MELO3C024886.2.1 (mRNA) Melon (DHL92) v3.6.1

NameMELO3C024886.2.1
TypemRNA
OrganismCucumis melo (Melon (DHL92) v3.6.1)
Description4-coumarate:CoA ligase
Locationchr01 : 11806353 .. 11810755 (-)
Sequence length2064
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTCCATTCTTCGAACTCAGAAGAACAATAAACCTTCAAGAAAAAAGAAAGTTATCTGTTCTTCTTCTTCTTCTTCTTCTTCTTCAATTCGGCGTTGAAATGGGAATTGAAACAGTTGAGAATGACGTTATTTTCCGGTCAAAACTACCGGACATCTACATCCCAAATCATCTTCCTCTGCATTCCTACTGCCTACAAGAAAACGCCGCCAAAATAGGCCACCGAACTTGCTTAATCAACGGCGTTACCGGCGAATCCTTCACCTTCAACGACGTCGATCTCACTGCACGCAAGGTCGCCTCCGGATTAAACAAACTCGGCATTACAAAACGCGACGTAATCATGCTCCTCCTCCCAAATTCACCGGAGTTCGTCTTCGCTTTCCTCGGGGCCTCCCACCTCGGCGCAATCATGACGGCCGCAAACCCTTTCTTCACGGCAGCAGAAATCGCAAAGCAAGCAAAAGGATCAAAAGCAAAACTAATCATAACGCAATCGTCCTACTACGAAAAGTTGAAGGAAATAACAGAGGAATTGCCAGAGGTTAAAATCATGACCGTCGATTCTCCTCACGATGGTTGTTTATGGTTCGGTGATCTAATTAAAGCCGACGAAAGAGAGATTCCGAGGGTGGAAATCGACCCAGATGACGTGGTTGCGCTGCCGTATTCGTCGGGAACGACGGGATTGCCAAAGGGAGTGATGCTTACACATAAAAGTTTGGTTACGAGTGTGGCTCAGCAAGTGGATGGGGAAAATCCAAATCTGTATTATGGAAATGAGGATGTGATTCTGTGTGTTTTGCCGTTGTTTCATATTTATTCTTTGAATTCAGTTTTGCTGTGTGGATTAAGAGCTGGAAGTACCATTTTGATTATGCCCAAATTTGAGATCGGTTTGCTTTTGCAATTGGTGGAGAAATATGGAGTTACCGTGGCGCCCATCGTGCCGCCCATCGTTCTGGCTATTGCAAAGTCGCCGGAGCTAGAGAAGTATGACCTTTCGTCGATCAGAGTTATTAAATCCGGCGGTGCACCGCTTGGGAAGGAGCTTGAAGATACCGTAAGAGCCAAGTTTCCTAAGGCTGTGCTTGGACAGGTATATCATCATGATACAAATTTATAATTTTAAGAACTTTAACAACAACCCTTTGGTTTTTTTGTTTTTAAAAATCAAGTTTCAAAAAACAAAAGAAAAAACTATAGAAAATATTAGATAGGTTTACAAAATAGTAAATATTATAAATATTGAAAATTTTCAATCACGGTTTAAGAATTTTAACTATTATAATGTGGTATCTTAACAGTAAATGTAAGAAAAATATATATTGAATTTGTTATACTCTCAATTTTTAATCACTGGTTTGGTTAAAGAACAATAAGAAACAAATATGTATATATAAAGAACAATTTTCGTTTGGTATTTTCAAAAAACCCGATTTTCATCCTTGTACAATCTACTAATTTTCTTTTAAATATATATATATTTTAAATTGTTTAGGTTTTAGAATGAGTTGCAACAAGATAATAGATGAAAAAATCTAAAGGTTTAATGGGTGGAGATTAGTGGTGTTATTAAATGTAATTAAGATTTTGTTATTAAATGTAATTAAGATTTTGTTATTAAATGTAATTAAGATTTTGTAGATGTGAAGGATAATTAGTCAAAATTCTTGATGTGAAGAAAGTCTTTGAGTGGAGACGAAATGACACCGAAGTCAAAGCAAAAGTCTGCGTGTAATTCTTTAGTTATTAAAGAAAAAATTAATTTAATTCATTTCTCATTATTAAGAATTATTTATTTTCATTTCCTAATTTGTTTTTGAAAGGTTTCGTCCACCTAAACAACAGCCCACCAACAACAAAACGCAAAACCAAGTTGGTAAACTTTTCACCTAAAACCTTTCACCAATTATTTTATATTTTTAAACACTTTTTTCTTTTTTTTACACTATTGATTAAATGATGAAAAACATTTTGACAATAATTTTATCAATGACAAAAGTAATTTATAAGATTCTAGGATCACTCCCTTTAAATCTCTTCTGTTTTTCTAACATCTTTATCAATTCACGAGGCTCCTATATAATAATAATAACAATAACAACACTAATAAAAAAGGTTAGGTTTCTTTTAAACATAAAATTCAAATTTATATCTAATATATTATATAATTTCTAGAATATCTTTAACATTTGGAAAAATTATTAAACAAAAATTTAGAATTTTAGTTTTCCTTCTTGTAGTTTTAACTTTTATATAATTAAGTATACAAACACCATCACCACACTTAATATCCACTTTTTACTTATATTTTGAAACAAAGTGGTTTACTATTTTAAAAAACAGATCAAAATACGAAATTATTATCAAATGAAATAAATTTATTAAAATTTTTTTAAAAATCATAAGTGTAATAAACCGAAAAGTTTATAGTATCTAATAAGTATCCTAGAAGATGAAATTTAGTTGTAAATATTTGTCTATCATAACCGAAAACTTTACAATAACTTACGTTTTCCATAAAATTACAACATTAAACTTCCGAGTGTTAAACTTTATATTTAATATTAAGATAAATATATTTGCCCACGTGGCATAACAATAATAACCGTTGGATGATTGTAGGGATACGGAATGACGGAGGCGGGTCCAGTACTAACAATGGGATTAGCATTTGCAAAGGAACCGTTTCCGATGAAACCAGGCGCCTGCGGCACCGTCGTCAGAAACGCCGAGATGAAGATCGTCGACACCGAAACCGGAAGTTCCTTGCCTCGTAACACGCCGGGAGAGATATGCATCAGAGGCGATCAAATCATGAAAGGCTATCTCAACAATCCGGAGGCAACGGCGGCCACAATCGACAAGGATGGGTGGTTGCACACCGGGGATATCGGATTCATCGACGAAGATGACGAGCTGTTCATTGTGGATCGGTTGAAGGAACTGATTAAGTACAAGGGATTTCAAGTGGCTCCGGCGGAACTGGAAGCTCTGTTGTTGACTCATCCGGTCATCTCTGATGCCGCCGTTGTTCCGTAAGAATAATGAATTGAATTTGGATACCGGCTGGGTGATGATTGTTGGGTTAATTTTGTGTGGATTGATATTGCAGAATGAAAGATGAACAAGCTGGAGAAGTTCCGGTCGCGTTCGTTGTGAAATTGAAGAATTCTGAAGCCACTGAAGATGAAATTAAACAATTTATTTCAAAACAGGTATCTGTTTTTTTTTTTACTTAACAAATTTGAATTCTATTTTTTAAAATTATTATCTGCTTCTTATAATTTTTTCTGAAATTATATTGACATCACATTTTATTGATATTTCTAGTCATTCTTGCAAAATTAAGATATTACAAATTGATGTTAGTTGGAGAATGCAATATTTATGCTAAAAGATTAATTATATATATATATATATATATATATATTATTTATTTTTTTTTTTTGTTGTTGTGTGTATATANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTTGTGTGTGTGGAATATTAATTAGGAATTTAAAATTAAAAAATACGTGCAGGTGGTTTTCTACAAAAAGATAAACCGGGTGTTTTTCATAGACGCAATTCCCAAATCGCCCTCGGGAAAGATCTTGAGGAAGGAGTTGAGGGCAAAGTTGGCAGCTGCATTTCCAAATTCTAATTAACTCTCTCTCTCTCTTCTTAATTTCACAACACACACACACACACTCAATGCTGCTAATGATGATGTAAGTTCTTCTTCTTCTTCATTTTTTTTTTTTTTAAATTTTAATTTTTAATTTTCCCTCTTGTTGTTTACTTGTTCATTTGGAAAACAAGAAAGTTGTATTTTACATTGGAATTTGTTGTACTTGTATGCAACTATCTAAAGACCAAGCTCTATATATACATAATATATAAAGAAGGTGACTTATTTCGAGTTTCGACTCGTATATGTGTATATTAATTACCCACTAATACTATTTTTACATTTTTTTCTTCTTTTGTATT

mRNA sequence

TTCCATTCTTCGAACTCAGAAGAACAATAAACCTTCAAGAAAAAAGAAAGTTATCTGTTCTTCTTCTTCTTCTTCTTCTTCTTCAATTCGGCGTTGAAATGGGAATTGAAACAGTTGAGAATGACGTTATTTTCCGGTCAAAACTACCGGACATCTACATCCCAAATCATCTTCCTCTGCATTCCTACTGCCTACAAGAAAACGCCGCCAAAATAGGCCACCGAACTTGCTTAATCAACGGCGTTACCGGCGAATCCTTCACCTTCAACGACGTCGATCTCACTGCACGCAAGGTCGCCTCCGGATTAAACAAACTCGGCATTACAAAACGCGACGTAATCATGCTCCTCCTCCCAAATTCACCGGAGTTCGTCTTCGCTTTCCTCGGGGCCTCCCACCTCGGCGCAATCATGACGGCCGCAAACCCTTTCTTCACGGCAGCAGAAATCGCAAAGCAAGCAAAAGGATCAAAAGCAAAACTAATCATAACGCAATCGTCCTACTACGAAAAGTTGAAGGAAATAACAGAGGAATTGCCAGAGGTTAAAATCATGACCGTCGATTCTCCTCACGATGGTTGTTTATGGTTCGGTGATCTAATTAAAGCCGACGAAAGAGAGATTCCGAGGGTGGAAATCGACCCAGATGACGTGGTTGCGCTGCCGTATTCGTCGGGAACGACGGGATTGCCAAAGGGAGTGATGCTTACACATAAAAGTTTGGTTACGAGTGTGGCTCAGCAAGTGGATGGGGAAAATCCAAATCTGTATTATGGAAATGAGGATGTGATTCTGTGTGTTTTGCCGTTGTTTCATATTTATTCTTTGAATTCAGTTTTGCTGTGTGGATTAAGAGCTGGAAGTACCATTTTGATTATGCCCAAATTTGAGATCGGTTTGCTTTTGCAATTGGTGGAGAAATATGGAGTTACCGTGGCGCCCATCGTGCCGCCCATCGTTCTGGCTATTGCAAAGTCGCCGGAGCTAGAGAAGTATGACCTTTCGTCGATCAGAGTTATTAAATCCGGCGGTGCACCGCTTGGGAAGGAGCTTGAAGATACCGTAAGAGCCAAGTTTCCTAAGGCTGTGCTTGGACAGGGATACGGAATGACGGAGGCGGGTCCAGTACTAACAATGGGATTAGCATTTGCAAAGGAACCGTTTCCGATGAAACCAGGCGCCTGCGGCACCGTCGTCAGAAACGCCGAGATGAAGATCGTCGACACCGAAACCGGAAGTTCCTTGCCTCGTAACACGCCGGGAGAGATATGCATCAGAGGCGATCAAATCATGAAAGGCTATCTCAACAATCCGGAGGCAACGGCGGCCACAATCGACAAGGATGGGTGGTTGCACACCGGGGATATCGGATTCATCGACGAAGATGACGAGCTGTTCATTGTGGATCGGTTGAAGGAACTGATTAAGTACAAGGGATTTCAAGTGGCTCCGGCGGAACTGGAAGCTCTGTTGTTGACTCATCCGGTCATCTCTGATGCCGCCGTTGTTCCAATGAAAGATGAACAAGCTGGAGAAGTTCCGGTCGCGTTCGTTGTGAAATTGAAGAATTCTGAAGCCACTGAAGATGAAATTAAACAATTTATTTCAAAACAGGTGGTTTTCTACAAAAAGATAAACCGGGTGTTTTTCATAGACGCAATTCCCAAATCGCCCTCGGGAAAGATCTTGAGGAAGGAGTTGAGGGCAAAGTTGGCAGCTGCATTTCCAAATTCTAATTAACTCTCTCTCTCTCTTCTTAATTTCACAACACACACACACACACTCAATGCTGCTAATGATGATGTAAGTTCTTCTTCTTCTTCATTTTTTTTTTTTTTAaATTTTAATTTTTAATTTTCCCTCTTGTTGTTTACTTGTTCATTTGGAAAACAAGAAAGTTGTATTTTACATTGGAATTTGTTGTACTTGTATGCAACTATCTAAAGACCAAGCTCTATATATACATAATATATAAAGAAGGTGACTTATTTCGAGTTTCGACTCGTATATGTGTATATTAATTACCCACTAATACTATTTTTACATTTTTTTCTTCTTTTGTATT

Coding sequence (CDS)

ATGGGAATTGAAACAGTTGAGAATGACGTTATTTTCCGGTCAAAACTACCGGACATCTACATCCCAAATCATCTTCCTCTGCATTCCTACTGCCTACAAGAAAACGCCGCCAAAATAGGCCACCGAACTTGCTTAATCAACGGCGTTACCGGCGAATCCTTCACCTTCAACGACGTCGATCTCACTGCACGCAAGGTCGCCTCCGGATTAAACAAACTCGGCATTACAAAACGCGACGTAATCATGCTCCTCCTCCCAAATTCACCGGAGTTCGTCTTCGCTTTCCTCGGGGCCTCCCACCTCGGCGCAATCATGACGGCCGCAAACCCTTTCTTCACGGCAGCAGAAATCGCAAAGCAAGCAAAAGGATCAAAAGCAAAACTAATCATAACGCAATCGTCCTACTACGAAAAGTTGAAGGAAATAACAGAGGAATTGCCAGAGGTTAAAATCATGACCGTCGATTCTCCTCACGATGGTTGTTTATGGTTCGGTGATCTAATTAAAGCCGACGAAAGAGAGATTCCGAGGGTGGAAATCGACCCAGATGACGTGGTTGCGCTGCCGTATTCGTCGGGAACGACGGGATTGCCAAAGGGAGTGATGCTTACACATAAAAGTTTGGTTACGAGTGTGGCTCAGCAAGTGGATGGGGAAAATCCAAATCTGTATTATGGAAATGAGGATGTGATTCTGTGTGTTTTGCCGTTGTTTCATATTTATTCTTTGAATTCAGTTTTGCTGTGTGGATTAAGAGCTGGAAGTACCATTTTGATTATGCCCAAATTTGAGATCGGTTTGCTTTTGCAATTGGTGGAGAAATATGGAGTTACCGTGGCGCCCATCGTGCCGCCCATCGTTCTGGCTATTGCAAAGTCGCCGGAGCTAGAGAAGTATGACCTTTCGTCGATCAGAGTTATTAAATCCGGCGGTGCACCGCTTGGGAAGGAGCTTGAAGATACCGTAAGAGCCAAGTTTCCTAAGGCTGTGCTTGGACAGGGATACGGAATGACGGAGGCGGGTCCAGTACTAACAATGGGATTAGCATTTGCAAAGGAACCGTTTCCGATGAAACCAGGCGCCTGCGGCACCGTCGTCAGAAACGCCGAGATGAAGATCGTCGACACCGAAACCGGAAGTTCCTTGCCTCGTAACACGCCGGGAGAGATATGCATCAGAGGCGATCAAATCATGAAAGGCTATCTCAACAATCCGGAGGCAACGGCGGCCACAATCGACAAGGATGGGTGGTTGCACACCGGGGATATCGGATTCATCGACGAAGATGACGAGCTGTTCATTGTGGATCGGTTGAAGGAACTGATTAAGTACAAGGGATTTCAAGTGGCTCCGGCGGAACTGGAAGCTCTGTTGTTGACTCATCCGGTCATCTCTGATGCCGCCGTTGTTCCAATGAAAGATGAACAAGCTGGAGAAGTTCCGGTCGCGTTCGTTGTGAAATTGAAGAATTCTGAAGCCACTGAAGATGAAATTAAACAATTTATTTCAAAACAGGTGGTTTTCTACAAAAAGATAAACCGGGTGTTTTTCATAGACGCAATTCCCAAATCGCCCTCGGGAAAGATCTTGAGGAAGGAGTTGAGGGCAAAGTTGGCAGCTGCATTTCCAAATTCTAATTAA

Protein sequence

MGIETVENDVIFRSKLPDIYIPNHLPLHSYCLQENAAKIGHRTCLINGVTGESFTFNDVDLTARKVASGLNKLGITKRDVIMLLLPNSPEFVFAFLGASHLGAIMTAANPFFTAAEIAKQAKGSKAKLIITQSSYYEKLKEITEELPEVKIMTVDSPHDGCLWFGDLIKADEREIPRVEIDPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGNEDVILCVLPLFHIYSLNSVLLCGLRAGSTILIMPKFEIGLLLQLVEKYGVTVAPIVPPIVLAIAKSPELEKYDLSSIRVIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPGACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHTGDIGFIDEDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVPVAFVVKLKNSEATEDEIKQFISKQVVFYKKINRVFFIDAIPKSPSGKILRKELRAKLAAAFPNSN
BLAST of MELO3C024886.2.1 vs. NCBI nr
Match: XP_004137646.2 (PREDICTED: 4-coumarate--CoA ligase 1 [Cucumis sativus])

HSP 1 Score: 1050.8 bits (2716), Expect = 1.6e-303
Identity = 526/546 (96.34%), Postives = 540/546 (98.90%), Query Frame = 0

Query: 1   MGIETVENDVIFRSKLPDIYIPNHLPLHSYCLQENAAKIGHRTCLINGVTGESFTFNDVD 60
           MGIETVENDVIFRSKLPDIYIP HLPLHSYCLQENAAKIGHRTCLINGVTGESFT+NDVD
Sbjct: 1   MGIETVENDVIFRSKLPDIYIPKHLPLHSYCLQENAAKIGHRTCLINGVTGESFTYNDVD 60

Query: 61  LTARKVASGLNKLGITKRDVIMLLLPNSPEFVFAFLGASHLGAIMTAANPFFTAAEIAKQ 120
           LT RKVASGLNKLGITKRDVIMLLLPNSPEFVFAFLGAS+LGAIMTAANPFFTAAEI KQ
Sbjct: 61  LTTRKVASGLNKLGITKRDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIGKQ 120

Query: 121 AKGSKAKLIITQSSYYEKLKEITEELPEVKIMTVDSPHDGCLWFGDLIKADEREIPRVEI 180
           AKGSK+KLIITQSSYYEK+KEITEELPEVKIMTVDSP DGCLWFGDLIKADERE+PRV+I
Sbjct: 121 AKGSKSKLIITQSSYYEKVKEITEELPEVKIMTVDSPPDGCLWFGDLIKADEREVPRVDI 180

Query: 181 DPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGNEDVILCVLPLFHI 240
           DP+DVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYG+EDVILCVLPLFHI
Sbjct: 181 DPEDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGHEDVILCVLPLFHI 240

Query: 241 YSLNSVLLCGLRAGSTILIMPKFEIGLLLQLVEKYGVTVAPIVPPIVLAIAKSPELEKYD 300
           YSLNSVLLCGLRAGSTILIMPKFEIGLLLQL EKYGVTVAPIVPPIVLAIAKSPELEKYD
Sbjct: 241 YSLNSVLLCGLRAGSTILIMPKFEIGLLLQLAEKYGVTVAPIVPPIVLAIAKSPELEKYD 300

Query: 301 LSSIRVIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360
           LSSIR+IKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAK+PFPMKPG
Sbjct: 301 LSSIRIIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKDPFPMKPG 360

Query: 361 ACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420
           ACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT
Sbjct: 361 ACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420

Query: 421 GDIGFIDEDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480
           GDIGFID+DDE+FIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV
Sbjct: 421 GDIGFIDDDDEMFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480

Query: 481 PVAFVVKLKNSEATEDEIKQFISKQVVFYKKINRVFFIDAIPKSPSGKILRKELRAKLAA 540
           PVAFVVKLKNSEA+EDEIKQFISKQVVFYK+INRVFFIDAIPKSPSGKILRKELRAKLAA
Sbjct: 481 PVAFVVKLKNSEASEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAA 540

Query: 541 AFPNSN 547
           AFP SN
Sbjct: 541 AFPTSN 546

BLAST of MELO3C024886.2.1 vs. NCBI nr
Match: XP_008462955.1 (PREDICTED: 4-coumarate--CoA ligase 1-like [Cucumis melo])

HSP 1 Score: 1004.6 bits (2596), Expect = 1.3e-289
Identity = 510/519 (98.27%), Postives = 513/519 (98.84%), Query Frame = 0

Query: 1   MGIETVENDVIFRSKLPDIYIPNHLPLHSYCLQENAAKIGHRTCLINGVTGESFTFNDVD 60
           MGIETVENDVIFRSKLPDIYIPNHLPLHSYCLQENAAKIGHRTCLINGVTGESFTFNDVD
Sbjct: 1   MGIETVENDVIFRSKLPDIYIPNHLPLHSYCLQENAAKIGHRTCLINGVTGESFTFNDVD 60

Query: 61  LTARKVASGLNKLGITKRDVIMLLLPNSPEFVFAFLGASHLGAIMTAANPFFTAAEIAKQ 120
           LTARKVASGLNKLGITKRDVIMLLLPNSPEFVFAFLGASHLGAIMTAANPFFTAAEIAKQ
Sbjct: 61  LTARKVASGLNKLGITKRDVIMLLLPNSPEFVFAFLGASHLGAIMTAANPFFTAAEIAKQ 120

Query: 121 AKGSKAKLIITQSSYYEKLKEITEELPEVKIMTVDSPHDGCLWFGDLIKADEREIPRVEI 180
           AKGSKAKLIITQSSYYEKLKEITEELPEVKIMTVDSPHDGCLWFGDLIKADEREIPRVEI
Sbjct: 121 AKGSKAKLIITQSSYYEKLKEITEELPEVKIMTVDSPHDGCLWFGDLIKADEREIPRVEI 180

Query: 181 DPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGNEDVILCVLPLFHI 240
           DPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGNEDVILCVLPLFHI
Sbjct: 181 DPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGNEDVILCVLPLFHI 240

Query: 241 YSLNSVLLCGLRAGSTILIMPKFEIGLLLQLVEKYGVTVAPIVPPIVLAIAKSPELEKYD 300
           YSLNSVLLCGLRAGSTILIMPKFEIGLLLQLVEKYGVTVAPIVPPIVLAIAKSPELEKYD
Sbjct: 241 YSLNSVLLCGLRAGSTILIMPKFEIGLLLQLVEKYGVTVAPIVPPIVLAIAKSPELEKYD 300

Query: 301 LSSIRVIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360
           LSSIRVIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG
Sbjct: 301 LSSIRVIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360

Query: 361 ACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420
           ACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT
Sbjct: 361 ACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420

Query: 421 GDIGFIDEDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480
           GDIGFIDEDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV
Sbjct: 421 GDIGFIDEDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480

Query: 481 PVAFVVKLKNSEATEDEIKQFISKQV-VFY--KKINRVF 517
           PVAFVVKLKNSEATEDEIKQFISKQV VF+    +N +F
Sbjct: 481 PVAFVVKLKNSEATEDEIKQFISKQVSVFFYLTNLNSIF 519

BLAST of MELO3C024886.2.1 vs. NCBI nr
Match: XP_022935389.1 (4-coumarate--CoA ligase 1-like [Cucurbita moschata])

HSP 1 Score: 981.9 bits (2537), Expect = 9.0e-283
Identity = 490/546 (89.74%), Postives = 517/546 (94.69%), Query Frame = 0

Query: 1   MGIETVENDVIFRSKLPDIYIPNHLPLHSYCLQENAAKIGHRTCLINGVTGESFTFNDVD 60
           M +E ++ND IFRSKLPDIYIPNHLPLHSYCL EN AKIGHRTCLIN VTGESFT++DVD
Sbjct: 1   MAVEALQNDFIFRSKLPDIYIPNHLPLHSYCLHENLAKIGHRTCLINSVTGESFTYHDVD 60

Query: 61  LTARKVASGLNKLGITKRDVIMLLLPNSPEFVFAFLGASHLGAIMTAANPFFTAAEIAKQ 120
           L ARKVASGLNKLGI + DVIML+L NSPEFVFAFLGAS+ GAIMTAANPFFTAAEIAKQ
Sbjct: 61  LAARKVASGLNKLGIAQGDVIMLILQNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120

Query: 121 AKGSKAKLIITQSSYYEKLKEITEELPEVKIMTVDSPHDGCLWFGDLIKADEREIPRVEI 180
           AKGS AKLI+TQSSYYEK+KEITEELP+VKIMTVDSP DGCL F DLI+ADERE+P VEI
Sbjct: 121 AKGSNAKLIVTQSSYYEKVKEITEELPDVKIMTVDSPPDGCLSFADLIQADEREMPTVEI 180

Query: 181 DPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGNEDVILCVLPLFHI 240
           +PDDVVALPYSSGTTGLPKGVMLTH+ LVTSVAQQVDGENPNLYY NEDVILCVLPLFHI
Sbjct: 181 NPDDVVALPYSSGTTGLPKGVMLTHRGLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHI 240

Query: 241 YSLNSVLLCGLRAGSTILIMPKFEIGLLLQLVEKYGVTVAPIVPPIVLAIAKSPELEKYD 300
           YSLNSVLLCGLRAG+TILIMPKFEIG LLQLV+K+ V++APIVPPIVLAIAKSP+L+KYD
Sbjct: 241 YSLNSVLLCGLRAGTTILIMPKFEIGSLLQLVQKFKVSIAPIVPPIVLAIAKSPDLDKYD 300

Query: 301 LSSIRVIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360
           LSSIRVIK GGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG
Sbjct: 301 LSSIRVIKCGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360

Query: 361 ACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420
           ACGTVVRNAEMKIVDTE G SLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT
Sbjct: 361 ACGTVVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420

Query: 421 GDIGFIDEDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480
           GDIGFID+DDELFIVDRLKELIKYKGFQVAPAE+EALLLTHP ISDAAVVPMKDE+AGEV
Sbjct: 421 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAEVEALLLTHPAISDAAVVPMKDEEAGEV 480

Query: 481 PVAFVVKLKNSEATEDEIKQFISKQVVFYKKINRVFFIDAIPKSPSGKILRKELRAKLAA 540
           PVAFVV+LKNSE TEDEIKQFISKQVVFYK+I R F IDAIPKSPSGKILRKELRAKLAA
Sbjct: 481 PVAFVVRLKNSEVTEDEIKQFISKQVVFYKRIKRAFMIDAIPKSPSGKILRKELRAKLAA 540

Query: 541 AFPNSN 547
            FPNSN
Sbjct: 541 GFPNSN 546

BLAST of MELO3C024886.2.1 vs. NCBI nr
Match: XP_023526871.1 (4-coumarate--CoA ligase 1-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 975.7 bits (2521), Expect = 6.5e-281
Identity = 487/546 (89.19%), Postives = 514/546 (94.14%), Query Frame = 0

Query: 1   MGIETVENDVIFRSKLPDIYIPNHLPLHSYCLQENAAKIGHRTCLINGVTGESFTFNDVD 60
           M +E ++ND IFRSKLP IYIPNHLPLHSYCL EN AKIGHRTCLIN VTGESFT++DVD
Sbjct: 1   MAVEALQNDFIFRSKLPHIYIPNHLPLHSYCLHENLAKIGHRTCLINSVTGESFTYHDVD 60

Query: 61  LTARKVASGLNKLGITKRDVIMLLLPNSPEFVFAFLGASHLGAIMTAANPFFTAAEIAKQ 120
           L ARKVASGLNKLGI + DVIMLLL NSPEFVFAFLGAS+ GAIMTAANPFFTAAEIAKQ
Sbjct: 61  LAARKVASGLNKLGIAQGDVIMLLLQNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120

Query: 121 AKGSKAKLIITQSSYYEKLKEITEELPEVKIMTVDSPHDGCLWFGDLIKADEREIPRVEI 180
           AKGS AKLI+TQSSYYEK+KEITEE+P+VKIMTVDSP DGCL F DLI+ADERE+P VEI
Sbjct: 121 AKGSNAKLIVTQSSYYEKVKEITEEVPDVKIMTVDSPPDGCLSFADLIQADEREMPTVEI 180

Query: 181 DPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGNEDVILCVLPLFHI 240
           +PDDVVALPYSSGTTGLPKGVMLTH+ LVTSVAQQVDGENPNLYY NEDVILCVLPLFHI
Sbjct: 181 NPDDVVALPYSSGTTGLPKGVMLTHRGLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHI 240

Query: 241 YSLNSVLLCGLRAGSTILIMPKFEIGLLLQLVEKYGVTVAPIVPPIVLAIAKSPELEKYD 300
           YSLNSVLLCGLRAG+TILIMPKFEIG LLQLV+K+ V++APIVPPIVLAIAKSP+L+KYD
Sbjct: 241 YSLNSVLLCGLRAGTTILIMPKFEIGSLLQLVQKFKVSIAPIVPPIVLAIAKSPDLDKYD 300

Query: 301 LSSIRVIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360
           LSSIRVIK GGAPLGKELEDTVR KFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG
Sbjct: 301 LSSIRVIKCGGAPLGKELEDTVRTKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360

Query: 361 ACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420
           ACGTVVRNAEMKIVDTE G SLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT
Sbjct: 361 ACGTVVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420

Query: 421 GDIGFIDEDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480
           GDIGFID+DDELFIVDRLKELIKYKGFQVAPAE+EALLLTHP ISDAAVVPMKDE+AGEV
Sbjct: 421 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAEVEALLLTHPAISDAAVVPMKDEEAGEV 480

Query: 481 PVAFVVKLKNSEATEDEIKQFISKQVVFYKKINRVFFIDAIPKSPSGKILRKELRAKLAA 540
           PVAFVV+LKNSE TEDEIKQFISKQVVFYK+I R F IDAIPKSPSGKILRKELRAKLA 
Sbjct: 481 PVAFVVRLKNSEVTEDEIKQFISKQVVFYKRIKRAFMIDAIPKSPSGKILRKELRAKLAT 540

Query: 541 AFPNSN 547
            FPNSN
Sbjct: 541 GFPNSN 546

BLAST of MELO3C024886.2.1 vs. NCBI nr
Match: XP_022983944.1 (4-coumarate--CoA ligase 1-like [Cucurbita maxima])

HSP 1 Score: 973.4 bits (2515), Expect = 3.2e-280
Identity = 487/546 (89.19%), Postives = 514/546 (94.14%), Query Frame = 0

Query: 1   MGIETVENDVIFRSKLPDIYIPNHLPLHSYCLQENAAKIGHRTCLINGVTGESFTFNDVD 60
           M +E ++ND IFRSKLPDIYIPNHLPLHSYCL EN AKIGHRTCLIN VT ESFT++DVD
Sbjct: 1   MAVEALQNDFIFRSKLPDIYIPNHLPLHSYCLHENLAKIGHRTCLINAVTAESFTYHDVD 60

Query: 61  LTARKVASGLNKLGITKRDVIMLLLPNSPEFVFAFLGASHLGAIMTAANPFFTAAEIAKQ 120
           L ARKVASGLNKLGI + DVI+LLL NSPEFVFAFLGAS+ GAIMTAANPFFTAAEIAKQ
Sbjct: 61  LAARKVASGLNKLGIAQGDVILLLLQNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120

Query: 121 AKGSKAKLIITQSSYYEKLKEITEELPEVKIMTVDSPHDGCLWFGDLIKADEREIPRVEI 180
           AKGS AKLI+TQSSYYEK+KEITEELP+VKIMTVDSP DGCL F DLI+ADERE+P VEI
Sbjct: 121 AKGSNAKLIVTQSSYYEKVKEITEELPDVKIMTVDSPPDGCLSFADLIQADEREMPTVEI 180

Query: 181 DPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGNEDVILCVLPLFHI 240
           +PDDVVALPYSSGTTGLPKGVMLTH+ LVTSVAQQVDGENPNLYY NEDVILCVLPLFHI
Sbjct: 181 NPDDVVALPYSSGTTGLPKGVMLTHRGLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHI 240

Query: 241 YSLNSVLLCGLRAGSTILIMPKFEIGLLLQLVEKYGVTVAPIVPPIVLAIAKSPELEKYD 300
           YSLNSVLLCGLRAG+TILIMPKFEIG LLQLV+K+ V++APIVPPIVLAIAKSP+L+KYD
Sbjct: 241 YSLNSVLLCGLRAGTTILIMPKFEIGSLLQLVQKFKVSIAPIVPPIVLAIAKSPDLDKYD 300

Query: 301 LSSIRVIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360
           LSSIRVIK GGAPLGKELED+VRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG
Sbjct: 301 LSSIRVIKCGGAPLGKELEDSVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360

Query: 361 ACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420
           ACGTVVRNAEMKIVDTE G SLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT
Sbjct: 361 ACGTVVRNAEMKIVDTENGCSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420

Query: 421 GDIGFIDEDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480
           GDIGFID+DDELFIVDRLKELIKYKGFQVAPAELEALLLTHP ISDAAVVPMKDE+AGEV
Sbjct: 421 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPAISDAAVVPMKDEEAGEV 480

Query: 481 PVAFVVKLKNSEATEDEIKQFISKQVVFYKKINRVFFIDAIPKSPSGKILRKELRAKLAA 540
           PVAFVV+LKNSE TEDEIKQFISKQVVFYK+I R F IDAIPKSPSGKILRKELRAKLA 
Sbjct: 481 PVAFVVRLKNSEVTEDEIKQFISKQVVFYKRIKRAFMIDAIPKSPSGKILRKELRAKLAT 540

Query: 541 AFPNSN 547
            FP SN
Sbjct: 541 GFPISN 546

BLAST of MELO3C024886.2.1 vs. TAIR10
Match: AT3G21240.1 (4-coumarate:CoA ligase 2)

HSP 1 Score: 766.9 bits (1979), Expect = 8.3e-222
Identity = 382/541 (70.61%), Postives = 455/541 (84.10%), Query Frame = 0

Query: 8   NDVIFRSKLPDIYIPNHLPLHSYCLQENAAKIGHRTCLINGVTGESFTFNDVDLTARKVA 67
           NDVIFRS+LPDIYIPNHLPLH Y + EN ++   + CLING TGE +T+ DV +T+RK+A
Sbjct: 19  NDVIFRSRLPDIYIPNHLPLHDY-IFENISEFAAKPCLINGPTGEVYTYADVHVTSRKLA 78

Query: 68  SGLNKLGITKRDVIMLLLPNSPEFVFAFLGASHLGAIMTAANPFFTAAEIAKQAKGSKAK 127
           +GL+ LG+ + DV+M+LLPNSPE V  FL AS +GAI T+ANPFFT AEI+KQAK S AK
Sbjct: 79  AGLHNLGVKQHDVVMILLPNSPEVVLTFLAASFIGAITTSANPFFTPAEISKQAKASAAK 138

Query: 128 LIITQSSYYEKLKEITEELPEVKIMTVDSP--HDGCLWFGDLIKADEREIPRV--EIDPD 187
           LI+TQS Y +K+K +  +   V I+T DS    + CL F +L +++E  +  +  +I P+
Sbjct: 139 LIVTQSRYVDKIKNLQND--GVLIVTTDSDAIPENCLRFSELTQSEEPRVDSIPEKISPE 198

Query: 188 DVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGNEDVILCVLPLFHIYSL 247
           DVVALP+SSGTTGLPKGVMLTHK LVTSVAQQVDGENPNLY+  +DVILCVLP+FHIY+L
Sbjct: 199 DVVALPFSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFNRDDVILCVLPMFHIYAL 258

Query: 248 NSVLLCGLRAGSTILIMPKFEIGLLLQLVEKYGVTVAPIVPPIVLAIAKSPELEKYDLSS 307
           NS++LC LR G+TILIMPKFEI LLL+ +++  VTVA +VPPIVLAIAKSPE EKYDLSS
Sbjct: 259 NSIMLCSLRVGATILIMPKFEITLLLEQIQRCKVTVAMVVPPIVLAIAKSPETEKYDLSS 318

Query: 308 IRVIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPGACG 367
           +R++KSG APLGKELED + AKFP A LGQGYGMTEAGPVL M L FAKEPFP+K GACG
Sbjct: 319 VRMVKSGAAPLGKELEDAISAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKSGACG 378

Query: 368 TVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHTGDI 427
           TVVRNAEMKI+D +TG SLPRN PGEICIRG+QIMKGYLN+P ATA+TIDKDGWLHTGD+
Sbjct: 379 TVVRNAEMKILDPDTGDSLPRNKPGEICIRGNQIMKGYLNDPLATASTIDKDGWLHTGDV 438

Query: 428 GFIDEDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVPVA 487
           GFID+DDELFIVDRLKELIKYKGFQVAPAELE+LL+ HP I+D AVV MK+E AGEVPVA
Sbjct: 439 GFIDDDDELFIVDRLKELIKYKGFQVAPAELESLLIGHPEINDVAVVAMKEEDAGEVPVA 498

Query: 488 FVVKLKNSEATEDEIKQFISKQVVFYKKINRVFFIDAIPKSPSGKILRKELRAKLAAAFP 545
           FVV+ K+S  +EDEIKQF+SKQVVFYK+IN+VFF D+IPK+PSGKILRK+LRA+LA    
Sbjct: 499 FVVRSKDSNISEDEIKQFVSKQVVFYKRINKVFFTDSIPKAPSGKILRKDLRARLANGLM 556

BLAST of MELO3C024886.2.1 vs. TAIR10
Match: AT1G51680.1 (4-coumarate:CoA ligase 1)

HSP 1 Score: 757.7 bits (1955), Expect = 5.0e-219
Identity = 385/540 (71.30%), Postives = 445/540 (82.41%), Query Frame = 0

Query: 8   NDVIFRSKLPDIYIPNHLPLHSYCLQENAAKIGHRTCLINGVTGESFTFNDVDLTARKVA 67
           +DVIFRSKLPDIYIPNHL LH Y  Q N ++   + CLING TG  +T++DV + +R++A
Sbjct: 21  SDVIFRSKLPDIYIPNHLSLHDYIFQ-NISEFATKPCLINGPTGHVYTYSDVHVISRQIA 80

Query: 68  SGLNKLGITKRDVIMLLLPNSPEFVFAFLGASHLGAIMTAANPFFTAAEIAKQAKGSKAK 127
           +  +KLG+ + DV+MLLLPN PEFV +FL AS  GA  TAANPFFT AEIAKQAK S  K
Sbjct: 81  ANFHKLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAKASNTK 140

Query: 128 LIITQSSYYEKLKEITEELPEVKIMTVDSPH-----DGCLWFGDLIKAD---EREIPRVE 187
           LIIT++ Y +K+K +  +   V I+ +D        +GCL F +L ++       I  VE
Sbjct: 141 LIITEARYVDKIKPLQND-DGVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEVIDSVE 200

Query: 188 IDPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGNEDVILCVLPLFH 247
           I PDDVVALPYSSGTTGLPKGVMLTHK LVTSVAQQVDGENPNLY+ ++DVILCVLP+FH
Sbjct: 201 ISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLPMFH 260

Query: 248 IYSLNSVLLCGLRAGSTILIMPKFEIGLLLQLVEKYGVTVAPIVPPIVLAIAKSPELEKY 307
           IY+LNS++LCGLR G+ ILIMPKFEI LLL+L+++  VTVAP+VPPIVLAIAKS E EKY
Sbjct: 261 IYALNSIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAKSSETEKY 320

Query: 308 DLSSIRVIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKP 367
           DLSSIRV+KSG APLGKELED V AKFP A LGQGYGMTEAGPVL M L FAKEPFP+K 
Sbjct: 321 DLSSIRVVKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKS 380

Query: 368 GACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLH 427
           GACGTVVRNAEMKIVD +TG SL RN PGEICIRG QIMKGYLNNP ATA TIDKDGWLH
Sbjct: 381 GACGTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKGYLNNPAATAETIDKDGWLH 440

Query: 428 TGDIGFIDEDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGE 487
           TGDIG ID+DDELFIVDRLKELIKYKGFQVAPAELEALL+ HP I+D AVV MK+E AGE
Sbjct: 441 TGDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVVAMKEEAAGE 500

Query: 488 VPVAFVVKLKNSEATEDEIKQFISKQVVFYKKINRVFFIDAIPKSPSGKILRKELRAKLA 540
           VPVAFVVK K+SE +ED++KQF+SKQVVFYK+IN+VFF ++IPK+PSGKILRK+LRAKLA
Sbjct: 501 VPVAFVVKSKDSELSEDDVKQFVSKQVVFYKRINKVFFTESIPKAPSGKILRKDLRAKLA 558

BLAST of MELO3C024886.2.1 vs. TAIR10
Match: AT1G65060.1 (4-coumarate:CoA ligase 3)

HSP 1 Score: 686.8 bits (1771), Expect = 1.1e-197
Identity = 342/531 (64.41%), Postives = 427/531 (80.41%), Query Frame = 0

Query: 11  IFRSKLPDIYIPNHLPLHSYCLQENAAKIGHRTCLINGVTGESFTFNDVDLTARKVASGL 70
           IFRSKLPDI IPNHLPLH+YC  E  + +  + CLI G TG+S+T+ +  L  R+VASGL
Sbjct: 34  IFRSKLPDIDIPNHLPLHTYCF-EKLSSVSDKPCLIVGSTGKSYTYGETHLICRRVASGL 93

Query: 71  NKLGITKRDVIMLLLPNSPEFVFAFLGASHLGAIMTAANPFFTAAEIAKQAKGSKAKLII 130
            KLGI K DVIM+LL NS EFVF+F+GAS +GA+ T ANPF+T+ E+ KQ K S AKLII
Sbjct: 94  YKLGIRKGDVIMILLQNSAEFVFSFMGASMIGAVSTTANPFYTSQELYKQLKSSGAKLII 153

Query: 131 TQSSYYEKLKEITEELPEVKIMTVDSP-HDGCLWFGDLIKADERE--IPRVEIDPDDVVA 190
           T S Y +KLK + E L    ++T D P  + CL F  LI  DE       V+I  DD  A
Sbjct: 154 THSQYVDKLKNLGENL---TLITTDEPTPENCLPFSTLITDDETNPFQETVDIGGDDAAA 213

Query: 191 LPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGNEDVILCVLPLFHIYSLNSVL 250
           LP+SSGTTGLPKGV+LTHKSL+TSVAQQVDG+NPNLY  + DVILCVLPLFHIYSLNSVL
Sbjct: 214 LPFSSGTTGLPKGVVLTHKSLITSVAQQVDGDNPNLYLKSNDVILCVLPLFHIYSLNSVL 273

Query: 251 LCGLRAGSTILIMPKFEIGLLLQLVEKYGVTVAPIVPPIVLAIAKSPELEKYDLSSIRVI 310
           L  LR+G+T+L+M KFEIG LL L++++ VT+A +VPP+V+A+AK+P +  YDLSS+R +
Sbjct: 274 LNSLRSGATVLLMHKFEIGALLDLIQRHRVTIAALVPPLVIALAKNPTVNSYDLSSVRFV 333

Query: 311 KSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPGACGTVVR 370
            SG APLGKEL+D++R + P+A+LGQGYGMTEAGPVL+M L FAKEP P K G+CGTVVR
Sbjct: 334 LSGAAPLGKELQDSLRRRLPQAILGQGYGMTEAGPVLSMSLGFAKEPIPTKSGSCGTVVR 393

Query: 371 NAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHTGDIGFID 430
           NAE+K+V  ET  SL  N PGEICIRG QIMK YLN+PEAT+ATID++GWLHTGDIG++D
Sbjct: 394 NAELKVVHLETRLSLGYNQPGEICIRGQQIMKEYLNDPEATSATIDEEGWLHTGDIGYVD 453

Query: 431 EDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVPVAFVVK 490
           EDDE+FIVDRLKE+IK+KGFQV PAELE+LL+ H  I+DAAVVP  DE AGEVPVAFVV+
Sbjct: 454 EDDEIFIVDRLKEVIKFKGFQVPPAELESLLINHHSIADAAVVPQNDEVAGEVPVAFVVR 513

Query: 491 LKNSEATEDEIKQFISKQVVFYKKINRVFFIDAIPKSPSGKILRKELRAKL 539
              ++ TE+++K++++KQVVFYK++++VFF+ +IPKSPSGKILRK+L+AKL
Sbjct: 514 SNGNDITEEDVKEYVAKQVVFYKRLHKVFFVASIPKSPSGKILRKDLKAKL 560

BLAST of MELO3C024886.2.1 vs. TAIR10
Match: AT3G21230.1 (4-coumarate:CoA ligase 5)

HSP 1 Score: 673.3 bits (1736), Expect = 1.2e-193
Identity = 331/545 (60.73%), Postives = 418/545 (76.70%), Query Frame = 0

Query: 4   ETVENDVIFRSKLPDIYIPNHLPLHSYCLQE---NAAKIGHRTCLINGVTGESFTFNDVD 63
           E   +D IFRSKLPDI+IPNHLPL  Y  Q    +       TC+I+G TG   T+ DV 
Sbjct: 21  EEPSHDFIFRSKLPDIFIPNHLPLTDYVFQRFSGDGDGDSSTTCIIDGATGRILTYADVQ 80

Query: 64  LTARKVASGLNKLGITKRDVIMLLLPNSPEFVFAFLGASHLGAIMTAANPFFTAAEIAKQ 123
              R++A+G+++LGI   DV+MLLLPNSPEF  +FL  ++LGA+ T ANPF+T  EIAKQ
Sbjct: 81  TNMRRIAAGIHRLGIRHGDVVMLLLPNSPEFALSFLAVAYLGAVSTTANPFYTQPEIAKQ 140

Query: 124 AKGSKAKLIITQSSYYEKLKEITEELPEVKIM-------TVDSPHDGCLWFGDLIKADER 183
           AK S AK+IIT+    +KL  +  +   +  +        V S  DGC+ F +L +ADE 
Sbjct: 141 AKASAAKMIITKKCLVDKLTNLKNDGVLIVCLDDDGDNGVVSSSDDGCVSFTELTQADET 200

Query: 184 EIPRVEIDPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGNEDVILC 243
           E+ + +I P+D VA+PYSSGTTGLPKGVM+THK LVTS+AQ+VDGENPNL +   DVILC
Sbjct: 201 ELLKPKISPEDTVAMPYSSGTTGLPKGVMITHKGLVTSIAQKVDGENPNLNFTANDVILC 260

Query: 244 VLPLFHIYSLNSVLLCGLRAGSTILIMPKFEIGLLLQLVEKYGVTVAPIVPPIVLAIAKS 303
            LP+FHIY+L++++L  +R G+ +LI+P+FE+ L+++L+++Y VTV P+ PP+VLA  KS
Sbjct: 261 FLPMFHIYALDALMLSAMRTGAALLIVPRFELNLVMELIQRYKVTVVPVAPPVVLAFIKS 320

Query: 304 PELEKYDLSSIRVIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKE 363
           PE E+YDLSS+R++ SG A L KELED VR KFP A+ GQGYGMTE+G V    LAFAK 
Sbjct: 321 PETERYDLSSVRIMLSGAATLKKELEDAVRLKFPNAIFGQGYGMTESGTV-AKSLAFAKN 380

Query: 364 PFPMKPGACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATID 423
           PF  K GACGTV+RNAEMK+VDTETG SLPRN  GEIC+RG Q+MKGYLN+PEATA TID
Sbjct: 381 PFKTKSGACGTVIRNAEMKVVDTETGISLPRNKSGEICVRGHQLMKGYLNDPEATARTID 440

Query: 424 KDGWLHTGDIGFIDEDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMK 483
           KDGWLHTGDIGF+D+DDE+FIVDRLKELIK+KG+QVAPAELEALL++HP I DAAVV MK
Sbjct: 441 KDGWLHTGDIGFVDDDDEIFIVDRLKELIKFKGYQVAPAELEALLISHPSIDDAAVVAMK 500

Query: 484 DEQAGEVPVAFVVKLKNSEATEDEIKQFISKQVVFYKKINRVFFIDAIPKSPSGKILRKE 539
           DE A EVPVAFV + + S+ TED++K +++KQVV YK+I  VFFI+ IPK+ SGKILRK+
Sbjct: 501 DEVADEVPVAFVARSQGSQLTEDDVKSYVNKQVVHYKRIKMVFFIEVIPKAVSGKILRKD 560

BLAST of MELO3C024886.2.1 vs. TAIR10
Match: AT4G05160.1 (AMP-dependent synthetase and ligase family protein)

HSP 1 Score: 423.7 bits (1088), Expect = 1.7e-118
Identity = 237/543 (43.65%), Postives = 341/543 (62.80%), Query Frame = 0

Query: 9   DVIFRSKLPDIYIPN--HLPLHSYCLQENAAKIGHRTCLINGVTGESFTFNDVDLTARKV 68
           D I+RS  P + +P   +  L S+ L  N++    +  + +  TG+S TF+ +     ++
Sbjct: 9   DGIYRSLRPTLVLPKDPNTSLVSF-LFRNSSSYPSKLAIADSDTGDSLTFSQLKSAVARL 68

Query: 69  ASGLNKLGITKRDVIMLLLPNSPEFVFAFLGASHLGAIMTAANPFFTAAEIAKQAKGSKA 128
           A G ++LGI K DV+++  PNS +F   FL  + +G + T ANP +T  E++KQ K S  
Sbjct: 69  AHGFHRLGIRKNDVVLIFAPNSYQFPLCFLAVTAIGGVFTTANPLYTVNEVSKQIKDSNP 128

Query: 129 KLIITQSSYYEKLKEITEELPEVKIMTVDS------PHDGCLWFGDLIKADE--REIPRV 188
           K+II+ +  ++K+K    +LP V + + D+       +   L F ++++  E   E P V
Sbjct: 129 KIIISVNQLFDKIKGF--DLPVVLLGSKDTVEIPPGSNSKILSFDNVMELSEPVSEYPFV 188

Query: 189 EIDPDDVVALPYSSGTTGLPKGVMLTH-----KSLVTSVAQQVDGENPNLYYGNEDVILC 248
           EI   D  AL YSSGTTG  KGV LTH      SL+ ++ Q + GE    Y+G   V LC
Sbjct: 189 EIKQSDTAALLYSSGTTGTSKGVELTHGNFIAASLMVTMDQDLMGE----YHG---VFLC 248

Query: 249 VLPLFHIYSLNSVLLCGLRAGSTILIMPKFEIGLLLQLVEKYGVTVAPIVPPIVLAIAKS 308
            LP+FH++ L  +    L+ G+ ++ M +FE+ L+L+ +EK+ VT   +VPP+ LA++K 
Sbjct: 249 FLPMFHVFGLAVITYSQLQRGNALVSMARFELELVLKNIEKFRVTHLWVVPPVFLALSKQ 308

Query: 309 PELEKYDLSSIRVIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKE 368
             ++K+DLSS++ I SG APLGK+L +      P  +L QGYGMTE     T G+   ++
Sbjct: 309 SIVKKFDLSSLKYIGSGAAPLGKDLMEECGRNIPNVLLMQGYGMTE-----TCGIVSVED 368

Query: 369 PFPMK--PGACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAAT 428
           P   K   G+ G +    E +IV  ETG S P N  GEI +RG  +MKGYLNNP+AT  T
Sbjct: 369 PRLGKRNSGSAGMLAPGVEAQIVSVETGKSQPPNQQGEIWVRGPNMMKGYLNNPQATKET 428

Query: 429 IDKDGWLHTGDIGFIDEDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVP 488
           IDK  W+HTGD+G+ +ED  L++VDR+KELIKYKGFQVAPAELE LL++HP I DA V+P
Sbjct: 429 IDKKSWVHTGDLGYFNEDGNLYVVDRIKELIKYKGFQVAPAELEGLLVSHPDILDAVVIP 488

Query: 489 MKDEQAGEVPVAFVVKLKNSEATEDEIKQFISKQVVFYKKINRVFFIDAIPKSPSGKILR 535
             DE+AGEVP+AFVV+  NS  TE +I++FI+KQV  YK++ RV FI  +PKS +GKILR
Sbjct: 489 FPDEEAGEVPIAFVVRSPNSSITEQDIQKFIAKQVAPYKRLRRVSFISLVPKSAAGKILR 536

BLAST of MELO3C024886.2.1 vs. Swiss-Prot
Match: sp|O24146|4CL2_TOBAC (4-coumarate--CoA ligase 2 OS=Nicotiana tabacum OX=4097 GN=4CL2 PE=1 SV=1)

HSP 1 Score: 803.9 bits (2075), Expect = 1.1e-231
Identity = 402/541 (74.31%), Postives = 459/541 (84.84%), Query Frame = 0

Query: 4   ETVENDVIFRSKLPDIYIPNHLPLHSYCLQENAAKIGHRTCLINGVTGESFTFNDVDLTA 63
           +T + D+IFRSKLPDIYIPNHLPLHSYC  EN ++   R CLING   + +T+ DV+L +
Sbjct: 4   DTKQVDIIFRSKLPDIYIPNHLPLHSYCF-ENISEFSSRPCLINGANKQIYTYADVELNS 63

Query: 64  RKVASGLNKLGITKRDVIMLLLPNSPEFVFAFLGASHLGAIMTAANPFFTAAEIAKQAKG 123
           RKVA+GL+K GI  +D IM+LLPNSPEFVFAF+GAS+LGAI T ANP FT AE+ KQAK 
Sbjct: 64  RKVAAGLHKQGIQPKDTIMILLPNSPEFVFAFIGASYLGAISTMANPLFTPAEVVKQAKA 123

Query: 124 SKAKLIITQSSYYEKLKEITEELPEVKIMTVDSPHDGCLWFGDLIKADEREIPRVEIDPD 183
           S AK+I+TQ+ +  K+K+   E  +VKI+ +DS  +GCL F  L +A+E +IP VEI PD
Sbjct: 124 SSAKIIVTQACHVNKVKDYAFE-NDVKIICIDSAPEGCLHFSVLTQANEHDIPEVEIQPD 183

Query: 184 DVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGNEDVILCVLPLFHIYSL 243
           DVVALPYSSGTTGLPKGVMLTHK LVTSVAQQVDGENPNLY  +EDV+LCVLPLFHIYSL
Sbjct: 184 DVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYIHSEDVMLCVLPLFHIYSL 243

Query: 244 NSVLLCGLRAGSTILIMPKFEIGLLLQLVEKYGVTVAPIVPPIVLAIAKSPELEKYDLSS 303
           NSVLLCGLR G+ ILIM KF+I   L+L+++Y VT+ P VPPIVLAIAKSP ++ YDLSS
Sbjct: 244 NSVLLCGLRVGAAILIMQKFDIVSFLELIQRYKVTIGPFVPPIVLAIAKSPMVDDYDLSS 303

Query: 304 IRVIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPGACG 363
           +R + SG APLGKELEDTVRAKFP A LGQGYGMTEAGPVL M LAFAKEPF +K GACG
Sbjct: 304 VRTVMSGAAPLGKELEDTVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFEIKSGACG 363

Query: 364 TVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHTGDI 423
           TVVRNAEMKIVD +TG+SLPRN  GEICIRGDQIMKGYLN+PEATA TIDK+GWL+TGDI
Sbjct: 364 TVVRNAEMKIVDPKTGNSLPRNQSGEICIRGDQIMKGYLNDPEATARTIDKEGWLYTGDI 423

Query: 424 GFIDEDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVPVA 483
           G+ID+DDELFIVDRLKELIKYKGFQVAPAELEALLL HP ISDAAVVPMKDEQAGEVPVA
Sbjct: 424 GYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLNHPNISDAAVVPMKDEQAGEVPVA 483

Query: 484 FVVKLKNSEATEDEIKQFISKQVVFYKKINRVFFIDAIPKSPSGKILRKELRAKLAAAFP 543
           FVV+   S  TEDE+K FISKQV+FYK+I RVFF+DAIPKSPSGKILRK+LRAKLAA  P
Sbjct: 484 FVVRSNGSTITEDEVKDFISKQVIFYKRIKRVFFVDAIPKSPSGKILRKDLRAKLAAGLP 542

Query: 544 N 545
           N
Sbjct: 544 N 542

BLAST of MELO3C024886.2.1 vs. Swiss-Prot
Match: sp|O24145|4CL1_TOBAC (4-coumarate--CoA ligase 1 OS=Nicotiana tabacum OX=4097 GN=4CL1 PE=2 SV=1)

HSP 1 Score: 799.7 bits (2064), Expect = 2.1e-230
Identity = 395/536 (73.69%), Postives = 452/536 (84.33%), Query Frame = 0

Query: 9   DVIFRSKLPDIYIPNHLPLHSYCLQENAAKIGHRTCLINGVTGESFTFNDVDLTARKVAS 68
           D+IFRSKLPDIYIP HLPLHSYC  EN ++   R CLING   + +T+ +V+LT RKVA 
Sbjct: 14  DLIFRSKLPDIYIPKHLPLHSYCF-ENISEFSSRPCLINGANDQIYTYAEVELTCRKVAV 73

Query: 69  GLNKLGITKRDVIMLLLPNSPEFVFAFLGASHLGAIMTAANPFFTAAEIAKQAKGSKAKL 128
           GLNKLGI ++D IM+LLPNSPEFVFAF+GAS+LGAI T ANP FT AE+ KQAK S AK+
Sbjct: 74  GLNKLGIQQKDTIMILLPNSPEFVFAFMGASYLGAISTMANPLFTPAEVVKQAKASSAKI 133

Query: 129 IITQSSYYEKLKEITEELPEVKIMTVDSPHDGCLWFGDLIKADEREIPRVEIDPDDVVAL 188
           IITQS +  K+K+   E  +VK++ +DS  +GCL F +L ++DE EIP V+I PDDVVAL
Sbjct: 134 IITQSCFVGKVKDYASE-NDVKVICIDSAPEGCLHFSELTQSDEHEIPEVKIQPDDVVAL 193

Query: 189 PYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGNEDVILCVLPLFHIYSLNSVLL 248
           PYSSGTTGLPKGVMLTHK LVTSVAQQVDGEN NLY  +EDV++CVLPLFHIYSLNS+LL
Sbjct: 194 PYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSEDVLMCVLPLFHIYSLNSILL 253

Query: 249 CGLRAGSTILIMPKFEIGLLLQLVEKYGVTVAPIVPPIVLAIAKSPELEKYDLSSIRVIK 308
           CGLR G+ ILIM KF+I   L+L++KY V++ P VPPIVLAIAKSP ++ YDLSS+R + 
Sbjct: 254 CGLRVGAAILIMQKFDIAPFLELIQKYKVSIGPFVPPIVLAIAKSPIVDSYDLSSVRTVM 313

Query: 309 SGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPGACGTVVRN 368
           SG APLGKELED VR KFP A LGQGYGMTEAGPVL M LAFAKEPF +K GACGTVVRN
Sbjct: 314 SGAAPLGKELEDAVRTKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGACGTVVRN 373

Query: 369 AEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHTGDIGFIDE 428
           AEMKIVD +TG SLPRN PGEICIRGDQIMKGYLN+PEAT  TIDK+GWLHTGDIGFIDE
Sbjct: 374 AEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATTRTIDKEGWLHTGDIGFIDE 433

Query: 429 DDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVPVAFVVKL 488
           DDELFIVDRLKELIKYKGFQVAPAE+EALLL HP ISDAAVVPMKDEQAGEVPVAFVV+ 
Sbjct: 434 DDELFIVDRLKELIKYKGFQVAPAEIEALLLNHPNISDAAVVPMKDEQAGEVPVAFVVRS 493

Query: 489 KNSEATEDEIKQFISKQVVFYKKINRVFFIDAIPKSPSGKILRKELRAKLAAAFPN 545
             S  TEDE+K FISKQV+FYK++ RVFF++ +PKSPSGKILRK+LRA+LAA  PN
Sbjct: 494 NGSAITEDEVKDFISKQVIFYKRVKRVFFVETVPKSPSGKILRKDLRARLAAGVPN 547

BLAST of MELO3C024886.2.1 vs. Swiss-Prot
Match: sp|O24540|4CL_VANPL (4-coumarate--CoA ligase OS=Vanilla planifolia OX=51239 GN=4CL PE=3 SV=1)

HSP 1 Score: 796.6 bits (2056), Expect = 1.8e-229
Identity = 408/546 (74.73%), Postives = 457/546 (83.70%), Query Frame = 0

Query: 1   MGIETVENDVIFRSKLPDIYIPNHLPLHSYCLQENAAKIGHRTCLINGVTGESFTFNDVD 60
           + IE  + D+IFRSKLPDIYIP +LPLHSYC  EN +K   R CLING T E FT+ DV+
Sbjct: 5   VAIEEQKKDIIFRSKLPDIYIPKNLPLHSYCF-ENISKFSSRPCLINGATDEIFTYADVE 64

Query: 61  LTARKVASGLNKLGITKRDVIMLLLPNSPEFVFAFLGASHLGAIMTAANPFFTAAEIAKQ 120
           L +R+V SGL+KLGI + D IM+LLPNSPEFVFAFLGAS +G+I T ANPFFT+ E+ KQ
Sbjct: 65  LISRRVGSGLSKLGIKQGDTIMILLPNSPEFVFAFLGASFIGSISTMANPFFTSTEVIKQ 124

Query: 121 AKGSKAKLIITQSSYYEKLKEITEELPEVKIMTVDSPHD-----GCLWFGDLIKADEREI 180
           AK S AKLIITQ  Y +K+K+   E   VKI+++D+          L F +L  ADE E+
Sbjct: 125 AKASNAKLIITQGCYVDKVKDYACE-NGVKIISIDTTTTADDAANILHFSELTGADENEM 184

Query: 181 PRVEIDPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGNEDVILCVL 240
           P+VEI PD VVALPYSSGTTGLPKGVMLTHK LVTSVAQQVDGENPNLY  ++DV+LCVL
Sbjct: 185 PKVEISPDGVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYMHSDDVLLCVL 244

Query: 241 PLFHIYSLNSVLLCGLRAGSTILIMPKFEIGLLLQLVEKYGVTVAPIVPPIVLAIAKSPE 300
           PLFHIYSLNSVLLCGLRAGS ILIM KFEI   L+L++KY VT+ P VPPIVLAIAKS  
Sbjct: 245 PLFHIYSLNSVLLCGLRAGSGILIMQKFEIVPFLELIQKYKVTIGPFVPPIVLAIAKSTV 304

Query: 301 LEKYDLSSIRVIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPF 360
           ++ YDLSS+R + SG APLGKELED VRAKFP A LGQGYGMTEAGPVL M LAFAKEPF
Sbjct: 305 VDNYDLSSVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPF 364

Query: 361 PMKPGACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKD 420
            +K GACGTVVRNAEMKIVD ETGSSLPRN PGEICIRGDQIMKGYLN+PEATA TIDK+
Sbjct: 365 DIKSGACGTVVRNAEMKIVDPETGSSLPRNHPGEICIRGDQIMKGYLNDPEATARTIDKE 424

Query: 421 GWLHTGDIGFIDEDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDE 480
           GWLHTGDIG+ID+DDELFIVDRLKELIKYKGFQVAPAELEALLLTHP ISDAAVVPMKDE
Sbjct: 425 GWLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPCISDAAVVPMKDE 484

Query: 481 QAGEVPVAFVVKLKNSEATEDEIKQFISKQVVFYKKINRVFFIDAIPKSPSGKILRKELR 540
            AGEVPVAFVVK      TEDEIKQFISKQV+FYK+INRVFF++AIPK+PSGKILRK+LR
Sbjct: 485 AAGEVPVAFVVKSNGHNITEDEIKQFISKQVIFYKRINRVFFVEAIPKAPSGKILRKDLR 544

Query: 541 AKLAAA 542
           A+LAAA
Sbjct: 545 ARLAAA 548

BLAST of MELO3C024886.2.1 vs. Swiss-Prot
Match: sp|P14913|4CL2_PETCR (4-coumarate--CoA ligase 1 OS=Petroselinum crispum OX=4043 GN=4CL2 PE=2 SV=1)

HSP 1 Score: 791.2 bits (2042), Expect = 7.4e-228
Identity = 394/534 (73.78%), Postives = 455/534 (85.21%), Query Frame = 0

Query: 7   ENDVIFRSKLPDIYIPNHLPLHSYCLQENAAKIGHRTCLINGVTGESFTFNDVDLTARKV 66
           + D+IFRSKLPDIYIP HLPLH+YC  EN +K+G ++CLING TGE+FT++ V+L +RKV
Sbjct: 8   KEDLIFRSKLPDIYIPKHLPLHTYCF-ENISKVGDKSCLINGATGETFTYSQVELLSRKV 67

Query: 67  ASGLNKLGITKRDVIMLLLPNSPEFVFAFLGASHLGAIMTAANPFFTAAEIAKQAKGSKA 126
           ASGLNKLGI + D IMLLLPNSPE+ FAFLGAS+ GAI T ANPFFT+AE+ KQ K S A
Sbjct: 68  ASGLNKLGIQQGDTIMLLLPNSPEYFFAFLGASYRGAISTMANPFFTSAEVIKQLKASLA 127

Query: 127 KLIITQSSYYEKLKEITEELPEVKIMTVDSPHDGCLWFGDLIKADEREIPRVEIDPDDVV 186
           KLIITQ+ Y +K+K+   E   ++I+ +D     CL F  L++ADE E+P V ID DDVV
Sbjct: 128 KLIITQACYVDKVKDYAAE-KNIQIICIDDAPQDCLHFSKLMEADESEMPEVVIDSDDVV 187

Query: 187 ALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGNEDVILCVLPLFHIYSLNSV 246
           ALPYSSGTTGLPKGVMLTHK LVTSVAQQVDG+NPNLY  +EDV++C+LPLFHIYSLN+V
Sbjct: 188 ALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYMHSEDVMICILPLFHIYSLNAV 247

Query: 247 LLCGLRAGSTILIMPKFEIGLLLQLVEKYGVTVAPIVPPIVLAIAKSPELEKYDLSSIRV 306
           L CGLRAG TILIM KF+I   L+L++KY VT+ P VPPIVLAIAKSP ++KYDLSS+R 
Sbjct: 248 LCCGLRAGVTILIMQKFDIVPFLELIQKYKVTIGPFVPPIVLAIAKSPVVDKYDLSSVRT 307

Query: 307 IKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPGACGTVV 366
           + SG APLGKELED VRAKFP A LGQGYGMTEAGPVL M LAFAKEP+ +K GACGTVV
Sbjct: 308 VMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPYEIKSGACGTVV 367

Query: 367 RNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHTGDIGFI 426
           RNAEMKIVD ET +SLPRN  GEICIRGDQIMKGYLN+PE+T  TID++GWLHTGDIGFI
Sbjct: 368 RNAEMKIVDPETNASLPRNQRGEICIRGDQIMKGYLNDPESTRTTIDEEGWLHTGDIGFI 427

Query: 427 DEDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVPVAFVV 486
           D+DDELFIVDRLKE+IKYKGFQVAPAELEALLLTHP ISDAAVVPM DE+AGEVPVAFVV
Sbjct: 428 DDDDELFIVDRLKEIIKYKGFQVAPAELEALLLTHPTISDAAVVPMIDEKAGEVPVAFVV 487

Query: 487 KLKNSEATEDEIKQFISKQVVFYKKINRVFFIDAIPKSPSGKILRKELRAKLAA 541
           +      TE+EIKQF+SKQVVFYK+I RVFF+DAIPKSPSGKILRK+LRAK+A+
Sbjct: 488 RTNGFTTTEEEIKQFVSKQVVFYKRIFRVFFVDAIPKSPSGKILRKDLRAKIAS 539

BLAST of MELO3C024886.2.1 vs. Swiss-Prot
Match: sp|P14912|4CL1_PETCR (4-coumarate--CoA ligase 1 OS=Petroselinum crispum OX=4043 GN=4CL1 PE=2 SV=1)

HSP 1 Score: 789.3 bits (2037), Expect = 2.8e-227
Identity = 392/534 (73.41%), Postives = 456/534 (85.39%), Query Frame = 0

Query: 7   ENDVIFRSKLPDIYIPNHLPLHSYCLQENAAKIGHRTCLINGVTGESFTFNDVDLTARKV 66
           + D+IFRSKLPDIYIP HLPLH+YC  EN +K+G ++CLING TGE+FT++ V+L +RKV
Sbjct: 8   KEDLIFRSKLPDIYIPKHLPLHTYCF-ENISKVGDKSCLINGATGETFTYSQVELLSRKV 67

Query: 67  ASGLNKLGITKRDVIMLLLPNSPEFVFAFLGASHLGAIMTAANPFFTAAEIAKQAKGSKA 126
           ASGLNKLGI + D IMLLLPNSPE+ FAFLGAS+ GAI T ANPFFT+AE+ KQ K S+A
Sbjct: 68  ASGLNKLGIQQGDTIMLLLPNSPEYFFAFLGASYRGAISTMANPFFTSAEVIKQLKASQA 127

Query: 127 KLIITQSSYYEKLKEITEELPEVKIMTVDSPHDGCLWFGDLIKADEREIPRVEIDPDDVV 186
           KLIITQ+ Y +K+K+   E   ++I+ +D     CL F  L++ADE E+P V I+ DDVV
Sbjct: 128 KLIITQACYVDKVKDYAAE-KNIQIICIDDAPQDCLHFSKLMEADESEMPEVVINSDDVV 187

Query: 187 ALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGNEDVILCVLPLFHIYSLNSV 246
           ALPYSSGTTGLPKGVMLTHK LVTSVAQQVDG+NPNLY  +EDV++C+LPLFHIYSLN+V
Sbjct: 188 ALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYMHSEDVMICILPLFHIYSLNAV 247

Query: 247 LLCGLRAGSTILIMPKFEIGLLLQLVEKYGVTVAPIVPPIVLAIAKSPELEKYDLSSIRV 306
           L CGLRAG TILIM KF+I   L+L++KY VT+ P VPPIVLAIAKSP ++KYDLSS+R 
Sbjct: 248 LCCGLRAGVTILIMQKFDIVPFLELIQKYKVTIGPFVPPIVLAIAKSPVVDKYDLSSVRT 307

Query: 307 IKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPGACGTVV 366
           + SG APLGKELED VRAKFP A LGQGYGMTEAGPVL M LAFAKEP+ +K GACGTVV
Sbjct: 308 VMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPYEIKSGACGTVV 367

Query: 367 RNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHTGDIGFI 426
           RNAEMKIVD ET +SLPRN  GEICIRGDQIMKGYLN+PE+T  TID++GWLHTGDIGFI
Sbjct: 368 RNAEMKIVDPETNASLPRNQRGEICIRGDQIMKGYLNDPESTRTTIDEEGWLHTGDIGFI 427

Query: 427 DEDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVPVAFVV 486
           D+DDELFIVDRLKE+IKYKGFQVAPAELEALLLTHP ISDAAVVPM DE+AGEVPVAFVV
Sbjct: 428 DDDDELFIVDRLKEIIKYKGFQVAPAELEALLLTHPTISDAAVVPMIDEKAGEVPVAFVV 487

Query: 487 KLKNSEATEDEIKQFISKQVVFYKKINRVFFIDAIPKSPSGKILRKELRAKLAA 541
           +      TE+EIKQF+SKQVVFYK+I RVFF+DAIPKSPSGKILRK+LRA++A+
Sbjct: 488 RTNGFTTTEEEIKQFVSKQVVFYKRIFRVFFVDAIPKSPSGKILRKDLRARIAS 539

BLAST of MELO3C024886.2.1 vs. TrEMBL
Match: tr|A0A1S3CJP5|A0A1S3CJP5_CUCME (4-coumarate--CoA ligase 1-like OS=Cucumis melo OX=3656 GN=LOC103501216 PE=4 SV=1)

HSP 1 Score: 1004.6 bits (2596), Expect = 8.6e-290
Identity = 510/519 (98.27%), Postives = 513/519 (98.84%), Query Frame = 0

Query: 1   MGIETVENDVIFRSKLPDIYIPNHLPLHSYCLQENAAKIGHRTCLINGVTGESFTFNDVD 60
           MGIETVENDVIFRSKLPDIYIPNHLPLHSYCLQENAAKIGHRTCLINGVTGESFTFNDVD
Sbjct: 1   MGIETVENDVIFRSKLPDIYIPNHLPLHSYCLQENAAKIGHRTCLINGVTGESFTFNDVD 60

Query: 61  LTARKVASGLNKLGITKRDVIMLLLPNSPEFVFAFLGASHLGAIMTAANPFFTAAEIAKQ 120
           LTARKVASGLNKLGITKRDVIMLLLPNSPEFVFAFLGASHLGAIMTAANPFFTAAEIAKQ
Sbjct: 61  LTARKVASGLNKLGITKRDVIMLLLPNSPEFVFAFLGASHLGAIMTAANPFFTAAEIAKQ 120

Query: 121 AKGSKAKLIITQSSYYEKLKEITEELPEVKIMTVDSPHDGCLWFGDLIKADEREIPRVEI 180
           AKGSKAKLIITQSSYYEKLKEITEELPEVKIMTVDSPHDGCLWFGDLIKADEREIPRVEI
Sbjct: 121 AKGSKAKLIITQSSYYEKLKEITEELPEVKIMTVDSPHDGCLWFGDLIKADEREIPRVEI 180

Query: 181 DPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGNEDVILCVLPLFHI 240
           DPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGNEDVILCVLPLFHI
Sbjct: 181 DPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGNEDVILCVLPLFHI 240

Query: 241 YSLNSVLLCGLRAGSTILIMPKFEIGLLLQLVEKYGVTVAPIVPPIVLAIAKSPELEKYD 300
           YSLNSVLLCGLRAGSTILIMPKFEIGLLLQLVEKYGVTVAPIVPPIVLAIAKSPELEKYD
Sbjct: 241 YSLNSVLLCGLRAGSTILIMPKFEIGLLLQLVEKYGVTVAPIVPPIVLAIAKSPELEKYD 300

Query: 301 LSSIRVIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360
           LSSIRVIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG
Sbjct: 301 LSSIRVIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360

Query: 361 ACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420
           ACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT
Sbjct: 361 ACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420

Query: 421 GDIGFIDEDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480
           GDIGFIDEDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV
Sbjct: 421 GDIGFIDEDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480

Query: 481 PVAFVVKLKNSEATEDEIKQFISKQV-VFY--KKINRVF 517
           PVAFVVKLKNSEATEDEIKQFISKQV VF+    +N +F
Sbjct: 481 PVAFVVKLKNSEATEDEIKQFISKQVSVFFYLTNLNSIF 519

BLAST of MELO3C024886.2.1 vs. TrEMBL
Match: tr|A0A0A0LF00|A0A0A0LF00_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G710200 PE=4 SV=1)

HSP 1 Score: 890.2 bits (2299), Expect = 2.4e-255
Identity = 448/465 (96.34%), Postives = 461/465 (99.14%), Query Frame = 0

Query: 82  MLLLPNSPEFVFAFLGASHLGAIMTAANPFFTAAEIAKQAKGSKAKLIITQSSYYEKLKE 141
           MLLLPNSPEFVFAFLGAS+LGAIMTAANPFFTAAEI KQAKGSK+KLIITQSSYYEK+KE
Sbjct: 1   MLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIGKQAKGSKSKLIITQSSYYEKVKE 60

Query: 142 ITEELPEVKIMTVDSPHDGCLWFGDLIKADEREIPRVEIDPDDVVALPYSSGTTGLPKGV 201
           ITEELPEVKIMTVDSP DGCLWFGDLIKADERE+PRV+IDP+DVVALPYSSGTTGLPKGV
Sbjct: 61  ITEELPEVKIMTVDSPPDGCLWFGDLIKADEREVPRVDIDPEDVVALPYSSGTTGLPKGV 120

Query: 202 MLTHKSLVTSVAQQVDGENPNLYYGNEDVILCVLPLFHIYSLNSVLLCGLRAGSTILIMP 261
           MLTHKSLVTSVAQQVDGENPNLYYG+EDVILCVLPLFHIYSLNSVLLCGLRAGSTILIMP
Sbjct: 121 MLTHKSLVTSVAQQVDGENPNLYYGHEDVILCVLPLFHIYSLNSVLLCGLRAGSTILIMP 180

Query: 262 KFEIGLLLQLVEKYGVTVAPIVPPIVLAIAKSPELEKYDLSSIRVIKSGGAPLGKELEDT 321
           KFEIGLLLQL EKYGVTVAPIVPPIVLAIAKSPELEKYDLSSIR+IKSGGAPLGKELEDT
Sbjct: 181 KFEIGLLLQLAEKYGVTVAPIVPPIVLAIAKSPELEKYDLSSIRIIKSGGAPLGKELEDT 240

Query: 322 VRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPGACGTVVRNAEMKIVDTETGSS 381
           VRAKFPKAVLGQGYGMTEAGPVLTMGLAFAK+PFPMKPGACGTVVRNAEMKIVDTETGSS
Sbjct: 241 VRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKDPFPMKPGACGTVVRNAEMKIVDTETGSS 300

Query: 382 LPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHTGDIGFIDEDDELFIVDRLKEL 441
           LPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHTGDIGFID+DDE+FIVDRLKEL
Sbjct: 301 LPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHTGDIGFIDDDDEMFIVDRLKEL 360

Query: 442 IKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVPVAFVVKLKNSEATEDEIKQF 501
           IKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVPVAFVVKLKNSEA+EDEIKQF
Sbjct: 361 IKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVPVAFVVKLKNSEASEDEIKQF 420

Query: 502 ISKQVVFYKKINRVFFIDAIPKSPSGKILRKELRAKLAAAFPNSN 547
           ISKQVVFYK+INRVFFIDAIPKSPSGKILRKELRAKLAAAFP SN
Sbjct: 421 ISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAAAFPTSN 465

BLAST of MELO3C024886.2.1 vs. TrEMBL
Match: tr|A0A2K9VP19|A0A2K9VP19_MALDO (4-coumarate:coenzyme A ligase 1 OS=Malus domestica OX=3750 GN=4CL1 PE=2 SV=1)

HSP 1 Score: 845.5 bits (2183), Expect = 6.7e-242
Identity = 421/548 (76.82%), Postives = 480/548 (87.59%), Query Frame = 0

Query: 1   MGIETVENDVIFRSKLPDIYIPNHLPLHSYCLQENAAKIGHRTCLINGVTGESFTFNDVD 60
           M IET+ ND+++RSKLPDI IP HLPLHSYCL  N      + C+I+G TG+ +TF DV+
Sbjct: 1   MAIETIPNDIVYRSKLPDIPIPKHLPLHSYCL-HNKNHSSSKPCIIDGATGDIYTFADVE 60

Query: 61  LTARKVASGLNKLGITKRDVIMLLLPNSPEFVFAFLGASHLGAIMTAANPFFTAAEIAKQ 120
           L AR+VASGLNKLGI + DVIMLLLPNSP F FAFLGAS  GA+ TAANPFFT AEI KQ
Sbjct: 61  LNARRVASGLNKLGIQQGDVIMLLLPNSPAFAFAFLGASFRGAMTTAANPFFTPAEILKQ 120

Query: 121 AKGSKAKLIITQSSYYEKLKEI---TEELPEVKIMTVDSPHD-GCLWFGDLIKADEREIP 180
           AK SKAKLIIT + YY+K+K++   ++++ ++K+M VDSP D  CL F +L++ADE+++P
Sbjct: 121 AKASKAKLIITLACYYDKVKDLSSSSDDVHDIKLMCVDSPPDPSCLHFSELLQADEKDMP 180

Query: 181 RVEIDPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGNEDVILCVLP 240
            V+I PDDVVALPYSSGTTGLPKGVMLTHK LVTSVAQQVDGENPNLYY  +DVILCVLP
Sbjct: 181 EVDISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYYSTDDVILCVLP 240

Query: 241 LFHIYSLNSVLLCGLRAGSTILIMPKFEIGLLLQLVEKYGVTVAPIVPPIVLAIAKSPEL 300
           LFHIYSLNSVLLCGLRAG+ IL+M KFEI  LL L++KY V++APIVPPIVLAIAK P+L
Sbjct: 241 LFHIYSLNSVLLCGLRAGAAILMMNKFEIVSLLGLIDKYKVSIAPIVPPIVLAIAKFPDL 300

Query: 301 EKYDLSSIRVIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFP 360
           +KYDLSSIRV+K GGAPLGKELEDTVRAKFP   LGQGYGMTEAGPVLTM LAFAK+PF 
Sbjct: 301 DKYDLSSIRVLKCGGAPLGKELEDTVRAKFPNVTLGQGYGMTEAGPVLTMSLAFAKQPFE 360

Query: 361 MKPGACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDG 420
           +KPG CGTVVRNAE+KIVD E+G+SLPRN PGEICIRGDQIMKGYLN+PE+T  TIDK+G
Sbjct: 361 VKPGGCGTVVRNAELKIVDPESGASLPRNQPGEICIRGDQIMKGYLNDPESTRTTIDKEG 420

Query: 421 WLHTGDIGFIDEDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQ 480
           WLHTGDIGFID+DDELFIVDRLKELIKYKGFQVAPAELEALL+THP +SDAAVVPMKDE 
Sbjct: 421 WLHTGDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLITHPSVSDAAVVPMKDEA 480

Query: 481 AGEVPVAFVVKLKNSEATEDEIKQFISKQVVFYKKINRVFFIDAIPKSPSGKILRKELRA 540
           AGEVPVAFVV+  NS+ TEDE+KQFISKQVVFYK+INRVFFI+AIPKSPSGKILRK+LRA
Sbjct: 481 AGEVPVAFVVRSNNSQLTEDEVKQFISKQVVFYKRINRVFFIEAIPKSPSGKILRKDLRA 540

Query: 541 KLAAAFPN 545
           KLAA FPN
Sbjct: 541 KLAAGFPN 547

BLAST of MELO3C024886.2.1 vs. TrEMBL
Match: tr|D5LLN7|D5LLN7_SORAU (p-coumarate:CoA ligase 1 OS=Sorbus aucuparia OX=36599 PE=2 SV=1)

HSP 1 Score: 844.3 bits (2180), Expect = 1.5e-241
Identity = 420/548 (76.64%), Postives = 479/548 (87.41%), Query Frame = 0

Query: 1   MGIETVENDVIFRSKLPDIYIPNHLPLHSYCLQENAAKIGHRTCLINGVTGESFTFNDVD 60
           M IET+ ND+++RSKLPDI IP HLPLHSYCL  N      + C+I+G TG+ +TF DV+
Sbjct: 1   MAIETIPNDIVYRSKLPDIPIPKHLPLHSYCL-HNKNHSSSKPCIIDGATGDIYTFADVE 60

Query: 61  LTARKVASGLNKLGITKRDVIMLLLPNSPEFVFAFLGASHLGAIMTAANPFFTAAEIAKQ 120
           L AR+VASGLNKLGI + DVIMLLLPNSP F FAFLGAS  GA+ TAANPFFT AEI KQ
Sbjct: 61  LNARRVASGLNKLGIQQGDVIMLLLPNSPAFAFAFLGASFRGAMTTAANPFFTPAEILKQ 120

Query: 121 AKGSKAKLIITQSSYYEKLKEI---TEELPEVKIMTVDSPHD-GCLWFGDLIKADEREIP 180
           AK SKAKLIIT + YY+K+K++   ++++ ++K+M VDSP D  CL F +L++ADE ++P
Sbjct: 121 AKASKAKLIITLACYYDKVKDLSSSSDDVHDIKLMCVDSPPDPSCLHFSELLQADENDMP 180

Query: 181 RVEIDPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGNEDVILCVLP 240
            V+I PDDVVALPYSSGTTGLPKGVMLTHK LVTSVAQQVDGENPNLYY  +DV+LCVLP
Sbjct: 181 EVDISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYYSTDDVVLCVLP 240

Query: 241 LFHIYSLNSVLLCGLRAGSTILIMPKFEIGLLLQLVEKYGVTVAPIVPPIVLAIAKSPEL 300
           LFHIYSLNSVLLCGLRAG+ IL+M KFEI  LL L++KY V++APIVPPIVLAIAK P+L
Sbjct: 241 LFHIYSLNSVLLCGLRAGAAILMMNKFEIVSLLGLIDKYKVSIAPIVPPIVLAIAKFPDL 300

Query: 301 EKYDLSSIRVIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFP 360
           +KYDLSSIRV+K GGAPLGKELEDTVRAKFP   LGQGYGMTEAGPVLTM LAFAK+PF 
Sbjct: 301 DKYDLSSIRVLKCGGAPLGKELEDTVRAKFPNVTLGQGYGMTEAGPVLTMSLAFAKQPFE 360

Query: 361 MKPGACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDG 420
           +KPG CGTVVRNAE+KIVD E+G+SLPRN PGEICIRGDQIMKGYLN+PE+T  TIDK+G
Sbjct: 361 VKPGGCGTVVRNAELKIVDPESGASLPRNQPGEICIRGDQIMKGYLNDPESTRTTIDKEG 420

Query: 421 WLHTGDIGFIDEDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQ 480
           WLHTGDIGFID+DDELFIVDRLKELIKYKGFQVAPAELEALL+THP +SDAAVVPMKDE 
Sbjct: 421 WLHTGDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLITHPSVSDAAVVPMKDEA 480

Query: 481 AGEVPVAFVVKLKNSEATEDEIKQFISKQVVFYKKINRVFFIDAIPKSPSGKILRKELRA 540
           AGEVPVAFVV+  NS+ TEDE+KQFISKQVVFYK+INRVFFI+AIPKSPSGKILRK+LRA
Sbjct: 481 AGEVPVAFVVRSNNSQLTEDEVKQFISKQVVFYKRINRVFFIEAIPKSPSGKILRKDLRA 540

Query: 541 KLAAAFPN 545
           KLAA FPN
Sbjct: 541 KLAAGFPN 547

BLAST of MELO3C024886.2.1 vs. TrEMBL
Match: tr|A0A2N9FA69|A0A2N9FA69_FAGSY (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS11546 PE=4 SV=1)

HSP 1 Score: 842.8 bits (2176), Expect = 4.3e-241
Identity = 423/542 (78.04%), Postives = 471/542 (86.90%), Query Frame = 0

Query: 1   MGIETVENDVIFRSKLPDIYIPNHLPLHSYCLQENAAKIGHRTCLINGVTGESFTFNDVD 60
           M ++T + + IFRSKLPDIYIP HLPLHSYC  EN + +G R CLING TGE  T+ DV+
Sbjct: 1   MALQTKQEEFIFRSKLPDIYIPKHLPLHSYCF-ENISNVGSRPCLINGSTGEVHTYYDVE 60

Query: 61  LTARKVASGLNKLGITKRDVIMLLLPNSPEFVFAFLGASHLGAIMTAANPFFTAAEIAKQ 120
           L ARKVASGLN+LGI K DV++LLLPN+PEFVF FLGASH+GAI TAANPFFT AEIAKQ
Sbjct: 61  LKARKVASGLNQLGIGKADVVLLLLPNTPEFVFVFLGASHIGAITTAANPFFTPAEIAKQ 120

Query: 121 AKGSKAKLIITQSSYYEKLKEITEELPEVKIMTVDSPHDGCLWFGDLIKADEREIPRVEI 180
           AKGS AKLIITQ+SYY+K+K++  E   VKI+ +DSP   CL F +L KADE +IP+V I
Sbjct: 121 AKGSNAKLIITQASYYDKVKDLASENNGVKIICIDSPPQDCLHFSELTKADEHDIPQVNI 180

Query: 181 DPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGNEDVILCVLPLFHI 240
            PDDVVALPYSSGTTGLPKGVMLTHK LVTSVAQQVDGENPNLY+ +EDVILCVLPLFHI
Sbjct: 181 SPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSEDVILCVLPLFHI 240

Query: 241 YSLNSVLLCGLRAGSTILIMPKFEIGLLLQLVEKYGVTVAPIVPPIVLAIAKSPELEKYD 300
           YSLNSVLLCGLR G+ ILIM KFEI  LL+L++K+ V+V PIVPPIVLAI+KSP+L KYD
Sbjct: 241 YSLNSVLLCGLRVGAAILIMQKFEIVSLLELIQKHKVSVMPIVPPIVLAISKSPDLHKYD 300

Query: 301 LSSIRVIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360
           LSSIR++KSGGAPLGKELE+TV AKFP A  GQGYGMTEAGPVL M LAFAK PF +K G
Sbjct: 301 LSSIRILKSGGAPLGKELEETVGAKFPNAKFGQGYGMTEAGPVLAMCLAFAKTPFDVKSG 360

Query: 361 ACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420
           ACGTVVRNAEMKIVD ETG+SLPRN PGEICIRGDQIMKGY+N+P ATAATIDK+GWLHT
Sbjct: 361 ACGTVVRNAEMKIVDPETGASLPRNKPGEICIRGDQIMKGYINDPNATAATIDKEGWLHT 420

Query: 421 GDIGFIDEDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480
           GDIG ID DDELFIVDRLKELIKYKGFQVAPAELEALLLTHP ISDAAVV MKD+QAGEV
Sbjct: 421 GDIGLIDVDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPNISDAAVVSMKDDQAGEV 480

Query: 481 PVAFVVKLKNSEATEDEIKQFISKQVVFYKKINRVFFIDAIPKSPSGKILRKELRAKLAA 540
           PVAFVV+   S  TEDE+KQF+SKQVVFYK+INRVFFI+AIPK+PSGKILRK LR KLAA
Sbjct: 481 PVAFVVRSNGSNITEDEVKQFVSKQVVFYKRINRVFFIEAIPKAPSGKILRKNLREKLAA 540

Query: 541 AF 543
            F
Sbjct: 541 DF 541

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_004137646.21.6e-30396.34PREDICTED: 4-coumarate--CoA ligase 1 [Cucumis sativus][more]
XP_008462955.11.3e-28998.27PREDICTED: 4-coumarate--CoA ligase 1-like [Cucumis melo][more]
XP_022935389.19.0e-28389.744-coumarate--CoA ligase 1-like [Cucurbita moschata][more]
XP_023526871.16.5e-28189.194-coumarate--CoA ligase 1-like isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_022983944.13.2e-28089.194-coumarate--CoA ligase 1-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT3G21240.18.3e-22270.614-coumarate:CoA ligase 2[more]
AT1G51680.15.0e-21971.304-coumarate:CoA ligase 1[more]
AT1G65060.11.1e-19764.414-coumarate:CoA ligase 3[more]
AT3G21230.11.2e-19360.734-coumarate:CoA ligase 5[more]
AT4G05160.11.7e-11843.65AMP-dependent synthetase and ligase family protein[more]
Match NameE-valueIdentityDescription
sp|O24146|4CL2_TOBAC1.1e-23174.314-coumarate--CoA ligase 2 OS=Nicotiana tabacum OX=4097 GN=4CL2 PE=1 SV=1[more]
sp|O24145|4CL1_TOBAC2.1e-23073.694-coumarate--CoA ligase 1 OS=Nicotiana tabacum OX=4097 GN=4CL1 PE=2 SV=1[more]
sp|O24540|4CL_VANPL1.8e-22974.734-coumarate--CoA ligase OS=Vanilla planifolia OX=51239 GN=4CL PE=3 SV=1[more]
sp|P14913|4CL2_PETCR7.4e-22873.784-coumarate--CoA ligase 1 OS=Petroselinum crispum OX=4043 GN=4CL2 PE=2 SV=1[more]
sp|P14912|4CL1_PETCR2.8e-22773.414-coumarate--CoA ligase 1 OS=Petroselinum crispum OX=4043 GN=4CL1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
tr|A0A1S3CJP5|A0A1S3CJP5_CUCME8.6e-29098.274-coumarate--CoA ligase 1-like OS=Cucumis melo OX=3656 GN=LOC103501216 PE=4 SV=1[more]
tr|A0A0A0LF00|A0A0A0LF00_CUCSA2.4e-25596.34Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G710200 PE=4 SV=1[more]
tr|A0A2K9VP19|A0A2K9VP19_MALDO6.7e-24276.824-coumarate:coenzyme A ligase 1 OS=Malus domestica OX=3750 GN=4CL1 PE=2 SV=1[more]
tr|D5LLN7|D5LLN7_SORAU1.5e-24176.64p-coumarate:CoA ligase 1 OS=Sorbus aucuparia OX=36599 PE=2 SV=1[more]
tr|A0A2N9FA69|A0A2N9FA69_FAGSY4.3e-24178.04Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS11546 PE=4 SV=1[more]
The following terms have been associated with this mRNA:
Vocabulary: Biological Process
TermDefinition
GO:0008152metabolic process
Vocabulary: Molecular Function
TermDefinition
GO:0003824catalytic activity
Vocabulary: INTERPRO
TermDefinition
IPR020845AMP-binding_CS
IPR025110AMP-bd_C
IPR000873AMP-dep_Synth/Lig
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008152 metabolic process
cellular_component GO:0005575 cellular_component
molecular_function GO:0003824 catalytic activity
molecular_function GO:0016874 ligase activity

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
MELO3C024886.2MELO3C024886.2gene


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
MELO3C024886.2.1MELO3C024886.2.1-proteinpolypeptide


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
MELO3C024886.2.1.five_prime_UTR.1MELO3C024886.2.1.five_prime_UTR.1five_prime_UTR


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
MELO3C024886.2.1.exon.4MELO3C024886.2.1.exon.4exon
MELO3C024886.2.1.exon.3MELO3C024886.2.1.exon.3exon
MELO3C024886.2.1.exon.2MELO3C024886.2.1.exon.2exon
MELO3C024886.2.1.exon.1MELO3C024886.2.1.exon.1exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
MELO3C024886.2.1.CDS.4MELO3C024886.2.1.CDS.4CDS
MELO3C024886.2.1.CDS.3MELO3C024886.2.1.CDS.3CDS
MELO3C024886.2.1.CDS.2MELO3C024886.2.1.CDS.2CDS
MELO3C024886.2.1.CDS.1MELO3C024886.2.1.CDS.1CDS


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
MELO3C024886.2.1.three_prime_UTR.1MELO3C024886.2.1.three_prime_UTR.1three_prime_UTR


Analysis Name: InterPro Annotations of melon v3.6.1
Date Performed: 2018-09-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3DG3DSA:3.40.50.12780coord: 5..438
e-value: 1.3E-169
score: 566.3
NoneNo IPR availableGENE3DG3DSA:3.30.300.30coord: 439..541
e-value: 2.2E-30
score: 107.3
NoneNo IPR availablePANTHERPTHR24096:SF2074-COUMARATE--COA LIGASE 1-RELATEDcoord: 10..541
NoneNo IPR availablePANTHERPTHR24096FAMILY NOT NAMEDcoord: 10..541
NoneNo IPR availableCDDcd059044CLcoord: 21..534
e-value: 0.0
score: 790.297
NoneNo IPR availableSUPERFAMILYSSF56801Acetyl-CoA synthetase-likecoord: 17..540
IPR000873AMP-dependent synthetase/ligasePFAMPF00501AMP-bindingcoord: 32..444
e-value: 2.3E-112
score: 375.6
IPR025110AMP-binding enzyme, C-terminal domainPFAMPF13193AMP-binding_Ccoord: 453..528
e-value: 2.0E-16
score: 60.6
IPR020845AMP-binding, conserved sitePROSITEPS00455AMP_BINDINGcoord: 188..199