Csa6G486950.1 (mRNA) Cucumber (Chinese Long) v2 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Csa6G486950.1
Type	mRNA
Organism	Cucumis. sativus (Cucumber (Chinese Long) v2)
Description	Iron--sulfur cluster insertion protein ErpA; contains IPR000361 (FeS cluster biogenesis), IPR016092 (FeS cluster insertion protein)
Location	Chr6 : 22771225 .. 22774373 (-)
Sequence length	525

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAGAAGAGAAGATGAAGAAAAATATCTGAAACGCTCGATCTTAACGAAAACAATATATCTTGACCGCACGATCCCAATACCCAAACATTTTCGACTAGTCAAACACGGCGGTACGCATATTCACTGGTCGTCGTTCACCCTTCGCCGGAAATATCGCGGCAGCACAACCGGGTTTCTCCGATGGCTTTCTCTTCGATTTCCATGAAATGCCCTTGCCCTCCCGTCCTTCACTTGCCAGCTCTTCCTAAAAGTTATGCACCTCAAACTTCTATTTCCTTTCGTTTTCCTTCTACGAAATTCAGCCGGAGAAAATCCTTATCGATTCGATCGGTTTCAGTCCCAGGTACGCTCTAATTTTCAATTTTCCCATCTGTTTTCCGTTATTATACTTGGCTATATCACCTTAGTTCATCGTTGTACTACTTGGGACGAAATTAATCAGGGTTTTGTTTTTCCCTTTTGGATTGGATTGAAATTTGAGGTTGTGGAGAACGTCTGGATTAGAGTTATTTTTTAAAAAAATCAATCAAACGTAGGATTCTTTTGTTGGTTGATTGTAATCGAAATTGATAGCTGGTGGATGTCTTGTGTTGAAATTAAGTAACAGAATTTTGGGACTTTATTGTTGATTTTCATGGAAAATAAATTTCCCCTGTACTTGTACTGACGACTTGTTTATGAGTTATAGTTCAAGATCCAGTTGGTGATATACAAACCGTGAATTTTAGGTCTTTGTTTTGTTCTAATGCCTAAATGATTAGTAGGATGGATTGGCCTTCATGTGGAGGCAAAGGTGTTTACTTTTTCTTGTGTTTGATACCATTGATTGGTTGTTTGTTTTAGAGCCTGTTTGGGAGTGGTTTTGAAATGGTTAATGGCTTTCTTTCATGTTCAAAACCACTCCGGAATTTGTCTTTAATCATTAAAAAAAGATTTAATGTTCGATAATTTCACTTTTAAATGCAATTTCCATATCTCTAAATTTAATTTTGAAGGATTAAAAGCATATTTCAAGTCATTTTGGATATGAAAAGTGATTTTAACCCTTTCAAAATAAGTCCAAACATGCCCTTTTATATCTTCTGGTGCAAATTATTTGTTGTTTTCTCTATGTCACTTTCCCTTTGCCTGTTACTGCAGATCAAAAGATAAAACCTAATGAATCTTGTCCTATAGTTTTGGCATATGAAATACGATGAGTATATCATGCAGTTATGATCAAAGTTTGCCTCGTCATCTTATGTTTTTTGTAATTAAACAGCTGCGCCAGCATCTGAGGGTATAGCACCTGCTATCTCATTAACCGATACTGCATTGAAACACTTGAATAAAATGAGATCTGAACGTAATGAAGATTTGTGCTTAAGAATTGGTGTCAGACAGGGTGGATGCTCTGGTATGTCTTACACCATGGAGTTTGAGAGCAGGGCAAATGCTAGACCTGATGACTCGATTATAGAATATAATGGCTTTGTGATAGGTAAGAGTTATGGCTTTGCTATTTTTCACCTTGAATTTCTAGTGAGATTTTTACTAGTGATAGTGCAACTGTTTGAAGCAATCCTTTTTTTGAACCCATTGCTTTATATACATGTGTATGTGTGTGGGTGGGTGGGGGAGGGGGGTGTGAGGATAGTGGAAAGTCCAAGTCTTGTTTTGAACATGTGTTGAATTTTGATACTGTGGAATGGTAAGGGCAAGTTTTCAAATTTCCTTAGGATGAAAGAGGCTTACTCTTTTATAGTTCTATACATTCAGATAGTGATGGATATTTCATGAACCATATGCCATAAATTTGAAGGACTCTGCAAGAATAATTTTTCTCTATAGTTCATAATCTACCTCTTAGCTCTAAAAGAAAACGGTGGTATACATAAGTCTAGGTTTGCATTTTTTTTATGAATCAAACTGCAAAAGGAAAGAATTCCAACACAAAGAATACAAGGGCGTGAAAAAAATCCAGCCCACCAAAACACCCCAACCATCGTAAGGAGGACCAACTAAGTAAAATGTTACCAATAGAGTAATTACAAAAATGCTTTGATATTGAAGCCGACTATGCTCCTTCTCCCTACCTCTAAAGACTCGATCGTTCCTCTCACCCCAAATGTCTCAAACAATAGTACACACCCCAACCAACAATAAAAATCCCCCTTTATCATTGAAAGGCAGATGGAGGAGGAACTCCTTGATCGTTGCTCTGTCACTCCTAGAGCCAACAAAACAAACACCCAACTCCTGCAGGAAAGAACTCCAAACTGCCCTTGCATAATGAGAGTCCCAGAAAAGAAGTCTAGGTTTGCATATTGTTAGATTGATGCTTTCTTTCCCGTGGATCGGTAGAGGACAAGTTTTCAATTTTTATTAGGATGAAAGAAGCTTACATATCATGAGCTGCGTGCCATAAATTTGCTGGACTCTATAAGAAGTTTTACTCTATAGCTCATAATCTATCACTTCTCTTCGACACTCAATCACTTGAATTATTTGATATGAGCAATTTTTTATGCTAACTGATAAGTAGGTTGCTCGACAACTTTTGTCAAATATATTTTTCTTTAATGCATGCTATTCAACATAAAGTGGTATATTGTTTCAATTCCTTGATAATTGCCAAAGTGATAAAAAAGTTTGTAATTCAAAACTTCGAGTCCTTCCACCCTCTTACCAATGAAAAGAGTGTTCTTGATAATTGCTCTCTACTCTATTGATAAAGGAAAGTTAGCATCTTTTAGTGAACTATGAAAGGATTTTTTTATCTACTTCATTTTCCTCTATGCCCTTTTAAGTTGTACCCTCGAACACGAATTTAAGTTCACTTTTAGTTTGCACTTTTGATACAAATTTCCCTCTCTTCTTGTTTTGTAGTATGTGATCCCAAGAGCCTACTTTTCTTGTTTGGAATGCAATTGGACTACAGCGACGCACTTATTGGTGGAGGATTTTCTTTCAAGAACCCAAATGCTACACAAACTTGTGGTTGTGGAAAATCATTTAATGCAGAAATGTAGGACCATATTTTGTTTATATGAATGATTGAATAGAAACTTGATAGTTTCAGGTTAAAGAAAACTTAGAATGTATAAAAACTCTTACATTGCTTGACAATTGATCCTAATAGTATACCTTCTATTACTAGTCGTTGGTGA

mRNA sequence

ATGGCTTTCTCTTCGATTTCCATGAAATGCCCTTGCCCTCCCGTCCTTCACTTGCCAGCTCTTCCTAAAAGTTATGCACCTCAAACTTCTATTTCCTTTCGTTTTCCTTCTACGAAATTCAGCCGGAGAAAATCCTTATCGATTCGATCGGTTTCAGTCCCAGCTGCGCCAGCATCTGAGGGTATAGCACCTGCTATCTCATTAACCGATACTGCATTGAAACACTTGAATAAAATGAGATCTGAACGTAATGAAGATTTGTGCTTAAGAATTGGTGTCAGACAGGGTGGATGCTCTGGTATGTCTTACACCATGGAGTTTGAGAGCAGGGCAAATGCTAGACCTGATGACTCGATTATAGAATATAATGGCTTTGTGATAGTATGTGATCCCAAGAGCCTACTTTTCTTGTTTGGAATGCAATTGGACTACAGCGACGCACTTATTGGTGGAGGATTTTCTTTCAAGAACCCAAATGCTACACAAACTTGTGGTTGTGGAAAATCATTTAATGCAGAAATGTAG

Coding sequence (CDS)

ATGGCTTTCTCTTCGATTTCCATGAAATGCCCTTGCCCTCCCGTCCTTCACTTGCCAGCTCTTCCTAAAAGTTATGCACCTCAAACTTCTATTTCCTTTCGTTTTCCTTCTACGAAATTCAGCCGGAGAAAATCCTTATCGATTCGATCGGTTTCAGTCCCAGCTGCGCCAGCATCTGAGGGTATAGCACCTGCTATCTCATTAACCGATACTGCATTGAAACACTTGAATAAAATGAGATCTGAACGTAATGAAGATTTGTGCTTAAGAATTGGTGTCAGACAGGGTGGATGCTCTGGTATGTCTTACACCATGGAGTTTGAGAGCAGGGCAAATGCTAGACCTGATGACTCGATTATAGAATATAATGGCTTTGTGATAGTATGTGATCCCAAGAGCCTACTTTTCTTGTTTGGAATGCAATTGGACTACAGCGACGCACTTATTGGTGGAGGATTTTCTTTCAAGAACCCAAATGCTACACAAACTTGTGGTTGTGGAAAATCATTTAATGCAGAAATGTAG

Protein sequence

MAFSSISMKCPCPPVLHLPALPKSYAPQTSISFRFPSTKFSRRKSLSIRSVSVPAAPASEGIAPAISLTDTALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFESRANARPDDSIIEYNGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFNAEM*

Homology

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BLAST of Csa6G486950.1 vs. Swiss-Prot
Match: ISCAP_ARATH (Iron-sulfur assembly protein IscA, chloroplastic OS=Arabidopsis thaliana GN=ISCA PE=2 SV=2)

HSP 1 Score: 238.0 bits (606), Expect = 7.6e-62
Identity = 116/148 (78.38%), Postives = 125/148 (84.46%), Query Frame = 1


Query: 32  SFRFPSTKF-----SRRKSLSIRSVSVPAAPASEGIAPAISLTDTALKHLNKMRSERNED 91
           S  FPS  +     +RR  LS+RS SVPAAPA EG+ PAISL++ ALKHL+KMRSER ED
Sbjct: 33  SISFPSLSYVNLNLNRRNRLSVRSASVPAAPAMEGLKPAISLSENALKHLSKMRSERGED 92

Query: 92  LCLRIGVRQGGCSGMSYTMEFESRANARPDDSIIEYNGFVIVCDPKSLLFLFGMQLDYSD 151
           LCLRIGV+QGGCSGMSYTM+FE+RANARPDDS IEY GF IVCDPKS+LFLFGMQLDYSD
Sbjct: 93  LCLRIGVKQGGCSGMSYTMDFENRANARPDDSTIEYQGFTIVCDPKSMLFLFGMQLDYSD 152

Query: 152 ALIGGGFSFKNPNATQTCGCGKSFNAEM 175
           ALIGGGFSF NPNATQTCGCGKSF AEM
Sbjct: 153 ALIGGGFSFSNPNATQTCGCGKSFAAEM 180

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BLAST of Csa6G486950.1 vs. Swiss-Prot
Match: Y1417_SYNY3 (Uncharacterized protein slr1417 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) GN=slr1417 PE=3 SV=1)

HSP 1 Score: 152.1 bits (383), Expect = 5.5e-36
Identity = 68/108 (62.96%), Postives = 81/108 (75.00%), Query Frame = 1


Query: 63  APAISLTDTALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFESRANARPDDSIIEY 122
           A  I L+D ALKHL  ++ ++ +DLCLR+GVRQGGCSGMSY M+FE    A   D + +Y
Sbjct: 9   AKGIQLSDAALKHLLALKEQQGKDLCLRVGVRQGGCSGMSYMMDFEEPNRATEHDEVFDY 68

Query: 123 NGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSF 171
            GF I+CD KSLL+L+G+ LDYS+ALIGGGF F NPNA QTCGCGKSF
Sbjct: 69  EGFQIICDRKSLLYLYGLMLDYSNALIGGGFQFTNPNANQTCGCGKSF 116

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BLAST of Csa6G486950.1 vs. Swiss-Prot
Match: YCF83_PORPU (Uncharacterized protein ycf83 OS=Porphyra purpurea GN=ycf83 PE=3 SV=1)

HSP 1 Score: 139.4 bits (350), Expect = 3.7e-32
Identity = 60/106 (56.60%), Postives = 81/106 (76.42%), Query Frame = 1


Query: 66  ISLTDTALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFESRANARPDDSIIEYNGF 125
           I+LT+ A + +  ++S  + ++CLRIGV+QGGCSGMSY+M FES ++ R  D ++  + F
Sbjct: 8   ITLTEPAARQIKILKSNFDNEVCLRIGVKQGGCSGMSYSMNFESVSSLRETDEVLMLDSF 67

Query: 126 VIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFN 172
           ++ CDPKSLL+L+G+ LDYS  LIGGGF F NPNA+QTCGCGKSF+
Sbjct: 68  LVACDPKSLLYLYGLSLDYSSELIGGGFQFSNPNASQTCGCGKSFS 113

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BLAST of Csa6G486950.1 vs. Swiss-Prot
Match: YCF83_PYRYE (Uncharacterized protein ycf83 OS=Pyropia yezoensis GN=ycf83 PE=3 SV=1)

HSP 1 Score: 132.1 bits (331), Expect = 5.9e-30
Identity = 61/112 (54.46%), Postives = 78/112 (69.64%), Query Frame = 1


Query: 60  EGIAPAISLTDTALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFESRANARPDDSI 119
           EG    I++T  ALK +  ++++   D+ LRIGVRQGGCSGMSY+M FE     +  D  
Sbjct: 2   EGNMGFITITKPALKQIAILKNDHENDVHLRIGVRQGGCSGMSYSMNFEHVDKLKDTDER 61

Query: 120 IEYNGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFN 172
           +  + F +VCDPKSLL+L+G+ LD+S  LIGGGF F NPNA+QTCGCGKSF+
Sbjct: 62  LRLDNFSVVCDPKSLLYLYGLSLDFSSELIGGGFQFSNPNASQTCGCGKSFS 113

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BLAST of Csa6G486950.1 vs. Swiss-Prot
Match: YCF83_GALSU (Uncharacterized protein ycf83 OS=Galdieria sulphuraria GN=ycf83 PE=3 SV=1)

HSP 1 Score: 130.2 bits (326), Expect = 2.2e-29
Identity = 58/107 (54.21%), Postives = 79/107 (73.83%), Query Frame = 1


Query: 66  ISLTDTALKHLNKMRSERNED-LCLRIGVRQGGCSGMSYTMEFESRANARPDDSIIEYNG 125
           I++T +AL HLN +    +ED L +RIG++QGGCSG+SY M +E ++N   +D + +Y+ 
Sbjct: 6   INITKSALDHLNSISKLNSEDKLYIRIGIKQGGCSGLSYFMTYEKKSNISDNDLVYDYDN 65

Query: 126 FVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFN 172
           F +VCD KS+L+L+G+ LDYS +LI GGF F NPNA QTCGCGKSF+
Sbjct: 66  FTLVCDNKSILYLYGISLDYSSSLIDGGFKFLNPNAKQTCGCGKSFS 112

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BLAST of Csa6G486950.1 vs. TrEMBL
Match: A0A0A0KJ66_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G486950 PE=4 SV=1)

HSP 1 Score: 354.4 bits (908), Expect = 8.1e-95
Identity = 174/174 (100.00%), Postives = 174/174 (100.00%), Query Frame = 1


Query: 1   MAFSSISMKCPCPPVLHLPALPKSYAPQTSISFRFPSTKFSRRKSLSIRSVSVPAAPASE 60
           MAFSSISMKCPCPPVLHLPALPKSYAPQTSISFRFPSTKFSRRKSLSIRSVSVPAAPASE
Sbjct: 1   MAFSSISMKCPCPPVLHLPALPKSYAPQTSISFRFPSTKFSRRKSLSIRSVSVPAAPASE 60

Query: 61  GIAPAISLTDTALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFESRANARPDDSII 120
           GIAPAISLTDTALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFESRANARPDDSII
Sbjct: 61  GIAPAISLTDTALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFESRANARPDDSII 120

Query: 121 EYNGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFNAEM 175
           EYNGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFNAEM
Sbjct: 121 EYNGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFNAEM 174

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BLAST of Csa6G486950.1 vs. TrEMBL
Match: M5WD48_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa012351mg PE=4 SV=1)

HSP 1 Score: 272.7 bits (696), Expect = 3.1e-70
Identity = 136/175 (77.71%), Postives = 149/175 (85.14%), Query Frame = 1


Query: 1   MAFSSISMKCPCPPVLHLPA-LPKSYAPQTSISFRFPSTKFSRRKSLSIRSVSVPAAPAS 60
           MAFS+I+  CP   +L  P  LP +++P  SISFRF     SRRK LSIRS S  AAP +
Sbjct: 1   MAFSAITTHCPS--LLRPPTNLPVTFSPPNSISFRFSKNNLSRRKPLSIRSTSTSAAPKT 60

Query: 61  EGIAPAISLTDTALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFESRANARPDDSI 120
           EG+APAISLTD ALKH NKMRSER EDLCLRIGV+QGGCSGMSYTM+FESRANARPDDS+
Sbjct: 61  EGLAPAISLTDNALKHFNKMRSERGEDLCLRIGVKQGGCSGMSYTMDFESRANARPDDSV 120

Query: 121 IEYNGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFNAEM 175
           IEYNGFV+VCDPKSLLFL+GMQLDYSDALIGGGFSFKNPNATQTCGCGKSF AEM
Sbjct: 121 IEYNGFVMVCDPKSLLFLYGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEM 173

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BLAST of Csa6G486950.1 vs. TrEMBL
Match: A0A140B490_PRUPE (Fe-S cluster assembly protein OS=Prunus persica var. persica GN=ISA2 PE=2 SV=1)

HSP 1 Score: 272.7 bits (696), Expect = 3.1e-70
Identity = 136/175 (77.71%), Postives = 149/175 (85.14%), Query Frame = 1


Query: 1   MAFSSISMKCPCPPVLHLPA-LPKSYAPQTSISFRFPSTKFSRRKSLSIRSVSVPAAPAS 60
           MAFS+I+  CP   +L  P  LP +++P  SISFRF     SRRK LSIRS S  AAP +
Sbjct: 1   MAFSAITTHCPS--LLRPPTNLPVTFSPPNSISFRFSKNNLSRRKPLSIRSTSTSAAPKT 60

Query: 61  EGIAPAISLTDTALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFESRANARPDDSI 120
           EG+APAISLTD ALKH NKMRSER EDLCLRIGV+QGGCSGMSYTM+FESRANARPDDS+
Sbjct: 61  EGLAPAISLTDNALKHFNKMRSERGEDLCLRIGVKQGGCSGMSYTMDFESRANARPDDSV 120

Query: 121 IEYNGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFNAEM 175
           IEYNGFV+VCDPKSLLFL+GMQLDYSDALIGGGFSFKNPNATQTCGCGKSF AEM
Sbjct: 121 IEYNGFVMVCDPKSLLFLYGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEM 173

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BLAST of Csa6G486950.1 vs. TrEMBL
Match: A0A067KGP6_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_09426 PE=4 SV=1)

HSP 1 Score: 267.3 bits (682), Expect = 1.3e-68
Identity = 135/176 (76.70%), Postives = 147/176 (83.52%), Query Frame = 1


Query: 1   MAFSSISMKCPCPPVLHLPALPK--SYAPQTSISFRFPSTKFSRRKSLSIRSVSVPAAPA 60
           MAFS        P +L LP   K  S +   S+SFRF  +  SR+ SLS+RS S P APA
Sbjct: 1   MAFSGTVTTPHRPSILRLPCHSKTTSVSSHDSVSFRFSRSNLSRKISLSVRSTSSPVAPA 60

Query: 61  SEGIAPAISLTDTALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFESRANARPDDS 120
           +EGIAPAISLT+ ALKHLNKMR++RNEDLCLRIGV+QGGCSGMSYTMEFE+RANARPDDS
Sbjct: 61  TEGIAPAISLTENALKHLNKMRNDRNEDLCLRIGVKQGGCSGMSYTMEFENRANARPDDS 120

Query: 121 IIEYNGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFNAEM 175
           IIEYNGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSF AEM
Sbjct: 121 IIEYNGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEM 176

[Close]

BLAST of Csa6G486950.1 vs. TrEMBL
Match: A0A061DJ29_THECC (Chloroplast-localized ISCA-like protein OS=Theobroma cacao GN=TCM_001267 PE=4 SV=1)

HSP 1 Score: 260.8 bits (665), Expect = 1.2e-66
Identity = 128/174 (73.56%), Postives = 144/174 (82.76%), Query Frame = 1


Query: 1   MAFSSISMKCPCPPVLHLPALPKSYAPQTSISFRFPSTKFSRRKSLSIRSVSVPAAPASE 60
           MA SSI+  CP        +     +PQ S+SFRF  +  S  K LS+R+ +VPAAP SE
Sbjct: 1   MAISSITTNCPSLLRFSNNSKQSFSSPQISVSFRFSRSSLSCGKPLSVRATTVPAAPTSE 60

Query: 61  GIAPAISLTDTALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFESRANARPDDSII 120
           G+APAI+LTD ALKHLNKMR+ER+EDLCLRIGV+QGGCSGMSYTM+FE RA+ARPDDSII
Sbjct: 61  GLAPAITLTDDALKHLNKMRAERDEDLCLRIGVKQGGCSGMSYTMDFEKRASARPDDSII 120

Query: 121 EYNGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFNAEM 175
           EYNGF I+CDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSF AEM
Sbjct: 121 EYNGFTIICDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEM 174

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BLAST of Csa6G486950.1 vs. TAIR10
Match: AT1G10500.1 (AT1G10500.1 chloroplast-localized ISCA-like protein)

HSP 1 Score: 238.0 bits (606), Expect = 4.3e-63
Identity = 116/148 (78.38%), Postives = 125/148 (84.46%), Query Frame = 1


Query: 32  SFRFPSTKF-----SRRKSLSIRSVSVPAAPASEGIAPAISLTDTALKHLNKMRSERNED 91
           S  FPS  +     +RR  LS+RS SVPAAPA EG+ PAISL++ ALKHL+KMRSER ED
Sbjct: 33  SISFPSLSYVNLNLNRRNRLSVRSASVPAAPAMEGLKPAISLSENALKHLSKMRSERGED 92

Query: 92  LCLRIGVRQGGCSGMSYTMEFESRANARPDDSIIEYNGFVIVCDPKSLLFLFGMQLDYSD 151
           LCLRIGV+QGGCSGMSYTM+FE+RANARPDDS IEY GF IVCDPKS+LFLFGMQLDYSD
Sbjct: 93  LCLRIGVKQGGCSGMSYTMDFENRANARPDDSTIEYQGFTIVCDPKSMLFLFGMQLDYSD 152

Query: 152 ALIGGGFSFKNPNATQTCGCGKSFNAEM 175
           ALIGGGFSF NPNATQTCGCGKSF AEM
Sbjct: 153 ALIGGGFSFSNPNATQTCGCGKSFAAEM 180

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BLAST of Csa6G486950.1 vs. TAIR10
Match: AT5G03905.1 (AT5G03905.1 Iron-sulphur cluster biosynthesis family protein)

HSP 1 Score: 77.0 bits (188), Expect = 1.3e-14
Identity = 45/136 (33.09%), Postives = 65/136 (47.79%), Query Frame = 1


Query: 40  FSRRKSLSIRSVSVPAAPASEGIAPAISLTDTALKHLNKMRSERNEDLCLRIGVRQGGCS 99
           FS   ++   S S  + P S      + L+D  ++ + +++S   E   LR+GV  GGCS
Sbjct: 26  FSSASAIKEASSSSSSQPESSS-NDVVHLSDNCIRRMKELQSSEPEKKMLRLGVETGGCS 85

Query: 100 GMSYTMEFESRANARPDDSIIEYNGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFK-NP 159
           G  Y  E ++R N  PDD + E NG  +V D  S   + G  +DY + LI   F    NP
Sbjct: 86  GFQYKFELDNRTN--PDDRVFEKNGVKLVVDNVSYDLVKGATIDYEEELIRAAFVVAVNP 145

Query: 160 NATQTCGCGKSFNAEM 175
           +A   C C  SF  ++
Sbjct: 146 SAVGGCSCKSSFMVKL 158

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BLAST of Csa6G486950.1 vs. NCBI nr
Match: gi|449448422|ref|XP_004141965.1| (PREDICTED: iron-sulfur assembly protein IscA, chloroplastic [Cucumis sativus])

HSP 1 Score: 354.4 bits (908), Expect = 1.2e-94
Identity = 174/174 (100.00%), Postives = 174/174 (100.00%), Query Frame = 1


Query: 1   MAFSSISMKCPCPPVLHLPALPKSYAPQTSISFRFPSTKFSRRKSLSIRSVSVPAAPASE 60
           MAFSSISMKCPCPPVLHLPALPKSYAPQTSISFRFPSTKFSRRKSLSIRSVSVPAAPASE
Sbjct: 1   MAFSSISMKCPCPPVLHLPALPKSYAPQTSISFRFPSTKFSRRKSLSIRSVSVPAAPASE 60

Query: 61  GIAPAISLTDTALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFESRANARPDDSII 120
           GIAPAISLTDTALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFESRANARPDDSII
Sbjct: 61  GIAPAISLTDTALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFESRANARPDDSII 120

Query: 121 EYNGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFNAEM 175
           EYNGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFNAEM
Sbjct: 121 EYNGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFNAEM 174

[Close]

BLAST of Csa6G486950.1 vs. NCBI nr
Match: gi|659079202|ref|XP_008440129.1| (PREDICTED: iron-sulfur assembly protein IscA, chloroplastic [Cucumis melo])

HSP 1 Score: 314.7 bits (805), Expect = 1.0e-82
Identity = 160/174 (91.95%), Postives = 164/174 (94.25%), Query Frame = 1


Query: 1   MAFSSISMKCPCPPVLHLPALPKSYAPQTSISFRFPSTKFSRRKSLSIRSVSVPAAPASE 60
           MAFSSI+MK P  PVLHL ALPKS APQ+SISFRF  TKFSR KSLSIRSVSVPAAPASE
Sbjct: 1   MAFSSITMKFP--PVLHLRALPKSSAPQSSISFRFSPTKFSRSKSLSIRSVSVPAAPASE 60

Query: 61  GIAPAISLTDTALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFESRANARPDDSII 120
            IAPAISLTD ALKHLNKMRSER+EDLCLRIGVRQGGCSGMSYTMEFE+RANARPDDSII
Sbjct: 61  SIAPAISLTDNALKHLNKMRSERDEDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSII 120

Query: 121 EYNGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFNAEM 175
           EYNGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFNAEM
Sbjct: 121 EYNGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFNAEM 172

[Close]

BLAST of Csa6G486950.1 vs. NCBI nr
Match: gi|595830129|ref|XP_007206009.1| (hypothetical protein PRUPE_ppa012351mg [Prunus persica])

HSP 1 Score: 272.7 bits (696), Expect = 4.4e-70
Identity = 136/175 (77.71%), Postives = 149/175 (85.14%), Query Frame = 1


Query: 1   MAFSSISMKCPCPPVLHLPA-LPKSYAPQTSISFRFPSTKFSRRKSLSIRSVSVPAAPAS 60
           MAFS+I+  CP   +L  P  LP +++P  SISFRF     SRRK LSIRS S  AAP +
Sbjct: 1   MAFSAITTHCPS--LLRPPTNLPVTFSPPNSISFRFSKNNLSRRKPLSIRSTSTSAAPKT 60

Query: 61  EGIAPAISLTDTALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFESRANARPDDSI 120
           EG+APAISLTD ALKH NKMRSER EDLCLRIGV+QGGCSGMSYTM+FESRANARPDDS+
Sbjct: 61  EGLAPAISLTDNALKHFNKMRSERGEDLCLRIGVKQGGCSGMSYTMDFESRANARPDDSV 120

Query: 121 IEYNGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFNAEM 175
           IEYNGFV+VCDPKSLLFL+GMQLDYSDALIGGGFSFKNPNATQTCGCGKSF AEM
Sbjct: 121 IEYNGFVMVCDPKSLLFLYGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEM 173

[Close]

BLAST of Csa6G486950.1 vs. NCBI nr
Match: gi|645218786|ref|XP_008232424.1| (PREDICTED: iron-sulfur assembly protein IscA, chloroplastic [Prunus mume])

HSP 1 Score: 268.1 bits (684), Expect = 1.1e-68
Identity = 137/177 (77.40%), Postives = 149/177 (84.18%), Query Frame = 1


Query: 1   MAFSSISMKCPCPPVLHLPA-LPKSYAPQTSISFRFPSTKFSRRKSLSIRSVSVP--AAP 60
           MAFS+I+  CP   +L LP  LP + +P  SISFRF     SRRK LSIRS S    AAP
Sbjct: 1   MAFSAITTHCPS--LLRLPTNLPVTSSPLNSISFRFSKNNLSRRKPLSIRSTSTSTSAAP 60

Query: 61  ASEGIAPAISLTDTALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFESRANARPDD 120
            +EG+APAISLTD ALKH NKMRSER EDLCLRIGV+QGGCSGMSYTM+FESRANARPDD
Sbjct: 61  KTEGLAPAISLTDNALKHFNKMRSERGEDLCLRIGVKQGGCSGMSYTMDFESRANARPDD 120

Query: 121 SIIEYNGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFNAEM 175
           S+IEYNGFV+VCDPKSLLFL+GMQLDYSDALIGGGFSFKNPNATQTCGCGKSF AEM
Sbjct: 121 SVIEYNGFVMVCDPKSLLFLYGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEM 175

[Close]

BLAST of Csa6G486950.1 vs. NCBI nr
Match: gi|1009146759|ref|XP_015891043.1| (PREDICTED: iron-sulfur assembly protein IscA, chloroplastic [Ziziphus jujuba])

HSP 1 Score: 268.1 bits (684), Expect = 1.1e-68
Identity = 136/177 (76.84%), Postives = 150/177 (84.75%), Query Frame = 1


Query: 1   MAFSSISMKCPCPPVLHLPA-LPKSYAPQTSISFRFPSTKFSRRKSLSIRSVSVPAAPA- 60
           MAFS+I+M CP   VL LP   P +  PQ S S RF   K +RRK +SIRS SVPAAPA 
Sbjct: 1   MAFSAITMHCPS--VLRLPNNQPSNSLPQNSFSLRFSPLKLNRRKPISIRSTSVPAAPAP 60

Query: 61  -SEGIAPAISLTDTALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFESRANARPDD 120
            SEG+APAI L+D+ALKHLN MRSERNEDLCLRIGV+QGGCSGMSYTMEFE+RAN RPDD
Sbjct: 61  TSEGLAPAILLSDSALKHLNTMRSERNEDLCLRIGVKQGGCSGMSYTMEFENRANTRPDD 120

Query: 121 SIIEYNGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFNAEM 175
           S+IEY GF+IVCDPKSLLF++GMQLDYSDALIGGGFSFKNPNATQTCGCGKSF AEM
Sbjct: 121 SVIEYKGFIIVCDPKSLLFVYGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEM 175

The following BLAST results are available for this feature:

BLAST of Csa6G486950.1 vs. Swiss-Prot


Match Name	E-value	Identity	Description
ISCAP_ARATH	7.6e-62	78.38	Iron-sulfur assembly protein IscA, chloroplastic OS=Arabidopsis thaliana GN=ISCA...	[more]
Y1417_SYNY3	5.5e-36	62.96	Uncharacterized protein slr1417 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) ...	[more]
YCF83_PORPU	3.7e-32	56.60	Uncharacterized protein ycf83 OS=Porphyra purpurea GN=ycf83 PE=3 SV=1	[more]
YCF83_PYRYE	5.9e-30	54.46	Uncharacterized protein ycf83 OS=Pyropia yezoensis GN=ycf83 PE=3 SV=1	[more]
YCF83_GALSU	2.2e-29	54.21	Uncharacterized protein ycf83 OS=Galdieria sulphuraria GN=ycf83 PE=3 SV=1	[more]

BLAST of Csa6G486950.1 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KJ66_CUCSA	8.1e-95	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_6G486950 PE=4 SV=1	[more]
M5WD48_PRUPE	3.1e-70	77.71	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa012351mg PE=4 SV=1	[more]
A0A140B490_PRUPE	3.1e-70	77.71	Fe-S cluster assembly protein OS=Prunus persica var. persica GN=ISA2 PE=2 SV=1	[more]
A0A067KGP6_JATCU	1.3e-68	76.70	Uncharacterized protein OS=Jatropha curcas GN=JCGZ_09426 PE=4 SV=1	[more]
A0A061DJ29_THECC	1.2e-66	73.56	Chloroplast-localized ISCA-like protein OS=Theobroma cacao GN=TCM_001267 PE=4 SV...	[more]

BLAST of Csa6G486950.1 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G10500.1	4.3e-63	78.38	chloroplast-localized ISCA-like protein	[more]
AT5G03905.1	1.3e-14	33.09	Iron-sulphur cluster biosynthesis family protein	[more]

BLAST of Csa6G486950.1 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449448422\|ref\|XP_004141965.1\|	1.2e-94	100.00	PREDICTED: iron-sulfur assembly protein IscA, chloroplastic [Cucumis sativus]	[more]
gi\|659079202\|ref\|XP_008440129.1\|	1.0e-82	91.95	PREDICTED: iron-sulfur assembly protein IscA, chloroplastic [Cucumis melo]	[more]
gi\|595830129\|ref\|XP_007206009.1\|	4.4e-70	77.71	hypothetical protein PRUPE_ppa012351mg [Prunus persica]	[more]
gi\|645218786\|ref\|XP_008232424.1\|	1.1e-68	77.40	PREDICTED: iron-sulfur assembly protein IscA, chloroplastic [Prunus mume]	[more]
gi\|1009146759\|ref\|XP_015891043.1\|	1.1e-68	76.84	PREDICTED: iron-sulfur assembly protein IscA, chloroplastic [Ziziphus jujuba]	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **INTERPRO**
Term	Definition
IPR000361	FeS_biogenesis
IPR016092	FeS_cluster_insertion
IPR017870	FeS_cluster_insertion_CS

Vocabulary: **Molecular Function**
Term	Definition
GO:0005198	structural molecule activity
GO:0051536	iron-sulfur cluster binding

Vocabulary: **Biological Process**
Term	Definition
GO:0016226	iron-sulfur cluster assembly

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016226	iron-sulfur cluster assembly
cellular_component	GO:0009570	chloroplast stroma
molecular_function	GO:0051536	iron-sulfur cluster binding
molecular_function	GO:0005198	structural molecule activity

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Csa6G486950	Csa6G486950	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Csa6G486950.1	Csa6G486950.1-protein	polypeptide

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Csa6G486950.1.utr3p1	Csa6G486950.1.utr3p1	three_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Csa6G486950.1.cds3	Csa6G486950.1.cds3	CDS
Csa6G486950.1.cds2	Csa6G486950.1.cds2	CDS
Csa6G486950.1.cds1	Csa6G486950.1.cds1	CDS

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Csa6G486950.1.utr5p1	Csa6G486950.1.utr5p1	five_prime_UTR

InterPro

Analysis Name: InterPro Annotations of cucumber (Chinese Long)
Date Performed: 2016-09-28


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000361	FeS cluster biogenesis	GENE3D	G3DSA:2.60.300.12		coord: 66..161 score: 1.1
IPR000361	FeS cluster biogenesis	PFAM	PF01521	Fe-S_biosyn	coord: 66..168 score: 1.1
IPR000361	FeS cluster biogenesis	unknown	SSF89360	HesB-like domain	coord: 65..161 score: 1.96
IPR016092	FeS cluster insertion protein	TIGRFAMs	TIGR00049	TIGR00049	coord: 66..171 score: 4.9
IPR017870	FeS cluster insertion, C-terminal, conserved site	PROSITE	PS01152	HESB	coord: 153..170 scor
None	No IPR available	PANTHER	PTHR10072	IRON-SULFUR CLUSTER ASSEMBLY PROTEIN	coord: 39..173 score: 8.6