CU102986 (transcribed_cluster) Cucumber (Chinese Long) v2 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CU102986
Type	transcribed_cluster
Organism	Cucumis. sativus (Cucumber (Chinese Long) v2)
Description	Diacylglycerol kinase family protein
Location	Chr3 : 14246559 .. 14247859 (+)
Sequence length	393

Genome Viewer

Sequences

The following sequences are available for this feature:

transcribed_cluster sequence

TTAAAACCCAAATATTCATTCGAGGTTGATGACGGTGAATGGGAGCTGTATCCACAAGTAACTGCTCTATGCATTGGAAATGCTAAATACTTTGGCGGTGGTATGAAAATTGTGCCAAATGTCGATCCTTCCAACAGGAGTCTAGAGGTAGTAATTCTTCAGGATTTCAAGTGGTATGACTTTATTCTGAATTTACACAAAATATACAATGGGACATATTTGACGGTGAAAAACGTAACATCAAGAAGCGTTCGTTCGATCGAAGTGGAAGAAGTTTCTTGCAGCGGCAGCATATATGTTCAATCTGATGGAGAACACTTGGGGTTTCTTCCAAGGAAATTCCATATTCTACCAGCTGCCATTGAAATGATATGCTGAACATGAGCTCCCTTT

Library


Library category	ESTs
fruit (8DPP) library	1
gynoecious flower library	1
hermaphrodite flower library	1
Cucumber male flower buds	1

Homology

[Close]

BLAST of CU102986 vs. Swiss-Prot
Match: LCKB2_ARATH (Sphingoid long-chain bases kinase 2, mitochondrial OS=Arabidopsis thaliana GN=LCKB2 PE=2 SV=1)

HSP 1 Score: 196.1 bits (497), Expect = 2.5e-49
Identity = 89/116 (76.72%), Postives = 103/116 (88.79%), Query Frame = 1


Query: 25  VDDGEWELYPQVTALCIGNAKYFGGGMKIVPNVDPSNRSLEVVILQDFKWYDFILNLHKI 84
           V+ GEWE+YPQVTALC+GNAKYFGGGMKI PN  P N +LEVV+LQ+FKWYDF+L LHK+
Sbjct: 248 VNGGEWEIYPQVTALCVGNAKYFGGGMKITPNAIPGNGNLEVVVLQNFKWYDFVLKLHKL 307

Query: 85  YNGTYLTVKNVTSRSVRSIEVEEVSCSGSIYVQSDGEHLGFLPRKFHILPAAIEMI 144
           YNGT+L+V NVTSRSV+SIEVEE++ SGSIYVQSDGEHLGFLPRKF +LP AI++I
Sbjct: 308 YNGTHLSVNNVTSRSVQSIEVEEITDSGSIYVQSDGEHLGFLPRKFQVLPGAIDII 363

Query: 145  204
           
Sbjct: 368  363

Query: 205  264
           
Sbjct: 428  363

Query: 265  324
           
Sbjct: 488  363

Query: 325  373
           
Sbjct: 548  363

[Close]

BLAST of CU102986 vs. TrEMBL
Match: A0A0A0K3Y3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G280850 PE=4 SV=1)

HSP 1 Score: 243.0 bits (619), Expect = 2.0e-61
Identity = 116/121 (95.87%), Postives = 118/121 (97.52%), Query Frame = 1


Query: 13  YSFEVDDGEWELYPQVTALCIGNAKYFGGGMKIVPNVDPSNRSLEVVILQDFKWYDFILN 72
           +  +VDDGEWELYPQVTALCIGNAKYFGGGMKIVPN DPSNRSLEVVILQDFKWYDFILN
Sbjct: 121 FKTKVDDGEWELYPQVTALCIGNAKYFGGGMKIVPNADPSNRSLEVVILQDFKWYDFILN 180

Query: 73  LHKIYNGTYLTVKNVTSRSVRSIEVEEVSCSGSIYVQSDGEHLGFLPRKFHILPAAIEMI 132
           LHKIYNGTYLTVKNVTSRSVRSIEVEEVSCSGSIYVQSDGEHLGFLPRKFHILPAAIEMI
Sbjct: 181 LHKIYNGTYLTVKNVTSRSVRSIEVEEVSCSGSIYVQSDGEHLGFLPRKFHILPAAIEMI 240

Query: 133 C 192
           C
Sbjct: 241 C 241

Query: 193  252
           
Sbjct: 301  241

Query: 253  312
           
Sbjct: 361  241

Query: 313  372
           
Sbjct: 421  241

Query: 373  376
           
Sbjct: 481  241

[Close]

BLAST of CU102986 vs. TrEMBL
Match: A0A0A0KD28_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G006770 PE=4 SV=1)

HSP 1 Score: 243.0 bits (619), Expect = 2.0e-61
Identity = 116/121 (95.87%), Postives = 119/121 (98.35%), Query Frame = 1


Query: 13  YSFEVDDGEWELYPQVTALCIGNAKYFGGGMKIVPNVDPSNRSLEVVILQDFKWYDFILN 72
           +  +VDDGEWELYPQVTALCIGNAKYFGGGMKIVPNVDPSNRSLEVVILQDFKWYDFILN
Sbjct: 85  FRIKVDDGEWELYPQVTALCIGNAKYFGGGMKIVPNVDPSNRSLEVVILQDFKWYDFILN 144

Query: 73  LHKIYNGTYLTVKNVTSRSVRSIEVEEVSCSGSIYVQSDGEHLGFLPRKFHILPAAIEMI 132
           LHKIYNGT+LTVKNVTSRSVRSIEVEEVSCSGSIYVQSDGEHLGFLPRKFHILPAAIEMI
Sbjct: 145 LHKIYNGTHLTVKNVTSRSVRSIEVEEVSCSGSIYVQSDGEHLGFLPRKFHILPAAIEMI 204

Query: 133 C 192
           C
Sbjct: 205 C 205

Query: 193  252
           
Sbjct: 265  205

Query: 253  312
           
Sbjct: 325  205

Query: 313  372
           
Sbjct: 385  205

Query: 373  376
           
Sbjct: 445  205

[Close]

BLAST of CU102986 vs. TrEMBL
Match: A0A0A0L9B2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G211980 PE=4 SV=1)

HSP 1 Score: 237.7 bits (605), Expect = 8.3e-60
Identity = 115/121 (95.04%), Postives = 115/121 (95.04%), Query Frame = 1


Query: 13  YSFEVDDGEWELYPQVTALCIGNAKYFGGGMKIVPNVDPSNRSLEVVILQDFKWYDFILN 72
           Y   VD  EWELYPQVTALCIGNAKYFGGGMKIVPN DPSNRSLEVVILQDFKWYDFILN
Sbjct: 180 YFANVDYAEWELYPQVTALCIGNAKYFGGGMKIVPNADPSNRSLEVVILQDFKWYDFILN 239

Query: 73  LHKIYNGTYLTVKNVTSRSVRSIEVEEVSCSGSIYVQSDGEHLGFLPRKFHILPAAIEMI 132
           LHKIYNGTYLTVKNVTSRSVRSIEVEEVSCSGSIYVQSDGEHLGFLPRKFHILPAAIEMI
Sbjct: 240 LHKIYNGTYLTVKNVTSRSVRSIEVEEVSCSGSIYVQSDGEHLGFLPRKFHILPAAIEMI 299

Query: 133 C 192
           C
Sbjct: 300 C 300

Query: 193  252
           
Sbjct: 360  300

Query: 253  312
           
Sbjct: 420  300

Query: 313  372
           
Sbjct: 480  300

Query: 373  376
           
Sbjct: 540  300

[Close]

BLAST of CU102986 vs. TrEMBL
Match: D7UCH8_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_15s0046g01770 PE=4 SV=1)

HSP 1 Score: 204.5 bits (519), Expect = 7.8e-50
Identity = 96/117 (82.05%), Postives = 108/117 (92.31%), Query Frame = 1


Query: 22  EVDDGEWELYPQVTALCIGNAKYFGGGMKIVPNVDPSNRSLEVVILQDFKWYDFILNLHK 81
           ++DDGEWE+YPQVTALCIGNAK+FGGGMKI PN DP + S EVVILQDFKWYDFILNLHK
Sbjct: 228 KIDDGEWEIYPQVTALCIGNAKFFGGGMKITPNADPRSGSFEVVILQDFKWYDFILNLHK 287

Query: 82  IYNGTYLTVKNVTSRSVRSIEVEEVSCSGSIYVQSDGEHLGFLPRKFHILPAAIEMI 141
           +Y GT+L+VKNV+SRSVR IEVEE++ SGSI+VQSDGEHLGFLPRKF ILPAAIEMI
Sbjct: 288 LYKGTHLSVKNVSSRSVRCIEVEEIAGSGSIHVQSDGEHLGFLPRKFCILPAAIEMI 344

Query: 142  201
           
Sbjct: 348  344

Query: 202  261
           
Sbjct: 408  344

Query: 262  321
           
Sbjct: 468  344

Query: 322  373
           
Sbjct: 528  344

[Close]

BLAST of CU102986 vs. TrEMBL
Match: A0A059CJK2_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_D02318 PE=4 SV=1)

HSP 1 Score: 203.8 bits (517), Expect = 1.3e-49
Identity = 93/121 (76.86%), Postives = 109/121 (90.08%), Query Frame = 1


Query: 13  YSFEVDDGEWELYPQVTALCIGNAKYFGGGMKIVPNVDPSNRSLEVVILQDFKWYDFILN 72
           +  +V+DGEWE YPQVTALCIGNAK+FGGGMKI PN DPS+ + EVVILQDF+WYDFIL 
Sbjct: 249 FRIKVNDGEWETYPQVTALCIGNAKFFGGGMKITPNADPSSGNFEVVILQDFRWYDFILK 308

Query: 73  LHKIYNGTYLTVKNVTSRSVRSIEVEEVSCSGSIYVQSDGEHLGFLPRKFHILPAAIEMI 132
           LHK+YNGT+L+V NV+SRSV SIEVEE+S +GSI+VQSDGEHLGFLPRKF +LP+AIEMI
Sbjct: 309 LHKLYNGTHLSVNNVSSRSVHSIEVEEISGTGSIFVQSDGEHLGFLPRKFRVLPSAIEMI 368

Query: 133 C 192
           C
Sbjct: 369 C 369

Query: 193  252
           
Sbjct: 429  369

Query: 253  312
           
Sbjct: 489  369

Query: 313  372
           
Sbjct: 549  369

Query: 373  376
           
Sbjct: 609  369

[Close]

BLAST of CU102986 vs. NCBI nr
Match: gi|778721950|ref|XP_011658380.1| (PREDICTED: sphingoid long-chain bases kinase 2, mitochondrial [Cucumis sativus])

HSP 1 Score: 249.2 bits (635), Expect = 3.9e-63
Identity = 116/121 (95.87%), Postives = 119/121 (98.35%), Query Frame = 1


Query: 13  YSFEVDDGEWELYPQVTALCIGNAKYFGGGMKIVPNVDPSNRSLEVVILQDFKWYDFILN 72
           +  +VDDGEWELYPQVTALCIGNAKYFGGGMKIVPNVDPSNRSLEVVILQDFKWYDFILN
Sbjct: 256 FRIKVDDGEWELYPQVTALCIGNAKYFGGGMKIVPNVDPSNRSLEVVILQDFKWYDFILN 315

Query: 73  LHKIYNGTYLTVKNVTSRSVRSIEVEEVSCSGSIYVQSDGEHLGFLPRKFHILPAAIEMI 132
           LHKIYNGT+LTVKNVTSRSVRSIEVEEVSCSGSIYVQSDGEHLGFLPRKFHILPAAIEMI
Sbjct: 316 LHKIYNGTHLTVKNVTSRSVRSIEVEEVSCSGSIYVQSDGEHLGFLPRKFHILPAAIEMI 375

Query: 133 C 192
           C
Sbjct: 376 C 376

Query: 193  252
           
Sbjct: 436  376

Query: 253  312
           
Sbjct: 496  376

Query: 313  372
           
Sbjct: 556  376

Query: 373  376
           
Sbjct: 616  376

[Close]

BLAST of CU102986 vs. NCBI nr
Match: gi|700189172|gb|KGN44405.1| (hypothetical protein Csa_7G280850 [Cucumis sativus])

HSP 1 Score: 249.2 bits (635), Expect = 3.9e-63
Identity = 116/121 (95.87%), Postives = 118/121 (97.52%), Query Frame = 1


Query: 13  YSFEVDDGEWELYPQVTALCIGNAKYFGGGMKIVPNVDPSNRSLEVVILQDFKWYDFILN 72
           +  +VDDGEWELYPQVTALCIGNAKYFGGGMKIVPN DPSNRSLEVVILQDFKWYDFILN
Sbjct: 121 FKTKVDDGEWELYPQVTALCIGNAKYFGGGMKIVPNADPSNRSLEVVILQDFKWYDFILN 180

Query: 73  LHKIYNGTYLTVKNVTSRSVRSIEVEEVSCSGSIYVQSDGEHLGFLPRKFHILPAAIEMI 132
           LHKIYNGTYLTVKNVTSRSVRSIEVEEVSCSGSIYVQSDGEHLGFLPRKFHILPAAIEMI
Sbjct: 181 LHKIYNGTYLTVKNVTSRSVRSIEVEEVSCSGSIYVQSDGEHLGFLPRKFHILPAAIEMI 240

Query: 133 C 192
           C
Sbjct: 241 C 241

Query: 193  252
           
Sbjct: 301  241

Query: 253  312
           
Sbjct: 361  241

Query: 313  372
           
Sbjct: 421  241

Query: 373  376
           
Sbjct: 481  241

[Close]

BLAST of CU102986 vs. NCBI nr
Match: gi|778730292|ref|XP_011659752.1| (PREDICTED: sphingoid long-chain bases kinase 2, mitochondrial-like [Cucumis sativus])

HSP 1 Score: 249.2 bits (635), Expect = 3.9e-63
Identity = 116/121 (95.87%), Postives = 118/121 (97.52%), Query Frame = 1


Query: 13  YSFEVDDGEWELYPQVTALCIGNAKYFGGGMKIVPNVDPSNRSLEVVILQDFKWYDFILN 72
           +  +VDDGEWELYPQVTALCIGNAKYFGGGMKIVPN DPSNRSLEVVILQDFKWYDFILN
Sbjct: 122 FKTKVDDGEWELYPQVTALCIGNAKYFGGGMKIVPNADPSNRSLEVVILQDFKWYDFILN 181

Query: 73  LHKIYNGTYLTVKNVTSRSVRSIEVEEVSCSGSIYVQSDGEHLGFLPRKFHILPAAIEMI 132
           LHKIYNGTYLTVKNVTSRSVRSIEVEEVSCSGSIYVQSDGEHLGFLPRKFHILPAAIEMI
Sbjct: 182 LHKIYNGTYLTVKNVTSRSVRSIEVEEVSCSGSIYVQSDGEHLGFLPRKFHILPAAIEMI 241

Query: 133 C 192
           C
Sbjct: 242 C 242

Query: 193  252
           
Sbjct: 302  242

Query: 253  312
           
Sbjct: 362  242

Query: 313  372
           
Sbjct: 422  242

Query: 373  376
           
Sbjct: 482  242

[Close]

BLAST of CU102986 vs. NCBI nr
Match: gi|700190487|gb|KGN45691.1| (hypothetical protein Csa_6G006770 [Cucumis sativus])

HSP 1 Score: 249.2 bits (635), Expect = 3.9e-63
Identity = 116/121 (95.87%), Postives = 119/121 (98.35%), Query Frame = 1


Query: 13  YSFEVDDGEWELYPQVTALCIGNAKYFGGGMKIVPNVDPSNRSLEVVILQDFKWYDFILN 72
           +  +VDDGEWELYPQVTALCIGNAKYFGGGMKIVPNVDPSNRSLEVVILQDFKWYDFILN
Sbjct: 85  FRIKVDDGEWELYPQVTALCIGNAKYFGGGMKIVPNVDPSNRSLEVVILQDFKWYDFILN 144

Query: 73  LHKIYNGTYLTVKNVTSRSVRSIEVEEVSCSGSIYVQSDGEHLGFLPRKFHILPAAIEMI 132
           LHKIYNGT+LTVKNVTSRSVRSIEVEEVSCSGSIYVQSDGEHLGFLPRKFHILPAAIEMI
Sbjct: 145 LHKIYNGTHLTVKNVTSRSVRSIEVEEVSCSGSIYVQSDGEHLGFLPRKFHILPAAIEMI 204

Query: 133 C 192
           C
Sbjct: 205 C 205

Query: 193  252
           
Sbjct: 265  205

Query: 253  312
           
Sbjct: 325  205

Query: 313  372
           
Sbjct: 385  205

Query: 373  376
           
Sbjct: 445  205

[Close]

BLAST of CU102986 vs. NCBI nr
Match: gi|659116624|ref|XP_008458170.1| (PREDICTED: sphingoid long-chain bases kinase 2, mitochondrial isoform X1 [Cucumis melo])

HSP 1 Score: 243.8 bits (621), Expect = 1.7e-61
Identity = 112/121 (92.56%), Postives = 117/121 (96.69%), Query Frame = 1


Query: 13  YSFEVDDGEWELYPQVTALCIGNAKYFGGGMKIVPNVDPSNRSLEVVILQDFKWYDFILN 72
           +  +VDDGEWELYPQVTALCIGNAKYFGGGMKIVPN DPSN SLEVVILQDFKWYDFILN
Sbjct: 253 FKIKVDDGEWELYPQVTALCIGNAKYFGGGMKIVPNADPSNGSLEVVILQDFKWYDFILN 312

Query: 73  LHKIYNGTYLTVKNVTSRSVRSIEVEEVSCSGSIYVQSDGEHLGFLPRKFHILPAAIEMI 132
           LHKIYNGT+LTVKNVTS+S+RSIEVEEVSCSGSIYVQSDGEHLGFLPRKFHILPAAIEMI
Sbjct: 313 LHKIYNGTHLTVKNVTSKSIRSIEVEEVSCSGSIYVQSDGEHLGFLPRKFHILPAAIEMI 372

Query: 133 C 192
           C
Sbjct: 373 C 373

Query: 193  252
           
Sbjct: 433  373

Query: 253  312
           
Sbjct: 493  373

Query: 313  372
           
Sbjct: 553  373

Query: 373  376
           
Sbjct: 613  373

The following BLAST results are available for this feature:

BLAST of CU102986 vs. Swiss-Prot


Match Name	E-value	Identity	Description
LCKB2_ARATH	2.5e-49	76.72	Sphingoid long-chain bases kinase 2, mitochondrial OS=Arabidopsis thaliana GN=LC...	[more]

BLAST of CU102986 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K3Y3_CUCSA	2.0e-61	95.87	Uncharacterized protein OS=Cucumis sativus GN=Csa_7G280850 PE=4 SV=1	[more]
A0A0A0KD28_CUCSA	2.0e-61	95.87	Uncharacterized protein OS=Cucumis sativus GN=Csa_6G006770 PE=4 SV=1	[more]
A0A0A0L9B2_CUCSA	8.3e-60	95.04	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G211980 PE=4 SV=1	[more]
D7UCH8_VITVI	7.8e-50	82.05	Putative uncharacterized protein OS=Vitis vinifera GN=VIT_15s0046g01770 PE=4 SV=...	[more]
A0A059CJK2_EUCGR	1.3e-49	76.86	Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_D02318 PE=4 SV=1	[more]

BLAST of CU102986 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|778721950\|ref\|XP_011658380.1\|	3.9e-63	95.87	PREDICTED: sphingoid long-chain bases kinase 2, mitochondrial [Cucumis sativus]	[more]
gi\|700189172\|gb\|KGN44405.1\|	3.9e-63	95.87	hypothetical protein Csa_7G280850 [Cucumis sativus]	[more]
gi\|778730292\|ref\|XP_011659752.1\|	3.9e-63	95.87	PREDICTED: sphingoid long-chain bases kinase 2, mitochondrial-like [Cucumis sati...	[more]
gi\|700190487\|gb\|KGN45691.1\|	3.9e-63	95.87	hypothetical protein Csa_6G006770 [Cucumis sativus]	[more]
gi\|659116624\|ref\|XP_008458170.1\|	1.7e-61	92.56	PREDICTED: sphingoid long-chain bases kinase 2, mitochondrial isoform X1 [Cucumi...	[more]

Annotated Terms

The following terms have been associated with this transcribed_cluster:

Vocabulary: **INTERPRO**
Term	Definition
IPR016064	NAD/diacylglycerol_kinase_sf

Relationships

This transcribed_cluster is associated with the following gene feature(s):


Feature Name	Unique Name	Type
Csa3G211980	Csa3G211980	gene

The following EST feature(s) are a part of this transcribed_cluster:


Feature Name	Unique Name	Type
FKNP3UI02K70GX	FKNP3UI02K70GX	EST
G0188536	G0188536	EST
H0120034	H0120034	EST
csa02-3ms2-b11	csa02-3ms2-b11	EST

InterPro

Analysis Name: InterPro Annotations of cucumber unigene v3
Date Performed: 2016-11-16


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR016064	NAD kinase/diacylglycerol kinase-like domain	unknown	SSF111331	NAD kinase/diacylglycerol kinase-like	coord: 4..125 score: 1.02
None	No IPR available	PANTHER	PTHR12358	SPHINGOSINE KINASE	coord: 8..125 score: 2.4
None	No IPR available	PANTHER	PTHR12358:SF43	SPHINGOID LONG-CHAIN BASES KINASE 2, MITOCHONDRIAL	coord: 8..125 score: 2.4