WMU44513 (transcribed_cluster) Watermelon (97103) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	WMU44513
Type	transcribed_cluster
Organism	Citrullus. lanatus (Watermelon (97103) v1)
Description	DNA-binding WRKY
Location	Chr5 : 117737 .. 118755 (+)
Sequence length	331

Genome Viewer

Sequences

The following sequences are available for this feature:

transcribed_cluster sequence

CCAAAAAGTCGTCAAGAACAGCCTTCATCCTAGAAGCTACTATCGTTGCACGCATAGCAACTGCCGAGTGAAGAAGAGAGTGGAGAGACTGTCGGAGGATTGCCGGATGGTGATAACCACCTACGAAGGTAGACACAACCACTCTCCTTGCGACGACTCCAATTCTTCAGAGCACGAACCCTTTACATCCTTCTGACTTCCATTTTTATTTTATATTTCTTTTCTTATATCCTGCTGCCATCCTAATTTCATTTCCCCCTTCCTTTCCTTTCCTTAATTCCTTTGCTTGTATAATAATAATAATTATTATTATTATTATTTCTTTAACTTT

Library


Library category	ESTs
watermelon fruit 26d library	2

Homology

[Close]

BLAST of WMU44513 vs. TAIR10
Match: AT2G44745.1 (AT2G44745.1 WRKY family transcription factor)

HSP 1 Score: 120.2 bits (300), Expect = 8.2e-28
Identity = 54/64 (84.38%), Postives = 56/64 (87.50%), Query Frame = 2


Query: 2   QKVVKNSLHPRSYYRCTHSNCRVKKRVERLSEDCRMVITTYEGRHNHSPCDDSNSSEHEP 61
           QKVVKNSLHPRSYYRCTH+NCRVKKRVERLSEDCRMVITTYEGRHNH P DDS S +H+ 
Sbjct: 155 QKVVKNSLHPRSYYRCTHNNCRVKKRVERLSEDCRMVITTYEGRHNHIPSDDSTSPDHDC 214

Query: 62  FTSF 121
            +SF
Sbjct: 215 LSSF 218

Query: 122  181
           
Sbjct: 275  218

Query: 182  194
           
Sbjct: 335  218

[Close]

BLAST of WMU44513 vs. TAIR10
Match: AT4G39410.1 (AT4G39410.1 WRKY DNA-binding protein 13)

HSP 1 Score: 92.4 bits (228), Expect = 1.8e-19
Identity = 47/70 (67.14%), Postives = 52/70 (74.29%), Query Frame = 2


Query: 2   QKVVKNSLHPRSYYRCTHSNCRVKKRVERLSEDCRMVITTYEGRHNHSPC----DDSNSS 61
           QKVVKN+ HPRSYYRCT   CRVKKRVERL++D RMVITTYEGRH HSP     DDS S+
Sbjct: 233 QKVVKNTQHPRSYYRCTQDKCRVKKRVERLADDPRMVITTYEGRHLHSPSNHLDDDSLST 292

Query: 62  E--HEPFTSF 121
              H P ++F
Sbjct: 293 SHLHPPLSNF 302

Query: 122  181
           
Sbjct: 353  302

Query: 182  194
           
Sbjct: 413  302

[Close]

BLAST of WMU44513 vs. TAIR10
Match: AT1G64000.1 (AT1G64000.1 WRKY DNA-binding protein 56)

HSP 1 Score: 76.3 bits (186), Expect = 1.4e-14
Identity = 33/51 (64.71%), Postives = 41/51 (80.39%), Query Frame = 2


Query: 2   QKVVKNSLHPRSYYRCTHSNCRVKKRVERLSEDCRMVITTYEGRHNHSPCD 61
           QK VKN+ HPRSYYRCT+  C VKK+V+RL++D  +V+TTYEG HNH PC+
Sbjct: 124 QKSVKNNAHPRSYYRCTYHTCNVKKQVQRLAKDPNVVVTTYEGVHNH-PCE 173

Query: 62   121
           
Sbjct: 184  173

Query: 122  155
           
Sbjct: 244  173

[Close]

BLAST of WMU44513 vs. TAIR10
Match: AT2G37260.1 (AT2G37260.1 WRKY family transcription factor family protein)

HSP 1 Score: 75.1 bits (183), Expect = 3.0e-14
Identity = 36/63 (57.14%), Postives = 40/63 (63.49%), Query Frame = 2


Query: 2   QKVVKNSLHPRSYYRCTHSNCRVKKRVERLSEDCRMVITTYEGRHNHSPCDDSNSSEHEP 61
           QKVV  + +PRSYYRCT +NCR +K VER S+D R  ITTYEG+HNH       SS   P
Sbjct: 359 QKVVGGNAYPRSYYRCTSANCRARKHVERASDDPRAFITTYEGKHNHHLLLSPPSSSTLP 418

Query: 62  FTS 121
           F S
Sbjct: 419 FNS 421

Query: 122  181
           
Sbjct: 479  421

Query: 182  191
           
Sbjct: 539  421

HSP 2 Score: 55.1 bits (131), Expect = 3.2e-08
Identity = 27/48 (56.25%), Postives = 31/48 (64.58%), Query Frame = 2


Query: 2   QKVVKNSLHPRSYYRCTHSNCRVKKRVERLSEDCRMVITTYEGRHNHS 61
           QK VK S  PRSYY+CTH  C VKK+VER S + ++    Y+G HNHS
Sbjct: 175 QKQVKGSECPRSYYKCTHPKCPVKKKVER-SVEGQVSEIVYQGEHNHS 221

Query: 62   121
           
Sbjct: 235  221

Query: 122  146
           
Sbjct: 295  221

[Close]

BLAST of WMU44513 vs. TAIR10
Match: AT5G41570.1 (AT5G41570.1 WRKY DNA-binding protein 24)

HSP 1 Score: 74.3 bits (181), Expect = 5.1e-14
Identity = 32/51 (62.75%), Postives = 41/51 (80.39%), Query Frame = 2


Query: 2   QKVVKNSLHPRSYYRCTHSNCRVKKRVERLSEDCRMVITTYEGRHNHSPCD 61
           QK VK++ HPRSYYRCT+  C VKK+V+RL++D  +V+TTYEG HNH PC+
Sbjct: 108 QKSVKHNAHPRSYYRCTYHTCNVKKQVQRLAKDPNVVVTTYEGVHNH-PCE 157

Query: 62   121
           
Sbjct: 168  157

Query: 122  155
           
Sbjct: 228  157

[Close]

BLAST of WMU44513 vs. Swiss-Prot
Match: WRK12_ARATH (Probable WRKY transcription factor 12 OS=Arabidopsis thaliana GN=WRKY12 PE=2 SV=1)

HSP 1 Score: 120.2 bits (300), Expect = 1.5e-26
Identity = 54/64 (84.38%), Postives = 56/64 (87.50%), Query Frame = 2


Query: 2   QKVVKNSLHPRSYYRCTHSNCRVKKRVERLSEDCRMVITTYEGRHNHSPCDDSNSSEHEP 61
           QKVVKNSLHPRSYYRCTH+NCRVKKRVERLSEDCRMVITTYEGRHNH P DDS S +H+ 
Sbjct: 155 QKVVKNSLHPRSYYRCTHNNCRVKKRVERLSEDCRMVITTYEGRHNHIPSDDSTSPDHDC 214

Query: 62  FTSF 121
            +SF
Sbjct: 215 LSSF 218

Query: 122  181
           
Sbjct: 275  218

Query: 182  194
           
Sbjct: 335  218

[Close]

BLAST of WMU44513 vs. Swiss-Prot
Match: WRK13_ARATH (Probable WRKY transcription factor 13 OS=Arabidopsis thaliana GN=WRKY13 PE=2 SV=1)

HSP 1 Score: 92.4 bits (228), Expect = 3.2e-18
Identity = 47/70 (67.14%), Postives = 52/70 (74.29%), Query Frame = 2


Query: 2   QKVVKNSLHPRSYYRCTHSNCRVKKRVERLSEDCRMVITTYEGRHNHSPC----DDSNSS 61
           QKVVKN+ HPRSYYRCT   CRVKKRVERL++D RMVITTYEGRH HSP     DDS S+
Sbjct: 233 QKVVKNTQHPRSYYRCTQDKCRVKKRVERLADDPRMVITTYEGRHLHSPSNHLDDDSLST 292

Query: 62  E--HEPFTSF 121
              H P ++F
Sbjct: 293 SHLHPPLSNF 302

Query: 122  181
           
Sbjct: 353  302

Query: 182  194
           
Sbjct: 413  302

[Close]

BLAST of WMU44513 vs. Swiss-Prot
Match: WRK56_ARATH (Probable WRKY transcription factor 56 OS=Arabidopsis thaliana GN=WRKY56 PE=2 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 2.4e-13
Identity = 33/51 (64.71%), Postives = 41/51 (80.39%), Query Frame = 2


Query: 2   QKVVKNSLHPRSYYRCTHSNCRVKKRVERLSEDCRMVITTYEGRHNHSPCD 61
           QK VKN+ HPRSYYRCT+  C VKK+V+RL++D  +V+TTYEG HNH PC+
Sbjct: 124 QKSVKNNAHPRSYYRCTYHTCNVKKQVQRLAKDPNVVVTTYEGVHNH-PCE 173

Query: 62   121
           
Sbjct: 184  173

Query: 122  155
           
Sbjct: 244  173

[Close]

BLAST of WMU44513 vs. Swiss-Prot
Match: WRK44_ARATH (WRKY transcription factor 44 OS=Arabidopsis thaliana GN=WRKY44 PE=1 SV=2)

HSP 1 Score: 75.1 bits (183), Expect = 5.4e-13
Identity = 36/63 (57.14%), Postives = 40/63 (63.49%), Query Frame = 2


Query: 2   QKVVKNSLHPRSYYRCTHSNCRVKKRVERLSEDCRMVITTYEGRHNHSPCDDSNSSEHEP 61
           QKVV  + +PRSYYRCT +NCR +K VER S+D R  ITTYEG+HNH       SS   P
Sbjct: 359 QKVVGGNAYPRSYYRCTSANCRARKHVERASDDPRAFITTYEGKHNHHLLLSPPSSSTLP 418

Query: 62  FTS 121
           F S
Sbjct: 419 FNS 421

Query: 122  181
           
Sbjct: 479  421

Query: 182  191
           
Sbjct: 539  421

HSP 2 Score: 55.1 bits (131), Expect = 5.7e-07
Identity = 27/48 (56.25%), Postives = 31/48 (64.58%), Query Frame = 2


Query: 2   QKVVKNSLHPRSYYRCTHSNCRVKKRVERLSEDCRMVITTYEGRHNHS 61
           QK VK S  PRSYY+CTH  C VKK+VER S + ++    Y+G HNHS
Sbjct: 175 QKQVKGSECPRSYYKCTHPKCPVKKKVER-SVEGQVSEIVYQGEHNHS 221

Query: 62   121
           
Sbjct: 235  221

Query: 122  146
           
Sbjct: 295  221

[Close]

BLAST of WMU44513 vs. Swiss-Prot
Match: WRK24_ARATH (Probable WRKY transcription factor 24 OS=Arabidopsis thaliana GN=WRKY24 PE=2 SV=1)

HSP 1 Score: 74.3 bits (181), Expect = 9.1e-13
Identity = 32/51 (62.75%), Postives = 41/51 (80.39%), Query Frame = 2


Query: 2   QKVVKNSLHPRSYYRCTHSNCRVKKRVERLSEDCRMVITTYEGRHNHSPCD 61
           QK VK++ HPRSYYRCT+  C VKK+V+RL++D  +V+TTYEG HNH PC+
Sbjct: 108 QKSVKHNAHPRSYYRCTYHTCNVKKQVQRLAKDPNVVVTTYEGVHNH-PCE 157

Query: 62   121
           
Sbjct: 168  157

Query: 122  155
           
Sbjct: 228  157

[Close]

BLAST of WMU44513 vs. NCBI nr
Match: gi|659074721|ref|XP_008437760.1| (PREDICTED: probable WRKY transcription factor 12 [Cucumis melo])

HSP 1 Score: 140.6 bits (353), Expect = 1.7e-30
Identity = 64/64 (100.00%), Postives = 64/64 (100.00%), Query Frame = 2


Query: 2   QKVVKNSLHPRSYYRCTHSNCRVKKRVERLSEDCRMVITTYEGRHNHSPCDDSNSSEHEP 61
           QKVVKNSLHPRSYYRCTHSNCRVKKRVERLSEDCRMVITTYEGRHNHSPCDDSNSSEHEP
Sbjct: 157 QKVVKNSLHPRSYYRCTHSNCRVKKRVERLSEDCRMVITTYEGRHNHSPCDDSNSSEHEP 216

Query: 62  FTSF 121
           FTSF
Sbjct: 217 FTSF 220

Query: 122  181
           
Sbjct: 277  220

Query: 182  194
           
Sbjct: 337  220

[Close]

BLAST of WMU44513 vs. NCBI nr
Match: gi|449431940|ref|XP_004133758.1| (PREDICTED: probable WRKY transcription factor 12 [Cucumis sativus])

HSP 1 Score: 139.4 bits (350), Expect = 3.7e-30
Identity = 63/64 (98.44%), Postives = 64/64 (100.00%), Query Frame = 2


Query: 2   QKVVKNSLHPRSYYRCTHSNCRVKKRVERLSEDCRMVITTYEGRHNHSPCDDSNSSEHEP 61
           QKVVKNSLHPRSYYRCTHSNCRVKKRVERLSEDCRMVITTYEGRHNH+PCDDSNSSEHEP
Sbjct: 156 QKVVKNSLHPRSYYRCTHSNCRVKKRVERLSEDCRMVITTYEGRHNHTPCDDSNSSEHEP 215

Query: 62  FTSF 121
           FTSF
Sbjct: 216 FTSF 219

Query: 122  181
           
Sbjct: 276  219

Query: 182  194
           
Sbjct: 336  219

[Close]

BLAST of WMU44513 vs. NCBI nr
Match: gi|1000951459|ref|XP_015579263.1| (PREDICTED: probable WRKY transcription factor 12 [Ricinus communis])

HSP 1 Score: 136.7 bits (343), Expect = 2.4e-29
Identity = 63/64 (98.44%), Postives = 63/64 (98.44%), Query Frame = 2


Query: 2   QKVVKNSLHPRSYYRCTHSNCRVKKRVERLSEDCRMVITTYEGRHNHSPCDDSNSSEHEP 61
           QKVVKNSLHPRSYYRCTHSNCRVKKRVERLSEDCRMVITTYEGRHNHSPCDDSNSSEHE 
Sbjct: 142 QKVVKNSLHPRSYYRCTHSNCRVKKRVERLSEDCRMVITTYEGRHNHSPCDDSNSSEHEC 201

Query: 62  FTSF 121
           FTSF
Sbjct: 202 FTSF 205

Query: 122  181
           
Sbjct: 262  205

Query: 182  194
           
Sbjct: 322  205

[Close]

BLAST of WMU44513 vs. NCBI nr
Match: gi|802700455|ref|XP_012083720.1| (PREDICTED: probable WRKY transcription factor 12 [Jatropha curcas])

HSP 1 Score: 136.7 bits (343), Expect = 2.4e-29
Identity = 63/64 (98.44%), Postives = 63/64 (98.44%), Query Frame = 2


Query: 2   QKVVKNSLHPRSYYRCTHSNCRVKKRVERLSEDCRMVITTYEGRHNHSPCDDSNSSEHEP 61
           QKVVKNSLHPRSYYRCTHSNCRVKKRVERLSEDCRMVITTYEGRHNHSPCDDSNSSEHE 
Sbjct: 144 QKVVKNSLHPRSYYRCTHSNCRVKKRVERLSEDCRMVITTYEGRHNHSPCDDSNSSEHEC 203

Query: 62  FTSF 121
           FTSF
Sbjct: 204 FTSF 207

Query: 122  181
           
Sbjct: 264  207

Query: 182  194
           
Sbjct: 324  207

[Close]

BLAST of WMU44513 vs. NCBI nr
Match: gi|976914321|gb|KVI00540.1| (DNA-binding WRKY, partial [Cynara cardunculus var. scolymus])

HSP 1 Score: 135.6 bits (340), Expect = 5.3e-29
Identity = 62/64 (96.88%), Postives = 63/64 (98.44%), Query Frame = 2


Query: 2   QKVVKNSLHPRSYYRCTHSNCRVKKRVERLSEDCRMVITTYEGRHNHSPCDDSNSSEHEP 61
           QKVVKNSLHPRSYYRCTH+NCRVKKRVERLSEDCRMVITTYEGRHNHSPCDDSNSSEHE 
Sbjct: 228 QKVVKNSLHPRSYYRCTHTNCRVKKRVERLSEDCRMVITTYEGRHNHSPCDDSNSSEHEC 287

Query: 62  FTSF 121
           FTSF
Sbjct: 288 FTSF 291

Query: 122  181
           
Sbjct: 348  291

Query: 182  194
           
Sbjct: 408  291

The following BLAST results are available for this feature:

BLAST of WMU44513 vs. TAIR10


Match Name	E-value	Identity	Description
AT2G44745.1	8.2e-28	84.38	WRKY family transcription factor	[more]
AT4G39410.1	1.8e-19	67.14	WRKY DNA-binding protein 13	[more]
AT1G64000.1	1.4e-14	64.71	WRKY DNA-binding protein 56	[more]
AT2G37260.1	3.0e-14	57.14	WRKY family transcription factor family protein	[more]
AT5G41570.1	5.1e-14	62.75	WRKY DNA-binding protein 24	[more]

BLAST of WMU44513 vs. Swiss-Prot


Match Name	E-value	Identity	Description
WRK12_ARATH	1.5e-26	84.38	Probable WRKY transcription factor 12 OS=Arabidopsis thaliana GN=WRKY12 PE=2 SV=...	[more]
WRK13_ARATH	3.2e-18	67.14	Probable WRKY transcription factor 13 OS=Arabidopsis thaliana GN=WRKY13 PE=2 SV=...	[more]
WRK56_ARATH	2.4e-13	64.71	Probable WRKY transcription factor 56 OS=Arabidopsis thaliana GN=WRKY56 PE=2 SV=...	[more]
WRK44_ARATH	5.4e-13	57.14	WRKY transcription factor 44 OS=Arabidopsis thaliana GN=WRKY44 PE=1 SV=2	[more]
WRK24_ARATH	9.1e-13	62.75	Probable WRKY transcription factor 24 OS=Arabidopsis thaliana GN=WRKY24 PE=2 SV=...	[more]

BLAST of WMU44513 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659074721\|ref\|XP_008437760.1\|	1.7e-30	100.00	PREDICTED: probable WRKY transcription factor 12 [Cucumis melo]	[more]
gi\|449431940\|ref\|XP_004133758.1\|	3.7e-30	98.44	PREDICTED: probable WRKY transcription factor 12 [Cucumis sativus]	[more]
gi\|1000951459\|ref\|XP_015579263.1\|	2.4e-29	98.44	PREDICTED: probable WRKY transcription factor 12 [Ricinus communis]	[more]
gi\|802700455\|ref\|XP_012083720.1\|	2.4e-29	98.44	PREDICTED: probable WRKY transcription factor 12 [Jatropha curcas]	[more]
gi\|976914321\|gb\|KVI00540.1\|	5.3e-29	96.88	DNA-binding WRKY, partial [Cynara cardunculus var. scolymus]	[more]

Annotated Terms

The following terms have been associated with this transcribed_cluster:

Vocabulary: **INTERPRO**
Term	Definition
IPR003657	WRKY_dom

Vocabulary: **Molecular Function**
Term	Definition
GO:0003700	transcription factor activity, sequence-specific DNA binding
GO:0043565	sequence-specific DNA binding

Vocabulary: **Biological Process**
Term	Definition
GO:0006355	regulation of transcription, DNA-templated

GO Annotation

GO Assignments

This transcribed_cluster is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006355	regulation of transcription, DNA-templated
molecular_function	GO:0043565	sequence-specific DNA binding
molecular_function	GO:0003700	transcription factor activity, sequence-specific DNA binding

Relationships

This transcribed_cluster is associated with the following gene feature(s):


Feature Name	Unique Name	Type
Cla021067	Cla021067	gene

The following EST feature(s) are a part of this transcribed_cluster:


Feature Name	Unique Name	Type
S3_0073264	S3_0073264	EST
S3_0083898	S3_0083898	EST

InterPro

Analysis Name: InterPro Annotations of watermelon unigene v2
Date Performed: 2016-11-16


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003657	WRKY domain	GENE3D	G3DSA:2.20.25.80		coord: 2..49 score: 6.1
IPR003657	WRKY domain	PFAM	PF03106	WRKY	coord: 2..48 score: 1.6
IPR003657	WRKY domain	SMART	SM00774	WRKY_cls	coord: 2..50 score: 1.1
IPR003657	WRKY domain	PROFILE	PS50811	WRKY	coord: 1..48 score: 20
IPR003657	WRKY domain	unknown	SSF118290	WRKY DNA-binding domain	coord: 2..49 score: 5.75
None	No IPR available	PANTHER	PTHR31221	FAMILY NOT NAMED	coord: 2..57 score: 1.0