ClCG03G002520 (gene) Watermelon (Charleston Gray)

BLAST of ClCG03G002520 vs. Swiss-Prot
Match: THCAI_CANSA (Inactive tetrahydrocannabinolic acid synthase OS=Cannabis sativa PE=3 SV=1)

HSP 1 Score: 214.2 bits (544), Expect = 5.7e-54
Identity = 105/237 (44.30%), Postives = 148/237 (62.45%), Query Frame = 1

Query: 167 TIEASFFSLFLGNASELLALMEKIFPELGLKKEDCLEMSWVEAMAFSASGFVSAQSL--- 226
           T+   F S+FLG    L+ LM K FPELG+KK DC E+SW++   F  SG V+  +    
Sbjct: 308 TVHGYFSSIFLGGVDSLVDLMNKSFPELGIKKTDCKELSWIDTTIFY-SGVVNYNTANFK 367

Query: 227 -ELLLDRIPPKIGRNKIKSDYATQPISKIALEGMWERFKIEELETVQLILIPFGGKMNEI 286
            E+LLDR   K     IK DY  + I + A+  + E+   EE+     +L P+GG M+EI
Sbjct: 368 KEILLDRSAGKKTAFSIKLDYVKKLIPETAMVKILEKLYEEEVGVGMYVLYPYGGIMDEI 427

Query: 287 SEAESPSPHRAGYPIHITYYVTWQQPDADSRHLEWTRELHDYMTPFVSKSPRAAYINHRD 346
           SE+  P PHRAG    + Y  TW++ + + +H+ W R ++++ TP+VS++PR AY+N+RD
Sbjct: 428 SESAIPFPHRAGIMYELWYTATWEKQEDNEKHINWVRSVYNFTTPYVSQNPRLAYLNYRD 487

Query: 347 FDIGTNNEDGIPTSYEEASVWGQPYFGNNFERLMEVKRKVDPFNFFRHEQSIPPAPP 400
            D+G  N +  P +Y +A +WG+ YFG NF RL++VK K DP NFFR+EQSIPP PP
Sbjct: 488 LDLGKTNPES-PNNYTQARIWGEKYFGKNFNRLVKVKTKADPNNFFRNEQSIPPLPP 542

HSP 2 Score: 201.1 bits (510), Expect = 5.0e-50
Identity = 104/238 (43.70%), Postives = 150/238 (63.03%), Query Frame = 1

Query: 592 TMEASFFSLFLGNASELLAHMEKIFPELGLKKEDCLEMSWVEAMAFSASGFVSAQSL--- 651
           T+   F S+FLG    L+  M K FPELG+KK DC E+SW++   F  SG V+  +    
Sbjct: 308 TVHGYFSSIFLGGVDSLVDLMNKSFPELGIKKTDCKELSWIDTTIFY-SGVVNYNTANFK 367

Query: 652 -ELLLNRTPPKIGRYKTKSDYATQPISKTA----LEGMFKFEELETVRLVLIPFRGKMNE 711
            E+LL+R+  K   +  K DY  + I +TA    LE +++ EE+     VL P+ G M+E
Sbjct: 368 KEILLDRSAGKKTAFSIKLDYVKKLIPETAMVKILEKLYE-EEVGVGMYVLYPYGGIMDE 427

Query: 712 ISEVEIPSPHRAGYPIHIAYYVTWQRPDADSRHLEWMQELHDYMTPFVSKSPRAAYINCR 771
           ISE  IP PHRAG    + Y  TW++ + + +H+ W++ ++++ TP+VS++PR AY+N R
Sbjct: 428 ISESAIPFPHRAGIMYELWYTATWEKQEDNEKHINWVRSVYNFTTPYVSQNPRLAYLNYR 487

Query: 772 DLDIGTNNKDGISMSYEEASVWGQPYFGNNFERLMEVKRKVDPFNFFRHEQSIPPAPP 822
           DLD+G  N +  + +Y +A +WG+ YFG NF RL++VK K DP NFFR+EQSIPP PP
Sbjct: 488 DLDLGKTNPESPN-NYTQARIWGEKYFGKNFNRLVKVKTKADPNNFFRNEQSIPPLPP 542

HSP 3 Score: 126.7 bits (317), Expect = 1.2e-27
Identity = 59/125 (47.20%), Postives = 83/125 (66.40%), Query Frame = 1

Query: 457 EDFVHCVYLHSNNNNDSNSTLIHTPSSSSYSHVLNFSIRNRRFSETETPKPVAIITPSHA 516
           E+F+ C +     NN +N   I+T     Y  VLN +I+N RF+   TPKP+ I+TPS+ 
Sbjct: 32  ENFLKC-FSEYIPNNPANPKFIYTQHDQLYMSVLNSTIQNLRFTSDTTPKPLVIVTPSNV 91

Query: 517 SQVQATVICCKSHGLQIRTRSGGHDLEGRSYVANVPFVLVDLINFNLITIDTEDKSAWVQ 576
           S +QA+++C K  GLQIRTRSGGHD EG SY++ VPF +VDL N + + +D   ++AWV+
Sbjct: 92  SHIQASILCSKKVGLQIRTRSGGHDAEGLSYISQVPFAIVDLRNMHTVKVDIHSQTAWVE 151

Query: 577 SGATV 582
           +GAT+
Sbjct: 152 AGATL 155

HSP 4 Score: 122.5 bits (306), Expect = 2.3e-26
Identity = 62/153 (40.52%), Postives = 94/153 (61.44%), Query Frame = 1

Query: 6   SCYGYCFISLLLLFSLA-TLGCGDDN-LEDFVHCVYLHSNNNNDSNSTLIHIPSSSSYSH 65
           S + + F+  ++ F L+  +     N  E+F+ C +     NN +N   I+      Y  
Sbjct: 4   SAFSFWFVCKIIFFFLSFNIQISIANPQENFLKC-FSEYIPNNPANPKFIYTQHDQLYMS 63

Query: 66  VLNFSIRNRRFSEAETPKPVAIITPSHAFQVQATVICCKSHGLQIRTRSGGHDFEGRSYV 125
           VLN +I+N RF+   TPKP+ I+TPS+   +QA+++C K  GLQIRTRSGGHD EG SY+
Sbjct: 64  VLNSTIQNLRFTSDTTPKPLVIVTPSNVSHIQASILCSKKVGLQIRTRSGGHDAEGLSYI 123

Query: 126 ANVPFVLVDLINFNSITIDTEDKSAWVQSGATV 157
           + VPF +VDL N +++ +D   ++AWV++GAT+
Sbjct: 124 SQVPFAIVDLRNMHTVKVDIHSQTAWVEAGATL 155

BLAST of ClCG03G002520 vs. Swiss-Prot
Match: CBDAS_CANSA (Cannabidiolic acid synthase OS=Cannabis sativa GN=CBDAS PE=1 SV=1)

HSP 1 Score: 214.2 bits (544), Expect = 5.7e-54
Identity = 108/243 (44.44%), Postives = 153/243 (62.96%), Query Frame = 1

Query: 161 SNGSGRT-IEASFFSLFLGNASELLALMEKIFPELGLKKEDCLEMSWVEAMAFSASGFVS 220
           + G  +T I   F S+FLG    L+ LM K FPELG+KK DC ++SW++ + F  SG V+
Sbjct: 300 NQGKNKTAIHTYFSSVFLGGVDSLVDLMNKSFPELGIKKTDCRQLSWIDTIIFY-SGVVN 359

Query: 221 AQS----LELLLDRIPPKIGRNKIKSDYATQPISKIALEGMWERFKIEELETVQLILIPF 280
             +     E+LLDR   + G  KIK DY  +PI +     + E+   E++      L P+
Sbjct: 360 YDTDNFNKEILLDRSAGQNGAFKIKLDYVKKPIPESVFVQILEKLYEEDIGAGMYALYPY 419

Query: 281 GGKMNEISEAESPSPHRAGYPIHITYYVTWQQPDADSRHLEWTRELHDYMTPFVSKSPRA 340
           GG M+EISE+  P PHRAG    + Y  +W++ + + +HL W R ++++MTP+VSK+PR 
Sbjct: 420 GGIMDEISESAIPFPHRAGILYELWYICSWEKQEDNEKHLNWIRNIYNFMTPYVSKNPRL 479

Query: 341 AYINHRDFDIGTNNEDGIPTSYEEASVWGQPYFGNNFERLMEVKRKVDPFNFFRHEQSIP 399
           AY+N+RD DIG N+    P +Y +A +WG+ YFG NF+RL++VK  VDP NFFR+EQSIP
Sbjct: 480 AYLNYRDLDIGINDPKN-PNNYTQARIWGEKYFGKNFDRLVKVKTLVDPNNFFRNEQSIP 539

HSP 2 Score: 201.1 bits (510), Expect = 5.0e-50
Identity = 104/247 (42.11%), Postives = 158/247 (63.97%), Query Frame = 1

Query: 583 LNSSNGGGRTMEASFFS-LFLGNASELLAHMEKIFPELGLKKEDCLEMSWVEAMAFSASG 642
           +  + G  +T   ++FS +FLG    L+  M K FPELG+KK DC ++SW++ + F  SG
Sbjct: 297 ITDNQGKNKTAIHTYFSSVFLGGVDSLVDLMNKSFPELGIKKTDCRQLSWIDTIIFY-SG 356

Query: 643 FVSAQS----LELLLNRTPPKIGRYKTKSDYATQPISKTA----LEGMFKFEELETVRLV 702
            V+  +     E+LL+R+  + G +K K DY  +PI ++     LE +++ E++      
Sbjct: 357 VVNYDTDNFNKEILLDRSAGQNGAFKIKLDYVKKPIPESVFVQILEKLYE-EDIGAGMYA 416

Query: 703 LIPFRGKMNEISEVEIPSPHRAGYPIHIAYYVTWQRPDADSRHLEWMQELHDYMTPFVSK 762
           L P+ G M+EISE  IP PHRAG    + Y  +W++ + + +HL W++ ++++MTP+VSK
Sbjct: 417 LYPYGGIMDEISESAIPFPHRAGILYELWYICSWEKQEDNEKHLNWIRNIYNFMTPYVSK 476

Query: 763 SPRAAYINCRDLDIGTNNKDGISMSYEEASVWGQPYFGNNFERLMEVKRKVDPFNFFRHE 821
           +PR AY+N RDLDIG N+    + +Y +A +WG+ YFG NF+RL++VK  VDP NFFR+E
Sbjct: 477 NPRLAYLNYRDLDIGINDPKNPN-NYTQARIWGEKYFGKNFDRLVKVKTLVDPNNFFRNE 536

HSP 3 Score: 132.1 bits (331), Expect = 2.8e-29
Identity = 63/125 (50.40%), Postives = 84/125 (67.20%), Query Frame = 1

Query: 457 EDFVHCVYLHSNNNNDSNSTLIHTPSSSSYSHVLNFSIRNRRFSETETPKPVAIITPSHA 516
           E+F+ C +     NN +N  L++T ++  Y  VLN +I N RF+   TPKP+ I+TPSH 
Sbjct: 32  ENFLKC-FSQYIPNNATNLKLVYTQNNPLYMSVLNSTIHNLRFTSDTTPKPLVIVTPSHV 91

Query: 517 SQVQATVICCKSHGLQIRTRSGGHDLEGRSYVANVPFVLVDLINFNLITIDTEDKSAWVQ 576
           S +Q T++C K  GLQIRTRSGGHD EG SY++ VPFV+VDL N   I ID   ++AWV+
Sbjct: 92  SHIQGTILCSKKVGLQIRTRSGGHDSEGMSYISQVPFVIVDLRNMRSIKIDVHSQTAWVE 151

Query: 577 SGATV 582
           +GAT+
Sbjct: 152 AGATL 155

HSP 4 Score: 129.4 bits (324), Expect = 1.8e-28
Identity = 62/125 (49.60%), Postives = 83/125 (66.40%), Query Frame = 1

Query: 32  EDFVHCVYLHSNNNNDSNSTLIHIPSSSSYSHVLNFSIRNRRFSEAETPKPVAIITPSHA 91
           E+F+ C +     NN +N  L++  ++  Y  VLN +I N RF+   TPKP+ I+TPSH 
Sbjct: 32  ENFLKC-FSQYIPNNATNLKLVYTQNNPLYMSVLNSTIHNLRFTSDTTPKPLVIVTPSHV 91

Query: 92  FQVQATVICCKSHGLQIRTRSGGHDFEGRSYVANVPFVLVDLINFNSITIDTEDKSAWVQ 151
             +Q T++C K  GLQIRTRSGGHD EG SY++ VPFV+VDL N  SI ID   ++AWV+
Sbjct: 92  SHIQGTILCSKKVGLQIRTRSGGHDSEGMSYISQVPFVIVDLRNMRSIKIDVHSQTAWVE 151

Query: 152 SGATV 157
           +GAT+
Sbjct: 152 AGATL 155

BLAST of ClCG03G002520 vs. Swiss-Prot
Match: THCAS_CANSA (Tetrahydrocannabinolic acid synthase OS=Cannabis sativa PE=1 SV=1)

HSP 1 Score: 212.6 bits (540), Expect = 1.7e-53
Identity = 106/251 (42.23%), Postives = 153/251 (60.96%), Query Frame = 1

Query: 156 VASNSSNGSGR---TIEASFFSLFLGNASELLALMEKIFPELGLKKEDCLEMSWVEAMAF 215
           +  N ++  G+   T+   F S+F G    L+ LM K FPELG+KK DC E SW++   F
Sbjct: 294 ITKNITDNHGKNKTTVHGYFSSIFHGGVDSLVDLMNKSFPELGIKKTDCKEFSWIDTTIF 353

Query: 216 SASGFVSAQSL----ELLLDRIPPKIGRNKIKSDYATQPISKIALEGMWERFKIEELETV 275
             SG V+  +     E+LLDR   K     IK DY  +PI + A+  + E+   E++   
Sbjct: 354 Y-SGVVNFNTANFKKEILLDRSAGKKTAFSIKLDYVKKPIPETAMVKILEKLYEEDVGAG 413

Query: 276 QLILIPFGGKMNEISEAESPSPHRAGYPIHITYYVTWQQPDADSRHLEWTRELHDYMTPF 335
             +L P+GG M EISE+  P PHRAG    + Y  +W++ + + +H+ W R ++++ TP+
Sbjct: 414 MYVLYPYGGIMEEISESAIPFPHRAGIMYELWYTASWEKQEDNEKHINWVRSVYNFTTPY 473

Query: 336 VSKSPRAAYINHRDFDIGTNNEDGIPTSYEEASVWGQPYFGNNFERLMEVKRKVDPFNFF 395
           VS++PR AY+N+RD D+G  N    P +Y +A +WG+ YFG NF RL++VK KVDP NFF
Sbjct: 474 VSQNPRLAYLNYRDLDLGKTNH-ASPNNYTQARIWGEKYFGKNFNRLVKVKTKVDPNNFF 533

Query: 396 RHEQSIPPAPP 400
           R+EQSIPP PP
Sbjct: 534 RNEQSIPPLPP 542

HSP 2 Score: 198.4 bits (503), Expect = 3.2e-49
Identity = 103/238 (43.28%), Postives = 148/238 (62.18%), Query Frame = 1

Query: 592 TMEASFFSLFLGNASELLAHMEKIFPELGLKKEDCLEMSWVEAMAFSASGFVSAQSL--- 651
           T+   F S+F G    L+  M K FPELG+KK DC E SW++   F  SG V+  +    
Sbjct: 308 TVHGYFSSIFHGGVDSLVDLMNKSFPELGIKKTDCKEFSWIDTTIFY-SGVVNFNTANFK 367

Query: 652 -ELLLNRTPPKIGRYKTKSDYATQPISKTA----LEGMFKFEELETVRLVLIPFRGKMNE 711
            E+LL+R+  K   +  K DY  +PI +TA    LE +++ E++     VL P+ G M E
Sbjct: 368 KEILLDRSAGKKTAFSIKLDYVKKPIPETAMVKILEKLYE-EDVGAGMYVLYPYGGIMEE 427

Query: 712 ISEVEIPSPHRAGYPIHIAYYVTWQRPDADSRHLEWMQELHDYMTPFVSKSPRAAYINCR 771
           ISE  IP PHRAG    + Y  +W++ + + +H+ W++ ++++ TP+VS++PR AY+N R
Sbjct: 428 ISESAIPFPHRAGIMYELWYTASWEKQEDNEKHINWVRSVYNFTTPYVSQNPRLAYLNYR 487

Query: 772 DLDIGTNNKDGISMSYEEASVWGQPYFGNNFERLMEVKRKVDPFNFFRHEQSIPPAPP 822
           DLD+G  N    + +Y +A +WG+ YFG NF RL++VK KVDP NFFR+EQSIPP PP
Sbjct: 488 DLDLGKTNHASPN-NYTQARIWGEKYFGKNFNRLVKVKTKVDPNNFFRNEQSIPPLPP 542

HSP 3 Score: 130.6 bits (327), Expect = 8.3e-29
Identity = 63/125 (50.40%), Postives = 84/125 (67.20%), Query Frame = 1

Query: 457 EDFVHCVYLHSNNNNDSNSTLIHTPSSSSYSHVLNFSIRNRRFSETETPKPVAIITPSHA 516
           E+F+ C   H  NN  +N  L++T     Y  +LN +I+N RF    TPKP+ I+TPS+ 
Sbjct: 32  ENFLKCFSKHIPNNV-ANPKLVYTQHDQLYMSILNSTIQNLRFISDTTPKPLVIVTPSNN 91

Query: 517 SQVQATVICCKSHGLQIRTRSGGHDLEGRSYVANVPFVLVDLINFNLITIDTEDKSAWVQ 576
           S +QAT++C K  GLQIRTRSGGHD EG SY++ VPFV+VDL N + I ID   ++AWV+
Sbjct: 92  SHIQATILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVVVDLRNMHSIKIDVHSQTAWVE 151

Query: 577 SGATV 582
           +GAT+
Sbjct: 152 AGATL 155

HSP 4 Score: 127.9 bits (320), Expect = 5.4e-28
Identity = 66/154 (42.86%), Postives = 95/154 (61.69%), Query Frame = 1

Query: 6   SCYGYCFISLLLLFSLA---TLGCGDDNLEDFVHCVYLHSNNNNDSNSTLIHIPSSSSYS 65
           S + + F+  ++ F L+    +   +   E+F+ C   H  NN  +N  L++      Y 
Sbjct: 4   SAFSFWFVCKIIFFFLSFHIQISIANPR-ENFLKCFSKHIPNNV-ANPKLVYTQHDQLYM 63

Query: 66  HVLNFSIRNRRFSEAETPKPVAIITPSHAFQVQATVICCKSHGLQIRTRSGGHDFEGRSY 125
            +LN +I+N RF    TPKP+ I+TPS+   +QAT++C K  GLQIRTRSGGHD EG SY
Sbjct: 64  SILNSTIQNLRFISDTTPKPLVIVTPSNNSHIQATILCSKKVGLQIRTRSGGHDAEGMSY 123

Query: 126 VANVPFVLVDLINFNSITIDTEDKSAWVQSGATV 157
           ++ VPFV+VDL N +SI ID   ++AWV++GAT+
Sbjct: 124 ISQVPFVVVDLRNMHSIKIDVHSQTAWVEAGATL 155

BLAST of ClCG03G002520 vs. Swiss-Prot
Match: CASL2_CANSA (Cannabidiolic acid synthase-like 2 OS=Cannabis sativa GN=CBDAS3 PE=2 SV=1)

HSP 1 Score: 211.8 bits (538), Expect = 2.8e-53
Identity = 105/252 (41.67%), Postives = 156/252 (61.90%), Query Frame = 1

Query: 156 VASNSSNGSGR---TIEASFFSLFLGNASELLALMEKIFPELGLKKEDCLEMSWVEAMAF 215
           +  N ++  G+   TI + F S+F G    L+ LM K FPELG+KK DC ++SW++ + F
Sbjct: 294 ITRNITDNQGKNKTTIHSYFSSIFHGGVDSLVDLMNKSFPELGIKKTDCKQLSWIDTIIF 353

Query: 216 SASGFVSAQSL----ELLLDRIPPKIGRNKIKSDYATQPISKIALEGMWERFKIEELETV 275
             SG V+  +     E+LLDR   +     IK DY  +PI + A+  + E+   E++   
Sbjct: 354 Y-SGVVNYNTTNFKKEILLDRSGGRKAAFSIKLDYVKKPIPETAMVTILEKLYEEDVGVG 413

Query: 276 QLILIPFGGKMNEISEAESPSPHRAGYPIHITYYVTWQQPDADSRHLEWTRELHDYMTPF 335
             +  P+GG M+EISE+  P PHRAG    I Y  +W++ + + +H+ W R ++++ TP+
Sbjct: 414 MFVFYPYGGIMDEISESAIPFPHRAGITYEIWYIASWEKQEDNEKHINWIRNVYNFTTPY 473

Query: 336 VSKSPRAAYINHRDFDIGTNNEDGIPTSYEEASVWGQPYFGNNFERLMEVKRKVDPFNFF 395
           VS++PR AY+N+RD D+G  N +  P +Y +A +WG+ YFG NF RL++VK KVDP NFF
Sbjct: 474 VSQNPRMAYLNYRDLDLGKTNFES-PNNYTQARIWGEKYFGKNFNRLVKVKTKVDPDNFF 533

Query: 396 RHEQSIPPAPPR 401
           R+EQSIPP P R
Sbjct: 534 RNEQSIPPLPLR 543

HSP 2 Score: 197.2 bits (500), Expect = 7.2e-49
Identity = 100/237 (42.19%), Postives = 151/237 (63.71%), Query Frame = 1

Query: 592 TMEASFFSLFLGNASELLAHMEKIFPELGLKKEDCLEMSWVEAMAFSASGFVSAQSL--- 651
           T+ + F S+F G    L+  M K FPELG+KK DC ++SW++ + F  SG V+  +    
Sbjct: 308 TIHSYFSSIFHGGVDSLVDLMNKSFPELGIKKTDCKQLSWIDTIIFY-SGVVNYNTTNFK 367

Query: 652 -ELLLNRTPPKIGRYKTKSDYATQPISKTA----LEGMFKFEELETVRLVLIPFRGKMNE 711
            E+LL+R+  +   +  K DY  +PI +TA    LE +++ E++     V  P+ G M+E
Sbjct: 368 KEILLDRSGGRKAAFSIKLDYVKKPIPETAMVTILEKLYE-EDVGVGMFVFYPYGGIMDE 427

Query: 712 ISEVEIPSPHRAGYPIHIAYYVTWQRPDADSRHLEWMQELHDYMTPFVSKSPRAAYINCR 771
           ISE  IP PHRAG    I Y  +W++ + + +H+ W++ ++++ TP+VS++PR AY+N R
Sbjct: 428 ISESAIPFPHRAGITYEIWYIASWEKQEDNEKHINWIRNVYNFTTPYVSQNPRMAYLNYR 487

Query: 772 DLDIGTNNKDGISMSYEEASVWGQPYFGNNFERLMEVKRKVDPFNFFRHEQSIPPAP 821
           DLD+G  N +  + +Y +A +WG+ YFG NF RL++VK KVDP NFFR+EQSIPP P
Sbjct: 488 DLDLGKTNFESPN-NYTQARIWGEKYFGKNFNRLVKVKTKVDPDNFFRNEQSIPPLP 541

HSP 3 Score: 124.8 bits (312), Expect = 4.5e-27
Identity = 58/125 (46.40%), Postives = 84/125 (67.20%), Query Frame = 1

Query: 457 EDFVHCVYLHSNNNNDSNSTLIHTPSSSSYSHVLNFSIRNRRFSETETPKPVAIITPSHA 516
           E+F+ C+  +   N  +N+ L++T     Y  +LN +++N RF+   TPKP+ I TP + 
Sbjct: 32  ENFLKCLSQYIPTNV-TNAKLVYTQHDQFYMSILNSTVQNLRFTSDTTPKPLVITTPLNV 91

Query: 517 SQVQATVICCKSHGLQIRTRSGGHDLEGRSYVANVPFVLVDLINFNLITIDTEDKSAWVQ 576
           S +Q T++C K  GLQIRTRSGGHD EG SY++ VPFV+VDL N + + ID   ++AWV+
Sbjct: 92  SHIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLRNMHSVKIDVHSQTAWVE 151

Query: 577 SGATV 582
           SGAT+
Sbjct: 152 SGATL 155

HSP 4 Score: 121.7 bits (304), Expect = 3.8e-26
Identity = 57/125 (45.60%), Postives = 83/125 (66.40%), Query Frame = 1

Query: 32  EDFVHCVYLHSNNNNDSNSTLIHIPSSSSYSHVLNFSIRNRRFSEAETPKPVAIITPSHA 91
           E+F+ C+  +   N  +N+ L++      Y  +LN +++N RF+   TPKP+ I TP + 
Sbjct: 32  ENFLKCLSQYIPTNV-TNAKLVYTQHDQFYMSILNSTVQNLRFTSDTTPKPLVITTPLNV 91

Query: 92  FQVQATVICCKSHGLQIRTRSGGHDFEGRSYVANVPFVLVDLINFNSITIDTEDKSAWVQ 151
             +Q T++C K  GLQIRTRSGGHD EG SY++ VPFV+VDL N +S+ ID   ++AWV+
Sbjct: 92  SHIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLRNMHSVKIDVHSQTAWVE 151

Query: 152 SGATV 157
           SGAT+
Sbjct: 152 SGATL 155

BLAST of ClCG03G002520 vs. Swiss-Prot
Match: CASL1_CANSA (Cannabidiolic acid synthase-like 1 OS=Cannabis sativa GN=CBDAS2 PE=2 SV=1)

HSP 1 Score: 211.5 bits (537), Expect = 3.7e-53
Identity = 105/252 (41.67%), Postives = 156/252 (61.90%), Query Frame = 1

Query: 156 VASNSSNGSGR---TIEASFFSLFLGNASELLALMEKIFPELGLKKEDCLEMSWVEAMAF 215
           +  N ++  G+   TI + F S+F G    L+ LM K FPELG+KK DC ++SW++ + F
Sbjct: 294 ITRNITDNQGKNKTTIHSYFSSIFHGGVDSLVDLMNKSFPELGIKKTDCKQLSWIDTIIF 353

Query: 216 SASGFVSAQSL----ELLLDRIPPKIGRNKIKSDYATQPISKIALEGMWERFKIEELETV 275
             SG V+  +     E+LLDR   +     IK DY  +PI + A+  + E+   E++   
Sbjct: 354 Y-SGVVNYNTTYFKKEILLDRSGGRKAAFSIKLDYVKKPIPETAMVTILEKLYEEDVGVG 413

Query: 276 QLILIPFGGKMNEISEAESPSPHRAGYPIHITYYVTWQQPDADSRHLEWTRELHDYMTPF 335
             +  P+GG M+EISE+  P PHRAG    I Y  +W++ + + +H+ W R ++++ TP+
Sbjct: 414 MFVFYPYGGIMDEISESAIPFPHRAGIMYEIWYIASWEKQEDNEKHINWIRNVYNFTTPY 473

Query: 336 VSKSPRAAYINHRDFDIGTNNEDGIPTSYEEASVWGQPYFGNNFERLMEVKRKVDPFNFF 395
           VS++PR AY+N+RD D+G  N +  P +Y +A +WG+ YFG NF RL++VK KVDP NFF
Sbjct: 474 VSQNPRMAYLNYRDLDLGKTNFES-PNNYTQARIWGEKYFGKNFNRLVKVKTKVDPDNFF 533

Query: 396 RHEQSIPPAPPR 401
           R+EQSIPP P R
Sbjct: 534 RNEQSIPPLPLR 543

HSP 2 Score: 196.8 bits (499), Expect = 9.4e-49
Identity = 100/237 (42.19%), Postives = 151/237 (63.71%), Query Frame = 1

Query: 592 TMEASFFSLFLGNASELLAHMEKIFPELGLKKEDCLEMSWVEAMAFSASGFVSAQSL--- 651
           T+ + F S+F G    L+  M K FPELG+KK DC ++SW++ + F  SG V+  +    
Sbjct: 308 TIHSYFSSIFHGGVDSLVDLMNKSFPELGIKKTDCKQLSWIDTIIFY-SGVVNYNTTYFK 367

Query: 652 -ELLLNRTPPKIGRYKTKSDYATQPISKTA----LEGMFKFEELETVRLVLIPFRGKMNE 711
            E+LL+R+  +   +  K DY  +PI +TA    LE +++ E++     V  P+ G M+E
Sbjct: 368 KEILLDRSGGRKAAFSIKLDYVKKPIPETAMVTILEKLYE-EDVGVGMFVFYPYGGIMDE 427

Query: 712 ISEVEIPSPHRAGYPIHIAYYVTWQRPDADSRHLEWMQELHDYMTPFVSKSPRAAYINCR 771
           ISE  IP PHRAG    I Y  +W++ + + +H+ W++ ++++ TP+VS++PR AY+N R
Sbjct: 428 ISESAIPFPHRAGIMYEIWYIASWEKQEDNEKHINWIRNVYNFTTPYVSQNPRMAYLNYR 487

Query: 772 DLDIGTNNKDGISMSYEEASVWGQPYFGNNFERLMEVKRKVDPFNFFRHEQSIPPAP 821
           DLD+G  N +  + +Y +A +WG+ YFG NF RL++VK KVDP NFFR+EQSIPP P
Sbjct: 488 DLDLGKTNFESPN-NYTQARIWGEKYFGKNFNRLVKVKTKVDPDNFFRNEQSIPPLP 541

HSP 3 Score: 125.6 bits (314), Expect = 2.7e-27
Identity = 59/125 (47.20%), Postives = 84/125 (67.20%), Query Frame = 1

Query: 457 EDFVHCVYLHSNNNNDSNSTLIHTPSSSSYSHVLNFSIRNRRFSETETPKPVAIITPSHA 516
           E+F+ C +      N +N+ L++T     Y  +LN +I+N RF+   TPKP+ IITP + 
Sbjct: 32  ENFLKC-FSQYIPTNVTNAKLVYTQHDQFYMSILNSTIQNLRFTSDTTPKPLVIITPLNV 91

Query: 517 SQVQATVICCKSHGLQIRTRSGGHDLEGRSYVANVPFVLVDLINFNLITIDTEDKSAWVQ 576
           S +Q T++C K  GLQIRTRSGGHD EG SY++ VPFV+VDL N + + ID   ++AWV+
Sbjct: 92  SHIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLRNMHSVKIDVHSQTAWVE 151

Query: 577 SGATV 582
           +GAT+
Sbjct: 152 AGATL 155

HSP 4 Score: 122.5 bits (306), Expect = 2.3e-26
Identity = 58/125 (46.40%), Postives = 83/125 (66.40%), Query Frame = 1

Query: 32  EDFVHCVYLHSNNNNDSNSTLIHIPSSSSYSHVLNFSIRNRRFSEAETPKPVAIITPSHA 91
           E+F+ C +      N +N+ L++      Y  +LN +I+N RF+   TPKP+ IITP + 
Sbjct: 32  ENFLKC-FSQYIPTNVTNAKLVYTQHDQFYMSILNSTIQNLRFTSDTTPKPLVIITPLNV 91

Query: 92  FQVQATVICCKSHGLQIRTRSGGHDFEGRSYVANVPFVLVDLINFNSITIDTEDKSAWVQ 151
             +Q T++C K  GLQIRTRSGGHD EG SY++ VPFV+VDL N +S+ ID   ++AWV+
Sbjct: 92  SHIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLRNMHSVKIDVHSQTAWVE 151

Query: 152 SGATV 157
           +GAT+
Sbjct: 152 AGATL 155

BLAST of ClCG03G002520 vs. TrEMBL
Match: A0A0A0LS77_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G096090 PE=4 SV=1)

HSP 1 Score: 405.6 bits (1041), Expect = 1.5e-109
Identity = 192/231 (83.12%), Postives = 212/231 (91.77%), Query Frame = 1

Query: 168 IEASFFSLFLGNASELLALMEKIFPELGLKKEDCLEMSWVEAMAFSASGFVSAQSLELLL 227
           +EASFFSLFLGNA+ELL+LMEK FPELGLKKEDCLE SWVE+MAFSASGFVSA+SLELLL
Sbjct: 1   MEASFFSLFLGNATELLSLMEKTFPELGLKKEDCLETSWVESMAFSASGFVSAKSLELLL 60

Query: 228 DRIPPKIGRNKIKSDYATQPISKIALEGMWERFKIEELETVQLILIPFGGKMNEISEAES 287
           DR P   GR K KSDYAT+PIS+  LEGMWERFK EELETVQLILIPFGGK NEISE+E+
Sbjct: 61  DRTPLHNGRYKTKSDYATEPISETVLEGMWERFKDEELETVQLILIPFGGKTNEISESET 120

Query: 288 PSPHRAGYPIHITYYVTWQQPDADSRHLEWTRELHDYMTPFVSKSPRAAYINHRDFDIGT 347
           PSPHRAGYPIHI YY+TWQ+PDADS+HL+W RELH+YMTPFVSKSPRAAY+N+RD D+GT
Sbjct: 121 PSPHRAGYPIHIGYYLTWQRPDADSKHLKWARELHNYMTPFVSKSPRAAYVNYRDLDMGT 180

Query: 348 NNEDGIPTSYEEASVWGQPYFGNNFERLMEVKRKVDPFNFFRHEQSIPPAP 399
           NN+DG+PT  EEAS+WG  YFGNNFERLMEVKRKVDPFNFFRHEQSIPPAP
Sbjct: 181 NNDDGVPTRCEEASIWGHRYFGNNFERLMEVKRKVDPFNFFRHEQSIPPAP 231

BLAST of ClCG03G002520 vs. TrEMBL
Match: A0A0A0LS77_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G096090 PE=4 SV=1)

HSP 1 Score: 386.3 bits (991), Expect = 9.3e-104
Identity = 188/231 (81.39%), Postives = 206/231 (89.18%), Query Frame = 1

Query: 593 MEASFFSLFLGNASELLAHMEKIFPELGLKKEDCLEMSWVEAMAFSASGFVSAQSLELLL 652
           MEASFFSLFLGNA+ELL+ MEK FPELGLKKEDCLE SWVE+MAFSASGFVSA+SLELLL
Sbjct: 1   MEASFFSLFLGNATELLSLMEKTFPELGLKKEDCLETSWVESMAFSASGFVSAKSLELLL 60

Query: 653 NRTPPKIGRYKTKSDYATQPISKTALEGM---FKFEELETVRLVLIPFRGKMNEISEVEI 712
           +RTP   GRYKTKSDYAT+PIS+T LEGM   FK EELETV+L+LIPF GK NEISE E 
Sbjct: 61  DRTPLHNGRYKTKSDYATEPISETVLEGMWERFKDEELETVQLILIPFGGKTNEISESET 120

Query: 713 PSPHRAGYPIHIAYYVTWQRPDADSRHLEWMQELHDYMTPFVSKSPRAAYINCRDLDIGT 772
           PSPHRAGYPIHI YY+TWQRPDADS+HL+W +ELH+YMTPFVSKSPRAAY+N RDLD+GT
Sbjct: 121 PSPHRAGYPIHIGYYLTWQRPDADSKHLKWARELHNYMTPFVSKSPRAAYVNYRDLDMGT 180

Query: 773 NNKDGISMSYEEASVWGQPYFGNNFERLMEVKRKVDPFNFFRHEQSIPPAP 821
           NN DG+    EEAS+WG  YFGNNFERLMEVKRKVDPFNFFRHEQSIPPAP
Sbjct: 181 NNDDGVPTRCEEASIWGHRYFGNNFERLMEVKRKVDPFNFFRHEQSIPPAP 231

HSP 2 Score: 306.2 bits (783), Expect = 1.2e-79
Identity = 178/412 (43.20%), Postives = 246/412 (59.71%), Query Frame = 1

Query: 10  YCFISLLLLFSLATLGCGDDNLEDFVHCVYLHSNNNNDSNSTLIHIPSSSSYSHVLNFSI 69
           + F   L  FS  T      + EDF+ C+ L S +++ S S +I+ P +SSY  VL   I
Sbjct: 9   FLFAVFLFSFSWVTTTSAHTH-EDFLECLKLQSEDSS-SISKVIYTPINSSYLSVLQLGI 68

Query: 70  RNRRFSEAETPKPVAIITPSHAFQVQATVICCKSHGLQIRTRSGGHDFEGRSYVANVPFV 129
            N RF+ + TPKP  I+TP +   ++AT+ C K H LQIR RSGGHD+EG SYV+  PFV
Sbjct: 69  NNERFNTSTTPKPQVIVTPLNLSHIEATITCSKKHDLQIRIRSGGHDYEGLSYVSPNPFV 128

Query: 130 LVDLINFNSITIDTEDKSAWVQSGAT-------VASNSSN-----------GSGRTIEAS 189
           ++DLI   SIT+D  +K+AWVQ GAT       +A  S N           G G      
Sbjct: 129 ILDLITLQSITVDATNKTAWVQVGATTGKLYYSIAEKSQNLAFPASVCTTVGIGGHFSGG 188

Query: 190 FFSLFL---GNASELLALMEKIFPELGLKKEDCLEMSWVEAMAFSASGFVSAQSLELLLD 249
            +   L   G A++ + +  ++    GL KED  EMSW+E++ + A GF S  SLE+LL+
Sbjct: 189 GYGTLLRKYGLAADNI-IDAQLTDVDGLTKEDSTEMSWIESVLYFA-GF-SNSSLEILLE 248

Query: 250 RIPPKIGRNKIKSDYATQPISKIALEGMWERFKIEELETVQLILIPFGGKMNEISEAESP 309
           R    +   K KSDY  QP+ ++ALEG+W+R    E+ + QL+   +GG+++EISE+  P
Sbjct: 249 RTQQNVRYFKAKSDYVQQPMPEVALEGIWKRLFEVEVGSGQLMFNLYGGRVSEISESSIP 308

Query: 310 SPHRAGYPIHITYYVTWQQPDADS--RHLEWTRELHDYMTPFVSKSPRAAYINHRDFDIG 369
            PHRAG    I + V W     +    H+ W R L+ ++ P+VSK+PR+AYIN+RD DIG
Sbjct: 309 FPHRAGNLYKIQHSVYWNDEGIEESITHISWIRRLYSFLAPYVSKNPRSAYINYRDLDIG 368

Query: 370 TNNEDGIPTSYEEASVWGQPYFGNNFERLMEVKRKVDPFNFFRHEQSIPPAP 399
            NN     TSYE+AS+WG  YF  NF+RL+ VK +VDP NFFR+EQSIP  P
Sbjct: 369 INNIGN--TSYEQASIWGIKYFKINFDRLVHVKTQVDPANFFRNEQSIPSLP 413

BLAST of ClCG03G002520 vs. TrEMBL
Match: A0A067KWX9_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_09769 PE=4 SV=1)

HSP 1 Score: 295.0 bits (754), Expect = 2.8e-76
Identity = 179/412 (43.45%), Postives = 247/412 (59.95%), Query Frame = 1

Query: 435 YCFISLLLLFSLATLGCGDDNLEDFVHCVYLHSNNNNDSNSTLIHTPSSSSYSHVLNFSI 494
           + F   L  FS  T      + EDF+ C+ L S +++ S S +I+TP +SSY  VL   I
Sbjct: 9   FLFAVFLFSFSWVTTTSAHTH-EDFLECLKLQSEDSS-SISKVIYTPINSSYLSVLQLGI 68

Query: 495 RNRRFSETETPKPVAIITPSHASQVQATVICCKSHGLQIRTRSGGHDLEGRSYVANVPFV 554
            N RF+ + TPKP  I+TP + S ++AT+ C K H LQIR RSGGHD EG SYV+  PFV
Sbjct: 69  NNERFNTSTTPKPQVIVTPLNLSHIEATITCSKKHDLQIRIRSGGHDYEGLSYVSPNPFV 128

Query: 555 LVDLINFNLITIDTEDKSAWVQSGAT-------VALNSSNG------------GGRTMEA 614
           ++DLI    IT+D  +K+AWVQ GAT       +A  S N             GG     
Sbjct: 129 ILDLITLQSITVDATNKTAWVQVGATTGKLYYSIAEKSQNLAFPASVCTTVGIGGHFSGG 188

Query: 615 SFFSLF--LGNASELLAHMEKIFPELGLKKEDCLEMSWVEAMAFSASGFVSAQSLELLLN 674
            + +L    G A++ +   +    + GL KED  EMSW+E++ + A GF S  SLE+LL 
Sbjct: 189 GYGTLLRKYGLAADNIIDAQLTDVD-GLTKEDSTEMSWIESVLYFA-GF-SNSSLEILLE 248

Query: 675 RTPPKIGRYKTKSDYATQPISKTALEGMFK--FE-ELETVRLVLIPFRGKMNEISEVEIP 734
           RT   +  +K KSDY  QP+ + ALEG++K  FE E+ + +L+   + G+++EISE  IP
Sbjct: 249 RTQQNVRYFKAKSDYVQQPMPEVALEGIWKRLFEVEVGSGQLMFNLYGGRVSEISESSIP 308

Query: 735 SPHRAGYPIHIAYYVTWQRPDADSR--HLEWMQELHDYMTPFVSKSPRAAYINCRDLDIG 794
            PHRAG    I + V W     +    H+ W++ L+ ++ P+VSK+PR+AYIN RDLDIG
Sbjct: 309 FPHRAGNLYKIQHSVYWNDEGIEESITHISWIRRLYSFLAPYVSKNPRSAYINYRDLDIG 368

Query: 795 TNNKDGISMSYEEASVWGQPYFGNNFERLMEVKRKVDPFNFFRHEQSIPPAP 821
            NN    + SYE+AS+WG  YF  NF+RL+ VK +VDP NFFR+EQSIP  P
Sbjct: 369 INNIG--NTSYEQASIWGIKYFKINFDRLVHVKTQVDPANFFRNEQSIPSLP 413

HSP 2 Score: 291.2 bits (744), Expect = 4.1e-75
Identity = 167/393 (42.49%), Postives = 231/393 (58.78%), Query Frame = 1

Query: 10  YCFISLLLLFSLATLGCGDDNLEDFVHCVYLHSNNNNDSNSTLIHIPSSSSYSHVLNFSI 69
           +  +++ LLFS A      D  ++FV C+Y + +N   S S  ++  ++SSYS  L+FSI
Sbjct: 8   FTVVAIALLFSFAY--STPDTHKNFVGCLYNYPHNTT-SISKAVYTQTNSSYSFTLDFSI 67

Query: 70  RNRRFSEAETPKPVAIITPSHAFQVQATVICCKSHGLQIRTRSGGHDFEGRSYVANVPFV 129
           RN RF    + KP+ I+TP     +QAT+IC + HG+QIRTRSGGHD+EG SYVA VPFV
Sbjct: 68  RNLRFYNVSS-KPLVIVTPLDVSHIQATIICSQRHGMQIRTRSGGHDYEGLSYVAEVPFV 127

Query: 130 LVDLINFNSITIDTEDKSAWVQSGATVASNSSNGSGRTIEASFFSLFLGNASELLALMEK 189
           +VDLIN   I +D E+ +AWVQ+GAT+               ++S  +   S+ L     
Sbjct: 128 VVDLINLREINVDVENSTAWVQAGATIGE------------LYYS--ISQKSKTLGFPAG 187

Query: 190 IFPELGL--KKEDCLEMSWVEAMAFSASGFVSAQSLEL---LLDRIPPKIGRNKIKSDYA 249
           + P +G             +     +A   + A  +++   +LDR          KSDY 
Sbjct: 188 MCPPVGTGGHFSGGGYGFLMRRYGLAADNIIDAHIIDVKGNVLDR------AGMAKSDYV 247

Query: 250 TQPISKIALEGMWERFKIEELETVQLILIPFGGKMNEISEAESPSPHRAGYPIHITYYVT 309
             PI  + L+ +W  F  +E +   +   P+GG+M+EISE+E P PHR+G   HI Y V 
Sbjct: 248 RDPIPDVGLQCLWPFFYEDEAQAAYVQFTPYGGRMDEISESEIPFPHRSGNIFHIQYGVE 307

Query: 310 WQQ--PDADSRHLEWTRELHDYMTPFVSKSPRAAYINHRDFDIGTNNEDGIPTSYEEASV 369
           WQ+   +A  RH+EW R ++ YM  +VSKSPRAAY+N+RD DIG NN DG  TSY +AS+
Sbjct: 308 WQEEGEEAAQRHVEWIRRVYSYMEAYVSKSPRAAYVNYRDLDIGVNN-DGY-TSYSQASI 367

Query: 370 WGQPYFGNNFERLMEVKRKVDPFNFFRHEQSIP 396
           WG  YF NNF RL  VK +VDP NFFR+EQSIP
Sbjct: 368 WGLKYFKNNFNRLARVKTRVDPLNFFRNEQSIP 374

BLAST of ClCG03G002520 vs. TrEMBL
Match: A0A151RZF5_CAJCA (Reticuline oxidase-like protein OS=Cajanus cajan GN=KK1_030435 PE=4 SV=1)

HSP 1 Score: 278.1 bits (710), Expect = 3.6e-71
Identity = 166/390 (42.56%), Postives = 229/390 (58.72%), Query Frame = 1

Query: 435 YCFISLLLLFSLATLGCGDDNLEDFVHCVYLHSNNNNDSNSTLIHTPSSSSYSHVLNFSI 494
           +  +++ LLFS A      D  ++FV C+Y + +N   S S  ++T ++SSYS  L+FSI
Sbjct: 8   FTVVAIALLFSFAY--STPDTHKNFVGCLYNYPHNTT-SISKAVYTQTNSSYSFTLDFSI 67

Query: 495 RNRRFSETETPKPVAIITPSHASQVQATVICCKSHGLQIRTRSGGHDLEGRSYVANVPFV 554
           RN RF    + KP+ I+TP   S +QAT+IC + HG+QIRTRSGGHD EG SYVA VPFV
Sbjct: 68  RNLRFYNVSS-KPLVIVTPLDVSHIQATIICSQRHGMQIRTRSGGHDYEGLSYVAEVPFV 127

Query: 555 LVDLINFNLITIDTEDKSAWVQSGATVALNSSNGGGRTMEASFFSLFLGNASELLAHMEK 614
           +VDLIN   I +D E+ +AWVQ+GAT+               ++S  +   S+ L     
Sbjct: 128 VVDLINLREINVDVENSTAWVQAGATIG------------ELYYS--ISQKSKTLGFPAG 187

Query: 615 IFPELGL--KKEDCLEMSWVEAMAFSASGFVSAQSLELLLNRTPPKIGRYKTKSDYATQP 674
           + P +G             +     +A   + A  +++  N    + G    KSDY   P
Sbjct: 188 MCPPVGTGGHFSGGGYGFLMRRYGLAADNIIDAHIIDVKGN-VLDRAG--MAKSDYVRDP 247

Query: 675 ISKTALEGMFKF---EELETVRLVLIPFRGKMNEISEVEIPSPHRAGYPIHIAYYVTWQR 734
           I    L+ ++ F   +E +   +   P+ G+M+EISE EIP PHR+G   HI Y V WQ 
Sbjct: 248 IPDVGLQCLWPFFYEDEAQAAYVQFTPYGGRMDEISESEIPFPHRSGNIFHIQYGVEWQE 307

Query: 735 --PDADSRHLEWMQELHDYMTPFVSKSPRAAYINCRDLDIGTNNKDGISMSYEEASVWGQ 794
              +A  RH+EW++ ++ YM  +VSKSPRAAY+N RDLDIG NN DG + SY +AS+WG 
Sbjct: 308 EGEEAAQRHVEWIRRVYSYMEAYVSKSPRAAYVNYRDLDIGVNN-DGYT-SYSQASIWGL 367

Query: 795 PYFGNNFERLMEVKRKVDPFNFFRHEQSIP 818
            YF NNF RL  VK +VDP NFFR+EQSIP
Sbjct: 368 KYFKNNFNRLARVKTRVDPLNFFRNEQSIP 374

HSP 2 Score: 271.2 bits (692), Expect = 4.4e-69
Identity = 132/240 (55.00%), Postives = 171/240 (71.25%), Query Frame = 1

Query: 159 NSSNGSGRTIEASFFSLFLGNASELLALMEKIFPELGLKKEDCLEMSWVEAMAFSASGFV 218
           NSS G   TI+A+F SLFLG    LL LM++ FPELGL K+DC+E+SW+E   F A GF 
Sbjct: 292 NSSQGGNPTIQATFTSLFLGGVDRLLPLMQESFPELGLVKDDCIELSWIEFALFLA-GFP 351

Query: 219 SAQSLELLLDRIPPKIGRNKIKSDYATQPISKIALEGMWERFKIEELETVQLILIPFGGK 278
           S  SL++LLDR P  I   K KSDY  QP+ + ALEGMWE F  +++E+  L ++P+GGK
Sbjct: 352 SNASLDVLLDRTPQSITSFKAKSDYVKQPLPETALEGMWETFFEKDIESPSLFMVPYGGK 411

Query: 279 MNEISEAESPSPHRAGYPIHITYYVTWQQ--PDADSRHLEWTRELHDYMTPFVSKSPRAA 338
           M EISE+  P PHRAG    I YYV W +   +A  RH+ W R L+ YMTP+VSK+PR A
Sbjct: 412 MEEISESSIPFPHRAGNLYKIHYYVAWTEEGKEASERHISWIRRLYSYMTPYVSKNPREA 471

Query: 339 YINHRDFDIGTNNEDGIPTSYEEASVWGQPYFGNNFERLMEVKRKVDPFNFFRHEQSIPP 397
           Y+N+RD D+G NN  G  TSY++AS+WG+ YF NNF++L+ +K +VDP NFFR+EQSIPP
Sbjct: 472 YVNYRDLDLGINNLAG-NTSYKQASIWGRKYFKNNFDKLVRIKTEVDPANFFRNEQSIPP 529

BLAST of ClCG03G002520 vs. TrEMBL
Match: U5FWX5_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0011s16570g PE=4 SV=1)

HSP 1 Score: 260.4 bits (664), Expect = 7.7e-66
Identity = 130/241 (53.94%), Postives = 175/241 (72.61%), Query Frame = 1

Query: 583 LNSSNGGGRTMEASFFSLFLGNASELLAHMEKIFPELGLKKEDCLEMSWVEAMAFSASGF 642
           +NSS GG  T++A+F SLFLG    LL  M++ FPELGL K+DC+E+SW+E   F A GF
Sbjct: 291 INSSQGGNPTIQATFTSLFLGGVDRLLPLMQESFPELGLVKDDCIELSWIEFALFLA-GF 350

Query: 643 VSAQSLELLLNRTPPKIGRYKTKSDYATQPISKTALEGMFK--FE-ELETVRLVLIPFRG 702
            S  SL++LL+RTP  I  +K KSDY  QP+ +TALEGM++  FE ++E+  L ++P+ G
Sbjct: 351 PSNASLDVLLDRTPQSITSFKAKSDYVKQPLPETALEGMWETFFEKDIESPSLFMVPYGG 410

Query: 703 KMNEISEVEIPSPHRAGYPIHIAYYVTW--QRPDADSRHLEWMQELHDYMTPFVSKSPRA 762
           KM EISE  IP PHRAG    I YYV W  +  +A  RH+ W++ L+ YMTP+VSK+PR 
Sbjct: 411 KMEEISESSIPFPHRAGNLYKIHYYVAWTEEGKEASERHISWIRRLYSYMTPYVSKNPRE 470

Query: 763 AYINCRDLDIGTNNKDGISMSYEEASVWGQPYFGNNFERLMEVKRKVDPFNFFRHEQSIP 819
           AY+N RDLD+G NN  G + SY++AS+WG+ YF NNF++L+ +K +VDP NFFR+EQSIP
Sbjct: 471 AYVNYRDLDLGINNLAG-NTSYKQASIWGRKYFKNNFDKLVRIKTEVDPANFFRNEQSIP 529

HSP 2 Score: 132.9 bits (333), Expect = 1.9e-27
Identity = 69/142 (48.59%), Postives = 100/142 (70.42%), Query Frame = 1

Query: 440 LLLLFSLATLGCGDDNLEDFVHCVYLHSNNNNDSNSTLIHTPSSSSYSHVLNFSIRNRRF 499
           L+LLF  +  G      EDF+ C+ L+S ++  + S +I+TP+++SY+ VL FSIRN RF
Sbjct: 13  LILLFPFSW-GTSAHTHEDFLQCLSLYSEDSA-ATSKVIYTPNNTSYATVLQFSIRNLRF 72

Query: 500 SETETPKPVAIITPSHASQVQATVICCKSHGLQIRTRSGGHDLEGRSYVANVPFVLVDLI 559
           + ++   PV I+TP+  S +QAT+ C + H LQIR RSGGHD EG SY++ +PFV++DLI
Sbjct: 73  NSSKLT-PVVIVTPTILSHIQATIHCSQKHNLQIRIRSGGHDYEGLSYMSVLPFVILDLI 132

Query: 560 NFNLITIDTEDKSAWVQSGATV 582
           N   IT+D   K+AWVQ+GAT+
Sbjct: 133 NLRKITVDLSTKTAWVQAGATL 151

HSP 3 Score: 130.6 bits (327), Expect = 9.2e-27
Identity = 67/142 (47.18%), Postives = 99/142 (69.72%), Query Frame = 1

Query: 15  LLLLFSLATLGCGDDNLEDFVHCVYLHSNNNNDSNSTLIHIPSSSSYSHVLNFSIRNRRF 74
           L+LLF  +  G      EDF+ C+ L+S ++  + S +I+ P+++SY+ VL FSIRN RF
Sbjct: 13  LILLFPFSW-GTSAHTHEDFLQCLSLYSEDSA-ATSKVIYTPNNTSYATVLQFSIRNLRF 72

Query: 75  SEAETPKPVAIITPSHAFQVQATVICCKSHGLQIRTRSGGHDFEGRSYVANVPFVLVDLI 134
           + ++   PV I+TP+    +QAT+ C + H LQIR RSGGHD+EG SY++ +PFV++DLI
Sbjct: 73  NSSKLT-PVVIVTPTILSHIQATIHCSQKHNLQIRIRSGGHDYEGLSYMSVLPFVILDLI 132

Query: 135 NFNSITIDTEDKSAWVQSGATV 157
           N   IT+D   K+AWVQ+GAT+
Sbjct: 133 NLRKITVDLSTKTAWVQAGATL 151

HSP 4 Score: 265.8 bits (678), Expect = 1.8e-67
Identity = 130/239 (54.39%), Postives = 174/239 (72.80%), Query Frame = 1

Query: 159 NSSNGSGRTIEASFFSLFLGNASELLALMEKIFPELGLKKEDCLEMSWVEAMAFSASGFV 218
           NSS     TI+A+FFSLFLG   +LL +M++ FPELGL K+DC EMSW+E++ F   GF 
Sbjct: 292 NSSQEGNTTIQATFFSLFLGEVDQLLPVMQESFPELGLVKDDCFEMSWIESV-FYTGGFT 351

Query: 219 SAQSLELLLDRIPPKIGRNKIKSDYATQPISKIALEGMWERFKIEELETVQLILIPFGGK 278
           S  SL++LL+R P  I R K KSDY  +P+ +IA EG+WERF  E++E   LILIP+GGK
Sbjct: 352 SNASLDVLLNRTPRSIPRFKAKSDYVKEPMPEIAFEGIWERFFEEDIEAPTLILIPYGGK 411

Query: 279 MNEISEAESPSPHRAGYPIHITYYVTWQQ--PDADSRHLEWTRELHDYMTPFVSKSPRAA 338
           M+EISE+ +P PHRAG    +   V+W++   +A  RH+ W R L+ Y+T +VSK+PR A
Sbjct: 412 MDEISESSTPFPHRAGNLYVLVSSVSWREESKEASRRHMAWIRRLYSYLTKYVSKNPREA 471

Query: 339 YINHRDFDIGTNNEDGIPTSYEEASVWGQPYFGNNFERLMEVKRKVDPFNFFRHEQSIP 396
           Y+N+RD D+G NN  G  TSY++AS+WG+ YF NNF+RL+ VK +VDP NFFR+EQSIP
Sbjct: 472 YVNYRDLDLGINNLTG-TTSYKQASIWGRKYFKNNFDRLVRVKTEVDPTNFFRNEQSIP 528

BLAST of ClCG03G002520 vs. TAIR10
Match: AT4G20860.1 (AT4G20860.1 FAD-binding Berberine family protein)

HSP 1 Score: 218.8 bits (556), Expect = 1.3e-56
Identity = 115/244 (47.13%), Postives = 160/244 (65.57%), Query Frame = 1

Query: 156 VASNSSNGSGRTIEASFFSLFLGNASELLALMEKIFPELGLKKEDCLEMSWVEAMAFSAS 215
           +  NS  G+ R ++++F +LFLG    L+ LM + FPELGL+ +DC EMSW+E++ F   
Sbjct: 291 IIDNSLEGNQRKVKSTFQTLFLGGIDRLIPLMNQKFPELGLRSQDCSEMSWIESIMFF-- 350

Query: 216 GFVSAQSLELLLDR-IPPKIGRNKIKSDYATQPISKIALEGMWERFKIEELETVQLILIP 275
            + S Q LE+LL+R +  +    K KSDY  +P+ +   E + +RF   E +T  +I  P
Sbjct: 351 NWRSGQPLEILLNRDLRFEDQYFKAKSDYVQKPVPENVFEEVTKRFL--EQDTPLMIFEP 410

Query: 276 FGGKMNEISEAESPSPHRAGYPIHITYYVTWQQPDAD--SRHLEWTRELHDYMTPFVSKS 335
            GGK+++ISE ESP PHR G   +I Y V W+  + +  ++H+ W R LHDYMTP+VSKS
Sbjct: 411 LGGKISKISETESPYPHRRGNLYNIQYMVKWKVNEVEEMNKHVRWMRSLHDYMTPYVSKS 470

Query: 336 PRAAYINHRDFDIGTNNEDGIPTSYEEASVWGQPYFGNNFERLMEVKRKVDPFNFFRHEQ 395
           PR AY+N+RD D+G+    GI TS+E+A  WG+ YF  NF+RL  VK K+DP NFFR+EQ
Sbjct: 471 PRGAYLNYRDLDLGSTK--GINTSFEDARKWGETYFKGNFKRLGLVKGKIDPTNFFRNEQ 528

Query: 396 SIPP 397
           SIPP
Sbjct: 531 SIPP 528

HSP 2 Score: 213.8 bits (543), Expect = 4.2e-55
Identity = 113/239 (47.28%), Postives = 155/239 (64.85%), Query Frame = 1

Query: 584 NSSNGGGRTMEASFFSLFLGNASELLAHMEKIFPELGLKKEDCLEMSWVEAMAFSASGFV 643
           NS  G  R ++++F +LFLG    L+  M + FPELGL+ +DC EMSW+E++ F    + 
Sbjct: 294 NSLEGNQRKVKSTFQTLFLGGIDRLIPLMNQKFPELGLRSQDCSEMSWIESIMFF--NWR 353

Query: 644 SAQSLELLLNRTPPKIGRY-KTKSDYATQPISKTALEGMFK-FEELETVRLVLIPFRGKM 703
           S Q LE+LLNR      +Y K KSDY  +P+ +   E + K F E +T  ++  P  GK+
Sbjct: 354 SGQPLEILLNRDLRFEDQYFKAKSDYVQKPVPENVFEEVTKRFLEQDTPLMIFEPLGGKI 413

Query: 704 NEISEVEIPSPHRAGYPIHIAYYVTWQRPDAD--SRHLEWMQELHDYMTPFVSKSPRAAY 763
           ++ISE E P PHR G   +I Y V W+  + +  ++H+ WM+ LHDYMTP+VSKSPR AY
Sbjct: 414 SKISETESPYPHRRGNLYNIQYMVKWKVNEVEEMNKHVRWMRSLHDYMTPYVSKSPRGAY 473

Query: 764 INCRDLDIGTNNKDGISMSYEEASVWGQPYFGNNFERLMEVKRKVDPFNFFRHEQSIPP 819
           +N RDLD+G+    GI+ S+E+A  WG+ YF  NF+RL  VK K+DP NFFR+EQSIPP
Sbjct: 474 LNYRDLDLGSTK--GINTSFEDARKWGETYFKGNFKRLGLVKGKIDPTNFFRNEQSIPP 528

HSP 3 Score: 92.0 bits (227), Expect = 1.8e-18
Identity = 52/156 (33.33%), Postives = 81/156 (51.92%), Query Frame = 1

Query: 435 YCFISLLLLFSLATLGCGDDNLEDFVHCVYLHSNNNNDSN---------STLIHTPSSSS 494
           + ++ LL L             E F +C+     N+   N          + +HT  S S
Sbjct: 5   FMYLFLLFLVLCVKSVYSTPTREQFQNCLSTKQFNSTLKNPINLTTHTLDSRVHTDFSES 64

Query: 495 YSHVLNFSIRNRRFSETETPKPVAIITPSHASQVQATVICCKSHGLQIRTRSGGHDLEGR 554
            S   N S  N  F+     KP+ I+ P   S+++ +++C +  G+Q+RT SGGHD EG 
Sbjct: 65  SSP--NSSFLNLNFTSL---KPILIVKPKSESEIKQSILCSRKLGVQVRTMSGGHDYEGL 124

Query: 555 SYVANVPFVLVDLINFNLITIDTEDKSAWVQSGATV 582
           SY++  PF++VDL+N   I+I+  D++AW+QSGAT+
Sbjct: 125 SYLSLSPFIIVDLVNLRSISINLTDETAWIQSGATL 155

HSP 4 Score: 91.3 bits (225), Expect = 3.1e-18
Identity = 51/156 (32.69%), Postives = 81/156 (51.92%), Query Frame = 1

Query: 10  YCFISLLLLFSLATLGCGDDNLEDFVHCVYLHSNNNNDSN---------STLIHIPSSSS 69
           + ++ LL L             E F +C+     N+   N          + +H   S S
Sbjct: 5   FMYLFLLFLVLCVKSVYSTPTREQFQNCLSTKQFNSTLKNPINLTTHTLDSRVHTDFSES 64

Query: 70  YSHVLNFSIRNRRFSEAETPKPVAIITPSHAFQVQATVICCKSHGLQIRTRSGGHDFEGR 129
            S   N S  N  F+     KP+ I+ P    +++ +++C +  G+Q+RT SGGHD+EG 
Sbjct: 65  SSP--NSSFLNLNFTSL---KPILIVKPKSESEIKQSILCSRKLGVQVRTMSGGHDYEGL 124

Query: 130 SYVANVPFVLVDLINFNSITIDTEDKSAWVQSGATV 157
           SY++  PF++VDL+N  SI+I+  D++AW+QSGAT+
Sbjct: 125 SYLSLSPFIIVDLVNLRSISINLTDETAWIQSGATL 155

BLAST of ClCG03G002520 vs. TAIR10
Match: AT5G44400.1 (AT5G44400.1 FAD-binding Berberine family protein)

HSP 1 Score: 210.3 bits (534), Expect = 4.6e-54
Identity = 111/238 (46.64%), Postives = 154/238 (64.71%), Query Frame = 1

Query: 585 SSNGGGRTMEASFFSLFLGNASELLAHMEKIFPELGLKKEDCLEMSWVEAMAFSASGFVS 644
           +  GG +T+  S+ +LFLG    L+  M+K FPELGL  +DC+EMSW+E++A+  SGF +
Sbjct: 301 AGTGGNKTVTTSYNALFLGGKGTLMNVMKKSFPELGLTFKDCIEMSWLESIAY-ISGFPT 360

Query: 645 AQSLELLL-NRTP-PKIGRYKTKSDYATQPISKTALEGMFKFEELETVRLVL-IPFRGKM 704
                +LL  ++P PK+  +K KSD+   PI ++ L+G+FK    E + L++  P+ G M
Sbjct: 361 HTPTNVLLQGKSPFPKVS-FKAKSDFVKTPIPESGLQGIFKKLLKEDIPLMIWNPYGGMM 420

Query: 705 NEISEVEIPSPHRAGYPIHIAYYVTWQRPD-ADSRHLEWMQELHDYMTPFVSKSPRAAYI 764
            +I E +IP PHR G    + Y  +W   D   SRH+ W+++L+ YMTP+VS +PR AY+
Sbjct: 421 AKIPESQIPFPHRKGVLFKVQYVTSWLDSDKRPSRHINWIRDLYSYMTPYVSSNPREAYV 480

Query: 765 NCRDLDIGTNNKDGISMSYEEASVWGQPYFGNNFERLMEVKRKVDPFNFFRHEQSIPP 819
           N RDLD+G N KD +    ++A VWG  YF NNF RLM +K KVDP NFFRHEQSIPP
Sbjct: 481 NYRDLDLGRNTKD-VKTCIKQAQVWGANYFKNNFNRLMMIKAKVDPENFFRHEQSIPP 535

HSP 2 Score: 208.0 bits (528), Expect = 2.3e-53
Identity = 108/240 (45.00%), Postives = 153/240 (63.75%), Query Frame = 1

Query: 160 SSNGSGRTIEASFFSLFLGNASELLALMEKIFPELGLKKEDCLEMSWVEAMAFSASGFVS 219
           +  G  +T+  S+ +LFLG    L+ +M+K FPELGL  +DC+EMSW+E++A+  SGF +
Sbjct: 301 AGTGGNKTVTTSYNALFLGGKGTLMNVMKKSFPELGLTFKDCIEMSWLESIAY-ISGFPT 360

Query: 220 AQSLELLLD-RIP-PKIGRNKIKSDYATQPISKIALEGMWERFKIEELETVQLILIPFGG 279
                +LL  + P PK+   K KSD+   PI +  L+G++++   E++    +I  P+GG
Sbjct: 361 HTPTNVLLQGKSPFPKVS-FKAKSDFVKTPIPESGLQGIFKKLLKEDIPL--MIWNPYGG 420

Query: 280 KMNEISEAESPSPHRAGYPIHITYYVTWQQPD-ADSRHLEWTRELHDYMTPFVSKSPRAA 339
            M +I E++ P PHR G    + Y  +W   D   SRH+ W R+L+ YMTP+VS +PR A
Sbjct: 421 MMAKIPESQIPFPHRKGVLFKVQYVTSWLDSDKRPSRHINWIRDLYSYMTPYVSSNPREA 480

Query: 340 YINHRDFDIGTNNEDGIPTSYEEASVWGQPYFGNNFERLMEVKRKVDPFNFFRHEQSIPP 397
           Y+N+RD D+G N +D + T  ++A VWG  YF NNF RLM +K KVDP NFFRHEQSIPP
Sbjct: 481 YVNYRDLDLGRNTKD-VKTCIKQAQVWGANYFKNNFNRLMMIKAKVDPENFFRHEQSIPP 535

HSP 3 Score: 104.8 bits (260), Expect = 2.7e-22
Identity = 63/151 (41.72%), Postives = 87/151 (57.62%), Query Frame = 1

Query: 437 FISLLLLFSLATLGCGDD--NLED-FVHCVYLHSNNNNDSNSTLIH-TPSSSSYSHVLNF 496
           F  L+L FSL T+       +L+D F++CV  +++       T    T + S +S VL  
Sbjct: 10  FSILVLYFSLYTITPTSSLASLQDQFINCVQRNTHVYFPLEKTFFAPTKNVSMFSQVLES 69

Query: 497 SIRNRRFSETETPKPVAIITPSHASQVQATVICCKSHGLQIRTRSGGHDLEGRSYVANV- 556
           + +N RF +   PKP  I +P H S VQA++IC K   + +R RSGGHD EG SYV+ + 
Sbjct: 70  TAQNLRFLKKSMPKPGFIFSPIHESHVQASIICSKKLRMHLRVRSGGHDYEGLSYVSQID 129

Query: 557 -PFVLVDLINFNLITIDTEDKSAWVQSGATV 582
            PF+L+DL     + I+ +D SAWVQSGATV
Sbjct: 130 KPFILMDLSKMRQVNINIQDNSAWVQSGATV 160

HSP 4 Score: 102.4 bits (254), Expect = 1.4e-21
Identity = 61/151 (40.40%), Postives = 86/151 (56.95%), Query Frame = 1

Query: 12  FISLLLLFSLATLGCGDD--NLED-FVHCVYLHSNNNNDSNSTLIHIPSS-SSYSHVLNF 71
           F  L+L FSL T+       +L+D F++CV  +++       T      + S +S VL  
Sbjct: 10  FSILVLYFSLYTITPTSSLASLQDQFINCVQRNTHVYFPLEKTFFAPTKNVSMFSQVLES 69

Query: 72  SIRNRRFSEAETPKPVAIITPSHAFQVQATVICCKSHGLQIRTRSGGHDFEGRSYVANV- 131
           + +N RF +   PKP  I +P H   VQA++IC K   + +R RSGGHD+EG SYV+ + 
Sbjct: 70  TAQNLRFLKKSMPKPGFIFSPIHESHVQASIICSKKLRMHLRVRSGGHDYEGLSYVSQID 129

Query: 132 -PFVLVDLINFNSITIDTEDKSAWVQSGATV 157
            PF+L+DL     + I+ +D SAWVQSGATV
Sbjct: 130 KPFILMDLSKMRQVNINIQDNSAWVQSGATV 160

BLAST of ClCG03G002520 vs. TAIR10
Match: AT5G44380.1 (AT5G44380.1 FAD-binding Berberine family protein)

HSP 1 Score: 208.0 bits (528), Expect = 2.3e-53
Identity = 109/240 (45.42%), Postives = 146/240 (60.83%), Query Frame = 1

Query: 585 SSNGGGRTMEASFFSLFLGNASELLAHMEKIFPELGLKKEDCLEMSWVEAMAFSASGFVS 644
           + N G +T+  ++   FLG    LL  MEK FPELGL ++DC EMSW+EA  F   GF +
Sbjct: 304 AGNDGNKTVTMTYLGQFLGEKGTLLKVMEKAFPELGLTQKDCTEMSWIEAALFH-GGFPT 363

Query: 645 AQSLELLLNRTPPKIGR--YKTKSDYATQPISKTALEGMFK-FEELETVRLVLIPFRGKM 704
              +E+LL    P +G+  +K  SD+  +PI    L+G+FK   E  T  L   P+ G M
Sbjct: 364 GSPIEILLQLKSP-LGKDYFKATSDFVKEPIPVIGLKGIFKRLIEGNTTFLNWTPYGGMM 423

Query: 705 NEISEVEIPSPHRAGYPIHIAYYVTWQRPD-ADSRHLEWMQELHDYMTPFVSKSPRAAYI 764
           ++I E  IP PHR G    I YY  W   D   SR + W++E+++YM P+VS +PR AY+
Sbjct: 424 SKIPESAIPFPHRNGTLFKILYYANWLENDKTSSRKINWIKEIYNYMAPYVSSNPRQAYV 483

Query: 765 NCRDLDIGTNNKDGISMSYEEASVWGQPYFGNNFERLMEVKRKVDPFNFFRHEQSIPPAP 821
           N RDLD G  NK+   +++ EA +WG  YF  NF+RL+++K KVDP NFFRHEQSIPP P
Sbjct: 484 NYRDLDFG-QNKNNAKVNFIEAKIWGPKYFKGNFDRLVKIKTKVDPENFFRHEQSIPPMP 540

HSP 2 Score: 206.8 bits (525), Expect = 5.1e-53
Identity = 106/242 (43.80%), Postives = 149/242 (61.57%), Query Frame = 1

Query: 160 SSNGSGRTIEASFFSLFLGNASELLALMEKIFPELGLKKEDCLEMSWVEAMAFSASGFVS 219
           + N   +T+  ++   FLG    LL +MEK FPELGL ++DC EMSW+EA  F   GF +
Sbjct: 304 AGNDGNKTVTMTYLGQFLGEKGTLLKVMEKAFPELGLTQKDCTEMSWIEAALFHG-GFPT 363

Query: 220 AQSLELLLDRIPPKIGRNKIK--SDYATQPISKIALEGMWERFKIEELETVQLILIPFGG 279
              +E+LL    P +G++  K  SD+  +PI  I L+G+++R  + E  T  L   P+GG
Sbjct: 364 GSPIEILLQLKSP-LGKDYFKATSDFVKEPIPVIGLKGIFKR--LIEGNTTFLNWTPYGG 423

Query: 280 KMNEISEAESPSPHRAGYPIHITYYVTWQQPD-ADSRHLEWTRELHDYMTPFVSKSPRAA 339
            M++I E+  P PHR G    I YY  W + D   SR + W +E+++YM P+VS +PR A
Sbjct: 424 MMSKIPESAIPFPHRNGTLFKILYYANWLENDKTSSRKINWIKEIYNYMAPYVSSNPRQA 483

Query: 340 YINHRDFDIGTNNEDGIPTSYEEASVWGQPYFGNNFERLMEVKRKVDPFNFFRHEQSIPP 399
           Y+N+RD D G  N++    ++ EA +WG  YF  NF+RL+++K KVDP NFFRHEQSIPP
Sbjct: 484 YVNYRDLDFG-QNKNNAKVNFIEAKIWGPKYFKGNFDRLVKIKTKVDPENFFRHEQSIPP 540

HSP 3 Score: 100.1 bits (248), Expect = 6.8e-21
Identity = 61/153 (39.87%), Postives = 84/153 (54.90%), Query Frame = 1

Query: 436 CFISLLLLFSL--ATLGCGDDNLED-FVHCVYLHSNNNNDSNSTLIHTPSS--SSYSHVL 495
           C I  +L FS    T      +++D F++CV  +++ +     TL  TP+   S ++ VL
Sbjct: 12  CIIVSVLYFSFYCITPTSSSASIQDQFINCVKRNTHVSFPLEKTLF-TPAKNVSLFNQVL 71

Query: 496 NFSIRNRRFSETETPKPVAIITPSHASQVQATVICCKSHGLQIRTRSGGHDLEGRSYVAN 555
             + +N +F     PKP  I  P H SQVQA++IC K  G+  R RSGGHD E  SYV+ 
Sbjct: 72  ESTAQNLQFLAKSMPKPGFIFRPIHQSQVQASIICSKKLGIHFRVRSGGHDFEALSYVSR 131

Query: 556 V--PFVLVDLINFNLITIDTEDKSAWVQSGATV 582
           +  PF+L+DL     I +D E  SAWVQ GAT+
Sbjct: 132 IEKPFILLDLSKLKQINVDIESNSAWVQPGATL 163

HSP 4 Score: 99.0 bits (245), Expect = 1.5e-20
Identity = 59/152 (38.82%), Postives = 82/152 (53.95%), Query Frame = 1

Query: 11  CFISLLLLFSL--ATLGCGDDNLED-FVHCVYLHSNNNNDSNSTLIHIPSSSS-YSHVLN 70
           C I  +L FS    T      +++D F++CV  +++ +     TL     + S ++ VL 
Sbjct: 12  CIIVSVLYFSFYCITPTSSSASIQDQFINCVKRNTHVSFPLEKTLFTPAKNVSLFNQVLE 71

Query: 71  FSIRNRRFSEAETPKPVAIITPSHAFQVQATVICCKSHGLQIRTRSGGHDFEGRSYVANV 130
            + +N +F     PKP  I  P H  QVQA++IC K  G+  R RSGGHDFE  SYV+ +
Sbjct: 72  STAQNLQFLAKSMPKPGFIFRPIHQSQVQASIICSKKLGIHFRVRSGGHDFEALSYVSRI 131

Query: 131 --PFVLVDLINFNSITIDTEDKSAWVQSGATV 157
             PF+L+DL     I +D E  SAWVQ GAT+
Sbjct: 132 EKPFILLDLSKLKQINVDIESNSAWVQPGATL 163

BLAST of ClCG03G002520 vs. TAIR10
Match: AT5G44390.1 (AT5G44390.1 FAD-binding Berberine family protein)

HSP 1 Score: 207.2 bits (526), Expect = 3.9e-53
Identity = 109/244 (44.67%), Postives = 158/244 (64.75%), Query Frame = 1

Query: 160 SSNGSGRTIEASFFSLFLGNASELLALMEKIFPELGLKKEDCLEMSWVEAMAFSASGFVS 219
           S N + +T+  ++   FLG    L+ +M+K FPELGL ++DC+EMSW++++ ++ SGF +
Sbjct: 303 SGNKANKTVTMAYIGQFLGEKGTLMEVMKKDFPELGLTQKDCIEMSWIDSIIYN-SGFPT 362

Query: 220 --AQSLELLLDRIPPKIGRN--KIKSDYATQPISKIALEGMWERFKIEELETVQLILIPF 279
                +E+LL    P IG+   K KSD+A +PI  + LEGM++  K+ E +   +I  P+
Sbjct: 363 NPPPPIEILLQAKSP-IGKVYFKGKSDFAKKPIPVLGLEGMFK--KLLEEDAALVIWTPY 422

Query: 280 GGKMNEISEAESPSPHRAGYPIHITYYVTWQQPDA-DSRHLEWTRELHDYMTPFVSKSPR 339
           GGKM++I E+E P PHR G    I YY +W   +   +R  +W REL+ YMTP+VS +PR
Sbjct: 423 GGKMDKIPESEIPFPHRNGTNFMIQYYRSWSDSEKRPNRRTKWIRELYGYMTPYVSSNPR 482

Query: 340 AAYINHRDFDIGTNNEDGIPTSYEEASVWGQPYFGNNFERLMEVKRKVDPFNFFRHEQSI 399
            AY+N+RD D+G  N+D   +++ EA +WG  YF +NF RL+ +K KVDP NFFRHEQSI
Sbjct: 483 QAYVNYRDLDLG-QNKDNSKSNFIEAKIWGANYFKDNFNRLVRIKSKVDPDNFFRHEQSI 541

HSP 2 Score: 205.7 bits (522), Expect = 1.1e-52
Identity = 112/242 (46.28%), Postives = 153/242 (63.22%), Query Frame = 1

Query: 585 SSNGGGRTMEASFFSLFLGNASELLAHMEKIFPELGLKKEDCLEMSWVEAMAFSASGFVS 644
           S N   +T+  ++   FLG    L+  M+K FPELGL ++DC+EMSW++++ ++ SGF +
Sbjct: 303 SGNKANKTVTMAYIGQFLGEKGTLMEVMKKDFPELGLTQKDCIEMSWIDSIIYN-SGFPT 362

Query: 645 --AQSLELLLNRTPPKIGR--YKTKSDYATQPISKTALEGMFKFEELETVRLVL-IPFRG 704
                +E+LL    P IG+  +K KSD+A +PI    LEGMFK    E   LV+  P+ G
Sbjct: 363 NPPPPIEILLQAKSP-IGKVYFKGKSDFAKKPIPVLGLEGMFKKLLEEDAALVIWTPYGG 422

Query: 705 KMNEISEVEIPSPHRAGYPIHIAYYVTWQRPDA-DSRHLEWMQELHDYMTPFVSKSPRAA 764
           KM++I E EIP PHR G    I YY +W   +   +R  +W++EL+ YMTP+VS +PR A
Sbjct: 423 KMDKIPESEIPFPHRNGTNFMIQYYRSWSDSEKRPNRRTKWIRELYGYMTPYVSSNPRQA 482

Query: 765 YINCRDLDIGTNNKDGISMSYEEASVWGQPYFGNNFERLMEVKRKVDPFNFFRHEQSIPP 821
           Y+N RDLD+G  NKD    ++ EA +WG  YF +NF RL+ +K KVDP NFFRHEQSIP 
Sbjct: 483 YVNYRDLDLG-QNKDNSKSNFIEAKIWGANYFKDNFNRLVRIKSKVDPDNFFRHEQSIPT 541

HSP 3 Score: 105.5 bits (262), Expect = 1.6e-22
Identity = 60/147 (40.82%), Postives = 82/147 (55.78%), Query Frame = 1

Query: 439 SLLLLFSLATLGCGDDNLEDFVHCVYLHSNNNNDSNSTLIHTP--SSSSYSHVLNFSIRN 498
           +L   F   TL       +DF+ C+Y ++N     + T   TP  ++S ++ VL  + +N
Sbjct: 17  ALYFSFYTITLTSSTSLQDDFIKCLYRNTNVRFTLDKTFF-TPERNASIFTEVLESTAQN 76

Query: 499 RRFSETETPKPVAIITPSHASQVQATVICCKSHGLQIRTRSGGHDLEGRSYVANV--PFV 558
           +R+     PKP  I  P H S VQA+VIC K   +  R RSGGHD EG SYV+ +  PFV
Sbjct: 77  QRYLTKTMPKPGFIFKPVHESHVQASVICSKKLEIHFRVRSGGHDYEGVSYVSQIEKPFV 136

Query: 559 LVDLINFNLITIDTEDKSAWVQSGATV 582
           L+DL     I +D +D SAWV++GATV
Sbjct: 137 LIDLSKLRQINVDIKDTSAWVEAGATV 162

HSP 4 Score: 104.0 bits (258), Expect = 4.7e-22
Identity = 57/146 (39.04%), Postives = 80/146 (54.79%), Query Frame = 1

Query: 14  SLLLLFSLATLGCGDDNLEDFVHCVYLHSNNNNDSNSTLIHIPSSSS-YSHVLNFSIRNR 73
           +L   F   TL       +DF+ C+Y ++N     + T      ++S ++ VL  + +N+
Sbjct: 17  ALYFSFYTITLTSSTSLQDDFIKCLYRNTNVRFTLDKTFFTPERNASIFTEVLESTAQNQ 76

Query: 74  RFSEAETPKPVAIITPSHAFQVQATVICCKSHGLQIRTRSGGHDFEGRSYVANV--PFVL 133
           R+     PKP  I  P H   VQA+VIC K   +  R RSGGHD+EG SYV+ +  PFVL
Sbjct: 77  RYLTKTMPKPGFIFKPVHESHVQASVICSKKLEIHFRVRSGGHDYEGVSYVSQIEKPFVL 136

Query: 134 VDLINFNSITIDTEDKSAWVQSGATV 157
           +DL     I +D +D SAWV++GATV
Sbjct: 137 IDLSKLRQINVDIKDTSAWVEAGATV 162

BLAST of ClCG03G002520 vs. TAIR10
Match: AT5G44360.1 (AT5G44360.1 FAD-binding Berberine family protein)

HSP 1 Score: 205.3 bits (521), Expect = 1.5e-52
Identity = 109/241 (45.23%), Postives = 151/241 (62.66%), Query Frame = 1

Query: 164 SGRTIEASFFSLFLGNASELLALMEKIFPELGLKKEDCLEMSWVEAMAFSASGFVSAQSL 223
           SG +++ +F + +LG   +L+ LM + FPELGL  +DC EM+W++++ +    +   Q L
Sbjct: 300 SGGSVQTTFQANYLGGIDKLIPLMNQKFPELGLTFQDCSEMTWIDSIMYF--NWKKGQPL 359

Query: 224 ELLLDRIPPKIGRN------KIKSDYATQPISKIALEGMWERFKIEELETVQLILIPFGG 283
           E LLDR     G+       K KSD+   PI +I LEG+W RF   E+E+  +I+ P GG
Sbjct: 360 ETLLDR-----GQRYNDLYFKAKSDFVKNPIPEIGLEGIWTRF--HEVESPIMIMEPLGG 419

Query: 284 KMNEISEAESPSPHRAGYPIHITYYVTWQQPDAD--SRHLEWTRELHDYMTPFVSKSPRA 343
           KM EI E E+P PHR G   +I Y V W+  D     +H+ W R L+ YM  +VS SPR 
Sbjct: 420 KMYEIGETETPFPHRRGNLYNIQYMVKWRLKDIGVMEKHVTWMRLLYRYMRVYVSASPRG 479

Query: 344 AYINHRDFDIGTNNEDGIPTSYEEASVWGQPYFGNNFERLMEVKRKVDPFNFFRHEQSIP 397
           AY+N+RD D+G N   G+ TS+E+A +WG  YFG+NF+RL  VK K+DP NFFR+EQS+P
Sbjct: 480 AYLNYRDLDLGMNR--GVNTSFEDAKLWGFRYFGSNFKRLAIVKGKIDPTNFFRNEQSVP 529

HSP 2 Score: 191.0 bits (484), Expect = 2.9e-48
Identity = 101/233 (43.35%), Postives = 146/233 (62.66%), Query Frame = 1

Query: 590 GRTMEASFFSLFLGNASELLAHMEKIFPELGLKKEDCLEMSWVEAMAFSASGFVSAQSLE 649
           G +++ +F + +LG   +L+  M + FPELGL  +DC EM+W++++ +    +   Q LE
Sbjct: 301 GGSVQTTFQANYLGGIDKLIPLMNQKFPELGLTFQDCSEMTWIDSIMYF--NWKKGQPLE 360

Query: 650 LLLNRTPPKIGRY-KTKSDYATQPISKTALEGMF-KFEELETVRLVLIPFRGKMNEISEV 709
            LL+R       Y K KSD+   PI +  LEG++ +F E+E+  +++ P  GKM EI E 
Sbjct: 361 TLLDRGQRYNDLYFKAKSDFVKNPIPEIGLEGIWTRFHEVESPIMIMEPLGGKMYEIGET 420

Query: 710 EIPSPHRAGYPIHIAYYVTWQRPDAD--SRHLEWMQELHDYMTPFVSKSPRAAYINCRDL 769
           E P PHR G   +I Y V W+  D     +H+ WM+ L+ YM  +VS SPR AY+N RDL
Sbjct: 421 ETPFPHRRGNLYNIQYMVKWRLKDIGVMEKHVTWMRLLYRYMRVYVSASPRGAYLNYRDL 480

Query: 770 DIGTNNKDGISMSYEEASVWGQPYFGNNFERLMEVKRKVDPFNFFRHEQSIPP 819
           D+G N   G++ S+E+A +WG  YFG+NF+RL  VK K+DP NFFR+EQS+PP
Sbjct: 481 DLGMNR--GVNTSFEDAKLWGFRYFGSNFKRLAIVKGKIDPTNFFRNEQSVPP 529

HSP 3 Score: 95.1 bits (235), Expect = 2.2e-19
Identity = 51/151 (33.77%), Postives = 90/151 (59.60%), Query Frame = 1

Query: 13  ISLLLLFSLATLGCGDDNL---EDFVHCVYLHSNNNNDSNSTLIHIPSSSSYSHVLNFSI 72
           +SL L+F +  +     +    + F+ C+  HS+++  +  + IH P S  Y+      I
Sbjct: 9   LSLFLVFLVKWVNSDSSSSPSKDQFLSCMSTHSDSSFINPKSFIHKPDSRVYTDFSQSLI 68

Query: 73  -RNRRFSEAE--TPKPVAIITPSHAFQVQATVICCKSHGLQIRTRSGGHDFEGRSYVA-N 132
            +N RF      + KP+ I+TP    ++Q +++C +  G+++RT+SGGHD+EG SY++ +
Sbjct: 69  SQNYRFLTLNFTSQKPILIVTPRTDTEIQRSLLCSRKLGVKVRTKSGGHDYEGLSYLSLH 128

Query: 133 VPFVLVDLINFNSITIDTEDKSAWVQSGATV 157
            PF+++DL+N  SI I+  D++AWV +GAT+
Sbjct: 129 SPFIILDLVNVRSIEINLADETAWVGAGATI 159

HSP 4 Score: 93.2 bits (230), Expect = 8.3e-19
Identity = 50/151 (33.11%), Postives = 89/151 (58.94%), Query Frame = 1

Query: 438 ISLLLLFSLATLGCGDDNL---EDFVHCVYLHSNNNNDSNSTLIHTPSSSSYSHVLNFSI 497
           +SL L+F +  +     +    + F+ C+  HS+++  +  + IH P S  Y+      I
Sbjct: 9   LSLFLVFLVKWVNSDSSSSPSKDQFLSCMSTHSDSSFINPKSFIHKPDSRVYTDFSQSLI 68

Query: 498 -RNRRFSETE--TPKPVAIITPSHASQVQATVICCKSHGLQIRTRSGGHDLEGRSYVA-N 557
            +N RF      + KP+ I+TP   +++Q +++C +  G+++RT+SGGHD EG SY++ +
Sbjct: 69  SQNYRFLTLNFTSQKPILIVTPRTDTEIQRSLLCSRKLGVKVRTKSGGHDYEGLSYLSLH 128

Query: 558 VPFVLVDLINFNLITIDTEDKSAWVQSGATV 582
            PF+++DL+N   I I+  D++AWV +GAT+
Sbjct: 129 SPFIILDLVNVRSIEINLADETAWVGAGATI 159

BLAST of ClCG03G002520 vs. NCBI nr
Match: gi|659072076|ref|XP_008463307.1| (PREDICTED: tetrahydrocannabinolic acid synthase-like [Cucumis melo])

HSP 1 Score: 426.4 bits (1095), Expect = 1.2e-115
Identity = 202/256 (78.91%), Postives = 230/256 (89.84%), Query Frame = 1

Query: 145 DKSAWVQSGATVASNSSNGSGRTIEASFFSLFLGNASELLALMEKIFPELGLKKEDCLEM 204
           D+  ++ +  T +++S + SGR +EASFFSLFLGNA+ELL+LMEK FPELGLKK+DCLEM
Sbjct: 283 DQDLFISAWVTASNSSKDESGRIMEASFFSLFLGNATELLSLMEKTFPELGLKKKDCLEM 342

Query: 205 SWVEAMAFSASGFVSAQSLELLLDRIPPKIGRNKIKSDYATQPISKIALEGMWERFKIEE 264
           SWVE+MAFSASGFV A+SLELLLDR P   GR K KSDYAT+PISK AL+GMWERFK EE
Sbjct: 343 SWVESMAFSASGFVYAKSLELLLDRTPVDNGRYKTKSDYATEPISKTALKGMWERFKDEE 402

Query: 265 LETVQLILIPFGGKMNEISEAESPSPHRAGYPIHITYYVTWQQPDADSRHLEWTRELHDY 324
           LETVQLILIPFGGKMNEISE+E+PSPHRAGYPIHI YY+TWQQP+ADSR+LEW RELHDY
Sbjct: 403 LETVQLILIPFGGKMNEISESETPSPHRAGYPIHIGYYLTWQQPNADSRNLEWARELHDY 462

Query: 325 MTPFVSKSPRAAYINHRDFDIGTNNEDGIPTSYEEASVWGQPYFGNNFERLMEVKRKVDP 384
           MTPFVSKSPRAAY+N+RD D+GTNN+DG+PT Y+EAS+WG  YFGNNFERLMEVK KVDP
Sbjct: 463 MTPFVSKSPRAAYVNYRDLDMGTNNDDGVPTRYKEASLWGHRYFGNNFERLMEVKMKVDP 522

Query: 385 FNFFRHEQSIPPAPPR 401
           FNFFRHEQSIPPAPPR
Sbjct: 523 FNFFRHEQSIPPAPPR 538

BLAST of ClCG03G002520 vs. NCBI nr
Match: gi|659072076|ref|XP_008463307.1| (PREDICTED: tetrahydrocannabinolic acid synthase-like [Cucumis melo])

HSP 1 Score: 399.1 bits (1024), Expect = 2.0e-107
Identity = 194/255 (76.08%), Postives = 221/255 (86.67%), Query Frame = 1

Query: 570 DKSAWVQSGATVALNSSNGGGRTMEASFFSLFLGNASELLAHMEKIFPELGLKKEDCLEM 629
           D+  ++ +  T + +S +  GR MEASFFSLFLGNA+ELL+ MEK FPELGLKK+DCLEM
Sbjct: 283 DQDLFISAWVTASNSSKDESGRIMEASFFSLFLGNATELLSLMEKTFPELGLKKKDCLEM 342

Query: 630 SWVEAMAFSASGFVSAQSLELLLNRTPPKIGRYKTKSDYATQPISKTALEGM---FKFEE 689
           SWVE+MAFSASGFV A+SLELLL+RTP   GRYKTKSDYAT+PISKTAL+GM   FK EE
Sbjct: 343 SWVESMAFSASGFVYAKSLELLLDRTPVDNGRYKTKSDYATEPISKTALKGMWERFKDEE 402

Query: 690 LETVRLVLIPFRGKMNEISEVEIPSPHRAGYPIHIAYYVTWQRPDADSRHLEWMQELHDY 749
           LETV+L+LIPF GKMNEISE E PSPHRAGYPIHI YY+TWQ+P+ADSR+LEW +ELHDY
Sbjct: 403 LETVQLILIPFGGKMNEISESETPSPHRAGYPIHIGYYLTWQQPNADSRNLEWARELHDY 462

Query: 750 MTPFVSKSPRAAYINCRDLDIGTNNKDGISMSYEEASVWGQPYFGNNFERLMEVKRKVDP 809
           MTPFVSKSPRAAY+N RDLD+GTNN DG+   Y+EAS+WG  YFGNNFERLMEVK KVDP
Sbjct: 463 MTPFVSKSPRAAYVNYRDLDMGTNNDDGVPTRYKEASLWGHRYFGNNFERLMEVKMKVDP 522

Query: 810 FNFFRHEQSIPPAPP 822
           FNFFRHEQSIPPAPP
Sbjct: 523 FNFFRHEQSIPPAPP 537

HSP 2 Score: 278.1 bits (710), Expect = 5.1e-71
Identity = 135/156 (86.54%), Postives = 144/156 (92.31%), Query Frame = 1

Query: 1   MKYFWSCYGYCFISLLLLFSLATLGCGDDNLEDFVHCVYLHSNNNNDSNSTLIHIPSSSS 60
           MKYFW CY Y FISLLLL SLATLGCG +N+EDFV C+Y+HS+NNNDSNSTLIH PSSSS
Sbjct: 1   MKYFWKCYCYSFISLLLL-SLATLGCGSNNMEDFVDCIYVHSSNNNDSNSTLIHTPSSSS 60

Query: 61  YSHVLNFSIRNRRFSEAETPKPVAIITPSHAFQVQATVICCKSHGLQIRTRSGGHDFEGR 120
           YS+VLNFSIRN RFSE ETPKPVAIITPSHA QVQATVICCKSHGLQIRTRSGGHDFEGR
Sbjct: 61  YSYVLNFSIRNLRFSEPETPKPVAIITPSHASQVQATVICCKSHGLQIRTRSGGHDFEGR 120

Query: 121 SYVANVPFVLVDLINFNSITIDTEDKSAWVQSGATV 157
           SYVANVPFVL+DLIN NSITID ED++AWVQSGATV
Sbjct: 121 SYVANVPFVLIDLINLNSITIDVEDETAWVQSGATV 155

HSP 3 Score: 265.0 bits (676), Expect = 4.5e-67
Identity = 130/150 (86.67%), Postives = 139/150 (92.67%), Query Frame = 1

Query: 432 CYGYCFISLLLLFSLATLGCGDDNLEDFVHCVYLHSNNNNDSNSTLIHTPSSSSYSHVLN 491
           CY Y FISLLLL SLATLGCG +N+EDFV C+Y+HS+NNNDSNSTLIHTPSSSSYS+VLN
Sbjct: 7   CYCYSFISLLLL-SLATLGCGSNNMEDFVDCIYVHSSNNNDSNSTLIHTPSSSSYSYVLN 66

Query: 492 FSIRNRRFSETETPKPVAIITPSHASQVQATVICCKSHGLQIRTRSGGHDLEGRSYVANV 551
           FSIRN RFSE ETPKPVAIITPSHASQVQATVICCKSHGLQIRTRSGGHD EGRSYVANV
Sbjct: 67  FSIRNLRFSEPETPKPVAIITPSHASQVQATVICCKSHGLQIRTRSGGHDFEGRSYVANV 126

Query: 552 PFVLVDLINFNLITIDTEDKSAWVQSGATV 582
           PFVL+DLIN N ITID ED++AWVQSGATV
Sbjct: 127 PFVLIDLINLNSITIDVEDETAWVQSGATV 155

HSP 4 Score: 421.4 bits (1082), Expect = 3.8e-114
Identity = 199/254 (78.35%), Postives = 227/254 (89.37%), Query Frame = 1

Query: 145 DKSAWVQSGATVASNSSNGSGRTIEASFFSLFLGNASELLALMEKIFPELGLKKEDCLEM 204
           D+  ++ +  T +++S +GSGR +EASFFSLFLGNA+ELL+LMEK FPELGLKKEDCLE 
Sbjct: 254 DQDLFISAWVTASNSSHDGSGRIMEASFFSLFLGNATELLSLMEKTFPELGLKKEDCLET 313

Query: 205 SWVEAMAFSASGFVSAQSLELLLDRIPPKIGRNKIKSDYATQPISKIALEGMWERFKIEE 264
           SWVE+MAFSASGFVSA+SLELLLDR P   GR K KSDYAT+PIS+  LEGMWERFK EE
Sbjct: 314 SWVESMAFSASGFVSAKSLELLLDRTPLHNGRYKTKSDYATEPISETVLEGMWERFKDEE 373

Query: 265 LETVQLILIPFGGKMNEISEAESPSPHRAGYPIHITYYVTWQQPDADSRHLEWTRELHDY 324
           LETVQLILIPFGGK NEISE+E+PSPHRAGYPIHI YY+TWQ+PDADS+HL+W RELH+Y
Sbjct: 374 LETVQLILIPFGGKTNEISESETPSPHRAGYPIHIGYYLTWQRPDADSKHLKWARELHNY 433

Query: 325 MTPFVSKSPRAAYINHRDFDIGTNNEDGIPTSYEEASVWGQPYFGNNFERLMEVKRKVDP 384
           MTPFVSKSPRAAY+N+RD D+GTNN+DG+PT  EEAS+WG  YFGNNFERLMEVKRKVDP
Sbjct: 434 MTPFVSKSPRAAYVNYRDLDMGTNNDDGVPTRCEEASIWGHRYFGNNFERLMEVKRKVDP 493

Query: 385 FNFFRHEQSIPPAP 399
           FNFFRHEQSIPPAP
Sbjct: 494 FNFFRHEQSIPPAP 507

BLAST of ClCG03G002520 vs. NCBI nr
Match: gi|449459146|ref|XP_004147307.1| (PREDICTED: cannabidiolic acid synthase-like [Cucumis sativus])

HSP 1 Score: 399.1 bits (1024), Expect = 2.0e-107
Identity = 194/254 (76.38%), Postives = 219/254 (86.22%), Query Frame = 1

Query: 570 DKSAWVQSGATVALNSSNGGGRTMEASFFSLFLGNASELLAHMEKIFPELGLKKEDCLEM 629
           D+  ++ +  T + +S +G GR MEASFFSLFLGNA+ELL+ MEK FPELGLKKEDCLE 
Sbjct: 254 DQDLFISAWVTASNSSHDGSGRIMEASFFSLFLGNATELLSLMEKTFPELGLKKEDCLET 313

Query: 630 SWVEAMAFSASGFVSAQSLELLLNRTPPKIGRYKTKSDYATQPISKTALEGM---FKFEE 689
           SWVE+MAFSASGFVSA+SLELLL+RTP   GRYKTKSDYAT+PIS+T LEGM   FK EE
Sbjct: 314 SWVESMAFSASGFVSAKSLELLLDRTPLHNGRYKTKSDYATEPISETVLEGMWERFKDEE 373

Query: 690 LETVRLVLIPFRGKMNEISEVEIPSPHRAGYPIHIAYYVTWQRPDADSRHLEWMQELHDY 749
           LETV+L+LIPF GK NEISE E PSPHRAGYPIHI YY+TWQRPDADS+HL+W +ELH+Y
Sbjct: 374 LETVQLILIPFGGKTNEISESETPSPHRAGYPIHIGYYLTWQRPDADSKHLKWARELHNY 433

Query: 750 MTPFVSKSPRAAYINCRDLDIGTNNKDGISMSYEEASVWGQPYFGNNFERLMEVKRKVDP 809
           MTPFVSKSPRAAY+N RDLD+GTNN DG+    EEAS+WG  YFGNNFERLMEVKRKVDP
Sbjct: 434 MTPFVSKSPRAAYVNYRDLDMGTNNDDGVPTRCEEASIWGHRYFGNNFERLMEVKRKVDP 493

Query: 810 FNFFRHEQSIPPAP 821
           FNFFRHEQSIPPAP
Sbjct: 494 FNFFRHEQSIPPAP 507

HSP 2 Score: 232.3 bits (591), Expect = 3.2e-57
Identity = 111/126 (88.10%), Postives = 120/126 (95.24%), Query Frame = 1

Query: 31  LEDFVHCVYLHSNNNNDSNSTLIHIPSSSSYSHVLNFSIRNRRFSEAETPKPVAIITPSH 90
           ++DFV+C+Y+HS+NNNDSNSTLIH PSSSSYS+VLNFSIRN RFSE ETPKPVAIITPSH
Sbjct: 1   MKDFVNCIYVHSSNNNDSNSTLIHTPSSSSYSYVLNFSIRNLRFSEPETPKPVAIITPSH 60

Query: 91  AFQVQATVICCKSHGLQIRTRSGGHDFEGRSYVANVPFVLVDLINFNSITIDTEDKSAWV 150
           A QVQATVICCKSHGLQIRTRSGGHDFEGRSYVANVPFVL+DLIN NSITID ED+SAWV
Sbjct: 61  ASQVQATVICCKSHGLQIRTRSGGHDFEGRSYVANVPFVLIDLINLNSITIDVEDESAWV 120

Query: 151 QSGATV 157
           QSGATV
Sbjct: 121 QSGATV 126

HSP 3 Score: 232.3 bits (591), Expect = 3.2e-57
Identity = 111/126 (88.10%), Postives = 120/126 (95.24%), Query Frame = 1

Query: 456 LEDFVHCVYLHSNNNNDSNSTLIHTPSSSSYSHVLNFSIRNRRFSETETPKPVAIITPSH 515
           ++DFV+C+Y+HS+NNNDSNSTLIHTPSSSSYS+VLNFSIRN RFSE ETPKPVAIITPSH
Sbjct: 1   MKDFVNCIYVHSSNNNDSNSTLIHTPSSSSYSYVLNFSIRNLRFSEPETPKPVAIITPSH 60

Query: 516 ASQVQATVICCKSHGLQIRTRSGGHDLEGRSYVANVPFVLVDLINFNLITIDTEDKSAWV 575
           ASQVQATVICCKSHGLQIRTRSGGHD EGRSYVANVPFVL+DLIN N ITID ED+SAWV
Sbjct: 61  ASQVQATVICCKSHGLQIRTRSGGHDFEGRSYVANVPFVLIDLINLNSITIDVEDESAWV 120

Query: 576 QSGATV 582
           QSGATV
Sbjct: 121 QSGATV 126

HSP 4 Score: 405.6 bits (1041), Expect = 2.1e-109
Identity = 192/231 (83.12%), Postives = 212/231 (91.77%), Query Frame = 1

Query: 168 IEASFFSLFLGNASELLALMEKIFPELGLKKEDCLEMSWVEAMAFSASGFVSAQSLELLL 227
           +EASFFSLFLGNA+ELL+LMEK FPELGLKKEDCLE SWVE+MAFSASGFVSA+SLELLL
Sbjct: 1   MEASFFSLFLGNATELLSLMEKTFPELGLKKEDCLETSWVESMAFSASGFVSAKSLELLL 60

Query: 228 DRIPPKIGRNKIKSDYATQPISKIALEGMWERFKIEELETVQLILIPFGGKMNEISEAES 287
           DR P   GR K KSDYAT+PIS+  LEGMWERFK EELETVQLILIPFGGK NEISE+E+
Sbjct: 61  DRTPLHNGRYKTKSDYATEPISETVLEGMWERFKDEELETVQLILIPFGGKTNEISESET 120

Query: 288 PSPHRAGYPIHITYYVTWQQPDADSRHLEWTRELHDYMTPFVSKSPRAAYINHRDFDIGT 347
           PSPHRAGYPIHI YY+TWQ+PDADS+HL+W RELH+YMTPFVSKSPRAAY+N+RD D+GT
Sbjct: 121 PSPHRAGYPIHIGYYLTWQRPDADSKHLKWARELHNYMTPFVSKSPRAAYVNYRDLDMGT 180

Query: 348 NNEDGIPTSYEEASVWGQPYFGNNFERLMEVKRKVDPFNFFRHEQSIPPAP 399
           NN+DG+PT  EEAS+WG  YFGNNFERLMEVKRKVDPFNFFRHEQSIPPAP
Sbjct: 181 NNDDGVPTRCEEASIWGHRYFGNNFERLMEVKRKVDPFNFFRHEQSIPPAP 231

BLAST of ClCG03G002520 vs. NCBI nr
Match: gi|700209679|gb|KGN64775.1| (hypothetical protein Csa_1G096090 [Cucumis sativus])

HSP 1 Score: 386.3 bits (991), Expect = 1.3e-103
Identity = 188/231 (81.39%), Postives = 206/231 (89.18%), Query Frame = 1

Query: 593 MEASFFSLFLGNASELLAHMEKIFPELGLKKEDCLEMSWVEAMAFSASGFVSAQSLELLL 652
           MEASFFSLFLGNA+ELL+ MEK FPELGLKKEDCLE SWVE+MAFSASGFVSA+SLELLL
Sbjct: 1   MEASFFSLFLGNATELLSLMEKTFPELGLKKEDCLETSWVESMAFSASGFVSAKSLELLL 60

Query: 653 NRTPPKIGRYKTKSDYATQPISKTALEGM---FKFEELETVRLVLIPFRGKMNEISEVEI 712
           +RTP   GRYKTKSDYAT+PIS+T LEGM   FK EELETV+L+LIPF GK NEISE E 
Sbjct: 61  DRTPLHNGRYKTKSDYATEPISETVLEGMWERFKDEELETVQLILIPFGGKTNEISESET 120

Query: 713 PSPHRAGYPIHIAYYVTWQRPDADSRHLEWMQELHDYMTPFVSKSPRAAYINCRDLDIGT 772
           PSPHRAGYPIHI YY+TWQRPDADS+HL+W +ELH+YMTPFVSKSPRAAY+N RDLD+GT
Sbjct: 121 PSPHRAGYPIHIGYYLTWQRPDADSKHLKWARELHNYMTPFVSKSPRAAYVNYRDLDMGT 180

Query: 773 NNKDGISMSYEEASVWGQPYFGNNFERLMEVKRKVDPFNFFRHEQSIPPAP 821
           NN DG+    EEAS+WG  YFGNNFERLMEVKRKVDPFNFFRHEQSIPPAP
Sbjct: 181 NNDDGVPTRCEEASIWGHRYFGNNFERLMEVKRKVDPFNFFRHEQSIPPAP 231

HSP 2 Score: 326.6 bits (836), Expect = 1.3e-85
Identity = 186/421 (44.18%), Postives = 252/421 (59.86%), Query Frame = 1

Query: 11  CFISLLLLFSLATLGCGDDNLED-FVHCVYLHSNNNNDSNSTLIHIPSSSSYSHVLNFSI 70
           CF   + L  L+        + D F+ C+ +HS  +  S + +IH P+SS YS+ L  S 
Sbjct: 3   CFTLTVFLLMLSVSSSTSATVNDHFLQCMSIHSTPHAKS-AQIIHQPNSSLYSYFLQSSQ 62

Query: 71  RNRRFSEAETPKPVAIITPSHAFQVQATVICCKSHGLQIRTRSGGHDFEGRSYVANVPFV 130
           +N R+  A T KP+ I+TP H  ++QA ++C +  GLQIRTRSGGHD+EG SY++  PF+
Sbjct: 63  QNPRWLNASTSKPLLILTPFHESEIQAAILCSRKQGLQIRTRSGGHDYEGLSYLSEAPFI 122

Query: 131 LVD----------LINFNSITIDTE---DKSAWVQSGATVASNSSNGSGR---------- 190
           +            LI+ N   ++ E   +   W   G   AS     S +          
Sbjct: 123 IYGLAADNILDAYLIDVNGRILNRESMGESLFWAIRGGGGASFGILVSWKIKLVRVPPTV 182

Query: 191 --TIEASFFSLFLGNASELLALMEKIFPELGLKKEDCLEMSWVEAMAFSASGFVSAQSLE 250
             TI+ASF SLFLG   +L+ LM K FPELGL+  DC EM+W+E++ + A GF    SL+
Sbjct: 183 TMTIQASFNSLFLGGVDKLIPLMGKSFPELGLQAGDCTEMTWIESVLYFA-GFPRGNSLD 242

Query: 251 LLLDRIPPKIGRNKIKSDYATQPISKIALEGMWERFKIEELETVQLILIPFGGKMNEISE 310
           +LL+R  P     K KSDY  +PI ++ LEG+WERF  E++  V +I+ P+GG+MN+ISE
Sbjct: 243 VLLNRTHPDRSYFKAKSDYVKEPIPEVGLEGVWERFLKEQI--VFMIMDPYGGRMNKISE 302

Query: 311 AESPSPHRAGYPIHITYYVTWQQPDA--DSRHLEWTRELHDYMTPFVSKSPRAAYINHRD 370
           +E P PHR G   +I Y V W+  +A   ++H+ W R L  YM PFVSKSPRAAY+N+RD
Sbjct: 303 SELPFPHRKGNLYNIQYLVKWEVNEAKISNKHVHWIRMLFQYMRPFVSKSPRAAYLNYRD 362

Query: 371 FDIGTNNEDGIPTSYEEASVWGQPYFGNNFERLMEVKRKVDPFNFFRHEQSIPPAPPREA 404
            D+G NN+D    SY +A VWG  YF  NF RL  VK KVDP NFFR+EQSIPP P  E 
Sbjct: 363 LDLGINNQDN--ASYSQAWVWGTKYFKGNFFRLAWVKAKVDPDNFFRNEQSIPPLPHMEN 417

BLAST of ClCG03G002520 vs. NCBI nr
Match: gi|297740877|emb|CBI31059.3| (unnamed protein product [Vitis vinifera])

HSP 1 Score: 314.7 bits (805), Expect = 4.9e-82
Identity = 177/414 (42.75%), Postives = 247/414 (59.66%), Query Frame = 1

Query: 436 CFISLLLLFSLATLGCGDDNLED-FVHCVYLHSNNNNDSNSTLIHTPSSSSYSHVLNFSI 495
           CF   + L  L+        + D F+ C+ +HS  +  S + +IH P+SS YS+ L  S 
Sbjct: 3   CFTLTVFLLMLSVSSSTSATVNDHFLQCMSIHSTPHAKS-AQIIHQPNSSLYSYFLQSSQ 62

Query: 496 RNRRFSETETPKPVAIITPSHASQVQATVICCKSHGLQIRTRSGGHDLEGRSYVANVPFV 555
           +N R+    T KP+ I+TP H S++QA ++C +  GLQIRTRSGGHD EG SY++  PF+
Sbjct: 63  QNPRWLNASTSKPLLILTPFHESEIQAAILCSRKQGLQIRTRSGGHDYEGLSYLSEAPFI 122

Query: 556 LVDLINFNLI---TIDTEDKSAWVQSGATVALNSSNGGG--------------------- 615
           +  L   N++    ID   +    +S       +  GGG                     
Sbjct: 123 IYGLAADNILDAYLIDVNGRILNRESMGESLFWAIRGGGGASFGILVSWKIKLVRVPPTV 182

Query: 616 -RTMEASFFSLFLGNASELLAHMEKIFPELGLKKEDCLEMSWVEAMAFSASGFVSAQSLE 675
             T++ASF SLFLG   +L+  M K FPELGL+  DC EM+W+E++ + A GF    SL+
Sbjct: 183 TMTIQASFNSLFLGGVDKLIPLMGKSFPELGLQAGDCTEMTWIESVLYFA-GFPRGNSLD 242

Query: 676 LLLNRTPPKIGRYKTKSDYATQPISKTALEGMF-KFEELETVRLVLIPFRGKMNEISEVE 735
           +LLNRT P    +K KSDY  +PI +  LEG++ +F + + V +++ P+ G+MN+ISE E
Sbjct: 243 VLLNRTHPDRSYFKAKSDYVKEPIPEVGLEGVWERFLKEQIVFMIMDPYGGRMNKISESE 302

Query: 736 IPSPHRAGYPIHIAYYVTWQRPDA--DSRHLEWMQELHDYMTPFVSKSPRAAYINCRDLD 795
           +P PHR G   +I Y V W+  +A   ++H+ W++ L  YM PFVSKSPRAAY+N RDLD
Sbjct: 303 LPFPHRKGNLYNIQYLVKWEVNEAKISNKHVHWIRMLFQYMRPFVSKSPRAAYLNYRDLD 362

Query: 796 IGTNNKDGISMSYEEASVWGQPYFGNNFERLMEVKRKVDPFNFFRHEQSIPPAP 821
           +G NN+D  + SY +A VWG  YF  NF RL  VK KVDP NFFR+EQSIPP P
Sbjct: 363 LGINNQD--NASYSQAWVWGTKYFKGNFFRLAWVKAKVDPDNFFRNEQSIPPLP 412

HSP 2 Score: 306.2 bits (783), Expect = 1.8e-79
Identity = 178/412 (43.20%), Postives = 246/412 (59.71%), Query Frame = 1

Query: 10  YCFISLLLLFSLATLGCGDDNLEDFVHCVYLHSNNNNDSNSTLIHIPSSSSYSHVLNFSI 69
           + F   L  FS  T      + EDF+ C+ L S +++ S S +I+ P +SSY  VL   I
Sbjct: 9   FLFAVFLFSFSWVTTTSAHTH-EDFLECLKLQSEDSS-SISKVIYTPINSSYLSVLQLGI 68

Query: 70  RNRRFSEAETPKPVAIITPSHAFQVQATVICCKSHGLQIRTRSGGHDFEGRSYVANVPFV 129
            N RF+ + TPKP  I+TP +   ++AT+ C K H LQIR RSGGHD+EG SYV+  PFV
Sbjct: 69  NNERFNTSTTPKPQVIVTPLNLSHIEATITCSKKHDLQIRIRSGGHDYEGLSYVSPNPFV 128

Query: 130 LVDLINFNSITIDTEDKSAWVQSGAT-------VASNSSN-----------GSGRTIEAS 189
           ++DLI   SIT+D  +K+AWVQ GAT       +A  S N           G G      
Sbjct: 129 ILDLITLQSITVDATNKTAWVQVGATTGKLYYSIAEKSQNLAFPASVCTTVGIGGHFSGG 188

Query: 190 FFSLFL---GNASELLALMEKIFPELGLKKEDCLEMSWVEAMAFSASGFVSAQSLELLLD 249
            +   L   G A++ + +  ++    GL KED  EMSW+E++ + A GF S  SLE+LL+
Sbjct: 189 GYGTLLRKYGLAADNI-IDAQLTDVDGLTKEDSTEMSWIESVLYFA-GF-SNSSLEILLE 248

Query: 250 RIPPKIGRNKIKSDYATQPISKIALEGMWERFKIEELETVQLILIPFGGKMNEISEAESP 309
           R    +   K KSDY  QP+ ++ALEG+W+R    E+ + QL+   +GG+++EISE+  P
Sbjct: 249 RTQQNVRYFKAKSDYVQQPMPEVALEGIWKRLFEVEVGSGQLMFNLYGGRVSEISESSIP 308

Query: 310 SPHRAGYPIHITYYVTWQQPDADS--RHLEWTRELHDYMTPFVSKSPRAAYINHRDFDIG 369
            PHRAG    I + V W     +    H+ W R L+ ++ P+VSK+PR+AYIN+RD DIG
Sbjct: 309 FPHRAGNLYKIQHSVYWNDEGIEESITHISWIRRLYSFLAPYVSKNPRSAYINYRDLDIG 368

Query: 370 TNNEDGIPTSYEEASVWGQPYFGNNFERLMEVKRKVDPFNFFRHEQSIPPAP 399
            NN     TSYE+AS+WG  YF  NF+RL+ VK +VDP NFFR+EQSIP  P
Sbjct: 369 INNIGN--TSYEQASIWGIKYFKINFDRLVHVKTQVDPANFFRNEQSIPSLP 413