CSPI01G24740 (gene) Wild cucumber (PI 183967)

BLAST of CSPI01G24740 vs. Swiss-Prot
Match: CBDAS_CANSA (Cannabidiolic acid synthase OS=Cannabis sativa GN=CBDAS PE=1 SV=1)

HSP 1 Score: 551.6 bits (1420), Expect = 9.8e-156
Identity = 269/530 (50.75%), Postives = 372/530 (70.19%), Query Frame = 1

Query: 22  IFIVLTF-VWAASAHNHESFLHCLSHHSPNTSSISKIIYTPTNPSYSSVLNFSIHNLRFT 81
           IF   +F +  + A+  E+FL C S + PN ++  K++YT  NP Y SVLN +IHNLRFT
Sbjct: 15  IFFFFSFNIQTSIANPRENFLKCFSQYIPNNATNLKLVYTQNNPLYMSVLNSTIHNLRFT 74

Query: 82  SPKTPKPQVIVTPFHVSQIQASIICAKNTGFQIRTRSGGHDYEGLSYVSDVSFVVVDLIN 141
           S  TPKP VIVTP HVS IQ +I+C+K  G QIRTRSGGHD EG+SY+S V FV+VDL N
Sbjct: 75  SDTTPKPLVIVTPSHVSHIQGTILCSKKVGLQIRTRSGGHDSEGMSYISQVPFVIVDLRN 134

Query: 142 LRSISVDAENNVAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHFSGGGYGLML 201
           +RSI +D  +  AWV++GAT+GE+YY + EK++ L   AG CPTV  GGHF GGGYG ++
Sbjct: 135 MRSIKIDVHSQTAWVEAGATLGEVYYWVNEKNENLSLAAGYCPTVCAGGHFGGGGYGPLM 194

Query: 202 RKFGLAADNVIDAYFIDVNGKLHDRKSMGEDVFWAIRGSGGASYGIVLAWKIKLLPVPPI 261
           R +GLAADN+IDA+ ++V+GK+ DRKSMGED+FWA+RG G  S+GI++AWKI+L+ VP  
Sbjct: 195 RNYGLAADNIIDAHLVNVHGKVLDRKSMGEDLFWALRGGGAESFGIIVAWKIRLVAVPK- 254

Query: 262 VTVFTIARTLE-QNATDIIHRWQYVSSKQDDKLFIRIILTGINTTNSQHGNKRTIEAAFN 321
            T+F++ + +E      ++++WQ ++ K D  L +       N T++Q  NK  I   F+
Sbjct: 255 STMFSVKKIMEIHELVKLVNKWQNIAYKYDKDLLLMTHFITRNITDNQGKNKTAIHTYFS 314

Query: 322 SLFLGKIEELVPIMQKTFPELGLTKEDCIEMSWIESVLYFAGFSRGQPLN----VLLDRR 381
           S+FLG ++ LV +M K+FPELG+ K DC ++SWI+++++++G       N    +LLD R
Sbjct: 315 SVFLGGVDSLVDLMNKSFPELGIKKTDCRQLSWIDTIIFYSGVVNYDTDNFNKEILLD-R 374

Query: 382 PLTPKRFFKAKSDYVNEPIPKAGLEGIWEFFNEEEAEAAVLILSPYGGIMDKISESEIPF 441
                  FK K DYV +PIP++    I E   EE+  A +  L PYGGIMD+ISES IPF
Sbjct: 375 SAGQNGAFKIKLDYVKKPIPESVFVQILEKLYEEDIGAGMYALYPYGGIMDEISESAIPF 434

Query: 442 PHRAGNLYKIQHLVYWDEEGEDIAKRHINWIRKLYSYMAPFVSKNPRAAYINYRDLDIGT 501
           PHRAG LY++ ++  W+++ ++  ++H+NWIR +Y++M P+VSKNPR AY+NYRDLDIG 
Sbjct: 435 PHRAGILYELWYICSWEKQEDN--EKHLNWIRNIYNFMTPYVSKNPRLAYLNYRDLDIGI 494

Query: 502 NEKNGRNTSYNEASVWGVKYFKGNFKRLVSVKTKVDPSNFFKNEQSIPSL 546
           N+    N +Y +A +WG KYF  NF RLV VKT VDP+NFF+NEQSIP L
Sbjct: 495 NDPKNPN-NYTQARIWGEKYFGKNFDRLVKVKTLVDPNNFFRNEQSIPPL 539

BLAST of CSPI01G24740 vs. Swiss-Prot
Match: THCAS_CANSA (Tetrahydrocannabinolic acid synthase OS=Cannabis sativa PE=1 SV=1)

HSP 1 Score: 549.3 bits (1414), Expect = 4.9e-155
Identity = 269/533 (50.47%), Postives = 369/533 (69.23%), Query Frame = 1

Query: 22  IFIVLTF-VWAASAHNHESFLHCLSHHSPNTSSISKIIYTPTNPSYSSVLNFSIHNLRFT 81
           IF  L+F +  + A+  E+FL C S H PN  +  K++YT  +  Y S+LN +I NLRF 
Sbjct: 15  IFFFLSFHIQISIANPRENFLKCFSKHIPNNVANPKLVYTQHDQLYMSILNSTIQNLRFI 74

Query: 82  SPKTPKPQVIVTPFHVSQIQASIICAKNTGFQIRTRSGGHDYEGLSYVSDVSFVVVDLIN 141
           S  TPKP VIVTP + S IQA+I+C+K  G QIRTRSGGHD EG+SY+S V FVVVDL N
Sbjct: 75  SDTTPKPLVIVTPSNNSHIQATILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVVVDLRN 134

Query: 142 LRSISVDAENNVAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHFSGGGYGLML 201
           + SI +D  +  AWV++GAT+GE+YY I EK++ L FP G CPTVGVGGHFSGGGYG ++
Sbjct: 135 MHSIKIDVHSQTAWVEAGATLGEVYYWINEKNENLSFPGGYCPTVGVGGHFSGGGYGALM 194

Query: 202 RKFGLAADNVIDAYFIDVNGKLHDRKSMGEDVFWAIRGSGGASYGIVLAWKIKLLPVPPI 261
           R +GLAADN+IDA+ ++V+GK+ DRKSMGED+FWAIRG GG ++GI+ AWKIKL+ VP  
Sbjct: 195 RNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIKLVAVPSK 254

Query: 262 VTVFTIARTLE-QNATDIIHRWQYVSSKQDDKLFIRIILTGINTTNSQHGNKRTIEAAFN 321
            T+F++ + +E      + ++WQ ++ K D  L +       N T++   NK T+   F+
Sbjct: 255 STIFSVKKNMEIHGLVKLFNKWQNIAYKYDKDLVLMTHFITKNITDNHGKNKTTVHGYFS 314

Query: 322 SLFLGKIEELVPIMQKTFPELGLTKEDCIEMSWIESVLYFAGFSRGQPLN----VLLDRR 381
           S+F G ++ LV +M K+FPELG+ K DC E SWI++ ++++G       N    +LLD R
Sbjct: 315 SIFHGGVDSLVDLMNKSFPELGIKKTDCKEFSWIDTTIFYSGVVNFNTANFKKEILLD-R 374

Query: 382 PLTPKRFFKAKSDYVNEPIPKAGLEGIWEFFNEEEAEAAVLILSPYGGIMDKISESEIPF 441
               K  F  K DYV +PIP+  +  I E   EE+  A + +L PYGGIM++ISES IPF
Sbjct: 375 SAGKKTAFSIKLDYVKKPIPETAMVKILEKLYEEDVGAGMYVLYPYGGIMEEISESAIPF 434

Query: 442 PHRAGNLYKIQHLVYWDEEGEDIAKRHINWIRKLYSYMAPFVSKNPRAAYINYRDLDIG- 501
           PHRAG +Y++ +   W+++ ++  ++HINW+R +Y++  P+VS+NPR AY+NYRDLD+G 
Sbjct: 435 PHRAGIMYELWYTASWEKQEDN--EKHINWVRSVYNFTTPYVSQNPRLAYLNYRDLDLGK 494

Query: 502 TNEKNGRNTSYNEASVWGVKYFKGNFKRLVSVKTKVDPSNFFKNEQSIPSLKP 548
           TN  +  N  Y +A +WG KYF  NF RLV VKTKVDP+NFF+NEQSIP L P
Sbjct: 495 TNHASPNN--YTQARIWGEKYFGKNFNRLVKVKTKVDPNNFFRNEQSIPPLPP 542

BLAST of CSPI01G24740 vs. Swiss-Prot
Match: THCAI_CANSA (Inactive tetrahydrocannabinolic acid synthase OS=Cannabis sativa PE=3 SV=1)

HSP 1 Score: 547.7 bits (1410), Expect = 1.4e-154
Identity = 267/532 (50.19%), Postives = 364/532 (68.42%), Query Frame = 1

Query: 22  IFIVLTF-VWAASAHNHESFLHCLSHHSPNTSSISKIIYTPTNPSYSSVLNFSIHNLRFT 81
           IF  L+F +  + A+  E+FL C S + PN  +  K IYT  +  Y SVLN +I NLRFT
Sbjct: 15  IFFFLSFNIQISIANPQENFLKCFSEYIPNNPANPKFIYTQHDQLYMSVLNSTIQNLRFT 74

Query: 82  SPKTPKPQVIVTPFHVSQIQASIICAKNTGFQIRTRSGGHDYEGLSYVSDVSFVVVDLIN 141
           S  TPKP VIVTP +VS IQASI+C+K  G QIRTRSGGHD EGLSY+S V F +VDL N
Sbjct: 75  SDTTPKPLVIVTPSNVSHIQASILCSKKVGLQIRTRSGGHDAEGLSYISQVPFAIVDLRN 134

Query: 142 LRSISVDAENNVAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHFSGGGYGLML 201
           + ++ VD  +  AWV++GAT+GE+YY I E ++   FP G CPTVGVGGHFSGGGYG ++
Sbjct: 135 MHTVKVDIHSQTAWVEAGATLGEVYYWINEMNENFSFPGGYCPTVGVGGHFSGGGYGALM 194

Query: 202 RKFGLAADNVIDAYFIDVNGKLHDRKSMGEDVFWAIRGSGGASYGIVLAWKIKLLPVPPI 261
           R +GLAADN+IDA+ ++V+GK+ DRKSMGED+FWAIRG GG ++GI+ AWKIKL+ VP  
Sbjct: 195 RNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIKLVVVPSK 254

Query: 262 VTVFTIARTLE-QNATDIIHRWQYVSSKQDDKLFIRIILTGINTTNSQHGNKRTIEAAFN 321
            T+F++ + +E      + ++WQ ++ K D  L +       N T++   NK T+   F+
Sbjct: 255 ATIFSVKKNMEIHGLVKLFNKWQNIAYKYDKDLMLTTHFRTRNITDNHGKNKTTVHGYFS 314

Query: 322 SLFLGKIEELVPIMQKTFPELGLTKEDCIEMSWIESVLYFAGFSRGQPLN----VLLDRR 381
           S+FLG ++ LV +M K+FPELG+ K DC E+SWI++ ++++G       N    +LLD R
Sbjct: 315 SIFLGGVDSLVDLMNKSFPELGIKKTDCKELSWIDTTIFYSGVVNYNTANFKKEILLD-R 374

Query: 382 PLTPKRFFKAKSDYVNEPIPKAGLEGIWEFFNEEEAEAAVLILSPYGGIMDKISESEIPF 441
               K  F  K DYV + IP+  +  I E   EEE    + +L PYGGIMD+ISES IPF
Sbjct: 375 SAGKKTAFSIKLDYVKKLIPETAMVKILEKLYEEEVGVGMYVLYPYGGIMDEISESAIPF 434

Query: 442 PHRAGNLYKIQHLVYWDEEGEDIAKRHINWIRKLYSYMAPFVSKNPRAAYINYRDLDIGT 501
           PHRAG +Y++ +   W+++ ++  ++HINW+R +Y++  P+VS+NPR AY+NYRDLD+G 
Sbjct: 435 PHRAGIMYELWYTATWEKQEDN--EKHINWVRSVYNFTTPYVSQNPRLAYLNYRDLDLGK 494

Query: 502 NEKNGRNTSYNEASVWGVKYFKGNFKRLVSVKTKVDPSNFFKNEQSIPSLKP 548
                 N +Y +A +WG KYF  NF RLV VKTK DP+NFF+NEQSIP L P
Sbjct: 495 TNPESPN-NYTQARIWGEKYFGKNFNRLVKVKTKADPNNFFRNEQSIPPLPP 542

BLAST of CSPI01G24740 vs. Swiss-Prot
Match: CASL2_CANSA (Cannabidiolic acid synthase-like 2 OS=Cannabis sativa GN=CBDAS3 PE=2 SV=1)

HSP 1 Score: 545.8 bits (1405), Expect = 5.4e-154
Identity = 261/530 (49.25%), Postives = 369/530 (69.62%), Query Frame = 1

Query: 22  IFIVLTF-VWAASAHNHESFLHCLSHHSPNTSSISKIIYTPTNPSYSSVLNFSIHNLRFT 81
           IF  L+F +  + A+  E+FL CLS + P   + +K++YT  +  Y S+LN ++ NLRFT
Sbjct: 15  IFFFLSFNIQISIANPQENFLKCLSQYIPTNVTNAKLVYTQHDQFYMSILNSTVQNLRFT 74

Query: 82  SPKTPKPQVIVTPFHVSQIQASIICAKNTGFQIRTRSGGHDYEGLSYVSDVSFVVVDLIN 141
           S  TPKP VI TP +VS IQ +I+C+K  G QIRTRSGGHD EG+SY+S V FV+VDL N
Sbjct: 75  SDTTPKPLVITTPLNVSHIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLRN 134

Query: 142 LRSISVDAENNVAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHFSGGGYGLML 201
           + S+ +D  +  AWV+SGAT+GE+YY I E ++ L FPAG CPTVG GGHFSGGGYG ++
Sbjct: 135 MHSVKIDVHSQTAWVESGATLGEVYYWINENNENLSFPAGYCPTVGTGGHFSGGGYGALM 194

Query: 202 RKFGLAADNVIDAYFIDVNGKLHDRKSMGEDVFWAIRGSGGASYGIVLAWKIKLLPVPPI 261
           R +GLAADN+IDA+ ++V+GK+ DRKSMGED+FWAIRG GG ++GI+ AWKI+L+ VP +
Sbjct: 195 RNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVPSM 254

Query: 262 VTVFTIARTLE-QNATDIIHRWQYVSSKQDDKLFIRIILTGINTTNSQHGNKRTIEAAFN 321
            T+F++ + +E      ++++WQ ++   + +L +       N T++Q  NK TI + F+
Sbjct: 255 STIFSVKKNMEIHELVKLVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTTIHSYFS 314

Query: 322 SLFLGKIEELVPIMQKTFPELGLTKEDCIEMSWIESVLYFAGFSRGQPLN----VLLDRR 381
           S+F G ++ LV +M K+FPELG+ K DC ++SWI+++++++G       N    +LLDR 
Sbjct: 315 SIFHGGVDSLVDLMNKSFPELGIKKTDCKQLSWIDTIIFYSGVVNYNTTNFKKEILLDRS 374

Query: 382 PLTPKRFFKAKSDYVNEPIPKAGLEGIWEFFNEEEAEAAVLILSPYGGIMDKISESEIPF 441
               K  F  K DYV +PIP+  +  I E   EE+    + +  PYGGIMD+ISES IPF
Sbjct: 375 G-GRKAAFSIKLDYVKKPIPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISESAIPF 434

Query: 442 PHRAGNLYKIQHLVYWDEEGEDIAKRHINWIRKLYSYMAPFVSKNPRAAYINYRDLDIGT 501
           PHRAG  Y+I ++  W+++ ++  ++HINWIR +Y++  P+VS+NPR AY+NYRDLD+G 
Sbjct: 435 PHRAGITYEIWYIASWEKQEDN--EKHINWIRNVYNFTTPYVSQNPRMAYLNYRDLDLGK 494

Query: 502 NEKNGRNTSYNEASVWGVKYFKGNFKRLVSVKTKVDPSNFFKNEQSIPSL 546
                 N +Y +A +WG KYF  NF RLV VKTKVDP NFF+NEQSIP L
Sbjct: 495 TNFESPN-NYTQARIWGEKYFGKNFNRLVKVKTKVDPDNFFRNEQSIPPL 540

BLAST of CSPI01G24740 vs. Swiss-Prot
Match: CASL1_CANSA (Cannabidiolic acid synthase-like 1 OS=Cannabis sativa GN=CBDAS2 PE=2 SV=1)

HSP 1 Score: 543.5 bits (1399), Expect = 2.7e-153
Identity = 259/530 (48.87%), Postives = 369/530 (69.62%), Query Frame = 1

Query: 22  IFIVLTF-VWAASAHNHESFLHCLSHHSPNTSSISKIIYTPTNPSYSSVLNFSIHNLRFT 81
           IF  L+F +  + A+  E+FL C S + P   + +K++YT  +  Y S+LN +I NLRFT
Sbjct: 15  IFFFLSFNIQISIANPQENFLKCFSQYIPTNVTNAKLVYTQHDQFYMSILNSTIQNLRFT 74

Query: 82  SPKTPKPQVIVTPFHVSQIQASIICAKNTGFQIRTRSGGHDYEGLSYVSDVSFVVVDLIN 141
           S  TPKP VI+TP +VS IQ +I+C+K  G QIRTRSGGHD EG+SY+S V FV+VDL N
Sbjct: 75  SDTTPKPLVIITPLNVSHIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLRN 134

Query: 142 LRSISVDAENNVAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHFSGGGYGLML 201
           + S+ +D  +  AWV++GAT+GE+YY I E ++ L FPAG CPTVG GGHFSGGGYG ++
Sbjct: 135 MHSVKIDVHSQTAWVEAGATLGEVYYWINENNENLSFPAGYCPTVGAGGHFSGGGYGALM 194

Query: 202 RKFGLAADNVIDAYFIDVNGKLHDRKSMGEDVFWAIRGSGGASYGIVLAWKIKLLPVPPI 261
           R +GLAADN+IDA+ ++V+GK+ DRKSMGED+FWAIRG GG ++GI+ AWKI+L+ VP +
Sbjct: 195 RNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVPSM 254

Query: 262 VTVFTIARTLE-QNATDIIHRWQYVSSKQDDKLFIRIILTGINTTNSQHGNKRTIEAAFN 321
            T+F++ + +E      ++++WQ ++   + +L +       N T++Q  NK TI + F+
Sbjct: 255 STIFSVKKNMEIHELVKLVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTTIHSYFS 314

Query: 322 SLFLGKIEELVPIMQKTFPELGLTKEDCIEMSWIESVLYFAGFSRGQPL----NVLLDRR 381
           S+F G ++ LV +M K+FPELG+ K DC ++SWI+++++++G            +LLDR 
Sbjct: 315 SIFHGGVDSLVDLMNKSFPELGIKKTDCKQLSWIDTIIFYSGVVNYNTTYFKKEILLDRS 374

Query: 382 PLTPKRFFKAKSDYVNEPIPKAGLEGIWEFFNEEEAEAAVLILSPYGGIMDKISESEIPF 441
               K  F  K DYV +PIP+  +  I E   EE+    + +  PYGGIMD+ISES IPF
Sbjct: 375 G-GRKAAFSIKLDYVKKPIPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISESAIPF 434

Query: 442 PHRAGNLYKIQHLVYWDEEGEDIAKRHINWIRKLYSYMAPFVSKNPRAAYINYRDLDIGT 501
           PHRAG +Y+I ++  W+++ ++  ++HINWIR +Y++  P+VS+NPR AY+NYRDLD+G 
Sbjct: 435 PHRAGIMYEIWYIASWEKQEDN--EKHINWIRNVYNFTTPYVSQNPRMAYLNYRDLDLGK 494

Query: 502 NEKNGRNTSYNEASVWGVKYFKGNFKRLVSVKTKVDPSNFFKNEQSIPSL 546
                 N +Y +A +WG KYF  NF RLV VKTKVDP NFF+NEQSIP L
Sbjct: 495 TNFESPN-NYTQARIWGEKYFGKNFNRLVKVKTKVDPDNFFRNEQSIPPL 540

BLAST of CSPI01G24740 vs. TrEMBL
Match: M1D3J5_SOLTU (Uncharacterized protein OS=Solanum tuberosum GN=PGSC0003DMG400031326 PE=4 SV=1)

HSP 1 Score: 743.4 bits (1918), Expect = 2.0e-211
Identity = 361/542 (66.61%), Postives = 440/542 (81.18%), Query Frame = 1

Query: 12  MKSLITSFAHIFIVLTFV-WAASAHNHESFLHCLSHHSPNTSSISKIIYTPTNPSYSSVL 71
           MK    SF  + +VL+   W+A A NHE F+ CLSH +  +S+I    YTP N S+ S+L
Sbjct: 1   MKISWFSFIFVLVVLSSASWSALADNHEEFIQCLSHSNQTSSNI----YTPNNSSFPSIL 60

Query: 72  NFSIHNLRFTSPKTPKPQVIVTPFHVSQIQASIICAKNTGFQIRTRSGGHDYEGLSYVSD 131
            FSI NLRF + +TPKP VIVTP   S++Q  I+C+K TG  +R R  GHDYEGLSYVS+
Sbjct: 61  QFSIQNLRFNTTETPKPLVIVTPVSESEVQRVILCSKKTGMHVRVRGAGHDYEGLSYVSE 120

Query: 132 VSFVVVDLINLRSISVDAENNVAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGH 191
           V FV+VDLINLR+I+VD  +  AWV++G+TIGELYYRIAEKSKTLGFPAGVCPTVGVGGH
Sbjct: 121 VPFVIVDLINLRTINVDVNDKSAWVEAGSTIGELYYRIAEKSKTLGFPAGVCPTVGVGGH 180

Query: 192 FSGGGYGLMLRKFGLAADNVIDAYFIDVNGKLHDRKSMGEDVFWAIRGSGGASYGIVLAW 251
           FSGGGYG+MLRK+GLAADN++DA  ID NG++ DR SMGED+FWAIRG GG ++G+VLAW
Sbjct: 181 FSGGGYGVMLRKYGLAADNIVDARLIDANGRILDRVSMGEDLFWAIRGGGGNTFGLVLAW 240

Query: 252 KIKLLPVPPIVTVFTIARTLEQNATDIIHRWQYVSSKQDDKLFIRIILTGINTTNSQHG- 311
           KIKL+ VP IVTVFT+ +TLEQNAT ++HRWQYV+ +  + LFIRI+++ +N++N   G 
Sbjct: 241 KIKLVDVPEIVTVFTLDKTLEQNATKLVHRWQYVAPRFHEDLFIRILVSKLNSSNQGDGN 300

Query: 312 NKRTIEAAFNSLFLGKIEELVPIMQKTFPELGLTKEDCIEMSWIESVLYFAGFSRGQPLN 371
           N++TI A+FNS+FLG I+ L+PIM++ FPELGL +EDCIEMSWIES++YFAGF    PL+
Sbjct: 301 NQQTIVASFNSIFLGGIDRLLPIMKENFPELGLRREDCIEMSWIESIMYFAGFPTDGPLD 360

Query: 372 VLLDRRPLTPKRFFKAKSDYVNEPIPKAGLEGIWEFFNEEEAEAAVLILSPYGGIMDKIS 431
           VLL R  L+  R+FKAKSDYV +PIP+ GLEGIW FF E+EA+++ +ILSPYGG MD+IS
Sbjct: 361 VLLSRVQLS-TRYFKAKSDYVYQPIPEGGLEGIWRFFFEDEAQSSQVILSPYGGRMDEIS 420

Query: 432 ESEIPFPHRAGNLYKIQHLVYWDEEGEDIAKRHINWIRKLYSYMAPFVSKNPRAAYINYR 491
            S IPFPHRAGNLYKIQHLVYWDEEGE++A+RHI+WIR+LYSYMAPFVSK PRAAYINYR
Sbjct: 421 PSAIPFPHRAGNLYKIQHLVYWDEEGEEVAERHISWIRRLYSYMAPFVSKLPRAAYINYR 480

Query: 492 DLDIGTNEKNGRNTSYNEASVWGVKYFKGNFKRLVSVKTKVDPSNFFKNEQSIPSLKPWW 551
           DLDIG N   G  TSY +A VWG+KYFK NF RLV VKTKVDPSNFF+NEQSIPSL  W 
Sbjct: 481 DLDIGVNNIKG-YTSYVQAKVWGIKYFKNNFDRLVHVKTKVDPSNFFRNEQSIPSLTQWK 536

BLAST of CSPI01G24740 vs. TrEMBL
Match: K4C7D5_SOLLC (Uncharacterized protein OS=Solanum lycopersicum PE=4 SV=1)

HSP 1 Score: 742.7 bits (1916), Expect = 3.4e-211
Identity = 358/540 (66.30%), Postives = 438/540 (81.11%), Query Frame = 1

Query: 16  ITSFAHIFIVLTFV---WAASAHNHESFLHCLSHHSPNTSSISKIIYTPTNPSYSSVLNF 75
           I+ F+ IF++L      W+  A NHE F+ CLSH++  +S+I    YTP N S+ S+L F
Sbjct: 3   ISWFSFIFVLLVLSSASWSTLADNHEEFIQCLSHNNQTSSNI----YTPNNSSFQSILQF 62

Query: 76  SIHNLRFTSPKTPKPQVIVTPFHVSQIQASIICAKNTGFQIRTRSGGHDYEGLSYVSDVS 135
           SI NLRF + +TPKP VIVTP   S++Q  I+CAK TG  +R R  GHDYEGLSYVS+V 
Sbjct: 63  SIQNLRFNTSETPKPLVIVTPVSESEVQRVILCAKKTGMHVRVRGAGHDYEGLSYVSEVP 122

Query: 136 FVVVDLINLRSISVDAENNVAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHFS 195
           FV+VDLINLR+I+VD  +  AWV++G+TIGELYYRIAEKSKTLGFPAGVCPTVGVGGHFS
Sbjct: 123 FVIVDLINLRTINVDVNDKSAWVEAGSTIGELYYRIAEKSKTLGFPAGVCPTVGVGGHFS 182

Query: 196 GGGYGLMLRKFGLAADNVIDAYFIDVNGKLHDRKSMGEDVFWAIRGSGGASYGIVLAWKI 255
           GGGYG+MLRK+GLAADN++DA  ID NG++ DR SMGED+FWAIRG GG S+G+VLAWK+
Sbjct: 183 GGGYGVMLRKYGLAADNIVDARLIDANGRILDRASMGEDLFWAIRGGGGNSFGLVLAWKV 242

Query: 256 KLLPVPPIVTVFTIARTLEQNATDIIHRWQYVSSKQDDKLFIRIILTGINTTN-SQHGNK 315
           KL+ VP IVTVFT+ +TLEQNAT ++H+WQYV+ +    LFIRI+++ +N++N     N+
Sbjct: 243 KLVDVPEIVTVFTLDKTLEQNATKLVHKWQYVAPRFHQDLFIRILVSRLNSSNQGDDNNQ 302

Query: 316 RTIEAAFNSLFLGKIEELVPIMQKTFPELGLTKEDCIEMSWIESVLYFAGFSRGQPLNVL 375
           +TI A+FNS+FLG I+ L+PIMQ+ FPELGL + DCIEMSWIES+LYFAGF    PL+VL
Sbjct: 303 QTIVASFNSIFLGGIDRLLPIMQENFPELGLRRADCIEMSWIESILYFAGFPTDGPLDVL 362

Query: 376 LDRRPLTPKRFFKAKSDYVNEPIPKAGLEGIWEFFNEEEAEAAVLILSPYGGIMDKISES 435
           L R  L+  R+FKAKSDYV +PIP+ GL+GIW FF E+EA+++ +ILSPYGG MD+IS S
Sbjct: 363 LSRVQLS-TRYFKAKSDYVYQPIPEGGLDGIWRFFFEDEAQSSQVILSPYGGRMDEISPS 422

Query: 436 EIPFPHRAGNLYKIQHLVYWDEEGEDIAKRHINWIRKLYSYMAPFVSKNPRAAYINYRDL 495
            IPFPHRAGNLYKIQHLVYWDEEGE++A+RHI+WIR+LYSYMAPFVSK+PRAAYINYRDL
Sbjct: 423 AIPFPHRAGNLYKIQHLVYWDEEGEEVAERHISWIRRLYSYMAPFVSKSPRAAYINYRDL 482

Query: 496 DIGTNEKNGRNTSYNEASVWGVKYFKGNFKRLVSVKTKVDPSNFFKNEQSIPSLKPWWNK 552
           DIG N   G  TSY +A VWG+KYFK NF RLV VKTKVDPSNFF+NEQSIPSL  W NK
Sbjct: 483 DIGVNNIKG-YTSYVQAKVWGIKYFKNNFDRLVHVKTKVDPSNFFRNEQSIPSLTQWKNK 536

BLAST of CSPI01G24740 vs. TrEMBL
Match: B9NA97_POPTR (FAD-binding domain-containing family protein OS=Populus trichocarpa GN=POPTR_0001s43120g PE=4 SV=1)

HSP 1 Score: 740.3 bits (1910), Expect = 1.7e-210
Identity = 353/526 (67.11%), Postives = 424/526 (80.61%), Query Frame = 1

Query: 23  FIVLTFVWAASAHNHESFLHCLSHHSPNTSSISKIIYTPTNPSYSSVLNFSIHNLRFTSP 82
           F++ +F W  SAH HESFL CL   S N+ SISK+IYTP N SYSS+L FSI NLRF + 
Sbjct: 7   FLIFSFSWVTSAHTHESFLQCLD--SQNSHSISKLIYTPINSSYSSILQFSIQNLRFNTS 66

Query: 83  KTPKPQVIVTPFHVSQIQASIICAKNTGFQIRTRSGGHDYEGLSYVSDVSFVVVDLINLR 142
            TPKP VIVTP ++S IQA+IIC++  G QIR RSGGHDYEGLSYVS  SFVV+DLINLR
Sbjct: 67  STPKPLVIVTPTNISHIQAAIICSQKHGMQIRIRSGGHDYEGLSYVSTFSFVVIDLINLR 126

Query: 143 SISVDAENNVAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHFSGGGYGLMLRK 202
           +I+V+AEN  AWVQSG TIGE+YYRIAEKS+TL FPA VCPTVGVGGHFSGGGYG+++RK
Sbjct: 127 TINVNAENKTAWVQSGVTIGEVYYRIAEKSRTLAFPASVCPTVGVGGHFSGGGYGMLMRK 186

Query: 203 FGLAADNVIDAYFIDVNGKLHDRKSMGEDVFWAIRGSGGASYGIVLAWKIKLLPVPPIVT 262
           +GLAAD++IDA  +DV G++ DR+SMGED+FWAIRG GG ++G+V+AWKI L+  PP VT
Sbjct: 187 YGLAADHIIDAQLVDVKGRILDRESMGEDLFWAIRGGGGNTFGVVIAWKISLVSAPPTVT 246

Query: 263 VFTIARTLEQNATDIIHRWQYVSSKQDDKLFIRIILTGINTTNSQHGNKRTIEAAFNSLF 322
           VF + RTLEQNA+ ++HRWQ+V+ K  + LFIRIIL  +N+T     N  TI+A+FNSLF
Sbjct: 247 VFNVQRTLEQNASKLVHRWQFVADKLHEDLFIRIILNRVNSTEEGKSN-TTIQASFNSLF 306

Query: 323 LGKIEELVPIMQKTFPELGLTKEDCIEMSWIESVLYFAGFSRGQPLNVLLDRRPLTPKRF 382
           LG I+ L+P++Q +FPELGL KEDCIEMSWI+SVLYF GF     L+VLLDR P T +R 
Sbjct: 307 LGGIDRLLPLIQDSFPELGLVKEDCIEMSWIQSVLYFDGFPSNSSLDVLLDRTPST-RRN 366

Query: 383 FKAKSDYVNEPIPKAGLEGIWEFFNEEEAEAAVLILSPYGGIMDKISESEIPFPHRAGNL 442
           FKAKSDYV EPIP+ GLEGIWE F +++    +LI SPYGG M +ISES IPFPHRAGN+
Sbjct: 367 FKAKSDYVKEPIPELGLEGIWERFFDKDINTPILIFSPYGGKMSEISESSIPFPHRAGNI 426

Query: 443 YKIQHLVYWDEEGEDIAKRHINWIRKLYSYMAPFVSKNPRAAYINYRDLDIGTNEKNGRN 502
           YKIQHL+YWDEEG    KRHI+WIR+LYSY+AP+VSK PRAAY+NYRDLDIG N   G N
Sbjct: 427 YKIQHLIYWDEEGIVATKRHISWIRRLYSYLAPYVSKTPRAAYVNYRDLDIGINNHAG-N 486

Query: 503 TSYNEASVWGVKYFKGNFKRLVSVKTKVDPSNFFKNEQSIPSLKPW 549
           TSY +AS+WG+KYFK NF RLV VKT VDP+NFF+NEQSIP L  W
Sbjct: 487 TSYRQASIWGLKYFKNNFDRLVRVKTAVDPANFFRNEQSIPPLSSW 527

BLAST of CSPI01G24740 vs. TrEMBL
Match: M1D3J6_SOLTU (Uncharacterized protein OS=Solanum tuberosum GN=PGSC0003DMG400031327 PE=4 SV=1)

HSP 1 Score: 709.9 bits (1831), Expect = 2.4e-201
Identity = 346/536 (64.55%), Postives = 426/536 (79.48%), Query Frame = 1

Query: 12  MKSLITSFAHIFIVLTFV-WAASAHNHESFLHCLSHHSPNTSSISKIIYTPTNPSYSSVL 71
           MK  + SF  + +VL+   W+  A NHE+F+ CLS  +       K  YTP N S+SS+L
Sbjct: 1   MKISLFSFLLLLLVLSSSSWSTLADNHEAFIQCLSQSN-------KTYYTPNNSSFSSIL 60

Query: 72  NFSIHNLRFTSPKTPKPQVIVTPFHVSQIQASIICAKNTGFQIRTRSGGHDYEGLSYVSD 131
            FSI NL F + +TPKP VIVTP   S++Q  I+CAK TG  +R RS GHDYEGLSYVS+
Sbjct: 61  QFSIQNLMFNTTETPKPLVIVTPVSESEVQRVILCAKKTGMHVRVRSAGHDYEGLSYVSE 120

Query: 132 VSFVVVDLINLRSISVDAENNVAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGH 191
           V FV+VDLINLR+I+VD  +  AWV++G+TIGELYYRIAEKSKTLGFPAGVCPTVGVGGH
Sbjct: 121 VPFVIVDLINLRTINVDVNDKSAWVEAGSTIGELYYRIAEKSKTLGFPAGVCPTVGVGGH 180

Query: 192 FSGGGYGLMLRKFGLAADNVIDAYFIDVNGKLHDRKSMGEDVFWAIRGSGGASYGIVLAW 251
           FSGGGYG M+RK+GL+ADN++DA  +D NG++ DR SMGED+FWAIRG GG ++G+VL+W
Sbjct: 181 FSGGGYGTMMRKYGLSADNIVDARLVDANGRILDRVSMGEDLFWAIRGGGGNTFGLVLSW 240

Query: 252 KIKLLPVPPIVTVFTIARTLEQNATDIIHRWQYVSSKQDDKLFIRIILTGINTTNSQHGN 311
           KIKLL VP  VTVFT+ +TLEQN T +IH+WQ V+ +  + LFIRII+  +++++    N
Sbjct: 241 KIKLLDVPEKVTVFTLDKTLEQNLTKLIHKWQNVAPRFHEDLFIRIIIRRVDSSDQGGNN 300

Query: 312 KRTIEAAFNSLFLGKIEELVPIMQKTFPELGLTKEDCIEMSWIESVLYFAGFSRGQPLNV 371
           K+TI A+FNSLF G I+ L+PIMQ+ FPELGL +EDCIEMSWIES++YFAGF      +V
Sbjct: 301 KQTIVASFNSLFFGGIDRLLPIMQENFPELGLRREDCIEMSWIESIMYFAGFPIEGSFDV 360

Query: 372 LLDR-RPLTPKRFFKAKSDYVNEPIPKAGLEGIWEFFNEEEAEAAVLILSPYGGIMDKIS 431
           LL R +P T  R+FKAKSDYV +PIP+ GLEGIW FF E+EA+++ +IL+PYGG MD+I 
Sbjct: 361 LLSRVQPTT--RYFKAKSDYVYQPIPEGGLEGIWRFFFEDEAQSSYMILTPYGGRMDEIL 420

Query: 432 ESEIPFPHRAGNLYKIQHLVYWDEEGEDIAKRHINWIRKLYSYMAPFVSKNPRAAYINYR 491
            S+IPFPHRAGNLYKIQHLVYWD+EGE++A+RHI+WIR+LYSYMAPFVS  PRAAY+NYR
Sbjct: 421 PSDIPFPHRAGNLYKIQHLVYWDKEGEEVAERHISWIRRLYSYMAPFVSMFPRAAYVNYR 480

Query: 492 DLDIGTNEKNGRNTSYNEASVWGVKYFKGNFKRLVSVKTKVDPSNFFKNEQSIPSL 546
           DLDIG N K G  TSY +A VWG+KYFK NF RLV VKTKVDPSNFF NEQSIPSL
Sbjct: 481 DLDIGMNNKKG-YTSYMQAKVWGMKYFKNNFDRLVHVKTKVDPSNFFWNEQSIPSL 526

BLAST of CSPI01G24740 vs. TrEMBL
Match: K4C7D6_SOLLC (Uncharacterized protein OS=Solanum lycopersicum PE=4 SV=1)

HSP 1 Score: 709.9 bits (1831), Expect = 2.4e-201
Identity = 348/536 (64.93%), Postives = 427/536 (79.66%), Query Frame = 1

Query: 12  MKSLITSFAHIFIVLTFV-WAASAHNHESFLHCLSHHSPNTSSISKIIYTPTNPSYSSVL 71
           MK  + SF  + +VL+    +A A NHE F+ CLSH++       +  YTP N S+SS+L
Sbjct: 1   MKISLFSFLLLLLVLSSSSLSALADNHEVFIQCLSHNN-------QTYYTPNNSSFSSIL 60

Query: 72  NFSIHNLRFTSPKTPKPQVIVTPFHVSQIQASIICAKNTGFQIRTRSGGHDYEGLSYVSD 131
            FSI NLRF + +TPKP VIVTP   S++Q  I+CAK  G  +R RS GHDYEGLSYVS+
Sbjct: 61  QFSIQNLRFNTSETPKPLVIVTPVSESEVQRVILCAKKIGMHVRVRSAGHDYEGLSYVSE 120

Query: 132 VSFVVVDLINLRSISVDAENNVAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGH 191
           V FV+VDLINLR+I+VD  +  AWV++G+TIGELYYRIAEKSKTLGFPAGVCPTVGVGGH
Sbjct: 121 VPFVIVDLINLRTINVDVNDKSAWVEAGSTIGELYYRIAEKSKTLGFPAGVCPTVGVGGH 180

Query: 192 FSGGGYGLMLRKFGLAADNVIDAYFIDVNGKLHDRKSMGEDVFWAIRGSGGASYGIVLAW 251
           FSGGGYG M+RK+GL+ADN++DA  +D NG + DR SMGED+FWAIRG GG ++G+VLAW
Sbjct: 181 FSGGGYGTMMRKYGLSADNIVDARLVDANGMILDRVSMGEDLFWAIRGGGGNTFGLVLAW 240

Query: 252 KIKLLPVPPIVTVFTIARTLEQNATDIIHRWQYVSSKQDDKLFIRIILTGINTTNSQHGN 311
           K+KLL VP  VTVFT+ +TLEQN T +IH+WQ V+ +    LFIR+I+  +++++    N
Sbjct: 241 KVKLLDVPEKVTVFTLDKTLEQNLTKLIHKWQNVAPRFHKDLFIRVIIRRVDSSDQGGNN 300

Query: 312 KRTIEAAFNSLFLGKIEELVPIMQKTFPELGLTKEDCIEMSWIESVLYFAGFSRGQPLNV 371
           K+TI A+FNSLF+G I+ L+PIMQ+ FPELGL KEDCIEMSWI+S++YFAGF     L+V
Sbjct: 301 KQTIVASFNSLFIGGIDRLLPIMQENFPELGLRKEDCIEMSWIQSIMYFAGFPIHGSLDV 360

Query: 372 LLDR-RPLTPKRFFKAKSDYVNEPIPKAGLEGIWEFFNEEEAEAAVLILSPYGGIMDKIS 431
           LL R +P T  R+FKAKSDYV +PIP+ GLEGIW FF EEEA+++ +IL+PYGG MD+IS
Sbjct: 361 LLSRVQPTT--RYFKAKSDYVYQPIPEGGLEGIWRFFFEEEAKSSYVILTPYGGRMDEIS 420

Query: 432 ESEIPFPHRAGNLYKIQHLVYWDEEGEDIAKRHINWIRKLYSYMAPFVSKNPRAAYINYR 491
            S IPFPHRAGNLYKIQHLVYWDEEGE++A+RHI+WIR+LYSYMAPFVSK PRAAYINYR
Sbjct: 421 PSAIPFPHRAGNLYKIQHLVYWDEEGEEVAERHISWIRRLYSYMAPFVSKFPRAAYINYR 480

Query: 492 DLDIGTNEKNGRNTSYNEASVWGVKYFKGNFKRLVSVKTKVDPSNFFKNEQSIPSL 546
           D+DIG N K G  TSY +A VWG+KYFK NF  LV VKTKVDP+NFF NEQSIPSL
Sbjct: 481 DIDIGMNNKKG-YTSYMQAKVWGIKYFKNNFDGLVHVKTKVDPTNFFWNEQSIPSL 526

BLAST of CSPI01G24740 vs. TAIR10
Match: AT1G30760.1 (AT1G30760.1 FAD-binding Berberine family protein)

HSP 1 Score: 555.1 bits (1429), Expect = 5.0e-158
Identity = 273/543 (50.28%), Postives = 376/543 (69.24%), Query Frame = 1

Query: 6   NKSTFKMKSLITSFAHIFIVLTFVWAASAHNHESFLHCLSHHSPNTSSISKIIYTP--TN 65
           N + F +  L+ S ++I +  + +        + F+ CL  +S  +  +    +T     
Sbjct: 8   NTNAFLVTLLLLSLSYIPLSFSTI-------QQDFVMCLVDNSDASFPMDSSFFTHDLNA 67

Query: 66  PSYSSVLNFSIHNLRFTSPKTPKPQVIVTPFHVSQIQASIICAKNTGFQIRTRSGGHDYE 125
            S+   L  S  NLR+  P  PKP+ I  P + + +QA+++CAK     +R RSGGHDYE
Sbjct: 68  SSFKLALETSAQNLRYLMPSNPKPEFIFEPLYETHVQAAVLCAKKLKLHLRLRSGGHDYE 127

Query: 126 GLSYVSDV--SFVVVDLINLRSISVDAENNVAWVQSGATIGELYYRIAEKSKTLGFPAGV 185
           GLSYVS++  +FV+VDL  LR ISVD E+N AWV +GA+IGE+YYRI EKSK  GFPAG+
Sbjct: 128 GLSYVSEMETAFVIVDLSKLRQISVDIESNSAWVHAGASIGEVYYRIQEKSKIHGFPAGL 187

Query: 186 CPTVGVGGHFSGGGYGLMLRKFGLAADNVIDAYFIDVNGKLHDRKSMGEDVFWAIRGSGG 245
           C ++G+GGH  GG YG M+RKFGL ADNV+DA  +D +GK+ +R +MGEDVFWAIRG GG
Sbjct: 188 CTSLGIGGHIIGGAYGSMMRKFGLGADNVLDARIVDADGKILNRAAMGEDVFWAIRGGGG 247

Query: 246 ASYGIVLAWKIKLLPVPPIVTVFTIARTLEQNATDIIHRWQYVSSKQDDKLFIRIILTGI 305
            S+G++LAWKIKL+PVP IVTVFT+ RTLEQ+ T ++++WQ V+ K D+ LFIR+I+   
Sbjct: 248 GSFGVILAWKIKLVPVPEIVTVFTVTRTLEQDGTKLLYKWQQVADKLDEDLFIRVIIQP- 307

Query: 306 NTTNSQHGNKRTIEAAFNSLFLGKIEELVPIMQKTFPELGLTKEDCIEMSWIESVLYFAG 365
            T+ +    +RTI  ++   FLG    L+ +MQ++FP+LGLTK+DC+E SWI+SV+Y AG
Sbjct: 308 -TSKTPKSKERTISTSYQGQFLGDANRLLQVMQRSFPQLGLTKKDCLETSWIKSVMYIAG 367

Query: 366 FSRGQPLNVLLDRRPLTPKRFFKAKSDYVNEPIPKAGLEGIWEFFNEEEAEAAVLILSPY 425
           F    P   LLD + L  K +FKAKSDYV EPIP  GLEG+WE   EE  ++ + I +PY
Sbjct: 368 FPSTAPSEALLDGKSLF-KNYFKAKSDYVEEPIPVEGLEGLWEKLLEE--DSPLTIWNPY 427

Query: 426 GGIMDKISESEIPFPHRAGNLYKIQHLVYWDEEGEDIAKRHINWIRKLYSYMAPFVSKNP 485
           GG+M KI E+E PFPHR+G L+KIQ L  W ++G+    +H+ W+R++YSYM  +VSK+P
Sbjct: 428 GGMMAKIPETETPFPHRSGTLFKIQWLTLW-QDGKTSEAKHMGWMREMYSYMEQYVSKSP 487

Query: 486 RAAYINYRDLDIGTNEKNGRNTSYNEASVWGVKYFKGNFKRLVSVKTKVDPSNFFKNEQS 545
           R+AY+NYRDLD+G N K       ++A  WG +YFKGNF+RLV +K K DP NFF++EQS
Sbjct: 488 RSAYVNYRDLDLGMNGKG------SDAREWGNRYFKGNFERLVEIKAKFDPENFFRHEQS 531

BLAST of CSPI01G24740 vs. TAIR10
Match: AT2G34790.1 (AT2G34790.1 FAD-binding Berberine family protein)

HSP 1 Score: 553.5 bits (1425), Expect = 1.5e-157
Identity = 270/527 (51.23%), Postives = 372/527 (70.59%), Query Frame = 1

Query: 22  IFIVLTFVWAASAHNHESFLHCLSHHSPNTSSISKIIYTPTNPS--YSSVLNFSIHNLRF 81
           + ++L  V  +S+   + F+ CL  +S  +  I+   ++P   +  +   L  +  NLR+
Sbjct: 15  VTLLLISVPLSSSTLQQDFVKCLVDNSDVSFPITASFFSPDQNATLFKEELESTAQNLRY 74

Query: 82  TSPKTPKPQVIVTPFHVSQIQASIICAKNTGFQIRTRSGGHDYEGLSYVSD--VSFVVVD 141
            +P  PKP  I  P + + +QA+++CAK     +R RSGGHDYEGLS+V++    FV+VD
Sbjct: 75  LTPSNPKPVFIFEPLYETHVQAAVVCAKKLQLHLRLRSGGHDYEGLSFVAEDETPFVIVD 134

Query: 142 LINLRSISVDAENNVAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHFSGGGYG 201
           L  LR + VD ++N AW  +GATIGE+YYRI EKS+T GFPAG+C ++G+GGH  GG YG
Sbjct: 135 LSKLRQVDVDLDSNSAWAHAGATIGEVYYRIQEKSQTHGFPAGLCSSLGIGGHLVGGAYG 194

Query: 202 LMLRKFGLAADNVIDAYFIDVNGKLHDRKSMGEDVFWAIRGSGGASYGIVLAWKIKLLPV 261
            M+RKFGL ADNV+DA  +D NG++ DR +MGEDVFWAIRG GG S+G++LAWKIKL+PV
Sbjct: 195 SMMRKFGLGADNVLDARIVDANGQILDRAAMGEDVFWAIRGGGGGSFGVILAWKIKLVPV 254

Query: 262 PPIVTVFTIARTLEQNATDIIHRWQYVSSKQDDKLFIRIILTGINTTNSQHGNKRTIEAA 321
           P  VTVFT+ +TLEQ+ T ++++W+ ++ K DD LFIR+I++  + T ++ GN RTI  +
Sbjct: 255 PATVTVFTVTKTLEQDGTKVLYKWEQIADKLDDDLFIRVIISPASKT-TKPGN-RTISMS 314

Query: 322 FNSLFLGKIEELVPIMQKTFPELGLTKEDCIEMSWIESVLYFAGFSRGQPLNVLLDRRPL 381
           + + FLG    L+ +MQK+FPELGLTK+DC EMSWI+SV+Y AGF        LL  + L
Sbjct: 315 YQAQFLGDSNRLLQVMQKSFPELGLTKKDCTEMSWIKSVMYIAGFPNSAAPEALLAGKSL 374

Query: 382 TPKRFFKAKSDYVNEPIPKAGLEGIWEFFNEEEAEAAVLILSPYGGIMDKISESEIPFPH 441
             K  FKAKSD+V EPIP  GLEG+WE F EE  ++ + I +PYGG+M +ISESEIPFPH
Sbjct: 375 F-KNHFKAKSDFVKEPIPVEGLEGLWERFLEE--DSPLTIWNPYGGMMSRISESEIPFPH 434

Query: 442 RAGNLYKIQHLVYWDEEGEDIAKRHINWIRKLYSYMAPFVSKNPRAAYINYRDLDIGTNE 501
           R G L+KIQ L  W ++G+   +RH+ WIR++YSYM  +VSKNPR AY+NYRDLD+GTNE
Sbjct: 435 RNGTLFKIQWLSTW-QDGKVSEERHMKWIREMYSYMEQYVSKNPRQAYVNYRDLDLGTNE 494

Query: 502 KNGRNTSYNEASVWGVKYFKGNFKRLVSVKTKVDPSNFFKNEQSIPS 545
                    +A  WG KY+KGNF+RLV +K + DP NFF++EQS+P+
Sbjct: 495 ------GETDAREWGAKYYKGNFERLVKIKGEFDPDNFFRHEQSVPT 529

BLAST of CSPI01G24740 vs. TAIR10
Match: AT4G20820.1 (AT4G20820.1 FAD-binding Berberine family protein)

HSP 1 Score: 553.5 bits (1425), Expect = 1.5e-157
Identity = 275/539 (51.02%), Postives = 372/539 (69.02%), Query Frame = 1

Query: 5   KNKSTFKMKSLITSFAHIFIVLTFVWAASAHNHESFLHCLSHHSPNTSSISKIIYTPTNP 64
           K +S F    +  + + + + +    +A+  N  SFL CLS    +++ +SK+I+TP + 
Sbjct: 2   KFQSFFSSVLIFFTTSTLLLSIPHPVSANRSNQSSFLQCLSLQLNDSNIVSKVIHTPNDT 61

Query: 65  SYSSVLNFSIHNLRFTSPKTPKPQVIVTPFHVSQIQASIICAKNTGFQIRTRSGGHDYEG 124
           S+SSVL  SI N RF++P  PKP +I+TP   S +Q+++ CA+  G  IRTRSGGHDYEG
Sbjct: 62  SFSSVLASSIQNQRFSAPDVPKPVLILTPVQPSDVQSAVKCARRFGIHIRTRSGGHDYEG 121

Query: 125 LSYVSDVSFVVVDLINLRSISVDAENNVAWVQSGATIGELYYRIAEKSKTLGFPAGVCPT 184
           LSYV+   FV++DL NLRSI+VD +N   WVQ+GATIGELYY I +K++TL FPAGVCPT
Sbjct: 122 LSYVTHKPFVILDLRNLRSITVDVDNRSVWVQTGATIGELYYEIGKKNRTLAFPAGVCPT 181

Query: 185 VGVGGHFSGGGYGLMLRKFGLAADNVIDAYFIDVNGKLHDRKSMGEDVFWAIRGSGGASY 244
           VGVGGHFSGGGYG +LRK GLAAD+VIDA  +D  G++ +R+ MGED FWAIRG GG+S+
Sbjct: 182 VGVGGHFSGGGYGTLLRKHGLAADHVIDARVVDARGRILERREMGEDFFWAIRGGGGSSF 241

Query: 245 GIVLAWKIKLLPVPPIVTVFTIARTLEQNATDIIHRWQYVSSKQDDKLFIRIILTGINTT 304
            +VL+WKI L+ VP  VTVF + +  EQ+A  IIHRWQ+V+ K  D LFIR++L      
Sbjct: 242 CVVLSWKIGLINVPSTVTVFNVTKFSEQSALKIIHRWQFVADKVSDDLFIRVML------ 301

Query: 305 NSQHGNKRTIEAAFNSLFLGKIEELVPIMQKTFPELGLTKEDCIEMSWIESVLYFAGFSR 364
                 K  + A+F  L+LG ++ L+ ++ K FPELGL ++DC EMSWIESV++FA    
Sbjct: 302 ---QRYKNMVRASFPGLYLGSVKNLLKMVNKEFPELGLEEDDCTEMSWIESVIWFAELGE 361

Query: 365 GQPLNVLLDRRPLTPKRFFKAKSDYVNEPIPKAGLEGIWEFFNEEEAEAAVLILSPYGGI 424
            +P+NVL  R        FKAKSD+V EP+PK  +  +W    E EAE A LI +P+GG 
Sbjct: 362 -EPINVLTKR--TRASLAFKAKSDFVQEPMPKTAISKLWRRLQEPEAEHAQLIFTPFGGK 421

Query: 425 MDKISESEIPFPHRAGNLYKIQHLVYWDEEGEDIAKRHINWIRKLYSYMAPFVSKNPRAA 484
           M +I++ E PFPHR GN+Y+IQ+L YW     D+ ++++ W+ ++Y  M+ FV+K+PR A
Sbjct: 422 MSEIADYETPFPHRKGNIYEIQYLNYW---RGDVKEKYMRWVERVYDDMSEFVAKSPRGA 481

Query: 485 YINYRDLDIGTNEKNGRNTSYNEASVWGVKYFKGNFKRLVSVKTKVDPSNFFKNEQSIP 544
           YIN RDLD+G      + + Y E   WGVKYFK NF+RLV VKT VDPS+FF +EQSIP
Sbjct: 482 YINLRDLDLGM-YVGVKRSKYEEGKSWGVKYFKNNFERLVRVKTSVDPSDFFCDEQSIP 524

BLAST of CSPI01G24740 vs. TAIR10
Match: AT5G44400.1 (AT5G44400.1 FAD-binding Berberine family protein)

HSP 1 Score: 552.0 bits (1421), Expect = 4.2e-157
Identity = 267/513 (52.05%), Postives = 360/513 (70.18%), Query Frame = 1

Query: 38  ESFLHCLSHHSPNTSSISKIIYTPTN--PSYSSVLNFSIHNLRFTSPKTPKPQVIVTPFH 97
           + F++C+  ++     + K  + PT     +S VL  +  NLRF     PKP  I +P H
Sbjct: 33  DQFINCVQRNTHVYFPLEKTFFAPTKNVSMFSQVLESTAQNLRFLKKSMPKPGFIFSPIH 92

Query: 98  VSQIQASIICAKNTGFQIRTRSGGHDYEGLSYVSDVS--FVVVDLINLRSISVDAENNVA 157
            S +QASIIC+K     +R RSGGHDYEGLSYVS +   F+++DL  +R ++++ ++N A
Sbjct: 93  ESHVQASIICSKKLRMHLRVRSGGHDYEGLSYVSQIDKPFILMDLSKMRQVNINIQDNSA 152

Query: 158 WVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHFSGGGYGLMLRKFGLAADNVIDA 217
           WVQSGAT+GELYYRIAEKSK  GFPAG+C ++G+GGH +GG YG M+RK+GL ADNV+DA
Sbjct: 153 WVQSGATVGELYYRIAEKSKVHGFPAGLCSSLGIGGHITGGAYGSMMRKYGLGADNVLDA 212

Query: 218 YFIDVNGKLHDRKSMGEDVFWAIRGSGGASYGIVLAWKIKLLPVPPIVTVFTIARTLEQN 277
             +D NGKL DR +MGED FWAIRG  G S+GI+LAWKIKL+PVP  VTVFT+ +TL+Q+
Sbjct: 213 KIVDANGKLLDRAAMGEDTFWAIRGGAGGSFGIILAWKIKLVPVPKTVTVFTVTKTLQQD 272

Query: 278 -ATDIIHRWQYVSSKQDDKLFIRIILTGINTTNSQHGNKRTIEAAFNSLFLGKIEELVPI 337
               II +WQ V+ K  ++LFIR++     T     G  +T+  ++N+LFLG    L+ +
Sbjct: 273 VGNKIISKWQRVADKLVEELFIRVLFNVAGT-----GGNKTVTTSYNALFLGGKGTLMNV 332

Query: 338 MQKTFPELGLTKEDCIEMSWIESVLYFAGFSRGQPLNVLLDRRPLTPKRFFKAKSDYVNE 397
           M+K+FPELGLT +DCIEMSW+ES+ Y +GF    P NVLL  +   PK  FKAKSD+V  
Sbjct: 333 MKKSFPELGLTFKDCIEMSWLESIAYISGFPTHTPTNVLLQGKSPFPKVSFKAKSDFVKT 392

Query: 398 PIPKAGLEGIWEFFNEEEAEAAVLILSPYGGIMDKISESEIPFPHRAGNLYKIQHLVYWD 457
           PIP++GL+GI++   +E  +  ++I +PYGG+M KI ES+IPFPHR G L+K+Q++  W 
Sbjct: 393 PIPESGLQGIFKKLLKE--DIPLMIWNPYGGMMAKIPESQIPFPHRKGVLFKVQYVTSW- 452

Query: 458 EEGEDIAKRHINWIRKLYSYMAPFVSKNPRAAYINYRDLDIGTNEKNGRNTSYNEASVWG 517
            + +    RHINWIR LYSYM P+VS NPR AY+NYRDLD+G N K+ + T   +A VWG
Sbjct: 453 LDSDKRPSRHINWIRDLYSYMTPYVSSNPREAYVNYRDLDLGRNTKDVK-TCIKQAQVWG 512

Query: 518 VKYFKGNFKRLVSVKTKVDPSNFFKNEQSIPSL 546
             YFK NF RL+ +K KVDP NFF++EQSIP +
Sbjct: 513 ANYFKNNFNRLMMIKAKVDPENFFRHEQSIPPM 536

BLAST of CSPI01G24740 vs. TAIR10
Match: AT1G30700.1 (AT1G30700.1 FAD-binding Berberine family protein)

HSP 1 Score: 550.1 bits (1416), Expect = 1.6e-156
Identity = 276/528 (52.27%), Postives = 359/528 (67.99%), Query Frame = 1

Query: 22  IFIVLTFVW--AASAHNHESFLHCLSHHSPNTSSISKIIYTPTNPSYSSVLNFSIHNLRF 81
           +   + F+W  ++S+ N E+F  CL+ +S     IS  I+   N SYSSVL  +I NLRF
Sbjct: 8   VLFFVVFIWQSSSSSANSETFTQCLTSNSDPKHPISPAIFFSGNGSYSSVLQANIRNLRF 67

Query: 82  TSPKTPKPQVIVTPFHVSQIQASIICAKNTGFQIRTRSGGHDYEGLSYV--SDVSFVVVD 141
            +  TPKP +I+   H S +QA+I C K    Q++ RSGGHDY+GLSYV  S   F V+D
Sbjct: 68  NTTSTPKPFLIIAATHESHVQAAITCGKRHNLQMKIRSGGHDYDGLSYVTYSGKPFFVLD 127

Query: 142 LINLRSISVDAENNVAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHFSGGGYG 201
           + NLRS+ VD  +  AWVQ+GA +GE+YY I EKSKTL +PAG+CPTVGVGGH SGGGYG
Sbjct: 128 MFNLRSVDVDVASKTAWVQTGAILGEVYYYIWEKSKTLAYPAGICPTVGVGGHISGGGYG 187

Query: 202 LMLRKFGLAADNVIDAYFIDVNGKLHDRKSMGEDVFWAIRGSGGASYGIVLAWKIKLLPV 261
            M+RK+GL  DN IDA  +DVNGK+ DRK MGED++WAI G GG SYG+VLA+KI L+ V
Sbjct: 188 NMMRKYGLTVDNTIDARMVDVNGKILDRKLMGEDLYWAINGGGGGSYGVVLAYKINLVEV 247

Query: 262 PPIVTVFTIARTLEQNATDIIHRWQYVSSKQDDKLFIRIILTGINTTNSQHGNKRTIEAA 321
           P  VTVF I+RTLEQNATDIIHRWQ V+ K  D+LFIR ++  +N T S   +++T+   
Sbjct: 248 PENVTVFRISRTLEQNATDIIHRWQQVAPKLPDELFIRTVIDVVNGTVS---SQKTVRTT 307

Query: 322 FNSLFLGKIEELVPIMQKTFPELGLTKEDCIEMSWIESVLYFAGFSRGQPLNVLLDRRPL 381
           F ++FLG    L+ I+ + FPELGL + DC E SWI+SVL++     G    +LL R   
Sbjct: 308 FIAMFLGDTTTLLSILNRRFPELGLVRSDCTETSWIQSVLFWTNIQVGSSETLLLQRN-- 367

Query: 382 TPKRFFKAKSDYVNEPIPKAGLEGIWEFFNEEEAEAAVLILSPYGGIMDKISESEIPFPH 441
            P  + K KSDYV EPI + GLE IW+     E E   +  +PYGG M +IS +  PFP+
Sbjct: 368 QPVNYLKRKSDYVREPISRTGLESIWK--KMIELEIPTMAFNPYGGEMGRISSTVTPFPY 427

Query: 442 RAGNLYKIQHLVYWDEEGEDIAKRHINWIRKLYSYMAPFVSKNPRAAYINYRDLDIGTNE 501
           RAGNL+KIQ+   W +  E +  R++   RKLY +M PFVSKNPR ++ NYRD+D+G N 
Sbjct: 428 RAGNLWKIQYGANWRD--ETLTDRYMELTRKLYQFMTPFVSKNPRQSFFNYRDVDLGINS 487

Query: 502 KNGRNTSYNEASVWGVKYFKGNFKRLVSVKTKVDPSNFFKNEQSIPSL 546
            NG+ +SY E   +G KYF GNF+RLV +KT+VD  NFF+NEQSIP L
Sbjct: 488 HNGKISSYVEGKRYGKKYFAGNFERLVKIKTRVDSGNFFRNEQSIPVL 526

BLAST of CSPI01G24740 vs. NCBI nr
Match: gi|449435880|ref|XP_004135722.1| (PREDICTED: cannabidiolic acid synthase-like [Cucumis sativus])

HSP 1 Score: 1108.2 bits (2865), Expect = 0.0e+00
Identity = 541/543 (99.63%), Postives = 542/543 (99.82%), Query Frame = 1

Query: 12  MKSLITSFAHIFIVLTFVWAASAHNHESFLHCLSHHSPNTSSISKIIYTPTNPSYSSVLN 71
           MKSLITSFAHIFIVLTF WAASAHNHESFLHCLSHHSPNTSSISKIIYTPTNPSYSSVLN
Sbjct: 1   MKSLITSFAHIFIVLTFAWAASAHNHESFLHCLSHHSPNTSSISKIIYTPTNPSYSSVLN 60

Query: 72  FSIHNLRFTSPKTPKPQVIVTPFHVSQIQASIICAKNTGFQIRTRSGGHDYEGLSYVSDV 131
           FSIHNLRFTSPKTPKPQVIVTPFHVSQIQASIICAKNTGFQIRTRSGGHDYEGLSYVSDV
Sbjct: 61  FSIHNLRFTSPKTPKPQVIVTPFHVSQIQASIICAKNTGFQIRTRSGGHDYEGLSYVSDV 120

Query: 132 SFVVVDLINLRSISVDAENNVAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHF 191
           SFVVVDLINLRSISVDAENNVAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHF
Sbjct: 121 SFVVVDLINLRSISVDAENNVAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHF 180

Query: 192 SGGGYGLMLRKFGLAADNVIDAYFIDVNGKLHDRKSMGEDVFWAIRGSGGASYGIVLAWK 251
           SGGGYGLMLRKFGLAADNVIDAYFIDVNGKLHDRKSMGEDVFWAIRGSGGASYGIVLAWK
Sbjct: 181 SGGGYGLMLRKFGLAADNVIDAYFIDVNGKLHDRKSMGEDVFWAIRGSGGASYGIVLAWK 240

Query: 252 IKLLPVPPIVTVFTIARTLEQNATDIIHRWQYVSSKQDDKLFIRIILTGINTTNSQHGNK 311
           IKLLPVPPIVTVFTIARTLEQNATDIIHRWQYVSSKQDDKLFIRIILTGINTTNSQHGNK
Sbjct: 241 IKLLPVPPIVTVFTIARTLEQNATDIIHRWQYVSSKQDDKLFIRIILTGINTTNSQHGNK 300

Query: 312 RTIEAAFNSLFLGKIEELVPIMQKTFPELGLTKEDCIEMSWIESVLYFAGFSRGQPLNVL 371
           RTIEAAFNSLFLGKIEELVPIMQKTFPELGLTKEDCIEMSWIESVLYFAGFSRGQPLNVL
Sbjct: 301 RTIEAAFNSLFLGKIEELVPIMQKTFPELGLTKEDCIEMSWIESVLYFAGFSRGQPLNVL 360

Query: 372 LDRRPLTPKRFFKAKSDYVNEPIPKAGLEGIWEFFNEEEAEAAVLILSPYGGIMDKISES 431
           LDRRPLTPKRFFKAKSDYVNEPIPKAGLEGIWEFFNEEEAEAAVLILSPYGGIMDKISES
Sbjct: 361 LDRRPLTPKRFFKAKSDYVNEPIPKAGLEGIWEFFNEEEAEAAVLILSPYGGIMDKISES 420

Query: 432 EIPFPHRAGNLYKIQHLVYWDEEGEDIAKRHINWIRKLYSYMAPFVSKNPRAAYINYRDL 491
           EIPFPHRAGNLYKIQHLVYWDEEGEDIAKRHINWIRKLYSYMAPFVSKNPRAAYINYRDL
Sbjct: 421 EIPFPHRAGNLYKIQHLVYWDEEGEDIAKRHINWIRKLYSYMAPFVSKNPRAAYINYRDL 480

Query: 492 DIGTNEKNGRNTSYNEASVWGVKYFKGNFKRLVSVKTKVDPSNFFKNEQSIPSLKPWWNK 551
           DIGTNEKNGRNTSYNEASVWG+KYFKGNFKRLVSVKTKVDPSNFFKNEQSIPSLKPWWNK
Sbjct: 481 DIGTNEKNGRNTSYNEASVWGIKYFKGNFKRLVSVKTKVDPSNFFKNEQSIPSLKPWWNK 540

Query: 552 RGY 555
           RGY
Sbjct: 541 RGY 543

BLAST of CSPI01G24740 vs. NCBI nr
Match: gi|659068679|ref|XP_008445653.1| (PREDICTED: tetrahydrocannabinolic acid synthase-like [Cucumis melo])

HSP 1 Score: 1082.4 bits (2798), Expect = 0.0e+00
Identity = 528/543 (97.24%), Postives = 534/543 (98.34%), Query Frame = 1

Query: 12  MKSLITSFAHIFIVLTFVWAASAHNHESFLHCLSHHSPNTSSISKIIYTPTNPSYSSVLN 71
           MKSLITSF HIFIVL+F WAASAHNHESFLHCLSHHSPNTSSISKIIYTPTNPSYSSVLN
Sbjct: 1   MKSLITSFLHIFIVLSFAWAASAHNHESFLHCLSHHSPNTSSISKIIYTPTNPSYSSVLN 60

Query: 72  FSIHNLRFTSPKTPKPQVIVTPFHVSQIQASIICAKNTGFQIRTRSGGHDYEGLSYVSDV 131
           FSIHNLRFTSPKTPKP +IVTPF VSQIQASIICAK TGFQIRTRSGGHDYEGLSYVSDV
Sbjct: 61  FSIHNLRFTSPKTPKPHIIVTPFLVSQIQASIICAKKTGFQIRTRSGGHDYEGLSYVSDV 120

Query: 132 SFVVVDLINLRSISVDAENNVAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHF 191
           SFVVVDLINLRSISVDAENNVAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHF
Sbjct: 121 SFVVVDLINLRSISVDAENNVAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHF 180

Query: 192 SGGGYGLMLRKFGLAADNVIDAYFIDVNGKLHDRKSMGEDVFWAIRGSGGASYGIVLAWK 251
           SGGGYGLMLRKFGLAADNVIDAYFIDVNGKLHDRKSMGEDVFWAIRG GGAS+GIVLAWK
Sbjct: 181 SGGGYGLMLRKFGLAADNVIDAYFIDVNGKLHDRKSMGEDVFWAIRGGGGASFGIVLAWK 240

Query: 252 IKLLPVPPIVTVFTIARTLEQNATDIIHRWQYVSSKQDDKLFIRIILTGINTTNSQHGNK 311
           IKLLPVP IVTVFTI RTLEQNATD+IHRWQYVSSKQDDKLFIRIILTGINTTNSQHGNK
Sbjct: 241 IKLLPVPSIVTVFTITRTLEQNATDLIHRWQYVSSKQDDKLFIRIILTGINTTNSQHGNK 300

Query: 312 RTIEAAFNSLFLGKIEELVPIMQKTFPELGLTKEDCIEMSWIESVLYFAGFSRGQPLNVL 371
           RTIEAAFNSLFLGKIEELVPIM+KTFPELGLTKEDCIEMSWIESVLYFAGFSRGQPLNVL
Sbjct: 301 RTIEAAFNSLFLGKIEELVPIMEKTFPELGLTKEDCIEMSWIESVLYFAGFSRGQPLNVL 360

Query: 372 LDRRPLTPKRFFKAKSDYVNEPIPKAGLEGIWEFFNEEEAEAAVLILSPYGGIMDKISES 431
           LDRRPLTPKRFFKAKSDYVNEPIPKAGLEGIWE F+EEEAEAAVLILSPYGGIMDKISES
Sbjct: 361 LDRRPLTPKRFFKAKSDYVNEPIPKAGLEGIWELFDEEEAEAAVLILSPYGGIMDKISES 420

Query: 432 EIPFPHRAGNLYKIQHLVYWDEEGEDIAKRHINWIRKLYSYMAPFVSKNPRAAYINYRDL 491
           EIPFPHRAGNLYKIQHLVYWDEEGEDIAKRHINWIRKLYSYMAPFVSKNPRAAYINYRDL
Sbjct: 421 EIPFPHRAGNLYKIQHLVYWDEEGEDIAKRHINWIRKLYSYMAPFVSKNPRAAYINYRDL 480

Query: 492 DIGTNEKNGRNTSYNEASVWGVKYFKGNFKRLVSVKTKVDPSNFFKNEQSIPSLKPWWNK 551
           DIGTNEKNGRNTSYNEASVWGVKYFKGNFKRLVSVKTKVDPSNFFKNEQSIPSLKPWWNK
Sbjct: 481 DIGTNEKNGRNTSYNEASVWGVKYFKGNFKRLVSVKTKVDPSNFFKNEQSIPSLKPWWNK 540

Query: 552 RGY 555
           RGY
Sbjct: 541 RGY 543

BLAST of CSPI01G24740 vs. NCBI nr
Match: gi|565380209|ref|XP_006356501.1| (PREDICTED: cannabidiolic acid synthase-like [Solanum tuberosum])

HSP 1 Score: 743.4 bits (1918), Expect = 2.8e-211
Identity = 361/542 (66.61%), Postives = 440/542 (81.18%), Query Frame = 1

Query: 12  MKSLITSFAHIFIVLTFV-WAASAHNHESFLHCLSHHSPNTSSISKIIYTPTNPSYSSVL 71
           MK    SF  + +VL+   W+A A NHE F+ CLSH +  +S+I    YTP N S+ S+L
Sbjct: 1   MKISWFSFIFVLVVLSSASWSALADNHEEFIQCLSHSNQTSSNI----YTPNNSSFPSIL 60

Query: 72  NFSIHNLRFTSPKTPKPQVIVTPFHVSQIQASIICAKNTGFQIRTRSGGHDYEGLSYVSD 131
            FSI NLRF + +TPKP VIVTP   S++Q  I+C+K TG  +R R  GHDYEGLSYVS+
Sbjct: 61  QFSIQNLRFNTTETPKPLVIVTPVSESEVQRVILCSKKTGMHVRVRGAGHDYEGLSYVSE 120

Query: 132 VSFVVVDLINLRSISVDAENNVAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGH 191
           V FV+VDLINLR+I+VD  +  AWV++G+TIGELYYRIAEKSKTLGFPAGVCPTVGVGGH
Sbjct: 121 VPFVIVDLINLRTINVDVNDKSAWVEAGSTIGELYYRIAEKSKTLGFPAGVCPTVGVGGH 180

Query: 192 FSGGGYGLMLRKFGLAADNVIDAYFIDVNGKLHDRKSMGEDVFWAIRGSGGASYGIVLAW 251
           FSGGGYG+MLRK+GLAADN++DA  ID NG++ DR SMGED+FWAIRG GG ++G+VLAW
Sbjct: 181 FSGGGYGVMLRKYGLAADNIVDARLIDANGRILDRVSMGEDLFWAIRGGGGNTFGLVLAW 240

Query: 252 KIKLLPVPPIVTVFTIARTLEQNATDIIHRWQYVSSKQDDKLFIRIILTGINTTNSQHG- 311
           KIKL+ VP IVTVFT+ +TLEQNAT ++HRWQYV+ +  + LFIRI+++ +N++N   G 
Sbjct: 241 KIKLVDVPEIVTVFTLDKTLEQNATKLVHRWQYVAPRFHEDLFIRILVSKLNSSNQGDGN 300

Query: 312 NKRTIEAAFNSLFLGKIEELVPIMQKTFPELGLTKEDCIEMSWIESVLYFAGFSRGQPLN 371
           N++TI A+FNS+FLG I+ L+PIM++ FPELGL +EDCIEMSWIES++YFAGF    PL+
Sbjct: 301 NQQTIVASFNSIFLGGIDRLLPIMKENFPELGLRREDCIEMSWIESIMYFAGFPTDGPLD 360

Query: 372 VLLDRRPLTPKRFFKAKSDYVNEPIPKAGLEGIWEFFNEEEAEAAVLILSPYGGIMDKIS 431
           VLL R  L+  R+FKAKSDYV +PIP+ GLEGIW FF E+EA+++ +ILSPYGG MD+IS
Sbjct: 361 VLLSRVQLS-TRYFKAKSDYVYQPIPEGGLEGIWRFFFEDEAQSSQVILSPYGGRMDEIS 420

Query: 432 ESEIPFPHRAGNLYKIQHLVYWDEEGEDIAKRHINWIRKLYSYMAPFVSKNPRAAYINYR 491
            S IPFPHRAGNLYKIQHLVYWDEEGE++A+RHI+WIR+LYSYMAPFVSK PRAAYINYR
Sbjct: 421 PSAIPFPHRAGNLYKIQHLVYWDEEGEEVAERHISWIRRLYSYMAPFVSKLPRAAYINYR 480

Query: 492 DLDIGTNEKNGRNTSYNEASVWGVKYFKGNFKRLVSVKTKVDPSNFFKNEQSIPSLKPWW 551
           DLDIG N   G  TSY +A VWG+KYFK NF RLV VKTKVDPSNFF+NEQSIPSL  W 
Sbjct: 481 DLDIGVNNIKG-YTSYVQAKVWGIKYFKNNFDRLVHVKTKVDPSNFFRNEQSIPSLTQWK 536

BLAST of CSPI01G24740 vs. NCBI nr
Match: gi|460393282|ref|XP_004242239.1| (PREDICTED: cannabidiolic acid synthase-like [Solanum lycopersicum])

HSP 1 Score: 742.7 bits (1916), Expect = 4.8e-211
Identity = 358/540 (66.30%), Postives = 438/540 (81.11%), Query Frame = 1

Query: 16  ITSFAHIFIVLTFV---WAASAHNHESFLHCLSHHSPNTSSISKIIYTPTNPSYSSVLNF 75
           I+ F+ IF++L      W+  A NHE F+ CLSH++  +S+I    YTP N S+ S+L F
Sbjct: 3   ISWFSFIFVLLVLSSASWSTLADNHEEFIQCLSHNNQTSSNI----YTPNNSSFQSILQF 62

Query: 76  SIHNLRFTSPKTPKPQVIVTPFHVSQIQASIICAKNTGFQIRTRSGGHDYEGLSYVSDVS 135
           SI NLRF + +TPKP VIVTP   S++Q  I+CAK TG  +R R  GHDYEGLSYVS+V 
Sbjct: 63  SIQNLRFNTSETPKPLVIVTPVSESEVQRVILCAKKTGMHVRVRGAGHDYEGLSYVSEVP 122

Query: 136 FVVVDLINLRSISVDAENNVAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHFS 195
           FV+VDLINLR+I+VD  +  AWV++G+TIGELYYRIAEKSKTLGFPAGVCPTVGVGGHFS
Sbjct: 123 FVIVDLINLRTINVDVNDKSAWVEAGSTIGELYYRIAEKSKTLGFPAGVCPTVGVGGHFS 182

Query: 196 GGGYGLMLRKFGLAADNVIDAYFIDVNGKLHDRKSMGEDVFWAIRGSGGASYGIVLAWKI 255
           GGGYG+MLRK+GLAADN++DA  ID NG++ DR SMGED+FWAIRG GG S+G+VLAWK+
Sbjct: 183 GGGYGVMLRKYGLAADNIVDARLIDANGRILDRASMGEDLFWAIRGGGGNSFGLVLAWKV 242

Query: 256 KLLPVPPIVTVFTIARTLEQNATDIIHRWQYVSSKQDDKLFIRIILTGINTTN-SQHGNK 315
           KL+ VP IVTVFT+ +TLEQNAT ++H+WQYV+ +    LFIRI+++ +N++N     N+
Sbjct: 243 KLVDVPEIVTVFTLDKTLEQNATKLVHKWQYVAPRFHQDLFIRILVSRLNSSNQGDDNNQ 302

Query: 316 RTIEAAFNSLFLGKIEELVPIMQKTFPELGLTKEDCIEMSWIESVLYFAGFSRGQPLNVL 375
           +TI A+FNS+FLG I+ L+PIMQ+ FPELGL + DCIEMSWIES+LYFAGF    PL+VL
Sbjct: 303 QTIVASFNSIFLGGIDRLLPIMQENFPELGLRRADCIEMSWIESILYFAGFPTDGPLDVL 362

Query: 376 LDRRPLTPKRFFKAKSDYVNEPIPKAGLEGIWEFFNEEEAEAAVLILSPYGGIMDKISES 435
           L R  L+  R+FKAKSDYV +PIP+ GL+GIW FF E+EA+++ +ILSPYGG MD+IS S
Sbjct: 363 LSRVQLS-TRYFKAKSDYVYQPIPEGGLDGIWRFFFEDEAQSSQVILSPYGGRMDEISPS 422

Query: 436 EIPFPHRAGNLYKIQHLVYWDEEGEDIAKRHINWIRKLYSYMAPFVSKNPRAAYINYRDL 495
            IPFPHRAGNLYKIQHLVYWDEEGE++A+RHI+WIR+LYSYMAPFVSK+PRAAYINYRDL
Sbjct: 423 AIPFPHRAGNLYKIQHLVYWDEEGEEVAERHISWIRRLYSYMAPFVSKSPRAAYINYRDL 482

Query: 496 DIGTNEKNGRNTSYNEASVWGVKYFKGNFKRLVSVKTKVDPSNFFKNEQSIPSLKPWWNK 552
           DIG N   G  TSY +A VWG+KYFK NF RLV VKTKVDPSNFF+NEQSIPSL  W NK
Sbjct: 483 DIGVNNIKG-YTSYVQAKVWGIKYFKNNFDRLVHVKTKVDPSNFFRNEQSIPSLTQWKNK 536

BLAST of CSPI01G24740 vs. NCBI nr
Match: gi|970034929|ref|XP_015078794.1| (PREDICTED: cannabidiolic acid synthase-like [Solanum pennellii])

HSP 1 Score: 740.7 bits (1911), Expect = 1.8e-210
Identity = 356/540 (65.93%), Postives = 436/540 (80.74%), Query Frame = 1

Query: 16  ITSFAHIFIVLTFV---WAASAHNHESFLHCLSHHSPNTSSISKIIYTPTNPSYSSVLNF 75
           I+ F+ IF++L      W+  A NHE F+ CLSH    ++ IS  +YTP N S+ S+L F
Sbjct: 3   ISWFSFIFVLLVLSSASWSTLADNHEEFIQCLSH----SNQISSNVYTPNNSSFQSILQF 62

Query: 76  SIHNLRFTSPKTPKPQVIVTPFHVSQIQASIICAKNTGFQIRTRSGGHDYEGLSYVSDVS 135
           SI NLRF + +TPKP VIVTP   +++Q  I+CAK TG  +R R  GHDYEGLSYVS+V 
Sbjct: 63  SIQNLRFNTSETPKPLVIVTPVSETEVQRVILCAKKTGMHVRVRGAGHDYEGLSYVSEVP 122

Query: 136 FVVVDLINLRSISVDAENNVAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHFS 195
           FV+VDLINLR I+VD  +  AWV++G+TIGELYYRIAEKSKTLGFPAGVCPTVGVGGHFS
Sbjct: 123 FVIVDLINLRKINVDVNDKSAWVEAGSTIGELYYRIAEKSKTLGFPAGVCPTVGVGGHFS 182

Query: 196 GGGYGLMLRKFGLAADNVIDAYFIDVNGKLHDRKSMGEDVFWAIRGSGGASYGIVLAWKI 255
           GGGYG+MLRK+GLAADN++DA  +D NG++ DR SMGED+FWAIRG GG S+G+VLAWK+
Sbjct: 183 GGGYGVMLRKYGLAADNIVDARLLDANGRILDRASMGEDLFWAIRGGGGNSFGLVLAWKV 242

Query: 256 KLLPVPPIVTVFTIARTLEQNATDIIHRWQYVSSKQDDKLFIRIILTGINTTN-SQHGNK 315
           KL+ VP IVTVFT+ +TLEQNAT ++H+WQYV+ +  + LFIRI+++ +N++N     N+
Sbjct: 243 KLVDVPEIVTVFTLDKTLEQNATKLVHKWQYVAPRFHEDLFIRILVSRLNSSNQGDDNNQ 302

Query: 316 RTIEAAFNSLFLGKIEELVPIMQKTFPELGLTKEDCIEMSWIESVLYFAGFSRGQPLNVL 375
           +TI A+FNS+FLG I+ L+PIM + FPELGL +EDCIEMSWIES+LYFAGF    PL+VL
Sbjct: 303 QTIVASFNSIFLGGIDRLLPIMHENFPELGLRREDCIEMSWIESILYFAGFPTDGPLDVL 362

Query: 376 LDRRPLTPKRFFKAKSDYVNEPIPKAGLEGIWEFFNEEEAEAAVLILSPYGGIMDKISES 435
           L R  L+  R+FKAKSDYV +PIP+ GL GIW FF E+EA+++ +ILSPYGG MD+IS S
Sbjct: 363 LSRVQLS-TRYFKAKSDYVYQPIPEGGLNGIWRFFFEDEAQSSQVILSPYGGRMDEISPS 422

Query: 436 EIPFPHRAGNLYKIQHLVYWDEEGEDIAKRHINWIRKLYSYMAPFVSKNPRAAYINYRDL 495
            IPFPHRAGNLYKIQHLVYWDEEGE++A+RHI+WIR+LYSYMAPFVSK+PRAAYINYRDL
Sbjct: 423 AIPFPHRAGNLYKIQHLVYWDEEGEEVAERHISWIRRLYSYMAPFVSKSPRAAYINYRDL 482

Query: 496 DIGTNEKNGRNTSYNEASVWGVKYFKGNFKRLVSVKTKVDPSNFFKNEQSIPSLKPWWNK 552
           DIG N   G  TSY +A VWG+KYFK NF RLV VKTKVDPSNFF+NEQSIPSL  W NK
Sbjct: 483 DIGVNNIKG-YTSYVQAKVWGIKYFKNNFDRLVHVKTKVDPSNFFRNEQSIPSLTQWKNK 536