CSPI02G27420.1 (mRNA) Wild cucumber (PI 183967) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CSPI02G27420.1
Type	mRNA
Organism	Cucumis sativus (Wild cucumber (PI 183967))
Description	E3 ubiquitin-protein ligase
Location	Chr2 : 23424651 .. 23425357 (-)
Sequence length	669

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

TCTGATCTCAATTTCACTTTCAACTCAAATTCATCACTATGTCTTCCCCAACCACTTTCTCCTTCCGATGCTCTGTTCGCTGCAACGCCAACGGCAGTGCTGCCACCAACGGTCAACCCTTGCAATTCGTTGAACTCCGATTGGGGCATTTCAGCCAACTGGTTCTTTCACCTTCTCACCAAATCCTTCAACAAACTTCCCCCATTCTAATTTCTGATACTCTGATCCGTTTCCCTCTGCGGGAGCTTGAAGATCCACCATTCATCTCCCACTCCTCCCTCTGTCGCTTTCTCTCTTCCTACAACATCTCCGACGCCGTCTGTGACGCCATCTTCCTCAAGATCTCTTCATTTGCCTTTCAATTGGTTGCCGGTAACCTCAATTCCAACTTCCACATCATAGCCGCTTTGGATTTTGTGTACACGCACTGGGTGGACCTGGACCCAGCAGAAGATGCAGCGGTGGTGAGGCAAGGGGCGCCGAGGTCGGCGATTGAGAGGTTGATGAAGGAGAAATATGATGGGAACGGAGGGGAAGAGATGGAAGATGAGTGTAGTGTTTGTTATGAAGATCTCCTTGGGAAAACAGAGAAGGAAAAGGAAGTGAGCAGGATCCCATGCGGGCACATGTTTCATAAGTCATGTATTCTCAAATGGCTTAAAATCAGCAATTCATGTCCTTTGTGCAGAGGGGAATTAGAAGCATAG

mRNA sequence

ATGTCTTCCCCAACCACTTTCTCCTTCCGATGCTCTGTTCGCTGCAACGCCAACGGCAGTGCTGCCACCAACGGTCAACCCTTGCAATTCGTTGAACTCCGATTGGGGCATTTCAGCCAACTGGTTCTTTCACCTTCTCACCAAATCCTTCAACAAACTTCCCCCATTCTAATTTCTGATACTCTGATCCGTTTCCCTCTGCGGGAGCTTGAAGATCCACCATTCATCTCCCACTCCTCCCTCTGTCGCTTTCTCTCTTCCTACAACATCTCCGACGCCGTCTGTGACGCCATCTTCCTCAAGATCTCTTCATTTGCCTTTCAATTGGTTGCCGGTAACCTCAATTCCAACTTCCACATCATAGCCGCTTTGGATTTTGTGTACACGCACTGGGTGGACCTGGACCCAGCAGAAGATGCAGCGGTGGTGAGGCAAGGGGCGCCGAGGTCGGCGATTGAGAGGTTGATGAAGGAGAAATATGATGGGAACGGAGGGGAAGAGATGGAAGATGAGTGTAGTGTTTGTTATGAAGATCTCCTTGGGAAAACAGAGAAGGAAAAGGAAGTGAGCAGGATCCCATGCGGGCACATGTTTCATAAGTCATGTATTCTCAAATGGCTTAAAATCAGCAATTCATGTCCTTTGTGCAGAGGGGAATTAGAAGCATAG

Coding sequence (CDS)

ATGTCTTCCCCAACCACTTTCTCCTTCCGATGCTCTGTTCGCTGCAACGCCAACGGCAGTGCTGCCACCAACGGTCAACCCTTGCAATTCGTTGAACTCCGATTGGGGCATTTCAGCCAACTGGTTCTTTCACCTTCTCACCAAATCCTTCAACAAACTTCCCCCATTCTAATTTCTGATACTCTGATCCGTTTCCCTCTGCGGGAGCTTGAAGATCCACCATTCATCTCCCACTCCTCCCTCTGTCGCTTTCTCTCTTCCTACAACATCTCCGACGCCGTCTGTGACGCCATCTTCCTCAAGATCTCTTCATTTGCCTTTCAATTGGTTGCCGGTAACCTCAATTCCAACTTCCACATCATAGCCGCTTTGGATTTTGTGTACACGCACTGGGTGGACCTGGACCCAGCAGAAGATGCAGCGGTGGTGAGGCAAGGGGCGCCGAGGTCGGCGATTGAGAGGTTGATGAAGGAGAAATATGATGGGAACGGAGGGGAAGAGATGGAAGATGAGTGTAGTGTTTGTTATGAAGATCTCCTTGGGAAAACAGAGAAGGAAAAGGAAGTGAGCAGGATCCCATGCGGGCACATGTTTCATAAGTCATGTATTCTCAAATGGCTTAAAATCAGCAATTCATGTCCTTTGTGCAGAGGGGAATTAGAAGCATAG

Homology

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BLAST of CSPI02G27420.1 vs. Swiss-Prot
Match: RING1_GOSHI (E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum GN=RING1 PE=1 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 1.9e-09
Identity = 29/74 (39.19%), Postives = 44/74 (59.46%), Query Frame = 1


Query: 147 APRSAIERLMKEKYDGNGGEEMEDECSVCYEDLLGKTEKEKEVSRIPCGHMFHKSCILKW 206
           A +SAIE L       +      ++C+VC +D     E+  E  ++PC H++HK C+L W
Sbjct: 199 ASKSAIEALPLVNITKSNLNSEFNQCAVCMDDF----EEGTEAKQMPCKHLYHKDCLLPW 258

Query: 207 LKISNSCPLCRGEL 221
           L++ NSCP+CR EL
Sbjct: 259 LELHNSCPVCRHEL 268

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BLAST of CSPI02G27420.1 vs. Swiss-Prot
Match: YGI4_SCHPO (Uncharacterized RING finger protein C2A9.04c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=SPBC2A9.04c PE=3 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 2.5e-09
Identity = 27/73 (36.99%), Postives = 47/73 (64.38%), Query Frame = 1


Query: 152 IERLMKEKYDG----NGGEEMEDECSVCYEDLLGKTEKEKEVSRIPCGHMFHKSCILKWL 211
           ++R +KE +D     +  + M+  C +CY+D+      EK+ +++PCGH+F K+C+ KWL
Sbjct: 83  VKRAVKEAWDSFEPLSNDQLMDLTCPICYDDM--NENDEKQATKMPCGHIFGKNCLQKWL 142

Query: 212 KISNSCPLCRGEL 221
           +   +CPLCR E+
Sbjct: 143 ENHCTCPLCRKEV 153

[Close]

BLAST of CSPI02G27420.1 vs. Swiss-Prot
Match: RN111_PONAB (E3 ubiquitin-protein ligase Arkadia OS=Pongo abelii GN=RNF111 PE=2 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 3.3e-09
Identity = 30/88 (34.09%), Postives = 50/88 (56.82%), Query Frame = 1


Query: 137 AEDAAVVRQGAPRSAIERLMKEKYDGNGG--EEMEDECSVCYEDLLGKTEKEKEVSRIPC 196
           A    + R   P    +R +  K DG  G  E+ E++C++C   L    E+ ++V R+PC
Sbjct: 897 ASQGTIERCTYPHKYKKRKLHCKQDGEEGTEEDTEEKCTICLSIL----EEGEDVRRLPC 956

Query: 197 GHMFHKSCILKWLKISNSCPLCRGELEA 223
            H+FH+ C+ +WL  +  CP+CR ++EA
Sbjct: 957 MHLFHQVCVDQWLITNKKCPICRVDIEA 980

[Close]

BLAST of CSPI02G27420.1 vs. Swiss-Prot
Match: CIP8_ARATH (E3 ubiquitin-protein ligase CIP8 OS=Arabidopsis thaliana GN=CIP8 PE=1 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 3.3e-09
Identity = 35/82 (42.68%), Postives = 50/82 (60.98%), Query Frame = 1


Query: 144 RQGAP---RSAIERLMKEKYDGNGGEEMEDECSVCYEDL-LGKTEKEKEVSRIPCGHMFH 203
           R+GAP   +SAIE L   +   + GE M   C+VC + + +G+T K     ++PCGH +H
Sbjct: 227 RRGAPPAAKSAIEALETFEVSSSEGE-MVMVCAVCKDGMVMGETGK-----KLPCGHCYH 286

Query: 204 KSCILKWLKISNSCPLCRGELE 222
             CI+ WL   NSCP+CR +LE
Sbjct: 287 GDCIVPWLGTRNSCPVCRFQLE 302

[Close]

BLAST of CSPI02G27420.1 vs. Swiss-Prot
Match: RN181_DROME (E3 ubiquitin-protein ligase RNF181 homolog OS=Drosophila melanogaster GN=CG7694 PE=2 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 3.3e-09
Identity = 33/79 (41.77%), Postives = 47/79 (59.49%), Query Frame = 1


Query: 143 VRQGAPRSAIERLMKEKYDGNGGEEMEDECSVCYEDLLGKTEKEKEVSRIPCGHMFHKSC 202
           V + + R+ +E  + E    + G ++E  CSVC E      E+ ++   +PC H FH+ C
Sbjct: 43  VPEASKRAILELPVHEIVKSDEGGDLE--CSVCKEP----AEEGQKYRILPCKHEFHEEC 102

Query: 203 ILKWLKISNSCPLCRGELE 222
           IL WLK +NSCPLCR ELE
Sbjct: 103 ILLWLKKTNSCPLCRYELE 115

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BLAST of CSPI02G27420.1 vs. TrEMBL
Match: A0A0A0LRB0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G432790 PE=4 SV=1)

HSP 1 Score: 449.1 bits (1154), Expect = 3.1e-123
Identity = 219/222 (98.65%), Postives = 220/222 (99.10%), Query Frame = 1


Query: 1   MSSPTTFSFRCSVRCNANGSAATNGQPLQFVELRLGHFSQLVLSPSHQILQQTSPILISD 60
           MSSPTTFSFRCS+RCNANGSAATNGQPLQFVELRLGHFSQLVLSPSHQILQQTSPILISD
Sbjct: 1   MSSPTTFSFRCSIRCNANGSAATNGQPLQFVELRLGHFSQLVLSPSHQILQQTSPILISD 60

Query: 61  TLIRFPLRELEDPPFISHSSLCRFLSSYNISDAVCDAIFLKISSFAFQLVAGNLNSNFHI 120
           TLIRFPLRELEDPPFISHSSLCRFLSSYNISDAVCDAIFLKISSFAFQLVAGNLNSNFHI
Sbjct: 61  TLIRFPLRELEDPPFISHSSLCRFLSSYNISDAVCDAIFLKISSFAFQLVAGNLNSNFHI 120

Query: 121 IAALDFVYTHWVDLDPAEDAAVVRQGAPRSAIERLMKEKYDGNGGEEMEDECSVCYEDLL 180
           IAALDFVYTHWVDLDPAEDAA VRQGAPRSAIERLMKEKYDGNGGEEMEDECSVCYEDL 
Sbjct: 121 IAALDFVYTHWVDLDPAEDAAAVRQGAPRSAIERLMKEKYDGNGGEEMEDECSVCYEDLH 180

Query: 181 GKTEKEKEVSRIPCGHMFHKSCILKWLKISNSCPLCRGELEA 223
           GKTEKEKEVSRIPCGHMFHKSCILKWLKISNSCPLCRGELEA
Sbjct: 181 GKTEKEKEVSRIPCGHMFHKSCILKWLKISNSCPLCRGELEA 222

[Close]

BLAST of CSPI02G27420.1 vs. TrEMBL
Match: A0A0A0L3Z2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G551140 PE=4 SV=1)

HSP 1 Score: 325.5 bits (833), Expect = 5.1e-86
Identity = 169/230 (73.48%), Postives = 184/230 (80.00%), Query Frame = 1


Query: 1   MSSPTTFSFRCSVRCNANGSAATNGQPLQFVELRLGHFSQLVLSPSHQILQQTSPILISD 60
           MS P TFSF+CS+R N   S AT+GQPLQFV+LRL HF QLV +PS QILQQTSP LISD
Sbjct: 1   MSLPITFSFQCSIRRNGKSSLATDGQPLQFVQLRLRHFGQLVHTPSQQILQQTSPTLISD 60

Query: 61  TLIRFPLRELEDPPFISHSSLCRFLSSYNISDAVCDAIFLKISSFAFQLVA---GNLNSN 120
           TLI F L ELEDPPFIS+  L RFLSSY+IS AVCDAIF  ISSFA Q++     N+N N
Sbjct: 61  TLIPFSLWELEDPPFISYFFLFRFLSSYDISAAVCDAIFYNISSFAGQILLAADSNINVN 120

Query: 121 FHIIAALDFVYTHWVDLDPAEDAAVVRQGAPRSAIERLMKEKYDGNGGEEME--DECSVC 180
           FHIIA +D VYTHWVDLDPAEDA  VR+GA  S IER+MKEKYDGNGGEE E  D+CSVC
Sbjct: 121 FHIIADVDIVYTHWVDLDPAEDAPAVRKGASTSVIERVMKEKYDGNGGEEKEGDDQCSVC 180

Query: 181 YEDLLGK----TEKEKEVSRIPCGHMFHKSCILKWLKISNSCPLCRGELE 222
           YE L GK     EKEKEVSRIPCGHM+HKSCILKWLKISNSCPLCR ELE
Sbjct: 181 YEQLYGKRENENEKEKEVSRIPCGHMYHKSCILKWLKISNSCPLCRRELE 230

[Close]

BLAST of CSPI02G27420.1 vs. TrEMBL
Match: A0A0A0LHI3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G889100 PE=4 SV=1)

HSP 1 Score: 199.5 bits (506), Expect = 4.2e-48
Identity = 119/224 (53.12%), Postives = 144/224 (64.29%), Query Frame = 1


Query: 1   MSSPTTFSFRCSVRCNANGSAATNGQPLQFVELRLGHFSQLVLSPSHQILQQTSPILISD 60
           MSS T  S  C VR     S  T     Q VE+RL  FS+LV   S+Q +QQT P  IS+
Sbjct: 1   MSSTTNLSLDCCVR-----SIPTTADK-QLVEIRLRRFSKLVSPFSNQNIQQTFPTSISN 60

Query: 61  TLIRFPLRELE-DPPFISHSSLCRFLSSYNISDAVCDAIFLKISSFAFQLVAGNLNSNFH 120
            L  FPL ELE + P + H  L +FL     S A   AIF  ISSFA QL AGNLN NFH
Sbjct: 61  ALFHFPLPELELENPSLFHDFLLQFL-----SPADSAAIFHNISSFALQLAAGNLNLNFH 120

Query: 121 IIAALDFVYTHWV--DLDPAEDAAVVRQGAPRSAIERLMKEKY-DGNGGEEMEDECSVCY 180
           +IA +D +YT  +  +L+P + +  VR G P S +ERLM EKY DGNGGE M  +C VCY
Sbjct: 121 LIATVDLLYTISINLNLNPPQYSPPVRNGVPASVMERLMNEKYDDGNGGEGMGGQCCVCY 180

Query: 181 EDLLGKTEKEK-EVSRIPCGHMFHKSCILKWLKISNSCPLCRGE 220
           EDL  + E+EK E +RIPCGH++HKSCILKWL ++NSCPLCR E
Sbjct: 181 EDLNCEREEEKEEATRIPCGHVYHKSCILKWLNVNNSCPLCRSE 213

[Close]

BLAST of CSPI02G27420.1 vs. TrEMBL
Match: A0A0A0LHS2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G130680 PE=4 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 1.6e-26
Identity = 89/236 (37.71%), Postives = 130/236 (55.08%), Query Frame = 1


Query: 6   TFSFRCSVRCNANGSAATNGQPLQFVELRLGHFSQLV---LSPS---HQILQQTSPILIS 65
           +FS    V+C+ +G   T+    Q + +RL   S+L+   L+P      IL+QT   LI 
Sbjct: 2   SFSATSCVQCSFSGYPNTD----QTLIVRLRFCSRLISQSLAPEIVPDVILEQTPFTLIG 61

Query: 66  DTL-----IRFPLRELEDPPFISHSSLCRFLSSYNISDAVCDAIFLKISSFAFQLVAGNL 125
           + L     +++PL++ E    ++      FL S+N+  +VC  +   I SFA Q    N 
Sbjct: 62  EALFSLSNLQYPLQQQELEYMVNE-----FLRSFNLRSSVCRIVSHNILSFAQQFDINN- 121

Query: 126 NSNFHIIAALDFVYTHWVDLDP-------AEDAAVVRQGAPRSAIERLMKEKYDGNGGEE 185
              F I   +D++  +W +  P         +  +  +GA  SAIER+ K+K+DG  GEE
Sbjct: 122 -ECFKIETNVDYIKEYWTEAAPELYPISTVGEEGLPPRGASESAIERVKKQKFDGFRGEE 181

Query: 186 MED--ECSVCYEDLLGKTEKEKEVSRIPCGHMFHKSCILKWLKISNSCPLCRGELE 222
            E+  +CSVC E++     K  EV RIPCGH++HKSCILKWL+ISNSCPLCR  LE
Sbjct: 182 EEEIGDCSVCCEEI---KRKGGEVRRIPCGHVYHKSCILKWLEISNSCPLCRAALE 223

[Close]

BLAST of CSPI02G27420.1 vs. TrEMBL
Match: A0A0A0K2W2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G025210 PE=4 SV=1)

HSP 1 Score: 107.1 bits (266), Expect = 2.9e-20
Identity = 74/205 (36.10%), Postives = 108/205 (52.68%), Query Frame = 1


Query: 38  FSQLVLSPS------HQILQQTSPILISDTLIRFPLRELEDPPFISHSSLCRFLSSYNIS 97
           F Q ++S S      H++L QT  I   + L    LR+L+ P F     + + LSS NI 
Sbjct: 30  FIQQIISVSPLTGRPHEVLHQTPRIPYHNALFYLSLRQLQSPLFY----ITQILSSLNIR 89

Query: 98  DAVCDAIFLKISSFAFQLVAGNLNSNFHIIAALDFVYTHW--------------VDLDPA 157
            A    +  +I+S   Q+   N  +NFHI+A +DF+   W              V LD A
Sbjct: 90  PAASQNMARRIASHILQMPDRNSETNFHILAEVDFIRLIWLPSMGGGGAGAEETVVLDEA 149

Query: 158 EDAAVVRQGAPRSAIERLMKEKYDGNGGEEMED--ECSVCYEDLLGKTEKEKEVSRIPCG 217
             A  V++G   +  ERL  E       E+ME+  +CS+C ++L   + +++EV RIPCG
Sbjct: 150 PPA--VKRGVGVARGERLRSE-------EKMEELGDCSICLDEL---SCEKREVMRIPCG 209

Query: 218 HMFHKSCILKWLKISNSCPLCRGEL 221
           H++H+SCI KWL+  NSCPLCR  L
Sbjct: 210 HVYHESCIFKWLENHNSCPLCRKPL 218

[Close]

BLAST of CSPI02G27420.1 vs. TAIR10
Match: AT4G05350.1 (AT4G05350.1 RING/U-box superfamily protein)

HSP 1 Score: 65.9 bits (159), Expect = 3.7e-11
Identity = 43/137 (31.39%), Postives = 66/137 (48.18%), Query Frame = 1


Query: 85  LSSYNISDAVCDAIFLKISSFAFQLVAGNLNSNFHIIAALDFVYTHWVDLDPAEDAAVVR 144
           L  Y     +CD +  KIS+ A  L  G+  + F +  A    Y            +V+ 
Sbjct: 80  LVGYPFESYLCDDLAPKISTAAIDLGFGH--NGFTLTMAATVTY---------RTVSVMS 139

Query: 145 QGAPRSAIERLMKEKYDGNGGEEMEDE-CSVCYEDLLGKTEKEKEVSRIPCGHMFHKSCI 204
                  +  L + K +     +ME E CS+C E L+    K ++V+R+ C H+FH  C+
Sbjct: 140 NDEKSLRMVLLGRMKAEEFKSLKMETEPCSICLESLVSGP-KPRDVTRMTCSHVFHNGCL 199

Query: 205 LKWLKISNSCPLCRGEL 221
           L+WLK  N+CPLCR E+
Sbjct: 200 LEWLKRKNTCPLCRTEI 204

[Close]

BLAST of CSPI02G27420.1 vs. TAIR10
Match: AT4G12190.1 (AT4G12190.1 RING/U-box superfamily protein)

HSP 1 Score: 64.7 bits (156), Expect = 8.3e-11
Identity = 27/55 (49.09%), Postives = 39/55 (70.91%), Query Frame = 1


Query: 167 EMEDE-CSVCYEDLLGKTEKEKEVSRIPCGHMFHKSCILKWLKISNSCPLCRGEL 221
           +ME E CS+C E L+    K ++++R+ C H+FH  C+L+WLK  N+CPLCR EL
Sbjct: 16  KMETEPCSICLESLVSGP-KPRDITRMTCSHVFHNGCLLEWLKRKNTCPLCRTEL 69

[Close]

BLAST of CSPI02G27420.1 vs. TAIR10
Match: AT5G56340.1 (AT5G56340.1 RING/U-box superfamily protein)

HSP 1 Score: 63.9 bits (154), Expect = 1.4e-10
Identity = 40/108 (37.04%), Postives = 54/108 (50.00%), Query Frame = 1


Query: 117 NFHIIAALDFVYTHWVDLDPAEDAAVVRQGAP---RSAIERLMKEKYDGNGGEEMED-EC 176
           ++ I   LD +  H  + DP       RQG P   + A+E L   K        ME  +C
Sbjct: 213 DYFIGPGLDLLLQHLAENDPN------RQGTPPARKEAVEALPTVKI-------MEPLQC 272

Query: 177 SVCYEDLLGKTEKEKEVSRIPCGHMFHKSCILKWLKISNSCPLCRGEL 221
           SVC +D     EK  E   +PC H FH  CI+ WL++ +SCP+CR EL
Sbjct: 273 SVCLDDF----EKGTEAKEMPCKHKFHVRCIVPWLELHSSCPVCRFEL 303

[Close]

BLAST of CSPI02G27420.1 vs. TAIR10
Match: AT5G37230.1 (AT5G37230.1 RING/U-box superfamily protein)

HSP 1 Score: 63.5 bits (153), Expect = 1.8e-10
Identity = 45/145 (31.03%), Postives = 62/145 (42.76%), Query Frame = 1


Query: 75  FISHSSLCRFLSSYNISDAVCDAIFLKISSFAFQLVAGNLNSNFHIIAALDFVYTHWVDL 134
           FI+H+  CR LS   + D                          HI       +   V  
Sbjct: 81  FITHTEFCRLLSERIVLDCA------------------------HIPQPFSVSFYVRVTR 140

Query: 135 DPAEDAAVVRQGAPRSAIERLMKEKYDG--NGGEEMEDECSVCYEDLLGKTEKEKEVSRI 194
           D    + VVR    R   +RL++E+     N G+E E  CS+C ED   ++  +  +   
Sbjct: 141 DVMLPSIVVRS---RDMFQRLLEEQTMELTNLGDEEETTCSICMEDF-SESHDDNIILLP 197

Query: 195 PCGHMFHKSCILKWLKISNSCPLCR 218
            C H+FH+SCI KWLK   SCPLCR
Sbjct: 201 DCFHLFHQSCIFKWLKRQRSCPLCR 197

[Close]

BLAST of CSPI02G27420.1 vs. TAIR10
Match: AT5G64920.1 (AT5G64920.1 COP1-interacting protein 8)

HSP 1 Score: 63.5 bits (153), Expect = 1.8e-10
Identity = 35/82 (42.68%), Postives = 50/82 (60.98%), Query Frame = 1


Query: 144 RQGAP---RSAIERLMKEKYDGNGGEEMEDECSVCYEDL-LGKTEKEKEVSRIPCGHMFH 203
           R+GAP   +SAIE L   +   + GE M   C+VC + + +G+T K     ++PCGH +H
Sbjct: 227 RRGAPPAAKSAIEALETFEVSSSEGE-MVMVCAVCKDGMVMGETGK-----KLPCGHCYH 286

Query: 204 KSCILKWLKISNSCPLCRGELE 222
             CI+ WL   NSCP+CR +LE
Sbjct: 287 GDCIVPWLGTRNSCPVCRFQLE 302

[Close]

BLAST of CSPI02G27420.1 vs. NCBI nr
Match: gi|778674460|ref|XP_011650219.1| (PREDICTED: RING-H2 finger protein ATL16-like [Cucumis sativus])

HSP 1 Score: 449.1 bits (1154), Expect = 4.4e-123
Identity = 219/222 (98.65%), Postives = 220/222 (99.10%), Query Frame = 1


Query: 1   MSSPTTFSFRCSVRCNANGSAATNGQPLQFVELRLGHFSQLVLSPSHQILQQTSPILISD 60
           MSSPTTFSFRCS+RCNANGSAATNGQPLQFVELRLGHFSQLVLSPSHQILQQTSPILISD
Sbjct: 1   MSSPTTFSFRCSIRCNANGSAATNGQPLQFVELRLGHFSQLVLSPSHQILQQTSPILISD 60

Query: 61  TLIRFPLRELEDPPFISHSSLCRFLSSYNISDAVCDAIFLKISSFAFQLVAGNLNSNFHI 120
           TLIRFPLRELEDPPFISHSSLCRFLSSYNISDAVCDAIFLKISSFAFQLVAGNLNSNFHI
Sbjct: 61  TLIRFPLRELEDPPFISHSSLCRFLSSYNISDAVCDAIFLKISSFAFQLVAGNLNSNFHI 120

Query: 121 IAALDFVYTHWVDLDPAEDAAVVRQGAPRSAIERLMKEKYDGNGGEEMEDECSVCYEDLL 180
           IAALDFVYTHWVDLDPAEDAA VRQGAPRSAIERLMKEKYDGNGGEEMEDECSVCYEDL 
Sbjct: 121 IAALDFVYTHWVDLDPAEDAAAVRQGAPRSAIERLMKEKYDGNGGEEMEDECSVCYEDLH 180

Query: 181 GKTEKEKEVSRIPCGHMFHKSCILKWLKISNSCPLCRGELEA 223
           GKTEKEKEVSRIPCGHMFHKSCILKWLKISNSCPLCRGELEA
Sbjct: 181 GKTEKEKEVSRIPCGHMFHKSCILKWLKISNSCPLCRGELEA 222

[Close]

BLAST of CSPI02G27420.1 vs. NCBI nr
Match: gi|700199693|gb|KGN54851.1| (hypothetical protein Csa_4G551140 [Cucumis sativus])

HSP 1 Score: 325.5 bits (833), Expect = 7.4e-86
Identity = 169/230 (73.48%), Postives = 184/230 (80.00%), Query Frame = 1


Query: 1   MSSPTTFSFRCSVRCNANGSAATNGQPLQFVELRLGHFSQLVLSPSHQILQQTSPILISD 60
           MS P TFSF+CS+R N   S AT+GQPLQFV+LRL HF QLV +PS QILQQTSP LISD
Sbjct: 1   MSLPITFSFQCSIRRNGKSSLATDGQPLQFVQLRLRHFGQLVHTPSQQILQQTSPTLISD 60

Query: 61  TLIRFPLRELEDPPFISHSSLCRFLSSYNISDAVCDAIFLKISSFAFQLVA---GNLNSN 120
           TLI F L ELEDPPFIS+  L RFLSSY+IS AVCDAIF  ISSFA Q++     N+N N
Sbjct: 61  TLIPFSLWELEDPPFISYFFLFRFLSSYDISAAVCDAIFYNISSFAGQILLAADSNINVN 120

Query: 121 FHIIAALDFVYTHWVDLDPAEDAAVVRQGAPRSAIERLMKEKYDGNGGEEME--DECSVC 180
           FHIIA +D VYTHWVDLDPAEDA  VR+GA  S IER+MKEKYDGNGGEE E  D+CSVC
Sbjct: 121 FHIIADVDIVYTHWVDLDPAEDAPAVRKGASTSVIERVMKEKYDGNGGEEKEGDDQCSVC 180

Query: 181 YEDLLGK----TEKEKEVSRIPCGHMFHKSCILKWLKISNSCPLCRGELE 222
           YE L GK     EKEKEVSRIPCGHM+HKSCILKWLKISNSCPLCR ELE
Sbjct: 181 YEQLYGKRENENEKEKEVSRIPCGHMYHKSCILKWLKISNSCPLCRRELE 230

[Close]

BLAST of CSPI02G27420.1 vs. NCBI nr
Match: gi|659132530|ref|XP_008466247.1| (PREDICTED: uncharacterized protein LOC103503717 [Cucumis melo])

HSP 1 Score: 221.1 bits (562), Expect = 2.0e-54
Identity = 129/226 (57.08%), Postives = 151/226 (66.81%), Query Frame = 1


Query: 1   MSSPTTFSFRCSVRCNANGSAATNGQPLQFVELRLGHFSQLVLSPSHQILQQTSPILISD 60
           MSS TT S  C +    + S AT     Q VE+RL  +S+LV   S+QI+QQT P  ISD
Sbjct: 1   MSSTTTLSLECCI----HSSVATTADE-QLVEIRLRRYSKLVSPSSNQIIQQTFPTPISD 60

Query: 61  TLIRFPLRELE--DPPFISHSSLCRFLSSYNISDAVCDAIFLKISSFAFQLVAGNLNSNF 120
            L  FPL ELE  +PPF  H  L +FLS      A   AIF  ISSFA QL AGNLN NF
Sbjct: 61  ALFHFPLPELELENPPFFFHDLLLQFLSP-----ADSTAIFHNISSFALQLAAGNLNLNF 120

Query: 121 HIIAALDFVYTHWVDLD--PAEDAAVVRQGAPRSAIERLMKEKYD-GNGGEEMEDECSVC 180
           H+IA +D +YT  VDLD  P + + +VR GAP S IERLM EKYD GNG EEM D+C VC
Sbjct: 121 HLIATVDLLYTISVDLDLDPPQYSPLVRNGAPASVIERLMDEKYDDGNGREEMGDQCCVC 180

Query: 181 YEDLL--GKTEKEKEVSRIPCGHMFHKSCILKWLKISNSCPLCRGE 220
           YEDL    + EKE+EV+RIPCGH++HK CILKWL I+NSCPLCR E
Sbjct: 181 YEDLNCEREEEKEEEVTRIPCGHVYHKYCILKWLNINNSCPLCRRE 216

[Close]

BLAST of CSPI02G27420.1 vs. NCBI nr
Match: gi|778689036|ref|XP_011652885.1| (PREDICTED: RING-H2 finger protein ATL66-like [Cucumis sativus])

HSP 1 Score: 199.5 bits (506), Expect = 6.1e-48
Identity = 119/224 (53.12%), Postives = 144/224 (64.29%), Query Frame = 1


Query: 1   MSSPTTFSFRCSVRCNANGSAATNGQPLQFVELRLGHFSQLVLSPSHQILQQTSPILISD 60
           MSS T  S  C VR     S  T     Q VE+RL  FS+LV   S+Q +QQT P  IS+
Sbjct: 1   MSSTTNLSLDCCVR-----SIPTTADK-QLVEIRLRRFSKLVSPFSNQNIQQTFPTSISN 60

Query: 61  TLIRFPLRELE-DPPFISHSSLCRFLSSYNISDAVCDAIFLKISSFAFQLVAGNLNSNFH 120
            L  FPL ELE + P + H  L +FL     S A   AIF  ISSFA QL AGNLN NFH
Sbjct: 61  ALFHFPLPELELENPSLFHDFLLQFL-----SPADSAAIFHNISSFALQLAAGNLNLNFH 120

Query: 121 IIAALDFVYTHWV--DLDPAEDAAVVRQGAPRSAIERLMKEKY-DGNGGEEMEDECSVCY 180
           +IA +D +YT  +  +L+P + +  VR G P S +ERLM EKY DGNGGE M  +C VCY
Sbjct: 121 LIATVDLLYTISINLNLNPPQYSPPVRNGVPASVMERLMNEKYDDGNGGEGMGGQCCVCY 180

Query: 181 EDLLGKTEKEK-EVSRIPCGHMFHKSCILKWLKISNSCPLCRGE 220
           EDL  + E+EK E +RIPCGH++HKSCILKWL ++NSCPLCR E
Sbjct: 181 EDLNCEREEEKEEATRIPCGHVYHKSCILKWLNVNNSCPLCRSE 213

[Close]

BLAST of CSPI02G27420.1 vs. NCBI nr
Match: gi|659121671|ref|XP_008460768.1| (PREDICTED: E3 ubiquitin-protein ligase RING1-like [Cucumis melo])

HSP 1 Score: 130.6 bits (327), Expect = 3.5e-27
Identity = 91/225 (40.44%), Postives = 123/225 (54.67%), Query Frame = 1


Query: 1   MSSPTTFSFRCSVRCNANGSAATNGQPLQFVELRLGHFSQLVLSPSHQILQQTSPILISD 60
           MS  T+ SF+CS     N           F+  R G+ S+L++    QILQQT  IL+ +
Sbjct: 1   MSLSTSSSFQCSFSATPNTD--------HFLICRFGYSSRLIIVD--QILQQTPSILMGE 60

Query: 61  TLIRFPLRELEDPPFISHSSLCRFLSSYNISD--AVCDAIFLKISSFAFQLVAGNLNSNF 120
           TLI F      +        +  FL S+N+    +    I  KI SFA Q    N++ +F
Sbjct: 61  TLIPFSNLWHPEQQQSIEFLVNEFLGSFNLHSFASARRTICRKILSFALQF--HNVHGSF 120

Query: 121 HIIAALDFVYTHWVDLDPAEDAAVVRQ--GAPRSAIERLMKEKYDGNGGEEMEDECSVCY 180
            I A ++ V    +     ++     +  GAP SAIERL KEK+D    EE   +CSVCY
Sbjct: 121 KIEANVEHVEDLIIGEQGFQETPSPPRTTGAPESAIERLRKEKFDVLNKEEEIGDCSVCY 180

Query: 181 EDLLGKTEKEKEVSRIPCGHMFHKSCILKWLKISNSCPLCRGELE 222
           E+L G+    KEVSRIPCGH++HKSCIL WL+I+NSCPLCR  L+
Sbjct: 181 EELKGQG---KEVSRIPCGHVYHKSCILTWLQINNSCPLCRAPLQ 210

The following BLAST results are available for this feature:

BLAST of CSPI02G27420.1 vs. Swiss-Prot


Match Name	E-value	Identity	Description
RING1_GOSHI	1.9e-09	39.19	E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum GN=RING1 PE=1 SV=1	[more]
YGI4_SCHPO	2.5e-09	36.99	Uncharacterized RING finger protein C2A9.04c OS=Schizosaccharomyces pombe (strai...	[more]
RN111_PONAB	3.3e-09	34.09	E3 ubiquitin-protein ligase Arkadia OS=Pongo abelii GN=RNF111 PE=2 SV=1	[more]
CIP8_ARATH	3.3e-09	42.68	E3 ubiquitin-protein ligase CIP8 OS=Arabidopsis thaliana GN=CIP8 PE=1 SV=1	[more]
RN181_DROME	3.3e-09	41.77	E3 ubiquitin-protein ligase RNF181 homolog OS=Drosophila melanogaster GN=CG7694 ...	[more]

BLAST of CSPI02G27420.1 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LRB0_CUCSA	3.1e-123	98.65	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G432790 PE=4 SV=1	[more]
A0A0A0L3Z2_CUCSA	5.1e-86	73.48	Uncharacterized protein OS=Cucumis sativus GN=Csa_4G551140 PE=4 SV=1	[more]
A0A0A0LHI3_CUCSA	4.2e-48	53.13	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G889100 PE=4 SV=1	[more]
A0A0A0LHS2_CUCSA	1.6e-26	37.71	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G130680 PE=4 SV=1	[more]
A0A0A0K2W2_CUCSA	2.9e-20	36.10	Uncharacterized protein OS=Cucumis sativus GN=Csa_7G025210 PE=4 SV=1	[more]

BLAST of CSPI02G27420.1 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G05350.1	3.7e-11	31.39	RING/U-box superfamily protein	[more]
AT4G12190.1	8.3e-11	49.09	RING/U-box superfamily protein	[more]
AT5G56340.1	1.4e-10	37.04	RING/U-box superfamily protein	[more]
AT5G37230.1	1.8e-10	31.03	RING/U-box superfamily protein	[more]
AT5G64920.1	1.8e-10	42.68	COP1-interacting protein 8	[more]

BLAST of CSPI02G27420.1 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|778674460\|ref\|XP_011650219.1\|	4.4e-123	98.65	PREDICTED: RING-H2 finger protein ATL16-like [Cucumis sativus]	[more]
gi\|700199693\|gb\|KGN54851.1\|	7.4e-86	73.48	hypothetical protein Csa_4G551140 [Cucumis sativus]	[more]
gi\|659132530\|ref\|XP_008466247.1\|	2.0e-54	57.08	PREDICTED: uncharacterized protein LOC103503717 [Cucumis melo]	[more]
gi\|778689036\|ref\|XP_011652885.1\|	6.1e-48	53.13	PREDICTED: RING-H2 finger protein ATL66-like [Cucumis sativus]	[more]
gi\|659121671\|ref\|XP_008460768.1\|	3.5e-27	40.44	PREDICTED: E3 ubiquitin-protein ligase RING1-like [Cucumis melo]	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **INTERPRO**
Term	Definition
IPR001841	Znf_RING
IPR013083	Znf_RING/FYVE/PHD

Vocabulary: **Molecular Function**
Term	Definition
GO:0005515	protein binding
GO:0008270	zinc ion binding

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0005515	protein binding
molecular_function	GO:0008270	zinc ion binding

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CSPI02G27420	CSPI02G27420	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CSPI02G27420.1	CSPI02G27420.1-protein	polypeptide

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI02G27420.1.cds1	CSPI02G27420.1.cds1	CDS

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI02G27420.1.utr5p1	CSPI02G27420.1.utr5p1	five_prime_UTR

InterPro

Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 170..217 score: 3.3
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 172..216 score: 1.
IPR001841	Zinc finger, RING-type	PROFILE	PS50089	ZF_RING_2	coord: 172..217 score: 13
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	G3DSA:3.30.40.10		coord: 168..220 score: 1.2
None	No IPR available	PANTHER	PTHR22763	RING ZINC FINGER PROTEIN	coord: 145..221 score: 1.1
None	No IPR available	unknown	SSF57850	RING/U-box	coord: 166..220 score: 1.15