CSPI02G27420 (gene) Wild cucumber (PI 183967)

Overview
NameCSPI02G27420
Typegene
OrganismCucumis sativus (Wild cucumber (PI 183967))
DescriptionE3 ubiquitin-protein ligase
LocationChr2 : 23424651 .. 23425357 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TCTGATCTCAATTTCACTTTCAACTCAAATTCATCACTATGTCTTCCCCAACCACTTTCTCCTTCCGATGCTCTGTTCGCTGCAACGCCAACGGCAGTGCTGCCACCAACGGTCAACCCTTGCAATTCGTTGAACTCCGATTGGGGCATTTCAGCCAACTGGTTCTTTCACCTTCTCACCAAATCCTTCAACAAACTTCCCCCATTCTAATTTCTGATACTCTGATCCGTTTCCCTCTGCGGGAGCTTGAAGATCCACCATTCATCTCCCACTCCTCCCTCTGTCGCTTTCTCTCTTCCTACAACATCTCCGACGCCGTCTGTGACGCCATCTTCCTCAAGATCTCTTCATTTGCCTTTCAATTGGTTGCCGGTAACCTCAATTCCAACTTCCACATCATAGCCGCTTTGGATTTTGTGTACACGCACTGGGTGGACCTGGACCCAGCAGAAGATGCAGCGGTGGTGAGGCAAGGGGCGCCGAGGTCGGCGATTGAGAGGTTGATGAAGGAGAAATATGATGGGAACGGAGGGGAAGAGATGGAAGATGAGTGTAGTGTTTGTTATGAAGATCTCCTTGGGAAAACAGAGAAGGAAAAGGAAGTGAGCAGGATCCCATGCGGGCACATGTTTCATAAGTCATGTATTCTCAAATGGCTTAAAATCAGCAATTCATGTCCTTTGTGCAGAGGGGAATTAGAAGCATAG

mRNA sequence

ATGTCTTCCCCAACCACTTTCTCCTTCCGATGCTCTGTTCGCTGCAACGCCAACGGCAGTGCTGCCACCAACGGTCAACCCTTGCAATTCGTTGAACTCCGATTGGGGCATTTCAGCCAACTGGTTCTTTCACCTTCTCACCAAATCCTTCAACAAACTTCCCCCATTCTAATTTCTGATACTCTGATCCGTTTCCCTCTGCGGGAGCTTGAAGATCCACCATTCATCTCCCACTCCTCCCTCTGTCGCTTTCTCTCTTCCTACAACATCTCCGACGCCGTCTGTGACGCCATCTTCCTCAAGATCTCTTCATTTGCCTTTCAATTGGTTGCCGGTAACCTCAATTCCAACTTCCACATCATAGCCGCTTTGGATTTTGTGTACACGCACTGGGTGGACCTGGACCCAGCAGAAGATGCAGCGGTGGTGAGGCAAGGGGCGCCGAGGTCGGCGATTGAGAGGTTGATGAAGGAGAAATATGATGGGAACGGAGGGGAAGAGATGGAAGATGAGTGTAGTGTTTGTTATGAAGATCTCCTTGGGAAAACAGAGAAGGAAAAGGAAGTGAGCAGGATCCCATGCGGGCACATGTTTCATAAGTCATGTATTCTCAAATGGCTTAAAATCAGCAATTCATGTCCTTTGTGCAGAGGGGAATTAGAAGCATAG

Coding sequence (CDS)

ATGTCTTCCCCAACCACTTTCTCCTTCCGATGCTCTGTTCGCTGCAACGCCAACGGCAGTGCTGCCACCAACGGTCAACCCTTGCAATTCGTTGAACTCCGATTGGGGCATTTCAGCCAACTGGTTCTTTCACCTTCTCACCAAATCCTTCAACAAACTTCCCCCATTCTAATTTCTGATACTCTGATCCGTTTCCCTCTGCGGGAGCTTGAAGATCCACCATTCATCTCCCACTCCTCCCTCTGTCGCTTTCTCTCTTCCTACAACATCTCCGACGCCGTCTGTGACGCCATCTTCCTCAAGATCTCTTCATTTGCCTTTCAATTGGTTGCCGGTAACCTCAATTCCAACTTCCACATCATAGCCGCTTTGGATTTTGTGTACACGCACTGGGTGGACCTGGACCCAGCAGAAGATGCAGCGGTGGTGAGGCAAGGGGCGCCGAGGTCGGCGATTGAGAGGTTGATGAAGGAGAAATATGATGGGAACGGAGGGGAAGAGATGGAAGATGAGTGTAGTGTTTGTTATGAAGATCTCCTTGGGAAAACAGAGAAGGAAAAGGAAGTGAGCAGGATCCCATGCGGGCACATGTTTCATAAGTCATGTATTCTCAAATGGCTTAAAATCAGCAATTCATGTCCTTTGTGCAGAGGGGAATTAGAAGCATAG
Homology
[Close]
BLAST of CSPI02G27420 vs. Swiss-Prot
Match: RING1_GOSHI (E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum GN=RING1 PE=1 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 1.9e-09
Identity = 29/74 (39.19%), Postives = 44/74 (59.46%), Query Frame = 1 

Query: 147 APRSAIERLMKEKYDGNGGEEMEDECSVCYEDLLGKTEKEKEVSRIPCGHMFHKSCILKW 206
           A +SAIE L       +      ++C+VC +D     E+  E  ++PC H++HK C+L W
Sbjct: 199 ASKSAIEALPLVNITKSNLNSEFNQCAVCMDDF----EEGTEAKQMPCKHLYHKDCLLPW 258

Query: 207 LKISNSCPLCRGEL 221
           L++ NSCP+CR EL
Sbjct: 259 LELHNSCPVCRHEL 268

[Close]
BLAST of CSPI02G27420 vs. Swiss-Prot
Match: YGI4_SCHPO (Uncharacterized RING finger protein C2A9.04c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=SPBC2A9.04c PE=3 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 2.5e-09
Identity = 27/73 (36.99%), Postives = 47/73 (64.38%), Query Frame = 1 

Query: 152 IERLMKEKYDG----NGGEEMEDECSVCYEDLLGKTEKEKEVSRIPCGHMFHKSCILKWL 211
           ++R +KE +D     +  + M+  C +CY+D+      EK+ +++PCGH+F K+C+ KWL
Sbjct: 83  VKRAVKEAWDSFEPLSNDQLMDLTCPICYDDM--NENDEKQATKMPCGHIFGKNCLQKWL 142

Query: 212 KISNSCPLCRGEL 221
           +   +CPLCR E+
Sbjct: 143 ENHCTCPLCRKEV 153

[Close]
BLAST of CSPI02G27420 vs. Swiss-Prot
Match: RN111_PONAB (E3 ubiquitin-protein ligase Arkadia OS=Pongo abelii GN=RNF111 PE=2 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 3.3e-09
Identity = 30/88 (34.09%), Postives = 50/88 (56.82%), Query Frame = 1 

Query: 137 AEDAAVVRQGAPRSAIERLMKEKYDGNGG--EEMEDECSVCYEDLLGKTEKEKEVSRIPC 196
           A    + R   P    +R +  K DG  G  E+ E++C++C   L    E+ ++V R+PC
Sbjct: 897 ASQGTIERCTYPHKYKKRKLHCKQDGEEGTEEDTEEKCTICLSIL----EEGEDVRRLPC 956

Query: 197 GHMFHKSCILKWLKISNSCPLCRGELEA 223
            H+FH+ C+ +WL  +  CP+CR ++EA
Sbjct: 957 MHLFHQVCVDQWLITNKKCPICRVDIEA 980

[Close]
BLAST of CSPI02G27420 vs. Swiss-Prot
Match: CIP8_ARATH (E3 ubiquitin-protein ligase CIP8 OS=Arabidopsis thaliana GN=CIP8 PE=1 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 3.3e-09
Identity = 35/82 (42.68%), Postives = 50/82 (60.98%), Query Frame = 1 

Query: 144 RQGAP---RSAIERLMKEKYDGNGGEEMEDECSVCYEDL-LGKTEKEKEVSRIPCGHMFH 203
           R+GAP   +SAIE L   +   + GE M   C+VC + + +G+T K     ++PCGH +H
Sbjct: 227 RRGAPPAAKSAIEALETFEVSSSEGE-MVMVCAVCKDGMVMGETGK-----KLPCGHCYH 286

Query: 204 KSCILKWLKISNSCPLCRGELE 222
             CI+ WL   NSCP+CR +LE
Sbjct: 287 GDCIVPWLGTRNSCPVCRFQLE 302

[Close]
BLAST of CSPI02G27420 vs. Swiss-Prot
Match: RN181_DROME (E3 ubiquitin-protein ligase RNF181 homolog OS=Drosophila melanogaster GN=CG7694 PE=2 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 3.3e-09
Identity = 33/79 (41.77%), Postives = 47/79 (59.49%), Query Frame = 1 

Query: 143 VRQGAPRSAIERLMKEKYDGNGGEEMEDECSVCYEDLLGKTEKEKEVSRIPCGHMFHKSC 202
           V + + R+ +E  + E    + G ++E  CSVC E      E+ ++   +PC H FH+ C
Sbjct: 43  VPEASKRAILELPVHEIVKSDEGGDLE--CSVCKEP----AEEGQKYRILPCKHEFHEEC 102

Query: 203 ILKWLKISNSCPLCRGELE 222
           IL WLK +NSCPLCR ELE
Sbjct: 103 ILLWLKKTNSCPLCRYELE 115

[Close]
BLAST of CSPI02G27420 vs. TrEMBL
Match: A0A0A0LRB0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G432790 PE=4 SV=1)

HSP 1 Score: 449.1 bits (1154), Expect = 3.1e-123
Identity = 219/222 (98.65%), Postives = 220/222 (99.10%), Query Frame = 1 

Query: 1   MSSPTTFSFRCSVRCNANGSAATNGQPLQFVELRLGHFSQLVLSPSHQILQQTSPILISD 60
           MSSPTTFSFRCS+RCNANGSAATNGQPLQFVELRLGHFSQLVLSPSHQILQQTSPILISD
Sbjct: 1   MSSPTTFSFRCSIRCNANGSAATNGQPLQFVELRLGHFSQLVLSPSHQILQQTSPILISD 60

Query: 61  TLIRFPLRELEDPPFISHSSLCRFLSSYNISDAVCDAIFLKISSFAFQLVAGNLNSNFHI 120
           TLIRFPLRELEDPPFISHSSLCRFLSSYNISDAVCDAIFLKISSFAFQLVAGNLNSNFHI
Sbjct: 61  TLIRFPLRELEDPPFISHSSLCRFLSSYNISDAVCDAIFLKISSFAFQLVAGNLNSNFHI 120

Query: 121 IAALDFVYTHWVDLDPAEDAAVVRQGAPRSAIERLMKEKYDGNGGEEMEDECSVCYEDLL 180
           IAALDFVYTHWVDLDPAEDAA VRQGAPRSAIERLMKEKYDGNGGEEMEDECSVCYEDL 
Sbjct: 121 IAALDFVYTHWVDLDPAEDAAAVRQGAPRSAIERLMKEKYDGNGGEEMEDECSVCYEDLH 180

Query: 181 GKTEKEKEVSRIPCGHMFHKSCILKWLKISNSCPLCRGELEA 223
           GKTEKEKEVSRIPCGHMFHKSCILKWLKISNSCPLCRGELEA
Sbjct: 181 GKTEKEKEVSRIPCGHMFHKSCILKWLKISNSCPLCRGELEA 222

[Close]
BLAST of CSPI02G27420 vs. TrEMBL
Match: A0A0A0L3Z2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G551140 PE=4 SV=1)

HSP 1 Score: 325.5 bits (833), Expect = 5.1e-86
Identity = 169/230 (73.48%), Postives = 184/230 (80.00%), Query Frame = 1 

Query: 1   MSSPTTFSFRCSVRCNANGSAATNGQPLQFVELRLGHFSQLVLSPSHQILQQTSPILISD 60
           MS P TFSF+CS+R N   S AT+GQPLQFV+LRL HF QLV +PS QILQQTSP LISD
Sbjct: 1   MSLPITFSFQCSIRRNGKSSLATDGQPLQFVQLRLRHFGQLVHTPSQQILQQTSPTLISD 60

Query: 61  TLIRFPLRELEDPPFISHSSLCRFLSSYNISDAVCDAIFLKISSFAFQLVA---GNLNSN 120
           TLI F L ELEDPPFIS+  L RFLSSY+IS AVCDAIF  ISSFA Q++     N+N N
Sbjct: 61  TLIPFSLWELEDPPFISYFFLFRFLSSYDISAAVCDAIFYNISSFAGQILLAADSNINVN 120

Query: 121 FHIIAALDFVYTHWVDLDPAEDAAVVRQGAPRSAIERLMKEKYDGNGGEEME--DECSVC 180
           FHIIA +D VYTHWVDLDPAEDA  VR+GA  S IER+MKEKYDGNGGEE E  D+CSVC
Sbjct: 121 FHIIADVDIVYTHWVDLDPAEDAPAVRKGASTSVIERVMKEKYDGNGGEEKEGDDQCSVC 180

Query: 181 YEDLLGK----TEKEKEVSRIPCGHMFHKSCILKWLKISNSCPLCRGELE 222
           YE L GK     EKEKEVSRIPCGHM+HKSCILKWLKISNSCPLCR ELE
Sbjct: 181 YEQLYGKRENENEKEKEVSRIPCGHMYHKSCILKWLKISNSCPLCRRELE 230

[Close]
BLAST of CSPI02G27420 vs. TrEMBL
Match: A0A0A0LHI3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G889100 PE=4 SV=1)

HSP 1 Score: 199.5 bits (506), Expect = 4.2e-48
Identity = 119/224 (53.12%), Postives = 144/224 (64.29%), Query Frame = 1 

Query: 1   MSSPTTFSFRCSVRCNANGSAATNGQPLQFVELRLGHFSQLVLSPSHQILQQTSPILISD 60
           MSS T  S  C VR     S  T     Q VE+RL  FS+LV   S+Q +QQT P  IS+
Sbjct: 1   MSSTTNLSLDCCVR-----SIPTTADK-QLVEIRLRRFSKLVSPFSNQNIQQTFPTSISN 60

Query: 61  TLIRFPLRELE-DPPFISHSSLCRFLSSYNISDAVCDAIFLKISSFAFQLVAGNLNSNFH 120
            L  FPL ELE + P + H  L +FL     S A   AIF  ISSFA QL AGNLN NFH
Sbjct: 61  ALFHFPLPELELENPSLFHDFLLQFL-----SPADSAAIFHNISSFALQLAAGNLNLNFH 120

Query: 121 IIAALDFVYTHWV--DLDPAEDAAVVRQGAPRSAIERLMKEKY-DGNGGEEMEDECSVCY 180
           +IA +D +YT  +  +L+P + +  VR G P S +ERLM EKY DGNGGE M  +C VCY
Sbjct: 121 LIATVDLLYTISINLNLNPPQYSPPVRNGVPASVMERLMNEKYDDGNGGEGMGGQCCVCY 180

Query: 181 EDLLGKTEKEK-EVSRIPCGHMFHKSCILKWLKISNSCPLCRGE 220
           EDL  + E+EK E +RIPCGH++HKSCILKWL ++NSCPLCR E
Sbjct: 181 EDLNCEREEEKEEATRIPCGHVYHKSCILKWLNVNNSCPLCRSE 213

[Close]
BLAST of CSPI02G27420 vs. TrEMBL
Match: A0A0A0LHS2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G130680 PE=4 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 1.6e-26
Identity = 89/236 (37.71%), Postives = 130/236 (55.08%), Query Frame = 1 

Query: 6   TFSFRCSVRCNANGSAATNGQPLQFVELRLGHFSQLV---LSPS---HQILQQTSPILIS 65
           +FS    V+C+ +G   T+    Q + +RL   S+L+   L+P      IL+QT   LI 
Sbjct: 2   SFSATSCVQCSFSGYPNTD----QTLIVRLRFCSRLISQSLAPEIVPDVILEQTPFTLIG 61

Query: 66  DTL-----IRFPLRELEDPPFISHSSLCRFLSSYNISDAVCDAIFLKISSFAFQLVAGNL 125
           + L     +++PL++ E    ++      FL S+N+  +VC  +   I SFA Q    N 
Sbjct: 62  EALFSLSNLQYPLQQQELEYMVNE-----FLRSFNLRSSVCRIVSHNILSFAQQFDINN- 121

Query: 126 NSNFHIIAALDFVYTHWVDLDP-------AEDAAVVRQGAPRSAIERLMKEKYDGNGGEE 185
              F I   +D++  +W +  P         +  +  +GA  SAIER+ K+K+DG  GEE
Sbjct: 122 -ECFKIETNVDYIKEYWTEAAPELYPISTVGEEGLPPRGASESAIERVKKQKFDGFRGEE 181

Query: 186 MED--ECSVCYEDLLGKTEKEKEVSRIPCGHMFHKSCILKWLKISNSCPLCRGELE 222
            E+  +CSVC E++     K  EV RIPCGH++HKSCILKWL+ISNSCPLCR  LE
Sbjct: 182 EEEIGDCSVCCEEI---KRKGGEVRRIPCGHVYHKSCILKWLEISNSCPLCRAALE 223

[Close]
BLAST of CSPI02G27420 vs. TrEMBL
Match: A0A0A0K2W2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G025210 PE=4 SV=1)

HSP 1 Score: 107.1 bits (266), Expect = 2.9e-20
Identity = 74/205 (36.10%), Postives = 108/205 (52.68%), Query Frame = 1 

Query: 38  FSQLVLSPS------HQILQQTSPILISDTLIRFPLRELEDPPFISHSSLCRFLSSYNIS 97
           F Q ++S S      H++L QT  I   + L    LR+L+ P F     + + LSS NI 
Sbjct: 30  FIQQIISVSPLTGRPHEVLHQTPRIPYHNALFYLSLRQLQSPLFY----ITQILSSLNIR 89

Query: 98  DAVCDAIFLKISSFAFQLVAGNLNSNFHIIAALDFVYTHW--------------VDLDPA 157
            A    +  +I+S   Q+   N  +NFHI+A +DF+   W              V LD A
Sbjct: 90  PAASQNMARRIASHILQMPDRNSETNFHILAEVDFIRLIWLPSMGGGGAGAEETVVLDEA 149

Query: 158 EDAAVVRQGAPRSAIERLMKEKYDGNGGEEMED--ECSVCYEDLLGKTEKEKEVSRIPCG 217
             A  V++G   +  ERL  E       E+ME+  +CS+C ++L   + +++EV RIPCG
Sbjct: 150 PPA--VKRGVGVARGERLRSE-------EKMEELGDCSICLDEL---SCEKREVMRIPCG 209

Query: 218 HMFHKSCILKWLKISNSCPLCRGEL 221
           H++H+SCI KWL+  NSCPLCR  L
Sbjct: 210 HVYHESCIFKWLENHNSCPLCRKPL 218

[Close]
BLAST of CSPI02G27420 vs. TAIR10
Match: AT4G05350.1 (AT4G05350.1 RING/U-box superfamily protein)

HSP 1 Score: 65.9 bits (159), Expect = 3.7e-11
Identity = 43/137 (31.39%), Postives = 66/137 (48.18%), Query Frame = 1 

Query: 85  LSSYNISDAVCDAIFLKISSFAFQLVAGNLNSNFHIIAALDFVYTHWVDLDPAEDAAVVR 144
           L  Y     +CD +  KIS+ A  L  G+  + F +  A    Y            +V+ 
Sbjct: 80  LVGYPFESYLCDDLAPKISTAAIDLGFGH--NGFTLTMAATVTY---------RTVSVMS 139

Query: 145 QGAPRSAIERLMKEKYDGNGGEEMEDE-CSVCYEDLLGKTEKEKEVSRIPCGHMFHKSCI 204
                  +  L + K +     +ME E CS+C E L+    K ++V+R+ C H+FH  C+
Sbjct: 140 NDEKSLRMVLLGRMKAEEFKSLKMETEPCSICLESLVSGP-KPRDVTRMTCSHVFHNGCL 199

Query: 205 LKWLKISNSCPLCRGEL 221
           L+WLK  N+CPLCR E+
Sbjct: 200 LEWLKRKNTCPLCRTEI 204

[Close]
BLAST of CSPI02G27420 vs. TAIR10
Match: AT4G12190.1 (AT4G12190.1 RING/U-box superfamily protein)

HSP 1 Score: 64.7 bits (156), Expect = 8.3e-11
Identity = 27/55 (49.09%), Postives = 39/55 (70.91%), Query Frame = 1 

Query: 167 EMEDE-CSVCYEDLLGKTEKEKEVSRIPCGHMFHKSCILKWLKISNSCPLCRGEL 221
           +ME E CS+C E L+    K ++++R+ C H+FH  C+L+WLK  N+CPLCR EL
Sbjct: 16  KMETEPCSICLESLVSGP-KPRDITRMTCSHVFHNGCLLEWLKRKNTCPLCRTEL 69

[Close]
BLAST of CSPI02G27420 vs. TAIR10
Match: AT5G56340.1 (AT5G56340.1 RING/U-box superfamily protein)

HSP 1 Score: 63.9 bits (154), Expect = 1.4e-10
Identity = 40/108 (37.04%), Postives = 54/108 (50.00%), Query Frame = 1 

Query: 117 NFHIIAALDFVYTHWVDLDPAEDAAVVRQGAP---RSAIERLMKEKYDGNGGEEMED-EC 176
           ++ I   LD +  H  + DP       RQG P   + A+E L   K        ME  +C
Sbjct: 213 DYFIGPGLDLLLQHLAENDPN------RQGTPPARKEAVEALPTVKI-------MEPLQC 272

Query: 177 SVCYEDLLGKTEKEKEVSRIPCGHMFHKSCILKWLKISNSCPLCRGEL 221
           SVC +D     EK  E   +PC H FH  CI+ WL++ +SCP+CR EL
Sbjct: 273 SVCLDDF----EKGTEAKEMPCKHKFHVRCIVPWLELHSSCPVCRFEL 303

[Close]
BLAST of CSPI02G27420 vs. TAIR10
Match: AT5G37230.1 (AT5G37230.1 RING/U-box superfamily protein)

HSP 1 Score: 63.5 bits (153), Expect = 1.8e-10
Identity = 45/145 (31.03%), Postives = 62/145 (42.76%), Query Frame = 1 

Query: 75  FISHSSLCRFLSSYNISDAVCDAIFLKISSFAFQLVAGNLNSNFHIIAALDFVYTHWVDL 134
           FI+H+  CR LS   + D                          HI       +   V  
Sbjct: 81  FITHTEFCRLLSERIVLDCA------------------------HIPQPFSVSFYVRVTR 140

Query: 135 DPAEDAAVVRQGAPRSAIERLMKEKYDG--NGGEEMEDECSVCYEDLLGKTEKEKEVSRI 194
           D    + VVR    R   +RL++E+     N G+E E  CS+C ED   ++  +  +   
Sbjct: 141 DVMLPSIVVRS---RDMFQRLLEEQTMELTNLGDEEETTCSICMEDF-SESHDDNIILLP 197

Query: 195 PCGHMFHKSCILKWLKISNSCPLCR 218
            C H+FH+SCI KWLK   SCPLCR
Sbjct: 201 DCFHLFHQSCIFKWLKRQRSCPLCR 197

[Close]
BLAST of CSPI02G27420 vs. TAIR10
Match: AT5G64920.1 (AT5G64920.1 COP1-interacting protein 8)

HSP 1 Score: 63.5 bits (153), Expect = 1.8e-10
Identity = 35/82 (42.68%), Postives = 50/82 (60.98%), Query Frame = 1 

Query: 144 RQGAP---RSAIERLMKEKYDGNGGEEMEDECSVCYEDL-LGKTEKEKEVSRIPCGHMFH 203
           R+GAP   +SAIE L   +   + GE M   C+VC + + +G+T K     ++PCGH +H
Sbjct: 227 RRGAPPAAKSAIEALETFEVSSSEGE-MVMVCAVCKDGMVMGETGK-----KLPCGHCYH 286

Query: 204 KSCILKWLKISNSCPLCRGELE 222
             CI+ WL   NSCP+CR +LE
Sbjct: 287 GDCIVPWLGTRNSCPVCRFQLE 302

[Close]
BLAST of CSPI02G27420 vs. NCBI nr
Match: gi|778674460|ref|XP_011650219.1| (PREDICTED: RING-H2 finger protein ATL16-like [Cucumis sativus])

HSP 1 Score: 449.1 bits (1154), Expect = 4.4e-123
Identity = 219/222 (98.65%), Postives = 220/222 (99.10%), Query Frame = 1 

Query: 1   MSSPTTFSFRCSVRCNANGSAATNGQPLQFVELRLGHFSQLVLSPSHQILQQTSPILISD 60
           MSSPTTFSFRCS+RCNANGSAATNGQPLQFVELRLGHFSQLVLSPSHQILQQTSPILISD
Sbjct: 1   MSSPTTFSFRCSIRCNANGSAATNGQPLQFVELRLGHFSQLVLSPSHQILQQTSPILISD 60

Query: 61  TLIRFPLRELEDPPFISHSSLCRFLSSYNISDAVCDAIFLKISSFAFQLVAGNLNSNFHI 120
           TLIRFPLRELEDPPFISHSSLCRFLSSYNISDAVCDAIFLKISSFAFQLVAGNLNSNFHI
Sbjct: 61  TLIRFPLRELEDPPFISHSSLCRFLSSYNISDAVCDAIFLKISSFAFQLVAGNLNSNFHI 120

Query: 121 IAALDFVYTHWVDLDPAEDAAVVRQGAPRSAIERLMKEKYDGNGGEEMEDECSVCYEDLL 180
           IAALDFVYTHWVDLDPAEDAA VRQGAPRSAIERLMKEKYDGNGGEEMEDECSVCYEDL 
Sbjct: 121 IAALDFVYTHWVDLDPAEDAAAVRQGAPRSAIERLMKEKYDGNGGEEMEDECSVCYEDLH 180

Query: 181 GKTEKEKEVSRIPCGHMFHKSCILKWLKISNSCPLCRGELEA 223
           GKTEKEKEVSRIPCGHMFHKSCILKWLKISNSCPLCRGELEA
Sbjct: 181 GKTEKEKEVSRIPCGHMFHKSCILKWLKISNSCPLCRGELEA 222

[Close]
BLAST of CSPI02G27420 vs. NCBI nr
Match: gi|700199693|gb|KGN54851.1| (hypothetical protein Csa_4G551140 [Cucumis sativus])

HSP 1 Score: 325.5 bits (833), Expect = 7.4e-86
Identity = 169/230 (73.48%), Postives = 184/230 (80.00%), Query Frame = 1 

Query: 1   MSSPTTFSFRCSVRCNANGSAATNGQPLQFVELRLGHFSQLVLSPSHQILQQTSPILISD 60
           MS P TFSF+CS+R N   S AT+GQPLQFV+LRL HF QLV +PS QILQQTSP LISD
Sbjct: 1   MSLPITFSFQCSIRRNGKSSLATDGQPLQFVQLRLRHFGQLVHTPSQQILQQTSPTLISD 60

Query: 61  TLIRFPLRELEDPPFISHSSLCRFLSSYNISDAVCDAIFLKISSFAFQLVA---GNLNSN 120
           TLI F L ELEDPPFIS+  L RFLSSY+IS AVCDAIF  ISSFA Q++     N+N N
Sbjct: 61  TLIPFSLWELEDPPFISYFFLFRFLSSYDISAAVCDAIFYNISSFAGQILLAADSNINVN 120

Query: 121 FHIIAALDFVYTHWVDLDPAEDAAVVRQGAPRSAIERLMKEKYDGNGGEEME--DECSVC 180
           FHIIA +D VYTHWVDLDPAEDA  VR+GA  S IER+MKEKYDGNGGEE E  D+CSVC
Sbjct: 121 FHIIADVDIVYTHWVDLDPAEDAPAVRKGASTSVIERVMKEKYDGNGGEEKEGDDQCSVC 180

Query: 181 YEDLLGK----TEKEKEVSRIPCGHMFHKSCILKWLKISNSCPLCRGELE 222
           YE L GK     EKEKEVSRIPCGHM+HKSCILKWLKISNSCPLCR ELE
Sbjct: 181 YEQLYGKRENENEKEKEVSRIPCGHMYHKSCILKWLKISNSCPLCRRELE 230

[Close]
BLAST of CSPI02G27420 vs. NCBI nr
Match: gi|659132530|ref|XP_008466247.1| (PREDICTED: uncharacterized protein LOC103503717 [Cucumis melo])

HSP 1 Score: 221.1 bits (562), Expect = 2.0e-54
Identity = 129/226 (57.08%), Postives = 151/226 (66.81%), Query Frame = 1 

Query: 1   MSSPTTFSFRCSVRCNANGSAATNGQPLQFVELRLGHFSQLVLSPSHQILQQTSPILISD 60
           MSS TT S  C +    + S AT     Q VE+RL  +S+LV   S+QI+QQT P  ISD
Sbjct: 1   MSSTTTLSLECCI----HSSVATTADE-QLVEIRLRRYSKLVSPSSNQIIQQTFPTPISD 60

Query: 61  TLIRFPLRELE--DPPFISHSSLCRFLSSYNISDAVCDAIFLKISSFAFQLVAGNLNSNF 120
            L  FPL ELE  +PPF  H  L +FLS      A   AIF  ISSFA QL AGNLN NF
Sbjct: 61  ALFHFPLPELELENPPFFFHDLLLQFLSP-----ADSTAIFHNISSFALQLAAGNLNLNF 120

Query: 121 HIIAALDFVYTHWVDLD--PAEDAAVVRQGAPRSAIERLMKEKYD-GNGGEEMEDECSVC 180
           H+IA +D +YT  VDLD  P + + +VR GAP S IERLM EKYD GNG EEM D+C VC
Sbjct: 121 HLIATVDLLYTISVDLDLDPPQYSPLVRNGAPASVIERLMDEKYDDGNGREEMGDQCCVC 180

Query: 181 YEDLL--GKTEKEKEVSRIPCGHMFHKSCILKWLKISNSCPLCRGE 220
           YEDL    + EKE+EV+RIPCGH++HK CILKWL I+NSCPLCR E
Sbjct: 181 YEDLNCEREEEKEEEVTRIPCGHVYHKYCILKWLNINNSCPLCRRE 216

[Close]
BLAST of CSPI02G27420 vs. NCBI nr
Match: gi|778689036|ref|XP_011652885.1| (PREDICTED: RING-H2 finger protein ATL66-like [Cucumis sativus])

HSP 1 Score: 199.5 bits (506), Expect = 6.1e-48
Identity = 119/224 (53.12%), Postives = 144/224 (64.29%), Query Frame = 1 

Query: 1   MSSPTTFSFRCSVRCNANGSAATNGQPLQFVELRLGHFSQLVLSPSHQILQQTSPILISD 60
           MSS T  S  C VR     S  T     Q VE+RL  FS+LV   S+Q +QQT P  IS+
Sbjct: 1   MSSTTNLSLDCCVR-----SIPTTADK-QLVEIRLRRFSKLVSPFSNQNIQQTFPTSISN 60

Query: 61  TLIRFPLRELE-DPPFISHSSLCRFLSSYNISDAVCDAIFLKISSFAFQLVAGNLNSNFH 120
            L  FPL ELE + P + H  L +FL     S A   AIF  ISSFA QL AGNLN NFH
Sbjct: 61  ALFHFPLPELELENPSLFHDFLLQFL-----SPADSAAIFHNISSFALQLAAGNLNLNFH 120

Query: 121 IIAALDFVYTHWV--DLDPAEDAAVVRQGAPRSAIERLMKEKY-DGNGGEEMEDECSVCY 180
           +IA +D +YT  +  +L+P + +  VR G P S +ERLM EKY DGNGGE M  +C VCY
Sbjct: 121 LIATVDLLYTISINLNLNPPQYSPPVRNGVPASVMERLMNEKYDDGNGGEGMGGQCCVCY 180

Query: 181 EDLLGKTEKEK-EVSRIPCGHMFHKSCILKWLKISNSCPLCRGE 220
           EDL  + E+EK E +RIPCGH++HKSCILKWL ++NSCPLCR E
Sbjct: 181 EDLNCEREEEKEEATRIPCGHVYHKSCILKWLNVNNSCPLCRSE 213

[Close]
BLAST of CSPI02G27420 vs. NCBI nr
Match: gi|659121671|ref|XP_008460768.1| (PREDICTED: E3 ubiquitin-protein ligase RING1-like [Cucumis melo])

HSP 1 Score: 130.6 bits (327), Expect = 3.5e-27
Identity = 91/225 (40.44%), Postives = 123/225 (54.67%), Query Frame = 1 

Query: 1   MSSPTTFSFRCSVRCNANGSAATNGQPLQFVELRLGHFSQLVLSPSHQILQQTSPILISD 60
           MS  T+ SF+CS     N           F+  R G+ S+L++    QILQQT  IL+ +
Sbjct: 1   MSLSTSSSFQCSFSATPNTD--------HFLICRFGYSSRLIIVD--QILQQTPSILMGE 60

Query: 61  TLIRFPLRELEDPPFISHSSLCRFLSSYNISD--AVCDAIFLKISSFAFQLVAGNLNSNF 120
           TLI F      +        +  FL S+N+    +    I  KI SFA Q    N++ +F
Sbjct: 61  TLIPFSNLWHPEQQQSIEFLVNEFLGSFNLHSFASARRTICRKILSFALQF--HNVHGSF 120

Query: 121 HIIAALDFVYTHWVDLDPAEDAAVVRQ--GAPRSAIERLMKEKYDGNGGEEMEDECSVCY 180
            I A ++ V    +     ++     +  GAP SAIERL KEK+D    EE   +CSVCY
Sbjct: 121 KIEANVEHVEDLIIGEQGFQETPSPPRTTGAPESAIERLRKEKFDVLNKEEEIGDCSVCY 180

Query: 181 EDLLGKTEKEKEVSRIPCGHMFHKSCILKWLKISNSCPLCRGELE 222
           E+L G+    KEVSRIPCGH++HKSCIL WL+I+NSCPLCR  L+
Sbjct: 181 EELKGQG---KEVSRIPCGHVYHKSCILTWLQINNSCPLCRAPLQ 210

The following BLAST results are available for this feature:
BLAST of CSPI02G27420 vs. Swiss-Prot
Match NameE-valueIdentityDescription
RING1_GOSHI1.9e-0939.19E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum GN=RING1 PE=1 SV=1[more]
YGI4_SCHPO2.5e-0936.99Uncharacterized RING finger protein C2A9.04c OS=Schizosaccharomyces pombe (strai... [more]
RN111_PONAB3.3e-0934.09E3 ubiquitin-protein ligase Arkadia OS=Pongo abelii GN=RNF111 PE=2 SV=1[more]
CIP8_ARATH3.3e-0942.68E3 ubiquitin-protein ligase CIP8 OS=Arabidopsis thaliana GN=CIP8 PE=1 SV=1[more]
RN181_DROME3.3e-0941.77E3 ubiquitin-protein ligase RNF181 homolog OS=Drosophila melanogaster GN=CG7694 ... [more]
BLAST of CSPI02G27420 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LRB0_CUCSA3.1e-12398.65Uncharacterized protein OS=Cucumis sativus GN=Csa_2G432790 PE=4 SV=1[more]
A0A0A0L3Z2_CUCSA5.1e-8673.48Uncharacterized protein OS=Cucumis sativus GN=Csa_4G551140 PE=4 SV=1[more]
A0A0A0LHI3_CUCSA4.2e-4853.13Uncharacterized protein OS=Cucumis sativus GN=Csa_3G889100 PE=4 SV=1[more]
A0A0A0LHS2_CUCSA1.6e-2637.71Uncharacterized protein OS=Cucumis sativus GN=Csa_2G130680 PE=4 SV=1[more]
A0A0A0K2W2_CUCSA2.9e-2036.10Uncharacterized protein OS=Cucumis sativus GN=Csa_7G025210 PE=4 SV=1[more]
BLAST of CSPI02G27420 vs. TAIR10
Match NameE-valueIdentityDescription
AT4G05350.13.7e-1131.39 RING/U-box superfamily protein[more]
AT4G12190.18.3e-1149.09 RING/U-box superfamily protein[more]
AT5G56340.11.4e-1037.04 RING/U-box superfamily protein[more]
AT5G37230.11.8e-1031.03 RING/U-box superfamily protein[more]
AT5G64920.11.8e-1042.68 COP1-interacting protein 8[more]
BLAST of CSPI02G27420 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|778674460|ref|XP_011650219.1|4.4e-12398.65PREDICTED: RING-H2 finger protein ATL16-like [Cucumis sativus][more]
gi|700199693|gb|KGN54851.1|7.4e-8673.48hypothetical protein Csa_4G551140 [Cucumis sativus][more]
gi|659132530|ref|XP_008466247.1|2.0e-5457.08PREDICTED: uncharacterized protein LOC103503717 [Cucumis melo][more]
gi|778689036|ref|XP_011652885.1|6.1e-4853.13PREDICTED: RING-H2 finger protein ATL66-like [Cucumis sativus][more]
gi|659121671|ref|XP_008460768.1|3.5e-2740.44PREDICTED: E3 ubiquitin-protein ligase RING1-like [Cucumis melo][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR001841Znf_RING
IPR013083Znf_RING/FYVE/PHD
Vocabulary: Molecular Function
TermDefinition
GO:0005515protein binding
GO:0008270zinc ion binding
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0005515 protein binding
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0003674 molecular_function
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI02G27420.1CSPI02G27420.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 170..217
score: 3.3
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 172..216
score: 1.
IPR001841Zinc finger, RING-typePROFILEPS50089ZF_RING_2coord: 172..217
score: 13
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3DG3DSA:3.30.40.10coord: 168..220
score: 1.2
NoneNo IPR availablePANTHERPTHR22763RING ZINC FINGER PROTEINcoord: 145..221
score: 1.1
NoneNo IPR availableunknownSSF57850RING/U-boxcoord: 166..220
score: 1.15

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
CSPI02G27420Csa2G432790Cucumber (Chinese Long) v2cpicuB065
CSPI02G27420CsaV3_2G036020Cucumber (Chinese Long) v3cpicucB081
CSPI02G27420CsGy2G026850Cucumber (Gy14) v2cgybcpiB057
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
CSPI02G27420Cucurbita pepo (Zucchini)cpecpiB272
CSPI02G27420Cucurbita pepo (Zucchini)cpecpiB708
CSPI02G27420Bottle gourd (USVL1VR-Ls)cpilsiB092
CSPI02G27420Melon (DHL92) v3.6.1cpimedB088
CSPI02G27420Silver-seed gourdcarcpiB0991
CSPI02G27420Silver-seed gourdcarcpiB1089
CSPI02G27420Watermelon (97103) v2cpiwmbB121
CSPI02G27420Wax gourdcpiwgoB171
CSPI02G27420Wax gourdcpiwgoB186
CSPI02G27420Cucumber (Gy14) v1cgycpiB396
CSPI02G27420Cucurbita maxima (Rimu)cmacpiB549
CSPI02G27420Cucurbita maxima (Rimu)cmacpiB595
CSPI02G27420Cucurbita moschata (Rifu)cmocpiB538
CSPI02G27420Cucurbita moschata (Rifu)cmocpiB584
CSPI02G27420Melon (DHL92) v3.5.1cpimeB096
CSPI02G27420Watermelon (Charleston Gray)cpiwcgB129
CSPI02G27420Watermelon (97103) v1cpiwmB145