CU120115 (transcribed_cluster) Cucumber (Chinese Long) v2 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CU120115
Type	transcribed_cluster
Organism	Cucumis. sativus (Cucumber (Chinese Long) v2)
Description	Unknown protein
Location	Chr1 : 2880333 .. 2881708 (-)
Sequence length	409

Genome Viewer

Sequences

The following sequences are available for this feature:

transcribed_cluster sequence

CAACTTCCCTTCTAGTTTCCTAAGTAGTTGGTTAACCCTTCGTTATACTAAATGTTTGAGGTTTATCAGCCGACACAAGCTCCTCGTCGTTTGGACTGAGGACCAAATTATCATGAATCCCGTCCACACATTTTTCACCATTTGAGGCTTCTATTTTAACAAAATTTTCCTCTTCTCTATTTTCTACATTTTCCCAAGTTGTAGAATCTGGCTCCATGTTCTCTCCATCATTCAGAGGAAGAATATATAGATATCGTCTCCATTTAGCAGAGAAGTTTGGATGGAAGATTCGAGAGACCTGAGAGACAGAGGATATTCTGAGTTTTCCAGGAGCTGCACTATTGATGGAATCTTCAATGTCTTGAGGTTTAACATCGGTACGCCAAGTATAAAATGAGCAAACTTGTCG

Library


Library category	ESTs
fruit (8DPP) library	1
gynoecious flower library	1

Homology

[Close]

BLAST of CU120115 vs. TrEMBL
Match: A0A0A0LV59_CUCSA (tRNA pseudouridine synthase OS=Cucumis sativus GN=Csa_1G025830 PE=3 SV=1)

HSP 1 Score: 229.9 bits (585), Expect = 1.8e-57
Identity = 111/124 (89.52%), Postives = 113/124 (91.13%), Query Frame = -1


Query: 38  RQVCSFYTWRTDVKPQDIEDSINSAAPGKLRISSVSQVSRIFHPNFSAKWRRYLYILPLN 97
           RQVCSFYTWRTDVKPQDIEDSINSAAPGKLR SSVSQVSRIFHPNFSAKWRRYLYILPLN
Sbjct: 188 RQVCSFYTWRTDVKPQDIEDSINSAAPGKLRTSSVSQVSRIFHPNFSAKWRRYLYILPLN 247

Query: 98  DGENMEPDSTTWXXXXXXXXXXFVKIEASNGEKCVDGIHDNLVLSPNDEELVSADKPQTF 157
           DGENMEPDSTTW          FVKIEASNGEKCVDGIHDNLVLSPND+ELVSADKPQTF
Sbjct: 248 DGENMEPDSTTWENVENREEENFVKIEASNGEKCVDGIHDNLVLSPNDQELVSADKPQTF 307

Query: 158 SITK 217
           SIT+
Sbjct: 308 SITR 311

Query: 218  277
           
Sbjct: 368  311

Query: 278  337
           
Sbjct: 428  311

Query: 338  397
           
Sbjct: 488  311

Query: 398  410
           
Sbjct: 548  311

[Close]

BLAST of CU120115 vs. TrEMBL
Match: U5FKW5_POPTR (tRNA pseudouridine synthase OS=Populus trichocarpa GN=POPTR_0018s14400g PE=3 SV=1)

HSP 1 Score: 126.3 bits (316), Expect = 2.8e-26
Identity = 63/124 (50.81%), Postives = 81/124 (65.32%), Query Frame = -1


Query: 38  RQVCSFYTWRTDVKPQDIEDSINSAAPGKLRISSVSQVSRIFHPNFSAKWRRYLYILPLN 97
           +QVCSFYTWR DVKP +IED+IN  APGK+R+ S+S+VSR FHPNFSAKWRRYLYI PLN
Sbjct: 204 QQVCSFYTWRKDVKPHEIEDAINDVAPGKVRVESISEVSRAFHPNFSAKWRRYLYIFPLN 263

Query: 98  DGENMEPDSTTWXXXXXXXXXXFVKIEASNGEKCVDGIHDNLVLSPNDEELVSADKPQTF 157
           DGEN E                  K     GE   +   +N ++S +++EL  A KP++F
Sbjct: 264 DGENREEIEGEGGIENFSSHENCEKQRNECGELASEENVENSIIS-DEDELQGAKKPRSF 323

Query: 158 SITK 217
           S+ +
Sbjct: 324 SVCR 326

Query: 218  277
           
Sbjct: 384  326

Query: 278  337
           
Sbjct: 444  326

Query: 338  397
           
Sbjct: 504  326

Query: 398  410
           
Sbjct: 564  326

[Close]

BLAST of CU120115 vs. TrEMBL
Match: U5FJE5_POPTR (tRNA pseudouridine synthase OS=Populus trichocarpa GN=POPTR_0018s14400g PE=3 SV=1)

HSP 1 Score: 126.3 bits (316), Expect = 2.8e-26
Identity = 63/124 (50.81%), Postives = 81/124 (65.32%), Query Frame = -1


Query: 38  RQVCSFYTWRTDVKPQDIEDSINSAAPGKLRISSVSQVSRIFHPNFSAKWRRYLYILPLN 97
           +QVCSFYTWR DVKP +IED+IN  APGK+R+ S+S+VSR FHPNFSAKWRRYLYI PLN
Sbjct: 195 QQVCSFYTWRKDVKPHEIEDAINDVAPGKVRVESISEVSRAFHPNFSAKWRRYLYIFPLN 254

Query: 98  DGENMEPDSTTWXXXXXXXXXXFVKIEASNGEKCVDGIHDNLVLSPNDEELVSADKPQTF 157
           DGEN E                  K     GE   +   +N ++S +++EL  A KP++F
Sbjct: 255 DGENREEIEGEGGIENFSSHENCEKQRNECGELASEENVENSIIS-DEDELQGAKKPRSF 314

Query: 158 SITK 217
           S+ +
Sbjct: 315 SVCR 317

Query: 218  277
           
Sbjct: 375  317

Query: 278  337
           
Sbjct: 435  317

Query: 338  397
           
Sbjct: 495  317

Query: 398  410
           
Sbjct: 555  317

[Close]

BLAST of CU120115 vs. TrEMBL
Match: V4UJT6_9ROSI (tRNA pseudouridine synthase OS=Citrus clementina GN=CICLE_v10008288mg PE=3 SV=1)

HSP 1 Score: 124.8 bits (312), Expect = 8.1e-26
Identity = 69/125 (55.20%), Postives = 84/125 (67.20%), Query Frame = -1


Query: 38  RQVCSFYTWRTDVKPQDIEDSINSAAPGKLRISSVSQVSRIFHPNFSAKWRRYLYILPLN 97
           +QVCSFYTWR DVKP +IED+INSAAPGK+R+ SVSQVSR+FHPNFSAKWRRYLYI PLN
Sbjct: 196 QQVCSFYTWRKDVKPSEIEDAINSAAPGKIRVISVSQVSRVFHPNFSAKWRRYLYIFPLN 255

Query: 98  DGENMEPDSTTWXXXXXXXXXXFVKIEASNGEKCVDGIHDNLVLSPNDE-ELVSADKPQT 157
           DGE  E    +            V  + SNG  C + I + L+   NDE    S +KP+ 
Sbjct: 256 DGEKREQSIDSEVEVENFHTSNNVG-KQSNG--CYENIENLLI---NDEGGFGSHEKPRN 314

Query: 158 FSITK 217
           F+I +
Sbjct: 316 FTICR 314

Query: 218  277
           
Sbjct: 376  314

Query: 278  337
           
Sbjct: 436  314

Query: 338  397
           
Sbjct: 496  314

Query: 398  410
           
Sbjct: 556  314

[Close]

BLAST of CU120115 vs. TrEMBL
Match: B9SDU2_RICCO (tRNA pseudouridine synthase OS=Ricinus communis GN=RCOM_1712560 PE=3 SV=1)

HSP 1 Score: 124.4 bits (311), Expect = 1.1e-25
Identity = 63/124 (50.81%), Postives = 81/124 (65.32%), Query Frame = -1


Query: 38  RQVCSFYTWRTDVKPQDIEDSINSAAPGKLRISSVSQVSRIFHPNFSAKWRRYLYILPLN 97
           RQVCSFYTWR DV+P +IED+INS+APGK+R+ SVS+VSR+FHPNFSAKWRRYLYI P+N
Sbjct: 190 RQVCSFYTWRKDVRPHEIEDAINSSAPGKIRVISVSEVSRVFHPNFSAKWRRYLYIFPIN 249

Query: 98  DGENMEPDSTTWXXXXXXXXXXFVKIEASNGEKCVDGIHDNLVLSPNDEELVSADKPQTF 157
           DGE+ E    +           + +      E   +   + L+ S  D EL  A KP+ F
Sbjct: 250 DGEDSEQSFDSEDLENLRSYEKYDEQRNGCAELTSEEHVEELITSDKD-ELEGAKKPRIF 309

Query: 158 SITK 217
           SI +
Sbjct: 310 SICR 312

Query: 218  277
           
Sbjct: 370  312

Query: 278  337
           
Sbjct: 430  312

Query: 338  397
           
Sbjct: 490  312

Query: 398  410
           
Sbjct: 550  312

[Close]

BLAST of CU120115 vs. NCBI nr
Match: gi|449439176|ref|XP_004137363.1| (PREDICTED: uncharacterized protein LOC101205562 [Cucumis sativus])

HSP 1 Score: 234.2 bits (596), Expect = 1.4e-58
Identity = 111/124 (89.52%), Postives = 113/124 (91.13%), Query Frame = -1


Query: 38  RQVCSFYTWRTDVKPQDIEDSINSAAPGKLRISSVSQVSRIFHPNFSAKWRRYLYILPLN 97
           RQVCSFYTWRTDVKPQDIEDSINSAAPGKLR SSVSQVSRIFHPNFSAKWRRYLYILPLN
Sbjct: 188 RQVCSFYTWRTDVKPQDIEDSINSAAPGKLRTSSVSQVSRIFHPNFSAKWRRYLYILPLN 247

Query: 98  DGENMEPDSTTWXXXXXXXXXXFVKIEASNGEKCVDGIHDNLVLSPNDEELVSADKPQTF 157
           DGENMEPDSTTW          FVKIEASNGEKCVDGIHDNLVLSPND+ELVSADKPQTF
Sbjct: 248 DGENMEPDSTTWENVENREEENFVKIEASNGEKCVDGIHDNLVLSPNDQELVSADKPQTF 307

Query: 158 SITK 217
           SIT+
Sbjct: 308 SITR 311

Query: 218  277
           
Sbjct: 368  311

Query: 278  337
           
Sbjct: 428  311

Query: 338  397
           
Sbjct: 488  311

Query: 398  410
           
Sbjct: 548  311

[Close]

BLAST of CU120115 vs. NCBI nr
Match: gi|659107096|ref|XP_008453520.1| (PREDICTED: uncharacterized protein LOC103494203 [Cucumis melo])

HSP 1 Score: 219.9 bits (559), Expect = 2.7e-54
Identity = 105/124 (84.68%), Postives = 107/124 (86.29%), Query Frame = -1


Query: 38  RQVCSFYTWRTDVKPQDIEDSINSAAPGKLRISSVSQVSRIFHPNFSAKWRRYLYILPLN 97
           RQVCSFYTWRTDVKPQDIEDSINSAAPGKLR  SVSQVSRIFHPNFSAKWRRYLYILPLN
Sbjct: 188 RQVCSFYTWRTDVKPQDIEDSINSAAPGKLRTLSVSQVSRIFHPNFSAKWRRYLYILPLN 247

Query: 98  DGENMEPDSTTWXXXXXXXXXXFVKIEASNGEKCVDGIHDNLVLSPNDEELVSADKPQTF 157
           DGENMEPDS  W           VKIEA NGEKCVDGIHDNLVLSPND+ELVSADKPQTF
Sbjct: 248 DGENMEPDSAIWEDVENREEENCVKIEALNGEKCVDGIHDNLVLSPNDDELVSADKPQTF 307

Query: 158 SITK 217
           SI +
Sbjct: 308 SIRR 311

Query: 218  277
           
Sbjct: 368  311

Query: 278  337
           
Sbjct: 428  311

Query: 338  397
           
Sbjct: 488  311

Query: 398  410
           
Sbjct: 548  311

[Close]

BLAST of CU120115 vs. NCBI nr
Match: gi|566215861|ref|XP_006372225.1| (tRNA pseudouridine synthase family protein [Populus trichocarpa])

HSP 1 Score: 130.6 bits (327), Expect = 2.1e-27
Identity = 63/124 (50.81%), Postives = 81/124 (65.32%), Query Frame = -1


Query: 38  RQVCSFYTWRTDVKPQDIEDSINSAAPGKLRISSVSQVSRIFHPNFSAKWRRYLYILPLN 97
           +QVCSFYTWR DVKP +IED+IN  APGK+R+ S+S+VSR FHPNFSAKWRRYLYI PLN
Sbjct: 204 QQVCSFYTWRKDVKPHEIEDAINDVAPGKVRVESISEVSRAFHPNFSAKWRRYLYIFPLN 263

Query: 98  DGENMEPDSTTWXXXXXXXXXXFVKIEASNGEKCVDGIHDNLVLSPNDEELVSADKPQTF 157
           DGEN E                  K     GE   +   +N ++S +++EL  A KP++F
Sbjct: 264 DGENREEIEGEGGIENFSSHENCEKQRNECGELASEENVENSIIS-DEDELQGAKKPRSF 323

Query: 158 SITK 217
           S+ +
Sbjct: 324 SVCR 326

Query: 218  277
           
Sbjct: 384  326

Query: 278  337
           
Sbjct: 444  326

Query: 338  397
           
Sbjct: 504  326

Query: 398  410
           
Sbjct: 564  326

[Close]

BLAST of CU120115 vs. NCBI nr
Match: gi|566215859|ref|XP_006372224.1| (hypothetical protein POPTR_0018s14400g [Populus trichocarpa])

HSP 1 Score: 130.6 bits (327), Expect = 2.1e-27
Identity = 63/124 (50.81%), Postives = 81/124 (65.32%), Query Frame = -1


Query: 38  RQVCSFYTWRTDVKPQDIEDSINSAAPGKLRISSVSQVSRIFHPNFSAKWRRYLYILPLN 97
           +QVCSFYTWR DVKP +IED+IN  APGK+R+ S+S+VSR FHPNFSAKWRRYLYI PLN
Sbjct: 195 QQVCSFYTWRKDVKPHEIEDAINDVAPGKVRVESISEVSRAFHPNFSAKWRRYLYIFPLN 254

Query: 98  DGENMEPDSTTWXXXXXXXXXXFVKIEASNGEKCVDGIHDNLVLSPNDEELVSADKPQTF 157
           DGEN E                  K     GE   +   +N ++S +++EL  A KP++F
Sbjct: 255 DGENREEIEGEGGIENFSSHENCEKQRNECGELASEENVENSIIS-DEDELQGAKKPRSF 314

Query: 158 SITK 217
           S+ +
Sbjct: 315 SVCR 317

Query: 218  277
           
Sbjct: 375  317

Query: 278  337
           
Sbjct: 435  317

Query: 338  397
           
Sbjct: 495  317

Query: 398  410
           
Sbjct: 555  317

[Close]

BLAST of CU120115 vs. NCBI nr
Match: gi|743805337|ref|XP_011017609.1| (PREDICTED: uncharacterized protein LOC105120901 isoform X3 [Populus euphratica])

HSP 1 Score: 129.0 bits (323), Expect = 6.2e-27
Identity = 62/124 (50.00%), Postives = 80/124 (64.52%), Query Frame = -1


Query: 38  RQVCSFYTWRTDVKPQDIEDSINSAAPGKLRISSVSQVSRIFHPNFSAKWRRYLYILPLN 97
           +QVCSFYTWR DVKP +IED+IN  APGK+R+ S+S+VSR FHPNFSAKWRRYLYI PLN
Sbjct: 195 QQVCSFYTWRKDVKPHEIEDAINDVAPGKIRVESISEVSRAFHPNFSAKWRRYLYIFPLN 254

Query: 98  DGENMEPDSTTWXXXXXXXXXXFVKIEASNGEKCVDGIHDNLVLSPNDEELVSADKPQTF 157
           DGEN E                        GE   +   +N ++S +++EL  A KP++F
Sbjct: 255 DGENREEIEGEGDIENFTSHENCENQRNECGELASEENIENSIIS-DEDELEGAKKPRSF 314

Query: 158 SITK 217
           S+ +
Sbjct: 315 SVCR 317

Query: 218  277
           
Sbjct: 375  317

Query: 278  337
           
Sbjct: 435  317

Query: 338  397
           
Sbjct: 495  317

Query: 398  410
           
Sbjct: 555  317

The following BLAST results are available for this feature:

BLAST of CU120115 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LV59_CUCSA	1.8e-57	89.52	tRNA pseudouridine synthase OS=Cucumis sativus GN=Csa_1G025830 PE=3 SV=1	[more]
U5FKW5_POPTR	2.8e-26	50.81	tRNA pseudouridine synthase OS=Populus trichocarpa GN=POPTR_0018s14400g PE=3 SV=...	[more]
U5FJE5_POPTR	2.8e-26	50.81	tRNA pseudouridine synthase OS=Populus trichocarpa GN=POPTR_0018s14400g PE=3 SV=...	[more]
V4UJT6_9ROSI	8.1e-26	55.20	tRNA pseudouridine synthase OS=Citrus clementina GN=CICLE_v10008288mg PE=3 SV=1	[more]
B9SDU2_RICCO	1.1e-25	50.81	tRNA pseudouridine synthase OS=Ricinus communis GN=RCOM_1712560 PE=3 SV=1	[more]

BLAST of CU120115 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449439176\|ref\|XP_004137363.1\|	1.4e-58	89.52	PREDICTED: uncharacterized protein LOC101205562 [Cucumis sativus]	[more]
gi\|659107096\|ref\|XP_008453520.1\|	2.7e-54	84.68	PREDICTED: uncharacterized protein LOC103494203 [Cucumis melo]	[more]
gi\|566215861\|ref\|XP_006372225.1\|	2.1e-27	50.81	tRNA pseudouridine synthase family protein [Populus trichocarpa]	[more]
gi\|566215859\|ref\|XP_006372224.1\|	2.1e-27	50.81	hypothetical protein POPTR_0018s14400g [Populus trichocarpa]	[more]
gi\|743805337\|ref\|XP_011017609.1\|	6.2e-27	50.00	PREDICTED: uncharacterized protein LOC105120901 isoform X3 [Populus euphratica]	[more]

Annotated Terms

The following terms have been associated with this transcribed_cluster:

Vocabulary: **INTERPRO**
Term	Definition
IPR001406	PsdUridine_synth_TruA
IPR020103	PsdUridine_synth_cat_dom_sf

Vocabulary: **Biological Process**
Term	Definition
GO:0001522	pseudouridine synthesis
GO:0009451	RNA modification

Vocabulary: **Molecular Function**
Term	Definition
GO:0003723	RNA binding
GO:0009982	pseudouridine synthase activity

GO Annotation

GO Assignments

This transcribed_cluster is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0001522	pseudouridine synthesis
biological_process	GO:0009451	RNA modification
molecular_function	GO:0009982	pseudouridine synthase activity
molecular_function	GO:0003723	RNA binding

Relationships

This transcribed_cluster is associated with the following gene feature(s):


Feature Name	Unique Name	Type
Csa1G025830	Csa1G025830	gene

The following EST feature(s) are a part of this transcribed_cluster:


Feature Name	Unique Name	Type
FKNP3UI02ONW1A	FKNP3UI02ONW1A	EST
G0037770	G0037770	EST

InterPro

Analysis Name: InterPro Annotations of cucumber unigene v3
Date Performed: 2016-11-16


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001406	Pseudouridine synthase I, TruA	PANTHER	PTHR11142	PSEUDOURIDYLATE SYNTHASE	coord: 116..136 score: 1.6E-45coord: 1..78 score: 1.6
IPR020103	Pseudouridine synthase, catalytic domain	unknown	SSF55120	Pseudouridine synthase	coord: 2..73 score: 4.1
None	No IPR available	PANTHER	PTHR11142:SF10	TRNA PSEUDOURIDINE SYNTHASE	coord: 116..136 score: 1.6E-45coord: 1..78 score: 1.6