WMU43923 (transcribed_cluster) Watermelon (97103) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	WMU43923
Type	transcribed_cluster
Organism	Citrullus. lanatus (Watermelon (97103) v1)
Description	Rapid alkalinization factor
Location	Chr5 : 5793561 .. 5793976 (-)
Sequence length	414

Genome Viewer

Sequences

The following sequences are available for this feature:

transcribed_cluster sequence

ACTGATCCCCAAAATGCAAAGCTCAAACATCCATGGCTTCCTCATTATCTGCGCAATCTCCGCCTCCTATCTGGCCCTCTCTTCATCCACCGCCGTCGATTTTACCGGAGACCGCCAGTTCCTCCACCTTCCGCCGGAATCTGTTTGTTGTTCAGGATCGGGATCGATCGCCGAGTGCCAGTTGGCGGCGGAGTTCGAGATACCGTCGGAGATCAGCCGCCGGATTCTGGCGACTACGAGGCGTTATATTGGCTACAGTGCGATGCGGAGGAACTCGGTGCCGTGCTCCCGACGCGGCGCGTCCTATTACAATTGCCAGGCTGGAGCTCCGGCGAATCCTTACTCTCGTGGGTGCAGCGCCATTACTCGATGCAGGTGATGATTTTGGTGGTTTTCTTTTTCTTTGTAAAGTTT

Library


Library category	ESTs
watermelon fruit 26d library	2

Homology

[Close]

BLAST of WMU43923 vs. TAIR10
Match: AT4G15800.1 (AT4G15800.1 ralf-like 33)

HSP 1 Score: 116.7 bits (291), Expect = 1.1e-26
Identity = 60/86 (69.77%), Postives = 68/86 (79.07%), Query Frame = 2


Query: 128 LPPESVCCSGSGSIAECQLAA---EFEIPSEISRRILATTRRYIGYSAMRRNSVPCSRRG 187
           +P ES C   +G+IAEC L+    EFE+ SEI+RRILATT+ YI Y A+RRN+VPCSRRG
Sbjct: 34  VPIESKC---NGTIAECSLSTAEEEFEMDSEINRRILATTK-YISYGALRRNTVPCSRRG 93

Query: 188 ASYYNCQAGAPANPYSRGCSAITRCR 247
           ASYYNC+ GA ANPYSRGCSAITRCR
Sbjct: 94  ASYYNCRRGAQANPYSRGCSAITRCR 115

Query: 248  307
           
Sbjct: 154  115

Query: 308  367
           
Sbjct: 214  115

Query: 368  377
           
Sbjct: 274  115

[Close]

BLAST of WMU43923 vs. TAIR10
Match: AT3G05490.1 (AT3G05490.1 ralf-like 22)

HSP 1 Score: 112.1 bits (279), Expect = 2.8e-25
Identity = 62/101 (61.39%), Postives = 68/101 (67.33%), Query Frame = 2


Query: 89  TAVDFTGD----RQFLHLPPESVCCSGSGSIAECQLAAE-FEIPSEISRRILATTRRYIG 148
           +AV+ TGD      F+     S+    +GSIAEC    E  E  S+ISRRILA  ++YI 
Sbjct: 19  SAVESTGDFGDSLDFVRAGSSSLFSGCTGSIAECIAEEEEMEFDSDISRRILAQ-KKYIS 78

Query: 149 YSAMRRNSVPCSRRGASYYNCQAGAPANPYSRGCSAITRCR 208
           Y AMRRNSVPCSRRGASYYNCQ GA ANPYSRGCS ITRCR
Sbjct: 79  YGAMRRNSVPCSRRGASYYNCQRGAQANPYSRGCSTITRCR 118

Query: 209  268
           
Sbjct: 139  118

Query: 269  328
           
Sbjct: 199  118

Query: 329  377
           
Sbjct: 259  118

[Close]

BLAST of WMU43923 vs. TAIR10
Match: AT3G16570.1 (AT3G16570.1 rapid alkalinization factor 23)

HSP 1 Score: 112.1 bits (279), Expect = 2.8e-25
Identity = 62/112 (55.36%), Postives = 71/112 (63.39%), Query Frame = 2


Query: 92  AVDFTGDRQFLHLPPESVCCSGSGSIAECQLAA-----------------EFEIPSEISR 151
           + +F GD      PP    C G+  IAEC ++A                 EFE+ SEI+R
Sbjct: 33  STEFAGD-----FPPFETECRGT--IAECSVSAALGDGGDLFYGGGEMGEEFEMDSEINR 92

Query: 152 RILATTRRYIGYSAMRRNSVPCSRRGASYYNCQAGAPANPYSRGCSAITRCR 211
           RILAT RRYI Y A+RRN++PCSRRGASYYNC+ GA ANPYSRGCSAITRCR
Sbjct: 93  RILAT-RRYISYGALRRNTIPCSRRGASYYNCRRGAQANPYSRGCSAITRCR 136

Query: 212  271
           
Sbjct: 153  136

Query: 272  331
           
Sbjct: 213  136

Query: 332  377
           
Sbjct: 273  136

[Close]

BLAST of WMU43923 vs. TAIR10
Match: AT1G02900.1 (AT1G02900.1 rapid alkalinization factor 1)

HSP 1 Score: 109.4 bits (272), Expect = 1.8e-24
Identity = 54/72 (75.00%), Postives = 58/72 (80.56%), Query Frame = 2


Query: 161 GSIAECQLAAEFEIPSEISRRILATTRRYIGYSAMRRNSVPCSRRGASYYNCQAGAPANP 220
           GSIAEC  A E E+ SEI+RRILATT+ YI Y +++RNSVPCSRRGASYYNCQ GA ANP
Sbjct: 49  GSIAECIGAEEEEMDSEINRRILATTK-YISYQSLKRNSVPCSRRGASYYNCQNGAQANP 108

Query: 221 YSRGCSAITRCR 280
           YSRGCS I RCR
Sbjct: 109 YSRGCSKIARCR 119

Query: 281  340
           
Sbjct: 169  119

Query: 341  377
           
Sbjct: 229  119

[Close]

BLAST of WMU43923 vs. TAIR10
Match: AT1G28270.1 (AT1G28270.1 ralf-like 4)

HSP 1 Score: 85.5 bits (210), Expect = 2.8e-17
Identity = 43/81 (53.09%), Postives = 50/81 (61.73%), Query Frame = 2


Query: 131 PPESVCCSGSGSIAECQLAAEFEIPSEISRRILATTRRYIGYSAMRRNSVPCSRRGASYY 190
           P    C +G G I E     E  + SE +RR LA  RRYIGY A+++N+VPCSRRG SYY
Sbjct: 26  PLTKSCINGQGCIGEDD-ELESLMDSETNRRQLARGRRYIGYDALKKNNVPCSRRGRSYY 85

Query: 191 NCQAGAPANPYSRGCSAITRC 250
           +C+     NPY RGCSAIT C
Sbjct: 86  DCKKRRRNNPYRRGCSAITHC 105

Query: 251  310
           
Sbjct: 146  105

Query: 311  370
           
Sbjct: 206  105

Query: 371  374
           
Sbjct: 266  105

[Close]

BLAST of WMU43923 vs. Swiss-Prot
Match: RLF33_ARATH (Protein RALF-like 33 OS=Arabidopsis thaliana GN=RALFL33 PE=2 SV=1)

HSP 1 Score: 116.7 bits (291), Expect = 2.0e-25
Identity = 60/86 (69.77%), Postives = 68/86 (79.07%), Query Frame = 2


Query: 128 LPPESVCCSGSGSIAECQLAA---EFEIPSEISRRILATTRRYIGYSAMRRNSVPCSRRG 187
           +P ES C   +G+IAEC L+    EFE+ SEI+RRILATT+ YI Y A+RRN+VPCSRRG
Sbjct: 34  VPIESKC---NGTIAECSLSTAEEEFEMDSEINRRILATTK-YISYGALRRNTVPCSRRG 93

Query: 188 ASYYNCQAGAPANPYSRGCSAITRCR 247
           ASYYNC+ GA ANPYSRGCSAITRCR
Sbjct: 94  ASYYNCRRGAQANPYSRGCSAITRCR 115

Query: 248  307
           
Sbjct: 154  115

Query: 308  367
           
Sbjct: 214  115

Query: 368  377
           
Sbjct: 274  115

[Close]

BLAST of WMU43923 vs. Swiss-Prot
Match: RLF22_ARATH (Protein RALF-like 22 OS=Arabidopsis thaliana GN=RALFL22 PE=3 SV=1)

HSP 1 Score: 112.1 bits (279), Expect = 4.9e-24
Identity = 62/101 (61.39%), Postives = 68/101 (67.33%), Query Frame = 2


Query: 89  TAVDFTGD----RQFLHLPPESVCCSGSGSIAECQLAAE-FEIPSEISRRILATTRRYIG 148
           +AV+ TGD      F+     S+    +GSIAEC    E  E  S+ISRRILA  ++YI 
Sbjct: 19  SAVESTGDFGDSLDFVRAGSSSLFSGCTGSIAECIAEEEEMEFDSDISRRILAQ-KKYIS 78

Query: 149 YSAMRRNSVPCSRRGASYYNCQAGAPANPYSRGCSAITRCR 208
           Y AMRRNSVPCSRRGASYYNCQ GA ANPYSRGCS ITRCR
Sbjct: 79  YGAMRRNSVPCSRRGASYYNCQRGAQANPYSRGCSTITRCR 118

Query: 209  268
           
Sbjct: 139  118

Query: 269  328
           
Sbjct: 199  118

Query: 329  377
           
Sbjct: 259  118

[Close]

BLAST of WMU43923 vs. Swiss-Prot
Match: RLF23_ARATH (Rapid alkalinization factor 23 OS=Arabidopsis thaliana GN=RALF23 PE=1 SV=1)

HSP 1 Score: 112.1 bits (279), Expect = 4.9e-24
Identity = 62/112 (55.36%), Postives = 71/112 (63.39%), Query Frame = 2


Query: 92  AVDFTGDRQFLHLPPESVCCSGSGSIAECQLAA-----------------EFEIPSEISR 151
           + +F GD      PP    C G+  IAEC ++A                 EFE+ SEI+R
Sbjct: 33  STEFAGD-----FPPFETECRGT--IAECSVSAALGDGGDLFYGGGEMGEEFEMDSEINR 92

Query: 152 RILATTRRYIGYSAMRRNSVPCSRRGASYYNCQAGAPANPYSRGCSAITRCR 211
           RILAT RRYI Y A+RRN++PCSRRGASYYNC+ GA ANPYSRGCSAITRCR
Sbjct: 93  RILAT-RRYISYGALRRNTIPCSRRGASYYNCRRGAQANPYSRGCSAITRCR 136

Query: 212  271
           
Sbjct: 153  136

Query: 272  331
           
Sbjct: 213  136

Query: 332  377
           
Sbjct: 273  136

[Close]

BLAST of WMU43923 vs. Swiss-Prot
Match: RLF1_ARATH (Protein RALF-like 1 OS=Arabidopsis thaliana GN=RALF1 PE=1 SV=1)

HSP 1 Score: 109.4 bits (272), Expect = 3.2e-23
Identity = 54/72 (75.00%), Postives = 58/72 (80.56%), Query Frame = 2


Query: 161 GSIAECQLAAEFEIPSEISRRILATTRRYIGYSAMRRNSVPCSRRGASYYNCQAGAPANP 220
           GSIAEC  A E E+ SEI+RRILATT+ YI Y +++RNSVPCSRRGASYYNCQ GA ANP
Sbjct: 49  GSIAECIGAEEEEMDSEINRRILATTK-YISYQSLKRNSVPCSRRGASYYNCQNGAQANP 108

Query: 221 YSRGCSAITRCR 280
           YSRGCS I RCR
Sbjct: 109 YSRGCSKIARCR 119

Query: 281  340
           
Sbjct: 169  119

Query: 341  377
           
Sbjct: 229  119

[Close]

BLAST of WMU43923 vs. Swiss-Prot
Match: RALF_TOBAC (Rapid alkalinization factor OS=Nicotiana tabacum GN=RALF PE=1 SV=1)

HSP 1 Score: 105.1 bits (261), Expect = 6.0e-22
Identity = 52/73 (71.23%), Postives = 58/73 (79.45%), Query Frame = 2


Query: 161 GSIAECQLAAE-FEIPSEISRRILATTRRYIGYSAMRRNSVPCSRRGASYYNCQAGAPAN 220
           GSI EC    E FE+ SE +RRILAT ++YI Y A+++NSVPCSRRGASYYNC+ GA AN
Sbjct: 43  GSIGECIAEEEEFELDSESNRRILAT-KKYISYGALQKNSVPCSRRGASYYNCKPGAQAN 102

Query: 221 PYSRGCSAITRCR 280
           PYSRGCSAITRCR
Sbjct: 103 PYSRGCSAITRCR 114

Query: 281  340
           
Sbjct: 163  114

Query: 341  377
           
Sbjct: 223  114

[Close]

BLAST of WMU43923 vs. NCBI nr
Match: gi|147862659|emb|CAN83593.1| (hypothetical protein VITISV_037723 [Vitis vinifera])

HSP 1 Score: 122.9 bits (307), Expect = 4.5e-25
Identity = 64/99 (64.65%), Postives = 74/99 (74.75%), Query Frame = 2


Query: 89  TAVDFTGDR---QFLHLPPESVCCSGSGSIAECQLAAEFEIPSEISRRILATTRRYIGYS 148
           +AVDF+GD    Q   +P  S C    GSIAEC    EFE+ SEI+RRILA+ +RYI Y 
Sbjct: 23  SAVDFSGDHLQFQLGWIPARSAC---QGSIAECLAGEEFEMDSEINRRILAS-KRYISYG 82

Query: 149 AMRRNSVPCSRRGASYYNCQAGAPANPYSRGCSAITRCR 208
           A+ RNSVPCSRRGASYYNC+ GA ANPY+RGCSAITRCR
Sbjct: 83  ALSRNSVPCSRRGASYYNCRPGAQANPYTRGCSAITRCR 117

Query: 209  268
           
Sbjct: 143  117

Query: 269  328
           
Sbjct: 203  117

Query: 329  377
           
Sbjct: 263  117

[Close]

BLAST of WMU43923 vs. NCBI nr
Match: gi|923552414|ref|XP_013737067.1| (PREDICTED: protein RALF-like 33 [Brassica napus])

HSP 1 Score: 122.9 bits (307), Expect = 4.5e-25
Identity = 67/96 (69.79%), Postives = 75/96 (78.12%), Query Frame = 2


Query: 92  AVDFTGDRQFLHLPPESVCCSGSGSIAECQLAA-EFEIPSEISRRILATTRRYIGYSAMR 151
           +VDF  D     +P E+ C   +GSIAEC  AA EFE+ SEISRRILATTR YI Y A+R
Sbjct: 23  SVDFAAD----FMPFETEC---TGSIAECTAAAAEFEMDSEISRRILATTR-YISYGALR 82

Query: 152 RNSVPCSRRGASYYNCQAGAPANPYSRGCSAITRCR 211
           RN+VPCSRRGASYYNC+ GA ANPYSRGCSAITRCR
Sbjct: 83  RNTVPCSRRGASYYNCRRGAQANPYSRGCSAITRCR 110

Query: 212  271
           
Sbjct: 143  110

Query: 272  331
           
Sbjct: 203  110

Query: 332  377
           
Sbjct: 263  110

[Close]

BLAST of WMU43923 vs. NCBI nr
Match: gi|297736875|emb|CBI26076.3| (unnamed protein product [Vitis vinifera])

HSP 1 Score: 122.9 bits (307), Expect = 4.5e-25
Identity = 64/99 (64.65%), Postives = 74/99 (74.75%), Query Frame = 2


Query: 89  TAVDFTGDR---QFLHLPPESVCCSGSGSIAECQLAAEFEIPSEISRRILATTRRYIGYS 148
           +AVDF+GD    Q   +P  S C    GSIAEC    EFE+ SEI+RRILA+ +RYI Y 
Sbjct: 57  SAVDFSGDHLQFQLGWIPARSAC---QGSIAECLAGEEFEMDSEINRRILAS-KRYISYG 116

Query: 149 AMRRNSVPCSRRGASYYNCQAGAPANPYSRGCSAITRCR 208
           A+ RNSVPCSRRGASYYNC+ GA ANPY+RGCSAITRCR
Sbjct: 117 ALSRNSVPCSRRGASYYNCRPGAQANPYTRGCSAITRCR 151

Query: 209  268
           
Sbjct: 177  151

Query: 269  328
           
Sbjct: 237  151

Query: 329  377
           
Sbjct: 297  151

[Close]

BLAST of WMU43923 vs. NCBI nr
Match: gi|225432308|ref|XP_002273386.1| (PREDICTED: protein RALF-like 33 [Vitis vinifera])

HSP 1 Score: 122.9 bits (307), Expect = 4.5e-25
Identity = 64/99 (64.65%), Postives = 74/99 (74.75%), Query Frame = 2


Query: 89  TAVDFTGDR---QFLHLPPESVCCSGSGSIAECQLAAEFEIPSEISRRILATTRRYIGYS 148
           +AVDF+GD    Q   +P  S C    GSIAEC    EFE+ SEI+RRILA+ +RYI Y 
Sbjct: 23  SAVDFSGDHLQFQLGWIPARSAC---QGSIAECLAGEEFEMDSEINRRILAS-KRYISYG 82

Query: 149 AMRRNSVPCSRRGASYYNCQAGAPANPYSRGCSAITRCR 208
           A+ RNSVPCSRRGASYYNC+ GA ANPY+RGCSAITRCR
Sbjct: 83  ALSRNSVPCSRRGASYYNCRPGAQANPYTRGCSAITRCR 117

Query: 209  268
           
Sbjct: 143  117

Query: 269  328
           
Sbjct: 203  117

Query: 329  377
           
Sbjct: 263  117

[Close]

BLAST of WMU43923 vs. NCBI nr
Match: gi|685291538|ref|XP_009136714.1| (PREDICTED: protein RALF-like 33 [Brassica rapa])

HSP 1 Score: 122.1 bits (305), Expect = 7.6e-25
Identity = 66/96 (68.75%), Postives = 75/96 (78.12%), Query Frame = 2


Query: 92  AVDFTGDRQFLHLPPESVCCSGSGSIAECQLA-AEFEIPSEISRRILATTRRYIGYSAMR 151
           +VDF  D     +P E+ C   +GSIAEC  A AEFE+ SEI+RRILATTR YI Y A+R
Sbjct: 23  SVDFAAD----FMPFETEC---TGSIAECSTATAEFEMDSEINRRILATTR-YISYGALR 82

Query: 152 RNSVPCSRRGASYYNCQAGAPANPYSRGCSAITRCR 211
           RN+VPCSRRGASYYNC+ GA ANPYSRGCSAITRCR
Sbjct: 83  RNTVPCSRRGASYYNCRRGAQANPYSRGCSAITRCR 110

Query: 212  271
           
Sbjct: 143  110

Query: 272  331
           
Sbjct: 203  110

Query: 332  377
           
Sbjct: 263  110

The following BLAST results are available for this feature:

BLAST of WMU43923 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G15800.1	1.1e-26	69.77	ralf-like 33	[more]
AT3G05490.1	2.8e-25	61.39	ralf-like 22	[more]
AT3G16570.1	2.8e-25	55.36	rapid alkalinization factor 23	[more]
AT1G02900.1	1.8e-24	75.00	rapid alkalinization factor 1	[more]
AT1G28270.1	2.8e-17	53.09	ralf-like 4	[more]

BLAST of WMU43923 vs. Swiss-Prot


Match Name	E-value	Identity	Description
RLF33_ARATH	2.0e-25	69.77	Protein RALF-like 33 OS=Arabidopsis thaliana GN=RALFL33 PE=2 SV=1	[more]
RLF22_ARATH	4.9e-24	61.39	Protein RALF-like 22 OS=Arabidopsis thaliana GN=RALFL22 PE=3 SV=1	[more]
RLF23_ARATH	4.9e-24	55.36	Rapid alkalinization factor 23 OS=Arabidopsis thaliana GN=RALF23 PE=1 SV=1	[more]
RLF1_ARATH	3.2e-23	75.00	Protein RALF-like 1 OS=Arabidopsis thaliana GN=RALF1 PE=1 SV=1	[more]
RALF_TOBAC	6.0e-22	71.23	Rapid alkalinization factor OS=Nicotiana tabacum GN=RALF PE=1 SV=1	[more]

BLAST of WMU43923 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|147862659\|emb\|CAN83593.1\|	4.5e-25	64.65	hypothetical protein VITISV_037723 [Vitis vinifera]	[more]
gi\|923552414\|ref\|XP_013737067.1\|	4.5e-25	69.79	PREDICTED: protein RALF-like 33 [Brassica napus]	[more]
gi\|297736875\|emb\|CBI26076.3\|	4.5e-25	64.65	unnamed protein product [Vitis vinifera]	[more]
gi\|225432308\|ref\|XP_002273386.1\|	4.5e-25	64.65	PREDICTED: protein RALF-like 33 [Vitis vinifera]	[more]
gi\|685291538\|ref\|XP_009136714.1\|	7.6e-25	68.75	PREDICTED: protein RALF-like 33 [Brassica rapa]	[more]

Relationships

This transcribed_cluster is associated with the following gene feature(s):


Feature Name	Unique Name	Type
Cla021738	Cla021738	gene

The following EST feature(s) are a part of this transcribed_cluster:


Feature Name	Unique Name	Type
S3_0092309	S3_0092309	EST
S3_0146376	S3_0146376	EST