CSPI01G25140 (gene) Wild cucumber (PI 183967)

BLAST of CSPI01G25140 vs. Swiss-Prot
Match: THCAS_CANSA (Tetrahydrocannabinolic acid synthase OS=Cannabis sativa PE=1 SV=1)

HSP 1 Score: 531.6 bits (1368), Expect = 1.0e-149
Identity = 261/512 (50.98%), Postives = 348/512 (67.97%), Query Frame = 1

Query: 33  QNFLHCLSEHSSKSYPISKVVYTPINSSYSSVLDFSIRNLLFSKPETPKPLLIITPSHIS 92
           +NFL C S+H   +    K+VYT  +  Y S+L+ +I+NL F    TPKPL+I+TPS+ S
Sbjct: 32  ENFLKCFSKHIPNNVANPKLVYTQHDQLYMSILNSTIQNLRFISDTTPKPLVIVTPSNNS 91

Query: 93  HIQAAVICSKSHGLQIRTRSGGHDFEGLSYVAYRPFIVVDLINLRSVKVDVENNTAWVES 152
           HIQA ++CSK  GLQIRTRSGGHD EG+SY++  PF+VVDL N+ S+K+DV + TAWVE+
Sbjct: 92  HIQATILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVVVDLRNMHSIKIDVHSQTAWVEA 151

Query: 153 GATLGELYYKIGEKSRTLAFPAGVCPTVGIGGHFSGGGYGLMLRKFGLAADNVIDAYLVD 212
           GATLGE+YY I EK+  L+FP G CPTVG+GGHFSGGGYG ++R +GLAADN+IDA+LV+
Sbjct: 152 GATLGEVYYWINEKNENLSFPGGYCPTVGVGGHFSGGGYGALMRNYGLAADNIIDAHLVN 211

Query: 213 AHGKVLDRESMGEDLFWAIRGGGGGSFGIVVAWKIKLVRVPPTVTICSTDRNLE-EDTIR 272
             GKVLDR+SMGEDLFWAIRGGGG +FGI+ AWKIKLV VP   TI S  +N+E    ++
Sbjct: 212 VDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIKLVAVPSKSTIFSVKKNMEIHGLVK 271

Query: 273 LIHRWQYVGNKLDENVYLGIILTGGNASTQAGITNPTAR--FFSLFLGRVDEFMATLSTT 332
           L ++WQ +  K D+++ L       N +   G    T    F S+F G VD  +  ++ +
Sbjct: 272 LFNKWQNIAYKYDKDLVLMTHFITKNITDNHGKNKTTVHGYFSSIFHGGVDSLVDLMNKS 331

Query: 333 FPELGLIKQDCVEASWVESTLIIPIGVQPIESL----EPLLNRTPTYLDSTKIKSDYVKE 392
           FPELG+ K DC E SW+++T+    GV    +     E LL+R+     +  IK DYVK+
Sbjct: 332 FPELGIKKTDCKEFSWIDTTIFYS-GVVNFNTANFKKEILLDRSAGKKTAFSIKLDYVKK 391

Query: 393 PISEATIEGIWQRLKAQDIETSQVIFVPYGGRMSQISESETPFPHRAGYLFKIAYVVGWK 452
           PI E  +  I ++L  +D+     +  PYGG M +ISES  PFPHRAG ++++ Y   W+
Sbjct: 392 PIPETAMVKILEKLYEEDVGAGMYVLYPYGGIMEEISESAIPFPHRAGIMYELWYTASWE 451

Query: 453 DQSLKAKKTHISWIREIYEYMAPFVSKSPRAAYTNYRDLDIGSNNKYGKTSYKRASIWGM 512
            Q    K  HI+W+R +Y +  P+VS++PR AY NYRDLD+G  N     +Y +A IWG 
Sbjct: 452 KQEDNEK--HINWVRSVYNFTTPYVSQNPRLAYLNYRDLDLGKTNHASPNNYTQARIWGE 511

Query: 513 KYFGDNFDRLVYVKTKVDPYDFFRHEQSIPTL 538
           KYFG NF+RLV VKTKVDP +FFR+EQSIP L
Sbjct: 512 KYFGKNFNRLVKVKTKVDPNNFFRNEQSIPPL 540

BLAST of CSPI01G25140 vs. Swiss-Prot
Match: CASL1_CANSA (Cannabidiolic acid synthase-like 1 OS=Cannabis sativa GN=CBDAS2 PE=2 SV=1)

HSP 1 Score: 528.1 bits (1359), Expect = 1.1e-148
Identity = 262/518 (50.58%), Postives = 355/518 (68.53%), Query Frame = 1

Query: 27  ASPQKYQNFLHCLSEHSSKSYPISKVVYTPINSSYSSVLDFSIRNLLFSKPETPKPLLII 86
           A+PQ  +NFL C S++   +   +K+VYT  +  Y S+L+ +I+NL F+   TPKPL+II
Sbjct: 28  ANPQ--ENFLKCFSQYIPTNVTNAKLVYTQHDQFYMSILNSTIQNLRFTSDTTPKPLVII 87

Query: 87  TPSHISHIQAAVICSKSHGLQIRTRSGGHDFEGLSYVAYRPFIVVDLINLRSVKVDVENN 146
           TP ++SHIQ  ++CSK  GLQIRTRSGGHD EG+SY++  PF++VDL N+ SVK+DV + 
Sbjct: 88  TPLNVSHIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLRNMHSVKIDVHSQ 147

Query: 147 TAWVESGATLGELYYKIGEKSRTLAFPAGVCPTVGIGGHFSGGGYGLMLRKFGLAADNVI 206
           TAWVE+GATLGE+YY I E +  L+FPAG CPTVG GGHFSGGGYG ++R +GLAADN+I
Sbjct: 148 TAWVEAGATLGEVYYWINENNENLSFPAGYCPTVGAGGHFSGGGYGALMRNYGLAADNII 207

Query: 207 DAYLVDAHGKVLDRESMGEDLFWAIRGGGGGSFGIVVAWKIKLVRVPPTVTICSTDRNLE 266
           DA+LV+  GKVLDR+SMGEDLFWAIRGGGG +FGI+ AWKI+LV VP   TI S  +N+E
Sbjct: 208 DAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVPSMSTIFSVKKNME 267

Query: 267 -EDTIRLIHRWQYVGNKLDENVYLGIILTGGNASTQAGITNPT--ARFFSLFLGRVDEFM 326
             + ++L+++WQ +    ++ + L       N +   G    T  + F S+F G VD  +
Sbjct: 268 IHELVKLVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTTIHSYFSSIFHGGVDSLV 327

Query: 327 ATLSTTFPELGLIKQDCVEASWVESTLIIPIGVQPIESL----EPLLNRTPTYLDSTKIK 386
             ++ +FPELG+ K DC + SW++ T+I   GV    +     E LL+R+     +  IK
Sbjct: 328 DLMNKSFPELGIKKTDCKQLSWID-TIIFYSGVVNYNTTYFKKEILLDRSGGRKAAFSIK 387

Query: 387 SDYVKEPISEATIEGIWQRLKAQDIETSQVIFVPYGGRMSQISESETPFPHRAGYLFKIA 446
            DYVK+PI E  +  I ++L  +D+     +F PYGG M +ISES  PFPHRAG +++I 
Sbjct: 388 LDYVKKPIPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISESAIPFPHRAGIMYEIW 447

Query: 447 YVVGWKDQSLKAKKTHISWIREIYEYMAPFVSKSPRAAYTNYRDLDIGSNNKYGKTSYKR 506
           Y+  W+ Q    K  HI+WIR +Y +  P+VS++PR AY NYRDLD+G  N     +Y +
Sbjct: 448 YIASWEKQEDNEK--HINWIRNVYNFTTPYVSQNPRMAYLNYRDLDLGKTNFESPNNYTQ 507

Query: 507 ASIWGMKYFGDNFDRLVYVKTKVDPYDFFRHEQSIPTL 538
           A IWG KYFG NF+RLV VKTKVDP +FFR+EQSIP L
Sbjct: 508 ARIWGEKYFGKNFNRLVKVKTKVDPDNFFRNEQSIPPL 540

BLAST of CSPI01G25140 vs. Swiss-Prot
Match: CBDAS_CANSA (Cannabidiolic acid synthase OS=Cannabis sativa GN=CBDAS PE=1 SV=1)

HSP 1 Score: 527.7 bits (1358), Expect = 1.5e-148
Identity = 266/513 (51.85%), Postives = 354/513 (69.01%), Query Frame = 1

Query: 33  QNFLHCLSEHSSKSYPISKVVYTPINSSYSSVLDFSIRNLLFSKPETPKPLLIITPSHIS 92
           +NFL C S++   +    K+VYT  N  Y SVL+ +I NL F+   TPKPL+I+TPSH+S
Sbjct: 32  ENFLKCFSQYIPNNATNLKLVYTQNNPLYMSVLNSTIHNLRFTSDTTPKPLVIVTPSHVS 91

Query: 93  HIQAAVICSKSHGLQIRTRSGGHDFEGLSYVAYRPFIVVDLINLRSVKVDVENNTAWVES 152
           HIQ  ++CSK  GLQIRTRSGGHD EG+SY++  PF++VDL N+RS+K+DV + TAWVE+
Sbjct: 92  HIQGTILCSKKVGLQIRTRSGGHDSEGMSYISQVPFVIVDLRNMRSIKIDVHSQTAWVEA 151

Query: 153 GATLGELYYKIGEKSRTLAFPAGVCPTVGIGGHFSGGGYGLMLRKFGLAADNVIDAYLVD 212
           GATLGE+YY + EK+  L+  AG CPTV  GGHF GGGYG ++R +GLAADN+IDA+LV+
Sbjct: 152 GATLGEVYYWVNEKNENLSLAAGYCPTVCAGGHFGGGGYGPLMRNYGLAADNIIDAHLVN 211

Query: 213 AHGKVLDRESMGEDLFWAIRGGGGGSFGIVVAWKIKLVRVPPTVTICSTDRNLE-EDTIR 272
            HGKVLDR+SMGEDLFWA+RGGG  SFGI+VAWKI+LV VP + T+ S  + +E  + ++
Sbjct: 212 VHGKVLDRKSMGEDLFWALRGGGAESFGIIVAWKIRLVAVPKS-TMFSVKKIMEIHELVK 271

Query: 273 LIHRWQYVGNKLDENVYLGIILTGGNASTQAGITNPTA---RFFSLFLGRVDEFMATLST 332
           L+++WQ +  K D+++ L       N +   G  N TA    F S+FLG VD  +  ++ 
Sbjct: 272 LVNKWQNIAYKYDKDLLLMTHFITRNITDNQG-KNKTAIHTYFSSVFLGGVDSLVDLMNK 331

Query: 333 TFPELGLIKQDCVEASWVESTLIIPIGVQPIE----SLEPLLNRTPTYLDSTKIKSDYVK 392
           +FPELG+ K DC + SW++ T+I   GV   +    + E LL+R+     + KIK DYVK
Sbjct: 332 SFPELGIKKTDCRQLSWID-TIIFYSGVVNYDTDNFNKEILLDRSAGQNGAFKIKLDYVK 391

Query: 393 EPISEATIEGIWQRLKAQDIETSQVIFVPYGGRMSQISESETPFPHRAGYLFKIAYVVGW 452
           +PI E+    I ++L  +DI        PYGG M +ISES  PFPHRAG L+++ Y+  W
Sbjct: 392 KPIPESVFVQILEKLYEEDIGAGMYALYPYGGIMDEISESAIPFPHRAGILYELWYICSW 451

Query: 453 KDQSLKAKKTHISWIREIYEYMAPFVSKSPRAAYTNYRDLDIGSNNKYGKTSYKRASIWG 512
           + Q    K  H++WIR IY +M P+VSK+PR AY NYRDLDIG N+     +Y +A IWG
Sbjct: 452 EKQEDNEK--HLNWIRNIYNFMTPYVSKNPRLAYLNYRDLDIGINDPKNPNNYTQARIWG 511

Query: 513 MKYFGDNFDRLVYVKTKVDPYDFFRHEQSIPTL 538
            KYFG NFDRLV VKT VDP +FFR+EQSIP L
Sbjct: 512 EKYFGKNFDRLVKVKTLVDPNNFFRNEQSIPPL 539

BLAST of CSPI01G25140 vs. Swiss-Prot
Match: CASL2_CANSA (Cannabidiolic acid synthase-like 2 OS=Cannabis sativa GN=CBDAS3 PE=2 SV=1)

HSP 1 Score: 527.3 bits (1357), Expect = 1.9e-148
Identity = 262/518 (50.58%), Postives = 354/518 (68.34%), Query Frame = 1

Query: 27  ASPQKYQNFLHCLSEHSSKSYPISKVVYTPINSSYSSVLDFSIRNLLFSKPETPKPLLII 86
           A+PQ  +NFL CLS++   +   +K+VYT  +  Y S+L+ +++NL F+   TPKPL+I 
Sbjct: 28  ANPQ--ENFLKCLSQYIPTNVTNAKLVYTQHDQFYMSILNSTVQNLRFTSDTTPKPLVIT 87

Query: 87  TPSHISHIQAAVICSKSHGLQIRTRSGGHDFEGLSYVAYRPFIVVDLINLRSVKVDVENN 146
           TP ++SHIQ  ++CSK  GLQIRTRSGGHD EG+SY++  PF++VDL N+ SVK+DV + 
Sbjct: 88  TPLNVSHIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLRNMHSVKIDVHSQ 147

Query: 147 TAWVESGATLGELYYKIGEKSRTLAFPAGVCPTVGIGGHFSGGGYGLMLRKFGLAADNVI 206
           TAWVESGATLGE+YY I E +  L+FPAG CPTVG GGHFSGGGYG ++R +GLAADN+I
Sbjct: 148 TAWVESGATLGEVYYWINENNENLSFPAGYCPTVGTGGHFSGGGYGALMRNYGLAADNII 207

Query: 207 DAYLVDAHGKVLDRESMGEDLFWAIRGGGGGSFGIVVAWKIKLVRVPPTVTICSTDRNLE 266
           DA+LV+  GKVLDR+SMGEDLFWAIRGGGG +FGI+ AWKI+LV VP   TI S  +N+E
Sbjct: 208 DAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVPSMSTIFSVKKNME 267

Query: 267 -EDTIRLIHRWQYVGNKLDENVYLGIILTGGNASTQAGITNPT--ARFFSLFLGRVDEFM 326
             + ++L+++WQ +    ++ + L       N +   G    T  + F S+F G VD  +
Sbjct: 268 IHELVKLVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTTIHSYFSSIFHGGVDSLV 327

Query: 327 ATLSTTFPELGLIKQDCVEASWVESTLIIPIGVQPIESL----EPLLNRTPTYLDSTKIK 386
             ++ +FPELG+ K DC + SW++ T+I   GV    +     E LL+R+     +  IK
Sbjct: 328 DLMNKSFPELGIKKTDCKQLSWID-TIIFYSGVVNYNTTNFKKEILLDRSGGRKAAFSIK 387

Query: 387 SDYVKEPISEATIEGIWQRLKAQDIETSQVIFVPYGGRMSQISESETPFPHRAGYLFKIA 446
            DYVK+PI E  +  I ++L  +D+     +F PYGG M +ISES  PFPHRAG  ++I 
Sbjct: 388 LDYVKKPIPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISESAIPFPHRAGITYEIW 447

Query: 447 YVVGWKDQSLKAKKTHISWIREIYEYMAPFVSKSPRAAYTNYRDLDIGSNNKYGKTSYKR 506
           Y+  W+ Q    K  HI+WIR +Y +  P+VS++PR AY NYRDLD+G  N     +Y +
Sbjct: 448 YIASWEKQEDNEK--HINWIRNVYNFTTPYVSQNPRMAYLNYRDLDLGKTNFESPNNYTQ 507

Query: 507 ASIWGMKYFGDNFDRLVYVKTKVDPYDFFRHEQSIPTL 538
           A IWG KYFG NF+RLV VKTKVDP +FFR+EQSIP L
Sbjct: 508 ARIWGEKYFGKNFNRLVKVKTKVDPDNFFRNEQSIPPL 540

BLAST of CSPI01G25140 vs. Swiss-Prot
Match: THCAI_CANSA (Inactive tetrahydrocannabinolic acid synthase OS=Cannabis sativa PE=3 SV=1)

HSP 1 Score: 524.2 bits (1349), Expect = 1.6e-147
Identity = 260/518 (50.19%), Postives = 350/518 (67.57%), Query Frame = 1

Query: 27  ASPQKYQNFLHCLSEHSSKSYPISKVVYTPINSSYSSVLDFSIRNLLFSKPETPKPLLII 86
           A+PQ  +NFL C SE+   +    K +YT  +  Y SVL+ +I+NL F+   TPKPL+I+
Sbjct: 28  ANPQ--ENFLKCFSEYIPNNPANPKFIYTQHDQLYMSVLNSTIQNLRFTSDTTPKPLVIV 87

Query: 87  TPSHISHIQAAVICSKSHGLQIRTRSGGHDFEGLSYVAYRPFIVVDLINLRSVKVDVENN 146
           TPS++SHIQA+++CSK  GLQIRTRSGGHD EGLSY++  PF +VDL N+ +VKVD+ + 
Sbjct: 88  TPSNVSHIQASILCSKKVGLQIRTRSGGHDAEGLSYISQVPFAIVDLRNMHTVKVDIHSQ 147

Query: 147 TAWVESGATLGELYYKIGEKSRTLAFPAGVCPTVGIGGHFSGGGYGLMLRKFGLAADNVI 206
           TAWVE+GATLGE+YY I E +   +FP G CPTVG+GGHFSGGGYG ++R +GLAADN+I
Sbjct: 148 TAWVEAGATLGEVYYWINEMNENFSFPGGYCPTVGVGGHFSGGGYGALMRNYGLAADNII 207

Query: 207 DAYLVDAHGKVLDRESMGEDLFWAIRGGGGGSFGIVVAWKIKLVRVPPTVTICSTDRNLE 266
           DA+LV+  GKVLDR+SMGEDLFWAIRGGGG +FGI+ AWKIKLV VP   TI S  +N+E
Sbjct: 208 DAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIKLVVVPSKATIFSVKKNME 267

Query: 267 -EDTIRLIHRWQYVGNKLDENVYLGIILTGGNASTQAGITNPTAR--FFSLFLGRVDEFM 326
               ++L ++WQ +  K D+++ L       N +   G    T    F S+FLG VD  +
Sbjct: 268 IHGLVKLFNKWQNIAYKYDKDLMLTTHFRTRNITDNHGKNKTTVHGYFSSIFLGGVDSLV 327

Query: 327 ATLSTTFPELGLIKQDCVEASWVESTLIIPIGVQPIESL----EPLLNRTPTYLDSTKIK 386
             ++ +FPELG+ K DC E SW+++T+    GV    +     E LL+R+     +  IK
Sbjct: 328 DLMNKSFPELGIKKTDCKELSWIDTTIFYS-GVVNYNTANFKKEILLDRSAGKKTAFSIK 387

Query: 387 SDYVKEPISEATIEGIWQRLKAQDIETSQVIFVPYGGRMSQISESETPFPHRAGYLFKIA 446
            DYVK+ I E  +  I ++L  +++     +  PYGG M +ISES  PFPHRAG ++++ 
Sbjct: 388 LDYVKKLIPETAMVKILEKLYEEEVGVGMYVLYPYGGIMDEISESAIPFPHRAGIMYELW 447

Query: 447 YVVGWKDQSLKAKKTHISWIREIYEYMAPFVSKSPRAAYTNYRDLDIGSNNKYGKTSYKR 506
           Y   W+ Q    K  HI+W+R +Y +  P+VS++PR AY NYRDLD+G  N     +Y +
Sbjct: 448 YTATWEKQEDNEK--HINWVRSVYNFTTPYVSQNPRLAYLNYRDLDLGKTNPESPNNYTQ 507

Query: 507 ASIWGMKYFGDNFDRLVYVKTKVDPYDFFRHEQSIPTL 538
           A IWG KYFG NF+RLV VKTK DP +FFR+EQSIP L
Sbjct: 508 ARIWGEKYFGKNFNRLVKVKTKADPNNFFRNEQSIPPL 540

BLAST of CSPI01G25140 vs. TrEMBL
Match: B9NA97_POPTR (FAD-binding domain-containing family protein OS=Populus trichocarpa GN=POPTR_0001s43120g PE=4 SV=1)

HSP 1 Score: 664.5 bits (1713), Expect = 1.1e-187
Identity = 317/530 (59.81%), Postives = 412/530 (77.74%), Query Frame = 1

Query: 10  LSLIFIIVVSPASLCASASPQKYQNFLHCLSEHSSKSYPISKVVYTPINSSYSSVLDFSI 69
           LS +F ++ S + +    S   +++FL CL   S  S+ ISK++YTPINSSYSS+L FSI
Sbjct: 2   LSFLFFLIFSFSWV---TSAHTHESFLQCLD--SQNSHSISKLIYTPINSSYSSILQFSI 61

Query: 70  RNLLFSKPETPKPLLIITPSHISHIQAAVICSKSHGLQIRTRSGGHDFEGLSYVAYRPFI 129
           +NL F+   TPKPL+I+TP++ISHIQAA+ICS+ HG+QIR RSGGHD+EGLSYV+   F+
Sbjct: 62  QNLRFNTSSTPKPLVIVTPTNISHIQAAIICSQKHGMQIRIRSGGHDYEGLSYVSTFSFV 121

Query: 130 VVDLINLRSVKVDVENNTAWVESGATLGELYYKIGEKSRTLAFPAGVCPTVGIGGHFSGG 189
           V+DLINLR++ V+ EN TAWV+SG T+GE+YY+I EKSRTLAFPA VCPTVG+GGHFSGG
Sbjct: 122 VIDLINLRTINVNAENKTAWVQSGVTIGEVYYRIAEKSRTLAFPASVCPTVGVGGHFSGG 181

Query: 190 GYGLMLRKFGLAADNVIDAYLVDAHGKVLDRESMGEDLFWAIRGGGGGSFGIVVAWKIKL 249
           GYG+++RK+GLAAD++IDA LVD  G++LDRESMGEDLFWAIRGGGG +FG+V+AWKI L
Sbjct: 182 GYGMLMRKYGLAADHIIDAQLVDVKGRILDRESMGEDLFWAIRGGGGNTFGVVIAWKISL 241

Query: 250 VRVPPTVTICSTDRNLEEDTIRLIHRWQYVGNKLDENVYLGIILTGGNASTQAGITNPT- 309
           V  PPTVT+ +  R LE++  +L+HRWQ+V +KL E++++ IIL   N ST+ G +N T 
Sbjct: 242 VSAPPTVTVFNVQRTLEQNASKLVHRWQFVADKLHEDLFIRIILNRVN-STEEGKSNTTI 301

Query: 310 -ARFFSLFLGRVDEFMATLSTTFPELGLIKQDCVEASWVESTLIIPIGVQPIESLEPLLN 369
            A F SLFLG +D  +  +  +FPELGL+K+DC+E SW++S L    G     SL+ LL+
Sbjct: 302 QASFNSLFLGGIDRLLPLIQDSFPELGLVKEDCIEMSWIQSVLYFD-GFPSNSSLDVLLD 361

Query: 370 RTPTYLDSTKIKSDYVKEPISEATIEGIWQRLKAQDIETSQVIFVPYGGRMSQISESETP 429
           RTP+   + K KSDYVKEPI E  +EGIW+R   +DI T  +IF PYGG+MS+ISES  P
Sbjct: 362 RTPSTRRNFKAKSDYVKEPIPELGLEGIWERFFDKDINTPILIFSPYGGKMSEISESSIP 421

Query: 430 FPHRAGYLFKIAYVVGWKDQSLKAKKTHISWIREIYEYMAPFVSKSPRAAYTNYRDLDIG 489
           FPHRAG ++KI +++ W ++ + A K HISWIR +Y Y+AP+VSK+PRAAY NYRDLDIG
Sbjct: 422 FPHRAGNIYKIQHLIYWDEEGIVATKRHISWIRRLYSYLAPYVSKTPRAAYVNYRDLDIG 481

Query: 490 SNNKYGKTSYKRASIWGMKYFGDNFDRLVYVKTKVDPYDFFRHEQSIPTL 538
            NN  G TSY++ASIWG+KYF +NFDRLV VKT VDP +FFR+EQSIP L
Sbjct: 482 INNHAGNTSYRQASIWGLKYFKNNFDRLVRVKTAVDPANFFRNEQSIPPL 524

BLAST of CSPI01G25140 vs. TrEMBL
Match: B9N5Q8_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0011s16580g PE=4 SV=1)

HSP 1 Score: 641.3 bits (1653), Expect = 1.0e-180
Identity = 314/534 (58.80%), Postives = 393/534 (73.60%), Query Frame = 1

Query: 4   SSTLIPLSLIFIIVVSPASLCASASPQKYQNFLHCLSEHSSKSYPISKVVYTPINSSYSS 63
           ++T++P  L F++     S   SAS   +++FL CLS +S  S   SK+VYTP NSSY  
Sbjct: 7   TTTMLPF-LCFLLF----SFLWSASAHTHEDFLQCLSLYSEDSNSFSKLVYTPNNSSYLH 66

Query: 64  VLDFSIRNLLFSKPETPKPLLIITPSHISHIQAAVICSKSHGLQIRTRSGGHDFEGLSYV 123
           VL  SI+N  F+   TPKP +I+TP ++SHIQA + CS+ HG+ +R RSGGHD+EGLSYV
Sbjct: 67  VLQGSIQNSRFNTTATPKPPVIVTPMNVSHIQATIFCSQKHGMNVRIRSGGHDYEGLSYV 126

Query: 124 AYRPFIVVDLINLRSVKVDVENNTAWVESGATLGELYYKIGEKSRTLAFPAGVCPTVGIG 183
           +  PF+++DLINLR++ VDVEN+TAWV++GATLGELYY I EKS TLAFPAG CPTVG G
Sbjct: 127 SVLPFVIIDLINLRAINVDVENSTAWVQAGATLGELYYSIAEKSGTLAFPAGACPTVGAG 186

Query: 184 GHFSGGGYGLMLRKFGLAADNVIDAYLVDAHGKVLDRESMGEDLFWAIRGGGGGSFGIVV 243
           GH SGGGYG ++RK+GLAADN+IDA L+DA G++LDR SMGEDLFWAIRGGGG +FG+VV
Sbjct: 187 GHLSGGGYGGLMRKYGLAADNIIDAQLIDAKGRILDRASMGEDLFWAIRGGGGNTFGVVV 246

Query: 244 AWKIKLVRVPPTVTICSTDRNLEEDTIRLIHRWQYVGNKLDENVYLGIILTGGNASTQAG 303
           AWK+KLV VP TVT  S  R+LEE+  +LIHRWQYV NKL E++++   +T  N S+Q G
Sbjct: 247 AWKLKLVPVPHTVTFFSVLRSLEENATKLIHRWQYVANKLPEDLFITAYITKIN-SSQEG 306

Query: 304 ITNPTARFFSLFLGRVDEFMATLSTTFPELGLIKQDCVEASWVESTLIIPIGVQPIESLE 363
           I+   A F SLFLG  D  +  +   FPELGL+K DC E SWVE  L    G     SL+
Sbjct: 307 ISTIQAEFPSLFLGGADRLLPLMQENFPELGLVKDDCTEMSWVEFVL-YNSGYSSNSSLD 366

Query: 364 PLLNRTPTYLDSTKIKSDYVKEPISEATIEGIWQRLKAQDIETSQVIFVPYGGRMSQISE 423
            LLNRTP Y+ + K KSDYVK+P+ E   EGIW+R     IET +++ VPYGG+M QISE
Sbjct: 367 VLLNRTPQYITNFKAKSDYVKKPMPEIAFEGIWKRFLKVGIETPRLVLVPYGGKMDQISE 426

Query: 424 SETPFPHRAGYLFKIAYVVGWKDQSLKAKKTHISWIREIYEYMAPFVSKSPRAAYTNYRD 483
           S  PF HRAG L+KI Y++ W +Q  +A   HI+WIR +Y Y AP+VSK+PR AY NYRD
Sbjct: 427 SSIPFAHRAGNLYKIQYLLLWNEQGKEASMRHIAWIRRLYSYTAPYVSKNPREAYINYRD 486

Query: 484 LDIGSNNKYGKTSYKRASIWGMKYFGDNFDRLVYVKTKVDPYDFFRHEQSIPTL 538
           LD+G NN  G TSYK+ASIWG KYF +NFDRL+ VKT VDP +FFR+EQSIP L
Sbjct: 487 LDVGMNNIQGNTSYKQASIWGRKYFKNNFDRLIQVKTAVDPDNFFRNEQSIPPL 533

BLAST of CSPI01G25140 vs. TrEMBL
Match: B9GH04_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0001s46910g PE=4 SV=1)

HSP 1 Score: 639.0 bits (1647), Expect = 5.1e-180
Identity = 311/533 (58.35%), Postives = 397/533 (74.48%), Query Frame = 1

Query: 5   STLIPLSLIFIIVVSPASLCASASPQKYQNFLHCLSEHSSKSYPISKVVYTPINSSYSSV 64
           ++++P  L  +I  S      SA P+  ++FL CLS H      +S  ++TP NSSYSS+
Sbjct: 6   ASMLPFLLCLLISFS---WVISAHPR--EDFLKCLSLHFEDPAAMSNAIHTPYNSSYSSI 65

Query: 65  LDFSIRNLLFSKPETPKPLLIITPSHISHIQAAVICSKSHGLQIRTRSGGHDFEGLSYVA 124
           L FSIRNL F+  E  KPL+I+TP++ SHIQAA++CS+ H LQIR RSGGHDFEGLSY+A
Sbjct: 66  LQFSIRNLRFNSSEL-KPLVIVTPTNASHIQAAILCSQRHNLQIRIRSGGHDFEGLSYMA 125

Query: 125 YRPFIVVDLINLRSVKVDVENNTAWVESGATLGELYYKIGEKSRTLAFPAGVCPTVGIGG 184
             PF+++DLI+LR+V VD  + TAWV++GATLGELYY I EKSRTLAFPAG CPT+G+GG
Sbjct: 126 ALPFVIIDLISLRAVNVDATSRTAWVQAGATLGELYYSISEKSRTLAFPAGSCPTIGVGG 185

Query: 185 HFSGGGYGLMLRKFGLAADNVIDAYLVDAHGKVLDRESMGEDLFWAIRGGGGGSFGIVVA 244
           HFSGGG+G M+RKFGLA+DNVIDA+L+D+ G++LDR SMGEDLFWAIRGGGG SFG+VVA
Sbjct: 186 HFSGGGHGTMVRKFGLASDNVIDAHLIDSKGRILDRASMGEDLFWAIRGGGGQSFGVVVA 245

Query: 245 WKIKLVRVPPTVTICSTDRNLEEDTIRLIHRWQYVGNKLDENVYLGIILTGGNASTQAGI 304
           WKI LV VP TVT+ S  R LE++  +L+HRWQYV N L E++ + + +T  N S+Q G 
Sbjct: 246 WKISLVEVPSTVTMFSVSRTLEQNATKLLHRWQYVANTLPEDLVIDVQVTRVN-SSQEGN 305

Query: 305 TNPTARFFSLFLGRVDEFMATLSTTFPELGLIKQDCVEASWVESTLIIPIGVQPIESLEP 364
           T   A FFSLFLG VD+ +  +  +FPELGL+K DC E SW+ES      G     SL+ 
Sbjct: 306 TTIQATFFSLFLGEVDQLLPVMQESFPELGLVKDDCFEMSWIESVFYTG-GFTSNASLDV 365

Query: 365 LLNRTPTYLDSTKIKSDYVKEPISEATIEGIWQRLKAQDIETSQVIFVPYGGRMSQISES 424
           LLNRTP  +   K KSDYVKEP+ E   EGIW+R   +DIE   +I +PYGG+M +ISES
Sbjct: 366 LLNRTPRSIPRFKAKSDYVKEPMPEIAFEGIWERFFEEDIEAPTLILIPYGGKMDEISES 425

Query: 425 ETPFPHRAGYLFKIAYVVGWKDQSLKAKKTHISWIREIYEYMAPFVSKSPRAAYTNYRDL 484
            TPFPHRAG L+ +   V W+++S +A + H++WIR +Y Y+  +VSK+PR AY NYRDL
Sbjct: 426 STPFPHRAGNLYVLVSSVSWREESKEASRRHMAWIRRLYSYLTKYVSKNPREAYVNYRDL 485

Query: 485 DIGSNNKYGKTSYKRASIWGMKYFGDNFDRLVYVKTKVDPYDFFRHEQSIPTL 538
           D+G NN  G TSYK+ASIWG KYF +NFDRLV VKT+VDP +FFR+EQSIP+L
Sbjct: 486 DLGINNLTGTTSYKQASIWGRKYFKNNFDRLVRVKTEVDPTNFFRNEQSIPSL 530

BLAST of CSPI01G25140 vs. TrEMBL
Match: A0A061GF79_THECC (FAD-binding Berberine family protein OS=Theobroma cacao GN=TCM_027038 PE=4 SV=1)

HSP 1 Score: 638.6 bits (1646), Expect = 6.6e-180
Identity = 305/541 (56.38%), Postives = 401/541 (74.12%), Query Frame = 1

Query: 1   MKFSSTLIPLSLIFIIVVSPASLCASASPQKYQNFLHCLSEHSSKSYPISKVVYTPINSS 60
           MK  S  +PL L+ +   S A+     S + + +FL CLS HS  +   ++++YT  +SS
Sbjct: 1   MKSPSFSVPLFLLVLFSFSWAT-----SARTHDSFLQCLSLHSGIN---ARLIYTQNSSS 60

Query: 61  YSSVLDFSIRNLLFSKPETPKPLLIITPSHISHIQAAVICSKSHGLQIRTRSGGHDFEGL 120
           Y SVLD S RN  FS P TP+PL+I+TPS++SH+QA + CS+ H +QIR RSGGHD+EGL
Sbjct: 61  YESVLDLSARNARFSTPTTPRPLVIVTPSNVSHVQATINCSREHDMQIRIRSGGHDYEGL 120

Query: 121 SYVAYRPFIVVDLINLRSVKVDVENNTAWVESGATLGELYYKIGEKSRTLAFPAGVCPTV 180
           SYV+  PF+++DLIN+RS+ VD E  TAW+++GAT+GELYY+I EKS TLAFPAGVCPTV
Sbjct: 121 SYVSKVPFVIIDLINMRSIDVDTEKKTAWIQAGATIGELYYRIAEKSTTLAFPAGVCPTV 180

Query: 181 GIGGHFSGGGYGLMLRKFGLAADNVIDAYLVDAHGKVLDRESMGEDLFWAIRGGGGGSFG 240
           G+GGHFSGGGYG+M RKFGLAAD +IDA++V   G++LDR SMGEDLFWAIRGGGG SFG
Sbjct: 181 GVGGHFSGGGYGMMTRKFGLAADQIIDAHIVTVDGRILDRNSMGEDLFWAIRGGGGASFG 240

Query: 241 IVVAWKIKLVRVPPTVTICSTDRNLEEDTIRLIHRWQYVGNKLDENVYLGIILTGGNAST 300
           ++VAWK+KLV VP TVT+ + +R LEE+  +L+H+WQ V   +DE++Y  + L   N S+
Sbjct: 241 VIVAWKVKLVSVPSTVTVFTVNRTLEENATKLVHKWQSVAEAIDEDLYFRVFLRAVN-SS 300

Query: 301 QAGITNPTARFFSLFLGRVDEFMATLSTTFPELGLIKQDCVEASWVESTLIIPIGVQPIE 360
           Q G     A F SLFLGR+D+ +A +  +FPELGL+K+DC+E SW++S L  P G+   E
Sbjct: 301 QEGKRTVQASFTSLFLGRIDQLIALMQESFPELGLVKEDCIEMSWIQSILYFP-GIPQSE 360

Query: 361 SLEPLLNRTPTYLDST----KIKSDYVKEPISEATIEGIWQRLKAQDIETSQVIFVPYGG 420
           SL+ LLNRT T    T    K KSDYV EPI E  +EG+WQ     +  T ++IF PYGG
Sbjct: 361 SLDVLLNRTGTIRTVTAGIFKAKSDYVTEPIPEIALEGLWQWFYEDESGTLELIFSPYGG 420

Query: 421 RMSQISESETPFPHRAGYLFKIAYVVGWKDQSLKAKKTHISWIREIYEYMAPFVSKSPRA 480
            M +ISESETPFPHRAG L+KI Y V W+++     + HI+++R +Y+YM P+VSKSPRA
Sbjct: 421 IMDEISESETPFPHRAGNLYKIHYAVSWEEEDASTSQKHINFMRRLYKYMEPYVSKSPRA 480

Query: 481 AYTNYRDLDIGSNNKYGKTSYKRASIWGMKYFGDNFDRLVYVKTKVDPYDFFRHEQSIPT 538
           AY NYRDLD+GSNN+   TSY +AS W +KYF +NF+RL++VK+ VDP +FFRHEQS+PT
Sbjct: 481 AYMNYRDLDLGSNNEGNYTSYTQASRWALKYFKNNFNRLIHVKSMVDPQNFFRHEQSVPT 531

BLAST of CSPI01G25140 vs. TrEMBL
Match: G8A1B3_MEDTR (Reticuline oxidase OS=Medicago truncatula GN=MTR_4g094488 PE=4 SV=1)

HSP 1 Score: 637.9 bits (1644), Expect = 1.1e-179
Identity = 319/541 (58.96%), Postives = 396/541 (73.20%), Query Frame = 1

Query: 7   LIPLSLIFIIVV-----SPASLCASASPQKYQNFLHCLSEHSSKSYPISKVVYTPINSSY 66
           ++PL L   IV+     S  S     SP +  NFL CL  +S     ISKVVYT  NSSY
Sbjct: 1   MMPLRLYLTIVLIAIAFSFTSFAIDTSPHE-DNFLQCLYSYSHNITSISKVVYTKTNSSY 60

Query: 67  SSVLDFSIRNLLFSKPETPKPLLIITPSHISHIQAAVICSKSHGLQIRTRSGGHDFEGLS 126
           SS+L FSI+NL F+  ETPKPL+IITP+ ISHIQ A+ICS+ HG+QIR RSGGHDFEGLS
Sbjct: 61  SSILKFSIQNLRFATNETPKPLVIITPTQISHIQTAIICSQHHGMQIRIRSGGHDFEGLS 120

Query: 127 YVAYRPFIVVDLINLRSVKVDVENNTAWVESGATLGELYYKIGEKSRTLAFPAGVCPTVG 186
           +V+  PF+++DL N R + VDVEN TAWV+SGATLGELYYKI +KS+TL FP GVCPTVG
Sbjct: 121 FVSNVPFVIIDLTNFRGIDVDVENRTAWVQSGATLGELYYKIAQKSKTLGFPGGVCPTVG 180

Query: 187 IGGHFSGGGYGLMLRKFGLAADNVIDAYLVDAHGKVLDRESMGEDLFWAIRGGGGGSFGI 246
           +GGHFSGGGYG +LRK+GLAADNVIDA+++D  G+ LDRE+MGEDLFWAIRGGGG SFG+
Sbjct: 181 VGGHFSGGGYGTLLRKYGLAADNVIDAHIIDVKGRFLDREAMGEDLFWAIRGGGGASFGV 240

Query: 247 VVAWKIKLVRVPPTVTICSTDRNLEEDTIRLIHRWQYVGNKLDENVYLGIILTGGNA-ST 306
           +V+WKIKLV+VP TVT+ +  R LE++  +L+H+WQ+V +KL+EN+ + IIL   +  S+
Sbjct: 241 IVSWKIKLVQVPSTVTVFTVPRTLEQNATKLVHKWQFVAHKLEENLAINIILQRLDLNSS 300

Query: 307 QAGITNPT--ARFFSLFLGRVDEFMATLSTTFPELGLIKQDCVEASWVESTLII---PIG 366
           + G    T  A F SLFLG VD  +  +   FPELGL+++DCVE SW+ES L +   P G
Sbjct: 301 KQGEPKSTVLALFQSLFLGSVDNLLPLMEEKFPELGLVREDCVEMSWIESVLYLFRFPEG 360

Query: 367 VQPIESLEPLLNRTPTYLDSTKIKSDYVKEPISEATIEGIWQRLKAQDIETSQVIFVPYG 426
               E LE LLNRT    D++K KSD+VK PI E  +EG+W        E   ++  PYG
Sbjct: 361 ----EPLETLLNRTLAAKDNSKAKSDFVKIPIPETGLEGLWPLFDEDGAEDVLMVLFPYG 420

Query: 427 GRMSQISESETPFPHRAGYLFKIAYVVGWKDQSLKAKKTHISWIREIYEYMAPFVSKSPR 486
           G M +ISESE PFPHR G L+KI Y V W  +  + +K HI+WIR++Y YM PFVSKSPR
Sbjct: 421 GIMDKISESEIPFPHRYGTLYKIQYAVHWHQEGDEVEKLHINWIRKLYSYMEPFVSKSPR 480

Query: 487 AAYTNYRDLDIGSNNKYGKTSYKRASIWGMKYFGDNFDRLVYVKTKVDPYDFFRHEQSIP 537
           AAY NYRDLDIG NN  G TSYK+ASIWG+KYF +NF RL  VKTKVDP +FFR+EQSIP
Sbjct: 481 AAYINYRDLDIGVNNINGYTSYKQASIWGVKYFKNNFKRLAKVKTKVDPLNFFRNEQSIP 536

BLAST of CSPI01G25140 vs. TAIR10
Match: AT5G44440.1 (AT5G44440.1 FAD-binding Berberine family protein)

HSP 1 Score: 531.2 bits (1367), Expect = 7.5e-151
Identity = 264/542 (48.71%), Postives = 373/542 (68.82%), Query Frame = 1

Query: 1   MKFSSTLIPLSLIFIIVVSPASLCASASPQKYQNFLHCLSEHSSKSYPISKVVYTPINSS 60
           M+FSS L  + L+F + +SP     SA    +++FL CLS   + +    KV++T  +SS
Sbjct: 1   MEFSSFLFTI-LLFSLNISPL---VSAHGSNHEDFLKCLSYRMNDNTVEPKVIHTSKDSS 60

Query: 61  YSSVLDFSIRNLLFSKPETPKPLLIITPSHISHIQAAVICSKSHGLQIRTRSGGHDFEGL 120
           + S+LD SI+N  FS  ETPKP+ IITP   S +Q  + C++ HG+ +RTRS GH +EGL
Sbjct: 61  FFSILDSSIQNPRFSVSETPKPVSIITPVKASDVQTVIRCAQLHGIHVRTRSAGHCYEGL 120

Query: 121 SYVAY-RPFIVVDLINLRSVKVDVENNTAWVESGATLGELYYKIGEKSRTLAFPAGVCPT 180
           SY+AY +PF V+DL NLRS+ +DV+N T WV++GAT GELYY+IG+ +++LAFPAG+ PT
Sbjct: 121 SYIAYNKPFAVIDLRNLRSISLDVDNRTGWVQTGATAGELYYEIGKTTKSLAFPAGIHPT 180

Query: 181 VGIGGHFSGGGYGLMLRKFGLAADNVIDAYLVDAHGKVLDRESMGEDLFWAIRGGGGGSF 240
           VG+GG FSGGGYG +LRK+GLAADN+IDA +VDA G++LDR++MGED FWAIRGGGG SF
Sbjct: 181 VGVGGQFSGGGYGTLLRKYGLAADNIIDALVVDASGRILDRQAMGEDYFWAIRGGGGSSF 240

Query: 241 GIVVAWKIKLVRVPPTVTICSTDRNLEEDTIRLIHRWQYVGNKLDENVYLGIILTGGNAS 300
           G++++WK+KLV VP T+T+    +  +++ +R+I +WQY  +K+ +++++   L   N +
Sbjct: 241 GVILSWKVKLVDVPSTITVFKVQKTSKKEAVRIIKKWQYAADKVPDDLFIRTTLERSNKN 300

Query: 301 TQAGITNPTARFFSLFLGRVDEFMATLSTTFPELGLIKQDCVEASWVESTL---IIPIGV 360
                    A F  L++G V+  +A +   FPELGL K+ C E SW+ES L     P G 
Sbjct: 301 A------VHALFTGLYIGPVNNLLALMEEKFPELGLEKEGCEEMSWIESVLWFADFPKG- 360

Query: 361 QPIESLEPLLNRTPTYLDSTKIKSDYVKEPISEATIEGIWQRLKAQDIETSQVIFVPYGG 420
              ESL  L NR  T L S K K D+V+EPI EA I+ IW+RL+A +    ++I  P+GG
Sbjct: 361 ---ESLGVLTNRERTSL-SFKGKDDFVQEPIPEAAIQEIWRRLEAPEARLGKIILTPFGG 420

Query: 421 RMSQISESETPFPHRAGYLFKIAYVVGWKDQSLKAK---KTHISWIREIYEYMAPFVSKS 480
           +MS+++E ETPFPHR G L++I YV  W+++  K K     ++ W+  +YE+M P+VSKS
Sbjct: 421 KMSEMAEYETPFPHRGGNLYEIQYVAYWREEEDKNKTETDKYLKWVDSVYEFMTPYVSKS 480

Query: 481 PRAAYTNYRDLDIGSNNKYGKTSYKRASIWGMKYFGDNFDRLVYVKTKVDPYDFFRHEQS 536
           PR AY N++D+D+G      KT Y+    WG+KYF +NF+RLV VKT+VDP DFF  EQS
Sbjct: 481 PRGAYVNFKDMDLGMYLGKKKTKYEEGKSWGVKYFKNNFERLVRVKTRVDPTDFFCDEQS 527

BLAST of CSPI01G25140 vs. TAIR10
Match: AT4G20820.1 (AT4G20820.1 FAD-binding Berberine family protein)

HSP 1 Score: 531.2 bits (1367), Expect = 7.5e-151
Identity = 262/534 (49.06%), Postives = 373/534 (69.85%), Query Frame = 1

Query: 3   FSSTLIPLSLIFIIVVSPASLCASASPQKYQNFLHCLSEHSSKSYPISKVVYTPINSSYS 62
           FSS LI  +   +++  P  + A+ S Q   +FL CLS   + S  +SKV++TP ++S+S
Sbjct: 7   FSSVLIFFTTSTLLLSIPHPVSANRSNQS--SFLQCLSLQLNDSNIVSKVIHTPNDTSFS 66

Query: 63  SVLDFSIRNLLFSKPETPKPLLIITPSHISHIQAAVICSKSHGLQIRTRSGGHDFEGLSY 122
           SVL  SI+N  FS P+ PKP+LI+TP   S +Q+AV C++  G+ IRTRSGGHD+EGLSY
Sbjct: 67  SVLASSIQNQRFSAPDVPKPVLILTPVQPSDVQSAVKCARRFGIHIRTRSGGHDYEGLSY 126

Query: 123 VAYRPFIVVDLINLRSVKVDVENNTAWVESGATLGELYYKIGEKSRTLAFPAGVCPTVGI 182
           V ++PF+++DL NLRS+ VDV+N + WV++GAT+GELYY+IG+K+RTLAFPAGVCPTVG+
Sbjct: 127 VTHKPFVILDLRNLRSITVDVDNRSVWVQTGATIGELYYEIGKKNRTLAFPAGVCPTVGV 186

Query: 183 GGHFSGGGYGLMLRKFGLAADNVIDAYLVDAHGKVLDRESMGEDLFWAIRGGGGGSFGIV 242
           GGHFSGGGYG +LRK GLAAD+VIDA +VDA G++L+R  MGED FWAIRGGGG SF +V
Sbjct: 187 GGHFSGGGYGTLLRKHGLAADHVIDARVVDARGRILERREMGEDFFWAIRGGGGSSFCVV 246

Query: 243 VAWKIKLVRVPPTVTICSTDRNLEEDTIRLIHRWQYVGNKLDENVYLGIILTGGNASTQA 302
           ++WKI L+ VP TVT+ +  +  E+  +++IHRWQ+V +K+ +++++ ++L       Q 
Sbjct: 247 LSWKIGLINVPSTVTVFNVTKFSEQSALKIIHRWQFVADKVSDDLFIRVML-------QR 306

Query: 303 GITNPTARFFSLFLGRVDEFMATLSTTFPELGLIKQDCVEASWVESTL-IIPIGVQPIES 362
                 A F  L+LG V   +  ++  FPELGL + DC E SW+ES +    +G +PI  
Sbjct: 307 YKNMVRASFPGLYLGSVKNLLKMVNKEFPELGLEEDDCTEMSWIESVIWFAELGEEPI-- 366

Query: 363 LEPLLNRTPTYLDSTKIKSDYVKEPISEATIEGIWQRLKAQDIETSQVIFVPYGGRMSQI 422
              L  RT   L + K KSD+V+EP+ +  I  +W+RL+  + E +Q+IF P+GG+MS+I
Sbjct: 367 -NVLTKRTRASL-AFKAKSDFVQEPMPKTAISKLWRRLQEPEAEHAQLIFTPFGGKMSEI 426

Query: 423 SESETPFPHRAGYLFKIAYVVGWKDQSLKAKKTHISWIREIYEYMAPFVSKSPRAAYTNY 482
           ++ ETPFPHR G +++I Y+  W+      K+ ++ W+  +Y+ M+ FV+KSPR AY N 
Sbjct: 427 ADYETPFPHRKGNIYEIQYLNYWRGD---VKEKYMRWVERVYDDMSEFVAKSPRGAYINL 486

Query: 483 RDLDIGSNNKYGKTSYKRASIWGMKYFGDNFDRLVYVKTKVDPYDFFRHEQSIP 536
           RDLD+G      ++ Y+    WG+KYF +NF+RLV VKT VDP DFF  EQSIP
Sbjct: 487 RDLDLGMYVGVKRSKYEEGKSWGVKYFKNNFERLVRVKTSVDPSDFFCDEQSIP 524

BLAST of CSPI01G25140 vs. TAIR10
Match: AT1G30700.1 (AT1G30700.1 FAD-binding Berberine family protein)

HSP 1 Score: 521.5 bits (1342), Expect = 6.0e-148
Identity = 264/541 (48.80%), Postives = 365/541 (67.47%), Query Frame = 1

Query: 1   MKFSSTLIPLSLIFIIVVSPASLCASASPQKYQNFLHCLSEHSSKSYPISKVVYTPINSS 60
           MK++  L+   ++FI         +S+S    + F  CL+ +S   +PIS  ++   N S
Sbjct: 1   MKYALILVLFFVVFIWQ-------SSSSSANSETFTQCLTSNSDPKHPISPAIFFSGNGS 60

Query: 61  YSSVLDFSIRNLLFSKPETPKPLLIITPSHISHIQAAVICSKSHGLQIRTRSGGHDFEGL 120
           YSSVL  +IRNL F+   TPKP LII  +H SH+QAA+ C K H LQ++ RSGGHD++GL
Sbjct: 61  YSSVLQANIRNLRFNTTSTPKPFLIIAATHESHVQAAITCGKRHNLQMKIRSGGHDYDGL 120

Query: 121 SYVAY--RPFIVVDLINLRSVKVDVENNTAWVESGATLGELYYKIGEKSRTLAFPAGVCP 180
           SYV Y  +PF V+D+ NLRSV VDV + TAWV++GA LGE+YY I EKS+TLA+PAG+CP
Sbjct: 121 SYVTYSGKPFFVLDMFNLRSVDVDVASKTAWVQTGAILGEVYYYIWEKSKTLAYPAGICP 180

Query: 181 TVGIGGHFSGGGYGLMLRKFGLAADNVIDAYLVDAHGKVLDRESMGEDLFWAIRGGGGGS 240
           TVG+GGH SGGGYG M+RK+GL  DN IDA +VD +GK+LDR+ MGEDL+WAI GGGGGS
Sbjct: 181 TVGVGGHISGGGYGNMMRKYGLTVDNTIDARMVDVNGKILDRKLMGEDLYWAINGGGGGS 240

Query: 241 FGIVVAWKIKLVRVPPTVTICSTDRNLEEDTIRLIHRWQYVGNKLDENVYLGIILTGGNA 300
           +G+V+A+KI LV VP  VT+    R LE++   +IHRWQ V  KL + +++  ++   N 
Sbjct: 241 YGVVLAYKINLVEVPENVTVFRISRTLEQNATDIIHRWQQVAPKLPDELFIRTVIDVVNG 300

Query: 301 STQAGITNPTARFFSLFLGRVDEFMATLSTTFPELGLIKQDCVEASWVESTLI-IPIGVQ 360
           +  +  T  T  F ++FLG     ++ L+  FPELGL++ DC E SW++S L    I V 
Sbjct: 301 TVSSQKTVRTT-FIAMFLGDTTTLLSILNRRFPELGLVRSDCTETSWIQSVLFWTNIQVG 360

Query: 361 PIESLEPLLNRTPTYLDSTKIKSDYVKEPISEATIEGIWQRLKAQDIETSQVIFVPYGGR 420
             E+L    N+   YL   K KSDYV+EPIS   +E IW+++   +I T  + F PYGG 
Sbjct: 361 SSETLLLQRNQPVNYL---KRKSDYVREPISRTGLESIWKKMIELEIPT--MAFNPYGGE 420

Query: 421 MSQISESETPFPHRAGYLFKIAYVVGWKDQSLKAKKTHISWIREIYEYMAPFVSKSPRAA 480
           M +IS + TPFP+RAG L+KI Y   W+D++L  +   ++  R++Y++M PFVSK+PR +
Sbjct: 421 MGRISSTVTPFPYRAGNLWKIQYGANWRDETLTDRYMELT--RKLYQFMTPFVSKNPRQS 480

Query: 481 YTNYRDLDIGSNNKYGK-TSYKRASIWGMKYFGDNFDRLVYVKTKVDPYDFFRHEQSIPT 538
           + NYRD+D+G N+  GK +SY     +G KYF  NF+RLV +KT+VD  +FFR+EQSIP 
Sbjct: 481 FFNYRDVDLGINSHNGKISSYVEGKRYGKKYFAGNFERLVKIKTRVDSGNFFRNEQSIPV 526

BLAST of CSPI01G25140 vs. TAIR10
Match: AT5G44400.1 (AT5G44400.1 FAD-binding Berberine family protein)

HSP 1 Score: 511.5 bits (1316), Expect = 6.2e-145
Identity = 258/541 (47.69%), Postives = 361/541 (66.73%), Query Frame = 1

Query: 9   PLSLIFIIV-------VSPASLCASASPQKYQNFLHCLSEHSSKSYPISKVVYTPIN--S 68
           PL L  I+V       ++P S  AS   Q    F++C+  ++   +P+ K  + P    S
Sbjct: 6   PLPLFSILVLYFSLYTITPTSSLASLQDQ----FINCVQRNTHVYFPLEKTFFAPTKNVS 65

Query: 69  SYSSVLDFSIRNLLFSKPETPKPLLIITPSHISHIQAAVICSKSHGLQIRTRSGGHDFEG 128
            +S VL+ + +NL F K   PKP  I +P H SH+QA++ICSK   + +R RSGGHD+EG
Sbjct: 66  MFSQVLESTAQNLRFLKKSMPKPGFIFSPIHESHVQASIICSKKLRMHLRVRSGGHDYEG 125

Query: 129 LSYVAY--RPFIVVDLINLRSVKVDVENNTAWVESGATLGELYYKIGEKSRTLAFPAGVC 188
           LSYV+   +PFI++DL  +R V +++++N+AWV+SGAT+GELYY+I EKS+   FPAG+C
Sbjct: 126 LSYVSQIDKPFILMDLSKMRQVNINIQDNSAWVQSGATVGELYYRIAEKSKVHGFPAGLC 185

Query: 189 PTVGIGGHFSGGGYGLMLRKFGLAADNVIDAYLVDAHGKVLDRESMGEDLFWAIRGGGGG 248
            ++GIGGH +GG YG M+RK+GL ADNV+DA +VDA+GK+LDR +MGED FWAIRGG GG
Sbjct: 186 SSLGIGGHITGGAYGSMMRKYGLGADNVLDAKIVDANGKLLDRAAMGEDTFWAIRGGAGG 245

Query: 249 SFGIVVAWKIKLVRVPPTVTICSTDRNLEEDT-IRLIHRWQYVGNKLDENVYLGIILTGG 308
           SFGI++AWKIKLV VP TVT+ +  + L++D   ++I +WQ V +KL E +++ ++    
Sbjct: 246 SFGIILAWKIKLVPVPKTVTVFTVTKTLQQDVGNKIISKWQRVADKLVEELFIRVLF--- 305

Query: 309 NASTQAGITNPTARFFSLFLGRVDEFMATLSTTFPELGLIKQDCVEASWVESTLIIPIGV 368
           N +   G    T  + +LFLG     M  +  +FPELGL  +DC+E SW+ES   I    
Sbjct: 306 NVAGTGGNKTVTTSYNALFLGGKGTLMNVMKKSFPELGLTFKDCIEMSWLESIAYISGFP 365

Query: 369 QPIESLEPLLNRTPTYLDSTKIKSDYVKEPISEATIEGIWQRLKAQDIETSQVIFVPYGG 428
               +   L  ++P    S K KSD+VK PI E+ ++GI+++L  +DI    +I+ PYGG
Sbjct: 366 THTPTNVLLQGKSPFPKVSFKAKSDFVKTPIPESGLQGIFKKLLKEDIPL--MIWNPYGG 425

Query: 429 RMSQISESETPFPHRAGYLFKIAYVVGWKDQSLKAKKTHISWIREIYEYMAPFVSKSPRA 488
            M++I ES+ PFPHR G LFK+ YV  W D S K    HI+WIR++Y YM P+VS +PR 
Sbjct: 426 MMAKIPESQIPFPHRKGVLFKVQYVTSWLD-SDKRPSRHINWIRDLYSYMTPYVSSNPRE 485

Query: 489 AYTNYRDLDIGSNNKYGKTSYKRASIWGMKYFGDNFDRLVYVKTKVDPYDFFRHEQSIPT 538
           AY NYRDLD+G N K  KT  K+A +WG  YF +NF+RL+ +K KVDP +FFRHEQSIP 
Sbjct: 486 AYVNYRDLDLGRNTKDVKTCIKQAQVWGANYFKNNFNRLMMIKAKVDPENFFRHEQSIPP 536

BLAST of CSPI01G25140 vs. TAIR10
Match: AT5G44380.1 (AT5G44380.1 FAD-binding Berberine family protein)

HSP 1 Score: 507.7 bits (1306), Expect = 8.9e-144
Identity = 262/545 (48.07%), Postives = 355/545 (65.14%), Query Frame = 1

Query: 4   SSTLIPLSLIFIIVVSPASLC---ASASPQKYQNFLHCLSEHSSKSYPISKVVYTPIN-- 63
           S  L  +S I + V+  +  C    S+S      F++C+  ++  S+P+ K ++TP    
Sbjct: 4   SKPLPTISCIIVSVLYFSFYCITPTSSSASIQDQFINCVKRNTHVSFPLEKTLFTPAKNV 63

Query: 64  SSYSSVLDFSIRNLLFSKPETPKPLLIITPSHISHIQAAVICSKSHGLQIRTRSGGHDFE 123
           S ++ VL+ + +NL F     PKP  I  P H S +QA++ICSK  G+  R RSGGHDFE
Sbjct: 64  SLFNQVLESTAQNLQFLAKSMPKPGFIFRPIHQSQVQASIICSKKLGIHFRVRSGGHDFE 123

Query: 124 GLSYVAY--RPFIVVDLINLRSVKVDVENNTAWVESGATLGELYYKIGEKSRTLAFPAGV 183
            LSYV+   +PFI++DL  L+ + VD+E+N+AWV+ GATLGELYY+I EKS+   FPAG+
Sbjct: 124 ALSYVSRIEKPFILLDLSKLKQINVDIESNSAWVQPGATLGELYYRIAEKSKIHGFPAGL 183

Query: 184 CPTVGIGGHFSGGGYGLMLRKFGLAADNVIDAYLVDAHGKVLDRESMGEDLFWAIRGGGG 243
           C +VGIGG+ +GGGYG ++RK+GLA DNV+D  +VDA+GK+LDR +MGEDLFWAIRGGGG
Sbjct: 184 CTSVGIGGYMTGGGYGTLMRKYGLAGDNVLDVKMVDANGKLLDRAAMGEDLFWAIRGGGG 243

Query: 244 GSFGIVVAWKIKLVRVPPTVTICSTDRNLEEDT-IRLIHRWQYVGNKLDENVYLGIILTG 303
            SFGIV+AWKIKLV VP TVT+ +  + LE+D  ++ I +WQ + +K+ E +++ ++L  
Sbjct: 244 ASFGIVLAWKIKLVPVPKTVTVFTVTKTLEQDARLKTISKWQQISSKIIEEIHIRVVL-- 303

Query: 304 GNASTQAGITNPTARFFSLFLGRVDEFMATLSTTFPELGLIKQDCVEASWVESTLI---I 363
             A+   G    T  +   FLG     +  +   FPELGL ++DC E SW+E+ L     
Sbjct: 304 -RAAGNDGNKTVTMTYLGQFLGEKGTLLKVMEKAFPELGLTQKDCTEMSWIEAALFHGGF 363

Query: 364 PIGVQPIESLEPLLNRTPTYLDSTKIKSDYVKEPISEATIEGIWQRLKAQDIETSQVIFV 423
           P G  PIE L  L  ++P   D  K  SD+VKEPI    ++GI++RL   +  T+ + + 
Sbjct: 364 PTG-SPIEILLQL--KSPLGKDYFKATSDFVKEPIPVIGLKGIFKRLIEGN--TTFLNWT 423

Query: 424 PYGGRMSQISESETPFPHRAGYLFKIAYVVGWKDQSLKAKKTHISWIREIYEYMAPFVSK 483
           PYGG MS+I ES  PFPHR G LFKI Y   W +   K     I+WI+EIY YMAP+VS 
Sbjct: 424 PYGGMMSKIPESAIPFPHRNGTLFKILYYANWLEND-KTSSRKINWIKEIYNYMAPYVSS 483

Query: 484 SPRAAYTNYRDLDIGSNNKYGKTSYKRASIWGMKYFGDNFDRLVYVKTKVDPYDFFRHEQ 538
           +PR AY NYRDLD G N    K ++  A IWG KYF  NFDRLV +KTKVDP +FFRHEQ
Sbjct: 484 NPRQAYVNYRDLDFGQNKNNAKVNFIEAKIWGPKYFKGNFDRLVKIKTKVDPENFFRHEQ 539

BLAST of CSPI01G25140 vs. NCBI nr
Match: gi|659068687|ref|XP_008445684.1| (PREDICTED: tetrahydrocannabinolic acid synthase-like [Cucumis melo])

HSP 1 Score: 1010.0 bits (2610), Expect = 1.6e-291
Identity = 496/537 (92.36%), Postives = 515/537 (95.90%), Query Frame = 1

Query: 1   MKFSSTLIPLSLIFIIVVSPASLCASASPQKYQNFLHCLSEHSSKSYPISKVVYTPINSS 60
           MKFSSTLIPLSL+FI VVS ASLCASASPQKYQNFL CLSEHSSKSYPISKVVYTPINSS
Sbjct: 1   MKFSSTLIPLSLVFI-VVSSASLCASASPQKYQNFLQCLSEHSSKSYPISKVVYTPINSS 60

Query: 61  YSSVLDFSIRNLLFSKPETPKPLLIITPSHISHIQAAVICSKSHGLQIRTRSGGHDFEGL 120
           YSSVLDFSIRNLLFSKPETPKPLLIITPSH+SHIQAAVICSKSHGLQIRTRSGGHDFEGL
Sbjct: 61  YSSVLDFSIRNLLFSKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGL 120

Query: 121 SYVAYRPFIVVDLINLRSVKVDVENNTAWVESGATLGELYYKIGEKSRTLAFPAGVCPTV 180
           SYVAYRPFIVVDLINLRSVKV+VENNTAWVESGATLGELYY+IGEKSRTLAFPAGVCPTV
Sbjct: 121 SYVAYRPFIVVDLINLRSVKVEVENNTAWVESGATLGELYYRIGEKSRTLAFPAGVCPTV 180

Query: 181 GIGGHFSGGGYGLMLRKFGLAADNVIDAYLVDAHGKVLDRESMGEDLFWAIRGGGGGSFG 240
           GIGGHFSGGGYGLMLRKFGLAADNVIDAYLVDA+GKVLD+ESMGEDLFWAIRGGGGGSFG
Sbjct: 181 GIGGHFSGGGYGLMLRKFGLAADNVIDAYLVDANGKVLDKESMGEDLFWAIRGGGGGSFG 240

Query: 241 IVVAWKIKLVRVPPTVTICSTDRNLEEDTIRLIHRWQYVGNKLDENVYLGIILTGGNAST 300
           IVVAWKIKLV VP TVTICSTDRNLEED I+LIHRWQYVGNKLDEN+YLGIILTGGNAST
Sbjct: 241 IVVAWKIKLVPVPETVTICSTDRNLEEDAIKLIHRWQYVGNKLDENIYLGIILTGGNAST 300

Query: 301 QAGITNPTARFFSLFLGRVDEFMATLSTTFPELGLIKQDCVEASWVESTLIIPIGVQPIE 360
           QAGITNPTARFFSLFLGR DE + TLST FPELGLIKQDCVEASWVESTLIIPIGVQPIE
Sbjct: 301 QAGITNPTARFFSLFLGRADELVTTLSTIFPELGLIKQDCVEASWVESTLIIPIGVQPIE 360

Query: 361 SLEPLLNRTPTYLDSTKIKSDYVKEPISEATIEGIWQRLKAQDIETSQVIFVPYGGRMSQ 420
           +LEPLLNRT T LDSTKIKSDY+ EPI  A I+ IWQRLK QDIETSQ++FVPYGGRMSQ
Sbjct: 361 TLEPLLNRTLTTLDSTKIKSDYITEPIPVAAIKDIWQRLKTQDIETSQLVFVPYGGRMSQ 420

Query: 421 ISESETPFPHRAGYLFKIAYVVGWKDQSLKAKKTHISWIREIYEYMAPFVSKSPRAAYTN 480
           ISESETPF HRA YLFKI YVVGWKDQSLKAKK HISW+RE+YEYMAPFVSKSPRAAYTN
Sbjct: 421 ISESETPFSHRARYLFKIGYVVGWKDQSLKAKKRHISWLREVYEYMAPFVSKSPRAAYTN 480

Query: 481 YRDLDIGSNNKYGKTSYKRASIWGMKYFGDNFDRLVYVKTKVDPYDFFRHEQSIPTL 538
           YRDLDIGSNNKYGKTSYKRAS+WG+KYFG+NFDRLVYVKTKVDPYDFFRHEQSIPTL
Sbjct: 481 YRDLDIGSNNKYGKTSYKRASVWGLKYFGNNFDRLVYVKTKVDPYDFFRHEQSIPTL 536

BLAST of CSPI01G25140 vs. NCBI nr
Match: gi|778661997|ref|XP_004151192.2| (PREDICTED: cannabidiolic acid synthase-like [Cucumis sativus])

HSP 1 Score: 917.1 bits (2369), Expect = 1.4e-263
Identity = 446/538 (82.90%), Postives = 493/538 (91.64%), Query Frame = 1

Query: 1   MKFSSTLIPLSLIFIIVVSPASLCASASPQKYQNFLHCLSEHSSKSYPISKVVYTPINSS 60
           MK+SS LIP SLI +IVVS +SL  SASP K+ NFL CLSEHSSKSYPISKVV+TPINSS
Sbjct: 1   MKYSSPLIPFSLI-LIVVSSSSLWVSASPHKHHNFLQCLSEHSSKSYPISKVVHTPINSS 60

Query: 61  YSSVLDFSIRNLLFSKPETPKPLLIITPSHISHIQAAVICSKSHGLQIRTRSGGHDFEGL 120
           YSSVL+FSIRNL FSKPETPKPLLIITPSH+SHI+AAVICSKSHGLQIRTRSGGHD+EGL
Sbjct: 61  YSSVLNFSIRNLRFSKPETPKPLLIITPSHVSHIRAAVICSKSHGLQIRTRSGGHDYEGL 120

Query: 121 SYVAYRPFIVVDLINLRSVKVDVENNTAWVESGATLGELYYKIGEKSRTLAFPAGVCPTV 180
           SYVA  PFI++DLINLRS+K+DVE+NTAWVESG TLGELYY+IGEKSRTLAFPAG+CPTV
Sbjct: 121 SYVASHPFIILDLINLRSMKIDVESNTAWVESGTTLGELYYRIGEKSRTLAFPAGICPTV 180

Query: 181 GIGGHFSGGGYGLMLRKFGLAADNVIDAYLVDAHGKVLDRESMGEDLFWAIRGGGGGSFG 240
           G+GGHFSGGGYGLMLRKFGLAADNVIDAYLVDA+GKV DRESMGEDLFWAIRGGGGGSFG
Sbjct: 181 GVGGHFSGGGYGLMLRKFGLAADNVIDAYLVDANGKVHDRESMGEDLFWAIRGGGGGSFG 240

Query: 241 IVVAWKIKLVRVPPTVTICSTDRNLEEDTIRLIHRWQYVGNKLDENVYLGIILTGGN-AS 300
           IVVAWKIKLV VP TVT+C+T RNL+ D I+L+H+WQYV NKLDEN++LGIILTGGN  +
Sbjct: 241 IVVAWKIKLVSVPATVTMCNTYRNLKGDGIKLVHQWQYVANKLDENLFLGIILTGGNITT 300

Query: 301 TQAGITNPTARFFSLFLGRVDEFMATLSTTFPELGLIKQDCVEASWVESTLIIPIGVQPI 360
           TQ GITNP A FFSLFLG+V+E +ATLSTTFPELGLIKQDC+E SW+ESTLI   GVQ +
Sbjct: 301 TQEGITNPIATFFSLFLGKVNELVATLSTTFPELGLIKQDCIETSWIESTLIASTGVQTV 360

Query: 361 ESLEPLLNRTPTYLDSTKIKSDYVKEPISEATIEGIWQRLKAQDIETSQVIFVPYGGRMS 420
           ESLEPLLNRTP+ L++ KIKSDY+KEPIS ATIEGIWQRLKAQDIETSQ+IF+PYGGRMS
Sbjct: 361 ESLEPLLNRTPSNLENEKIKSDYIKEPISIATIEGIWQRLKAQDIETSQLIFIPYGGRMS 420

Query: 421 QISESETPFPHRAGYLFKIAYVVGWKDQSLKAKKTHISWIREIYEYMAPFVSKSPRAAYT 480
           QISESETPF HR G L+KI Y++GWK+QSLKAKK HISWIREIYEYM PFVSKSPR+AY 
Sbjct: 421 QISESETPFSHRVGNLYKIGYILGWKEQSLKAKKKHISWIREIYEYMTPFVSKSPRSAYA 480

Query: 481 NYRDLDIGSNNKYGKTSYKRASIWGMKYFGDNFDRLVYVKTKVDPYDFFRHEQSIPTL 538
           NYRDLDIG N KYGKTS K+ASIWG+KYFG+NF RLVYVKTKVDPYDFFRHEQSIPTL
Sbjct: 481 NYRDLDIGVNKKYGKTSVKQASIWGLKYFGNNFKRLVYVKTKVDPYDFFRHEQSIPTL 537

BLAST of CSPI01G25140 vs. NCBI nr
Match: gi|659068685|ref|XP_008445674.1| (PREDICTED: tetrahydrocannabinolic acid synthase-like [Cucumis melo])

HSP 1 Score: 902.1 bits (2330), Expect = 4.6e-259
Identity = 439/538 (81.60%), Postives = 485/538 (90.15%), Query Frame = 1

Query: 1   MKFSSTLIPLSLIFIIVVSPASLCASASPQKYQNFLHCLSEHSSKSYPISKVVYTPINSS 60
           MK+SS LIP SLI +I+VS +S   SASP K+QNFL CLSEHSSK YPISKVV+TPINSS
Sbjct: 1   MKYSSPLIPFSLI-LILVSSSSSWVSASPHKHQNFLQCLSEHSSKRYPISKVVHTPINSS 60

Query: 61  YSSVLDFSIRNLLFSKPETPKPLLIITPSHISHIQAAVICSKSHGLQIRTRSGGHDFEGL 120
           YSSVLDFSIRNLLFSKPETPKPLLIITPSH+SHIQAAVICSKSHGLQIRTRSGGHDFEGL
Sbjct: 61  YSSVLDFSIRNLLFSKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGL 120

Query: 121 SYVAYRPFIVVDLINLRSVKVDVENNTAWVESGATLGELYYKIGEKSRTLAFPAGVCPTV 180
           SYVAY PFI+VDLINLRS+K+DVE NTAWVESGATLGELYY+IGEKSRTLAFP GVCPTV
Sbjct: 121 SYVAYHPFIIVDLINLRSIKIDVETNTAWVESGATLGELYYRIGEKSRTLAFPGGVCPTV 180

Query: 181 GIGGHFSGGGYGLMLRKFGLAADNVIDAYLVDAHGKVLDRESMGEDLFWAIRGGGGGSFG 240
           G+GGHFSGGGYGLMLRKFGLAADNVIDAYLVDA+GKV DR+SMGEDLFWAIRGGGGGSFG
Sbjct: 181 GVGGHFSGGGYGLMLRKFGLAADNVIDAYLVDANGKVHDRKSMGEDLFWAIRGGGGGSFG 240

Query: 241 IVVAWKIKLVRVPPTVTICSTDRNLEEDTIRLIHRWQYVGNKLDENVYLGIILTGGN-AS 300
           IVVAWKIKLV VP TVTICST+RNL+ D I+L+H+WQYV NKLDEN++LGI+LTGGN  +
Sbjct: 241 IVVAWKIKLVSVPATVTICSTNRNLKGDGIKLVHQWQYVANKLDENLFLGIMLTGGNITT 300

Query: 301 TQAGITNPTARFFSLFLGRVDEFMATLSTTFPELGLIKQDCVEASWVESTLIIPIGVQPI 360
           TQ GI NP A FFSLFLG+VD  +ATLS  FPELG  K+DCVE SW+ESTLI   GV  +
Sbjct: 301 TQEGIANPIATFFSLFLGKVDILVATLSAIFPELGFTKEDCVETSWIESTLIAATGVGTV 360

Query: 361 ESLEPLLNRTPTYLDSTKIKSDYVKEPISEATIEGIWQRLKAQDIETSQVIFVPYGGRMS 420
           ESLEPLLNRTP+ L++ K+KSDY KEPIS ATIEG+WQRLKA+DIETSQ+IF+PYGGRMS
Sbjct: 361 ESLEPLLNRTPSNLENGKMKSDYFKEPISIATIEGLWQRLKAKDIETSQLIFIPYGGRMS 420

Query: 421 QISESETPFPHRAGYLFKIAYVVGWKDQSLKAKKTHISWIREIYEYMAPFVSKSPRAAYT 480
           QISE ETPF HRAG L+KI Y V WK+QSLKAKK HISWIR+IY+YM PFVSKSPR+AY 
Sbjct: 421 QISEMETPFSHRAGILYKIGYFVAWKEQSLKAKKKHISWIRDIYKYMTPFVSKSPRSAYA 480

Query: 481 NYRDLDIGSNNKYGKTSYKRASIWGMKYFGDNFDRLVYVKTKVDPYDFFRHEQSIPTL 538
           NYRDLDIG+N KYGKTS ++ASIWG+KYFG+NF RLVYVKTKVDPYDFFRHEQSIPTL
Sbjct: 481 NYRDLDIGANKKYGKTSVRQASIWGLKYFGNNFKRLVYVKTKVDPYDFFRHEQSIPTL 537

BLAST of CSPI01G25140 vs. NCBI nr
Match: gi|778662000|ref|XP_011659198.1| (PREDICTED: cannabidiolic acid synthase-like 1 [Cucumis sativus])

HSP 1 Score: 894.0 bits (2309), Expect = 1.3e-256
Identity = 434/542 (80.07%), Postives = 482/542 (88.93%), Query Frame = 1

Query: 1   MKFSSTLIPLSLIFIIVVSPASLCASASPQKYQNFLHCLSEHSSKSYPISKVVYTPINSS 60
           MK SS LIPL L+  IV+S +    SA P K+QNF+ CLSEHSSKSYPISKVVYTPINSS
Sbjct: 1   MKSSSQLIPLFLVLTIVLSASPSWVSAFPHKHQNFVQCLSEHSSKSYPISKVVYTPINSS 60

Query: 61  YSSVLDFSIRNLLFSKPETPKPLLIITPSHISHIQAAVICSKSHGLQIRTRSGGHDFEGL 120
           YSSVL+FSIRNL FSKPETPKPLLIITPSH+SHIQAAVICSKSH LQIRTRSGGHD+EGL
Sbjct: 61  YSSVLNFSIRNLRFSKPETPKPLLIITPSHVSHIQAAVICSKSHDLQIRTRSGGHDYEGL 120

Query: 121 SYVAYRPFIVVDLINLRSVKVDVENNTAWVESGATLGELYYKIGEKSRTLAFPAGVCPTV 180
           SYVAY PFI+VDLINLRS+ +D+ NNTAWVESGATLGELYY+IGEKSRTLAFPAG+CPTV
Sbjct: 121 SYVAYHPFIIVDLINLRSISIDIVNNTAWVESGATLGELYYRIGEKSRTLAFPAGICPTV 180

Query: 181 GIGGHFSGGGYGLMLRKFGLAADNVIDAYLVDAHGKVLDRESMGEDLFWAIRGGGGGSFG 240
           G+GGHFSGGGYG +LRK+GLAADNVIDAYLVDA+G V DRESMGEDLFWAIRGG GGSFG
Sbjct: 181 GVGGHFSGGGYGFLLRKYGLAADNVIDAYLVDANGMVHDRESMGEDLFWAIRGGSGGSFG 240

Query: 241 IVVAWKIKLVRVPPTVTICSTDRNLEEDTIRLIHRWQYVGNKLDENVYLGIILTGGNAST 300
           IVVAWK+KLV VP  VTICST +NLE+D +++IH+WQYV NKL E+++LGI+LTGGN ST
Sbjct: 241 IVVAWKVKLVPVPAMVTICSTIKNLEDDAVKVIHQWQYVANKLHEDIFLGIVLTGGNTST 300

Query: 301 QAG----ITNPTARFFSLFLGRVDEFMATLSTTFPELGLIKQDCVEASWVESTLIIPIGV 360
           Q G    I NP A F+SLFLG+VDE +ATLST FPELGLIKQDC+E  WVESTLII  GV
Sbjct: 301 QGGIKNPIKNPIATFYSLFLGKVDELVATLSTKFPELGLIKQDCLEVRWVESTLIIASGV 360

Query: 361 -QPIESLEPLLNRTPTYLDSTKIKSDYVKEPISEATIEGIWQRLKAQDIETSQVIFVPYG 420
            Q IESLEPLLNRTPT LDSTKIKSDY+K+PI +A IEGIWQRLKA+DIE  Q +F PYG
Sbjct: 361 FQTIESLEPLLNRTPTTLDSTKIKSDYIKKPIPKAAIEGIWQRLKARDIEGPQFVFAPYG 420

Query: 421 GRMSQISESETPFPHRAGYLFKIAYVVGWKDQSLKAKKTHISWIREIYEYMAPFVSKSPR 480
            RMSQIS+SETPF HRAGYL++I Y+VGWK Q+LKAK  HISWIRE+YEYMAPFVSKSPR
Sbjct: 421 ARMSQISKSETPFSHRAGYLYQIGYMVGWKGQNLKAKNRHISWIRELYEYMAPFVSKSPR 480

Query: 481 AAYTNYRDLDIGSNNKYGKTSYKRASIWGMKYFGDNFDRLVYVKTKVDPYDFFRHEQSIP 538
           AAYTNYRDLDIGSNNKYGKTSYK+ASIWG+KYFG+NF RLVYVKTKVDP+DFFRHEQSIP
Sbjct: 481 AAYTNYRDLDIGSNNKYGKTSYKQASIWGLKYFGNNFKRLVYVKTKVDPHDFFRHEQSIP 540

BLAST of CSPI01G25140 vs. NCBI nr
Match: gi|659068690|ref|XP_008445695.1| (PREDICTED: tetrahydrocannabinolic acid synthase-like [Cucumis melo])

HSP 1 Score: 890.6 bits (2300), Expect = 1.4e-255
Identity = 427/539 (79.22%), Postives = 479/539 (88.87%), Query Frame = 1

Query: 1   MKFSSTLIPL-SLIFIIVVSPASLCASASPQKYQNFLHCLSEHSSKSYPISKVVYTPINS 60
           MK SS L PL  ++ I+VVS + L  SA P ++QNFLHCL EHSSKSYPISKVVYTPINS
Sbjct: 1   MKSSSQLTPLFHVLIIVVVSSSPLWVSAFPHEHQNFLHCLPEHSSKSYPISKVVYTPINS 60

Query: 61  SYSSVLDFSIRNLLFSKPETPKPLLIITPSHISHIQAAVICSKSHGLQIRTRSGGHDFEG 120
           SYSSVL+FSIRN  FSKPETPKPLLIITPSH+SHIQAAVICSKSHGLQIRTRSGGHD+EG
Sbjct: 61  SYSSVLNFSIRNFRFSKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDYEG 120

Query: 121 LSYVAYRPFIVVDLINLRSVKVDVENNTAWVESGATLGELYYKIGEKSRTLAFPAGVCPT 180
           LSYVAYRPFI+VDLIN+RS+ VD+ NNTAWVESGATLGELYY+IGEKSRTLAFPAG+CPT
Sbjct: 121 LSYVAYRPFIIVDLINIRSISVDIVNNTAWVESGATLGELYYRIGEKSRTLAFPAGICPT 180

Query: 181 VGIGGHFSGGGYGLMLRKFGLAADNVIDAYLVDAHGKVLDRESMGEDLFWAIRGGGGGSF 240
           VG+GGHFSGGGYG +LRK+GLAADNVIDAYLVDA+G   DRESMGEDLFWAIRGGGGGSF
Sbjct: 181 VGVGGHFSGGGYGFLLRKYGLAADNVIDAYLVDANGMAHDRESMGEDLFWAIRGGGGGSF 240

Query: 241 GIVVAWKIKLVRVPPTVTICSTDRNLEEDTIRLIHRWQYVGNKLDENVYLGIILTGGNAS 300
           GIVVAWK+KLV VP  VTICST +NLE D +++IH+WQYV NKL E+++LGI+LTGGN S
Sbjct: 241 GIVVAWKVKLVPVPALVTICSTIKNLEGDAVKVIHQWQYVANKLHEDIFLGIVLTGGNTS 300

Query: 301 TQAGITNPTARFFSLFLGRVDEFMATLSTTFPELGLIKQDCVEASWVESTLIIPIGV-QP 360
           TQ GI  PTA F+SLFLG+VDE +ATLSTTFPE GLIK+DC+E  W+ESTLII  GV Q 
Sbjct: 301 TQGGIKKPTATFYSLFLGKVDELVATLSTTFPEFGLIKKDCLEVRWIESTLIIASGVFQT 360

Query: 361 IESLEPLLNRTPTYLDSTKIKSDYVKEPISEATIEGIWQRLKAQDIETSQVIFVPYGGRM 420
           +ESL+PLLNRTP+ +DSTKIKSDY+K+PI +A IEGIWQRLKA DIE  Q++FVPYG RM
Sbjct: 361 VESLKPLLNRTPSTIDSTKIKSDYIKKPIPKAAIEGIWQRLKAGDIEAPQLVFVPYGARM 420

Query: 421 SQISESETPFPHRAGYLFKIAYVVGWKDQSLKAKKTHISWIREIYEYMAPFVSKSPRAAY 480
           SQISESETPF HRAGYL+KI Y+VGWK Q+LKAK  HISW RE+YEYM PFVSKSPR AY
Sbjct: 421 SQISESETPFSHRAGYLYKIGYMVGWKGQNLKAKNRHISWTRELYEYMTPFVSKSPRTAY 480

Query: 481 TNYRDLDIGSNNKYGKTSYKRASIWGMKYFGDNFDRLVYVKTKVDPYDFFRHEQSIPTL 538
            NYRDLDIGSNNKYGKTSYK+ASIWG+KYFG+NF RLVYVK KVDP+DFFRHEQSIPTL
Sbjct: 481 VNYRDLDIGSNNKYGKTSYKQASIWGLKYFGNNFKRLVYVKNKVDPHDFFRHEQSIPTL 539