ClCG02G005620.1 (mRNA) Watermelon (Charleston Gray) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	ClCG02G005620.1
Type	mRNA
Organism	Citrullus lanatus (Watermelon (Charleston Gray))
Description	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Location	CG_Chr02 : 6130425 .. 6130839 (+)
Sequence length	348

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACAGATGCAACTAAGTAAATTTGGTATTAAAATGGTGTACTTTAACCATTCCCATACACTCTATATTTATTTATAAGAGAGTTCTTTGGTGCAACCAAAACTTCACATTCTCCATATCTCCATCCACATTCATTTGCCCCCTCAAAATTTCCATCCGCCATGGGAGACCTTGAGGAGTTCAATCAGGCTTTCATCCAAGACCCCAAGTTCGCCATCTCCAAAGCCGACGGAATCCCCACCATCGACCTCTCTCCGATCTTCAACCCACCTGAGCGGTGATTTCTCCGACGATGTGGTTCGACAAATCGCCTCTGCTTCCAAGGAGTGGGGATTCTTTTTGGTGGTAAACCACGGCGTCCCTCTCGAGAAGCGGCGCCGGATAGAGGCCGCTGCCAGGGAGTTTTTCGGGTAG

mRNA sequence

ATGACAGATGCAACTAATTCTTTGGTGCAACCAAAACTTCACATTCTCCATATCTCCATCCACATTCATTTGCCCCCTCAAAATTTCCATCCGCCATGGGAGACCTTGAGGAGTTCAATCAGGCTTTCATCCAAGACCCCAAGTTCGCCATCTCCAAAGCCGACGGAATCCCCACCATCGACCTCTCTCCGATCTTCAACCCACCTGAGCGGTGATTTCTCCGACGATGTGGTTCGACAAATCGCCTCTGCTTCCAAGGAGTGGGGATTCTTTTTGGTGGTAAACCACGGCGTCCCTCTCGAGAAGCGGCGCCGGATAGAGGCCGCTGCCAGGGAGTTTTTCGGGTAG

Coding sequence (CDS)

ATGACAGATGCAACTAATTCTTTGGTGCAACCAAAACTTCACATTCTCCATATCTCCATCCACATTCATTTGCCCCCTCAAAATTTCCATCCGCCATGGGAGACCTTGAGGAGTTCAATCAGGCTTTCATCCAAGACCCCAAGTTCGCCATCTCCAAAGCCGACGGAATCCCCACCATCGACCTCTCTCCGATCTTCAACCCACCTGAGCGGTGATTTCTCCGACGATGTGGTTCGACAAATCGCCTCTGCTTCCAAGGAGTGGGGATTCTTTTTGGTGGTAAACCACGGCGTCCCTCTCGAGAAGCGGCGCCGGATAGAGGCCGCTGCCAGGGAGTTTTTCGGGTAG

Protein sequence

MTDATNSLVQPKLHILHISIHIHLPPQNFHPPWETLRSSIRLSSKTPSSPSPKPTESPPSTSLRSSTHLSGDFSDDVVRQIASASKEWGFFLVVNHGVPLEKRRRIEAAAREFFG

Homology

[Close]

BLAST of ClCG02G005620.1 vs. Swiss-Prot
Match: FLS1_ARATH (Flavonol synthase/flavanone 3-hydroxylase OS=Arabidopsis thaliana GN=FLS1 PE=1 SV=1)

HSP 1 Score: 52.8 bits (125), Expect = 3.0e-06
Identity = 31/87 (35.63%), Postives = 40/87 (45.98%), Query Frame = 1


Query: 34  ETLRSSIRLSSKTPSSPSPKPTESPPSTSLRSST------HLSGDFSDDVVRQIASASKE 93
           + + SS  L+   P        E P  T+ R  T       LS    + V R +  AS+E
Sbjct: 7   QDISSSSLLTEAIPLEFIRSEKEQPAITTFRGPTPAIPVVDLSDPDEESVRRAVVKASEE 66

Query: 94  WGFFLVVNHGVPLEKRRRIEAAAREFF 115
           WG F VVNHG+P E  RR++   R+FF
Sbjct: 67  WGLFQVVNHGIPTELIRRLQDVGRKFF 93

[Close]

BLAST of ClCG02G005620.1 vs. TrEMBL
Match: K7ZTB8_CUCSA (Gibberellin 20-oxidase (Fragment) OS=Cucumis sativus GN=ga20ox2 PE=2 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 1.3e-11
Identity = 41/65 (63.08%), Postives = 47/65 (72.31%), Query Frame = 1


Query: 51  SPKPTESPPSTSLRSSTHLSGDFSDDVVRQIASASKEWGFFLVVNHGVPLEKRRRIEAAA 110
           SP   +SPP+ S         +F  D+V+QIASA  EWGFFLVVNHGVP EKRRR+EAAA
Sbjct: 35  SPIFNDSPPAGS---------EFPHDLVQQIASACTEWGFFLVVNHGVPPEKRRRMEAAA 90

Query: 111 REFFG 116
           REFFG
Sbjct: 95  REFFG 90

[Close]

BLAST of ClCG02G005620.1 vs. TrEMBL
Match: A0A0A0K6P5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G074950 PE=3 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 1.3e-11
Identity = 41/65 (63.08%), Postives = 47/65 (72.31%), Query Frame = 1


Query: 51  SPKPTESPPSTSLRSSTHLSGDFSDDVVRQIASASKEWGFFLVVNHGVPLEKRRRIEAAA 110
           SP   +SPP+ S         +F  D+V+QIASA  EWGFFLVVNHGVP EKRRR+EAAA
Sbjct: 35  SPIFNDSPPAGS---------EFPHDLVQQIASACTEWGFFLVVNHGVPPEKRRRMEAAA 90

Query: 111 REFFG 116
           REFFG
Sbjct: 95  REFFG 90

[Close]

BLAST of ClCG02G005620.1 vs. TrEMBL
Match: A0A0A0K3A9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G074960 PE=3 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 6.2e-11
Identity = 36/44 (81.82%), Postives = 37/44 (84.09%), Query Frame = 1


Query: 72  DFSDDVVRQIASASKEWGFFLVVNHGVPLEKRRRIEAAAREFFG 116
           DF  D+V QIASA  EWGFFLVVNHGVP EKR RIEAAAREFFG
Sbjct: 41  DFPHDLVHQIASACTEWGFFLVVNHGVPPEKRHRIEAAAREFFG 84

[Close]

BLAST of ClCG02G005620.1 vs. TrEMBL
Match: A0A0A0K834_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G074940 PE=4 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 4.0e-10
Identity = 35/44 (79.55%), Postives = 36/44 (81.82%), Query Frame = 1


Query: 72  DFSDDVVRQIASASKEWGFFLVVNHGVPLEKRRRIEAAAREFFG 116
           D   D+V QIASA  EWGFFLVVNHGVP EKR RIEAAAREFFG
Sbjct: 46  DVPHDLVHQIASACTEWGFFLVVNHGVPPEKRHRIEAAAREFFG 89

[Close]

BLAST of ClCG02G005620.1 vs. TrEMBL
Match: W9R171_9ROSA (Naringenin,2-oxoglutarate 3-dioxygenase OS=Morus notabilis GN=L484_027018 PE=3 SV=1)

HSP 1 Score: 71.2 bits (173), Expect = 9.0e-10
Identity = 35/47 (74.47%), Postives = 39/47 (82.98%), Query Frame = 1


Query: 69  LSGDFSDD-VVRQIASASKEWGFFLVVNHGVPLEKRRRIEAAAREFF 115
           LSG  +DD +VR+I  A K+WGFF VVNHGVP EKRRRIEAAAREFF
Sbjct: 33  LSGGLTDDELVREIGKACKQWGFFQVVNHGVPAEKRRRIEAAAREFF 79

[Close]

BLAST of ClCG02G005620.1 vs. TAIR10
Match: AT5G08640.1 (AT5G08640.1 flavonol synthase 1)

HSP 1 Score: 52.8 bits (125), Expect = 1.7e-07
Identity = 31/87 (35.63%), Postives = 40/87 (45.98%), Query Frame = 1


Query: 34  ETLRSSIRLSSKTPSSPSPKPTESPPSTSLRSST------HLSGDFSDDVVRQIASASKE 93
           + + SS  L+   P        E P  T+ R  T       LS    + V R +  AS+E
Sbjct: 7   QDISSSSLLTEAIPLEFIRSEKEQPAITTFRGPTPAIPVVDLSDPDEESVRRAVVKASEE 66

Query: 94  WGFFLVVNHGVPLEKRRRIEAAAREFF 115
           WG F VVNHG+P E  RR++   R+FF
Sbjct: 67  WGLFQVVNHGIPTELIRRLQDVGRKFF 93

[Close]

BLAST of ClCG02G005620.1 vs. TAIR10
Match: AT3G19000.1 (AT3G19000.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 50.1 bits (118), Expect = 1.1e-06
Identity = 30/73 (41.10%), Postives = 36/73 (49.32%), Query Frame = 1


Query: 64  RSSTHL--SGDF--SDDV------------------VRQIASASKEWGFFLVVNHGVPLE 115
           R +THL  SGDF  SD++                   ++IA A K WGFF V+NHG+P  
Sbjct: 15  RPNTHLTNSGDFIFSDEIPTIDLSSLEDTHHDKTAIAKEIAEACKRWGFFQVINHGLPSA 74

[Close]

BLAST of ClCG02G005620.1 vs. TAIR10
Match: AT5G63600.2 (AT5G63600.2 flavonol synthase 5)

HSP 1 Score: 48.9 bits (115), Expect = 2.4e-06
Identity = 21/46 (45.65%), Postives = 28/46 (60.87%), Query Frame = 1


Query: 69  LSGDFSDDVVRQIASASKEWGFFLVVNHGVPLEKRRRIEAAAREFF 115
           LS    D +VR++  AS+EWG F VVNHG+P E  R+++    +FF
Sbjct: 38  LSVSDEDFLVREVVKASEEWGVFQVVNHGIPTELMRQLQMVGTQFF 83

[Close]

BLAST of ClCG02G005620.1 vs. TAIR10
Match: AT5G63595.1 (AT5G63595.1 flavonol synthase 4)

HSP 1 Score: 48.9 bits (115), Expect = 2.4e-06
Identity = 24/56 (42.86%), Postives = 30/56 (53.57%), Query Frame = 1


Query: 59  PSTSLRSSTHLSGDFSDDVVRQIASASKEWGFFLVVNHGVPLEKRRRIEAAAREFF 115
           PS+       LS    + V R++A AS+EWG F VVNHG+P E  RR+     +FF
Sbjct: 20  PSSQNFPVVDLSNTNGELVARKVAKASEEWGIFQVVNHGIPTELIRRLHKVDTQFF 75

[Close]

BLAST of ClCG02G005620.1 vs. TAIR10
Match: AT5G43935.1 (AT5G43935.1 flavonol synthase 6)

HSP 1 Score: 47.8 bits (112), Expect = 5.4e-06
Identity = 21/44 (47.73%), Postives = 28/44 (63.64%), Query Frame = 1


Query: 72  DFSDDVVRQ-IASASKEWGFFLVVNHGVPLEKRRRIEAAAREFF 115
           D SD++V   +  AS+EWG F +VNHG+P E  RR++   R+FF
Sbjct: 26  DPSDELVAHAVVKASEEWGIFQLVNHGIPAELMRRLQEVGRQFF 69

[Close]

BLAST of ClCG02G005620.1 vs. NCBI nr
Match: gi|413915332|emb|CBX88044.1| (gibberellin 20-oxidase, partial [Cucumis sativus])

HSP 1 Score: 77.4 bits (189), Expect = 1.8e-11
Identity = 41/65 (63.08%), Postives = 47/65 (72.31%), Query Frame = 1


Query: 51  SPKPTESPPSTSLRSSTHLSGDFSDDVVRQIASASKEWGFFLVVNHGVPLEKRRRIEAAA 110
           SP   +SPP+ S         +F  D+V+QIASA  EWGFFLVVNHGVP EKRRR+EAAA
Sbjct: 35  SPIFNDSPPAGS---------EFPHDLVQQIASACTEWGFFLVVNHGVPPEKRRRMEAAA 90

Query: 111 REFFG 116
           REFFG
Sbjct: 95  REFFG 90

[Close]

BLAST of ClCG02G005620.1 vs. NCBI nr
Match: gi|449438440|ref|XP_004136996.1| (PREDICTED: hyoscyamine 6-dioxygenase-like isoform X2 [Cucumis sativus])

HSP 1 Score: 77.4 bits (189), Expect = 1.8e-11
Identity = 41/65 (63.08%), Postives = 47/65 (72.31%), Query Frame = 1


Query: 51  SPKPTESPPSTSLRSSTHLSGDFSDDVVRQIASASKEWGFFLVVNHGVPLEKRRRIEAAA 110
           SP   +SPP+ S         +F  D+V+QIASA  EWGFFLVVNHGVP EKRRR+EAAA
Sbjct: 35  SPIFNDSPPAGS---------EFPHDLVQQIASACTEWGFFLVVNHGVPPEKRRRMEAAA 90

Query: 111 REFFG 116
           REFFG
Sbjct: 95  REFFG 90

[Close]

BLAST of ClCG02G005620.1 vs. NCBI nr
Match: gi|659109829|ref|XP_008454903.1| (PREDICTED: LOW QUALITY PROTEIN: gibberellin 20 oxidase 1-like [Cucumis melo])

HSP 1 Score: 77.0 bits (188), Expect = 2.4e-11
Identity = 36/44 (81.82%), Postives = 39/44 (88.64%), Query Frame = 1


Query: 72  DFSDDVVRQIASASKEWGFFLVVNHGVPLEKRRRIEAAAREFFG 116
           DF DD+V+QIA+A  EWGFFLVVNHGVP EKR RIEAAAREFFG
Sbjct: 45  DFPDDLVQQIAAACTEWGFFLVVNHGVPPEKRHRIEAAAREFFG 88

[Close]

BLAST of ClCG02G005620.1 vs. NCBI nr
Match: gi|778725230|ref|XP_011658920.1| (PREDICTED: gibberellin 20 oxidase 2-like isoform X3 [Cucumis sativus])

HSP 1 Score: 75.1 bits (183), Expect = 8.9e-11
Identity = 36/44 (81.82%), Postives = 37/44 (84.09%), Query Frame = 1


Query: 72  DFSDDVVRQIASASKEWGFFLVVNHGVPLEKRRRIEAAAREFFG 116
           DF  D+V QIASA  EWGFFLVVNHGVP EKR RIEAAAREFFG
Sbjct: 41  DFPHDLVHQIASACTEWGFFLVVNHGVPPEKRHRIEAAAREFFG 84

[Close]

BLAST of ClCG02G005620.1 vs. NCBI nr
Match: gi|778725233|ref|XP_004136997.2| (PREDICTED: gibberellin 20 oxidase 1-like isoform X4 [Cucumis sativus])

HSP 1 Score: 75.1 bits (183), Expect = 8.9e-11
Identity = 36/44 (81.82%), Postives = 37/44 (84.09%), Query Frame = 1


Query: 72  DFSDDVVRQIASASKEWGFFLVVNHGVPLEKRRRIEAAAREFFG 116
           DF  D+V QIASA  EWGFFLVVNHGVP EKR RIEAAAREFFG
Sbjct: 41  DFPHDLVHQIASACTEWGFFLVVNHGVPPEKRHRIEAAAREFFG 84

The following BLAST results are available for this feature:

BLAST of ClCG02G005620.1 vs. Swiss-Prot


Match Name	E-value	Identity	Description
FLS1_ARATH	3.0e-06	35.63	Flavonol synthase/flavanone 3-hydroxylase OS=Arabidopsis thaliana GN=FLS1 PE=1 S...	[more]

BLAST of ClCG02G005620.1 vs. TrEMBL


Match Name	E-value	Identity	Description
K7ZTB8_CUCSA	1.3e-11	63.08	Gibberellin 20-oxidase (Fragment) OS=Cucumis sativus GN=ga20ox2 PE=2 SV=1	[more]
A0A0A0K6P5_CUCSA	1.3e-11	63.08	Uncharacterized protein OS=Cucumis sativus GN=Csa_7G074950 PE=3 SV=1	[more]
A0A0A0K3A9_CUCSA	6.2e-11	81.82	Uncharacterized protein OS=Cucumis sativus GN=Csa_7G074960 PE=3 SV=1	[more]
A0A0A0K834_CUCSA	4.0e-10	79.55	Uncharacterized protein OS=Cucumis sativus GN=Csa_7G074940 PE=4 SV=1	[more]
W9R171_9ROSA	9.0e-10	74.47	Naringenin,2-oxoglutarate 3-dioxygenase OS=Morus notabilis GN=L484_027018 PE=3 S...	[more]

BLAST of ClCG02G005620.1 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G08640.1	1.7e-07	35.63	flavonol synthase 1	[more]
AT3G19000.1	1.1e-06	41.10	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot...	[more]
AT5G63600.2	2.4e-06	45.65	flavonol synthase 5	[more]
AT5G63595.1	2.4e-06	42.86	flavonol synthase 4	[more]
AT5G43935.1	5.4e-06	47.73	flavonol synthase 6	[more]

BLAST of ClCG02G005620.1 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|413915332\|emb\|CBX88044.1\|	1.8e-11	63.08	gibberellin 20-oxidase, partial [Cucumis sativus]	[more]
gi\|449438440\|ref\|XP_004136996.1\|	1.8e-11	63.08	PREDICTED: hyoscyamine 6-dioxygenase-like isoform X2 [Cucumis sativus]	[more]
gi\|659109829\|ref\|XP_008454903.1\|	2.4e-11	81.82	PREDICTED: LOW QUALITY PROTEIN: gibberellin 20 oxidase 1-like [Cucumis melo]	[more]
gi\|778725230\|ref\|XP_011658920.1\|	8.9e-11	81.82	PREDICTED: gibberellin 20 oxidase 2-like isoform X3 [Cucumis sativus]	[more]
gi\|778725233\|ref\|XP_004136997.2\|	8.9e-11	81.82	PREDICTED: gibberellin 20 oxidase 1-like isoform X4 [Cucumis sativus]	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **INTERPRO**
Term	Definition
IPR026992	DIOX_N
IPR027443	IPNS-like

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003674	molecular_function

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
ClCG02G005620	ClCG02G005620	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
ClCG02G005620.1	ClCG02G005620.1-protein	polypeptide

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
ClCG02G005620.1.cds1	ClCG02G005620.1.cds1	CDS
ClCG02G005620.1.cds2	ClCG02G005620.1.cds2	CDS

InterPro

Analysis Name: InterPro Annotations of watermelon (Charleston Gray)
Date Performed: 2016-09-28


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 72..114 score: 3.6
IPR027443	Isopenicillin N synthase-like	GENE3D	G3DSA:2.60.120.330		coord: 46..114 score: 3.2
None	No IPR available	PANTHER	PTHR10209	OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEIN	coord: 75..114 score: 5.1
None	No IPR available	PANTHER	PTHR10209:SF98	SUBFAMILY NOT NAMED	coord: 75..114 score: 5.1
None	No IPR available	unknown	SSF51197	Clavaminate synthase-like	coord: 45..114 score: 6.87