CU163540 (transcribed_cluster) Cucumber (Chinese Long) v2 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CU163540
Type	transcribed_cluster
Organism	Cucumis. sativus (Cucumber (Chinese Long) v2)
Description	Pectin lyase superfamily protein
Location	Chr3 : 3620005 .. 3620216 (-)
Sequence length	212

Genome Viewer

Sequences

The following sequences are available for this feature:

transcribed_cluster sequence

AATACACCAGTGGCAGTAGATTTTGAATGCAGCCAAGTTCTTCCATGTCAAGGCATAGTGTTACAAGACATTAACCTTACTTTCAATGGTGGTGGGAAGACAACTTCCATTTGTCATAATGTTAAGGGTTCTGCATCTGGTCAACAATTACCTCCCTCTTGCCTTTAATTACTTTGTGTTCTTTCTTTTCTTTTCTTCCTCACTACAGAGAG

Library


Library category	ESTs
hermaphrodite flower library	1

Homology

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BLAST of CU163540 vs. Swiss-Prot
Match: PGLR_ACTDE (Polygalacturonase OS=Actinidia deliciosa PE=2 SV=1)

HSP 1 Score: 52.0 bits (123), Expect = 3.1e-06
Identity = 21/44 (47.73%), Postives = 30/44 (68.18%), Query Frame = 1


Query: 10  VAVDFECSQVLPCQGIVLQDINLTFNGGGKTTSICHNVKGSASG 69
           VA+ F+CS+  PCQGIVL+D++L   GG    ++C+NV+ S +G
Sbjct: 406 VAITFDCSKRFPCQGIVLEDVDLEIEGGAAAKALCNNVELSETG 449

Query: 70   129
           
Sbjct: 466  449

Query: 130  142
           
Sbjct: 526  449

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BLAST of CU163540 vs. Swiss-Prot
Match: PGLR_OENOR (Exopolygalacturonase (Fragment) OS=Oenothera organensis PE=2 SV=1)

HSP 1 Score: 50.4 bits (119), Expect = 9.0e-06
Identity = 23/49 (46.94%), Postives = 30/49 (61.22%), Query Frame = 1


Query: 4   TPVAVDFECSQVLPCQGIVLQDINLTFNG-GGKTTSICHNVKGSASGQQ 63
           T  AV   CS+  PC G+ L DI+LT++G GG  TS+C N+K +  G+Q
Sbjct: 301 TKEAVKLVCSKSFPCNGVELADIDLTYSGKGGPATSVCENIKPTIKGKQ 349

Query: 64   123
           
Sbjct: 361  349

Query: 124  148
           
Sbjct: 421  349

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BLAST of CU163540 vs. TrEMBL
Match: A0A0A0L660_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G063620 PE=3 SV=1)

HSP 1 Score: 105.1 bits (261), Expect = 3.4e-20
Identity = 49/49 (100.00%), Postives = 49/49 (100.00%), Query Frame = 1


Query: 1   NTPVAVDFECSQVLPCQGIVLQDINLTFNGGGKTTSICHNVKGSASGQQ 60
           NTPVAVDFECSQVLPCQGIVLQDINLTFNGGGKTTSICHNVKGSASGQQ
Sbjct: 192 NTPVAVDFECSQVLPCQGIVLQDINLTFNGGGKTTSICHNVKGSASGQQ 240

Query: 61   120
           
Sbjct: 252  240

Query: 121  148
           
Sbjct: 312  240

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BLAST of CU163540 vs. TrEMBL
Match: A0A0A0L7H9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G063610 PE=3 SV=1)

HSP 1 Score: 88.2 bits (217), Expect = 4.3e-15
Identity = 40/49 (81.63%), Postives = 44/49 (89.80%), Query Frame = 1


Query: 1   NTPVAVDFECSQVLPCQGIVLQDINLTFNGGGKTTSICHNVKGSASGQQ 60
           NT VAVDFECS+V+PCQGIVLQDINL FNGGG+TTS CHNV+GSA G Q
Sbjct: 306 NTQVAVDFECSEVVPCQGIVLQDINLPFNGGGRTTSNCHNVRGSAFGHQ 354

Query: 61   120
           
Sbjct: 366  354

Query: 121  148
           
Sbjct: 426  354

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BLAST of CU163540 vs. NCBI nr
Match: gi|700200929|gb|KGN56062.1| (hypothetical protein Csa_3G063620 [Cucumis sativus])

HSP 1 Score: 106.3 bits (264), Expect = 2.2e-20
Identity = 49/49 (100.00%), Postives = 49/49 (100.00%), Query Frame = 1


Query: 1   NTPVAVDFECSQVLPCQGIVLQDINLTFNGGGKTTSICHNVKGSASGQQ 60
           NTPVAVDFECSQVLPCQGIVLQDINLTFNGGGKTTSICHNVKGSASGQQ
Sbjct: 192 NTPVAVDFECSQVLPCQGIVLQDINLTFNGGGKTTSICHNVKGSASGQQ 240

Query: 61   120
           
Sbjct: 252  240

Query: 121  148
           
Sbjct: 312  240

[Close]

BLAST of CU163540 vs. NCBI nr
Match: gi|449463753|ref|XP_004149596.1| (PREDICTED: exopolygalacturonase-like [Cucumis sativus])

HSP 1 Score: 106.3 bits (264), Expect = 2.2e-20
Identity = 49/49 (100.00%), Postives = 49/49 (100.00%), Query Frame = 1


Query: 1   NTPVAVDFECSQVLPCQGIVLQDINLTFNGGGKTTSICHNVKGSASGQQ 60
           NTPVAVDFECSQVLPCQGIVLQDINLTFNGGGKTTSICHNVKGSASGQQ
Sbjct: 295 NTPVAVDFECSQVLPCQGIVLQDINLTFNGGGKTTSICHNVKGSASGQQ 343

Query: 61   120
           
Sbjct: 355  343

Query: 121  148
           
Sbjct: 415  343

[Close]

BLAST of CU163540 vs. NCBI nr
Match: gi|778688362|ref|XP_011652730.1| (PREDICTED: exopolygalacturonase-like [Cucumis sativus])

HSP 1 Score: 89.4 bits (220), Expect = 2.8e-15
Identity = 40/49 (81.63%), Postives = 44/49 (89.80%), Query Frame = 1


Query: 1   NTPVAVDFECSQVLPCQGIVLQDINLTFNGGGKTTSICHNVKGSASGQQ 60
           NT VAVDFECS+V+PCQGIVLQDINL FNGGG+TTS CHNV+GSA G Q
Sbjct: 306 NTQVAVDFECSEVVPCQGIVLQDINLPFNGGGRTTSNCHNVRGSAFGHQ 354

Query: 61   120
           
Sbjct: 366  354

Query: 121  148
           
Sbjct: 426  354

[Close]

BLAST of CU163540 vs. NCBI nr
Match: gi|700200928|gb|KGN56061.1| (hypothetical protein Csa_3G063610 [Cucumis sativus])

HSP 1 Score: 89.4 bits (220), Expect = 2.8e-15
Identity = 40/49 (81.63%), Postives = 44/49 (89.80%), Query Frame = 1


Query: 1   NTPVAVDFECSQVLPCQGIVLQDINLTFNGGGKTTSICHNVKGSASGQQ 60
           NT VAVDFECS+V+PCQGIVLQDINL FNGGG+TTS CHNV+GSA G Q
Sbjct: 306 NTQVAVDFECSEVVPCQGIVLQDINLPFNGGGRTTSNCHNVRGSAFGHQ 354

Query: 61   120
           
Sbjct: 366  354

Query: 121  148
           
Sbjct: 426  354

[Close]

BLAST of CU163540 vs. NCBI nr
Match: gi|659096624|ref|XP_008449197.1| (PREDICTED: exopolygalacturonase-like [Cucumis melo])

HSP 1 Score: 89.0 bits (219), Expect = 3.6e-15
Identity = 40/49 (81.63%), Postives = 43/49 (87.76%), Query Frame = 1


Query: 1   NTPVAVDFECSQVLPCQGIVLQDINLTFNGGGKTTSICHNVKGSASGQQ 60
           NT VAVDFECSQV PCQGIVLQDINL FNGGG+TT+ CHNV+GSA G Q
Sbjct: 301 NTQVAVDFECSQVFPCQGIVLQDINLPFNGGGRTTANCHNVRGSAFGHQ 349

Query: 61   120
           
Sbjct: 361  349

Query: 121  148
           
Sbjct: 421  349

The following BLAST results are available for this feature:

BLAST of CU163540 vs. Swiss-Prot


Match Name	E-value	Identity	Description
PGLR_ACTDE	3.1e-06	47.73	Polygalacturonase OS=Actinidia deliciosa PE=2 SV=1	[more]
PGLR_OENOR	9.0e-06	46.94	Exopolygalacturonase (Fragment) OS=Oenothera organensis PE=2 SV=1	[more]

BLAST of CU163540 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L660_CUCSA	3.4e-20	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G063620 PE=3 SV=1	[more]
A0A0A0L7H9_CUCSA	4.3e-15	81.63	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G063610 PE=3 SV=1	[more]

BLAST of CU163540 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700200929\|gb\|KGN56062.1\|	2.2e-20	100.00	hypothetical protein Csa_3G063620 [Cucumis sativus]	[more]
gi\|449463753\|ref\|XP_004149596.1\|	2.2e-20	100.00	PREDICTED: exopolygalacturonase-like [Cucumis sativus]	[more]
gi\|778688362\|ref\|XP_011652730.1\|	2.8e-15	81.63	PREDICTED: exopolygalacturonase-like [Cucumis sativus]	[more]
gi\|700200928\|gb\|KGN56061.1\|	2.8e-15	81.63	hypothetical protein Csa_3G063610 [Cucumis sativus]	[more]
gi\|659096624\|ref\|XP_008449197.1\|	3.6e-15	81.63	PREDICTED: exopolygalacturonase-like [Cucumis melo]	[more]

Annotated Terms

The following terms have been associated with this transcribed_cluster:

Vocabulary: **INTERPRO**
Term	Definition
IPR000743	Glyco_hydro_28
IPR011050	Pectin_lyase_fold/virulence
IPR012334	Pectin_lyas_fold

Vocabulary: **Molecular Function**
Term	Definition
GO:0004650	polygalacturonase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0005975	carbohydrate metabolic process

GO Annotation

GO Assignments

This transcribed_cluster is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0004650	polygalacturonase activity

Relationships

This transcribed_cluster is associated with the following gene feature(s):


Feature Name	Unique Name	Type
Csa3G063620	Csa3G063620	gene

The following EST feature(s) are a part of this transcribed_cluster:


Feature Name	Unique Name	Type
H0073647	H0073647	EST

InterPro

Analysis Name: InterPro Annotations of cucumber unigene v3
Date Performed: 2016-11-16


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000743	Glycoside hydrolase, family 28	PFAM	PF00295	Glyco_hydro_28	coord: 3..45 score: 9.
IPR011050	Pectin lyase fold/virulence factor	unknown	SSF51126	Pectin lyase-like	coord: 2..55 score: 3.84
IPR012334	Pectin lyase fold	GENE3D	G3DSA:2.160.20.10		coord: 2..54 score: 3.4
None	No IPR available	PANTHER	PTHR31375	FAMILY NOT NAMED	coord: 1..55 score: 5.3