1)

HSP 1 Score: 354.0 bits (907), Expect = 1.1e-94
Identity = 175/178 (98.31%), Postives = 177/178 (99.44%), Query Frame = 1


Query: 1   MFNLTSSLFPTFFHRNISSKPFFQKSLNNQKLPSFTTFNHFQLLFNLLDSDGDGKISMKE 60
           MFNLTSSLFPTFFHRNISSKPFFQKSLNNQKLPSFTTFNHFQLLFNLLDSDGDGKIS KE
Sbjct: 1   MFNLTSSLFPTFFHRNISSKPFFQKSLNNQKLPSFTTFNHFQLLFNLLDSDGDGKISTKE 60

Query: 61  LSQFLYRLGYKKLKATMEAEEMVKEMDSDRDGFIEMDEFLEVLQKGEEEEEILREAFLIF 120
           LSQFLYRLGYKKLKATMEAEEMVKEMDSDRDGFIEMDEFLEVLQKGEEEEEILREAFLIF
Sbjct: 61  LSQFLYRLGYKKLKATMEAEEMVKEMDSDRDGFIEMDEFLEVLQKGEEEEEILREAFLIF 120

Query: 121 DGDKNGLICAEKLKRTLSKFGWKKCDLKQCKKMIEGVDHDGDGFVNFQDFRLMMTQKR 179
           DGDKNGLICAEKLKRTLSKFGW+KCDLKQCKKMIEGVDHDGDG+VNFQDFRLMMTQKR
Sbjct: 121 DGDKNGLICAEKLKRTLSKFGWQKCDLKQCKKMIEGVDHDGDGYVNFQDFRLMMTQKR 178

[Close]

BLAST of CSPI06G05810.1 vs. TrEMBL
Match: A0A067JXS4_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_14512 PE=4 SV=1)

HSP 1 Score: 155.6 bits (392), Expect = 5.6e-35
Identity = 83/164 (50.61%), Postives = 115/164 (70.12%), Query Frame = 1


Query: 15  RNISSKPFFQKSLNNQKLPSFTTF---NHFQLLFNLLDSDGDGKISMKELSQFLYRLGYK 74
           RN+SS  F     +    PSF +    N F+ +FNL+D++GDGKIS  +L + L  LG  
Sbjct: 25  RNLSSSTF-----DFSDTPSFASMEIPNQFKQVFNLIDANGDGKISSYDLIELLLCLGCD 84

Query: 75  KLKATMEAEEMVKEMDSDRDGFIEMDEFLEVLQKGEEEEEILREAFLIFDGDKNGLICAE 134
           K KA  EAE MVKE+D + DGF++ DEF  +L    +EE+ L +AF+IFD DKNGLI A+
Sbjct: 85  KSKAKAEAEGMVKELDRNGDGFVDFDEFFYILNN-YDEEDYLMDAFMIFDADKNGLISAK 144

Query: 135 KLKRTLSKFGWKKCDLKQCKKMIEGVDHDGDGFVNFQDFRLMMT 176
           +L++ LS+ G++KC LK+C+ MI+GVD DGDGFV+F++FR MMT
Sbjct: 145 ELQKVLSRLGFEKCSLKECRIMIKGVDKDGDGFVDFEEFRSMMT 182

[Close]

BLAST of CSPI06G05810.1 vs. TrEMBL
Match: B9RDE4_RICCO (Calmodulin, putative OS=Ricinus communis GN=RCOM_1612330 PE=4 SV=1)

HSP 1 Score: 155.2 bits (391), Expect = 7.4e-35
Identity = 81/154 (52.60%), Postives = 107/154 (69.48%), Query Frame = 1


Query: 33  PSFTTF---NHFQLLFNLLDSDGDGKISMKELSQFLYRLGYKKLKATMEAEEMVKEMDSD 92
           PSF      N F+ +F L+D++GDGKIS  ELS+ L  LG+ K KAT EAE MV+EMD +
Sbjct: 24  PSFAAMEVHNQFKQVFKLIDANGDGKISSCELSELLLCLGFDKSKATSEAEGMVREMDCN 83

Query: 93  RDGFIEMDEFLEVLQK--------GEEEEEILREAFLIFDGDKNGLICAEKLKRTLSKFG 152
            DGF++MDEF+  L          G+ +++ L +AFLIFD DKNGLI AE+LK+ L+  G
Sbjct: 84  GDGFVDMDEFIYTLNDDGKLRGVGGDNKKDYLMDAFLIFDADKNGLISAEELKKVLTNLG 143

Query: 153 WKKCDLKQCKKMIEGVDHDGDGFVNFQDFRLMMT 176
              C LK+C++MI+GVD DGDG VNF++FR MMT
Sbjct: 144 CDNCSLKKCRRMIKGVDKDGDGSVNFEEFRSMMT 177

[Close]

BLAST of CSPI06G05810.1 vs. TrEMBL
Match: W9QZX9_9ROSA (Putative calcium-binding protein CML25 OS=Morus notabilis GN=L484_022442 PE=4 SV=1)

HSP 1 Score: 151.0 bits (380), Expect = 1.4e-33
Identity = 77/149 (51.68%), Postives = 103/149 (69.13%), Query Frame = 1


Query: 39  NHFQLLFNLLDSDGDGKISMKELSQFLYRLGYKKLKATMEAEEMVKEMDSDRDGFIEMDE 98
           N F+ +F ++D +GDGKIS  ELSQ L  LGYKK  AT EAE MV+EMD + DGF+++DE
Sbjct: 55  NQFKQVFEVIDENGDGKISPSELSQVLLCLGYKKSIATKEAEGMVREMDGNGDGFVDLDE 114

Query: 99  FLEVL-----------QKGEEEEEILREAFLIFDGDKNGLICAEKLKRTLSKFGWKKCDL 158
           F++V+             G EE E L +AFL+FD DKNG I  E+L+R L K G   C L
Sbjct: 115 FMDVVINAAASDGISSSGGSEENEHLMDAFLVFDIDKNGKISPEELRRVLVKLGCDNCSL 174

Query: 159 KQCKKMIEGVDHDGDGFVNFQDFRLMMTQ 177
           ++C++MI G D +GDGFV+F++FRLMMT+
Sbjct: 175 RECRRMINGADKNGDGFVDFEEFRLMMTK 203

[Close]

BLAST of CSPI06G05810.1 vs. TrEMBL
Match: B9IC08_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0014s06640g PE=4 SV=2)

HSP 1 Score: 149.8 bits (377), Expect = 3.1e-33
Identity = 75/145 (51.72%), Postives = 101/145 (69.66%), Query Frame = 1


Query: 39  NHFQLLFNLLDSDGDGKISMKELSQFLYRLGYKKLKATMEAEEMVKEMDSDRDGFIEMDE 98
           N F+ +F ++D++GDGKIS  ELS+ L  LGY+K KA  EAE MV+EMD + DGFI++DE
Sbjct: 59  NQFKQVFKVIDANGDGKISCHELSEVLLCLGYEKSKAAWEAERMVREMDCNGDGFIDLDE 118

Query: 99  FLEVLQK-----GEEEEEILREAFLIFDGDKNGLICAEKLKRTLSKFGWKKCDLKQCKKM 158
           F+  +          +E+ L + FLIFD DKNGLI A +L+  L+  G KKC L+ C++M
Sbjct: 119 FINAVNDDGNFGSGNKEDYLMDVFLIFDTDKNGLISARELQTVLTSLGCKKCSLEDCRRM 178

Query: 159 IEGVDHDGDGFVNFQDFRLMMTQKR 179
           I+GVD DGDGFV+F +FR MMT  R
Sbjct: 179 IKGVDKDGDGFVDFHEFRSMMTTNR 203

[Close]

BLAST of CSPI06G05810.1 vs. TAIR10
Match: AT1G24620.1 (AT1G24620.1 EF hand calcium-binding protein family)

HSP 1 Score: 107.8 bits (268), Expect = 6.8e-24
Identity = 59/161 (36.65%), Postives = 97/161 (60.25%), Query Frame = 1


Query: 18  SSKPFFQKSLNNQKLPSFTTFNHFQLLFNLLDSDGDGKISMKELSQFLYRLGYKKLKATM 77
           +  P+ QK+ + +     T     + +F   D +GDGKIS KEL   +  LG++  +   
Sbjct: 20  ADSPYLQKARSGK-----TEIRELEAVFKKFDVNGDGKISSKELGAIMTSLGHEVPEE-- 79

Query: 78  EAEEMVKEMDSDRDGFIEMDEFLEVLQKGEEEEEIL---REAFLIFDGDKNGLICAEKLK 137
           E E+ + E+D   DG+I  +EF+E+  KG ++ ++L   ++AF ++D D NG I AE+L 
Sbjct: 80  ELEKAITEIDRKGDGYINFEEFVELNTKGMDQNDVLENLKDAFSVYDIDGNGSISAEELH 139

Query: 138 RTLSKFGWKKCDLKQCKKMIEGVDHDGDGFVNFQDFRLMMT 176
             L   G  +C + +C+KMI GVD DGDG ++F++F++MMT
Sbjct: 140 EVLRSLG-DECSIAECRKMIGGVDKDGDGTIDFEEFKIMMT 172

[Close]

BLAST of CSPI06G05810.1 vs. TAIR10
Match: AT1G18210.1 (AT1G18210.1 Calcium-binding EF-hand family protein)

HSP 1 Score: 101.7 bits (252), Expect = 4.9e-22
Identity = 56/132 (42.42%), Postives = 84/132 (63.64%), Query Frame = 1


Query: 44  LFNLLDSDGDGKISMKELSQFLYRLGYKKLKATMEAEEMVKEMDSDRDGFIEMDEFLEVL 103
           +F+  DS+GDGKIS+ EL      +G    +   E   +++E+D+DRDG+I +DEF  + 
Sbjct: 27  VFDQFDSNGDGKISVLELGGVFKAMGTSYTET--ELNRVLEEVDTDRDGYINLDEFSTLC 86

Query: 104 QKGEEEEEILREAFLIFDGDKNGLICAEKLKRTLSKFGWKKCDLKQCKKMIEGVDHDGDG 163
           +      EI R+AF ++D DKNGLI A +L + L++ G   C ++ C +MI  VD DGDG
Sbjct: 87  RSSSSAAEI-RDAFDLYDQDKNGLISASELHQVLNRLGMS-CSVEDCTRMIGPVDADGDG 146

Query: 164 FVNFQDFRLMMT 176
            VNF++F+ MMT
Sbjct: 147 NVNFEEFQKMMT 154

[Close]

BLAST of CSPI06G05810.1 vs. TAIR10
Match: AT4G12860.1 (AT4G12860.1 EF hand calcium-binding protein family)

HSP 1 Score: 98.6 bits (244), Expect = 4.1e-21
Identity = 57/135 (42.22%), Postives = 84/135 (62.22%), Query Frame = 1


Query: 44  LFNLLDSDGDGKISMKELSQFLYRLGYKKLKATMEAEEMVKEMDSDRDGFIEMDEFLEVL 103
           +F + D +GDGKI+  EL  F   +G   +    E  EM+ +MD + DG +++DEF  + 
Sbjct: 9   VFQMFDKNGDGKIAKNELKDFFKSVGI--MVPENEINEMIAKMDVNGDGAMDIDEFGSLY 68

Query: 104 QK---GEEEEEILREAFLIFDGDKNGLICAEKLKRTLSKFGWKKC-DLKQCKKMIEGVDH 163
           Q+    +EEEE +REAF +FD + +G I  E+L+  L+  G K+   L+ CKKMI  VD 
Sbjct: 69  QEMVEEKEEEEDMREAFRVFDQNGDGFITDEELRSVLASMGLKQGRTLEDCKKMISKVDV 128

Query: 164 DGDGFVNFQDFRLMM 175
           DGDG VNF++F+ MM
Sbjct: 129 DGDGMVNFKEFKQMM 141

[Close]

BLAST of CSPI06G05810.1 vs. TAIR10
Match: AT1G05990.1 (AT1G05990.1 EF hand calcium-binding protein family)

HSP 1 Score: 97.4 bits (241), Expect = 9.2e-21
Identity = 57/136 (41.91%), Postives = 87/136 (63.97%), Query Frame = 1


Query: 44  LFNLLDSDGDGKISMKELSQFLYRLGYKKLKATMEAEEMVKEMDSDRDGFIEMDEFLE-- 103
           +F + D +GDG I+ KELS+ L  LG        E  +M++++D + DG +++DEF E  
Sbjct: 9   VFQMFDKNGDGTITGKELSETLRSLGI--YIPDKELTQMIEKIDVNGDGCVDIDEFGELY 68

Query: 104 --VLQKGEEEEEILREAFLIFDGDKNGLICAEKLKRTLSKFGWKKCD-LKQCKKMIEGVD 163
             ++ + +EEEE ++EAF +FD + +G I  ++LK  LS  G K+   L  CKKMI+ VD
Sbjct: 69  KTIMDEEDEEEEDMKEAFNVFDQNGDGFITVDELKAVLSSLGLKQGKTLDDCKKMIKKVD 128

Query: 164 HDGDGFVNFQDFRLMM 175
            DGDG VN+++FR MM
Sbjct: 129 VDGDGRVNYKEFRQMM 142

[Close]

BLAST of CSPI06G05810.1 vs. TAIR10
Match: AT3G10190.1 (AT3G10190.1 Calcium-binding EF-hand family protein)

HSP 1 Score: 97.4 bits (241), Expect = 9.2e-21
Identity = 59/150 (39.33%), Postives = 81/150 (54.00%), Query Frame = 1


Query: 31  KLPSFTTFNHFQLLFNLLDSDGDGKISMKELSQFLYRLGYKKLKATMEAEEMVKEMDSDR 90
           ++PS  ++      F L+D D DG +S  +L   L RLG   L    E   M+KE+D D 
Sbjct: 61  EVPSPYSYVEILQAFKLIDRDNDGAVSRHDLESLLSRLGPDPLTEE-EINVMLKEVDCDG 120

Query: 91  DGFIEMDEFLE--VLQKGEEEEEILREAFLIFDGDKNGLICAEKLKRTLSKFGWKKCDLK 150
           DG I ++E     V      +   L+E F  FD D++GLI A++L R  S  G ++C L 
Sbjct: 121 DGTIRLEELASRVVSLDPARDSTELKETFEFFDADRDGLISADELLRVFSTIGDERCTLD 180

Query: 151 QCKKMIEGVDHDGDGFVNFQDFRLMMTQKR 179
            CK+MI  VD DGDGFV F +F  MM  +R
Sbjct: 181 DCKRMIADVDEDGDGFVCFTEFSRMMDLQR 209

[Close]

BLAST of CSPI06G05810.1 vs. NCBI nr
Match: gi|449470826|ref|XP_004153117.1| (PREDICTED: calmodulin-beta-like [Cucumis sativus])

HSP 1 Score: 354.0 bits (907), Expect = 1.6e-94
Identity = 175/178 (98.31%), Postives = 177/178 (99.44%), Query Frame = 1


Query: 1   MFNLTSSLFPTFFHRNISSKPFFQKSLNNQKLPSFTTFNHFQLLFNLLDSDGDGKISMKE 60
           MFNLTSSLFPTFFHRNISSKPFFQKSLNNQKLPSFTTFNHFQLLFNLLDSDGDGKIS KE
Sbjct: 1   MFNLTSSLFPTFFHRNISSKPFFQKSLNNQKLPSFTTFNHFQLLFNLLDSDGDGKISTKE 60

Query: 61  LSQFLYRLGYKKLKATMEAEEMVKEMDSDRDGFIEMDEFLEVLQKGEEEEEILREAFLIF 120
           LSQFLYRLGYKKLKATMEAEEMVKEMDSDRDGFIEMDEFLEVLQKGEEEEEILREAFLIF
Sbjct: 61  LSQFLYRLGYKKLKATMEAEEMVKEMDSDRDGFIEMDEFLEVLQKGEEEEEILREAFLIF 120

Query: 121 DGDKNGLICAEKLKRTLSKFGWKKCDLKQCKKMIEGVDHDGDGFVNFQDFRLMMTQKR 179
           DGDKNGLICAEKLKRTLSKFGW+KCDLKQCKKMIEGVDHDGDG+VNFQDFRLMMTQKR
Sbjct: 121 DGDKNGLICAEKLKRTLSKFGWQKCDLKQCKKMIEGVDHDGDGYVNFQDFRLMMTQKR 178

[Close]

BLAST of CSPI06G05810.1 vs. NCBI nr
Match: gi|659120319|ref|XP_008460131.1| (PREDICTED: calmodulin-beta-like [Cucumis melo])

HSP 1 Score: 339.3 bits (869), Expect = 4.0e-90
Identity = 168/178 (94.38%), Postives = 171/178 (96.07%), Query Frame = 1


Query: 1   MFNLTSSLFPTFFHRNISSKPFFQKSLNNQKLPSFTTFNHFQLLFNLLDSDGDGKISMKE 60
           M NLTSSLF TFFHRNI+SKPFFQKSLNNQKLPSFTTFNHFQLLFN+LDS GDGKIS KE
Sbjct: 1   MLNLTSSLFSTFFHRNITSKPFFQKSLNNQKLPSFTTFNHFQLLFNILDSGGDGKISTKE 60

Query: 61  LSQFLYRLGYKKLKATMEAEEMVKEMDSDRDGFIEMDEFLEVLQKGEEEEEILREAFLIF 120
           LSQFLYRLGYKKLKATMEAEEMVKEMDSDRDGFIEMDEFLEVLQKGEEEEEILREAFLIF
Sbjct: 61  LSQFLYRLGYKKLKATMEAEEMVKEMDSDRDGFIEMDEFLEVLQKGEEEEEILREAFLIF 120

Query: 121 DGDKNGLICAEKLKRTLSKFGWKKCDLKQCKKMIEGVDHDGDGFVNFQDFRLMMTQKR 179
           DGDKNGLICAE+LKRT  KFGW KCDLKQCKKMIEGVDHDGDGFVNFQDFRLMMTQKR
Sbjct: 121 DGDKNGLICAEELKRTFRKFGWTKCDLKQCKKMIEGVDHDGDGFVNFQDFRLMMTQKR 178

[Close]

BLAST of CSPI06G05810.1 vs. NCBI nr
Match: gi|802699492|ref|XP_012083557.1| (PREDICTED: probable calcium-binding protein CML27 [Jatropha curcas])

HSP 1 Score: 155.6 bits (392), Expect = 8.1e-35
Identity = 83/164 (50.61%), Postives = 115/164 (70.12%), Query Frame = 1


Query: 15  RNISSKPFFQKSLNNQKLPSFTTF---NHFQLLFNLLDSDGDGKISMKELSQFLYRLGYK 74
           RN+SS  F     +    PSF +    N F+ +FNL+D++GDGKIS  +L + L  LG  
Sbjct: 25  RNLSSSTF-----DFSDTPSFASMEIPNQFKQVFNLIDANGDGKISSYDLIELLLCLGCD 84

Query: 75  KLKATMEAEEMVKEMDSDRDGFIEMDEFLEVLQKGEEEEEILREAFLIFDGDKNGLICAE 134
           K KA  EAE MVKE+D + DGF++ DEF  +L    +EE+ L +AF+IFD DKNGLI A+
Sbjct: 85  KSKAKAEAEGMVKELDRNGDGFVDFDEFFYILNN-YDEEDYLMDAFMIFDADKNGLISAK 144

Query: 135 KLKRTLSKFGWKKCDLKQCKKMIEGVDHDGDGFVNFQDFRLMMT 176
           +L++ LS+ G++KC LK+C+ MI+GVD DGDGFV+F++FR MMT
Sbjct: 145 ELQKVLSRLGFEKCSLKECRIMIKGVDKDGDGFVDFEEFRSMMT 182

[Close]

BLAST of CSPI06G05810.1 vs. NCBI nr
Match: gi|223548913|gb|EEF50402.1| (calmodulin, putative [Ricinus communis])

HSP 1 Score: 155.2 bits (391), Expect = 1.1e-34
Identity = 81/154 (52.60%), Postives = 107/154 (69.48%), Query Frame = 1


Query: 33  PSFTTF---NHFQLLFNLLDSDGDGKISMKELSQFLYRLGYKKLKATMEAEEMVKEMDSD 92
           PSF      N F+ +F L+D++GDGKIS  ELS+ L  LG+ K KAT EAE MV+EMD +
Sbjct: 24  PSFAAMEVHNQFKQVFKLIDANGDGKISSCELSELLLCLGFDKSKATSEAEGMVREMDCN 83

Query: 93  RDGFIEMDEFLEVLQK--------GEEEEEILREAFLIFDGDKNGLICAEKLKRTLSKFG 152
            DGF++MDEF+  L          G+ +++ L +AFLIFD DKNGLI AE+LK+ L+  G
Sbjct: 84  GDGFVDMDEFIYTLNDDGKLRGVGGDNKKDYLMDAFLIFDADKNGLISAEELKKVLTNLG 143

Query: 153 WKKCDLKQCKKMIEGVDHDGDGFVNFQDFRLMMT 176
              C LK+C++MI+GVD DGDG VNF++FR MMT
Sbjct: 144 CDNCSLKKCRRMIKGVDKDGDGSVNFEEFRSMMT 177

[Close]

BLAST of CSPI06G05810.1 vs. NCBI nr
Match: gi|1000981875|ref|XP_002511733.2| (PREDICTED: probable calcium-binding protein CML18 [Ricinus communis])

HSP 1 Score: 155.2 bits (391), Expect = 1.1e-34
Identity = 81/154 (52.60%), Postives = 107/154 (69.48%), Query Frame = 1


Query: 33  PSFTTF---NHFQLLFNLLDSDGDGKISMKELSQFLYRLGYKKLKATMEAEEMVKEMDSD 92
           PSF      N F+ +F L+D++GDGKIS  ELS+ L  LG+ K KAT EAE MV+EMD +
Sbjct: 45  PSFAAMEVHNQFKQVFKLIDANGDGKISSCELSELLLCLGFDKSKATSEAEGMVREMDCN 104

Query: 93  RDGFIEMDEFLEVLQK--------GEEEEEILREAFLIFDGDKNGLICAEKLKRTLSKFG 152
            DGF++MDEF+  L          G+ +++ L +AFLIFD DKNGLI AE+LK+ L+  G
Sbjct: 105 GDGFVDMDEFIYTLNDDGKLRGVGGDNKKDYLMDAFLIFDADKNGLISAEELKKVLTNLG 164

Query: 153 WKKCDLKQCKKMIEGVDHDGDGFVNFQDFRLMMT 176
              C LK+C++MI+GVD DGDG VNF++FR MMT
Sbjct: 165 CDNCSLKKCRRMIKGVDKDGDGSVNFEEFRSMMT 198

The following BLAST results are available for this feature:

BLAST of CSPI06G05810.1 vs. Swiss-Prot


Match Name	E-value	Identity	Description
CML25_ARATH	1.2e-22	36.65	Probable calcium-binding protein CML25 OS=Arabidopsis thaliana GN=CML25 PE=2 SV=...	[more]
CML27_ARATH	8.7e-21	42.42	Probable calcium-binding protein CML27 OS=Arabidopsis thaliana GN=CML27 PE=1 SV=...	[more]
CALM_PYUSP	1.9e-20	41.43	Calmodulin OS=Pyuridae sp. PE=1 SV=2	[more]
CALM_PATSP	7.4e-20	40.71	Calmodulin OS=Patinopecten sp. PE=1 SV=2	[more]
CML2_ARATH	7.4e-20	42.22	Calmodulin-like protein 2 OS=Arabidopsis thaliana GN=CML2 PE=2 SV=1	[more]

BLAST of CSPI06G05810.1 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K8Z4_CUCSA	1.1e-94	98.31	Uncharacterized protein OS=Cucumis sativus GN=Csa_6G076830 PE=4 SV=1	[more]
A0A067JXS4_JATCU	5.6e-35	50.61	Uncharacterized protein OS=Jatropha curcas GN=JCGZ_14512 PE=4 SV=1	[more]
B9RDE4_RICCO	7.4e-35	52.60	Calmodulin, putative OS=Ricinus communis GN=RCOM_1612330 PE=4 SV=1	[more]
W9QZX9_9ROSA	1.4e-33	51.68	Putative calcium-binding protein CML25 OS=Morus notabilis GN=L484_022442 PE=4 SV...	[more]
B9IC08_POPTR	3.1e-33	51.72	Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0014s06640g PE=4 SV=2	[more]

BLAST of CSPI06G05810.1 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G24620.1	6.8e-24	36.65	EF hand calcium-binding protein family	[more]
AT1G18210.1	4.9e-22	42.42	Calcium-binding EF-hand family protein	[more]
AT4G12860.1	4.1e-21	42.22	EF hand calcium-binding protein family	[more]
AT1G05990.1	9.2e-21	41.91	EF hand calcium-binding protein family	[more]
AT3G10190.1	9.2e-21	39.33	Calcium-binding EF-hand family protein	[more]

BLAST of CSPI06G05810.1 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449470826\|ref\|XP_004153117.1\|	1.6e-94	98.31	PREDICTED: calmodulin-beta-like [Cucumis sativus]	[more]
gi\|659120319\|ref\|XP_008460131.1\|	4.0e-90	94.38	PREDICTED: calmodulin-beta-like [Cucumis melo]	[more]
gi\|802699492\|ref\|XP_012083557.1\|	8.1e-35	50.61	PREDICTED: probable calcium-binding protein CML27 [Jatropha curcas]	[more]
gi\|223548913\|gb\|EEF50402.1\|	1.1e-34	52.60	calmodulin, putative [Ricinus communis]	[more]
gi\|1000981875\|ref\|XP_002511733.2\|	1.1e-34	52.60	PREDICTED: probable calcium-binding protein CML18 [Ricinus communis]	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **INTERPRO**
Term	Definition
IPR002048	EF_hand_dom
IPR011992	EF-hand-dom_pair
IPR018247	EF_Hand_1_Ca_BS

Vocabulary: **Molecular Function**
Term	Definition
GO:0005509	calcium ion binding

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0005509	calcium ion binding

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CSPI06G05810	CSPI06G05810	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CSPI06G05810.1	CSPI06G05810.1-protein	polypeptide

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI06G05810.1.utr3p1	CSPI06G05810.1.utr3p1	three_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI06G05810.1.cds2	CSPI06G05810.1.cds2	CDS
CSPI06G05810.1.cds1	CSPI06G05810.1.cds1	CDS

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI06G05810.1.utr5p1	CSPI06G05810.1.utr5p1	five_prime_UTR

InterPro

Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 42..103 score: 4.3E-12coord: 113..174 score: 2.
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 40..68 score: 0.0026coord: 112..140 score: 0.91coord: 149..177 score: 0.0015coord: 78..106 score: 0
IPR002048	EF-hand domain	PROFILE	PS50222	EF_HAND_2	coord: 145..178 score: 11.584coord: 110..143 score: 10.245coord: 36..71 score: 13.37coord: 74..109 score: 12
IPR011992	EF-hand domain pair	GENE3D	G3DSA:1.10.238.10		coord: 91..175 score: 1.6E-20coord: 40..90 score: 4.9
IPR011992	EF-hand domain pair	unknown	SSF47473	EF-hand	coord: 37..175 score: 2.0
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 49..61 score: -coord: 158..170 score: -coord: 87..99 scor
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 41..176 score: 6.6
None	No IPR available	PANTHER	PTHR10891:SF573	CALCIUM-BINDING PROTEIN CML24-RELATED	coord: 41..176 score: 6.6