CU100317 (transcribed_cluster) Cucumber (Chinese Long) v2 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CU100317
Type	transcribed_cluster
Organism	Cucumis. sativus (Cucumber (Chinese Long) v2)
Description	Histone H2B
Location	Chr1 : 9434122 .. 9434387 (-)
Sequence length	271

Genome Viewer

Sequences

The following sequences are available for this feature:

transcribed_cluster sequence

GCTCGCTACAACAAGAAGCCGACTATTCTACCTTCTTCGGGTAGATCCAAACGGCGGTGCGCCTTGTTCTTCCTGGTGAGCTGGCTAAACACGCTGTCTCTGAGGGTACCAAGGCTGTTACCAAGTTCACTAGCTCTTAGATTCATAGATTTAGGGTTTTCTCGTGTAAAAAAGCAGGATCACTTGTTCTGGATCGTGGATTTTCTTTTCCTTTTGTTGCATATTTTTGGAATTGAATGAATGAAAATCGGTTTACATAACCCGAATTCTT

Library


Library category	ESTs
hermaphrodite flower library	2

Homology

[Close]

BLAST of CU100317 vs. Swiss-Prot
Match: H2B3_ARATH (Histone H2B.3 OS=Arabidopsis thaliana GN=At2g28720 PE=1 SV=3)

HSP 1 Score: 61.6 bits (148), Expect = 5.0e-09
Identity = 31/31 (100.00%), Postives = 29/31 (93.55%), Query Frame = 3


Query: 45  IQTAVRLVLPGELAKHAVSEGTKAVTKFTSS 104
           IQTAVRLVLPGELAKHAVSEGTKAVTKFTSS
Sbjct: 121 IQTAVRLVLPGELAKHAVSEGTKAVTKFTSS 151

Query: 105  138
           
Sbjct: 181  151

[Close]

BLAST of CU100317 vs. Swiss-Prot
Match: H2B6_ORYSJ (Histone H2B.6 OS=Oryza sativa subsp. japonica GN=H2B.6 PE=3 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 5.0e-09
Identity = 31/31 (100.00%), Postives = 29/31 (93.55%), Query Frame = 3


Query: 45  IQTAVRLVLPGELAKHAVSEGTKAVTKFTSS 104
           IQTAVRLVLPGELAKHAVSEGTKAVTKFTSS
Sbjct: 123 IQTAVRLVLPGELAKHAVSEGTKAVTKFTSS 153

Query: 105  138
           
Sbjct: 183  153

[Close]

BLAST of CU100317 vs. Swiss-Prot
Match: H2B_GOSHI (Histone H2B OS=Gossypium hirsutum GN=HIS2B PE=2 SV=3)

HSP 1 Score: 61.6 bits (148), Expect = 5.0e-09
Identity = 31/31 (100.00%), Postives = 29/31 (93.55%), Query Frame = 3


Query: 45  IQTAVRLVLPGELAKHAVSEGTKAVTKFTSS 104
           IQTAVRLVLPGELAKHAVSEGTKAVTKFTSS
Sbjct: 117 IQTAVRLVLPGELAKHAVSEGTKAVTKFTSS 147

Query: 105  138
           
Sbjct: 177  147

[Close]

BLAST of CU100317 vs. Swiss-Prot
Match: H2B11_ARATH (Histone H2B.11 OS=Arabidopsis thaliana GN=At5g59910 PE=1 SV=5)

HSP 1 Score: 61.6 bits (148), Expect = 5.0e-09
Identity = 31/31 (100.00%), Postives = 29/31 (93.55%), Query Frame = 3


Query: 45  IQTAVRLVLPGELAKHAVSEGTKAVTKFTSS 104
           IQTAVRLVLPGELAKHAVSEGTKAVTKFTSS
Sbjct: 120 IQTAVRLVLPGELAKHAVSEGTKAVTKFTSS 150

Query: 105  138
           
Sbjct: 180  150

[Close]

BLAST of CU100317 vs. Swiss-Prot
Match: H2B_TOBAC (Histone H2B OS=Nicotiana tabacum GN=HIS2B PE=2 SV=3)

HSP 1 Score: 61.6 bits (148), Expect = 5.0e-09
Identity = 31/31 (100.00%), Postives = 29/31 (93.55%), Query Frame = 3


Query: 45  IQTAVRLVLPGELAKHAVSEGTKAVTKFTSS 104
           IQTAVRLVLPGELAKHAVSEGTKAVTKFTSS
Sbjct: 116 IQTAVRLVLPGELAKHAVSEGTKAVTKFTSS 146

Query: 105  138
           
Sbjct: 176  146

[Close]

BLAST of CU100317 vs. TrEMBL
Match: I3JKL4_ORENI (Histone H2B OS=Oreochromis niloticus GN=HIST1H2BA PE=3 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 1.9e-07
Identity = 31/39 (79.49%), Postives = 35/39 (89.74%), Query Frame = 3


Query: 45  IQTAVRLVLPGELAKHAVSEGTKAVTKFTSS*IHRFRVF 104
           IQTAVRL+LPGELAKHAVSEGTKAVTK+TSS +H   +F
Sbjct: 93  IQTAVRLLLPGELAKHAVSEGTKAVTKYTSSKVHVLTLF 131

Query: 105  162
           
Sbjct: 153  131

[Close]

BLAST of CU100317 vs. TrEMBL
Match: A0A0N4VGK6_ENTVE (Histone H4 OS=Enterobius vermicularis PE=3 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 2.5e-07
Identity = 32/47 (68.09%), Postives = 38/47 (80.85%), Query Frame = 3


Query: 45  IQTAVRLVLPGELAKHAVSEGTKAVTKFTSS*IHRFRVFSCKKAGSL 104
           IQTAVRL+LPGELAKHAVSEGTKAVTK+TSS   +F ++  K   +L
Sbjct: 91  IQTAVRLILPGELAKHAVSEGTKAVTKYTSSNGEQFTLYIVKMPNTL 137

Query: 105  164
           
Sbjct: 151  137

Query: 165  186
           
Sbjct: 211  137

[Close]

BLAST of CU100317 vs. TrEMBL
Match: G3NDH3_GASAC (Histone H2B OS=Gasterosteus aculeatus GN=HIST1H2BA PE=3 SV=1)

HSP 1 Score: 62.0 bits (149), Expect = 4.2e-07
Identity = 32/40 (80.00%), Postives = 35/40 (87.50%), Query Frame = 3


Query: 45  IQTAVRLVLPGELAKHAVSEGTKAVTKFTSS*IHRFRVFS 104
           IQTAVRL+LPGELAKHAVSEGTKAVTK+TSS + R   FS
Sbjct: 92  IQTAVRLLLPGELAKHAVSEGTKAVTKYTSSKLLRHSFFS 131

Query: 105  164
           
Sbjct: 152  131

[Close]

BLAST of CU100317 vs. TrEMBL
Match: A0A0B2F0X3_9BACL (Uncharacterized protein (Fragment) OS=Paenibacillus sp. IHB B 3415 GN=QW71_36400 PE=4 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 5.5e-07
Identity = 31/31 (100.00%), Postives = 31/31 (100.00%), Query Frame = 3


Query: 45  IQTAVRLVLPGELAKHAVSEGTKAVTKFTSS 104
           IQTAVRLVLPGELAKHAVSEGTKAVTKFTSS
Sbjct: 91  IQTAVRLVLPGELAKHAVSEGTKAVTKFTSS 121

Query: 105  138
           
Sbjct: 151  121

[Close]

BLAST of CU100317 vs. TrEMBL
Match: A0A087GCC9_ARAAL (Histone H2B OS=Arabis alpina GN=AALP_AA8G395500 PE=3 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 5.5e-07
Identity = 31/31 (100.00%), Postives = 31/31 (100.00%), Query Frame = 3


Query: 45  IQTAVRLVLPGELAKHAVSEGTKAVTKFTSS 104
           IQTAVRLVLPGELAKHAVSEGTKAVTKFTSS
Sbjct: 120 IQTAVRLVLPGELAKHAVSEGTKAVTKFTSS 150

Query: 105  138
           
Sbjct: 180  150

[Close]

BLAST of CU100317 vs. NCBI nr
Match: gi|655846865|ref|XP_008262727.1| (PREDICTED: histone H2B type 2-F-like [Oryctolagus cuniculus])

HSP 1 Score: 65.9 bits (159), Expect = 4.2e-08
Identity = 33/40 (82.50%), Postives = 36/40 (90.00%), Query Frame = 3


Query: 45  IQTAVRLVLPGELAKHAVSEGTKAVTKFTSS*IHRFRVFS 104
           IQTAVRL+LPGELAKHAVSEGTKAVTK+TSS I +F  FS
Sbjct: 95  IQTAVRLLLPGELAKHAVSEGTKAVTKYTSSNIKKFLFFS 134

Query: 105  164
           
Sbjct: 155  134

[Close]

BLAST of CU100317 vs. NCBI nr
Match: gi|913499544|ref|XP_013204527.1| (PREDICTED: uncharacterized protein LOC101998036 [Microtus ochrogaster])

HSP 1 Score: 65.5 bits (158), Expect = 5.5e-08
Identity = 34/52 (65.38%), Postives = 38/52 (73.08%), Query Frame = 3


Query: 45   IQTAVRLVLPGELAKHAVSEGTKAVTKFTSS*IHRFRVFSCKKAGSLVLDRG 104
            IQTAVRL+LPGELAKHAVSEGTKAVTK+TSS     R    +  G+ VL  G
Sbjct: 1425 IQTAVRLLLPGELAKHAVSEGTKAVTKYTSSKAFGLRARQARSLGAGVLGDG 1476

Query: 105   164
            
Sbjct: 1485  1476

Query: 165   201
            
Sbjct: 1545  1476

[Close]

BLAST of CU100317 vs. NCBI nr
Match: gi|602719525|ref|XP_007469932.1| (PREDICTED: IQ and AAA domain-containing protein 1-like [Lipotes vexillifer])

HSP 1 Score: 63.2 bits (152), Expect = 2.7e-07
Identity = 33/52 (63.46%), Postives = 36/52 (69.23%), Query Frame = 3


Query: 45  IQTAVRLVLPGELAKHAVSEGTKAVTKFTSS*IHRFRVFSCKKAGSLVLDRG 104
           IQTAVRL+LPGELAKHAVSEGTKAVTK+TSS         C    S + D G
Sbjct: 33  IQTAVRLLLPGELAKHAVSEGTKAVTKYTSS--------KCDSEASSICDSG 76

Query: 105  164
           
Sbjct: 93   76

Query: 165  201
           
Sbjct: 153  76

[Close]

BLAST of CU100317 vs. NCBI nr
Match: gi|743701785|ref|XP_010944072.1| (PREDICTED: uncharacterized protein LOC105061668 [Camelus bactrianus])

HSP 1 Score: 63.2 bits (152), Expect = 2.7e-07
Identity = 31/40 (77.50%), Postives = 34/40 (85.00%), Query Frame = 3


Query: 45  IQTAVRLVLPGELAKHAVSEGTKAVTKFTSS*IHRFRVFS 104
           IQTAVRL+LPGELAKHAVSEGTKAVTK+TSS +H     S
Sbjct: 486 IQTAVRLLLPGELAKHAVSEGTKAVTKYTSSNLHEIAEMS 525

Query: 105  164
           
Sbjct: 546  525

[Close]

BLAST of CU100317 vs. NCBI nr
Match: gi|609412460|pdb|4NFT|A (Chain A, Crystal Structure Of Human Lnkh2b-h2a.z-anp32e)

HSP 1 Score: 63.2 bits (152), Expect = 2.7e-07
Identity = 36/56 (64.29%), Postives = 38/56 (67.86%), Query Frame = 3


Query: 45  IQTAVRLVLPGELAKHAVSEGTKAVTKFTSS*IHRFRVFSCKKAGSLVLDRGFSFP 104
           IQTAVRL+LPGELAKHAVSEGTKAVTK+TSS          KKA S     G  FP
Sbjct: 66  IQTAVRLLLPGELAKHAVSEGTKAVTKYTSS----------KKAVSRSQRAGLQFP 111

Query: 105  164
           
Sbjct: 126  111

Query: 165  213
           
Sbjct: 186  111

The following BLAST results are available for this feature:

BLAST of CU100317 vs. Swiss-Prot


Match Name	E-value	Identity	Description
H2B3_ARATH	5.0e-09	100.00	Histone H2B.3 OS=Arabidopsis thaliana GN=At2g28720 PE=1 SV=3	[more]
H2B6_ORYSJ	5.0e-09	100.00	Histone H2B.6 OS=Oryza sativa subsp. japonica GN=H2B.6 PE=3 SV=1	[more]
H2B_GOSHI	5.0e-09	100.00	Histone H2B OS=Gossypium hirsutum GN=HIS2B PE=2 SV=3	[more]
H2B11_ARATH	5.0e-09	100.00	Histone H2B.11 OS=Arabidopsis thaliana GN=At5g59910 PE=1 SV=5	[more]
H2B_TOBAC	5.0e-09	100.00	Histone H2B OS=Nicotiana tabacum GN=HIS2B PE=2 SV=3	[more]

BLAST of CU100317 vs. TrEMBL


Match Name	E-value	Identity	Description
I3JKL4_ORENI	1.9e-07	79.49	Histone H2B OS=Oreochromis niloticus GN=HIST1H2BA PE=3 SV=1	[more]
A0A0N4VGK6_ENTVE	2.5e-07	68.09	Histone H4 OS=Enterobius vermicularis PE=3 SV=1	[more]
G3NDH3_GASAC	4.2e-07	80.00	Histone H2B OS=Gasterosteus aculeatus GN=HIST1H2BA PE=3 SV=1	[more]
A0A0B2F0X3_9BACL	5.5e-07	100.00	Uncharacterized protein (Fragment) OS=Paenibacillus sp. IHB B 3415 GN=QW71_36400...	[more]
A0A087GCC9_ARAAL	5.5e-07	100.00	Histone H2B OS=Arabis alpina GN=AALP_AA8G395500 PE=3 SV=1	[more]

BLAST of CU100317 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|655846865\|ref\|XP_008262727.1\|	4.2e-08	82.50	PREDICTED: histone H2B type 2-F-like [Oryctolagus cuniculus]	[more]
gi\|913499544\|ref\|XP_013204527.1\|	5.5e-08	65.38	PREDICTED: uncharacterized protein LOC101998036 [Microtus ochrogaster]	[more]
gi\|602719525\|ref\|XP_007469932.1\|	2.7e-07	63.46	PREDICTED: IQ and AAA domain-containing protein 1-like [Lipotes vexillifer]	[more]
gi\|743701785\|ref\|XP_010944072.1\|	2.7e-07	77.50	PREDICTED: uncharacterized protein LOC105061668 [Camelus bactrianus]	[more]
gi\|609412460\|pdb\|4NFT\|A	2.7e-07	64.29	Chain A, Crystal Structure Of Human Lnkh2b-h2a.z-anp32e	[more]

Annotated Terms

The following terms have been associated with this transcribed_cluster:

Vocabulary: **INTERPRO**
Term	Definition
IPR000558	Histone_H2B
IPR009072	Histone-fold

Vocabulary: **Cellular Component**
Term	Definition
GO:0000786	nucleosome

Vocabulary: **Molecular Function**
Term	Definition
GO:0003677	DNA binding
GO:0046982	protein heterodimerization activity

GO Annotation

GO Assignments

This transcribed_cluster is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0000786	nucleosome
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0046982	protein heterodimerization activity

Relationships

This transcribed_cluster is associated with the following gene feature(s):


Feature Name	Unique Name	Type
Csa1G132150	Csa1G132150	gene

The following EST feature(s) are a part of this transcribed_cluster:


Feature Name	Unique Name	Type
H0081578	H0081578	EST
H0174236	H0174236	EST

InterPro

Analysis Name: InterPro Annotations of cucumber unigene v3
Date Performed: 2016-11-16


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000558	Histone H2B	PRINTS	PR00621	HISTONEH2B	coord: 17..30 score: 5.8E-12coord: 30..43 score: 5.8
IPR000558	Histone H2B	PANTHER	PTHR23428	HISTONE H2B	coord: 19..46 score: 4.4
IPR009072	Histone-fold	GENE3D	G3DSA:1.10.20.10		coord: 1..46 score: 1.6
IPR009072	Histone-fold	unknown	SSF47113	Histone-fold	coord: 1..46 score: 5.69