MU62892 (transcribed_cluster) Melon (DHL92) v3.5.1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	MU62892
Type	transcribed_cluster
Organism	Cucumis melo (Melon (DHL92) v3.5.1)
Description	DNA-directed RNA polymerase subunit beta
Location	chr3 : 24180416 .. 24189403 (-)
Sequence length	822

Genome Viewer

Sequences

The following sequences are available for this feature:

transcribed_cluster sequence

GTTCTGCTACCTGGTCAGCTAATTACTCTTTATCTCAAGGAGAAACTGGAAGACTGGTTGCAAAAGTCGAAAAAGCTTCTTGAAGATGAAATTAGTAATCCCAGCAAAAATTTTGAATTCTGCAGCTTAGCTCATGTGAAGAAGGTCATGGACAAGAATCCATCAAAACAGATTAGTTCAGCTGTTGAAAATATGTTGAAAACTGGAAGACTCGTTACACAAACTGGCCTAGATCTGCAGCAGGCTTTACAGTTCAGGCAGAGCGACTTAATTTCTTGCGCTTTGTGTCTCATTTTCGTGCTGTTCATCGTGGAGCTTCGTTTGCTGGACTTCGCACTACAACTGTGCGGAAATTGCTACCTGAGTCTTGGGGTTTTCTTTGTCCAGTACACACTCCTGATGGTGAACCATGCGGCTTGCTGAATCATTTAACCAGCACCTGCAGAATTACGTCATACTTCAATTCTCAAGGAAAAGTTCGAGACTTTTACAAAATAAGGACGTCTATTCTAAGTGTTCTAATTGCAGTTGGAATGATACCGTCACTACCGAAACTTGTTCAAATGGGTCCTCCTGATGTTCTTAATGTGCTTTTAGATGGTCGACTCGTTGGTTATATATCATCCAATGAAGTAGAGAAAGTTGTTGCTCATTTACGTCGATTAAAAGTTTTATCTCCCCACGTGGTTTGTAGTTGTGCATTTTTCTGCTATTGCATGTTGTTCTTTTCTATTTCTCTCATTATTTTTGGGTATGTTTCAGATTCCCGACGATCTGGAAGTTGGATATGTACCTTTGAGTATAAGTGGAGCATATCCTGGT

Library


Library category	ESTs
melon CM-PEa library	1

Homology

[Close]

BLAST of MU62892 vs. Swiss-Prot
Match: NRPA2_ARATH (DNA-directed RNA polymerase I subunit 2 OS=Arabidopsis thaliana GN=NRPA2 PE=2 SV=1)

HSP 1 Score: 232.3 bits (591), Expect = 6.5e-60
Identity = 111/150 (74.00%), Postives = 129/150 (86.00%), Query Frame = 3


Query: 240 AGFTVQAERLNFLRFVSHFRAVHRGASFAGLRTTTVRKLLPESWGFLCPVHTPDGEPCGL 299
           AG+TVQAERLNFLRF+S FRAVHRGASFAGLRTTTVRKLLPESWGFLCPVHTPDG PCGL
Sbjct: 453 AGYTVQAERLNFLRFLSFFRAVHRGASFAGLRTTTVRKLLPESWGFLCPVHTPDGTPCGL 512

Query: 300 LNHLTSTCRITSYFNSQGKVRDFYKIRTSILSVLIAVGMIPSLPKLVQMGPPDVLNVLLD 359
           LNH+T T RITS F+S+G +RDF KIR S++ VL   GM+PSLPKLV+ GPP V++VLLD
Sbjct: 513 LNHMTRTSRITSQFDSKGNIRDFLKIRKSVVDVLTGAGMVPSLPKLVRAGPPKVIHVLLD 572

Query: 360 GRLVGYISSNEVEKVVAHLRRLKVLSPHVV 419
           G++VG +SSN V KVV+++RRLKV +P V+
Sbjct: 573 GQVVGTLSSNLVTKVVSYIRRLKVEAPSVI 602

Query: 420  479
           
Sbjct: 633  602

Query: 480  539
           
Sbjct: 693  602

Query: 540  599
           
Sbjct: 753  602

Query: 600  659
           
Sbjct: 813  602

Query: 660  690
           
Sbjct: 873  602

HSP 2 Score: 103.2 bits (256), Expect = 4.6e-21
Identity = 47/81 (58.02%), Postives = 63/81 (77.78%), Query Frame = 1


Query: 1   VLLPGQLITLYLKEKLEDWLQKSKKLLEDEISNPSKNFEFCSLAHVKKVMDKNPSKQISS 60
           +L+PG +IT+YLKEKLE+WL+K K LL+DE+ N +  F F SLA VKK+++KNP + I +
Sbjct: 371 ILVPGHVITIYLKEKLEEWLRKCKSLLKDELDNTNSKFSFESLADVKKLINKNPPRSIGT 430

Query: 61  AVENMLKTGRLVTQTGLDLQQ 120
           ++E +LKTG L TQ+GLDLQQ
Sbjct: 431 SIETLLKTGALKTQSGLDLQQ 451

Query: 121  180
           
Sbjct: 491  451

Query: 181  240
           
Sbjct: 551  451

Query: 241  244
           
Sbjct: 611  451

HSP 3 Score: 38.1 bits (87), Expect = 1.8e-01
Identity = 15/20 (75.00%), Postives = 16/20 (80.00%), Query Frame = 1


Query: 763 IPDDLEVGYVPLSISGAYPG 822
           IP+DLEVGYVP S+ G+YPG
Sbjct: 602 IPEDLEVGYVPTSMGGSYPG 621

[Close]

BLAST of MU62892 vs. Swiss-Prot
Match: RPA2_DICDI (DNA-directed RNA polymerase I subunit rpa2 OS=Dictyostelium discoideum GN=polr1b PE=3 SV=1)

HSP 1 Score: 140.6 bits (353), Expect = 2.6e-32
Identity = 72/146 (49.32%), Postives = 95/146 (65.07%), Query Frame = 3


Query: 231 RSAAGFTVQAERLNFLRFVSHFRAVHRGASFAGLRTTTVRKLLPESWGFLCPVHTPDGEP 290
           +  +GF V A++LNFLRF+SHFR++HRGA FA ++TT +RKL+PESWGF CPVHTPDG P
Sbjct: 434 QQTSGFCVIADKLNFLRFISHFRSIHRGAFFATMKTTAIRKLMPESWGFFCPVHTPDGAP 493

Query: 291 CGLLNHLTSTCRITSYFNSQGKVRDFYKIRTSILSVLIAVGMIPSLPKLVQMGPPDVLNV 350
           CGLLNHL+S C +T        V+   K +T + S L A GM+P    +V +     L +
Sbjct: 494 CGLLNHLSSNCLVTVDAAQDPTVQ---KAQTLLPSFLAAHGMLPI--DMVSVYQHQYLTI 553

Query: 351 LLDGRLVGYISSNEVEKVVAHLRRLK 410
            LDGR++G I     E++V  LR L+
Sbjct: 554 TLDGRVLGKIEPKAGEEMVKMLRYLR 574

Query: 411  470
           
Sbjct: 614  574

Query: 471  530
           
Sbjct: 674  574

Query: 531  590
           
Sbjct: 734  574

Query: 591  650
           
Sbjct: 794  574

Query: 651  669
           
Sbjct: 854  574

[Close]

BLAST of MU62892 vs. Swiss-Prot
Match: RPA2_MOUSE (DNA-directed RNA polymerase I subunit RPA2 OS=Mus musculus GN=Polr1b PE=1 SV=2)

HSP 1 Score: 140.2 bits (352), Expect = 3.4e-32
Identity = 75/149 (50.34%), Postives = 93/149 (62.42%), Query Frame = 3


Query: 240 AGFTVQAERLNFLRFVSHFRAVHRGASFAGLRTTTVRKLLPESWGFLCPVHTPDGEPCGL 299
           +G  V A++LNFLR++SHFR VHRGA+FA +RTTTVR+LLPESWGFLCPVHTPDG PCGL
Sbjct: 451 SGLCVVADKLNFLRYLSHFRCVHRGAAFAKMRTTTVRRLLPESWGFLCPVHTPDGAPCGL 510

Query: 300 LNHLTSTCRITSYFNSQGKVRDFYKIRTSILSVLIAVGMIPSLPKLVQMGP----PDVLN 359
           LNHLT+ C + + F              SI ++L  +G+ P     V   P     D   
Sbjct: 511 LNHLTAVCEVVTKF----------VYTASIPALLCGLGVTP-----VDTAPCRPYSDCYP 570

Query: 360 VLLDGRLVGYISSNEVEKVVAHLRRLKVL 419
           VLLDG +VG++  +   +V   LRR KVL
Sbjct: 571 VLLDGVMVGWVDKDLAPEVADTLRRFKVL 584

Query: 420  479
           
Sbjct: 631  584

Query: 480  539
           
Sbjct: 691  584

Query: 540  599
           
Sbjct: 751  584

Query: 600  659
           
Sbjct: 811  584

Query: 660  675
           
Sbjct: 871  584

HSP 2 Score: 42.0 bits (97), Expect = 1.3e-02
Identity = 29/83 (34.94%), Postives = 45/83 (54.22%), Query Frame = 1


Query: 1   VLLPGQLITLYLKEKLEDWLQKSKKLLED--EISNPSKNFEFCSLAHVKKVMDKNPSKQI 60
           VL PGQL  ++LKEK+E+WL   K +L+   + +N S N E     ++ K+   +   ++
Sbjct: 374 VLSPGQLFLMFLKEKMENWLVSIKIVLDKRAQKANVSINNE-----NLMKIF--SMGTEL 433

Query: 61  SSAVENMLKTGRLVTQTGLDLQQ 120
           +   E +L TG L ++TGL   Q
Sbjct: 434 TRPFEYLLATGNLRSKTGLGFLQ 449

Query: 121  180
           
Sbjct: 494  449

Query: 181  240
           
Sbjct: 554  449

Query: 241  244
           
Sbjct: 614  449

[Close]

BLAST of MU62892 vs. Swiss-Prot
Match: RPA2_RAT (DNA-directed RNA polymerase I subunit RPA2 OS=Rattus norvegicus GN=Polr1b PE=1 SV=1)

HSP 1 Score: 139.8 bits (351), Expect = 4.4e-32
Identity = 75/149 (50.34%), Postives = 91/149 (61.07%), Query Frame = 3


Query: 240 AGFTVQAERLNFLRFVSHFRAVHRGASFAGLRTTTVRKLLPESWGFLCPVHTPDGEPCGL 299
           +G  V A++LNF+R++SHFR VHRGA FA +RTTTVRKLLPESWGFLCPVHTPDG PCGL
Sbjct: 451 SGLCVVADKLNFIRYLSHFRCVHRGADFAKMRTTTVRKLLPESWGFLCPVHTPDGAPCGL 510

Query: 300 LNHLTSTCRITSYFNSQGKVRDFYKIRTSILSVLIAVGMIPSLPKLVQMGP----PDVLN 359
           LNHLT+ C + + F              SI ++L  +G+ P     V   P     D   
Sbjct: 511 LNHLTAVCEVVTKF----------VYTASIPALLCGLGVTP-----VDAAPCRPYSDCYP 570

Query: 360 VLLDGRLVGYISSNEVEKVVAHLRRLKVL 419
           VLLDG +VG++      +V   LRR KVL
Sbjct: 571 VLLDGVMVGWVDKELAPEVADTLRRFKVL 584

Query: 420  479
           
Sbjct: 631  584

Query: 480  539
           
Sbjct: 691  584

Query: 540  599
           
Sbjct: 751  584

Query: 600  659
           
Sbjct: 811  584

Query: 660  675
           
Sbjct: 871  584

[Close]

BLAST of MU62892 vs. Swiss-Prot
Match: RPA2_YEAST (DNA-directed RNA polymerase I subunit RPA135 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=RPA135 PE=1 SV=1)

HSP 1 Score: 136.3 bits (342), Expect = 4.9e-31
Identity = 70/147 (47.62%), Postives = 94/147 (63.95%), Query Frame = 3


Query: 231 RSAAGFTVQAERLNFLRFVSHFRAVHRGASFAGLRTTTVRKLLPESWGFLCPVHTPDGEP 290
           +  +G+TV AE++NF RF+SHFR VHRG+ FA L+TTTVRKLLPESWGFLCPVHTPDG P
Sbjct: 479 QQVSGYTVVAEKINFYRFISHFRMVHRGSFFAQLKTTTVRKLLPESWGFLCPVHTPDGSP 538

Query: 291 CGLLNHLTSTCRITSYFNSQGKVRDFYKIRTSILSVLIAVGMIPSLPKLVQMGPPDVLNV 350
           CGLLNH    CRI++    Q  V       + I S+L ++G+ P+         P +  V
Sbjct: 539 CGLLNHFAHKCRIST---QQSDV-------SRIPSILYSLGVAPASHTFA--AGPSLCCV 598

Query: 351 LLDGRLVGYISSNEVEKVVAHLRRLKV 410
            +DG+++G++S  + + +   LR  KV
Sbjct: 599 QIDGKIIGWVSHEQGKIIADTLRYWKV 613

Query: 411  470
           
Sbjct: 659  613

Query: 471  530
           
Sbjct: 719  613

Query: 531  590
           
Sbjct: 779  613

Query: 591  650
           
Sbjct: 839  613

Query: 651  672
           
Sbjct: 899  613

HSP 2 Score: 43.9 bits (102), Expect = 3.3e-03
Identity = 29/81 (35.80%), Postives = 46/81 (56.79%), Query Frame = 1


Query: 1   VLLPGQLITLYLKEKLEDWLQKSKKLLEDEISNPSKNFEFCSLAHVKKVMDKNPSKQISS 60
           VLL G L  + LKEK++++LQ     +  +I N      F    ++ +V+ +  ++ I S
Sbjct: 402 VLLGGFLYGMILKEKIDEYLQNIIAQVRMDI-NRGMAINFKDKRYMSRVLMR-VNENIGS 461

Query: 61  AVENMLKTGRLVTQTGLDLQQ 120
            ++  L TG LV+Q+GLDLQQ
Sbjct: 462 KMQYFLSTGNLVSQSGLDLQQ 480

Query: 121  180
           
Sbjct: 522  480

Query: 181  240
           
Sbjct: 582  480

Query: 241  244
           
Sbjct: 642  480

[Close]

BLAST of MU62892 vs. TrEMBL
Match: A0A0A0KIZ8_CUCSA (DNA-directed RNA polymerase subunit beta OS=Cucumis sativus GN=Csa_6G400300 PE=3 SV=1)

HSP 1 Score: 293.9 bits (751), Expect = 2.0e-76
Identity = 144/150 (96.00%), Postives = 148/150 (98.67%), Query Frame = 3


Query: 240 AGFTVQAERLNFLRFVSHFRAVHRGASFAGLRTTTVRKLLPESWGFLCPVHTPDGEPCGL 299
           AGFTVQAERLNFLRFVSHFRAVHRGASFAGLRTTTVRKLLPESWGFLCPVHTPDGEPCGL
Sbjct: 527 AGFTVQAERLNFLRFVSHFRAVHRGASFAGLRTTTVRKLLPESWGFLCPVHTPDGEPCGL 586

Query: 300 LNHLTSTCRITSYFNSQGKVRDFYKIRTSILSVLIAVGMIPSLPKLVQMGPPDVLNVLLD 359
           LNHLTSTCRITS+FNSQGKVRDFYKIRTSILSVLIAVGMIPSLPKLVQMG PD LNVLLD
Sbjct: 587 LNHLTSTCRITSHFNSQGKVRDFYKIRTSILSVLIAVGMIPSLPKLVQMGLPDFLNVLLD 646

Query: 360 GRLVGYISSNEVEKVVAHLRRLKVLSPHVV 419
           GR+VGYISSNEVEK+VAHLRRLKVLSPHV+
Sbjct: 647 GRIVGYISSNEVEKIVAHLRRLKVLSPHVI 676

Query: 420  479
           
Sbjct: 707  676

Query: 480  539
           
Sbjct: 767  676

Query: 540  599
           
Sbjct: 827  676

Query: 600  659
           
Sbjct: 887  676

Query: 660  690
           
Sbjct: 947  676

[Close]

BLAST of MU62892 vs. TrEMBL
Match: A0A0A0KIZ8_CUCSA (DNA-directed RNA polymerase subunit beta OS=Cucumis sativus GN=Csa_6G400300 PE=3 SV=1)

HSP 1 Score: 159.8 bits (403), Expect = 4.6e-36
Identity = 80/85 (94.12%), Postives = 81/85 (95.29%), Query Frame = 1


Query: 1   VLLPGQLITLYLKEKLEDWLQKSKKLLEDEISNPSKNFEFCSLAHVKKVMDKNPSKQISS 60
           VLLPGQLITLYLKEKLEDWLQK KKLLEDEISNPSKNFEFCSLAHVKKVMDKNPSKQ+SS
Sbjct: 445 VLLPGQLITLYLKEKLEDWLQKVKKLLEDEISNPSKNFEFCSLAHVKKVMDKNPSKQVSS 504

Query: 61  AVENMLKTGRLVTQTGLDLQQALQF 120
           AVENMLKTGRLVTQTGLDLQQ   F
Sbjct: 505 AVENMLKTGRLVTQTGLDLQQRAGF 529

Query: 121  180
           
Sbjct: 565  529

Query: 181  240
           
Sbjct: 625  529

Query: 241  256
           
Sbjct: 685  529

HSP 2 Score: 45.4 bits (106), Expect = 1.3e-01
Identity = 20/20 (100.00%), Postives = 20/20 (100.00%), Query Frame = 1


Query: 763 IPDDLEVGYVPLSISGAYPG 822
           IPDDLEVGYVPLSISGAYPG
Sbjct: 676 IPDDLEVGYVPLSISGAYPG 695

HSP 3 Score: 252.7 bits (644), Expect = 5.2e-64
Identity = 122/144 (84.72%), Postives = 134/144 (93.06%), Query Frame = 3


Query: 240 AGFTVQAERLNFLRFVSHFRAVHRGASFAGLRTTTVRKLLPESWGFLCPVHTPDGEPCGL 299
           AGFTVQAERLN+LRF+SHFRAVHRG SFAGLRTT+VRKLLPESWGFLCPVHTPDGEPCGL
Sbjct: 449 AGFTVQAERLNYLRFLSHFRAVHRGTSFAGLRTTSVRKLLPESWGFLCPVHTPDGEPCGL 508

Query: 300 LNHLTSTCRITSYFNSQGKVRDFYKIRTSILSVLIAVGMIPSLPKLVQMGPPDVLNVLLD 359
           LNHLT TCRITSYF+SQG  +DF+KIR SILSVL+ VGM PSLPKLVQ GPP+VL+VLLD
Sbjct: 509 LNHLTCTCRITSYFDSQGNNKDFFKIRMSILSVLVEVGMSPSLPKLVQAGPPEVLSVLLD 568

Query: 360 GRLVGYISSNEVEKVVAHLRRLKV 419
           GR+VG ISS+ +EKVVAHLRRLKV
Sbjct: 569 GRIVGSISSSVIEKVVAHLRRLKV 592

Query: 420  479
           
Sbjct: 629  592

Query: 480  539
           
Sbjct: 689  592

Query: 540  599
           
Sbjct: 749  592

Query: 600  659
           
Sbjct: 809  592

Query: 660  672
           
Sbjct: 869  592

[Close]

BLAST of MU62892 vs. TrEMBL
Match: W9SX99_9ROSA (DNA-directed RNA polymerase subunit beta OS=Morus notabilis GN=L484_008782 PE=3 SV=1)

HSP 1 Score: 136.7 bits (343), Expect = 4.2e-29
Identity = 66/81 (81.48%), Postives = 73/81 (90.12%), Query Frame = 1


Query: 1   VLLPGQLITLYLKEKLEDWLQKSKKLLEDEISNPSKNFEFCSLAHVKKVMDKNPSKQISS 60
           VLLPG LI +YLKEKLEDWL K+KKLL+DEI+N  K+F+FCSL  VKKVMDKNP+KQISS
Sbjct: 326 VLLPGHLIAIYLKEKLEDWLSKAKKLLQDEITNKGKSFDFCSLVQVKKVMDKNPAKQISS 385

Query: 61  AVENMLKTGRLVTQTGLDLQQ 120
           AVENMLKTGRL TQTGLDLQQ
Sbjct: 386 AVENMLKTGRLATQTGLDLQQ 406

Query: 121  180
           
Sbjct: 446  406

Query: 181  240
           
Sbjct: 506  406

Query: 241  244
           
Sbjct: 566  406

HSP 2 Score: 42.0 bits (97), Expect = 1.4e+00
Identity = 17/21 (80.95%), Postives = 19/21 (90.48%), Query Frame = 1


Query: 760 QIPDDLEVGYVPLSISGAYPG 819
           +IPDDLEVGYVPLS+ G YPG
Sbjct: 597 EIPDDLEVGYVPLSMGGTYPG 617

Query: 820  823
           
Sbjct: 657  617

HSP 3 Score: 250.8 bits (639), Expect = 2.0e-63
Identity = 120/150 (80.00%), Postives = 137/150 (91.33%), Query Frame = 3


Query: 240 AGFTVQAERLNFLRFVSHFRAVHRGASFAGLRTTTVRKLLPESWGFLCPVHTPDGEPCGL 299
           AG+TVQAERLNFLRF+S FRAVHRGASFAGLRTT+VRKLLPESWGFLCPVHTPDGEPCGL
Sbjct: 354 AGYTVQAERLNFLRFISFFRAVHRGASFAGLRTTSVRKLLPESWGFLCPVHTPDGEPCGL 413

Query: 300 LNHLTSTCRITSYFNSQGKVRDFYKIRTSILSVLIAVGMIPSLPKLVQMGPPDVLNVLLD 359
           LNH+TSTCR+ SY++S+G +RDF K+RTSIL VLI VGMIPSLPKLV+ GPP VL+VLLD
Sbjct: 414 LNHMTSTCRVASYYDSRGNIRDFSKMRTSILRVLIGVGMIPSLPKLVKSGPPAVLSVLLD 473

Query: 360 GRLVGYISSNEVEKVVAHLRRLKVLSPHVV 419
           GR+VG I S+E+EKVVAHLRRLKV +  V+
Sbjct: 474 GRVVGVIPSSEIEKVVAHLRRLKVSAASVI 503

Query: 420  479
           
Sbjct: 534  503

Query: 480  539
           
Sbjct: 594  503

Query: 540  599
           
Sbjct: 654  503

Query: 600  659
           
Sbjct: 714  503

Query: 660  690
           
Sbjct: 774  503

[Close]

BLAST of MU62892 vs. TrEMBL
Match: V4SW20_9ROSI (DNA-directed RNA polymerase subunit beta OS=Citrus clementina GN=CICLE_v10024768mg PE=3 SV=1)

HSP 1 Score: 125.6 bits (314), Expect = 9.6e-26
Identity = 58/81 (71.60%), Postives = 71/81 (87.65%), Query Frame = 1


Query: 1   VLLPGQLITLYLKEKLEDWLQKSKKLLEDEISNPSKNFEFCSLAHVKKVMDKNPSKQISS 60
           +LLPG LIT+YLKEKLEDWL+K KKL++DEI N  K F+F +LA++KKVM+KNPSKQI +
Sbjct: 272 ILLPGHLITIYLKEKLEDWLRKGKKLIQDEIENNGKKFDFFNLANIKKVMEKNPSKQIGT 331

Query: 61  AVENMLKTGRLVTQTGLDLQQ 120
           ++E MLKTGRL TQTGLDLQQ
Sbjct: 332 SIETMLKTGRLATQTGLDLQQ 352

Query: 121  180
           
Sbjct: 392  352

Query: 181  240
           
Sbjct: 452  352

Query: 241  244
           
Sbjct: 512  352

HSP 2 Score: 43.1 bits (100), Expect = 6.3e-01
Identity = 18/20 (90.00%), Postives = 19/20 (95.00%), Query Frame = 1


Query: 763 IPDDLEVGYVPLSISGAYPG 822
           IPDDLEVGYVPLS+ GAYPG
Sbjct: 503 IPDDLEVGYVPLSLGGAYPG 522

HSP 3 Score: 246.9 bits (629), Expect = 2.8e-62
Identity = 118/144 (81.94%), Postives = 133/144 (92.36%), Query Frame = 3


Query: 240 AGFTVQAERLNFLRFVSHFRAVHRGASFAGLRTTTVRKLLPESWGFLCPVHTPDGEPCGL 299
           AG+TVQAERLNFLRF+SHFRAVHRGASFAGLRTT+VRKLLPESWGFLCPVHTPDGEPCGL
Sbjct: 447 AGYTVQAERLNFLRFLSHFRAVHRGASFAGLRTTSVRKLLPESWGFLCPVHTPDGEPCGL 506

Query: 300 LNHLTSTCRITSYFNSQGKVRDFYKIRTSILSVLIAVGMIPSLPKLVQMGPPDVLNVLLD 359
           LNH+T TCRITS+F+S+G  RDF KIRTSIL+VLI +GM PSLPK+VQ GPPDVL+VLLD
Sbjct: 507 LNHMTCTCRITSFFDSKGNFRDFVKIRTSILNVLIGIGMTPSLPKIVQAGPPDVLSVLLD 566

Query: 360 GRLVGYISSNEVEKVVAHLRRLKV 419
           GR+VG I S EVEK V++LRRLK+
Sbjct: 567 GRVVGSIPSAEVEKAVSYLRRLKL 590

Query: 420  479
           
Sbjct: 627  590

Query: 480  539
           
Sbjct: 687  590

Query: 540  599
           
Sbjct: 747  590

Query: 600  659
           
Sbjct: 807  590

Query: 660  672
           
Sbjct: 867  590

[Close]

BLAST of MU62892 vs. TrEMBL
Match: A0A059BI44_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_G03213 PE=3 SV=1)

HSP 1 Score: 137.9 bits (346), Expect = 1.9e-29
Identity = 65/81 (80.25%), Postives = 75/81 (92.59%), Query Frame = 1


Query: 1   VLLPGQLITLYLKEKLEDWLQKSKKLLEDEISNPSKNFEFCSLAHVKKVMDKNPSKQISS 60
           VLLPG LIT+YLK+KLEDWLQK+KKLL+DE+ N +KNF+F SLAHVKKVMDKNP +QI S
Sbjct: 365 VLLPGHLITIYLKDKLEDWLQKAKKLLQDELKNKAKNFDFSSLAHVKKVMDKNPGRQIGS 424

Query: 61  AVENMLKTGRLVTQTGLDLQQ 120
           A+ENMLKTGRLVTQ+GLDLQQ
Sbjct: 425 AIENMLKTGRLVTQSGLDLQQ 445

Query: 121  180
           
Sbjct: 485  445

Query: 181  240
           
Sbjct: 545  445

Query: 241  244
           
Sbjct: 605  445

HSP 2 Score: 42.4 bits (98), Expect = 1.1e+00
Identity = 18/20 (90.00%), Postives = 18/20 (90.00%), Query Frame = 1


Query: 763 IPDDLEVGYVPLSISGAYPG 822
           IPDDLEVGYVPLS  GAYPG
Sbjct: 596 IPDDLEVGYVPLSFGGAYPG 615

HSP 3 Score: 246.9 bits (629), Expect = 2.8e-62
Identity = 118/144 (81.94%), Postives = 133/144 (92.36%), Query Frame = 3


Query: 240 AGFTVQAERLNFLRFVSHFRAVHRGASFAGLRTTTVRKLLPESWGFLCPVHTPDGEPCGL 299
           AG+TVQAERLNFLRF+SHFRAVHRGASFAGLRTT+VRKLLPESWGFLCPVHTPDGEPCGL
Sbjct: 447 AGYTVQAERLNFLRFLSHFRAVHRGASFAGLRTTSVRKLLPESWGFLCPVHTPDGEPCGL 506

Query: 300 LNHLTSTCRITSYFNSQGKVRDFYKIRTSILSVLIAVGMIPSLPKLVQMGPPDVLNVLLD 359
           LNH+T TCRITS+F+S+G  RDF KIRTSIL+VLI +GM PSLPK+VQ GPPDVL+VLLD
Sbjct: 507 LNHMTCTCRITSFFDSKGNFRDFVKIRTSILNVLIGIGMTPSLPKIVQAGPPDVLSVLLD 566

Query: 360 GRLVGYISSNEVEKVVAHLRRLKV 419
           GR+VG I S EVEK V++LRRLK+
Sbjct: 567 GRVVGSIPSAEVEKAVSYLRRLKL 590

Query: 420  479
           
Sbjct: 627  590

Query: 480  539
           
Sbjct: 687  590

Query: 540  599
           
Sbjct: 747  590

Query: 600  659
           
Sbjct: 807  590

Query: 660  672
           
Sbjct: 867  590

[Close]

BLAST of MU62892 vs. TAIR10
Match: AT1G29940.1 (AT1G29940.1 nuclear RNA polymerase A2)

HSP 1 Score: 232.3 bits (591), Expect = 3.7e-61
Identity = 111/150 (74.00%), Postives = 129/150 (86.00%), Query Frame = 3


Query: 240 AGFTVQAERLNFLRFVSHFRAVHRGASFAGLRTTTVRKLLPESWGFLCPVHTPDGEPCGL 299
           AG+TVQAERLNFLRF+S FRAVHRGASFAGLRTTTVRKLLPESWGFLCPVHTPDG PCGL
Sbjct: 453 AGYTVQAERLNFLRFLSFFRAVHRGASFAGLRTTTVRKLLPESWGFLCPVHTPDGTPCGL 512

Query: 300 LNHLTSTCRITSYFNSQGKVRDFYKIRTSILSVLIAVGMIPSLPKLVQMGPPDVLNVLLD 359
           LNH+T T RITS F+S+G +RDF KIR S++ VL   GM+PSLPKLV+ GPP V++VLLD
Sbjct: 513 LNHMTRTSRITSQFDSKGNIRDFLKIRKSVVDVLTGAGMVPSLPKLVRAGPPKVIHVLLD 572

Query: 360 GRLVGYISSNEVEKVVAHLRRLKVLSPHVV 419
           G++VG +SSN V KVV+++RRLKV +P V+
Sbjct: 573 GQVVGTLSSNLVTKVVSYIRRLKVEAPSVI 602

Query: 420  479
           
Sbjct: 633  602

Query: 480  539
           
Sbjct: 693  602

Query: 540  599
           
Sbjct: 753  602

Query: 600  659
           
Sbjct: 813  602

Query: 660  690
           
Sbjct: 873  602

HSP 2 Score: 103.2 bits (256), Expect = 2.6e-22
Identity = 47/81 (58.02%), Postives = 63/81 (77.78%), Query Frame = 1


Query: 1   VLLPGQLITLYLKEKLEDWLQKSKKLLEDEISNPSKNFEFCSLAHVKKVMDKNPSKQISS 60
           +L+PG +IT+YLKEKLE+WL+K K LL+DE+ N +  F F SLA VKK+++KNP + I +
Sbjct: 371 ILVPGHVITIYLKEKLEEWLRKCKSLLKDELDNTNSKFSFESLADVKKLINKNPPRSIGT 430

Query: 61  AVENMLKTGRLVTQTGLDLQQ 120
           ++E +LKTG L TQ+GLDLQQ
Sbjct: 431 SIETLLKTGALKTQSGLDLQQ 451

Query: 121  180
           
Sbjct: 491  451

Query: 181  240
           
Sbjct: 551  451

Query: 241  244
           
Sbjct: 611  451

HSP 3 Score: 38.1 bits (87), Expect = 1.0e-02
Identity = 15/20 (75.00%), Postives = 16/20 (80.00%), Query Frame = 1


Query: 763 IPDDLEVGYVPLSISGAYPG 822
           IP+DLEVGYVP S+ G+YPG
Sbjct: 602 IPEDLEVGYVPTSMGGSYPG 621

[Close]

BLAST of MU62892 vs. TAIR10
Match: AT5G45140.1 (AT5G45140.1 nuclear RNA polymerase C2)

HSP 1 Score: 52.4 bits (124), Expect = 5.2e-07
Identity = 34/107 (31.78%), Postives = 48/107 (44.86%), Query Frame = 3


Query: 348 RKLLPESWGFLCPVHTPDGEPCGLLNHLTSTCRITSYFNSQGKVRDFYKIRTSILSVLIA 407
           R L P  WG LCP  TP+GE CGL+ +L     +T+       V   YK+  + L VL A
Sbjct: 480 RSLQPSQWGMLCPCDTPEGESCGLVKNLALMTHVTTDEEEGPLVAMCYKLGVTDLEVLSA 539

Query: 408 VGMIPSLPKLVQMGPPDVLNVLLDGRLVGYISSNEVEKVVAHLRRLK 467
                      ++  PD   V+L+G ++G    +  +     LRRL+
Sbjct: 540 ----------EELHTPDSFLVILNGLILG--KHSRPQYFANSLRRLR 574

Query: 468  527
           
Sbjct: 600  574

Query: 528  587
           
Sbjct: 660  574

Query: 588  647
           
Sbjct: 720  574

Query: 648  669
           
Sbjct: 780  574

[Close]

BLAST of MU62892 vs. NCBI nr
Match: gi|659089195|ref|XP_008445376.1| (PREDICTED: DNA-directed RNA polymerase I subunit RPA2 [Cucumis melo])

HSP 1 Score: 304.3 bits (778), Expect = 2.2e-79
Identity = 149/150 (99.33%), Postives = 150/150 (100.00%), Query Frame = 3


Query: 240 AGFTVQAERLNFLRFVSHFRAVHRGASFAGLRTTTVRKLLPESWGFLCPVHTPDGEPCGL 299
           AGFTVQAERLNFLRFVSHFRAVHRGASFAGLRTTTVRKLLPESWGFLCPVHTPDGEPCGL
Sbjct: 447 AGFTVQAERLNFLRFVSHFRAVHRGASFAGLRTTTVRKLLPESWGFLCPVHTPDGEPCGL 506

Query: 300 LNHLTSTCRITSYFNSQGKVRDFYKIRTSILSVLIAVGMIPSLPKLVQMGPPDVLNVLLD 359
           LNHLTSTCRITSYFNSQGKVRDFYKIRTSILSVLIAVGMIPSLPKLVQMGPPDVLNVLLD
Sbjct: 507 LNHLTSTCRITSYFNSQGKVRDFYKIRTSILSVLIAVGMIPSLPKLVQMGPPDVLNVLLD 566

Query: 360 GRLVGYISSNEVEKVVAHLRRLKVLSPHVV 419
           GRLVGYISSNEVEKVVAHLRRLKVLSPHV+
Sbjct: 567 GRLVGYISSNEVEKVVAHLRRLKVLSPHVI 596

Query: 420  479
           
Sbjct: 627  596

Query: 480  539
           
Sbjct: 687  596

Query: 540  599
           
Sbjct: 747  596

Query: 600  659
           
Sbjct: 807  596

Query: 660  690
           
Sbjct: 867  596

[Close]

BLAST of MU62892 vs. NCBI nr
Match: gi|449458081|ref|XP_004146776.1| (PREDICTED: DNA-directed RNA polymerase I subunit RPA2 [Cucumis sativus])

HSP 1 Score: 295.4 bits (755), Expect = 1.0e-76
Identity = 144/150 (96.00%), Postives = 148/150 (98.67%), Query Frame = 3


Query: 240 AGFTVQAERLNFLRFVSHFRAVHRGASFAGLRTTTVRKLLPESWGFLCPVHTPDGEPCGL 299
           AGFTVQAERLNFLRFVSHFRAVHRGASFAGLRTTTVRKLLPESWGFLCPVHTPDGEPCGL
Sbjct: 447 AGFTVQAERLNFLRFVSHFRAVHRGASFAGLRTTTVRKLLPESWGFLCPVHTPDGEPCGL 506

Query: 300 LNHLTSTCRITSYFNSQGKVRDFYKIRTSILSVLIAVGMIPSLPKLVQMGPPDVLNVLLD 359
           LNHLTSTCRITS+FNSQGKVRDFYKIRTSILSVLIAVGMIPSLPKLVQMG PD LNVLLD
Sbjct: 507 LNHLTSTCRITSHFNSQGKVRDFYKIRTSILSVLIAVGMIPSLPKLVQMGLPDFLNVLLD 566

Query: 360 GRLVGYISSNEVEKVVAHLRRLKVLSPHVV 419
           GR+VGYISSNEVEK+VAHLRRLKVLSPHV+
Sbjct: 567 GRIVGYISSNEVEKIVAHLRRLKVLSPHVI 596

Query: 420  479
           
Sbjct: 627  596

Query: 480  539
           
Sbjct: 687  596

Query: 540  599
           
Sbjct: 747  596

Query: 600  659
           
Sbjct: 807  596

Query: 660  690
           
Sbjct: 867  596

[Close]

BLAST of MU62892 vs. NCBI nr
Match: gi|700192552|gb|KGN47756.1| (hypothetical protein Csa_6G400300 [Cucumis sativus])

HSP 1 Score: 295.4 bits (755), Expect = 1.0e-76
Identity = 144/150 (96.00%), Postives = 148/150 (98.67%), Query Frame = 3


Query: 240 AGFTVQAERLNFLRFVSHFRAVHRGASFAGLRTTTVRKLLPESWGFLCPVHTPDGEPCGL 299
           AGFTVQAERLNFLRFVSHFRAVHRGASFAGLRTTTVRKLLPESWGFLCPVHTPDGEPCGL
Sbjct: 527 AGFTVQAERLNFLRFVSHFRAVHRGASFAGLRTTTVRKLLPESWGFLCPVHTPDGEPCGL 586

Query: 300 LNHLTSTCRITSYFNSQGKVRDFYKIRTSILSVLIAVGMIPSLPKLVQMGPPDVLNVLLD 359
           LNHLTSTCRITS+FNSQGKVRDFYKIRTSILSVLIAVGMIPSLPKLVQMG PD LNVLLD
Sbjct: 587 LNHLTSTCRITSHFNSQGKVRDFYKIRTSILSVLIAVGMIPSLPKLVQMGLPDFLNVLLD 646

Query: 360 GRLVGYISSNEVEKVVAHLRRLKVLSPHVV 419
           GR+VGYISSNEVEK+VAHLRRLKVLSPHV+
Sbjct: 647 GRIVGYISSNEVEKIVAHLRRLKVLSPHVI 676

Query: 420  479
           
Sbjct: 707  676

Query: 480  539
           
Sbjct: 767  676

Query: 540  599
           
Sbjct: 827  676

Query: 600  659
           
Sbjct: 887  676

Query: 660  690
           
Sbjct: 947  676

[Close]

BLAST of MU62892 vs. NCBI nr
Match: gi|703157633|ref|XP_010111778.1| (putative DNA-directed RNA polymerase I subunit RPA2 [Morus notabilis])

HSP 1 Score: 254.6 bits (649), Expect = 2.0e-64
Identity = 122/144 (84.72%), Postives = 134/144 (93.06%), Query Frame = 3


Query: 240 AGFTVQAERLNFLRFVSHFRAVHRGASFAGLRTTTVRKLLPESWGFLCPVHTPDGEPCGL 299
           AGFTVQAERLN+LRF+SHFRAVHRG SFAGLRTT+VRKLLPESWGFLCPVHTPDGEPCGL
Sbjct: 449 AGFTVQAERLNYLRFLSHFRAVHRGTSFAGLRTTSVRKLLPESWGFLCPVHTPDGEPCGL 508

Query: 300 LNHLTSTCRITSYFNSQGKVRDFYKIRTSILSVLIAVGMIPSLPKLVQMGPPDVLNVLLD 359
           LNHLT TCRITSYF+SQG  +DF+KIR SILSVL+ VGM PSLPKLVQ GPP+VL+VLLD
Sbjct: 509 LNHLTCTCRITSYFDSQGNNKDFFKIRMSILSVLVEVGMSPSLPKLVQAGPPEVLSVLLD 568

Query: 360 GRLVGYISSNEVEKVVAHLRRLKV 419
           GR+VG ISS+ +EKVVAHLRRLKV
Sbjct: 569 GRIVGSISSSVIEKVVAHLRRLKV 592

Query: 420  479
           
Sbjct: 629  592

Query: 480  539
           
Sbjct: 689  592

Query: 540  599
           
Sbjct: 749  592

Query: 600  659
           
Sbjct: 809  592

Query: 660  672
           
Sbjct: 869  592

[Close]

BLAST of MU62892 vs. NCBI nr
Match: gi|568819498|ref|XP_006464288.1| (PREDICTED: DNA-directed RNA polymerase I subunit 2 isoform X2 [Citrus sinensis])

HSP 1 Score: 252.3 bits (643), Expect = 9.7e-64
Identity = 120/150 (80.00%), Postives = 137/150 (91.33%), Query Frame = 3


Query: 240 AGFTVQAERLNFLRFVSHFRAVHRGASFAGLRTTTVRKLLPESWGFLCPVHTPDGEPCGL 299
           AG+TVQAERLNFLRF+S FRAVHRGASFAGLRTT+VRKLLPESWGFLCPVHTPDGEPCGL
Sbjct: 447 AGYTVQAERLNFLRFISFFRAVHRGASFAGLRTTSVRKLLPESWGFLCPVHTPDGEPCGL 506

Query: 300 LNHLTSTCRITSYFNSQGKVRDFYKIRTSILSVLIAVGMIPSLPKLVQMGPPDVLNVLLD 359
           LNH+TSTCR+ SY++S+G +RDF K+RTSIL VLI VGMIPSLPKLV+ GPP VL+VLLD
Sbjct: 507 LNHMTSTCRVASYYDSRGNIRDFSKMRTSILRVLIGVGMIPSLPKLVKSGPPAVLSVLLD 566

Query: 360 GRLVGYISSNEVEKVVAHLRRLKVLSPHVV 419
           GR+VG I S+E+EKVVAHLRRLKV +  V+
Sbjct: 567 GRVVGVIPSSEIEKVVAHLRRLKVSAASVI 596

Query: 420  479
           
Sbjct: 627  596

Query: 480  539
           
Sbjct: 687  596

Query: 540  599
           
Sbjct: 747  596

Query: 600  659
           
Sbjct: 807  596

Query: 660  690
           
Sbjct: 867  596

The following BLAST results are available for this feature:

BLAST of MU62892 vs. Swiss-Prot


Match Name	E-value	Identity	Description
NRPA2_ARATH	6.5e-60	74.00	DNA-directed RNA polymerase I subunit 2 OS=Arabidopsis thaliana GN=NRPA2 PE=2 SV...	[more]
RPA2_DICDI	2.6e-32	49.32	DNA-directed RNA polymerase I subunit rpa2 OS=Dictyostelium discoideum GN=polr1b...	[more]
RPA2_MOUSE	3.4e-32	50.34	DNA-directed RNA polymerase I subunit RPA2 OS=Mus musculus GN=Polr1b PE=1 SV=2	[more]
RPA2_RAT	4.4e-32	50.34	DNA-directed RNA polymerase I subunit RPA2 OS=Rattus norvegicus GN=Polr1b PE=1 S...	[more]
RPA2_YEAST	4.9e-31	47.62	DNA-directed RNA polymerase I subunit RPA135 OS=Saccharomyces cerevisiae (strain...	[more]

BLAST of MU62892 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KIZ8_CUCSA	2.0e-76	96.00	DNA-directed RNA polymerase subunit beta OS=Cucumis sativus GN=Csa_6G400300 PE=3...	[more]
A0A0A0KIZ8_CUCSA	4.6e-36	94.12	DNA-directed RNA polymerase subunit beta OS=Cucumis sativus GN=Csa_6G400300 PE=3...	[more]
W9SX99_9ROSA	4.2e-29	81.48	DNA-directed RNA polymerase subunit beta OS=Morus notabilis GN=L484_008782 PE=3 ...	[more]
V4SW20_9ROSI	9.6e-26	71.60	DNA-directed RNA polymerase subunit beta OS=Citrus clementina GN=CICLE_v10024768...	[more]
A0A059BI44_EUCGR	1.9e-29	80.25	Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_G03213 PE=3 SV=1	[more]

BLAST of MU62892 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G29940.1	3.7e-61	74.00	nuclear RNA polymerase A2	[more]
AT5G45140.1	5.2e-07	31.78	nuclear RNA polymerase C2	[more]

BLAST of MU62892 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659089195\|ref\|XP_008445376.1\|	2.2e-79	99.33	PREDICTED: DNA-directed RNA polymerase I subunit RPA2 [Cucumis melo]	[more]
gi\|449458081\|ref\|XP_004146776.1\|	1.0e-76	96.00	PREDICTED: DNA-directed RNA polymerase I subunit RPA2 [Cucumis sativus]	[more]
gi\|700192552\|gb\|KGN47756.1\|	1.0e-76	96.00	hypothetical protein Csa_6G400300 [Cucumis sativus]	[more]
gi\|703157633\|ref\|XP_010111778.1\|	2.0e-64	84.72	putative DNA-directed RNA polymerase I subunit RPA2 [Morus notabilis]	[more]
gi\|568819498\|ref\|XP_006464288.1\|	9.7e-64	80.00	PREDICTED: DNA-directed RNA polymerase I subunit 2 isoform X2 [Citrus sinensis]	[more]

Annotated Terms

The following terms have been associated with this transcribed_cluster:

Vocabulary: **INTERPRO**
Term	Definition
IPR007645	RNA_pol_Rpb2_3
IPR015712	DNA-dir_RNA_pol_su2

Vocabulary: **Biological Process**
Term	Definition
GO:0006351	transcription, DNA-templated

Vocabulary: **Molecular Function**
Term	Definition
GO:0003677	DNA binding
GO:0003899	DNA-directed RNA polymerase activity
GO:0032549	ribonucleoside binding

GO Annotation

GO Assignments

This transcribed_cluster is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006351	transcription, DNA-templated
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0003899	DNA-directed RNA polymerase activity
molecular_function	GO:0032549	ribonucleoside binding

Relationships

This transcribed_cluster is associated with the following gene feature(s):


Feature Name	Unique Name	Type
MELO3C011382	MELO3C011382	gene
MELO3C011383	MELO3C011383	gene

The following EST feature(s) are a part of this transcribed_cluster:


Feature Name	Unique Name	Type
PEa0004I01	PEa0004I01	EST

InterPro

Analysis Name: InterPro Annotations of melon unigene v4
Date Performed: 2016-11-16


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR007645	RNA polymerase Rpb2, domain 3	PFAM	PF04565	RNA_pol_Rpb2_3	coord: 87..150 score: 6.0
IPR015712	DNA-directed RNA polymerase, subunit 2	PANTHER	PTHR20856	DNA-DIRECTED RNA POLYMERASE I SUBUNIT 2	coord: 1..217 score: 1.5
None	No IPR available	GENE3D	G3DSA:3.90.1100.10		coord: 3..172 score: 5.6
None	No IPR available	PANTHER	PTHR20856:SF5	DNA-DIRECTED RNA POLYMERASE I SUBUNIT RPA2	coord: 1..217 score: 1.5
None	No IPR available	unknown	SSF64484	beta and beta-prime subunits of DNA dependent RNA-polymerase	coord: 1..209 score: 1.19