BLAST of Bhi08G000824 vs. Swiss-Prot
Match:
sp|Q8GTB6|THCAS_CANSA (Tetrahydrocannabinolic acid synthase OS=Cannabis sativa OX=3483 PE=1 SV=1)
HSP 1 Score: 540.8 bits (1392), Expect = 1.7e-152
Identity = 263/509 (51.67%), Postives = 354/509 (69.55%), Query Frame = 0
Query: 33 ENFLECLFHHSPN---ISKVIYTPFNSSYSSILNFTLRNLRFSTPNTPKPLLIITPSHIS 92
ENFL+C H PN K++YT + Y SILN T++NLRF + TPKPL+I+TPS+ S
Sbjct: 32 ENFLKCFSKHIPNNVANPKLVYTQHDQLYMSILNSTIQNLRFISDTTPKPLVIVTPSNNS 91
Query: 93 HIQVAVICSKSYGLQIRTRSGGHDFEGLSYVAYLPFIIVDLINLSSIQIEIETNTAWVES 152
HIQ ++CSK GLQIRTRSGGHD EG+SY++ +PF++VDL N+ SI+I++ + TAWVE+
Sbjct: 92 HIQATILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVVVDLRNMHSIKIDVHSQTAWVEA 151
Query: 153 GATLGELYYRIAEKSRTLAFPAGTCPIVGVGGHLSGGGYGMLMRKYGLAADNVIDAYLVD 212
GATLGE+YY I EK+ L+FP G CP VGVGGH SGGGYG LMR YGLAADN+IDA+LV+
Sbjct: 152 GATLGEVYYWINEKNENLSFPGGYCPTVGVGGHFSGGGYGALMRNYGLAADNIIDAHLVN 211
Query: 213 ANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVLVPATVTLCFVSKTWE-EGATN 272
+G+ DR+SMGEDLFWAIRGGGG +FGI+ AWK+KLV VP+ T+ V K E G
Sbjct: 212 VDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIKLVAVPSKSTIFSVKKNMEIHGLVK 271
Query: 273 LIHRWQYVAHDLDENLFIGILLSGGNFTRQEGKTNPT--ALFFSLYLGKVDELVSILNKT 332
L ++WQ +A+ D++L + N T GK T F S++ G VD LV ++NK+
Sbjct: 272 LFNKWQNIAYKYDKDLVLMTHFITKNITDNHGKNKTTVHGYFSSIFHGGVDSLVDLMNKS 331
Query: 333 FPELGLAKKDCIETSWVESALIMGTGLQ---PGKSLEDLLSKASLTNGNTKIKSDYIKQP 392
FPELG+ K DC E SW+++ + + E LL +++ IK DY+K+P
Sbjct: 332 FPELGIKKTDCKEFSWIDTTIFYSGVVNFNTANFKKEILLDRSAGKKTAFSIKLDYVKKP 391
Query: 393 IPLAAVEGIWQRLKLEDIEMPQLLFAPYGGRMSQISESESPFSHRAGYLYKIGYVTYWEQ 452
IP A+ I ++L ED+ + PYGG M +ISES PF HRAG +Y++ Y WE+
Sbjct: 392 IPETAMVKILEKLYEEDVGAGMYVLYPYGGIMEEISESAIPFPHRAGIMYELWYTASWEK 451
Query: 453 QDNDVEKRHLSWIRELYSYMTPFVSKSPRAAYINYRDLDIGSNNKYGKTSYKQASIWGLK 512
Q+++ ++H++W+R +Y++ TP+VS++PR AY+NYRDLD+G N +Y QA IWG K
Sbjct: 452 QEDN--EKHINWVRSVYNFTTPYVSQNPRLAYLNYRDLDLGKTNHASPNNYTQARIWGEK 511
Query: 513 YFGNNFNRLVDVKTRVDPHNFFRHEQSIP 533
YFG NFNRLV VKT+VDP+NFFR+EQSIP
Sbjct: 512 YFGKNFNRLVKVKTKVDPNNFFRNEQSIP 538
BLAST of Bhi08G000824 vs. Swiss-Prot
Match:
sp|A6P6V9|CBDAS_CANSA (Cannabidiolic acid synthase OS=Cannabis sativa OX=3483 GN=CBDAS PE=1 SV=1)
HSP 1 Score: 539.7 bits (1389), Expect = 3.8e-152
Identity = 269/520 (51.73%), Postives = 368/520 (70.77%), Query Frame = 0
Query: 24 VASNSSEKHENFLECLFHHSPNIS---KVIYTPFNSSYSSILNFTLRNLRFSTPNTPKPL 83
+ ++ + ENFL+C + PN + K++YT N Y S+LN T+ NLRF++ TPKPL
Sbjct: 23 IQTSIANPRENFLKCFSQYIPNNATNLKLVYTQNNPLYMSVLNSTIHNLRFTSDTTPKPL 82
Query: 84 LIITPSHISHIQVAVICSKSYGLQIRTRSGGHDFEGLSYVAYLPFIIVDLINLSSIQIEI 143
+I+TPSH+SHIQ ++CSK GLQIRTRSGGHD EG+SY++ +PF+IVDL N+ SI+I++
Sbjct: 83 VIVTPSHVSHIQGTILCSKKVGLQIRTRSGGHDSEGMSYISQVPFVIVDLRNMRSIKIDV 142
Query: 144 ETNTAWVESGATLGELYYRIAEKSRTLAFPAGTCPIVGVGGHLSGGGYGMLMRKYGLAAD 203
+ TAWVE+GATLGE+YY + EK+ L+ AG CP V GGH GGGYG LMR YGLAAD
Sbjct: 143 HSQTAWVEAGATLGEVYYWVNEKNENLSLAAGYCPTVCAGGHFGGGGYGPLMRNYGLAAD 202
Query: 204 NVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVLVPATVTLCFVSK 263
N+IDA+LV+ +G+ DR+SMGEDLFWA+RGGG SFGI+VAWK++LV VP + T+ V K
Sbjct: 203 NIIDAHLVNVHGKVLDRKSMGEDLFWALRGGGAESFGIIVAWKIRLVAVPKS-TMFSVKK 262
Query: 264 TWE-EGATNLIHRWQYVAHDLDENLFIGILLSGGNFTRQEGKTNPTAL---FFSLYLGKV 323
E L+++WQ +A+ D++L + N T +GK N TA+ F S++LG V
Sbjct: 263 IMEIHELVKLVNKWQNIAYKYDKDLLLMTHFITRNITDNQGK-NKTAIHTYFSSVFLGGV 322
Query: 324 DELVSILNKTFPELGLAKKDCIETSWVESALIMGTGL----QPGKSLEDLLSKASLTNGN 383
D LV ++NK+FPELG+ K DC + SW+++ +I +G+ + E LL +++ NG
Sbjct: 323 DSLVDLMNKSFPELGIKKTDCRQLSWIDT-IIFYSGVVNYDTDNFNKEILLDRSAGQNGA 382
Query: 384 TKIKSDYIKQPIPLAAVEGIWQRLKLEDIEMPQLLFAPYGGRMSQISESESPFSHRAGYL 443
KIK DY+K+PIP + I ++L EDI PYGG M +ISES PF HRAG L
Sbjct: 383 FKIKLDYVKKPIPESVFVQILEKLYEEDIGAGMYALYPYGGIMDEISESAIPFPHRAGIL 442
Query: 444 YKIGYVTYWEQQDNDVEKRHLSWIRELYSYMTPFVSKSPRAAYINYRDLDIGSNNKYGKT 503
Y++ Y+ WE+Q+++ ++HL+WIR +Y++MTP+VSK+PR AY+NYRDLDIG N+
Sbjct: 443 YELWYICSWEKQEDN--EKHLNWIRNIYNFMTPYVSKNPRLAYLNYRDLDIGINDPKNPN 502
Query: 504 SYKQASIWGLKYFGNNFNRLVDVKTRVDPHNFFRHEQSIP 533
+Y QA IWG KYFG NF+RLV VKT VDP+NFFR+EQSIP
Sbjct: 503 NYTQARIWGEKYFGKNFDRLVKVKTLVDPNNFFRNEQSIP 537
BLAST of Bhi08G000824 vs. Swiss-Prot
Match:
sp|A6P6W0|CASL1_CANSA (Cannabidiolic acid synthase-like 1 OS=Cannabis sativa OX=3483 GN=CBDAS2 PE=2 SV=1)
HSP 1 Score: 536.2 bits (1380), Expect = 4.2e-151
Identity = 260/509 (51.08%), Postives = 359/509 (70.53%), Query Frame = 0
Query: 33 ENFLECLFHHSP-NI--SKVIYTPFNSSYSSILNFTLRNLRFSTPNTPKPLLIITPSHIS 92
ENFL+C + P N+ +K++YT + Y SILN T++NLRF++ TPKPL+IITP ++S
Sbjct: 32 ENFLKCFSQYIPTNVTNAKLVYTQHDQFYMSILNSTIQNLRFTSDTTPKPLVIITPLNVS 91
Query: 93 HIQVAVICSKSYGLQIRTRSGGHDFEGLSYVAYLPFIIVDLINLSSIQIEIETNTAWVES 152
HIQ ++CSK GLQIRTRSGGHD EG+SY++ +PF+IVDL N+ S++I++ + TAWVE+
Sbjct: 92 HIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLRNMHSVKIDVHSQTAWVEA 151
Query: 153 GATLGELYYRIAEKSRTLAFPAGTCPIVGVGGHLSGGGYGMLMRKYGLAADNVIDAYLVD 212
GATLGE+YY I E + L+FPAG CP VG GGH SGGGYG LMR YGLAADN+IDA+LV+
Sbjct: 152 GATLGEVYYWINENNENLSFPAGYCPTVGAGGHFSGGGYGALMRNYGLAADNIIDAHLVN 211
Query: 213 ANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVLVPATVTLCFVSKTWE-EGATN 272
+G+ DR+SMGEDLFWAIRGGGG +FGI+ AWK++LV VP+ T+ V K E
Sbjct: 212 VDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVPSMSTIFSVKKNMEIHELVK 271
Query: 273 LIHRWQYVAHDLDENLFIGILLSGGNFTRQEGKTNPT--ALFFSLYLGKVDELVSILNKT 332
L+++WQ +A+ ++ L + N T +GK T + F S++ G VD LV ++NK+
Sbjct: 272 LVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTTIHSYFSSIFHGGVDSLVDLMNKS 331
Query: 333 FPELGLAKKDCIETSWVESALIMGTGLQPGKSL---EDLLSKASLTNGNTKIKSDYIKQP 392
FPELG+ K DC + SW+++ + + + E LL ++ IK DY+K+P
Sbjct: 332 FPELGIKKTDCKQLSWIDTIIFYSGVVNYNTTYFKKEILLDRSGGRKAAFSIKLDYVKKP 391
Query: 393 IPLAAVEGIWQRLKLEDIEMPQLLFAPYGGRMSQISESESPFSHRAGYLYKIGYVTYWEQ 452
IP A+ I ++L ED+ + +F PYGG M +ISES PF HRAG +Y+I Y+ WE+
Sbjct: 392 IPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISESAIPFPHRAGIMYEIWYIASWEK 451
Query: 453 QDNDVEKRHLSWIRELYSYMTPFVSKSPRAAYINYRDLDIGSNNKYGKTSYKQASIWGLK 512
Q+++ ++H++WIR +Y++ TP+VS++PR AY+NYRDLD+G N +Y QA IWG K
Sbjct: 452 QEDN--EKHINWIRNVYNFTTPYVSQNPRMAYLNYRDLDLGKTNFESPNNYTQARIWGEK 511
Query: 513 YFGNNFNRLVDVKTRVDPHNFFRHEQSIP 533
YFG NFNRLV VKT+VDP NFFR+EQSIP
Sbjct: 512 YFGKNFNRLVKVKTKVDPDNFFRNEQSIP 538
BLAST of Bhi08G000824 vs. Swiss-Prot
Match:
sp|Q33DQ2|THCAI_CANSA (Inactive tetrahydrocannabinolic acid synthase OS=Cannabis sativa OX=3483 PE=3 SV=1)
HSP 1 Score: 536.2 bits (1380), Expect = 4.2e-151
Identity = 258/509 (50.69%), Postives = 355/509 (69.74%), Query Frame = 0
Query: 33 ENFLECLFHHSPN---ISKVIYTPFNSSYSSILNFTLRNLRFSTPNTPKPLLIITPSHIS 92
ENFL+C + PN K IYT + Y S+LN T++NLRF++ TPKPL+I+TPS++S
Sbjct: 32 ENFLKCFSEYIPNNPANPKFIYTQHDQLYMSVLNSTIQNLRFTSDTTPKPLVIVTPSNVS 91
Query: 93 HIQVAVICSKSYGLQIRTRSGGHDFEGLSYVAYLPFIIVDLINLSSIQIEIETNTAWVES 152
HIQ +++CSK GLQIRTRSGGHD EGLSY++ +PF IVDL N+ +++++I + TAWVE+
Sbjct: 92 HIQASILCSKKVGLQIRTRSGGHDAEGLSYISQVPFAIVDLRNMHTVKVDIHSQTAWVEA 151
Query: 153 GATLGELYYRIAEKSRTLAFPAGTCPIVGVGGHLSGGGYGMLMRKYGLAADNVIDAYLVD 212
GATLGE+YY I E + +FP G CP VGVGGH SGGGYG LMR YGLAADN+IDA+LV+
Sbjct: 152 GATLGEVYYWINEMNENFSFPGGYCPTVGVGGHFSGGGYGALMRNYGLAADNIIDAHLVN 211
Query: 213 ANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVLVPATVTLCFVSKTWE-EGATN 272
+G+ DR+SMGEDLFWAIRGGGG +FGI+ AWK+KLV+VP+ T+ V K E G
Sbjct: 212 VDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIKLVVVPSKATIFSVKKNMEIHGLVK 271
Query: 273 LIHRWQYVAHDLDENLFIGILLSGGNFTRQEGKTNPT--ALFFSLYLGKVDELVSILNKT 332
L ++WQ +A+ D++L + N T GK T F S++LG VD LV ++NK+
Sbjct: 272 LFNKWQNIAYKYDKDLMLTTHFRTRNITDNHGKNKTTVHGYFSSIFLGGVDSLVDLMNKS 331
Query: 333 FPELGLAKKDCIETSWVESALIMGTGLQPGKS---LEDLLSKASLTNGNTKIKSDYIKQP 392
FPELG+ K DC E SW+++ + + + E LL +++ IK DY+K+
Sbjct: 332 FPELGIKKTDCKELSWIDTTIFYSGVVNYNTANFKKEILLDRSAGKKTAFSIKLDYVKKL 391
Query: 393 IPLAAVEGIWQRLKLEDIEMPQLLFAPYGGRMSQISESESPFSHRAGYLYKIGYVTYWEQ 452
IP A+ I ++L E++ + + PYGG M +ISES PF HRAG +Y++ Y WE+
Sbjct: 392 IPETAMVKILEKLYEEEVGVGMYVLYPYGGIMDEISESAIPFPHRAGIMYELWYTATWEK 451
Query: 453 QDNDVEKRHLSWIRELYSYMTPFVSKSPRAAYINYRDLDIGSNNKYGKTSYKQASIWGLK 512
Q+++ ++H++W+R +Y++ TP+VS++PR AY+NYRDLD+G N +Y QA IWG K
Sbjct: 452 QEDN--EKHINWVRSVYNFTTPYVSQNPRLAYLNYRDLDLGKTNPESPNNYTQARIWGEK 511
Query: 513 YFGNNFNRLVDVKTRVDPHNFFRHEQSIP 533
YFG NFNRLV VKT+ DP+NFFR+EQSIP
Sbjct: 512 YFGKNFNRLVKVKTKADPNNFFRNEQSIP 538
BLAST of Bhi08G000824 vs. Swiss-Prot
Match:
sp|A6P6W1|CASL2_CANSA (Cannabidiolic acid synthase-like 2 OS=Cannabis sativa OX=3483 GN=CBDAS3 PE=2 SV=1)
HSP 1 Score: 535.0 bits (1377), Expect = 9.4e-151
Identity = 263/510 (51.57%), Postives = 360/510 (70.59%), Query Frame = 0
Query: 33 ENFLECLFHHSP-NI--SKVIYTPFNSSYSSILNFTLRNLRFSTPNTPKPLLIITPSHIS 92
ENFL+CL + P N+ +K++YT + Y SILN T++NLRF++ TPKPL+I TP ++S
Sbjct: 32 ENFLKCLSQYIPTNVTNAKLVYTQHDQFYMSILNSTVQNLRFTSDTTPKPLVITTPLNVS 91
Query: 93 HIQVAVICSKSYGLQIRTRSGGHDFEGLSYVAYLPFIIVDLINLSSIQIEIETNTAWVES 152
HIQ ++CSK GLQIRTRSGGHD EG+SY++ +PF+IVDL N+ S++I++ + TAWVES
Sbjct: 92 HIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLRNMHSVKIDVHSQTAWVES 151
Query: 153 GATLGELYYRIAEKSRTLAFPAGTCPIVGVGGHLSGGGYGMLMRKYGLAADNVIDAYLVD 212
GATLGE+YY I E + L+FPAG CP VG GGH SGGGYG LMR YGLAADN+IDA+LV+
Sbjct: 152 GATLGEVYYWINENNENLSFPAGYCPTVGTGGHFSGGGYGALMRNYGLAADNIIDAHLVN 211
Query: 213 ANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVLVPATVTLCFVSKTWE-EGATN 272
+G+ DR+SMGEDLFWAIRGGGG +FGI+ AWK++LV VP+ T+ V K E
Sbjct: 212 VDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVPSMSTIFSVKKNMEIHELVK 271
Query: 273 LIHRWQYVAHDLDENLFIGILLSGGNFTRQEGKTNPT--ALFFSLYLGKVDELVSILNKT 332
L+++WQ +A+ ++ L + N T +GK T + F S++ G VD LV ++NK+
Sbjct: 272 LVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTTIHSYFSSIFHGGVDSLVDLMNKS 331
Query: 333 FPELGLAKKDCIETSWVESALIMGTGL----QPGKSLEDLLSKASLTNGNTKIKSDYIKQ 392
FPELG+ K DC + SW+++ +I +G+ E LL ++ IK DY+K+
Sbjct: 332 FPELGIKKTDCKQLSWIDT-IIFYSGVVNYNTTNFKKEILLDRSGGRKAAFSIKLDYVKK 391
Query: 393 PIPLAAVEGIWQRLKLEDIEMPQLLFAPYGGRMSQISESESPFSHRAGYLYKIGYVTYWE 452
PIP A+ I ++L ED+ + +F PYGG M +ISES PF HRAG Y+I Y+ WE
Sbjct: 392 PIPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISESAIPFPHRAGITYEIWYIASWE 451
Query: 453 QQDNDVEKRHLSWIRELYSYMTPFVSKSPRAAYINYRDLDIGSNNKYGKTSYKQASIWGL 512
+Q+++ ++H++WIR +Y++ TP+VS++PR AY+NYRDLD+G N +Y QA IWG
Sbjct: 452 KQEDN--EKHINWIRNVYNFTTPYVSQNPRMAYLNYRDLDLGKTNFESPNNYTQARIWGE 511
Query: 513 KYFGNNFNRLVDVKTRVDPHNFFRHEQSIP 533
KYFG NFNRLV VKT+VDP NFFR+EQSIP
Sbjct: 512 KYFGKNFNRLVKVKTKVDPDNFFRNEQSIP 538
BLAST of Bhi08G000824 vs. TAIR10
Match:
AT4G20820.1 (FAD-binding Berberine family protein)
HSP 1 Score: 526.2 bits (1354), Expect = 2.4e-149
Identity = 254/517 (49.13%), Postives = 360/517 (69.63%), Query Frame = 0
Query: 22 PWVASNSSEKHENFLECL---FHHSPNISKVIYTPFNSSYSSILNFTLRNLRFSTPNTPK 81
P S + +FL+CL + S +SKVI+TP ++S+SS+L +++N RFS P+ PK
Sbjct: 24 PHPVSANRSNQSSFLQCLSLQLNDSNIVSKVIHTPNDTSFSSVLASSIQNQRFSAPDVPK 83
Query: 82 PLLIITPSHISHIQVAVICSKSYGLQIRTRSGGHDFEGLSYVAYLPFIIVDLINLSSIQI 141
P+LI+TP S +Q AV C++ +G+ IRTRSGGHD+EGLSYV + PF+I+DL NL SI +
Sbjct: 84 PVLILTPVQPSDVQSAVKCARRFGIHIRTRSGGHDYEGLSYVTHKPFVILDLRNLRSITV 143
Query: 142 EIETNTAWVESGATLGELYYRIAEKSRTLAFPAGTCPIVGVGGHLSGGGYGMLMRKYGLA 201
+++ + WV++GAT+GELYY I +K+RTLAFPAG CP VGVGGH SGGGYG L+RK+GLA
Sbjct: 144 DVDNRSVWVQTGATIGELYYEIGKKNRTLAFPAGVCPTVGVGGHFSGGGYGTLLRKHGLA 203
Query: 202 ADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVLVPATVTLCFV 261
AD+VIDA +VDA G +R MGED FWAIRGGGG SF +V++WK+ L+ VP+TVT+ V
Sbjct: 204 ADHVIDARVVDARGRILERREMGEDFFWAIRGGGGSSFCVVLSWKIGLINVPSTVTVFNV 263
Query: 262 SKTWEEGATNLIHRWQYVAHDLDENLFIGILLSGGNFTRQEGKTNPTALFFSLYLGKVDE 321
+K E+ A +IHRWQ+VA + ++LFI ++L Q K A F LYLG V
Sbjct: 264 TKFSEQSALKIIHRWQFVADKVSDDLFIRVML-------QRYKNMVRASFPGLYLGSVKN 323
Query: 322 LVSILNKTFPELGLAKKDCIETSWVESALIMGTGLQPGKSLEDLLSKASLTNGNTKIKSD 381
L+ ++NK FPELGL + DC E SW+ES + + G+ ++L+K + + K KSD
Sbjct: 324 LLKMVNKEFPELGLEEDDCTEMSWIESVIWFA---ELGEEPINVLTKRTRASLAFKAKSD 383
Query: 382 YIKQPIPLAAVEGIWQRLKLEDIEMPQLLFAPYGGRMSQISESESPFSHRAGYLYKIGYV 441
++++P+P A+ +W+RL+ + E QL+F P+GG+MS+I++ E+PF HR G +Y+I Y+
Sbjct: 384 FVQEPMPKTAISKLWRRLQEPEAEHAQLIFTPFGGKMSEIADYETPFPHRKGNIYEIQYL 443
Query: 442 TYWEQQDNDVEKRHLSWIRELYSYMTPFVSKSPRAAYINYRDLDIGSNNKYGKTSYKQAS 501
YW DV+++++ W+ +Y M+ FV+KSPR AYIN RDLD+G ++ Y++
Sbjct: 444 NYWR---GDVKEKYMRWVERVYDDMSEFVAKSPRGAYINLRDLDLGMYVGVKRSKYEEGK 503
Query: 502 IWGLKYFGNNFNRLVDVKTRVDPHNFFRHEQSIPTFS 536
WG+KYF NNF RLV VKT VDP +FF EQSIP F+
Sbjct: 504 SWGVKYFKNNFERLVRVKTSVDPSDFFCDEQSIPPFT 527
BLAST of Bhi08G000824 vs. TAIR10
Match:
AT1G30700.1 (FAD-binding Berberine family protein)
HSP 1 Score: 525.0 bits (1351), Expect = 5.4e-149
Identity = 263/516 (50.97%), Postives = 356/516 (68.99%), Query Frame = 0
Query: 23 WVASNSSEKHENFLECLFHHSP---NISKVIYTPFNSSYSSILNFTLRNLRFSTPNTPKP 82
W +S+SS E F +CL +S IS I+ N SYSS+L +RNLRF+T +TPKP
Sbjct: 16 WQSSSSSANSETFTQCLTSNSDPKHPISPAIFFSGNGSYSSVLQANIRNLRFNTTSTPKP 75
Query: 83 LLIITPSHISHIQVAVICSKSYGLQIRTRSGGHDFEGLSYVAY--LPFIIVDLINLSSIQ 142
LII +H SH+Q A+ C K + LQ++ RSGGHD++GLSYV Y PF ++D+ NL S+
Sbjct: 76 FLIIAATHESHVQAAITCGKRHNLQMKIRSGGHDYDGLSYVTYSGKPFFVLDMFNLRSVD 135
Query: 143 IEIETNTAWVESGATLGELYYRIAEKSRTLAFPAGTCPIVGVGGHLSGGGYGMLMRKYGL 202
+++ + TAWV++GA LGE+YY I EKS+TLA+PAG CP VGVGGH+SGGGYG +MRKYGL
Sbjct: 136 VDVASKTAWVQTGAILGEVYYYIWEKSKTLAYPAGICPTVGVGGHISGGGYGNMMRKYGL 195
Query: 203 AADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVLVPATVTLCF 262
DN IDA +VD NG+ DR+ MGEDL+WAI GGGGGS+G+V+A+K+ LV VP VT+
Sbjct: 196 TVDNTIDARMVDVNGKILDRKLMGEDLYWAINGGGGGSYGVVLAYKINLVEVPENVTVFR 255
Query: 263 VSKTWEEGATNLIHRWQYVAHDLDENLFIGILLSGGNFTRQEGKTNPTALFFSLYLGKVD 322
+S+T E+ AT++IHRWQ VA L + LFI ++ N T KT T F +++LG
Sbjct: 256 ISRTLEQNATDIIHRWQQVAPKLPDELFIRTVIDVVNGTVSSQKTVRTT-FIAMFLGDTT 315
Query: 323 ELVSILNKTFPELGLAKKDCIETSWVESALIMGTGLQPGKSLEDLLSKASLTNGNTKIKS 382
L+SILN+ FPELGL + DC ETSW++S L T +Q G S E LL + + K KS
Sbjct: 316 TLLSILNRRFPELGLVRSDCTETSWIQSVLFW-TNIQVGSS-ETLLLQRNQPVNYLKRKS 375
Query: 383 DYIKQPIPLAAVEGIWQRLKLEDIEMPQLLFAPYGGRMSQISESESPFSHRAGYLYKIGY 442
DY+++PI +E IW+ K+ ++E+P + F PYGG M +IS + +PF +RAG L+KI Y
Sbjct: 376 DYVREPISRTGLESIWK--KMIELEIPTMAFNPYGGEMGRISSTVTPFPYRAGNLWKIQY 435
Query: 443 VTYWEQQDNDVEKRHLSWIRELYSYMTPFVSKSPRAAYINYRDLDIGSNNKYGK-TSYKQ 502
W +D + R++ R+LY +MTPFVSK+PR ++ NYRD+D+G N+ GK +SY +
Sbjct: 436 GANW--RDETLTDRYMELTRKLYQFMTPFVSKNPRQSFFNYRDVDLGINSHNGKISSYVE 495
Query: 503 ASIWGLKYFGNNFNRLVDVKTRVDPHNFFRHEQSIP 533
+G KYF NF RLV +KTRVD NFFR+EQSIP
Sbjct: 496 GKRYGKKYFAGNFERLVKIKTRVDSGNFFRNEQSIP 524
BLAST of Bhi08G000824 vs. TAIR10
Match:
AT2G34790.1 (FAD-binding Berberine family protein)
HSP 1 Score: 517.7 bits (1332), Expect = 8.7e-147
Identity = 252/518 (48.65%), Postives = 361/518 (69.69%), Query Frame = 0
Query: 24 VASNSSEKHENFLECLFHHSP---NISKVIYTPFNSS--YSSILNFTLRNLRFSTPNTPK 83
V +SS ++F++CL +S I+ ++P ++ + L T +NLR+ TP+ PK
Sbjct: 22 VPLSSSTLQQDFVKCLVDNSDVSFPITASFFSPDQNATLFKEELESTAQNLRYLTPSNPK 81
Query: 84 PLLIITPSHISHIQVAVICSKSYGLQIRTRSGGHDFEGLSYVA--YLPFIIVDLINLSSI 143
P+ I P + +H+Q AV+C+K L +R RSGGHD+EGLS+VA PF+IVDL L +
Sbjct: 82 PVFIFEPLYETHVQAAVVCAKKLQLHLRLRSGGHDYEGLSFVAEDETPFVIVDLSKLRQV 141
Query: 144 QIEIETNTAWVESGATLGELYYRIAEKSRTLAFPAGTCPIVGVGGHLSGGGYGMLMRKYG 203
+++++N+AW +GAT+GE+YYRI EKS+T FPAG C +G+GGHL GG YG +MRK+G
Sbjct: 142 DVDLDSNSAWAHAGATIGEVYYRIQEKSQTHGFPAGLCSSLGIGGHLVGGAYGSMMRKFG 201
Query: 204 LAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVLVPATVTLC 263
L ADNV+DA +VDANG+ DR +MGED+FWAIRGGGGGSFG+++AWK+KLV VPATVT+
Sbjct: 202 LGADNVLDARIVDANGQILDRAAMGEDVFWAIRGGGGGSFGVILAWKIKLVPVPATVTVF 261
Query: 264 FVSKTWEEGATNLIHRWQYVAHDLDENLFIGILLSGGNFTRQEGKTNPTALFFSLYLGKV 323
V+KT E+ T ++++W+ +A LD++LFI +++S + T + G + + + +LG
Sbjct: 262 TVTKTLEQDGTKVLYKWEQIADKLDDDLFIRVIISPASKTTKPGNRTISMSYQAQFLGDS 321
Query: 324 DELVSILNKTFPELGLAKKDCIETSWVESALIMGTGLQPGKSLEDLLSKASLTNGNTKIK 383
+ L+ ++ K+FPELGL KKDC E SW++S + + G + E LL+ SL + K K
Sbjct: 322 NRLLQVMQKSFPELGLTKKDCTEMSWIKSVMYI-AGFPNSAAPEALLAGKSLFKNHFKAK 381
Query: 384 SDYIKQPIPLAAVEGIWQRLKLEDIEMPQLLFAPYGGRMSQISESESPFSHRAGYLYKIG 443
SD++K+PIP+ +EG+W+R ED P ++ PYGG MS+ISESE PF HR G L+KI
Sbjct: 382 SDFVKEPIPVEGLEGLWERFLEED--SPLTIWNPYGGMMSRISESEIPFPHRNGTLFKIQ 441
Query: 444 YVTYWEQQDNDV-EKRHLSWIRELYSYMTPFVSKSPRAAYINYRDLDIGSNNKYGKTSYK 503
+++ W QD V E+RH+ WIRE+YSYM +VSK+PR AY+NYRDLD+G+N G+T +
Sbjct: 442 WLSTW--QDGKVSEERHMKWIREMYSYMEQYVSKNPRQAYVNYRDLDLGTNE--GETDAR 501
Query: 504 QASIWGLKYFGNNFNRLVDVKTRVDPHNFFRHEQSIPT 534
+ WG KY+ NF RLV +K DP NFFRHEQS+PT
Sbjct: 502 E---WGAKYYKGNFERLVKIKGEFDPDNFFRHEQSVPT 529
BLAST of Bhi08G000824 vs. TAIR10
Match:
AT5G44400.1 (FAD-binding Berberine family protein)
HSP 1 Score: 511.5 bits (1316), Expect = 6.2e-145
Identity = 260/516 (50.39%), Postives = 348/516 (67.44%), Query Frame = 0
Query: 26 SNSSEKHENFLECL---FHHSPNISKVIYTPFN--SSYSSILNFTLRNLRFSTPNTPKPL 85
S+ + + F+ C+ H + K + P S +S +L T +NLRF + PKP
Sbjct: 26 SSLASLQDQFINCVQRNTHVYFPLEKTFFAPTKNVSMFSQVLESTAQNLRFLKKSMPKPG 85
Query: 86 LIITPSHISHIQVAVICSKSYGLQIRTRSGGHDFEGLSYVAYL--PFIIVDLINLSSIQI 145
I +P H SH+Q ++ICSK + +R RSGGHD+EGLSYV+ + PFI++DL + + I
Sbjct: 86 FIFSPIHESHVQASIICSKKLRMHLRVRSGGHDYEGLSYVSQIDKPFILMDLSKMRQVNI 145
Query: 146 EIETNTAWVESGATLGELYYRIAEKSRTLAFPAGTCPIVGVGGHLSGGGYGMLMRKYGLA 205
I+ N+AWV+SGAT+GELYYRIAEKS+ FPAG C +G+GGH++GG YG +MRKYGL
Sbjct: 146 NIQDNSAWVQSGATVGELYYRIAEKSKVHGFPAGLCSSLGIGGHITGGAYGSMMRKYGLG 205
Query: 206 ADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVLVPATVTLCFV 265
ADNV+DA +VDANG+ DR +MGED FWAIRGG GGSFGI++AWK+KLV VP TVT+ V
Sbjct: 206 ADNVLDAKIVDANGKLLDRAAMGEDTFWAIRGGAGGSFGIILAWKIKLVPVPKTVTVFTV 265
Query: 266 SKTWEEGATN-LIHRWQYVAHDLDENLFIGILLSGGNFTRQEGKTNPTALFFSLYLGKVD 325
+KT ++ N +I +WQ VA L E LFI +L N G T + +L+LG
Sbjct: 266 TKTLQQDVGNKIISKWQRVADKLVEELFIRVLF---NVAGTGGNKTVTTSYNALFLGGKG 325
Query: 326 ELVSILNKTFPELGLAKKDCIETSWVES-ALIMGTGLQPGKSLEDLLSKASLTNGNTKIK 385
L++++ K+FPELGL KDCIE SW+ES A I G ++ L K+ + K K
Sbjct: 326 TLMNVMKKSFPELGLTFKDCIEMSWLESIAYISGFPTHTPTNVL-LQGKSPFPKVSFKAK 385
Query: 386 SDYIKQPIPLAAVEGIWQRLKLEDIEMPQLLFAPYGGRMSQISESESPFSHRAGYLYKIG 445
SD++K PIP + ++GI+++L EDI P +++ PYGG M++I ES+ PF HR G L+K+
Sbjct: 386 SDFVKTPIPESGLQGIFKKLLKEDI--PLMIWNPYGGMMAKIPESQIPFPHRKGVLFKVQ 445
Query: 446 YVTYWEQQDNDVEKRHLSWIRELYSYMTPFVSKSPRAAYINYRDLDIGSNNKYGKTSYKQ 505
YVT W D RH++WIR+LYSYMTP+VS +PR AY+NYRDLD+G N K KT KQ
Sbjct: 446 YVTSWLDSDKR-PSRHINWIRDLYSYMTPYVSSNPREAYVNYRDLDLGRNTKDVKTCIKQ 505
Query: 506 ASIWGLKYFGNNFNRLVDVKTRVDPHNFFRHEQSIP 533
A +WG YF NNFNRL+ +K +VDP NFFRHEQSIP
Sbjct: 506 AQVWGANYFKNNFNRLMMIKAKVDPENFFRHEQSIP 534
BLAST of Bhi08G000824 vs. TAIR10
Match:
AT5G44410.1 (FAD-binding Berberine family protein)
HSP 1 Score: 507.3 bits (1305), Expect = 1.2e-143
Identity = 246/507 (48.52%), Postives = 358/507 (70.61%), Query Frame = 0
Query: 32 HENFLECLFHH-SPNISKVIYTPFNSSYSSILNFTLRNLRFSTPNTPKPLLIITPSHISH 91
HENFL+CL H + + S++I+T + SY SILN +++N RF TPKP+ IITP +
Sbjct: 34 HENFLKCLSHRINEDDSRIIHTSKDPSYFSILNSSIQNPRFFVLETPKPVSIITPVQATD 93
Query: 92 IQVAVICSKSYGLQIRTRSGGHDFEGLSYVA-YLPFIIVDLINLSSIQIEIETNTAWVES 151
+Q + C++ +G+ IRTRSGGHD+EGLSY+A PF+++DL NL SI ++++ T WV+S
Sbjct: 94 VQSTIKCARLHGIHIRTRSGGHDYEGLSYMAKSRPFVVIDLRNLRSITLDVDNRTGWVQS 153
Query: 152 GATLGELYYRIAEKSRTLAFPAGTCPIVGVGGHLSGGGYGMLMRKYGLAADNVIDAYLVD 211
GAT+GELYY I + S++LAFPAG P VG+GG GGGYG LMRKYGL+ADNVIDA++VD
Sbjct: 154 GATIGELYYEIGKLSKSLAFPAGLYPTVGIGGQFGGGGYGTLMRKYGLSADNVIDAHIVD 213
Query: 212 ANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVLVPATVTLCFVSKTWEEGATNL 271
ANG F DR+ MGED FWAIRGGGG SF +V++WK++L+ VP+ VT+ V KT E+ A ++
Sbjct: 214 ANGSFLDRQGMGEDFFWAIRGGGGSSFSVVLSWKIRLLDVPSVVTVFKVVKTSEKEAVSI 273
Query: 272 IHRWQYVAHDLDENLFIGILLSGGNFTRQEGKTNPTALFFSLYLGKVDELVSILNKTFPE 331
I++WQY+A + +LFI +L + +T A F LYLG V +L++++ FPE
Sbjct: 274 INKWQYIADKVPNDLFIRAML--------QKETEVYASFPGLYLGPVSDLLALMKDKFPE 333
Query: 332 LGLAKKDCIETSWVESALIMGTGLQPGKSLEDLLSKASLTNGNTKIKSDYIKQPIPLAAV 391
LGL +C E SW+ES L G+S+E +L+K T+ + K K D+I++PIP A+
Sbjct: 334 LGLEIGNCREMSWIESVL----WFIKGESME-ILAKRKRTSRSFKGKDDFIEEPIPKTAI 393
Query: 392 EGIWQRLKLEDIEMPQLLFAPYGGRMSQISESESPFSHRAGYLYKIGYVTYWEQQDNDVE 451
+ +W+R + + + +++ P+GG+MS+I+++E PF HR G LY+I Y+ YW ++++ +
Sbjct: 394 QYLWRRFEAPEARLAKIILTPFGGKMSEIADNEIPFPHREGNLYEIQYLAYWSEEEDKNK 453
Query: 452 ---KRHLSWIRELYSYMTPFVSKSPRAAYINYRDLDIGSNNKYG-KTSYKQASIWGLKYF 511
+++L W+ +Y +MTP+VSKSPR AY+N+RD+D+G KT Y++A +WG+KYF
Sbjct: 454 TNTEKYLRWVESVYEFMTPYVSKSPRRAYVNFRDIDLGMYLGLNMKTKYEEAKVWGVKYF 513
Query: 512 GNNFNRLVDVKTRVDPHNFFRHEQSIP 533
NNF+RLV VKT VDP +FF EQSIP
Sbjct: 514 KNNFDRLVRVKTNVDPMDFFCDEQSIP 527
BLAST of Bhi08G000824 vs. TrEMBL
Match:
tr|A0A1S3BDA8|A0A1S3BDA8_CUCME (tetrahydrocannabinolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC103488637 PE=3 SV=1)
HSP 1 Score: 790.0 bits (2039), Expect = 3.3e-225
Identity = 373/512 (72.85%), Postives = 438/512 (85.55%), Query Frame = 0
Query: 25 ASNSSEKHENFLECLFHHSPN---ISKVIYTPFNSSYSSILNFTLRNLRFSTPNTPKPLL 84
AS S +K++NFL+CL HS ISKV+YTP NSSYSS+L+F++RNL FS P TPKPLL
Sbjct: 24 ASASPQKYQNFLQCLSEHSSKSYPISKVVYTPINSSYSSVLDFSIRNLLFSKPETPKPLL 83
Query: 85 IITPSHISHIQVAVICSKSYGLQIRTRSGGHDFEGLSYVAYLPFIIVDLINLSSIQIEIE 144
IITPSH+SHIQ AVICSKS+GLQIRTRSGGHDFEGLSYVAY PFI+VDLINL S+++E+E
Sbjct: 84 IITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGLSYVAYRPFIVVDLINLRSVKVEVE 143
Query: 145 TNTAWVESGATLGELYYRIAEKSRTLAFPAGTCPIVGVGGHLSGGGYGMLMRKYGLAADN 204
NTAWVESGATLGELYYRI EKSRTLAFPAG CP VG+GGH SGGGYG+++RK+GLAADN
Sbjct: 144 NNTAWVESGATLGELYYRIGEKSRTLAFPAGVCPTVGIGGHFSGGGYGLMLRKFGLAADN 203
Query: 205 VIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVLVPATVTLCFVSKT 264
VIDAYLVDANG+ D+ESMGEDLFWAIRGGGGGSFGIVVAWK+KLV VP TVT+C +
Sbjct: 204 VIDAYLVDANGKVLDKESMGEDLFWAIRGGGGGSFGIVVAWKIKLVPVPETVTICSTDRN 263
Query: 265 WEEGATNLIHRWQYVAHDLDENLFIGILLSGGNFTRQEGKTNPTALFFSLYLGKVDELVS 324
EE A LIHRWQYV + LDEN+++GI+L+GGN + Q G TNPTA FFSL+LG+ DELV+
Sbjct: 264 LEEDAIKLIHRWQYVGNKLDENIYLGIILTGGNASTQAGITNPTARFFSLFLGRADELVT 323
Query: 325 ILNKTFPELGLAKKDCIETSWVESALIMGTGLQPGKSLEDLLSKASLTNGNTKIKSDYIK 384
L+ FPELGL K+DC+E SWVES LI+ G+QP ++LE LL++ T +TKIKSDYI
Sbjct: 324 TLSTIFPELGLIKQDCVEASWVESTLIIPIGVQPIETLEPLLNRTLTTLDSTKIKSDYIT 383
Query: 385 QPIPLAAVEGIWQRLKLEDIEMPQLLFAPYGGRMSQISESESPFSHRAGYLYKIGYVTYW 444
+PIP+AA++ IWQRLK +DIE QL+F PYGGRMSQISESE+PFSHRA YL+KIGYV W
Sbjct: 384 EPIPVAAIKDIWQRLKTQDIETSQLVFVPYGGRMSQISESETPFSHRARYLFKIGYVVGW 443
Query: 445 EQQDNDVEKRHLSWIRELYSYMTPFVSKSPRAAYINYRDLDIGSNNKYGKTSYKQASIWG 504
+ Q +KRH+SW+RE+Y YM PFVSKSPRAAY NYRDLDIGSNNKYGKTSYK+AS+WG
Sbjct: 444 KDQSLKAKKRHISWLREVYEYMAPFVSKSPRAAYTNYRDLDIGSNNKYGKTSYKRASVWG 503
Query: 505 LKYFGNNFNRLVDVKTRVDPHNFFRHEQSIPT 534
LKYFGNNF+RLV VKT+VDP++FFRHEQSIPT
Sbjct: 504 LKYFGNNFDRLVYVKTKVDPYDFFRHEQSIPT 535
BLAST of Bhi08G000824 vs. TrEMBL
Match:
tr|A0A1S3BCV7|A0A1S3BCV7_CUCME (cannabidiolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC103488658 PE=3 SV=1)
HSP 1 Score: 783.1 bits (2021), Expect = 4.0e-223
Identity = 371/521 (71.21%), Postives = 439/521 (84.26%), Query Frame = 0
Query: 23 WVASNSSEKHENFLECLFHHSP---NISKVIYTPFNSSYSSILNFTLRNLRFSTPNTPKP 82
W +++S HE FL CL HHSP +I+K++YTP N+SYS +LNF++RNLRFSTPNTPKP
Sbjct: 23 WPSASSHVNHEEFLPCLLHHSPDSKSITKLVYTPINTSYSPVLNFSIRNLRFSTPNTPKP 82
Query: 83 LLIITPSHISHIQVAVICSKSYGLQIRTRSGGHDFEGLSYVAYLPFIIVDLINLSSIQIE 142
LLIITPS+ISH+Q AVICSKS+G+QIR RSGGHDFEGLSYVAY PF++VDLINLSS+ IE
Sbjct: 83 LLIITPSNISHVQAAVICSKSHGIQIRIRSGGHDFEGLSYVAYHPFVVVDLINLSSVTIE 142
Query: 143 IETNTAWVESGATLGELYYRIAEKSRTLAFPAGTCPIVGVGGHLSGGGYGMLMRKYGLAA 202
+E +TAWV+SGATLGELYYRIAEKSRTLAFPAG CP VGVGGH SGGG+G L+RKYGLAA
Sbjct: 143 VEQSTAWVQSGATLGELYYRIAEKSRTLAFPAGNCPTVGVGGHFSGGGFGTLLRKYGLAA 202
Query: 203 DNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVLVPATVTLCFVS 262
DNVIDAY+VDANGEFHDR+SMGEDLFWAIRGGGGGSFGIVVAWKVKLV VPATVT+C +S
Sbjct: 203 DNVIDAYIVDANGEFHDRKSMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTICTIS 262
Query: 263 KTWEEGATNLIHRWQYVAHDLDENLFIGILLSGGNFTRQEGKTNPTALFFSLYLGKVDEL 322
+T EE A LI +WQYVA+ LDE+LF+GI L GG Q K NP ALFFSL+LGK DEL
Sbjct: 263 RTLEEEAIKLIDQWQYVANKLDEDLFLGINLLGGKIVAQGDKINPIALFFSLFLGKADEL 322
Query: 323 VSILNKTFPELGLAKKDCIETSWVESALIMGTGLQ-PGKSLEDLLSKASLTNGNTKIKSD 382
++ILNKTFP+LGL K++C ETSW+ES + G GLQ + LE LL++ L NGN K+KSD
Sbjct: 323 MAILNKTFPQLGLTKEECKETSWIESVVYTGNGLQIEDQPLEVLLNRTPLANGNIKMKSD 382
Query: 383 YIKQPIPLAAVEGIWQRLKLEDIEMPQLLFAPYGGRMSQISESESPFSHRAGYLYKIGYV 442
Y+K+PI A ++ +WQRLK +DIE L+F PYGGRMSQISESE PFSHRAG LYKIGY+
Sbjct: 383 YVKEPISKATIKEVWQRLKSQDIEGANLVFIPYGGRMSQISESEIPFSHRAGNLYKIGYL 442
Query: 443 TYWEQQDNDVEKRHLSWIRELYSYMTPFVSKSPRAAYINYRDLDIGSNNKYGKTSYKQAS 502
T W + + EK HL+WIR++YSYMTPFV+KSPRAAY+NYRDLDIGSN+KYGKTSYK+A
Sbjct: 443 TGWFEPGLNAEKTHLNWIRDIYSYMTPFVTKSPRAAYVNYRDLDIGSNSKYGKTSYKRAH 502
Query: 503 IWGLKYFGNNFNRLVDVKTRVDPHNFFRHEQSIPTFSNKKV 540
+WG KYFG+NF+RLV VK VDP+NFFRHEQSIP +++
Sbjct: 503 VWGSKYFGSNFDRLVYVKNEVDPYNFFRHEQSIPVLLKRRI 543
BLAST of Bhi08G000824 vs. TrEMBL
Match:
tr|A0A1S3BDB7|A0A1S3BDB7_CUCME (tetrahydrocannabinolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC103488652 PE=3 SV=1)
HSP 1 Score: 782.3 bits (2019), Expect = 6.9e-223
Identity = 380/515 (73.79%), Postives = 430/515 (83.50%), Query Frame = 0
Query: 23 WVASNSSEKHENFLECLFHHSPN---ISKVIYTPFNSSYSSILNFTLRNLRFSTPNTPKP 82
WV++ E H+NFL CL HS ISKV+YTP NSSYSS+LNF++RN RFS P TPKP
Sbjct: 25 WVSAFPHE-HQNFLHCLPEHSSKSYPISKVVYTPINSSYSSVLNFSIRNFRFSKPETPKP 84
Query: 83 LLIITPSHISHIQVAVICSKSYGLQIRTRSGGHDFEGLSYVAYLPFIIVDLINLSSIQIE 142
LLIITPSH+SHIQ AVICSKS+GLQIRTRSGGHD+EGLSYVAY PFIIVDLIN+ SI ++
Sbjct: 85 LLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDYEGLSYVAYRPFIIVDLINIRSISVD 144
Query: 143 IETNTAWVESGATLGELYYRIAEKSRTLAFPAGTCPIVGVGGHLSGGGYGMLMRKYGLAA 202
I NTAWVESGATLGELYYRI EKSRTLAFPAG CP VGVGGH SGGGYG L+RKYGLAA
Sbjct: 145 IVNNTAWVESGATLGELYYRIGEKSRTLAFPAGICPTVGVGGHFSGGGYGFLLRKYGLAA 204
Query: 203 DNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVLVPATVTLCFVS 262
DNVIDAYLVDANG HDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLV VPA VT+C
Sbjct: 205 DNVIDAYLVDANGMAHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPALVTICSTI 264
Query: 263 KTWEEGATNLIHRWQYVAHDLDENLFIGILLSGGNFTRQEGKTNPTALFFSLYLGKVDEL 322
K E A +IH+WQYVA+ L E++F+GI+L+GGN + Q G PTA F+SL+LGKVDEL
Sbjct: 265 KNLEGDAVKVIHQWQYVANKLHEDIFLGIVLTGGNTSTQGGIKKPTATFYSLFLGKVDEL 324
Query: 323 VSILNKTFPELGLAKKDCIETSWVESALIMGTGL-QPGKSLEDLLSKASLTNGNTKIKSD 382
V+ L+ TFPE GL KKDC+E W+ES LI+ +G+ Q +SL+ LL++ T +TKIKSD
Sbjct: 325 VATLSTTFPEFGLIKKDCLEVRWIESTLIIASGVFQTVESLKPLLNRTPSTIDSTKIKSD 384
Query: 383 YIKQPIPLAAVEGIWQRLKLEDIEMPQLLFAPYGGRMSQISESESPFSHRAGYLYKIGYV 442
YIK+PIP AA+EGIWQRLK DIE PQL+F PYG RMSQISESE+PFSHRAGYLYKIGY+
Sbjct: 385 YIKKPIPKAAIEGIWQRLKAGDIEAPQLVFVPYGARMSQISESETPFSHRAGYLYKIGYM 444
Query: 443 TYWEQQDNDVEKRHLSWIRELYSYMTPFVSKSPRAAYINYRDLDIGSNNKYGKTSYKQAS 502
W+ Q+ + RH+SW RELY YMTPFVSKSPR AY+NYRDLDIGSNNKYGKTSYKQAS
Sbjct: 445 VGWKGQNLKAKNRHISWTRELYEYMTPFVSKSPRTAYVNYRDLDIGSNNKYGKTSYKQAS 504
Query: 503 IWGLKYFGNNFNRLVDVKTRVDPHNFFRHEQSIPT 534
IWGLKYFGNNF RLV VK +VDPH+FFRHEQSIPT
Sbjct: 505 IWGLKYFGNNFKRLVYVKNKVDPHDFFRHEQSIPT 538
BLAST of Bhi08G000824 vs. TrEMBL
Match:
tr|A0A1S3BDZ5|A0A1S3BDZ5_CUCME (tetrahydrocannabinolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC103488631 PE=3 SV=1)
HSP 1 Score: 773.5 bits (1996), Expect = 3.2e-220
Identity = 370/515 (71.84%), Postives = 431/515 (83.69%), Query Frame = 0
Query: 23 WVASNSSEKHENFLECLFHHSPN---ISKVIYTPFNSSYSSILNFTLRNLRFSTPNTPKP 82
WV S S KH+NFL+CL HS ISKV++TP NSSYSS+L+F++RNL FS P TPKP
Sbjct: 23 WV-SASPHKHQNFLQCLSEHSSKRYPISKVVHTPINSSYSSVLDFSIRNLLFSKPETPKP 82
Query: 83 LLIITPSHISHIQVAVICSKSYGLQIRTRSGGHDFEGLSYVAYLPFIIVDLINLSSIQIE 142
LLIITPSH+SHIQ AVICSKS+GLQIRTRSGGHDFEGLSYVAY PFIIVDLINL SI+I+
Sbjct: 83 LLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGLSYVAYHPFIIVDLINLRSIKID 142
Query: 143 IETNTAWVESGATLGELYYRIAEKSRTLAFPAGTCPIVGVGGHLSGGGYGMLMRKYGLAA 202
+ETNTAWVESGATLGELYYRI EKSRTLAFP G CP VGVGGH SGGGYG+++RK+GLAA
Sbjct: 143 VETNTAWVESGATLGELYYRIGEKSRTLAFPGGVCPTVGVGGHFSGGGYGLMLRKFGLAA 202
Query: 203 DNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVLVPATVTLCFVS 262
DNVIDAYLVDANG+ HDR+SMGEDLFWAIRGGGGGSFGIVVAWK+KLV VPATVT+C +
Sbjct: 203 DNVIDAYLVDANGKVHDRKSMGEDLFWAIRGGGGGSFGIVVAWKIKLVSVPATVTICSTN 262
Query: 263 KTWEEGATNLIHRWQYVAHDLDENLFIGILLSGGNF-TRQEGKTNPTALFFSLYLGKVDE 322
+ + L+H+WQYVA+ LDENLF+GI+L+GGN T QEG NP A FFSL+LGKVD
Sbjct: 263 RNLKGDGIKLVHQWQYVANKLDENLFLGIMLTGGNITTTQEGIANPIATFFSLFLGKVDI 322
Query: 323 LVSILNKTFPELGLAKKDCIETSWVESALIMGTGLQPGKSLEDLLSKASLTNGNTKIKSD 382
LV+ L+ FPELG K+DC+ETSW+ES LI TG+ +SLE LL++ N K+KSD
Sbjct: 323 LVATLSAIFPELGFTKEDCVETSWIESTLIAATGVGTVESLEPLLNRTPSNLENGKMKSD 382
Query: 383 YIKQPIPLAAVEGIWQRLKLEDIEMPQLLFAPYGGRMSQISESESPFSHRAGYLYKIGYV 442
Y K+PI +A +EG+WQRLK +DIE QL+F PYGGRMSQISE E+PFSHRAG LYKIGY
Sbjct: 383 YFKEPISIATIEGLWQRLKAKDIETSQLIFIPYGGRMSQISEMETPFSHRAGILYKIGYF 442
Query: 443 TYWEQQDNDVEKRHLSWIRELYSYMTPFVSKSPRAAYINYRDLDIGSNNKYGKTSYKQAS 502
W++Q +K+H+SWIR++Y YMTPFVSKSPR+AY NYRDLDIG+N KYGKTS +QAS
Sbjct: 443 VAWKEQSLKAKKKHISWIRDIYKYMTPFVSKSPRSAYANYRDLDIGANKKYGKTSVRQAS 502
Query: 503 IWGLKYFGNNFNRLVDVKTRVDPHNFFRHEQSIPT 534
IWGLKYFGNNF RLV VKT+VDP++FFRHEQSIPT
Sbjct: 503 IWGLKYFGNNFKRLVYVKTKVDPYDFFRHEQSIPT 536
BLAST of Bhi08G000824 vs. TrEMBL
Match:
tr|A0A1S3BDG0|A0A1S3BDG0_CUCME (cannabidiolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC103488675 PE=3 SV=1)
HSP 1 Score: 737.3 bits (1902), Expect = 2.5e-209
Identity = 346/514 (67.32%), Postives = 429/514 (83.46%), Query Frame = 0
Query: 23 WVASNSSEKHENFLECLFHHSPNISKVIYTPFNSSYSSILNFTLRNLRFSTPNTPKPLLI 82
WV ++S+ K + FL+CL +ISKVIY+P NSSY S+L+F+++NLRFS P TPKP+ I
Sbjct: 23 WVVASSNNKPQAFLQCL----SSISKVIYSPINSSYFSVLDFSIQNLRFSKPETPKPIAI 82
Query: 83 ITPSHISHIQVAVICSKSYG-LQIRTRSGGHDFEGLSYVAYLPFIIVDLINLSSIQIEIE 142
ITP H+S IQVA+ICS+++G LQIRTRSGGHDFEGLSYVA+ PFII+DLINL SI I+++
Sbjct: 83 ITPIHVSQIQVAIICSRTHGSLQIRTRSGGHDFEGLSYVAHHPFIILDLINLRSISIDVK 142
Query: 143 TNTAWVESGATLGELYYRIAEKSRTLAFPAGTCPIVGVGGHLSGGGYGMLMRKYGLAADN 202
NTAWV+SGAT+GELYY+IAEKSR+LAFPAG CP VG+GG +SGGGYG L+RKYGLA DN
Sbjct: 143 NNTAWVQSGATVGELYYKIAEKSRSLAFPAGVCPSVGIGGFVSGGGYGYLLRKYGLAVDN 202
Query: 203 VIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVLVPATVTLCFVSKT 262
V+DAYLVDANGE HDRESMGEDLFWAIRGGGGGSFGIVVAWK++LVLVP TVT+C +KT
Sbjct: 203 VLDAYLVDANGEVHDRESMGEDLFWAIRGGGGGSFGIVVAWKLRLVLVPETVTICISNKT 262
Query: 263 WEEGATNLIHRWQYVAHDLDENLFIGILLSGGNFTRQEGKTNPTALFFSLYLGKVDELVS 322
++GA +LI+ WQYVA LDENL++GILL+GGN + + GK NPTA F SL+LGK ++L+S
Sbjct: 263 VKDGAIDLIYEWQYVADKLDENLYLGILLNGGNISSEGGKPNPTASFISLFLGKANKLLS 322
Query: 323 ILNKTFPELGLAKKDCIETSWVESALIMGTGLQPGKSLEDLLSKASLTNGNTKIKSDYIK 382
ILNKTFP+LG+ KKDC +TSW+ESALI+ G SL LL++ S + G+ KIKSDY++
Sbjct: 323 ILNKTFPQLGVTKKDCTQTSWIESALIVINGSPTNNSLRTLLNRKSQSVGSFKIKSDYVQ 382
Query: 383 QPIPLAAVEGIWQRLKLEDIEMPQLLFAPYGGRMSQISESESPFSHRAGYLYKIGYVTYW 442
QP+PL A++GIW+RLK +D++ L PYGG+M +I + E+PF HRAG LY IGY+ W
Sbjct: 383 QPMPLDAIKGIWERLKSQDVKATTLDIVPYGGKMCKIFDFETPFPHRAGNLYMIGYLVGW 442
Query: 443 EQQDNDVEKRHLSWIRELYSYMTPFVSKSPRAAYINYRDLDIGSNNKYGKTSYKQASIWG 502
E Q ++E+RHLSWIRE+Y+YMTPFVSK PRAAY+NYRDLDIG+N +YGKTS+ QASIWG
Sbjct: 443 ENQSKEIEERHLSWIREIYNYMTPFVSKFPRAAYVNYRDLDIGTNTEYGKTSHDQASIWG 502
Query: 503 LKYFGNNFNRLVDVKTRVDPHNFFRHEQSIPTFS 536
KYF NFNRLV VKT+VDP++FFRHEQSIP S
Sbjct: 503 FKYFDKNFNRLVHVKTKVDPYDFFRHEQSIPILS 532
BLAST of Bhi08G000824 vs. NCBI nr
Match:
XP_022994835.1 (berberine bridge enzyme-like 18 [Cucurbita maxima])
HSP 1 Score: 849.0 bits (2192), Expect = 9.0e-243
Identity = 411/541 (75.97%), Postives = 467/541 (86.32%), Query Frame = 0
Query: 1 MKSSPLILXXXXXXXXXXXXAPWVASNSSEKHENFLECLFHHS---PNISKVIYTPFNSS 60
M SSPL L + WV+SN + H+NFL+CL H+S +IS V+YTPFNSS
Sbjct: 6 MNSSPL-LPLSLTLLVLCSCSSWVSSNFVKPHQNFLQCLSHYSQDGSSISDVLYTPFNSS 65
Query: 61 YSSILNFTLRNLRFSTPNTPKPLLIITPSHISHIQVAVICSKSYGLQIRTRSGGHDFEGL 120
YSS+LN TLRNLRFSTP+TPKPLLIITP H+SHIQ AV+CSKS+GLQIRTRSGGHDFEGL
Sbjct: 66 YSSVLNITLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHDFEGL 125
Query: 121 SYVAYLPFIIVDLINLSSIQIEIETNTAWVESGATLGELYYRIAEKSRTLAFPAGTCPIV 180
SYVA LPFIIVDLINL+SI I++E+ TAWV+SGATLGELYYRIA+KSRTLAFPAG CP V
Sbjct: 126 SYVASLPFIIVDLINLNSISIDVESKTAWVQSGATLGELYYRIAKKSRTLAFPAGICPTV 185
Query: 181 GVGGHLSGGGYGMLMRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG 240
GVGGH SGGGYGML+RKYGLAADNVIDA LVDANG+F +R+ MGEDLFWAIRGGGGGSFG
Sbjct: 186 GVGGHFSGGGYGMLLRKYGLAADNVIDAQLVDANGKFVNRKLMGEDLFWAIRGGGGGSFG 245
Query: 241 IVVAWKVKLVLVPATVTLCFVSKTWEEGATNLIHRWQYVAHDLDENLFIGILLSGGNFTR 300
IVVAWKVKLV VPA+VTLC +KT E+GAT LIH+WQYVAH L+ N F+GILL+GGNFTR
Sbjct: 246 IVVAWKVKLVPVPASVTLCMANKTSEDGATKLIHQWQYVAHKLENNAFLGILLAGGNFTR 305
Query: 301 QEGKTNPTALFFSLYLGKVDELVSILNKTFPELGLAKKDCIETSWVESALIMGTGLQPGK 360
Q GK NP ALFFSLYLGK DEL++ILN TFPELGL+KKDC+ETSWVE+ALIMGTGLQ G
Sbjct: 306 QGGKINPLALFFSLYLGKADELLTILNTTFPELGLSKKDCMETSWVETALIMGTGLQLGS 365
Query: 361 SLEDLLSKASLTNGNTKIKSDYIKQPIPLAAVEGIWQRLKLEDIEMPQLLFAPYGGRMSQ 420
LEDLLSKA L NG+TKIKSDYIK+PIP+AA+EGIW+RL+ EDIEMPQ +F PYGG+M Q
Sbjct: 366 KLEDLLSKAPLVNGSTKIKSDYIKEPIPMAAIEGIWERLRYEDIEMPQHIFVPYGGKMGQ 425
Query: 421 ISESESPFSHRAGYLYKIGYVTYWEQQDNDVEKRHLSWIRELYSYMTPFVSKSPRAAYIN 480
I E E+PFSHR YLYKIGY+ W +Q + EKRH+ WIRELYSYMTPFVSKSPRAAY+N
Sbjct: 426 IPEFETPFSHRGEYLYKIGYIVNWAEQGIEAEKRHIDWIRELYSYMTPFVSKSPRAAYLN 485
Query: 481 YRDLDIGSNNKYGKTSYKQASIWGLKYFG-NNFNRLVDVKTRVDPHNFFRHEQSIPTFSN 538
YRDLDIGSNNKYG+TSY+QASIWG KYFG NF+RLV VKT+VDP++FFRHEQSIPT S+
Sbjct: 486 YRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIPTLSS 545
BLAST of Bhi08G000824 vs. NCBI nr
Match:
XP_022954543.1 (berberine bridge enzyme-like 18 [Cucurbita moschata])
HSP 1 Score: 840.5 bits (2170), Expect = 3.2e-240
Identity = 407/541 (75.23%), Postives = 465/541 (85.95%), Query Frame = 0
Query: 1 MKSSPLILXXXXXXXXXXXXAPWVASNSSEKHENFLECLFHHS---PNISKVIYTPFNSS 60
M SSPL L + WV+SN + H+NFL+CL H+S +IS V+YTPFNSS
Sbjct: 6 MNSSPL-LPLSLTLLVLCSCSSWVSSNFVKPHQNFLQCLSHYSQDGSSISDVLYTPFNSS 65
Query: 61 YSSILNFTLRNLRFSTPNTPKPLLIITPSHISHIQVAVICSKSYGLQIRTRSGGHDFEGL 120
YSS+LN TLRNLRFSTP+TPKPLLIITP H+SHIQ AV+CSKS+GLQIRTRSGGHDFEGL
Sbjct: 66 YSSVLNITLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHDFEGL 125
Query: 121 SYVAYLPFIIVDLINLSSIQIEIETNTAWVESGATLGELYYRIAEKSRTLAFPAGTCPIV 180
SYVA LPFIIVDLINL+SI I++E TAWV+SGATLGELYY IA+KS+TLAFPAG CP V
Sbjct: 126 SYVASLPFIIVDLINLNSISIDVENKTAWVQSGATLGELYYSIAKKSQTLAFPAGICPTV 185
Query: 181 GVGGHLSGGGYGMLMRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG 240
GVGGH SGGGYGML+RKYGLAADNVIDA LVDANG+F +R+ MGEDLFWAIRGGGGGSFG
Sbjct: 186 GVGGHFSGGGYGMLLRKYGLAADNVIDAQLVDANGKFVNRQLMGEDLFWAIRGGGGGSFG 245
Query: 241 IVVAWKVKLVLVPATVTLCFVSKTWEEGATNLIHRWQYVAHDLDENLFIGILLSGGNFTR 300
IVVAWKVKLV VPA+VTLC +KT E+GAT LIH+WQYVAH L+ N F+GILL+GGNFTR
Sbjct: 246 IVVAWKVKLVPVPASVTLCMANKTSEDGATKLIHQWQYVAHKLENNAFLGILLAGGNFTR 305
Query: 301 QEGKTNPTALFFSLYLGKVDELVSILNKTFPELGLAKKDCIETSWVESALIMGTGLQPGK 360
Q GK NP ALFFSLYLGK +EL++ILN TFPELGL+KKDC+ETSWVE+ALIMGTGLQ G
Sbjct: 306 QGGKINPLALFFSLYLGKANELLTILNTTFPELGLSKKDCMETSWVETALIMGTGLQLGS 365
Query: 361 SLEDLLSKASLTNGNTKIKSDYIKQPIPLAAVEGIWQRLKLEDIEMPQLLFAPYGGRMSQ 420
LEDLLSKA+L NG+TKIKSDYIK+PIP+AA+EGIW+RL+ EDIEMPQ +F PYGG+M Q
Sbjct: 366 KLEDLLSKATLVNGSTKIKSDYIKEPIPMAAIEGIWERLRYEDIEMPQHIFVPYGGKMGQ 425
Query: 421 ISESESPFSHRAGYLYKIGYVTYWEQQDNDVEKRHLSWIRELYSYMTPFVSKSPRAAYIN 480
I E E+PFSHR LYKIGY+ W +Q + EKRH+ WIRELYSYMTPFVSKSPRAAY+N
Sbjct: 426 IPEFETPFSHRGENLYKIGYIVNWAEQGIEAEKRHIDWIRELYSYMTPFVSKSPRAAYLN 485
Query: 481 YRDLDIGSNNKYGKTSYKQASIWGLKYFG-NNFNRLVDVKTRVDPHNFFRHEQSIPTFSN 538
YRDLDIGSNNKYG+TSY+QASIWG KYFG NF+RLV VKT+VDP++FFRHEQSIPT S+
Sbjct: 486 YRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIPTLSS 545
BLAST of Bhi08G000824 vs. NCBI nr
Match:
XP_023542525.1 (berberine bridge enzyme-like 18 [Cucurbita pepo subsp. pepo])
HSP 1 Score: 838.6 bits (2165), Expect = 1.2e-239
Identity = 407/542 (75.09%), Postives = 467/542 (86.16%), Query Frame = 0
Query: 1 MKSSPLILXXXXXXXXXXXXAPWVASNSSEKHENFLECLFHHS---PNISKVIYTPFNSS 60
M SSPL L + WV++N + H++FL+CL H+S +IS V+YTPFNSS
Sbjct: 6 MNSSPL-LPLSLTLLVLCSCSSWVSANFVKPHQSFLQCLSHYSQDASSISDVLYTPFNSS 65
Query: 61 YSSILNFTLRNLRFSTPNTPKPLLIITPSHISHIQVAVICSKSYGLQIRTRSGGHDFEGL 120
YSS+LN TLRNLRFSTP+TPKPLLIITP H+SHIQ AV+CSKS+GLQIRTRSGGHDFEGL
Sbjct: 66 YSSVLNITLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHDFEGL 125
Query: 121 SYVAYLPFIIVDLINLSSIQIEIETNTAWVESGATLGELYYRIAEKSRTLAFPAGTCPIV 180
SYVA LPFIIVDLINL+SI I++E+ TAWV+SGATLGELYY IA+KS+TLAFPAG CP V
Sbjct: 126 SYVASLPFIIVDLINLNSISIDVESKTAWVQSGATLGELYYSIAKKSQTLAFPAGICPTV 185
Query: 181 GVGGHLSGGGYGMLMRKYGLAADNVIDAYLVDANGEFHDRES-MGEDLFWAIRGGGGGSF 240
GVGGH SGGGYGML+RKYGLAADNVIDA LVDANG+F +R+ MGEDLFWAIRGGGGGSF
Sbjct: 186 GVGGHFSGGGYGMLLRKYGLAADNVIDAQLVDANGKFVNRKKLMGEDLFWAIRGGGGGSF 245
Query: 241 GIVVAWKVKLVLVPATVTLCFVSKTWEEGATNLIHRWQYVAHDLDENLFIGILLSGGNFT 300
GIVVAWKVKLV VPA+VTLC +KT E+GAT LIH+WQYVAH L+ N F+GILL+GGNFT
Sbjct: 246 GIVVAWKVKLVPVPASVTLCMANKTSEDGATKLIHQWQYVAHKLENNAFLGILLAGGNFT 305
Query: 301 RQEGKTNPTALFFSLYLGKVDELVSILNKTFPELGLAKKDCIETSWVESALIMGTGLQPG 360
RQ GK NP ALFFSLYLGK DEL++ILN TFPELGL+KKDC+ETSWVE+ALIMGTGLQ G
Sbjct: 306 RQGGKINPLALFFSLYLGKADELLTILNTTFPELGLSKKDCMETSWVETALIMGTGLQFG 365
Query: 361 KSLEDLLSKASLTNGNTKIKSDYIKQPIPLAAVEGIWQRLKLEDIEMPQLLFAPYGGRMS 420
LEDLLSKA+L NG+TKIKSDYIK+PIP+AA+EGIW+RL+ EDIEMPQ +F PYGG+M
Sbjct: 366 SKLEDLLSKATLVNGSTKIKSDYIKEPIPMAAIEGIWERLRYEDIEMPQHIFVPYGGKMG 425
Query: 421 QISESESPFSHRAGYLYKIGYVTYWEQQDNDVEKRHLSWIRELYSYMTPFVSKSPRAAYI 480
QI E E+PFSHR YLYKIGY+ W +Q + EKRH+ WIRELYSYMTPFVSKSPRAAY+
Sbjct: 426 QIPEFETPFSHRGEYLYKIGYIVNWAEQGIEAEKRHIDWIRELYSYMTPFVSKSPRAAYL 485
Query: 481 NYRDLDIGSNNKYGKTSYKQASIWGLKYFG-NNFNRLVDVKTRVDPHNFFRHEQSIPTFS 538
NYRDLDIGSNNKYG+TSY+QASIWG KYFG NF+RLV VKT+VDP++FFRHEQSIPT S
Sbjct: 486 NYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIPTLS 545
BLAST of Bhi08G000824 vs. NCBI nr
Match:
XP_023542868.1 (berberine bridge enzyme-like 18 [Cucurbita pepo subsp. pepo])
HSP 1 Score: 837.8 bits (2163), Expect = 2.1e-239
Identity = 403/541 (74.49%), Postives = 461/541 (85.21%), Query Frame = 0
Query: 1 MKSSPLILXXXXXXXXXXXXAPWVASNSSEKHENFLECLFHHS---PNISKVIYTPFNSS 60
M SSPL L + WV++N + H+NFL+CL H+S +ISK++YTPFNSS
Sbjct: 6 MNSSPL-LPLSLTLLVLCSCSSWVSANFGKPHQNFLQCLSHYSQDASSISKILYTPFNSS 65
Query: 61 YSSILNFTLRNLRFSTPNTPKPLLIITPSHISHIQVAVICSKSYGLQIRTRSGGHDFEGL 120
YS++LNFTLRNLRFSTP+TPKPLLIITP H+SHIQ A++CSKSYGLQIRTRSGGHD EGL
Sbjct: 66 YSNVLNFTLRNLRFSTPHTPKPLLIITPLHVSHIQAAILCSKSYGLQIRTRSGGHDIEGL 125
Query: 121 SYVAYLPFIIVDLINLSSIQIEIETNTAWVESGATLGELYYRIAEKSRTLAFPAGTCPIV 180
SYVA LPFIIVDLINL+SI I++E+ TAWV+SGATLGELYYRIA+KSRTLAFPAG CP V
Sbjct: 126 SYVASLPFIIVDLINLNSISIDVESKTAWVQSGATLGELYYRIADKSRTLAFPAGICPTV 185
Query: 181 GVGGHLSGGGYGMLMRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG 240
GVGGH SGGGYG+L+RKYGLAADNVIDA LVDANG+F DR+ MGEDLFWAIRGGGGGSFG
Sbjct: 186 GVGGHFSGGGYGLLLRKYGLAADNVIDAQLVDANGKFFDRKLMGEDLFWAIRGGGGGSFG 245
Query: 241 IVVAWKVKLVLVPATVTLCFVSKTWEEGATNLIHRWQYVAHDLDENLFIGILLSGGNFTR 300
IVVAWKVKLV VPA VTLC V++T E+GAT LIH+WQYVAH L+ NLF+ ILL GGNFTR
Sbjct: 246 IVVAWKVKLVSVPAKVTLCVVNRTLEDGATKLIHQWQYVAHKLENNLFLSILLEGGNFTR 305
Query: 301 QEGKTNPTALFFSLYLGKVDELVSILNKTFPELGLAKKDCIETSWVESALIMGTGLQPGK 360
Q K NP ALFFSLYLGK DEL++ILN TFPELGL+KKDC+ETSWVE+ALIMGTGLQPG
Sbjct: 306 QGSKINPLALFFSLYLGKADELLTILNTTFPELGLSKKDCMETSWVETALIMGTGLQPGS 365
Query: 361 SLEDLLSKASLTNGNTKIKSDYIKQPIPLAAVEGIWQRLKLEDIEMPQLLFAPYGGRMSQ 420
LEDLLSK L TK+KSDY+K+PIP+AA+EGIWQRL+ DIE PQ++F PYGG+MSQ
Sbjct: 366 KLEDLLSKTPLMTVRTKMKSDYVKEPIPMAAIEGIWQRLRSGDIETPQIIFIPYGGKMSQ 425
Query: 421 ISESESPFSHRAGYLYKIGYVTYWEQQDNDVEKRHLSWIRELYSYMTPFVSKSPRAAYIN 480
ISE E+PFSHR Y YKIGY T W + D E +HL+WIRELYSYM PFVSKSPRAAY+N
Sbjct: 426 ISELETPFSHRGEYSYKIGYTTNWVEGGIDAENKHLNWIRELYSYMAPFVSKSPRAAYLN 485
Query: 481 YRDLDIGSNNKYGKTSYKQASIWGLKYFG-NNFNRLVDVKTRVDPHNFFRHEQSIPTFSN 538
YRDLDIGSNNKYG+TSY+QASIWG KYFG NF+RLV VKT+VDP++FFRHEQSIPT S+
Sbjct: 486 YRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIPTLSS 545
BLAST of Bhi08G000824 vs. NCBI nr
Match:
XP_022954548.1 (berberine bridge enzyme-like 18 [Cucurbita moschata])
HSP 1 Score: 835.1 bits (2156), Expect = 1.4e-238
Identity = 405/541 (74.86%), Postives = 461/541 (85.21%), Query Frame = 0
Query: 1 MKSSPLILXXXXXXXXXXXXAPWVASNSSEKHENFLECLFHHS---PNISKVIYTPFNSS 60
M SSPL L + WV+SN ++ HENFL+CL H+S +IS V+YTPFNSS
Sbjct: 6 MNSSPL-LPLSLTLLVLCSCSSWVSSNFAKPHENFLQCLSHYSQDASSISNVLYTPFNSS 65
Query: 61 YSSILNFTLRNLRFSTPNTPKPLLIITPSHISHIQVAVICSKSYGLQIRTRSGGHDFEGL 120
YS +LNFTLRNLRFSTP+TPKPLLIITP H+SHIQ AV+CSKS+GLQIRTRSGGHD EGL
Sbjct: 66 YSDVLNFTLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHDIEGL 125
Query: 121 SYVAYLPFIIVDLINLSSIQIEIETNTAWVESGATLGELYYRIAEKSRTLAFPAGTCPIV 180
SYVA LPFIIVDLINL+SI I++E+ TAWV+SGATLGELYYRIAEKSR+LAFPAG CP V
Sbjct: 126 SYVASLPFIIVDLINLNSISIDVESKTAWVQSGATLGELYYRIAEKSRSLAFPAGICPTV 185
Query: 181 GVGGHLSGGGYGMLMRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG 240
GVGGHLSGGGYG+L+RKYGLAADNVIDA LVDANG+F DR+ MGEDLFWAIRGGGGGSFG
Sbjct: 186 GVGGHLSGGGYGLLLRKYGLAADNVIDAQLVDANGKFFDRKLMGEDLFWAIRGGGGGSFG 245
Query: 241 IVVAWKVKLVLVPATVTLCFVSKTWEEGATNLIHRWQYVAHDLDENLFIGILLSGGNFTR 300
IVVAWKV LV VPATVTLC V+KT E+GAT LIH+WQYVAH L+ NLF+GILL GGNFTR
Sbjct: 246 IVVAWKVNLVSVPATVTLCVVNKTVEDGATKLIHQWQYVAHKLENNLFLGILLEGGNFTR 305
Query: 301 QEGKTNPTALFFSLYLGKVDELVSILNKTFPELGLAKKDCIETSWVESALIMGTGLQPGK 360
Q K NP ALFFS YLGK DEL++ILN TFPEL L+KKDCIETSWVE+ALIMGTGLQPG
Sbjct: 306 QGSKINPLALFFSFYLGKADELLTILNTTFPELDLSKKDCIETSWVETALIMGTGLQPGS 365
Query: 361 SLEDLLSKASLTNGNTKIKSDYIKQPIPLAAVEGIWQRLKLEDIEMPQLLFAPYGGRMSQ 420
LEDLLSK L +TK+KSDY+K+PIP+AA+EGI QRL+ DIE PQ++F PYGG+MS+
Sbjct: 366 KLEDLLSKTPLMTVSTKVKSDYVKEPIPMAAIEGIRQRLRSGDIETPQIIFIPYGGKMSE 425
Query: 421 ISESESPFSHRAGYLYKIGYVTYWEQQDNDVEKRHLSWIRELYSYMTPFVSKSPRAAYIN 480
ISE E+PFSHR Y YKIGY T W ++ D E +HL+WIRELYSYM PFVSKSPRAAY+N
Sbjct: 426 ISELETPFSHRGEYSYKIGYTTSWVERGIDAENKHLNWIRELYSYMAPFVSKSPRAAYLN 485
Query: 481 YRDLDIGSNNKYGKTSYKQASIWGLKYFG-NNFNRLVDVKTRVDPHNFFRHEQSIPTFSN 538
YRDLDIGSNNKYG+TSY+QA IWG KYFG NF+RLV VKT+VDP++FFRHEQSIPTFS+
Sbjct: 486 YRDLDIGSNNKYGRTSYEQAGIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIPTFSS 545
The following BLAST results are available for this feature:
