WMU16404 (transcribed_cluster) Watermelon (97103) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	WMU16404
Type	transcribed_cluster
Organism	Citrullus. lanatus (Watermelon (97103) v1)
Description	Gamma-tubulin complex component
Location	Chr5 : 11859901 .. 11868527 (+)
Sequence length	725

Genome Viewer

Sequences

The following sequences are available for this feature:

transcribed_cluster sequence

CATATGCCATATCTAACTGATGCACTACACACATGTTTCTTATCTGAAGAAACCCGATCTGTGGCTGGTATCATTAATCAAATCTTGCAATGTGCTCTAGACCTCCGATGTTGTTTTACCGGTGACATGTGGAATACCCAAGTTGATAATGCAGCTTCTTCGAGGAGACTTTCTGGGATCAACAAGTCTCAGGTACTTGTCATAAAGAAGAGATTTGACACAAACATGAAGGAATTGCACTTATTTTATTTGAAGTCACCTAAGGTTGGAGAGTACGGGCTATCTCGACTTTGGGAATGTCTCAATTACAATTATCACTACTCTGACACTGGTAATGAAATGAGTTACTATGCTTTCTCAGTCTAATGGTAATTCAAATCATAAAAGGTGGTGGGGGGATCTTTATAATGTTTCTGAAATTGAGTTAGATAGGTTCGAGGCGGGGTAGTTTCTATGATGGTTGAATGTTTGTTATTAGAACCATGTAGCATTCAAGATGTCTCTAGTGATGATGCTATGTTGGCCCTACACACAAAGGATTGGTTCTTGTAATGAGTGTAGTAATATTATTACTTTGGTCGTTCTTTTTTTATGAGTGAAGTGTGCATAAGATGCATGGGTGGCTTTCCCTTAGTTTGTGTTATAGAAAATTAGTCTCTCTTGTCATCTTGTTGCTGTCTTTGTTGGAAAAGACTGGTATTGCACTGTTTAATATAGTGTGTATT

Library


Library category	ESTs
Watermelon normalization and subtraction library	1
watermelon fruit 10d library	3

Homology

[Close]

BLAST of WMU16404 vs. TAIR10
Match: AT3G43610.1 (AT3G43610.1 Spc97 / Spc98 family of spindle pole body (SBP) component)

HSP 1 Score: 116.7 bits (291), Expect = 2.0e-26
Identity = 57/119 (47.90%), Postives = 83/119 (69.75%), Query Frame = 1


Query: 1    HMPYLTDALHTCFLSEETRSVAGIINQILQCALDLRCCFTGDMWNT-QVDNAASSRRLSG 60
            HM YL++AL  CFLS+ET+ ++ II  ILQCALD R C    + +T +V N + ++ L G
Sbjct: 1087 HMAYLSEALRICFLSDETQIISNIIENILQCALDFRSCLPRGIQSTDRVPNDSWTKTL-G 1146

Query: 61   INKSQVLVIKKRFDTNMKELHLFYLKSPKVGEYGLSRLWECLNYNYHYSDTGNEMSYYA 120
            IN SQV+++K+ FD  +KELH  +L+SPK G+YGLSR W+ LN+N +YSD  ++ + ++
Sbjct: 1147 INTSQVMMVKQNFDKELKELHKCHLRSPKHGKYGLSRFWDYLNFNLYYSDILHDSNIFS 1204

Query: 121   180
            
Sbjct: 1207  1204

Query: 181   240
            
Sbjct: 1267  1204

Query: 241   300
            
Sbjct: 1327  1204

Query: 301   355
            
Sbjct: 1387  1204

[Close]

BLAST of WMU16404 vs. TrEMBL
Match: M5WK50_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa000377mg PE=4 SV=1)

HSP 1 Score: 162.9 bits (411), Expect = 4.8e-37
Identity = 78/121 (64.46%), Postives = 90/121 (74.38%), Query Frame = 1


Query: 1    HMPYLTDALHTCFLSEETRSVAGIINQILQCALDLRCCFTGDMWNTQVDNAASSRRLSGI 60
            H+ YL D+L  CFLS+ETR +A II  ILQCALD R C TG+MW+          RLSGI
Sbjct: 1107 HLAYLIDSLDICFLSDETRPIARIIESILQCALDFRSCLTGEMWDVGTSQGNLIARLSGI 1166

Query: 61   NKSQVLVIKKRFDTNMKELHLFYLKSPKVGEYGLSRLWECLNYNYHYSDTGNEMSYYAFS 120
            N SQV+VIK+ FD NMKELHL YLKSPK G++GLS  WE LNYN +YSD GNEM+YYAFS
Sbjct: 1167 NISQVVVIKQMFDKNMKELHLCYLKSPKHGKFGLSHFWEYLNYNKYYSDVGNEMAYYAFS 1226

Query: 121  V 180
            V
Sbjct: 1227 V 1227

Query: 181   240
            
Sbjct: 1287  1227

Query: 241   300
            
Sbjct: 1347  1227

Query: 301   360
            
Sbjct: 1407  1227

Query: 361   364
            
Sbjct: 1467  1227

[Close]

BLAST of WMU16404 vs. TrEMBL
Match: U5G4E8_POPTR (Gamma-tubulin complex component OS=Populus trichocarpa GN=POPTR_0009s15540g PE=3 SV=1)

HSP 1 Score: 154.5 bits (389), Expect = 1.7e-34
Identity = 75/118 (63.56%), Postives = 86/118 (72.88%), Query Frame = 1


Query: 1    HMPYLTDALHTCFLSEETRSVAGIINQILQCALDLRCCFTGDMWNTQVDNAASSRRLSGI 60
            HM YLTD+LH CFLS ETRSVA II  ILQCA + R CFTG MW+  +D      +LS I
Sbjct: 926  HMAYLTDSLHICFLSNETRSVAIIIESILQCAFEFRSCFTGGMWDMGLDQGDLLGKLSRI 985

Query: 61   NKSQVLVIKKRFDTNMKELHLFYLKSPKVGEYGLSRLWECLNYNYHYSDTGNEMSYYA 120
            N SQVL IK++FD N+K+LHL YLK PK GE+GLSR W  LNYN +YSD GNEM  YA
Sbjct: 986  NISQVLAIKQKFDKNLKQLHLCYLKFPKHGEFGLSRFWGYLNYNKYYSDVGNEMDLYA 1043

Query: 121   180
            
Sbjct: 1046  1043

Query: 181   240
            
Sbjct: 1106  1043

Query: 241   300
            
Sbjct: 1166  1043

Query: 301   355
            
Sbjct: 1226  1043

[Close]

BLAST of WMU16404 vs. TrEMBL
Match: U5G6S0_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0009s15540g PE=4 SV=1)

HSP 1 Score: 154.5 bits (389), Expect = 1.7e-34
Identity = 75/118 (63.56%), Postives = 86/118 (72.88%), Query Frame = 1


Query: 1    HMPYLTDALHTCFLSEETRSVAGIINQILQCALDLRCCFTGDMWNTQVDNAASSRRLSGI 60
            HM YLTD+LH CFLS ETRSVA II  ILQCA + R CFTG MW+  +D      +LS I
Sbjct: 1077 HMAYLTDSLHICFLSNETRSVAIIIESILQCAFEFRSCFTGGMWDMGLDQGDLLGKLSRI 1136

Query: 61   NKSQVLVIKKRFDTNMKELHLFYLKSPKVGEYGLSRLWECLNYNYHYSDTGNEMSYYA 120
            N SQVL IK++FD N+K+LHL YLK PK GE+GLSR W  LNYN +YSD GNEM  YA
Sbjct: 1137 NISQVLAIKQKFDKNLKQLHLCYLKFPKHGEFGLSRFWGYLNYNKYYSDVGNEMDLYA 1194

Query: 121   180
            
Sbjct: 1197  1194

Query: 181   240
            
Sbjct: 1257  1194

Query: 241   300
            
Sbjct: 1317  1194

Query: 301   355
            
Sbjct: 1377  1194

[Close]

BLAST of WMU16404 vs. TrEMBL
Match: A0A0D2QBQ1_GOSRA (Gamma-tubulin complex component OS=Gossypium raimondii GN=B456_009G080700 PE=3 SV=1)

HSP 1 Score: 150.2 bits (378), Expect = 3.2e-33
Identity = 74/121 (61.16%), Postives = 87/121 (71.90%), Query Frame = 1


Query: 1   HMPYLTDALHTCFLSEETRSVAGIINQILQCALDLRCCFTGDMWNTQVDNAASSRRLSGI 60
           HM YLTD+LH CFLS+ET+ +A  I  ILQCALD R C TGD+WN  +       +LS I
Sbjct: 849 HMSYLTDSLHICFLSDETKPIASTIENILQCALDFRSCLTGDIWNVGLAEGDLQDKLSKI 908

Query: 61  NKSQVLVIKKRFDTNMKELHLFYLKSPKVGEYGLSRLWECLNYNYHYSDTGNEMSYYAFS 120
           N SQVL IK++FD N+KELHL YLKSPK GE GLS  W  LNYN +Y +  NEMSYYAFS
Sbjct: 909 NISQVLFIKEKFDKNLKELHLLYLKSPKHGESGLSYFWGYLNYNDYYFNE-NEMSYYAFS 968

Query: 121 V 180
           +
Sbjct: 969 I 968

Query: 181  240
           
Sbjct: 1029  968

Query: 241  300
           
Sbjct: 1089  968

Query: 301  360
           
Sbjct: 1149  968

Query: 361  364
           
Sbjct: 1209  968

[Close]

BLAST of WMU16404 vs. TrEMBL
Match: A0A0D2TLP4_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_009G080700 PE=4 SV=1)

HSP 1 Score: 150.2 bits (378), Expect = 3.2e-33
Identity = 74/121 (61.16%), Postives = 87/121 (71.90%), Query Frame = 1


Query: 1    HMPYLTDALHTCFLSEETRSVAGIINQILQCALDLRCCFTGDMWNTQVDNAASSRRLSGI 60
            HM YLTD+LH CFLS+ET+ +A  I  ILQCALD R C TGD+WN  +       +LS I
Sbjct: 1121 HMSYLTDSLHICFLSDETKPIASTIENILQCALDFRSCLTGDIWNVGLAEGDLQDKLSKI 1180

Query: 61   NKSQVLVIKKRFDTNMKELHLFYLKSPKVGEYGLSRLWECLNYNYHYSDTGNEMSYYAFS 120
            N SQVL IK++FD N+KELHL YLKSPK GE GLS  W  LNYN +Y +  NEMSYYAFS
Sbjct: 1181 NISQVLFIKEKFDKNLKELHLLYLKSPKHGESGLSYFWGYLNYNDYYFNE-NEMSYYAFS 1240

Query: 121  V 180
            +
Sbjct: 1241 I 1240

Query: 181   240
            
Sbjct: 1301  1240

Query: 241   300
            
Sbjct: 1361  1240

Query: 301   360
            
Sbjct: 1421  1240

Query: 361   364
            
Sbjct: 1481  1240

[Close]

BLAST of WMU16404 vs. NCBI nr
Match: gi|659114495|ref|XP_008457080.1| (PREDICTED: uncharacterized protein LOC103496848 [Cucumis melo])

HSP 1 Score: 225.3 bits (573), Expect = 1.1e-55
Identity = 106/121 (87.60%), Postives = 113/121 (93.39%), Query Frame = 1


Query: 1    HMPYLTDALHTCFLSEETRSVAGIINQILQCALDLRCCFTGDMWNTQVDNAASSRRLSGI 60
            HM YLTDALHTCFLSEET+ VA IINQILQCALDLRCCFT DMWNTQVD+AASSRRLS I
Sbjct: 1102 HMAYLTDALHTCFLSEETQFVASIINQILQCALDLRCCFTSDMWNTQVDHAASSRRLSEI 1161

Query: 61   NKSQVLVIKKRFDTNMKELHLFYLKSPKVGEYGLSRLWECLNYNYHYSDTGNEMSYYAFS 120
            NK+QVLVIKKRFD NMKELHL +LKSPKVG++GLSRLWECLNYNYHYS+TGNEMSYYA S
Sbjct: 1162 NKAQVLVIKKRFDRNMKELHLCHLKSPKVGDFGLSRLWECLNYNYHYSNTGNEMSYYALS 1221

Query: 121  V 180
            V
Sbjct: 1222 V 1222

Query: 181   240
            
Sbjct: 1282  1222

Query: 241   300
            
Sbjct: 1342  1222

Query: 301   360
            
Sbjct: 1402  1222

Query: 361   364
            
Sbjct: 1462  1222

[Close]

BLAST of WMU16404 vs. NCBI nr
Match: gi|778679754|ref|XP_011651193.1| (PREDICTED: uncharacterized protein LOC101209612 [Cucumis sativus])

HSP 1 Score: 222.6 bits (566), Expect = 7.3e-55
Identity = 104/121 (85.95%), Postives = 113/121 (93.39%), Query Frame = 1


Query: 1    HMPYLTDALHTCFLSEETRSVAGIINQILQCALDLRCCFTGDMWNTQVDNAASSRRLSGI 60
            HM YLTDALHTCFLSEET+SVA  INQ+LQCALDLRCCFTGDMWNTQVD+AASSRRLS I
Sbjct: 1103 HMAYLTDALHTCFLSEETQSVASTINQLLQCALDLRCCFTGDMWNTQVDHAASSRRLSEI 1162

Query: 61   NKSQVLVIKKRFDTNMKELHLFYLKSPKVGEYGLSRLWECLNYNYHYSDTGNEMSYYAFS 120
            NK+QVLVIKKRFD ++KELHL +LKSPKVG++GLSRLWECLNYNYHY  TGNEMSYYAFS
Sbjct: 1163 NKAQVLVIKKRFDRSIKELHLCHLKSPKVGDFGLSRLWECLNYNYHYPITGNEMSYYAFS 1222

Query: 121  V 180
            V
Sbjct: 1223 V 1223

Query: 181   240
            
Sbjct: 1283  1223

Query: 241   300
            
Sbjct: 1343  1223

Query: 301   360
            
Sbjct: 1403  1223

Query: 361   364
            
Sbjct: 1463  1223

[Close]

BLAST of WMU16404 vs. NCBI nr
Match: gi|645218491|ref|XP_008230919.1| (PREDICTED: uncharacterized protein LOC103330137 [Prunus mume])

HSP 1 Score: 164.9 bits (416), Expect = 1.8e-37
Identity = 78/121 (64.46%), Postives = 90/121 (74.38%), Query Frame = 1


Query: 1    HMPYLTDALHTCFLSEETRSVAGIINQILQCALDLRCCFTGDMWNTQVDNAASSRRLSGI 60
            H+ YL D+L  CFLS+ETR +A II  ILQCALD R C TG+MW+          RLSGI
Sbjct: 1107 HLAYLIDSLDICFLSDETRPIARIIESILQCALDFRSCLTGEMWDAGTSQGNLMARLSGI 1166

Query: 61   NKSQVLVIKKRFDTNMKELHLFYLKSPKVGEYGLSRLWECLNYNYHYSDTGNEMSYYAFS 120
            N SQV+VIK+ FD NMKELHL YLKSPK G++GLS  WE LNYN +YSD GNEM+YYAFS
Sbjct: 1167 NISQVVVIKQTFDKNMKELHLCYLKSPKHGKFGLSHFWEYLNYNKYYSDVGNEMAYYAFS 1226

Query: 121  V 180
            V
Sbjct: 1227 V 1227

Query: 181   240
            
Sbjct: 1287  1227

Query: 241   300
            
Sbjct: 1347  1227

Query: 301   360
            
Sbjct: 1407  1227

Query: 361   364
            
Sbjct: 1467  1227

[Close]

BLAST of WMU16404 vs. NCBI nr
Match: gi|595842438|ref|XP_007208419.1| (hypothetical protein PRUPE_ppa000377mg [Prunus persica])

HSP 1 Score: 164.5 bits (415), Expect = 2.3e-37
Identity = 78/121 (64.46%), Postives = 90/121 (74.38%), Query Frame = 1


Query: 1    HMPYLTDALHTCFLSEETRSVAGIINQILQCALDLRCCFTGDMWNTQVDNAASSRRLSGI 60
            H+ YL D+L  CFLS+ETR +A II  ILQCALD R C TG+MW+          RLSGI
Sbjct: 1107 HLAYLIDSLDICFLSDETRPIARIIESILQCALDFRSCLTGEMWDVGTSQGNLIARLSGI 1166

Query: 61   NKSQVLVIKKRFDTNMKELHLFYLKSPKVGEYGLSRLWECLNYNYHYSDTGNEMSYYAFS 120
            N SQV+VIK+ FD NMKELHL YLKSPK G++GLS  WE LNYN +YSD GNEM+YYAFS
Sbjct: 1167 NISQVVVIKQMFDKNMKELHLCYLKSPKHGKFGLSHFWEYLNYNKYYSDVGNEMAYYAFS 1226

Query: 121  V 180
            V
Sbjct: 1227 V 1227

Query: 181   240
            
Sbjct: 1287  1227

Query: 241   300
            
Sbjct: 1347  1227

Query: 301   360
            
Sbjct: 1407  1227

Query: 361   364
            
Sbjct: 1467  1227

[Close]

BLAST of WMU16404 vs. NCBI nr
Match: gi|658028830|ref|XP_008349856.1| (PREDICTED: uncharacterized protein LOC103413144, partial [Malus domestica])

HSP 1 Score: 163.7 bits (413), Expect = 4.0e-37
Identity = 76/121 (62.81%), Postives = 90/121 (74.38%), Query Frame = 1


Query: 1    HMPYLTDALHTCFLSEETRSVAGIINQILQCALDLRCCFTGDMWNTQVDNAASSRRLSGI 60
            HM YLTD+L  CFLS+ETR +A II  ILQCALD R C TG MW+        + RLSG+
Sbjct: 1051 HMAYLTDSLDICFLSDETRPIARIIESILQCALDFRSCLTGGMWDVGTGEGNRTARLSGM 1110

Query: 61   NKSQVLVIKKRFDTNMKELHLFYLKSPKVGEYGLSRLWECLNYNYHYSDTGNEMSYYAFS 120
            N SQV+ IK+ FD NMKELHL YLKSPK GE+GLS  WE LNYN +YSD GNEM++YAF+
Sbjct: 1111 NISQVVAIKQTFDKNMKELHLCYLKSPKHGEFGLSHFWEYLNYNKYYSDVGNEMAFYAFA 1170

Query: 121  V 180
            +
Sbjct: 1171 I 1171

Query: 181   240
            
Sbjct: 1231  1171

Query: 241   300
            
Sbjct: 1291  1171

Query: 301   360
            
Sbjct: 1351  1171

Query: 361   364
            
Sbjct: 1411  1171

The following BLAST results are available for this feature:

BLAST of WMU16404 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G43610.1	2.0e-26	47.90	Spc97 / Spc98 family of spindle pole body (SBP) component	[more]

BLAST of WMU16404 vs. TrEMBL


Match Name	E-value	Identity	Description
M5WK50_PRUPE	4.8e-37	64.46	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa000377mg PE=4 SV=1	[more]
U5G4E8_POPTR	1.7e-34	63.56	Gamma-tubulin complex component OS=Populus trichocarpa GN=POPTR_0009s15540g PE=3...	[more]
U5G6S0_POPTR	1.7e-34	63.56	Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0009s15540g PE=4 SV=1	[more]
A0A0D2QBQ1_GOSRA	3.2e-33	61.16	Gamma-tubulin complex component OS=Gossypium raimondii GN=B456_009G080700 PE=3 S...	[more]
A0A0D2TLP4_GOSRA	3.2e-33	61.16	Uncharacterized protein OS=Gossypium raimondii GN=B456_009G080700 PE=4 SV=1	[more]

BLAST of WMU16404 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659114495\|ref\|XP_008457080.1\|	1.1e-55	87.60	PREDICTED: uncharacterized protein LOC103496848 [Cucumis melo]	[more]
gi\|778679754\|ref\|XP_011651193.1\|	7.3e-55	85.95	PREDICTED: uncharacterized protein LOC101209612 [Cucumis sativus]	[more]
gi\|645218491\|ref\|XP_008230919.1\|	1.8e-37	64.46	PREDICTED: uncharacterized protein LOC103330137 [Prunus mume]	[more]
gi\|595842438\|ref\|XP_007208419.1\|	2.3e-37	64.46	hypothetical protein PRUPE_ppa000377mg [Prunus persica]	[more]
gi\|658028830\|ref\|XP_008349856.1\|	4.0e-37	62.81	PREDICTED: uncharacterized protein LOC103413144, partial [Malus domestica]	[more]

Annotated Terms

The following terms have been associated with this transcribed_cluster:

Vocabulary: **INTERPRO**
Term	Definition
IPR007259	GCP

Vocabulary: **Biological Process**
Term	Definition
GO:0000226	microtubule cytoskeleton organization
GO:0007020	microtubule nucleation

Vocabulary: **Cellular Component**
Term	Definition
GO:0000922	spindle pole
GO:0005815	microtubule organizing center

Vocabulary: **Molecular Function**
Term	Definition
GO:0043015	gamma-tubulin binding

GO Annotation

GO Assignments

This transcribed_cluster is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0000226	microtubule cytoskeleton organization
biological_process	GO:0007020	microtubule nucleation
cellular_component	GO:0005815	microtubule organizing center
cellular_component	GO:0000922	spindle pole
molecular_function	GO:0043015	gamma-tubulin binding

Relationships

This transcribed_cluster is associated with the following gene feature(s):


Feature Name	Unique Name	Type
Cla013164	Cla013164	gene

The following EST feature(s) are a part of this transcribed_cluster:


Feature Name	Unique Name	Type
AL0105M10	AL0105M10	EST
S1_0046564	S1_0046564	EST
S1_0075909	S1_0075909	EST
S1_0150371	S1_0150371	EST

InterPro

Analysis Name: InterPro Annotations of watermelon unigene v2
Date Performed: 2016-11-16


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR007259	Gamma-tubulin complex component protein	PANTHER	PTHR19302	GAMMA TUBULIN COMPLEX PROTEIN	coord: 1..118 score: 2.9
None	No IPR available	PANTHER	PTHR19302:SF37	GAMMA-TUBULIN COMPLEX COMPONENT 6	coord: 1..118 score: 2.9