WMU64657 (transcribed_cluster) Watermelon (97103) v1

NameWMU64657
Typetranscribed_cluster
OrganismCitrullus. lanatus (Watermelon (97103) v1)
DescriptionUnknown protein
LocationChr4 : 13148190 .. 13159200 (+)
Sequence length428
The following sequences are available for this feature:

transcribed_cluster sequence

TCTTCCAACAGAATATGCCTTATTGTCTTGTTGTCTACTTATTTGTTTGCCAATTGGTTCCACTCGATCACCATTTATCTGAATGTCCAGTTTTATCAACTCACTTTCTCTGTACCCAATAAAAGAATATTCCATGCTCGCATAACCTTTGCTTCTGGATTTCAGTTGGTCAAAGAAATCACCTACCATTTTCGGCTAGCGGCAGAGCGTAGGTGATTGATGCTCTAATTTCTGTAATAAATTTTCATTTTCTTGAAACTCTCCTCTTCTTTCCTGGGCTAATTCCATCAGGGGTCCAATATACTCTTTTGGTGTAAGCATCTCAATCTTGACAAATGGCTCTTCAATTGACTTCCTTTTTCCAGGTTCAGGAAGTATTGATGGGATTTTGAGCCATTCAACAGTATCACCGTTTTACGCCAGTTTAC
BLAST of WMU64657 vs. TAIR10
Match: AT5G08650.1 (AT5G08650.1 Small GTP-binding protein)

HSP 1 Score: 160.6 bits (405), Expect = 7.1e-40
Identity = 89/140 (63.57%), Postives = 106/140 (75.71%), Query Frame = -3

Query: 1   NGDTVEWLKIPSILPEPGKRKSIEEPFVKIEMLTPKEYIGPLMELAQERRGEFQENENLL 60
           NGDT      PS LP+PG+RKS+EEP+VKIE+LTPK+YIG LMELAQERRGEF+E + + 
Sbjct: 459 NGDTT-LCSNPSRLPDPGQRKSVEEPYVKIELLTPKDYIGALMELAQERRGEFKEMKYIA 518

Query: 61  QKLEHQSPTLCR*P--KMVGDFFDQLKSRSKGYASMEYSFIGYRESELIKLDIQINGDRV 120
              E+++  L   P  +MVGDFFDQLKSR+KGYASMEYS IGYRES+LIKLDI IN + V
Sbjct: 519 ---ENRASILYELPLAEMVGDFFDQLKSRTKGYASMEYSVIGYRESDLIKLDILINAEMV 578

Query: 121 EPIGKQISRQQDNKAYSVGR 180
           EP+   + R   +KAYSVGR
Sbjct: 579 EPLSTIVHR---DKAYSVGR 591

Query: 181 240
           
Sbjct: 639 591

Query: 241 300
           
Sbjct: 699 591

Query: 301 360
           
Sbjct: 759 591

Query: 361 415
           
Sbjct: 819 591

BLAST of WMU64657 vs. TAIR10
Match: AT5G39900.1 (AT5G39900.1 Small GTP-binding protein)

HSP 1 Score: 73.6 bits (179), Expect = 1.1e-13
Identity = 47/133 (35.34%), Postives = 73/133 (54.89%), Query Frame = -3

Query: 1   PSILPEPGKRKSIE--EPFVKIEMLTPKEYIGPLMELAQERRGEFQENENLLQK---LEH 60
           P+ LP   K +     EP V   ++ P EY+G ++ L  +RRG+  E   +  +   L++
Sbjct: 452 PAALPSNPKYRVTASWEPTVIATIILPSEYVGAVINLCSDRRGQQLEYTFIDAQRVFLKY 511

Query: 61  QSPTLCR*PKMVGDFFDQLKSRSKGYASMEYSFIGYRESELIKLDIQINGDRVEPIGKQI 120
           Q P      ++V DF+D+LKS + GYAS +Y    Y+ S+L+KLDI +NG  V+ +   +
Sbjct: 512 QLPLR----EIVVDFYDELKSITSGYASFDYEDAEYQASDLVKLDILLNGQAVDALATIV 571

Query: 121 SRQQDNKAYSVGR 180
            +Q   KAY VG+
Sbjct: 572 HKQ---KAYRVGK 577

Query: 181 240
           
Sbjct: 632 577

Query: 241 300
           
Sbjct: 692 577

Query: 301 360
           
Sbjct: 752 577

Query: 361 385
           
Sbjct: 812 577

BLAST of WMU64657 vs. Swiss-Prot
Match: GUFP_VITVI (Translation factor GUF1 homolog, chloroplastic OS=Vitis vinifera GN=VITISV_013255 PE=3 SV=1)

HSP 1 Score: 177.2 bits (448), Expect = 1.3e-43
Identity = 94/141 (66.67%), Postives = 112/141 (79.43%), Query Frame = -3

Query: 1   NGDTVEWLKIPSILPEPGKRKSIEEPFVKIEMLTPKEYIGPLMELAQERRGEFQENENLL 60
           NGDTVE    PS+LPEPGKR SIEEP+VKIEMLTPK+YIGPLMELAQ+RRGEF+E + + 
Sbjct: 458 NGDTVECSN-PSLLPEPGKRTSIEEPYVKIEMLTPKDYIGPLMELAQDRRGEFKEMKFIT 517

Query: 61  QK---LEHQSPTLCR*PKMVGDFFDQLKSRSKGYASMEYSFIGYRESELIKLDIQINGDR 120
           +    + ++ P      +MVGDFFDQLKSRSKGYASMEYSF+GY+ESELIKLDIQING+R
Sbjct: 518 ENRASITYELPLA----EMVGDFFDQLKSRSKGYASMEYSFLGYKESELIKLDIQINGER 577

Query: 121 VEPIGKQISRQQDNKAYSVGR 180
           VEP+   + +   +KAY+VGR
Sbjct: 578 VEPLATIVHK---DKAYAVGR 590

Query: 181 240
           
Sbjct: 638 590

Query: 241 300
           
Sbjct: 698 590

Query: 301 360
           
Sbjct: 758 590

Query: 361 415
           
Sbjct: 818 590

BLAST of WMU64657 vs. Swiss-Prot
Match: GUFP_RICCO (Translation factor GUF1 homolog, chloroplastic OS=Ricinus communis GN=RCOM_1767360 PE=3 SV=1)

HSP 1 Score: 170.2 bits (430), Expect = 1.6e-41
Identity = 92/141 (65.25%), Postives = 108/141 (76.60%), Query Frame = -3

Query: 1   NGDTVEWLKIPSILPEPGKRKSIEEPFVKIEMLTPKEYIGPLMELAQERRGEFQENE--- 60
           +GDTVE    PS+LPEPGKR+SIEEP VKIEMLTPK+YIGPLMELAQ+RRGEF+E     
Sbjct: 461 DGDTVECSN-PSLLPEPGKRRSIEEPLVKIEMLTPKDYIGPLMELAQDRRGEFKEMRFIT 520

Query: 61  NLLQKLEHQSPTLCR*PKMVGDFFDQLKSRSKGYASMEYSFIGYRESELIKLDIQINGDR 120
           N    + ++ P      +MVGDFFDQLKSRSKGYASMEY+FIGY+ES+LIKL+IQINGD 
Sbjct: 521 NSRASITYELPLA----EMVGDFFDQLKSRSKGYASMEYTFIGYKESDLIKLEIQINGDP 580

Query: 121 VEPIGKQISRQQDNKAYSVGR 180
           VEP+   + +   +KAY VGR
Sbjct: 581 VEPLATIVHK---DKAYPVGR 593

Query: 181 240
           
Sbjct: 641 593

Query: 241 300
           
Sbjct: 701 593

Query: 301 360
           
Sbjct: 761 593

Query: 361 415
           
Sbjct: 821 593

BLAST of WMU64657 vs. Swiss-Prot
Match: GUFP_ORYSI (Translation factor GUF1 homolog, chloroplastic OS=Oryza sativa subsp. indica GN=OsI_05919 PE=3 SV=1)

HSP 1 Score: 169.1 bits (427), Expect = 3.5e-41
Identity = 92/140 (65.71%), Postives = 108/140 (77.14%), Query Frame = -3

Query: 1   NGDTVEWLKIPSILPEPGKRKSIEEPFVKIEMLTPKEYIGPLMELAQERRGEFQENENLL 60
           +G+TVE    PS+LPEPGKR+SIEEP+VKI+MLTPKEYIGP+MEL QERRGEF+E   + 
Sbjct: 414 DGETVECSN-PSLLPEPGKRRSIEEPYVKIDMLTPKEYIGPIMELGQERRGEFKEMNFI- 473

Query: 61  QKLEHQSPTLCR*P--KMVGDFFDQLKSRSKGYASMEYSFIGYRESELIKLDIQINGDRV 120
              E+++  +   P  +MVGDFFDQLKSRSKGYASMEYS IGYRES L+KLDIQINGD V
Sbjct: 474 --TENRASVVYELPLAEMVGDFFDQLKSRSKGYASMEYSLIGYRESNLVKLDIQINGDPV 533

Query: 121 EPIGKQISRQQDNKAYSVGR 180
           E +   + R   +KAYSVGR
Sbjct: 534 EALSTIVHR---DKAYSVGR 546

Query: 181 240
           
Sbjct: 594 546

Query: 241 300
           
Sbjct: 654 546

Query: 301 360
           
Sbjct: 714 546

Query: 361 415
           
Sbjct: 774 546

BLAST of WMU64657 vs. Swiss-Prot
Match: GUFP_ORYSJ (Translation factor GUF1 homolog, chloroplastic OS=Oryza sativa subsp. japonica GN=Os02g0157700 PE=3 SV=1)

HSP 1 Score: 169.1 bits (427), Expect = 3.5e-41
Identity = 92/140 (65.71%), Postives = 108/140 (77.14%), Query Frame = -3

Query: 1   NGDTVEWLKIPSILPEPGKRKSIEEPFVKIEMLTPKEYIGPLMELAQERRGEFQENENLL 60
           +G+TVE    PS+LPEPGKR+SIEEP+VKI+MLTPKEYIGP+MEL QERRGEF+E   + 
Sbjct: 458 DGETVECSN-PSLLPEPGKRRSIEEPYVKIDMLTPKEYIGPIMELGQERRGEFKEMNFI- 517

Query: 61  QKLEHQSPTLCR*P--KMVGDFFDQLKSRSKGYASMEYSFIGYRESELIKLDIQINGDRV 120
              E+++  +   P  +MVGDFFDQLKSRSKGYASMEYS IGYRES L+KLDIQINGD V
Sbjct: 518 --TENRASVVYELPLAEMVGDFFDQLKSRSKGYASMEYSLIGYRESNLVKLDIQINGDPV 577

Query: 121 EPIGKQISRQQDNKAYSVGR 180
           E +   + R   +KAYSVGR
Sbjct: 578 EALSTIVHR---DKAYSVGR 590

Query: 181 240
           
Sbjct: 638 590

Query: 241 300
           
Sbjct: 698 590

Query: 301 360
           
Sbjct: 758 590

Query: 361 415
           
Sbjct: 818 590

BLAST of WMU64657 vs. Swiss-Prot
Match: GUFP_POPTR (Translation factor GUF1 homolog, chloroplastic OS=Populus trichocarpa GN=POPTRDRAFT_815670 PE=3 SV=2)

HSP 1 Score: 164.5 bits (415), Expect = 8.7e-40
Identity = 84/131 (64.12%), Postives = 104/131 (79.39%), Query Frame = -3

Query: 1   PSILPEPGKRKSIEEPFVKIEMLTPKEYIGPLMELAQERRGEFQENENLLQ---KLEHQS 60
           PS+LPEPGKR+S+EEPFVKIE+LTPK+YIG LMELAQERRGEF+E + + +    + ++ 
Sbjct: 465 PSLLPEPGKRRSVEEPFVKIELLTPKDYIGTLMELAQERRGEFKEMKYITENRASITYEL 524

Query: 61  PTLCR*PKMVGDFFDQLKSRSKGYASMEYSFIGYRESELIKLDIQINGDRVEPIGKQISR 120
           P      +MVGDFFDQLKSRSKGYASMEY+ +GY+ES+LI+LDIQINGD VEP+   + +
Sbjct: 525 PL----AEMVGDFFDQLKSRSKGYASMEYTVVGYKESDLIRLDIQINGDPVEPLATIVHK 584

Query: 121 QQDNKAYSVGR 180
              +KAYSVGR
Sbjct: 585 ---DKAYSVGR 588

Query: 181 240
           
Sbjct: 645 588

Query: 241 300
           
Sbjct: 705 588

Query: 301 360
           
Sbjct: 765 588

Query: 361 385
           
Sbjct: 825 588

BLAST of WMU64657 vs. NCBI nr
Match: gi|659069361|ref|XP_008449438.1| (PREDICTED: translation factor GUF1 homolog, chloroplastic isoform X2 [Cucumis melo])

HSP 1 Score: 189.9 bits (481), Expect = 3.1e-45
Identity = 103/141 (73.05%), Postives = 114/141 (80.85%), Query Frame = -3

Query: 1   NGDTVEWLKIPSILPEPGKRKSIEEPFVKIEMLTPKEYIGPLMELAQERRGEFQENENLL 60
           NGDTVE    PS+LPEPGKR+SIEEPFVKIEMLTPKEYIGPLMELAQERRGEFQE + + 
Sbjct: 460 NGDTVECSN-PSLLPEPGKRRSIEEPFVKIEMLTPKEYIGPLMELAQERRGEFQEMKFIT 519

Query: 61  Q---KLEHQSPTLCR*PKMVGDFFDQLKSRSKGYASMEYSFIGYRESELIKLDIQINGDR 120
           +    + +  P      +MVGDFFDQLKSRSKGYASMEYSFIGYRESELIKLDIQINGDR
Sbjct: 520 EVRASITYALPLA----EMVGDFFDQLKSRSKGYASMEYSFIGYRESELIKLDIQINGDR 579

Query: 121 VEPIGKQISRQQDNKAYSVGR 180
           VEP+   +    +NKAYSVGR
Sbjct: 580 VEPLSTIV---HNNKAYSVGR 592

Query: 181 240
           
Sbjct: 640 592

Query: 241 300
           
Sbjct: 700 592

Query: 301 360
           
Sbjct: 760 592

Query: 361 415
           
Sbjct: 820 592

BLAST of WMU64657 vs. NCBI nr
Match: gi|659069359|ref|XP_008449429.1| (PREDICTED: translation factor GUF1 homolog, chloroplastic isoform X1 [Cucumis melo])

HSP 1 Score: 189.9 bits (481), Expect = 3.1e-45
Identity = 103/141 (73.05%), Postives = 114/141 (80.85%), Query Frame = -3

Query: 1   NGDTVEWLKIPSILPEPGKRKSIEEPFVKIEMLTPKEYIGPLMELAQERRGEFQENENLL 60
           NGDTVE    PS+LPEPGKR+SIEEPFVKIEMLTPKEYIGPLMELAQERRGEFQE + + 
Sbjct: 467 NGDTVECSN-PSLLPEPGKRRSIEEPFVKIEMLTPKEYIGPLMELAQERRGEFQEMKFIT 526

Query: 61  Q---KLEHQSPTLCR*PKMVGDFFDQLKSRSKGYASMEYSFIGYRESELIKLDIQINGDR 120
           +    + +  P      +MVGDFFDQLKSRSKGYASMEYSFIGYRESELIKLDIQINGDR
Sbjct: 527 EVRASITYALPLA----EMVGDFFDQLKSRSKGYASMEYSFIGYRESELIKLDIQINGDR 586

Query: 121 VEPIGKQISRQQDNKAYSVGR 180
           VEP+   +    +NKAYSVGR
Sbjct: 587 VEPLSTIV---HNNKAYSVGR 599

Query: 181 240
           
Sbjct: 647 599

Query: 241 300
           
Sbjct: 707 599

Query: 301 360
           
Sbjct: 767 599

Query: 361 415
           
Sbjct: 827 599

BLAST of WMU64657 vs. NCBI nr
Match: gi|778694762|ref|XP_011653860.1| (PREDICTED: translation factor GUF1 homolog, chloroplastic [Cucumis sativus])

HSP 1 Score: 188.0 bits (476), Expect = 1.2e-44
Identity = 102/141 (72.34%), Postives = 113/141 (80.14%), Query Frame = -3

Query: 1   NGDTVEWLKIPSILPEPGKRKSIEEPFVKIEMLTPKEYIGPLMELAQERRGEFQENENLL 60
           NGDTVE    PS+LPEPGKR+SIEEPFVKIEMLTPKEYIGPLMELAQERRGEFQE + + 
Sbjct: 460 NGDTVECSN-PSLLPEPGKRRSIEEPFVKIEMLTPKEYIGPLMELAQERRGEFQEMKFIT 519

Query: 61  Q---KLEHQSPTLCR*PKMVGDFFDQLKSRSKGYASMEYSFIGYRESELIKLDIQINGDR 120
           +    + +  P      +MVGDFFDQLKSRSKGYASMEYSF GYRESELIKLDIQINGDR
Sbjct: 520 EVRASITYALPLA----EMVGDFFDQLKSRSKGYASMEYSFTGYRESELIKLDIQINGDR 579

Query: 121 VEPIGKQISRQQDNKAYSVGR 180
           VEP+   +    +NKAYSVGR
Sbjct: 580 VEPLSTIV---HNNKAYSVGR 592

Query: 181 240
           
Sbjct: 640 592

Query: 241 300
           
Sbjct: 700 592

Query: 301 360
           
Sbjct: 760 592

Query: 361 415
           
Sbjct: 820 592

BLAST of WMU64657 vs. NCBI nr
Match: gi|700199572|gb|KGN54730.1| (hypothetical protein Csa_4G437010 [Cucumis sativus])

HSP 1 Score: 188.0 bits (476), Expect = 1.2e-44
Identity = 102/141 (72.34%), Postives = 113/141 (80.14%), Query Frame = -3

Query: 1   NGDTVEWLKIPSILPEPGKRKSIEEPFVKIEMLTPKEYIGPLMELAQERRGEFQENENLL 60
           NGDTVE    PS+LPEPGKR+SIEEPFVKIEMLTPKEYIGPLMELAQERRGEFQE + + 
Sbjct: 153 NGDTVECSN-PSLLPEPGKRRSIEEPFVKIEMLTPKEYIGPLMELAQERRGEFQEMKFIT 212

Query: 61  Q---KLEHQSPTLCR*PKMVGDFFDQLKSRSKGYASMEYSFIGYRESELIKLDIQINGDR 120
           +    + +  P      +MVGDFFDQLKSRSKGYASMEYSF GYRESELIKLDIQINGDR
Sbjct: 213 EVRASITYALPLA----EMVGDFFDQLKSRSKGYASMEYSFTGYRESELIKLDIQINGDR 272

Query: 121 VEPIGKQISRQQDNKAYSVGR 180
           VEP+   +    +NKAYSVGR
Sbjct: 273 VEPLSTIV---HNNKAYSVGR 285

Query: 181 240
           
Sbjct: 333 285

Query: 241 300
           
Sbjct: 393 285

Query: 301 360
           
Sbjct: 453 285

Query: 361 415
           
Sbjct: 513 285

BLAST of WMU64657 vs. NCBI nr
Match: gi|720041750|ref|XP_010269009.1| (PREDICTED: translation factor GUF1 homolog, chloroplastic [Nelumbo nucifera])

HSP 1 Score: 181.8 bits (460), Expect = 8.4e-43
Identity = 96/141 (68.09%), Postives = 114/141 (80.85%), Query Frame = -3

Query: 1   NGDTVEWLKIPSILPEPGKRKSIEEPFVKIEMLTPKEYIGPLMELAQERRGEFQENENLL 60
           NGDTVE    PS+LPEPGKR+SIEEPFVKIEMLTPK+YIGPLMELAQ+RRGEF+E + + 
Sbjct: 464 NGDTVECSN-PSLLPEPGKRRSIEEPFVKIEMLTPKDYIGPLMELAQDRRGEFKEMKYIT 523

Query: 61  QK---LEHQSPTLCR*PKMVGDFFDQLKSRSKGYASMEYSFIGYRESELIKLDIQINGDR 120
           +    + ++ P      +MVGDFFDQLKSRSKGYASMEYSF+GYRESELIKLDIQINGD 
Sbjct: 524 ENRASITYELPL----SEMVGDFFDQLKSRSKGYASMEYSFVGYRESELIKLDIQINGDP 583

Query: 121 VEPIGKQISRQQDNKAYSVGR 180
           VEP+   + +   +KAY+VGR
Sbjct: 584 VEPLATIVHK---DKAYAVGR 596

Query: 181 240
           
Sbjct: 644 596

Query: 241 300
           
Sbjct: 704 596

Query: 301 360
           
Sbjct: 764 596

Query: 361 415
           
Sbjct: 824 596

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT5G08650.17.1e-4063.57 Small GTP-binding protein[more]
AT5G39900.11.1e-1335.34 Small GTP-binding protein[more]
Match NameE-valueIdentityDescription
GUFP_VITVI1.3e-4366.67Translation factor GUF1 homolog, chloroplastic OS=Vitis vinifera GN=VITISV_01325... [more]
GUFP_RICCO1.6e-4165.25Translation factor GUF1 homolog, chloroplastic OS=Ricinus communis GN=RCOM_17673... [more]
GUFP_ORYSI3.5e-4165.71Translation factor GUF1 homolog, chloroplastic OS=Oryza sativa subsp. indica GN=... [more]
GUFP_ORYSJ3.5e-4165.71Translation factor GUF1 homolog, chloroplastic OS=Oryza sativa subsp. japonica G... [more]
GUFP_POPTR8.7e-4064.12Translation factor GUF1 homolog, chloroplastic OS=Populus trichocarpa GN=POPTRDR... [more]
Match NameE-valueIdentityDescription
gi|659069361|ref|XP_008449438.1|3.1e-4573.05PREDICTED: translation factor GUF1 homolog, chloroplastic isoform X2 [Cucumis me... [more]
gi|659069359|ref|XP_008449429.1|3.1e-4573.05PREDICTED: translation factor GUF1 homolog, chloroplastic isoform X1 [Cucumis me... [more]
gi|778694762|ref|XP_011653860.1|1.2e-4472.34PREDICTED: translation factor GUF1 homolog, chloroplastic [Cucumis sativus][more]
gi|700199572|gb|KGN54730.1|1.2e-4472.34hypothetical protein Csa_4G437010 [Cucumis sativus][more]
gi|720041750|ref|XP_010269009.1|8.4e-4368.09PREDICTED: translation factor GUF1 homolog, chloroplastic [Nelumbo nucifera][more]
The following terms have been associated with this transcribed_cluster:
Vocabulary: INTERPRO
TermDefinition
IPR000640EFG_V-like
IPR009022EFG_III

This transcribed_cluster is associated with the following gene feature(s):

Feature NameUnique NameType
Cla006097Cla006097gene
Cla006098Cla006098gene


The following EST feature(s) are a part of this transcribed_cluster:

Feature NameUnique NameType
S2_0113149S2_0113149EST


Analysis Name: InterPro Annotations of watermelon unigene v2
Date Performed: 2016-11-16
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000640Translation elongation factor EFG, V domainGENE3DG3DSA:3.30.70.240coord: 4..40
score: 7.
IPR000640Translation elongation factor EFG, V domainPFAMPF00679EFG_Ccoord: 4..35
score: 3.
IPR009022Elongation factor G, III-V domainunknownSSF54980EF-G C-terminal domain-likecoord: 4..44
score: 7.6
NoneNo IPR availablePANTHERPTHR23115TRANSLATION FACTORcoord: 1..46
score: 1.6
NoneNo IPR availablePANTHERPTHR23115:SF189TRANSLATION FACTOR GUF1 HOMOLOG, CHLOROPLASTICcoord: 1..46
score: 1.6