ClCG02G005620 (gene) Watermelon (Charleston Gray) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	ClCG02G005620
Type	gene
Organism	Citrullus lanatus (Watermelon (Charleston Gray))
Description	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Location	CG_Chr02 : 6130425 .. 6130839 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACAGATGCAACTAAGTAAATTTGGTATTAAAATGGTGTACTTTAACCATTCCCATACACTCTATATTTATTTATAAGAGAGTTCTTTGGTGCAACCAAAACTTCACATTCTCCATATCTCCATCCACATTCATTTGCCCCCTCAAAATTTCCATCCGCCATGGGAGACCTTGAGGAGTTCAATCAGGCTTTCATCCAAGACCCCAAGTTCGCCATCTCCAAAGCCGACGGAATCCCCACCATCGACCTCTCTCCGATCTTCAACCCACCTGAGCGGTGATTTCTCCGACGATGTGGTTCGACAAATCGCCTCTGCTTCCAAGGAGTGGGGATTCTTTTTGGTGGTAAACCACGGCGTCCCTCTCGAGAAGCGGCGCCGGATAGAGGCCGCTGCCAGGGAGTTTTTCGGGTAG

mRNA sequence

ATGACAGATGCAACTAATTCTTTGGTGCAACCAAAACTTCACATTCTCCATATCTCCATCCACATTCATTTGCCCCCTCAAAATTTCCATCCGCCATGGGAGACCTTGAGGAGTTCAATCAGGCTTTCATCCAAGACCCCAAGTTCGCCATCTCCAAAGCCGACGGAATCCCCACCATCGACCTCTCTCCGATCTTCAACCCACCTGAGCGGTGATTTCTCCGACGATGTGGTTCGACAAATCGCCTCTGCTTCCAAGGAGTGGGGATTCTTTTTGGTGGTAAACCACGGCGTCCCTCTCGAGAAGCGGCGCCGGATAGAGGCCGCTGCCAGGGAGTTTTTCGGGTAG

Coding sequence (CDS)

ATGACAGATGCAACTAATTCTTTGGTGCAACCAAAACTTCACATTCTCCATATCTCCATCCACATTCATTTGCCCCCTCAAAATTTCCATCCGCCATGGGAGACCTTGAGGAGTTCAATCAGGCTTTCATCCAAGACCCCAAGTTCGCCATCTCCAAAGCCGACGGAATCCCCACCATCGACCTCTCTCCGATCTTCAACCCACCTGAGCGGTGATTTCTCCGACGATGTGGTTCGACAAATCGCCTCTGCTTCCAAGGAGTGGGGATTCTTTTTGGTGGTAAACCACGGCGTCCCTCTCGAGAAGCGGCGCCGGATAGAGGCCGCTGCCAGGGAGTTTTTCGGGTAG

Protein sequence

MTDATNSLVQPKLHILHISIHIHLPPQNFHPPWETLRSSIRLSSKTPSSPSPKPTESPPSTSLRSSTHLSGDFSDDVVRQIASASKEWGFFLVVNHGVPLEKRRRIEAAAREFFG

Homology

[Close]

BLAST of ClCG02G005620 vs. Swiss-Prot
Match: FLS1_ARATH (Flavonol synthase/flavanone 3-hydroxylase OS=Arabidopsis thaliana GN=FLS1 PE=1 SV=1)

HSP 1 Score: 52.8 bits (125), Expect = 3.0e-06
Identity = 31/87 (35.63%), Postives = 40/87 (45.98%), Query Frame = 1


Query: 34  ETLRSSIRLSSKTPSSPSPKPTESPPSTSLRSST------HLSGDFSDDVVRQIASASKE 93
           + + SS  L+   P        E P  T+ R  T       LS    + V R +  AS+E
Sbjct: 7   QDISSSSLLTEAIPLEFIRSEKEQPAITTFRGPTPAIPVVDLSDPDEESVRRAVVKASEE 66

Query: 94  WGFFLVVNHGVPLEKRRRIEAAAREFF 115
           WG F VVNHG+P E  RR++   R+FF
Sbjct: 67  WGLFQVVNHGIPTELIRRLQDVGRKFF 93

[Close]

BLAST of ClCG02G005620 vs. TrEMBL
Match: K7ZTB8_CUCSA (Gibberellin 20-oxidase (Fragment) OS=Cucumis sativus GN=ga20ox2 PE=2 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 1.3e-11
Identity = 41/65 (63.08%), Postives = 47/65 (72.31%), Query Frame = 1


Query: 51  SPKPTESPPSTSLRSSTHLSGDFSDDVVRQIASASKEWGFFLVVNHGVPLEKRRRIEAAA 110
           SP   +SPP+ S         +F  D+V+QIASA  EWGFFLVVNHGVP EKRRR+EAAA
Sbjct: 35  SPIFNDSPPAGS---------EFPHDLVQQIASACTEWGFFLVVNHGVPPEKRRRMEAAA 90

Query: 111 REFFG 116
           REFFG
Sbjct: 95  REFFG 90

[Close]

BLAST of ClCG02G005620 vs. TrEMBL
Match: A0A0A0K6P5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G074950 PE=3 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 1.3e-11
Identity = 41/65 (63.08%), Postives = 47/65 (72.31%), Query Frame = 1


Query: 51  SPKPTESPPSTSLRSSTHLSGDFSDDVVRQIASASKEWGFFLVVNHGVPLEKRRRIEAAA 110
           SP   +SPP+ S         +F  D+V+QIASA  EWGFFLVVNHGVP EKRRR+EAAA
Sbjct: 35  SPIFNDSPPAGS---------EFPHDLVQQIASACTEWGFFLVVNHGVPPEKRRRMEAAA 90

Query: 111 REFFG 116
           REFFG
Sbjct: 95  REFFG 90

[Close]

BLAST of ClCG02G005620 vs. TrEMBL
Match: A0A0A0K3A9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G074960 PE=3 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 6.2e-11
Identity = 36/44 (81.82%), Postives = 37/44 (84.09%), Query Frame = 1


Query: 72  DFSDDVVRQIASASKEWGFFLVVNHGVPLEKRRRIEAAAREFFG 116
           DF  D+V QIASA  EWGFFLVVNHGVP EKR RIEAAAREFFG
Sbjct: 41  DFPHDLVHQIASACTEWGFFLVVNHGVPPEKRHRIEAAAREFFG 84

[Close]

BLAST of ClCG02G005620 vs. TrEMBL
Match: A0A0A0K834_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G074940 PE=4 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 4.0e-10
Identity = 35/44 (79.55%), Postives = 36/44 (81.82%), Query Frame = 1


Query: 72  DFSDDVVRQIASASKEWGFFLVVNHGVPLEKRRRIEAAAREFFG 116
           D   D+V QIASA  EWGFFLVVNHGVP EKR RIEAAAREFFG
Sbjct: 46  DVPHDLVHQIASACTEWGFFLVVNHGVPPEKRHRIEAAAREFFG 89

[Close]

BLAST of ClCG02G005620 vs. TrEMBL
Match: W9R171_9ROSA (Naringenin,2-oxoglutarate 3-dioxygenase OS=Morus notabilis GN=L484_027018 PE=3 SV=1)

HSP 1 Score: 71.2 bits (173), Expect = 9.0e-10
Identity = 35/47 (74.47%), Postives = 39/47 (82.98%), Query Frame = 1


Query: 69  LSGDFSDD-VVRQIASASKEWGFFLVVNHGVPLEKRRRIEAAAREFF 115
           LSG  +DD +VR+I  A K+WGFF VVNHGVP EKRRRIEAAAREFF
Sbjct: 33  LSGGLTDDELVREIGKACKQWGFFQVVNHGVPAEKRRRIEAAAREFF 79

[Close]

BLAST of ClCG02G005620 vs. TAIR10
Match: AT5G08640.1 (AT5G08640.1 flavonol synthase 1)

HSP 1 Score: 52.8 bits (125), Expect = 1.7e-07
Identity = 31/87 (35.63%), Postives = 40/87 (45.98%), Query Frame = 1


Query: 34  ETLRSSIRLSSKTPSSPSPKPTESPPSTSLRSST------HLSGDFSDDVVRQIASASKE 93
           + + SS  L+   P        E P  T+ R  T       LS    + V R +  AS+E
Sbjct: 7   QDISSSSLLTEAIPLEFIRSEKEQPAITTFRGPTPAIPVVDLSDPDEESVRRAVVKASEE 66

Query: 94  WGFFLVVNHGVPLEKRRRIEAAAREFF 115
           WG F VVNHG+P E  RR++   R+FF
Sbjct: 67  WGLFQVVNHGIPTELIRRLQDVGRKFF 93

[Close]

BLAST of ClCG02G005620 vs. TAIR10
Match: AT3G19000.1 (AT3G19000.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 50.1 bits (118), Expect = 1.1e-06
Identity = 30/73 (41.10%), Postives = 36/73 (49.32%), Query Frame = 1


Query: 64  RSSTHL--SGDF--SDDV------------------VRQIASASKEWGFFLVVNHGVPLE 115
           R +THL  SGDF  SD++                   ++IA A K WGFF V+NHG+P  
Sbjct: 15  RPNTHLTNSGDFIFSDEIPTIDLSSLEDTHHDKTAIAKEIAEACKRWGFFQVINHGLPSA 74

[Close]

BLAST of ClCG02G005620 vs. TAIR10
Match: AT5G63600.2 (AT5G63600.2 flavonol synthase 5)

HSP 1 Score: 48.9 bits (115), Expect = 2.4e-06
Identity = 21/46 (45.65%), Postives = 28/46 (60.87%), Query Frame = 1


Query: 69  LSGDFSDDVVRQIASASKEWGFFLVVNHGVPLEKRRRIEAAAREFF 115
           LS    D +VR++  AS+EWG F VVNHG+P E  R+++    +FF
Sbjct: 38  LSVSDEDFLVREVVKASEEWGVFQVVNHGIPTELMRQLQMVGTQFF 83

[Close]

BLAST of ClCG02G005620 vs. TAIR10
Match: AT5G63595.1 (AT5G63595.1 flavonol synthase 4)

HSP 1 Score: 48.9 bits (115), Expect = 2.4e-06
Identity = 24/56 (42.86%), Postives = 30/56 (53.57%), Query Frame = 1


Query: 59  PSTSLRSSTHLSGDFSDDVVRQIASASKEWGFFLVVNHGVPLEKRRRIEAAAREFF 115
           PS+       LS    + V R++A AS+EWG F VVNHG+P E  RR+     +FF
Sbjct: 20  PSSQNFPVVDLSNTNGELVARKVAKASEEWGIFQVVNHGIPTELIRRLHKVDTQFF 75

[Close]

BLAST of ClCG02G005620 vs. TAIR10
Match: AT5G43935.1 (AT5G43935.1 flavonol synthase 6)

HSP 1 Score: 47.8 bits (112), Expect = 5.4e-06
Identity = 21/44 (47.73%), Postives = 28/44 (63.64%), Query Frame = 1


Query: 72  DFSDDVVRQ-IASASKEWGFFLVVNHGVPLEKRRRIEAAAREFF 115
           D SD++V   +  AS+EWG F +VNHG+P E  RR++   R+FF
Sbjct: 26  DPSDELVAHAVVKASEEWGIFQLVNHGIPAELMRRLQEVGRQFF 69

[Close]

BLAST of ClCG02G005620 vs. NCBI nr
Match: gi|413915332|emb|CBX88044.1| (gibberellin 20-oxidase, partial [Cucumis sativus])

HSP 1 Score: 77.4 bits (189), Expect = 1.8e-11
Identity = 41/65 (63.08%), Postives = 47/65 (72.31%), Query Frame = 1


Query: 51  SPKPTESPPSTSLRSSTHLSGDFSDDVVRQIASASKEWGFFLVVNHGVPLEKRRRIEAAA 110
           SP   +SPP+ S         +F  D+V+QIASA  EWGFFLVVNHGVP EKRRR+EAAA
Sbjct: 35  SPIFNDSPPAGS---------EFPHDLVQQIASACTEWGFFLVVNHGVPPEKRRRMEAAA 90

Query: 111 REFFG 116
           REFFG
Sbjct: 95  REFFG 90

[Close]

BLAST of ClCG02G005620 vs. NCBI nr
Match: gi|449438440|ref|XP_004136996.1| (PREDICTED: hyoscyamine 6-dioxygenase-like isoform X2 [Cucumis sativus])

HSP 1 Score: 77.4 bits (189), Expect = 1.8e-11
Identity = 41/65 (63.08%), Postives = 47/65 (72.31%), Query Frame = 1


Query: 51  SPKPTESPPSTSLRSSTHLSGDFSDDVVRQIASASKEWGFFLVVNHGVPLEKRRRIEAAA 110
           SP   +SPP+ S         +F  D+V+QIASA  EWGFFLVVNHGVP EKRRR+EAAA
Sbjct: 35  SPIFNDSPPAGS---------EFPHDLVQQIASACTEWGFFLVVNHGVPPEKRRRMEAAA 90

Query: 111 REFFG 116
           REFFG
Sbjct: 95  REFFG 90

[Close]

BLAST of ClCG02G005620 vs. NCBI nr
Match: gi|659109829|ref|XP_008454903.1| (PREDICTED: LOW QUALITY PROTEIN: gibberellin 20 oxidase 1-like [Cucumis melo])

HSP 1 Score: 77.0 bits (188), Expect = 2.4e-11
Identity = 36/44 (81.82%), Postives = 39/44 (88.64%), Query Frame = 1


Query: 72  DFSDDVVRQIASASKEWGFFLVVNHGVPLEKRRRIEAAAREFFG 116
           DF DD+V+QIA+A  EWGFFLVVNHGVP EKR RIEAAAREFFG
Sbjct: 45  DFPDDLVQQIAAACTEWGFFLVVNHGVPPEKRHRIEAAAREFFG 88

[Close]

BLAST of ClCG02G005620 vs. NCBI nr
Match: gi|778725230|ref|XP_011658920.1| (PREDICTED: gibberellin 20 oxidase 2-like isoform X3 [Cucumis sativus])

HSP 1 Score: 75.1 bits (183), Expect = 8.9e-11
Identity = 36/44 (81.82%), Postives = 37/44 (84.09%), Query Frame = 1


Query: 72  DFSDDVVRQIASASKEWGFFLVVNHGVPLEKRRRIEAAAREFFG 116
           DF  D+V QIASA  EWGFFLVVNHGVP EKR RIEAAAREFFG
Sbjct: 41  DFPHDLVHQIASACTEWGFFLVVNHGVPPEKRHRIEAAAREFFG 84

[Close]

BLAST of ClCG02G005620 vs. NCBI nr
Match: gi|778725233|ref|XP_004136997.2| (PREDICTED: gibberellin 20 oxidase 1-like isoform X4 [Cucumis sativus])

HSP 1 Score: 75.1 bits (183), Expect = 8.9e-11
Identity = 36/44 (81.82%), Postives = 37/44 (84.09%), Query Frame = 1


Query: 72  DFSDDVVRQIASASKEWGFFLVVNHGVPLEKRRRIEAAAREFFG 116
           DF  D+V QIASA  EWGFFLVVNHGVP EKR RIEAAAREFFG
Sbjct: 41  DFPHDLVHQIASACTEWGFFLVVNHGVPPEKRHRIEAAAREFFG 84

The following BLAST results are available for this feature:

BLAST of ClCG02G005620 vs. Swiss-Prot


Match Name	E-value	Identity	Description
FLS1_ARATH	3.0e-06	35.63	Flavonol synthase/flavanone 3-hydroxylase OS=Arabidopsis thaliana GN=FLS1 PE=1 S...	[more]

BLAST of ClCG02G005620 vs. TrEMBL


Match Name	E-value	Identity	Description
K7ZTB8_CUCSA	1.3e-11	63.08	Gibberellin 20-oxidase (Fragment) OS=Cucumis sativus GN=ga20ox2 PE=2 SV=1	[more]
A0A0A0K6P5_CUCSA	1.3e-11	63.08	Uncharacterized protein OS=Cucumis sativus GN=Csa_7G074950 PE=3 SV=1	[more]
A0A0A0K3A9_CUCSA	6.2e-11	81.82	Uncharacterized protein OS=Cucumis sativus GN=Csa_7G074960 PE=3 SV=1	[more]
A0A0A0K834_CUCSA	4.0e-10	79.55	Uncharacterized protein OS=Cucumis sativus GN=Csa_7G074940 PE=4 SV=1	[more]
W9R171_9ROSA	9.0e-10	74.47	Naringenin,2-oxoglutarate 3-dioxygenase OS=Morus notabilis GN=L484_027018 PE=3 S...	[more]

BLAST of ClCG02G005620 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G08640.1	1.7e-07	35.63	flavonol synthase 1	[more]
AT3G19000.1	1.1e-06	41.10	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot...	[more]
AT5G63600.2	2.4e-06	45.65	flavonol synthase 5	[more]
AT5G63595.1	2.4e-06	42.86	flavonol synthase 4	[more]
AT5G43935.1	5.4e-06	47.73	flavonol synthase 6	[more]

BLAST of ClCG02G005620 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|413915332\|emb\|CBX88044.1\|	1.8e-11	63.08	gibberellin 20-oxidase, partial [Cucumis sativus]	[more]
gi\|449438440\|ref\|XP_004136996.1\|	1.8e-11	63.08	PREDICTED: hyoscyamine 6-dioxygenase-like isoform X2 [Cucumis sativus]	[more]
gi\|659109829\|ref\|XP_008454903.1\|	2.4e-11	81.82	PREDICTED: LOW QUALITY PROTEIN: gibberellin 20 oxidase 1-like [Cucumis melo]	[more]
gi\|778725230\|ref\|XP_011658920.1\|	8.9e-11	81.82	PREDICTED: gibberellin 20 oxidase 2-like isoform X3 [Cucumis sativus]	[more]
gi\|778725233\|ref\|XP_004136997.2\|	8.9e-11	81.82	PREDICTED: gibberellin 20 oxidase 1-like isoform X4 [Cucumis sativus]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR026992	DIOX_N
IPR027443	IPNS-like

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
ClCG02G005620.1	ClCG02G005620.1	mRNA

InterPro

Analysis Name: InterPro Annotations of watermelon (Charleston Gray)
Date Performed: 2016-09-28


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 72..114 score: 3.6
IPR027443	Isopenicillin N synthase-like	GENE3D	G3DSA:2.60.120.330		coord: 46..114 score: 3.2
None	No IPR available	PANTHER	PTHR10209	OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEIN	coord: 75..114 score: 5.1
None	No IPR available	PANTHER	PTHR10209:SF98	SUBFAMILY NOT NAMED	coord: 75..114 score: 5.1
None	No IPR available	unknown	SSF51197	Clavaminate synthase-like	coord: 45..114 score: 6.87

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
ClCG02G005620	Cla97C02G032590	Watermelon (97103) v2	wcgwmbB138

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
ClCG02G005620	Silver-seed gourd	carwcgB0203
ClCG02G005620	Silver-seed gourd	carwcgB0786
ClCG02G005620	Silver-seed gourd	carwcgB0868
ClCG02G005620	Cucumber (Chinese Long) v3	cucwcgB140
ClCG02G005620	Cucumber (Chinese Long) v3	cucwcgB572
ClCG02G005620	Watermelon (97103) v2	wcgwmbB129
ClCG02G005620	Watermelon (97103) v2	wcgwmbB171
ClCG02G005620	Wax gourd	wcgwgoB275
ClCG02G005620	Wax gourd	wcgwgoB314
ClCG02G005620	Watermelon (Charleston Gray)	wcgwcgB017
ClCG02G005620	Watermelon (Charleston Gray)	wcgwcgB086
ClCG02G005620	Watermelon (Charleston Gray)	wcgwcgB138
ClCG02G005620	Cucumber (Gy14) v1	cgywcgB156
ClCG02G005620	Cucumber (Gy14) v1	cgywcgB396
ClCG02G005620	Cucumber (Gy14) v1	cgywcgB405
ClCG02G005620	Cucurbita maxima (Rimu)	cmawcgB101
ClCG02G005620	Cucurbita maxima (Rimu)	cmawcgB460
ClCG02G005620	Cucurbita maxima (Rimu)	cmawcgB485
ClCG02G005620	Cucurbita maxima (Rimu)	cmawcgB531
ClCG02G005620	Cucurbita maxima (Rimu)	cmawcgB757
ClCG02G005620	Cucurbita moschata (Rifu)	cmowcgB088
ClCG02G005620	Cucurbita moschata (Rifu)	cmowcgB459
ClCG02G005620	Cucurbita moschata (Rifu)	cmowcgB485
ClCG02G005620	Cucurbita moschata (Rifu)	cmowcgB759
ClCG02G005620	Wild cucumber (PI 183967)	cpiwcgB131
ClCG02G005620	Wild cucumber (PI 183967)	cpiwcgB136
ClCG02G005620	Wild cucumber (PI 183967)	cpiwcgB576
ClCG02G005620	Cucumber (Chinese Long) v2	cuwcgB127
ClCG02G005620	Cucumber (Chinese Long) v2	cuwcgB547
ClCG02G005620	Melon (DHL92) v3.5.1	mewcgB104
ClCG02G005620	Melon (DHL92) v3.5.1	mewcgB197
ClCG02G005620	Melon (DHL92) v3.5.1	mewcgB518
ClCG02G005620	Watermelon (97103) v1	wcgwmB192
ClCG02G005620	Watermelon (97103) v1	wcgwmB198
ClCG02G005620	Cucurbita pepo (Zucchini)	cpewcgB233
ClCG02G005620	Cucurbita pepo (Zucchini)	cpewcgB267
ClCG02G005620	Cucurbita pepo (Zucchini)	cpewcgB428
ClCG02G005620	Cucurbita pepo (Zucchini)	cpewcgB590
ClCG02G005620	Cucurbita pepo (Zucchini)	cpewcgB662
ClCG02G005620	Bottle gourd (USVL1VR-Ls)	lsiwcgB057
ClCG02G005620	Bottle gourd (USVL1VR-Ls)	lsiwcgB097
ClCG02G005620	Bottle gourd (USVL1VR-Ls)	lsiwcgB132
ClCG02G005620	Bottle gourd (USVL1VR-Ls)	lsiwcgB428
ClCG02G005620	Cucumber (Gy14) v2	cgybwcgB122
ClCG02G005620	Cucumber (Gy14) v2	cgybwcgB125
ClCG02G005620	Cucumber (Gy14) v2	cgybwcgB507
ClCG02G005620	Melon (DHL92) v3.6.1	medwcgB192
ClCG02G005620	Melon (DHL92) v3.6.1	medwcgB272
ClCG02G005620	Melon (DHL92) v3.6.1	medwcgB513