MELO3C024886.2 (gene) Melon (DHL92) v3.6.1

Overview
NameMELO3C024886.2
Typegene
OrganismCucumis melo (Melon (DHL92) v3.6.1)
Description4-coumarate:CoA ligase
Locationchr01 : 11806353 .. 11810755 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTCCATTCTTCGAACTCAGAAGAACAATAAACCTTCAAGAAAAAAGAAAGTTATCTGTTCTTCTTCTTCTTCTTCTTCTTCTTCAATTCGGCGTTGAAATGGGAATTGAAACAGTTGAGAATGACGTTATTTTCCGGTCAAAACTACCGGACATCTACATCCCAAATCATCTTCCTCTGCATTCCTACTGCCTACAAGAAAACGCCGCCAAAATAGGCCACCGAACTTGCTTAATCAACGGCGTTACCGGCGAATCCTTCACCTTCAACGACGTCGATCTCACTGCACGCAAGGTCGCCTCCGGATTAAACAAACTCGGCATTACAAAACGCGACGTAATCATGCTCCTCCTCCCAAATTCACCGGAGTTCGTCTTCGCTTTCCTCGGGGCCTCCCACCTCGGCGCAATCATGACGGCCGCAAACCCTTTCTTCACGGCAGCAGAAATCGCAAAGCAAGCAAAAGGATCAAAAGCAAAACTAATCATAACGCAATCGTCCTACTACGAAAAGTTGAAGGAAATAACAGAGGAATTGCCAGAGGTTAAAATCATGACCGTCGATTCTCCTCACGATGGTTGTTTATGGTTCGGTGATCTAATTAAAGCCGACGAAAGAGAGATTCCGAGGGTGGAAATCGACCCAGATGACGTGGTTGCGCTGCCGTATTCGTCGGGAACGACGGGATTGCCAAAGGGAGTGATGCTTACACATAAAAGTTTGGTTACGAGTGTGGCTCAGCAAGTGGATGGGGAAAATCCAAATCTGTATTATGGAAATGAGGATGTGATTCTGTGTGTTTTGCCGTTGTTTCATATTTATTCTTTGAATTCAGTTTTGCTGTGTGGATTAAGAGCTGGAAGTACCATTTTGATTATGCCCAAATTTGAGATCGGTTTGCTTTTGCAATTGGTGGAGAAATATGGAGTTACCGTGGCGCCCATCGTGCCGCCCATCGTTCTGGCTATTGCAAAGTCGCCGGAGCTAGAGAAGTATGACCTTTCGTCGATCAGAGTTATTAAATCCGGCGGTGCACCGCTTGGGAAGGAGCTTGAAGATACCGTAAGAGCCAAGTTTCCTAAGGCTGTGCTTGGACAGGTATATCATCATGATACAAATTTATAATTTTAAGAACTTTAACAACAACCCTTTGGTTTTTTTGTTTTTAAAAATCAAGTTTCAAAAAACAAAAGAAAAAACTATAGAAAATATTAGATAGGTTTACAAAATAGTAAATATTATAAATATTGAAAATTTTCAATCACGGTTTAAGAATTTTAACTATTATAATGTGGTATCTTAACAGTAAATGTAAGAAAAATATATATTGAATTTGTTATACTCTCAATTTTTAATCACTGGTTTGGTTAAAGAACAATAAGAAACAAATATGTATATATAAAGAACAATTTTCGTTTGGTATTTTCAAAAAACCCGATTTTCATCCTTGTACAATCTACTAATTTTCTTTTAAATATATATATATTTTAAATTGTTTAGGTTTTAGAATGAGTTGCAACAAGATAATAGATGAAAAAATCTAAAGGTTTAATGGGTGGAGATTAGTGGTGTTATTAAATGTAATTAAGATTTTGTTATTAAATGTAATTAAGATTTTGTTATTAAATGTAATTAAGATTTTGTAGATGTGAAGGATAATTAGTCAAAATTCTTGATGTGAAGAAAGTCTTTGAGTGGAGACGAAATGACACCGAAGTCAAAGCAAAAGTCTGCGTGTAATTCTTTAGTTATTAAAGAAAAAATTAATTTAATTCATTTCTCATTATTAAGAATTATTTATTTTCATTTCCTAATTTGTTTTTGAAAGGTTTCGTCCACCTAAACAACAGCCCACCAACAACAAAACGCAAAACCAAGTTGGTAAACTTTTCACCTAAAACCTTTCACCAATTATTTTATATTTTTAAACACTTTTTTCTTTTTTTTACACTATTGATTAAATGATGAAAAACATTTTGACAATAATTTTATCAATGACAAAAGTAATTTATAAGATTCTAGGATCACTCCCTTTAAATCTCTTCTGTTTTTCTAACATCTTTATCAATTCACGAGGCTCCTATATAATAATAATAACAATAACAACACTAATAAAAAAGGTTAGGTTTCTTTTAAACATAAAATTCAAATTTATATCTAATATATTATATAATTTCTAGAATATCTTTAACATTTGGAAAAATTATTAAACAAAAATTTAGAATTTTAGTTTTCCTTCTTGTAGTTTTAACTTTTATATAATTAAGTATACAAACACCATCACCACACTTAATATCCACTTTTTACTTATATTTTGAAACAAAGTGGTTTACTATTTTAAAAAACAGATCAAAATACGAAATTATTATCAAATGAAATAAATTTATTAAAATTTTTTTAAAAATCATAAGTGTAATAAACCGAAAAGTTTATAGTATCTAATAAGTATCCTAGAAGATGAAATTTAGTTGTAAATATTTGTCTATCATAACCGAAAACTTTACAATAACTTACGTTTTCCATAAAATTACAACATTAAACTTCCGAGTGTTAAACTTTATATTTAATATTAAGATAAATATATTTGCCCACGTGGCATAACAATAATAACCGTTGGATGATTGTAGGGATACGGAATGACGGAGGCGGGTCCAGTACTAACAATGGGATTAGCATTTGCAAAGGAACCGTTTCCGATGAAACCAGGCGCCTGCGGCACCGTCGTCAGAAACGCCGAGATGAAGATCGTCGACACCGAAACCGGAAGTTCCTTGCCTCGTAACACGCCGGGAGAGATATGCATCAGAGGCGATCAAATCATGAAAGGCTATCTCAACAATCCGGAGGCAACGGCGGCCACAATCGACAAGGATGGGTGGTTGCACACCGGGGATATCGGATTCATCGACGAAGATGACGAGCTGTTCATTGTGGATCGGTTGAAGGAACTGATTAAGTACAAGGGATTTCAAGTGGCTCCGGCGGAACTGGAAGCTCTGTTGTTGACTCATCCGGTCATCTCTGATGCCGCCGTTGTTCCGTAAGAATAATGAATTGAATTTGGATACCGGCTGGGTGATGATTGTTGGGTTAATTTTGTGTGGATTGATATTGCAGAATGAAAGATGAACAAGCTGGAGAAGTTCCGGTCGCGTTCGTTGTGAAATTGAAGAATTCTGAAGCCACTGAAGATGAAATTAAACAATTTATTTCAAAACAGGTATCTGTTTTTTTTTTTACTTAACAAATTTGAATTCTATTTTTTAAAATTATTATCTGCTTCTTATAATTTTTTCTGAAATTATATTGACATCACATTTTATTGATATTTCTAGTCATTCTTGCAAAATTAAGATATTACAAATTGATGTTAGTTGGAGAATGCAATATTTATGCTAAAAGATTAATTATATATATATATATATATATATATATTATTTATTTTTTTTTTTTGTTGTTGTGTGTATATANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTTGTGTGTGTGGAATATTAATTAGGAATTTAAAATTAAAAAATACGTGCAGGTGGTTTTCTACAAAAAGATAAACCGGGTGTTTTTCATAGACGCAATTCCCAAATCGCCCTCGGGAAAGATCTTGAGGAAGGAGTTGAGGGCAAAGTTGGCAGCTGCATTTCCAAATTCTAATTAACTCTCTCTCTCTCTTCTTAATTTCACAACACACACACACACACTCAATGCTGCTAATGATGATGTAAGTTCTTCTTCTTCTTCATTTTTTTTTTTTTTAAATTTTAATTTTTAATTTTCCCTCTTGTTGTTTACTTGTTCATTTGGAAAACAAGAAAGTTGTATTTTACATTGGAATTTGTTGTACTTGTATGCAACTATCTAAAGACCAAGCTCTATATATACATAATATATAAAGAAGGTGACTTATTTCGAGTTTCGACTCGTATATGTGTATATTAATTACCCACTAATACTATTTTTACATTTTTTTCTTCTTTTGTATT

mRNA sequence

TTCCATTCTTCGAACTCAGAAGAACAATAAACCTTCAAGAAAAAAGAAAGTTATCTGTTCTTCTTCTTCTTCTTCTTCTTCTTCAATTCGGCGTTGAAATGGGAATTGAAACAGTTGAGAATGACGTTATTTTCCGGTCAAAACTACCGGACATCTACATCCCAAATCATCTTCCTCTGCATTCCTACTGCCTACAAGAAAACGCCGCCAAAATAGGCCACCGAACTTGCTTAATCAACGGCGTTACCGGCGAATCCTTCACCTTCAACGACGTCGATCTCACTGCACGCAAGGTCGCCTCCGGATTAAACAAACTCGGCATTACAAAACGCGACGTAATCATGCTCCTCCTCCCAAATTCACCGGAGTTCGTCTTCGCTTTCCTCGGGGCCTCCCACCTCGGCGCAATCATGACGGCCGCAAACCCTTTCTTCACGGCAGCAGAAATCGCAAAGCAAGCAAAAGGATCAAAAGCAAAACTAATCATAACGCAATCGTCCTACTACGAAAAGTTGAAGGAAATAACAGAGGAATTGCCAGAGGTTAAAATCATGACCGTCGATTCTCCTCACGATGGTTGTTTATGGTTCGGTGATCTAATTAAAGCCGACGAAAGAGAGATTCCGAGGGTGGAAATCGACCCAGATGACGTGGTTGCGCTGCCGTATTCGTCGGGAACGACGGGATTGCCAAAGGGAGTGATGCTTACACATAAAAGTTTGGTTACGAGTGTGGCTCAGCAAGTGGATGGGGAAAATCCAAATCTGTATTATGGAAATGAGGATGTGATTCTGTGTGTTTTGCCGTTGTTTCATATTTATTCTTTGAATTCAGTTTTGCTGTGTGGATTAAGAGCTGGAAGTACCATTTTGATTATGCCCAAATTTGAGATCGGTTTGCTTTTGCAATTGGTGGAGAAATATGGAGTTACCGTGGCGCCCATCGTGCCGCCCATCGTTCTGGCTATTGCAAAGTCGCCGGAGCTAGAGAAGTATGACCTTTCGTCGATCAGAGTTATTAAATCCGGCGGTGCACCGCTTGGGAAGGAGCTTGAAGATACCGTAAGAGCCAAGTTTCCTAAGGCTGTGCTTGGACAGGGATACGGAATGACGGAGGCGGGTCCAGTACTAACAATGGGATTAGCATTTGCAAAGGAACCGTTTCCGATGAAACCAGGCGCCTGCGGCACCGTCGTCAGAAACGCCGAGATGAAGATCGTCGACACCGAAACCGGAAGTTCCTTGCCTCGTAACACGCCGGGAGAGATATGCATCAGAGGCGATCAAATCATGAAAGGCTATCTCAACAATCCGGAGGCAACGGCGGCCACAATCGACAAGGATGGGTGGTTGCACACCGGGGATATCGGATTCATCGACGAAGATGACGAGCTGTTCATTGTGGATCGGTTGAAGGAACTGATTAAGTACAAGGGATTTCAAGTGGCTCCGGCGGAACTGGAAGCTCTGTTGTTGACTCATCCGGTCATCTCTGATGCCGCCGTTGTTCCAATGAAAGATGAACAAGCTGGAGAAGTTCCGGTCGCGTTCGTTGTGAAATTGAAGAATTCTGAAGCCACTGAAGATGAAATTAAACAATTTATTTCAAAACAGGTGGTTTTCTACAAAAAGATAAACCGGGTGTTTTTCATAGACGCAATTCCCAAATCGCCCTCGGGAAAGATCTTGAGGAAGGAGTTGAGGGCAAAGTTGGCAGCTGCATTTCCAAATTCTAATTAACTCTCTCTCTCTCTTCTTAATTTCACAACACACACACACACACTCAATGCTGCTAATGATGATGTAAGTTCTTCTTCTTCTTCATTTTTTTTTTTTTTAaATTTTAATTTTTAATTTTCCCTCTTGTTGTTTACTTGTTCATTTGGAAAACAAGAAAGTTGTATTTTACATTGGAATTTGTTGTACTTGTATGCAACTATCTAAAGACCAAGCTCTATATATACATAATATATAAAGAAGGTGACTTATTTCGAGTTTCGACTCGTATATGTGTATATTAATTACCCACTAATACTATTTTTACATTTTTTTCTTCTTTTGTATT

Coding sequence (CDS)

ATGGGAATTGAAACAGTTGAGAATGACGTTATTTTCCGGTCAAAACTACCGGACATCTACATCCCAAATCATCTTCCTCTGCATTCCTACTGCCTACAAGAAAACGCCGCCAAAATAGGCCACCGAACTTGCTTAATCAACGGCGTTACCGGCGAATCCTTCACCTTCAACGACGTCGATCTCACTGCACGCAAGGTCGCCTCCGGATTAAACAAACTCGGCATTACAAAACGCGACGTAATCATGCTCCTCCTCCCAAATTCACCGGAGTTCGTCTTCGCTTTCCTCGGGGCCTCCCACCTCGGCGCAATCATGACGGCCGCAAACCCTTTCTTCACGGCAGCAGAAATCGCAAAGCAAGCAAAAGGATCAAAAGCAAAACTAATCATAACGCAATCGTCCTACTACGAAAAGTTGAAGGAAATAACAGAGGAATTGCCAGAGGTTAAAATCATGACCGTCGATTCTCCTCACGATGGTTGTTTATGGTTCGGTGATCTAATTAAAGCCGACGAAAGAGAGATTCCGAGGGTGGAAATCGACCCAGATGACGTGGTTGCGCTGCCGTATTCGTCGGGAACGACGGGATTGCCAAAGGGAGTGATGCTTACACATAAAAGTTTGGTTACGAGTGTGGCTCAGCAAGTGGATGGGGAAAATCCAAATCTGTATTATGGAAATGAGGATGTGATTCTGTGTGTTTTGCCGTTGTTTCATATTTATTCTTTGAATTCAGTTTTGCTGTGTGGATTAAGAGCTGGAAGTACCATTTTGATTATGCCCAAATTTGAGATCGGTTTGCTTTTGCAATTGGTGGAGAAATATGGAGTTACCGTGGCGCCCATCGTGCCGCCCATCGTTCTGGCTATTGCAAAGTCGCCGGAGCTAGAGAAGTATGACCTTTCGTCGATCAGAGTTATTAAATCCGGCGGTGCACCGCTTGGGAAGGAGCTTGAAGATACCGTAAGAGCCAAGTTTCCTAAGGCTGTGCTTGGACAGGGATACGGAATGACGGAGGCGGGTCCAGTACTAACAATGGGATTAGCATTTGCAAAGGAACCGTTTCCGATGAAACCAGGCGCCTGCGGCACCGTCGTCAGAAACGCCGAGATGAAGATCGTCGACACCGAAACCGGAAGTTCCTTGCCTCGTAACACGCCGGGAGAGATATGCATCAGAGGCGATCAAATCATGAAAGGCTATCTCAACAATCCGGAGGCAACGGCGGCCACAATCGACAAGGATGGGTGGTTGCACACCGGGGATATCGGATTCATCGACGAAGATGACGAGCTGTTCATTGTGGATCGGTTGAAGGAACTGATTAAGTACAAGGGATTTCAAGTGGCTCCGGCGGAACTGGAAGCTCTGTTGTTGACTCATCCGGTCATCTCTGATGCCGCCGTTGTTCCAATGAAAGATGAACAAGCTGGAGAAGTTCCGGTCGCGTTCGTTGTGAAATTGAAGAATTCTGAAGCCACTGAAGATGAAATTAAACAATTTATTTCAAAACAGGTGGTTTTCTACAAAAAGATAAACCGGGTGTTTTTCATAGACGCAATTCCCAAATCGCCCTCGGGAAAGATCTTGAGGAAGGAGTTGAGGGCAAAGTTGGCAGCTGCATTTCCAAATTCTAATTAA

Protein sequence

MGIETVENDVIFRSKLPDIYIPNHLPLHSYCLQENAAKIGHRTCLINGVTGESFTFNDVDLTARKVASGLNKLGITKRDVIMLLLPNSPEFVFAFLGASHLGAIMTAANPFFTAAEIAKQAKGSKAKLIITQSSYYEKLKEITEELPEVKIMTVDSPHDGCLWFGDLIKADEREIPRVEIDPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGNEDVILCVLPLFHIYSLNSVLLCGLRAGSTILIMPKFEIGLLLQLVEKYGVTVAPIVPPIVLAIAKSPELEKYDLSSIRVIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPGACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHTGDIGFIDEDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVPVAFVVKLKNSEATEDEIKQFISKQVVFYKKINRVFFIDAIPKSPSGKILRKELRAKLAAAFPNSN
Homology
[Close]
BLAST of MELO3C024886.2 vs. NCBI nr
Match: XP_004137646.2 (PREDICTED: 4-coumarate--CoA ligase 1 [Cucumis sativus])

HSP 1 Score: 1050.8 bits (2716), Expect = 1.6e-303
Identity = 526/546 (96.34%), Postives = 540/546 (98.90%), Query Frame = 0 

Query: 1   MGIETVENDVIFRSKLPDIYIPNHLPLHSYCLQENAAKIGHRTCLINGVTGESFTFNDVD 60
           MGIETVENDVIFRSKLPDIYIP HLPLHSYCLQENAAKIGHRTCLINGVTGESFT+NDVD
Sbjct: 1   MGIETVENDVIFRSKLPDIYIPKHLPLHSYCLQENAAKIGHRTCLINGVTGESFTYNDVD 60

Query: 61  LTARKVASGLNKLGITKRDVIMLLLPNSPEFVFAFLGASHLGAIMTAANPFFTAAEIAKQ 120
           LT RKVASGLNKLGITKRDVIMLLLPNSPEFVFAFLGAS+LGAIMTAANPFFTAAEI KQ
Sbjct: 61  LTTRKVASGLNKLGITKRDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIGKQ 120

Query: 121 AKGSKAKLIITQSSYYEKLKEITEELPEVKIMTVDSPHDGCLWFGDLIKADEREIPRVEI 180
           AKGSK+KLIITQSSYYEK+KEITEELPEVKIMTVDSP DGCLWFGDLIKADERE+PRV+I
Sbjct: 121 AKGSKSKLIITQSSYYEKVKEITEELPEVKIMTVDSPPDGCLWFGDLIKADEREVPRVDI 180

Query: 181 DPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGNEDVILCVLPLFHI 240
           DP+DVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYG+EDVILCVLPLFHI
Sbjct: 181 DPEDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGHEDVILCVLPLFHI 240

Query: 241 YSLNSVLLCGLRAGSTILIMPKFEIGLLLQLVEKYGVTVAPIVPPIVLAIAKSPELEKYD 300
           YSLNSVLLCGLRAGSTILIMPKFEIGLLLQL EKYGVTVAPIVPPIVLAIAKSPELEKYD
Sbjct: 241 YSLNSVLLCGLRAGSTILIMPKFEIGLLLQLAEKYGVTVAPIVPPIVLAIAKSPELEKYD 300

Query: 301 LSSIRVIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360
           LSSIR+IKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAK+PFPMKPG
Sbjct: 301 LSSIRIIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKDPFPMKPG 360

Query: 361 ACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420
           ACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT
Sbjct: 361 ACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420

Query: 421 GDIGFIDEDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480
           GDIGFID+DDE+FIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV
Sbjct: 421 GDIGFIDDDDEMFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480

Query: 481 PVAFVVKLKNSEATEDEIKQFISKQVVFYKKINRVFFIDAIPKSPSGKILRKELRAKLAA 540
           PVAFVVKLKNSEA+EDEIKQFISKQVVFYK+INRVFFIDAIPKSPSGKILRKELRAKLAA
Sbjct: 481 PVAFVVKLKNSEASEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAA 540

Query: 541 AFPNSN 547
           AFP SN
Sbjct: 541 AFPTSN 546

[Close]
BLAST of MELO3C024886.2 vs. NCBI nr
Match: XP_008462955.1 (PREDICTED: 4-coumarate--CoA ligase 1-like [Cucumis melo])

HSP 1 Score: 1004.6 bits (2596), Expect = 1.3e-289
Identity = 510/519 (98.27%), Postives = 513/519 (98.84%), Query Frame = 0 

Query: 1   MGIETVENDVIFRSKLPDIYIPNHLPLHSYCLQENAAKIGHRTCLINGVTGESFTFNDVD 60
           MGIETVENDVIFRSKLPDIYIPNHLPLHSYCLQENAAKIGHRTCLINGVTGESFTFNDVD
Sbjct: 1   MGIETVENDVIFRSKLPDIYIPNHLPLHSYCLQENAAKIGHRTCLINGVTGESFTFNDVD 60

Query: 61  LTARKVASGLNKLGITKRDVIMLLLPNSPEFVFAFLGASHLGAIMTAANPFFTAAEIAKQ 120
           LTARKVASGLNKLGITKRDVIMLLLPNSPEFVFAFLGASHLGAIMTAANPFFTAAEIAKQ
Sbjct: 61  LTARKVASGLNKLGITKRDVIMLLLPNSPEFVFAFLGASHLGAIMTAANPFFTAAEIAKQ 120

Query: 121 AKGSKAKLIITQSSYYEKLKEITEELPEVKIMTVDSPHDGCLWFGDLIKADEREIPRVEI 180
           AKGSKAKLIITQSSYYEKLKEITEELPEVKIMTVDSPHDGCLWFGDLIKADEREIPRVEI
Sbjct: 121 AKGSKAKLIITQSSYYEKLKEITEELPEVKIMTVDSPHDGCLWFGDLIKADEREIPRVEI 180

Query: 181 DPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGNEDVILCVLPLFHI 240
           DPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGNEDVILCVLPLFHI
Sbjct: 181 DPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGNEDVILCVLPLFHI 240

Query: 241 YSLNSVLLCGLRAGSTILIMPKFEIGLLLQLVEKYGVTVAPIVPPIVLAIAKSPELEKYD 300
           YSLNSVLLCGLRAGSTILIMPKFEIGLLLQLVEKYGVTVAPIVPPIVLAIAKSPELEKYD
Sbjct: 241 YSLNSVLLCGLRAGSTILIMPKFEIGLLLQLVEKYGVTVAPIVPPIVLAIAKSPELEKYD 300

Query: 301 LSSIRVIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360
           LSSIRVIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG
Sbjct: 301 LSSIRVIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360

Query: 361 ACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420
           ACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT
Sbjct: 361 ACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420

Query: 421 GDIGFIDEDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480
           GDIGFIDEDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV
Sbjct: 421 GDIGFIDEDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480

Query: 481 PVAFVVKLKNSEATEDEIKQFISKQV-VFY--KKINRVF 517
           PVAFVVKLKNSEATEDEIKQFISKQV VF+    +N +F
Sbjct: 481 PVAFVVKLKNSEATEDEIKQFISKQVSVFFYLTNLNSIF 519

[Close]
BLAST of MELO3C024886.2 vs. NCBI nr
Match: XP_022935389.1 (4-coumarate--CoA ligase 1-like [Cucurbita moschata])

HSP 1 Score: 981.9 bits (2537), Expect = 9.0e-283
Identity = 490/546 (89.74%), Postives = 517/546 (94.69%), Query Frame = 0 

Query: 1   MGIETVENDVIFRSKLPDIYIPNHLPLHSYCLQENAAKIGHRTCLINGVTGESFTFNDVD 60
           M +E ++ND IFRSKLPDIYIPNHLPLHSYCL EN AKIGHRTCLIN VTGESFT++DVD
Sbjct: 1   MAVEALQNDFIFRSKLPDIYIPNHLPLHSYCLHENLAKIGHRTCLINSVTGESFTYHDVD 60

Query: 61  LTARKVASGLNKLGITKRDVIMLLLPNSPEFVFAFLGASHLGAIMTAANPFFTAAEIAKQ 120
           L ARKVASGLNKLGI + DVIML+L NSPEFVFAFLGAS+ GAIMTAANPFFTAAEIAKQ
Sbjct: 61  LAARKVASGLNKLGIAQGDVIMLILQNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120

Query: 121 AKGSKAKLIITQSSYYEKLKEITEELPEVKIMTVDSPHDGCLWFGDLIKADEREIPRVEI 180
           AKGS AKLI+TQSSYYEK+KEITEELP+VKIMTVDSP DGCL F DLI+ADERE+P VEI
Sbjct: 121 AKGSNAKLIVTQSSYYEKVKEITEELPDVKIMTVDSPPDGCLSFADLIQADEREMPTVEI 180

Query: 181 DPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGNEDVILCVLPLFHI 240
           +PDDVVALPYSSGTTGLPKGVMLTH+ LVTSVAQQVDGENPNLYY NEDVILCVLPLFHI
Sbjct: 181 NPDDVVALPYSSGTTGLPKGVMLTHRGLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHI 240

Query: 241 YSLNSVLLCGLRAGSTILIMPKFEIGLLLQLVEKYGVTVAPIVPPIVLAIAKSPELEKYD 300
           YSLNSVLLCGLRAG+TILIMPKFEIG LLQLV+K+ V++APIVPPIVLAIAKSP+L+KYD
Sbjct: 241 YSLNSVLLCGLRAGTTILIMPKFEIGSLLQLVQKFKVSIAPIVPPIVLAIAKSPDLDKYD 300

Query: 301 LSSIRVIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360
           LSSIRVIK GGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG
Sbjct: 301 LSSIRVIKCGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360

Query: 361 ACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420
           ACGTVVRNAEMKIVDTE G SLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT
Sbjct: 361 ACGTVVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420

Query: 421 GDIGFIDEDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480
           GDIGFID+DDELFIVDRLKELIKYKGFQVAPAE+EALLLTHP ISDAAVVPMKDE+AGEV
Sbjct: 421 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAEVEALLLTHPAISDAAVVPMKDEEAGEV 480

Query: 481 PVAFVVKLKNSEATEDEIKQFISKQVVFYKKINRVFFIDAIPKSPSGKILRKELRAKLAA 540
           PVAFVV+LKNSE TEDEIKQFISKQVVFYK+I R F IDAIPKSPSGKILRKELRAKLAA
Sbjct: 481 PVAFVVRLKNSEVTEDEIKQFISKQVVFYKRIKRAFMIDAIPKSPSGKILRKELRAKLAA 540

Query: 541 AFPNSN 547
            FPNSN
Sbjct: 541 GFPNSN 546

[Close]
BLAST of MELO3C024886.2 vs. NCBI nr
Match: XP_023526871.1 (4-coumarate--CoA ligase 1-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 975.7 bits (2521), Expect = 6.5e-281
Identity = 487/546 (89.19%), Postives = 514/546 (94.14%), Query Frame = 0 

Query: 1   MGIETVENDVIFRSKLPDIYIPNHLPLHSYCLQENAAKIGHRTCLINGVTGESFTFNDVD 60
           M +E ++ND IFRSKLP IYIPNHLPLHSYCL EN AKIGHRTCLIN VTGESFT++DVD
Sbjct: 1   MAVEALQNDFIFRSKLPHIYIPNHLPLHSYCLHENLAKIGHRTCLINSVTGESFTYHDVD 60

Query: 61  LTARKVASGLNKLGITKRDVIMLLLPNSPEFVFAFLGASHLGAIMTAANPFFTAAEIAKQ 120
           L ARKVASGLNKLGI + DVIMLLL NSPEFVFAFLGAS+ GAIMTAANPFFTAAEIAKQ
Sbjct: 61  LAARKVASGLNKLGIAQGDVIMLLLQNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120

Query: 121 AKGSKAKLIITQSSYYEKLKEITEELPEVKIMTVDSPHDGCLWFGDLIKADEREIPRVEI 180
           AKGS AKLI+TQSSYYEK+KEITEE+P+VKIMTVDSP DGCL F DLI+ADERE+P VEI
Sbjct: 121 AKGSNAKLIVTQSSYYEKVKEITEEVPDVKIMTVDSPPDGCLSFADLIQADEREMPTVEI 180

Query: 181 DPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGNEDVILCVLPLFHI 240
           +PDDVVALPYSSGTTGLPKGVMLTH+ LVTSVAQQVDGENPNLYY NEDVILCVLPLFHI
Sbjct: 181 NPDDVVALPYSSGTTGLPKGVMLTHRGLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHI 240

Query: 241 YSLNSVLLCGLRAGSTILIMPKFEIGLLLQLVEKYGVTVAPIVPPIVLAIAKSPELEKYD 300
           YSLNSVLLCGLRAG+TILIMPKFEIG LLQLV+K+ V++APIVPPIVLAIAKSP+L+KYD
Sbjct: 241 YSLNSVLLCGLRAGTTILIMPKFEIGSLLQLVQKFKVSIAPIVPPIVLAIAKSPDLDKYD 300

Query: 301 LSSIRVIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360
           LSSIRVIK GGAPLGKELEDTVR KFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG
Sbjct: 301 LSSIRVIKCGGAPLGKELEDTVRTKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360

Query: 361 ACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420
           ACGTVVRNAEMKIVDTE G SLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT
Sbjct: 361 ACGTVVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420

Query: 421 GDIGFIDEDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480
           GDIGFID+DDELFIVDRLKELIKYKGFQVAPAE+EALLLTHP ISDAAVVPMKDE+AGEV
Sbjct: 421 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAEVEALLLTHPAISDAAVVPMKDEEAGEV 480

Query: 481 PVAFVVKLKNSEATEDEIKQFISKQVVFYKKINRVFFIDAIPKSPSGKILRKELRAKLAA 540
           PVAFVV+LKNSE TEDEIKQFISKQVVFYK+I R F IDAIPKSPSGKILRKELRAKLA 
Sbjct: 481 PVAFVVRLKNSEVTEDEIKQFISKQVVFYKRIKRAFMIDAIPKSPSGKILRKELRAKLAT 540

Query: 541 AFPNSN 547
            FPNSN
Sbjct: 541 GFPNSN 546

[Close]
BLAST of MELO3C024886.2 vs. NCBI nr
Match: XP_022983944.1 (4-coumarate--CoA ligase 1-like [Cucurbita maxima])

HSP 1 Score: 973.4 bits (2515), Expect = 3.2e-280
Identity = 487/546 (89.19%), Postives = 514/546 (94.14%), Query Frame = 0 

Query: 1   MGIETVENDVIFRSKLPDIYIPNHLPLHSYCLQENAAKIGHRTCLINGVTGESFTFNDVD 60
           M +E ++ND IFRSKLPDIYIPNHLPLHSYCL EN AKIGHRTCLIN VT ESFT++DVD
Sbjct: 1   MAVEALQNDFIFRSKLPDIYIPNHLPLHSYCLHENLAKIGHRTCLINAVTAESFTYHDVD 60

Query: 61  LTARKVASGLNKLGITKRDVIMLLLPNSPEFVFAFLGASHLGAIMTAANPFFTAAEIAKQ 120
           L ARKVASGLNKLGI + DVI+LLL NSPEFVFAFLGAS+ GAIMTAANPFFTAAEIAKQ
Sbjct: 61  LAARKVASGLNKLGIAQGDVILLLLQNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120

Query: 121 AKGSKAKLIITQSSYYEKLKEITEELPEVKIMTVDSPHDGCLWFGDLIKADEREIPRVEI 180
           AKGS AKLI+TQSSYYEK+KEITEELP+VKIMTVDSP DGCL F DLI+ADERE+P VEI
Sbjct: 121 AKGSNAKLIVTQSSYYEKVKEITEELPDVKIMTVDSPPDGCLSFADLIQADEREMPTVEI 180

Query: 181 DPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGNEDVILCVLPLFHI 240
           +PDDVVALPYSSGTTGLPKGVMLTH+ LVTSVAQQVDGENPNLYY NEDVILCVLPLFHI
Sbjct: 181 NPDDVVALPYSSGTTGLPKGVMLTHRGLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHI 240

Query: 241 YSLNSVLLCGLRAGSTILIMPKFEIGLLLQLVEKYGVTVAPIVPPIVLAIAKSPELEKYD 300
           YSLNSVLLCGLRAG+TILIMPKFEIG LLQLV+K+ V++APIVPPIVLAIAKSP+L+KYD
Sbjct: 241 YSLNSVLLCGLRAGTTILIMPKFEIGSLLQLVQKFKVSIAPIVPPIVLAIAKSPDLDKYD 300

Query: 301 LSSIRVIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360
           LSSIRVIK GGAPLGKELED+VRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG
Sbjct: 301 LSSIRVIKCGGAPLGKELEDSVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360

Query: 361 ACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420
           ACGTVVRNAEMKIVDTE G SLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT
Sbjct: 361 ACGTVVRNAEMKIVDTENGCSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420

Query: 421 GDIGFIDEDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480
           GDIGFID+DDELFIVDRLKELIKYKGFQVAPAELEALLLTHP ISDAAVVPMKDE+AGEV
Sbjct: 421 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPAISDAAVVPMKDEEAGEV 480

Query: 481 PVAFVVKLKNSEATEDEIKQFISKQVVFYKKINRVFFIDAIPKSPSGKILRKELRAKLAA 540
           PVAFVV+LKNSE TEDEIKQFISKQVVFYK+I R F IDAIPKSPSGKILRKELRAKLA 
Sbjct: 481 PVAFVVRLKNSEVTEDEIKQFISKQVVFYKRIKRAFMIDAIPKSPSGKILRKELRAKLAT 540

Query: 541 AFPNSN 547
            FP SN
Sbjct: 541 GFPISN 546

[Close]
BLAST of MELO3C024886.2 vs. TAIR10
Match: AT3G21240.1 (4-coumarate:CoA ligase 2)

HSP 1 Score: 766.9 bits (1979), Expect = 8.3e-222
Identity = 382/541 (70.61%), Postives = 455/541 (84.10%), Query Frame = 0 

Query: 8   NDVIFRSKLPDIYIPNHLPLHSYCLQENAAKIGHRTCLINGVTGESFTFNDVDLTARKVA 67
           NDVIFRS+LPDIYIPNHLPLH Y + EN ++   + CLING TGE +T+ DV +T+RK+A
Sbjct: 19  NDVIFRSRLPDIYIPNHLPLHDY-IFENISEFAAKPCLINGPTGEVYTYADVHVTSRKLA 78

Query: 68  SGLNKLGITKRDVIMLLLPNSPEFVFAFLGASHLGAIMTAANPFFTAAEIAKQAKGSKAK 127
           +GL+ LG+ + DV+M+LLPNSPE V  FL AS +GAI T+ANPFFT AEI+KQAK S AK
Sbjct: 79  AGLHNLGVKQHDVVMILLPNSPEVVLTFLAASFIGAITTSANPFFTPAEISKQAKASAAK 138

Query: 128 LIITQSSYYEKLKEITEELPEVKIMTVDSP--HDGCLWFGDLIKADEREIPRV--EIDPD 187
           LI+TQS Y +K+K +  +   V I+T DS    + CL F +L +++E  +  +  +I P+
Sbjct: 139 LIVTQSRYVDKIKNLQND--GVLIVTTDSDAIPENCLRFSELTQSEEPRVDSIPEKISPE 198

Query: 188 DVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGNEDVILCVLPLFHIYSL 247
           DVVALP+SSGTTGLPKGVMLTHK LVTSVAQQVDGENPNLY+  +DVILCVLP+FHIY+L
Sbjct: 199 DVVALPFSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFNRDDVILCVLPMFHIYAL 258

Query: 248 NSVLLCGLRAGSTILIMPKFEIGLLLQLVEKYGVTVAPIVPPIVLAIAKSPELEKYDLSS 307
           NS++LC LR G+TILIMPKFEI LLL+ +++  VTVA +VPPIVLAIAKSPE EKYDLSS
Sbjct: 259 NSIMLCSLRVGATILIMPKFEITLLLEQIQRCKVTVAMVVPPIVLAIAKSPETEKYDLSS 318

Query: 308 IRVIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPGACG 367
           +R++KSG APLGKELED + AKFP A LGQGYGMTEAGPVL M L FAKEPFP+K GACG
Sbjct: 319 VRMVKSGAAPLGKELEDAISAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKSGACG 378

Query: 368 TVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHTGDI 427
           TVVRNAEMKI+D +TG SLPRN PGEICIRG+QIMKGYLN+P ATA+TIDKDGWLHTGD+
Sbjct: 379 TVVRNAEMKILDPDTGDSLPRNKPGEICIRGNQIMKGYLNDPLATASTIDKDGWLHTGDV 438

Query: 428 GFIDEDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVPVA 487
           GFID+DDELFIVDRLKELIKYKGFQVAPAELE+LL+ HP I+D AVV MK+E AGEVPVA
Sbjct: 439 GFIDDDDELFIVDRLKELIKYKGFQVAPAELESLLIGHPEINDVAVVAMKEEDAGEVPVA 498

Query: 488 FVVKLKNSEATEDEIKQFISKQVVFYKKINRVFFIDAIPKSPSGKILRKELRAKLAAAFP 545
           FVV+ K+S  +EDEIKQF+SKQVVFYK+IN+VFF D+IPK+PSGKILRK+LRA+LA    
Sbjct: 499 FVVRSKDSNISEDEIKQFVSKQVVFYKRINKVFFTDSIPKAPSGKILRKDLRARLANGLM 556

[Close]
BLAST of MELO3C024886.2 vs. TAIR10
Match: AT1G51680.1 (4-coumarate:CoA ligase 1)

HSP 1 Score: 757.7 bits (1955), Expect = 5.0e-219
Identity = 385/540 (71.30%), Postives = 445/540 (82.41%), Query Frame = 0 

Query: 8   NDVIFRSKLPDIYIPNHLPLHSYCLQENAAKIGHRTCLINGVTGESFTFNDVDLTARKVA 67
           +DVIFRSKLPDIYIPNHL LH Y  Q N ++   + CLING TG  +T++DV + +R++A
Sbjct: 21  SDVIFRSKLPDIYIPNHLSLHDYIFQ-NISEFATKPCLINGPTGHVYTYSDVHVISRQIA 80

Query: 68  SGLNKLGITKRDVIMLLLPNSPEFVFAFLGASHLGAIMTAANPFFTAAEIAKQAKGSKAK 127
           +  +KLG+ + DV+MLLLPN PEFV +FL AS  GA  TAANPFFT AEIAKQAK S  K
Sbjct: 81  ANFHKLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAKASNTK 140

Query: 128 LIITQSSYYEKLKEITEELPEVKIMTVDSPH-----DGCLWFGDLIKAD---EREIPRVE 187
           LIIT++ Y +K+K +  +   V I+ +D        +GCL F +L ++       I  VE
Sbjct: 141 LIITEARYVDKIKPLQND-DGVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEVIDSVE 200

Query: 188 IDPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGNEDVILCVLPLFH 247
           I PDDVVALPYSSGTTGLPKGVMLTHK LVTSVAQQVDGENPNLY+ ++DVILCVLP+FH
Sbjct: 201 ISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLPMFH 260

Query: 248 IYSLNSVLLCGLRAGSTILIMPKFEIGLLLQLVEKYGVTVAPIVPPIVLAIAKSPELEKY 307
           IY+LNS++LCGLR G+ ILIMPKFEI LLL+L+++  VTVAP+VPPIVLAIAKS E EKY
Sbjct: 261 IYALNSIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAKSSETEKY 320

Query: 308 DLSSIRVIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKP 367
           DLSSIRV+KSG APLGKELED V AKFP A LGQGYGMTEAGPVL M L FAKEPFP+K 
Sbjct: 321 DLSSIRVVKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKS 380

Query: 368 GACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLH 427
           GACGTVVRNAEMKIVD +TG SL RN PGEICIRG QIMKGYLNNP ATA TIDKDGWLH
Sbjct: 381 GACGTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKGYLNNPAATAETIDKDGWLH 440

Query: 428 TGDIGFIDEDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGE 487
           TGDIG ID+DDELFIVDRLKELIKYKGFQVAPAELEALL+ HP I+D AVV MK+E AGE
Sbjct: 441 TGDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVVAMKEEAAGE 500

Query: 488 VPVAFVVKLKNSEATEDEIKQFISKQVVFYKKINRVFFIDAIPKSPSGKILRKELRAKLA 540
           VPVAFVVK K+SE +ED++KQF+SKQVVFYK+IN+VFF ++IPK+PSGKILRK+LRAKLA
Sbjct: 501 VPVAFVVKSKDSELSEDDVKQFVSKQVVFYKRINKVFFTESIPKAPSGKILRKDLRAKLA 558

[Close]
BLAST of MELO3C024886.2 vs. TAIR10
Match: AT1G65060.1 (4-coumarate:CoA ligase 3)

HSP 1 Score: 686.8 bits (1771), Expect = 1.1e-197
Identity = 342/531 (64.41%), Postives = 427/531 (80.41%), Query Frame = 0 

Query: 11  IFRSKLPDIYIPNHLPLHSYCLQENAAKIGHRTCLINGVTGESFTFNDVDLTARKVASGL 70
           IFRSKLPDI IPNHLPLH+YC  E  + +  + CLI G TG+S+T+ +  L  R+VASGL
Sbjct: 34  IFRSKLPDIDIPNHLPLHTYCF-EKLSSVSDKPCLIVGSTGKSYTYGETHLICRRVASGL 93

Query: 71  NKLGITKRDVIMLLLPNSPEFVFAFLGASHLGAIMTAANPFFTAAEIAKQAKGSKAKLII 130
            KLGI K DVIM+LL NS EFVF+F+GAS +GA+ T ANPF+T+ E+ KQ K S AKLII
Sbjct: 94  YKLGIRKGDVIMILLQNSAEFVFSFMGASMIGAVSTTANPFYTSQELYKQLKSSGAKLII 153

Query: 131 TQSSYYEKLKEITEELPEVKIMTVDSP-HDGCLWFGDLIKADERE--IPRVEIDPDDVVA 190
           T S Y +KLK + E L    ++T D P  + CL F  LI  DE       V+I  DD  A
Sbjct: 154 THSQYVDKLKNLGENL---TLITTDEPTPENCLPFSTLITDDETNPFQETVDIGGDDAAA 213

Query: 191 LPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGNEDVILCVLPLFHIYSLNSVL 250
           LP+SSGTTGLPKGV+LTHKSL+TSVAQQVDG+NPNLY  + DVILCVLPLFHIYSLNSVL
Sbjct: 214 LPFSSGTTGLPKGVVLTHKSLITSVAQQVDGDNPNLYLKSNDVILCVLPLFHIYSLNSVL 273

Query: 251 LCGLRAGSTILIMPKFEIGLLLQLVEKYGVTVAPIVPPIVLAIAKSPELEKYDLSSIRVI 310
           L  LR+G+T+L+M KFEIG LL L++++ VT+A +VPP+V+A+AK+P +  YDLSS+R +
Sbjct: 274 LNSLRSGATVLLMHKFEIGALLDLIQRHRVTIAALVPPLVIALAKNPTVNSYDLSSVRFV 333

Query: 311 KSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPGACGTVVR 370
            SG APLGKEL+D++R + P+A+LGQGYGMTEAGPVL+M L FAKEP P K G+CGTVVR
Sbjct: 334 LSGAAPLGKELQDSLRRRLPQAILGQGYGMTEAGPVLSMSLGFAKEPIPTKSGSCGTVVR 393

Query: 371 NAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHTGDIGFID 430
           NAE+K+V  ET  SL  N PGEICIRG QIMK YLN+PEAT+ATID++GWLHTGDIG++D
Sbjct: 394 NAELKVVHLETRLSLGYNQPGEICIRGQQIMKEYLNDPEATSATIDEEGWLHTGDIGYVD 453

Query: 431 EDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVPVAFVVK 490
           EDDE+FIVDRLKE+IK+KGFQV PAELE+LL+ H  I+DAAVVP  DE AGEVPVAFVV+
Sbjct: 454 EDDEIFIVDRLKEVIKFKGFQVPPAELESLLINHHSIADAAVVPQNDEVAGEVPVAFVVR 513

Query: 491 LKNSEATEDEIKQFISKQVVFYKKINRVFFIDAIPKSPSGKILRKELRAKL 539
              ++ TE+++K++++KQVVFYK++++VFF+ +IPKSPSGKILRK+L+AKL
Sbjct: 514 SNGNDITEEDVKEYVAKQVVFYKRLHKVFFVASIPKSPSGKILRKDLKAKL 560

[Close]
BLAST of MELO3C024886.2 vs. TAIR10
Match: AT3G21230.1 (4-coumarate:CoA ligase 5)

HSP 1 Score: 673.3 bits (1736), Expect = 1.2e-193
Identity = 331/545 (60.73%), Postives = 418/545 (76.70%), Query Frame = 0 

Query: 4   ETVENDVIFRSKLPDIYIPNHLPLHSYCLQE---NAAKIGHRTCLINGVTGESFTFNDVD 63
           E   +D IFRSKLPDI+IPNHLPL  Y  Q    +       TC+I+G TG   T+ DV 
Sbjct: 21  EEPSHDFIFRSKLPDIFIPNHLPLTDYVFQRFSGDGDGDSSTTCIIDGATGRILTYADVQ 80

Query: 64  LTARKVASGLNKLGITKRDVIMLLLPNSPEFVFAFLGASHLGAIMTAANPFFTAAEIAKQ 123
              R++A+G+++LGI   DV+MLLLPNSPEF  +FL  ++LGA+ T ANPF+T  EIAKQ
Sbjct: 81  TNMRRIAAGIHRLGIRHGDVVMLLLPNSPEFALSFLAVAYLGAVSTTANPFYTQPEIAKQ 140

Query: 124 AKGSKAKLIITQSSYYEKLKEITEELPEVKIM-------TVDSPHDGCLWFGDLIKADER 183
           AK S AK+IIT+    +KL  +  +   +  +        V S  DGC+ F +L +ADE 
Sbjct: 141 AKASAAKMIITKKCLVDKLTNLKNDGVLIVCLDDDGDNGVVSSSDDGCVSFTELTQADET 200

Query: 184 EIPRVEIDPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGNEDVILC 243
           E+ + +I P+D VA+PYSSGTTGLPKGVM+THK LVTS+AQ+VDGENPNL +   DVILC
Sbjct: 201 ELLKPKISPEDTVAMPYSSGTTGLPKGVMITHKGLVTSIAQKVDGENPNLNFTANDVILC 260

Query: 244 VLPLFHIYSLNSVLLCGLRAGSTILIMPKFEIGLLLQLVEKYGVTVAPIVPPIVLAIAKS 303
            LP+FHIY+L++++L  +R G+ +LI+P+FE+ L+++L+++Y VTV P+ PP+VLA  KS
Sbjct: 261 FLPMFHIYALDALMLSAMRTGAALLIVPRFELNLVMELIQRYKVTVVPVAPPVVLAFIKS 320

Query: 304 PELEKYDLSSIRVIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKE 363
           PE E+YDLSS+R++ SG A L KELED VR KFP A+ GQGYGMTE+G V    LAFAK 
Sbjct: 321 PETERYDLSSVRIMLSGAATLKKELEDAVRLKFPNAIFGQGYGMTESGTV-AKSLAFAKN 380

Query: 364 PFPMKPGACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATID 423
           PF  K GACGTV+RNAEMK+VDTETG SLPRN  GEIC+RG Q+MKGYLN+PEATA TID
Sbjct: 381 PFKTKSGACGTVIRNAEMKVVDTETGISLPRNKSGEICVRGHQLMKGYLNDPEATARTID 440

Query: 424 KDGWLHTGDIGFIDEDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMK 483
           KDGWLHTGDIGF+D+DDE+FIVDRLKELIK+KG+QVAPAELEALL++HP I DAAVV MK
Sbjct: 441 KDGWLHTGDIGFVDDDDEIFIVDRLKELIKFKGYQVAPAELEALLISHPSIDDAAVVAMK 500

Query: 484 DEQAGEVPVAFVVKLKNSEATEDEIKQFISKQVVFYKKINRVFFIDAIPKSPSGKILRKE 539
           DE A EVPVAFV + + S+ TED++K +++KQVV YK+I  VFFI+ IPK+ SGKILRK+
Sbjct: 501 DEVADEVPVAFVARSQGSQLTEDDVKSYVNKQVVHYKRIKMVFFIEVIPKAVSGKILRKD 560

[Close]
BLAST of MELO3C024886.2 vs. TAIR10
Match: AT4G05160.1 (AMP-dependent synthetase and ligase family protein)

HSP 1 Score: 423.7 bits (1088), Expect = 1.7e-118
Identity = 237/543 (43.65%), Postives = 341/543 (62.80%), Query Frame = 0 

Query: 9   DVIFRSKLPDIYIPN--HLPLHSYCLQENAAKIGHRTCLINGVTGESFTFNDVDLTARKV 68
           D I+RS  P + +P   +  L S+ L  N++    +  + +  TG+S TF+ +     ++
Sbjct: 9   DGIYRSLRPTLVLPKDPNTSLVSF-LFRNSSSYPSKLAIADSDTGDSLTFSQLKSAVARL 68

Query: 69  ASGLNKLGITKRDVIMLLLPNSPEFVFAFLGASHLGAIMTAANPFFTAAEIAKQAKGSKA 128
           A G ++LGI K DV+++  PNS +F   FL  + +G + T ANP +T  E++KQ K S  
Sbjct: 69  AHGFHRLGIRKNDVVLIFAPNSYQFPLCFLAVTAIGGVFTTANPLYTVNEVSKQIKDSNP 128

Query: 129 KLIITQSSYYEKLKEITEELPEVKIMTVDS------PHDGCLWFGDLIKADE--REIPRV 188
           K+II+ +  ++K+K    +LP V + + D+       +   L F ++++  E   E P V
Sbjct: 129 KIIISVNQLFDKIKGF--DLPVVLLGSKDTVEIPPGSNSKILSFDNVMELSEPVSEYPFV 188

Query: 189 EIDPDDVVALPYSSGTTGLPKGVMLTH-----KSLVTSVAQQVDGENPNLYYGNEDVILC 248
           EI   D  AL YSSGTTG  KGV LTH      SL+ ++ Q + GE    Y+G   V LC
Sbjct: 189 EIKQSDTAALLYSSGTTGTSKGVELTHGNFIAASLMVTMDQDLMGE----YHG---VFLC 248

Query: 249 VLPLFHIYSLNSVLLCGLRAGSTILIMPKFEIGLLLQLVEKYGVTVAPIVPPIVLAIAKS 308
            LP+FH++ L  +    L+ G+ ++ M +FE+ L+L+ +EK+ VT   +VPP+ LA++K 
Sbjct: 249 FLPMFHVFGLAVITYSQLQRGNALVSMARFELELVLKNIEKFRVTHLWVVPPVFLALSKQ 308

Query: 309 PELEKYDLSSIRVIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKE 368
             ++K+DLSS++ I SG APLGK+L +      P  +L QGYGMTE     T G+   ++
Sbjct: 309 SIVKKFDLSSLKYIGSGAAPLGKDLMEECGRNIPNVLLMQGYGMTE-----TCGIVSVED 368

Query: 369 PFPMK--PGACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAAT 428
           P   K   G+ G +    E +IV  ETG S P N  GEI +RG  +MKGYLNNP+AT  T
Sbjct: 369 PRLGKRNSGSAGMLAPGVEAQIVSVETGKSQPPNQQGEIWVRGPNMMKGYLNNPQATKET 428

Query: 429 IDKDGWLHTGDIGFIDEDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVP 488
           IDK  W+HTGD+G+ +ED  L++VDR+KELIKYKGFQVAPAELE LL++HP I DA V+P
Sbjct: 429 IDKKSWVHTGDLGYFNEDGNLYVVDRIKELIKYKGFQVAPAELEGLLVSHPDILDAVVIP 488

Query: 489 MKDEQAGEVPVAFVVKLKNSEATEDEIKQFISKQVVFYKKINRVFFIDAIPKSPSGKILR 535
             DE+AGEVP+AFVV+  NS  TE +I++FI+KQV  YK++ RV FI  +PKS +GKILR
Sbjct: 489 FPDEEAGEVPIAFVVRSPNSSITEQDIQKFIAKQVAPYKRLRRVSFISLVPKSAAGKILR 536

[Close]
BLAST of MELO3C024886.2 vs. Swiss-Prot
Match: sp|O24146|4CL2_TOBAC (4-coumarate--CoA ligase 2 OS=Nicotiana tabacum OX=4097 GN=4CL2 PE=1 SV=1)

HSP 1 Score: 803.9 bits (2075), Expect = 1.1e-231
Identity = 402/541 (74.31%), Postives = 459/541 (84.84%), Query Frame = 0 

Query: 4   ETVENDVIFRSKLPDIYIPNHLPLHSYCLQENAAKIGHRTCLINGVTGESFTFNDVDLTA 63
           +T + D+IFRSKLPDIYIPNHLPLHSYC  EN ++   R CLING   + +T+ DV+L +
Sbjct: 4   DTKQVDIIFRSKLPDIYIPNHLPLHSYCF-ENISEFSSRPCLINGANKQIYTYADVELNS 63

Query: 64  RKVASGLNKLGITKRDVIMLLLPNSPEFVFAFLGASHLGAIMTAANPFFTAAEIAKQAKG 123
           RKVA+GL+K GI  +D IM+LLPNSPEFVFAF+GAS+LGAI T ANP FT AE+ KQAK 
Sbjct: 64  RKVAAGLHKQGIQPKDTIMILLPNSPEFVFAFIGASYLGAISTMANPLFTPAEVVKQAKA 123

Query: 124 SKAKLIITQSSYYEKLKEITEELPEVKIMTVDSPHDGCLWFGDLIKADEREIPRVEIDPD 183
           S AK+I+TQ+ +  K+K+   E  +VKI+ +DS  +GCL F  L +A+E +IP VEI PD
Sbjct: 124 SSAKIIVTQACHVNKVKDYAFE-NDVKIICIDSAPEGCLHFSVLTQANEHDIPEVEIQPD 183

Query: 184 DVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGNEDVILCVLPLFHIYSL 243
           DVVALPYSSGTTGLPKGVMLTHK LVTSVAQQVDGENPNLY  +EDV+LCVLPLFHIYSL
Sbjct: 184 DVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYIHSEDVMLCVLPLFHIYSL 243

Query: 244 NSVLLCGLRAGSTILIMPKFEIGLLLQLVEKYGVTVAPIVPPIVLAIAKSPELEKYDLSS 303
           NSVLLCGLR G+ ILIM KF+I   L+L+++Y VT+ P VPPIVLAIAKSP ++ YDLSS
Sbjct: 244 NSVLLCGLRVGAAILIMQKFDIVSFLELIQRYKVTIGPFVPPIVLAIAKSPMVDDYDLSS 303

Query: 304 IRVIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPGACG 363
           +R + SG APLGKELEDTVRAKFP A LGQGYGMTEAGPVL M LAFAKEPF +K GACG
Sbjct: 304 VRTVMSGAAPLGKELEDTVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFEIKSGACG 363

Query: 364 TVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHTGDI 423
           TVVRNAEMKIVD +TG+SLPRN  GEICIRGDQIMKGYLN+PEATA TIDK+GWL+TGDI
Sbjct: 364 TVVRNAEMKIVDPKTGNSLPRNQSGEICIRGDQIMKGYLNDPEATARTIDKEGWLYTGDI 423

Query: 424 GFIDEDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVPVA 483
           G+ID+DDELFIVDRLKELIKYKGFQVAPAELEALLL HP ISDAAVVPMKDEQAGEVPVA
Sbjct: 424 GYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLNHPNISDAAVVPMKDEQAGEVPVA 483

Query: 484 FVVKLKNSEATEDEIKQFISKQVVFYKKINRVFFIDAIPKSPSGKILRKELRAKLAAAFP 543
           FVV+   S  TEDE+K FISKQV+FYK+I RVFF+DAIPKSPSGKILRK+LRAKLAA  P
Sbjct: 484 FVVRSNGSTITEDEVKDFISKQVIFYKRIKRVFFVDAIPKSPSGKILRKDLRAKLAAGLP 542

Query: 544 N 545
           N
Sbjct: 544 N 542

[Close]
BLAST of MELO3C024886.2 vs. Swiss-Prot
Match: sp|O24145|4CL1_TOBAC (4-coumarate--CoA ligase 1 OS=Nicotiana tabacum OX=4097 GN=4CL1 PE=2 SV=1)

HSP 1 Score: 799.7 bits (2064), Expect = 2.1e-230
Identity = 395/536 (73.69%), Postives = 452/536 (84.33%), Query Frame = 0 

Query: 9   DVIFRSKLPDIYIPNHLPLHSYCLQENAAKIGHRTCLINGVTGESFTFNDVDLTARKVAS 68
           D+IFRSKLPDIYIP HLPLHSYC  EN ++   R CLING   + +T+ +V+LT RKVA 
Sbjct: 14  DLIFRSKLPDIYIPKHLPLHSYCF-ENISEFSSRPCLINGANDQIYTYAEVELTCRKVAV 73

Query: 69  GLNKLGITKRDVIMLLLPNSPEFVFAFLGASHLGAIMTAANPFFTAAEIAKQAKGSKAKL 128
           GLNKLGI ++D IM+LLPNSPEFVFAF+GAS+LGAI T ANP FT AE+ KQAK S AK+
Sbjct: 74  GLNKLGIQQKDTIMILLPNSPEFVFAFMGASYLGAISTMANPLFTPAEVVKQAKASSAKI 133

Query: 129 IITQSSYYEKLKEITEELPEVKIMTVDSPHDGCLWFGDLIKADEREIPRVEIDPDDVVAL 188
           IITQS +  K+K+   E  +VK++ +DS  +GCL F +L ++DE EIP V+I PDDVVAL
Sbjct: 134 IITQSCFVGKVKDYASE-NDVKVICIDSAPEGCLHFSELTQSDEHEIPEVKIQPDDVVAL 193

Query: 189 PYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGNEDVILCVLPLFHIYSLNSVLL 248
           PYSSGTTGLPKGVMLTHK LVTSVAQQVDGEN NLY  +EDV++CVLPLFHIYSLNS+LL
Sbjct: 194 PYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSEDVLMCVLPLFHIYSLNSILL 253

Query: 249 CGLRAGSTILIMPKFEIGLLLQLVEKYGVTVAPIVPPIVLAIAKSPELEKYDLSSIRVIK 308
           CGLR G+ ILIM KF+I   L+L++KY V++ P VPPIVLAIAKSP ++ YDLSS+R + 
Sbjct: 254 CGLRVGAAILIMQKFDIAPFLELIQKYKVSIGPFVPPIVLAIAKSPIVDSYDLSSVRTVM 313

Query: 309 SGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPGACGTVVRN 368
           SG APLGKELED VR KFP A LGQGYGMTEAGPVL M LAFAKEPF +K GACGTVVRN
Sbjct: 314 SGAAPLGKELEDAVRTKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGACGTVVRN 373

Query: 369 AEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHTGDIGFIDE 428
           AEMKIVD +TG SLPRN PGEICIRGDQIMKGYLN+PEAT  TIDK+GWLHTGDIGFIDE
Sbjct: 374 AEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATTRTIDKEGWLHTGDIGFIDE 433

Query: 429 DDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVPVAFVVKL 488
           DDELFIVDRLKELIKYKGFQVAPAE+EALLL HP ISDAAVVPMKDEQAGEVPVAFVV+ 
Sbjct: 434 DDELFIVDRLKELIKYKGFQVAPAEIEALLLNHPNISDAAVVPMKDEQAGEVPVAFVVRS 493

Query: 489 KNSEATEDEIKQFISKQVVFYKKINRVFFIDAIPKSPSGKILRKELRAKLAAAFPN 545
             S  TEDE+K FISKQV+FYK++ RVFF++ +PKSPSGKILRK+LRA+LAA  PN
Sbjct: 494 NGSAITEDEVKDFISKQVIFYKRVKRVFFVETVPKSPSGKILRKDLRARLAAGVPN 547

[Close]
BLAST of MELO3C024886.2 vs. Swiss-Prot
Match: sp|O24540|4CL_VANPL (4-coumarate--CoA ligase OS=Vanilla planifolia OX=51239 GN=4CL PE=3 SV=1)

HSP 1 Score: 796.6 bits (2056), Expect = 1.8e-229
Identity = 408/546 (74.73%), Postives = 457/546 (83.70%), Query Frame = 0 

Query: 1   MGIETVENDVIFRSKLPDIYIPNHLPLHSYCLQENAAKIGHRTCLINGVTGESFTFNDVD 60
           + IE  + D+IFRSKLPDIYIP +LPLHSYC  EN +K   R CLING T E FT+ DV+
Sbjct: 5   VAIEEQKKDIIFRSKLPDIYIPKNLPLHSYCF-ENISKFSSRPCLINGATDEIFTYADVE 64

Query: 61  LTARKVASGLNKLGITKRDVIMLLLPNSPEFVFAFLGASHLGAIMTAANPFFTAAEIAKQ 120
           L +R+V SGL+KLGI + D IM+LLPNSPEFVFAFLGAS +G+I T ANPFFT+ E+ KQ
Sbjct: 65  LISRRVGSGLSKLGIKQGDTIMILLPNSPEFVFAFLGASFIGSISTMANPFFTSTEVIKQ 124

Query: 121 AKGSKAKLIITQSSYYEKLKEITEELPEVKIMTVDSPHD-----GCLWFGDLIKADEREI 180
           AK S AKLIITQ  Y +K+K+   E   VKI+++D+          L F +L  ADE E+
Sbjct: 125 AKASNAKLIITQGCYVDKVKDYACE-NGVKIISIDTTTTADDAANILHFSELTGADENEM 184

Query: 181 PRVEIDPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGNEDVILCVL 240
           P+VEI PD VVALPYSSGTTGLPKGVMLTHK LVTSVAQQVDGENPNLY  ++DV+LCVL
Sbjct: 185 PKVEISPDGVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYMHSDDVLLCVL 244

Query: 241 PLFHIYSLNSVLLCGLRAGSTILIMPKFEIGLLLQLVEKYGVTVAPIVPPIVLAIAKSPE 300
           PLFHIYSLNSVLLCGLRAGS ILIM KFEI   L+L++KY VT+ P VPPIVLAIAKS  
Sbjct: 245 PLFHIYSLNSVLLCGLRAGSGILIMQKFEIVPFLELIQKYKVTIGPFVPPIVLAIAKSTV 304

Query: 301 LEKYDLSSIRVIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPF 360
           ++ YDLSS+R + SG APLGKELED VRAKFP A LGQGYGMTEAGPVL M LAFAKEPF
Sbjct: 305 VDNYDLSSVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPF 364

Query: 361 PMKPGACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKD 420
            +K GACGTVVRNAEMKIVD ETGSSLPRN PGEICIRGDQIMKGYLN+PEATA TIDK+
Sbjct: 365 DIKSGACGTVVRNAEMKIVDPETGSSLPRNHPGEICIRGDQIMKGYLNDPEATARTIDKE 424

Query: 421 GWLHTGDIGFIDEDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDE 480
           GWLHTGDIG+ID+DDELFIVDRLKELIKYKGFQVAPAELEALLLTHP ISDAAVVPMKDE
Sbjct: 425 GWLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPCISDAAVVPMKDE 484

Query: 481 QAGEVPVAFVVKLKNSEATEDEIKQFISKQVVFYKKINRVFFIDAIPKSPSGKILRKELR 540
            AGEVPVAFVVK      TEDEIKQFISKQV+FYK+INRVFF++AIPK+PSGKILRK+LR
Sbjct: 485 AAGEVPVAFVVKSNGHNITEDEIKQFISKQVIFYKRINRVFFVEAIPKAPSGKILRKDLR 544

Query: 541 AKLAAA 542
           A+LAAA
Sbjct: 545 ARLAAA 548

[Close]
BLAST of MELO3C024886.2 vs. Swiss-Prot
Match: sp|P14913|4CL2_PETCR (4-coumarate--CoA ligase 1 OS=Petroselinum crispum OX=4043 GN=4CL2 PE=2 SV=1)

HSP 1 Score: 791.2 bits (2042), Expect = 7.4e-228
Identity = 394/534 (73.78%), Postives = 455/534 (85.21%), Query Frame = 0 

Query: 7   ENDVIFRSKLPDIYIPNHLPLHSYCLQENAAKIGHRTCLINGVTGESFTFNDVDLTARKV 66
           + D+IFRSKLPDIYIP HLPLH+YC  EN +K+G ++CLING TGE+FT++ V+L +RKV
Sbjct: 8   KEDLIFRSKLPDIYIPKHLPLHTYCF-ENISKVGDKSCLINGATGETFTYSQVELLSRKV 67

Query: 67  ASGLNKLGITKRDVIMLLLPNSPEFVFAFLGASHLGAIMTAANPFFTAAEIAKQAKGSKA 126
           ASGLNKLGI + D IMLLLPNSPE+ FAFLGAS+ GAI T ANPFFT+AE+ KQ K S A
Sbjct: 68  ASGLNKLGIQQGDTIMLLLPNSPEYFFAFLGASYRGAISTMANPFFTSAEVIKQLKASLA 127

Query: 127 KLIITQSSYYEKLKEITEELPEVKIMTVDSPHDGCLWFGDLIKADEREIPRVEIDPDDVV 186
           KLIITQ+ Y +K+K+   E   ++I+ +D     CL F  L++ADE E+P V ID DDVV
Sbjct: 128 KLIITQACYVDKVKDYAAE-KNIQIICIDDAPQDCLHFSKLMEADESEMPEVVIDSDDVV 187

Query: 187 ALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGNEDVILCVLPLFHIYSLNSV 246
           ALPYSSGTTGLPKGVMLTHK LVTSVAQQVDG+NPNLY  +EDV++C+LPLFHIYSLN+V
Sbjct: 188 ALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYMHSEDVMICILPLFHIYSLNAV 247

Query: 247 LLCGLRAGSTILIMPKFEIGLLLQLVEKYGVTVAPIVPPIVLAIAKSPELEKYDLSSIRV 306
           L CGLRAG TILIM KF+I   L+L++KY VT+ P VPPIVLAIAKSP ++KYDLSS+R 
Sbjct: 248 LCCGLRAGVTILIMQKFDIVPFLELIQKYKVTIGPFVPPIVLAIAKSPVVDKYDLSSVRT 307

Query: 307 IKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPGACGTVV 366
           + SG APLGKELED VRAKFP A LGQGYGMTEAGPVL M LAFAKEP+ +K GACGTVV
Sbjct: 308 VMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPYEIKSGACGTVV 367

Query: 367 RNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHTGDIGFI 426
           RNAEMKIVD ET +SLPRN  GEICIRGDQIMKGYLN+PE+T  TID++GWLHTGDIGFI
Sbjct: 368 RNAEMKIVDPETNASLPRNQRGEICIRGDQIMKGYLNDPESTRTTIDEEGWLHTGDIGFI 427

Query: 427 DEDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVPVAFVV 486
           D+DDELFIVDRLKE+IKYKGFQVAPAELEALLLTHP ISDAAVVPM DE+AGEVPVAFVV
Sbjct: 428 DDDDELFIVDRLKEIIKYKGFQVAPAELEALLLTHPTISDAAVVPMIDEKAGEVPVAFVV 487

Query: 487 KLKNSEATEDEIKQFISKQVVFYKKINRVFFIDAIPKSPSGKILRKELRAKLAA 541
           +      TE+EIKQF+SKQVVFYK+I RVFF+DAIPKSPSGKILRK+LRAK+A+
Sbjct: 488 RTNGFTTTEEEIKQFVSKQVVFYKRIFRVFFVDAIPKSPSGKILRKDLRAKIAS 539

[Close]
BLAST of MELO3C024886.2 vs. Swiss-Prot
Match: sp|P14912|4CL1_PETCR (4-coumarate--CoA ligase 1 OS=Petroselinum crispum OX=4043 GN=4CL1 PE=2 SV=1)

HSP 1 Score: 789.3 bits (2037), Expect = 2.8e-227
Identity = 392/534 (73.41%), Postives = 456/534 (85.39%), Query Frame = 0 

Query: 7   ENDVIFRSKLPDIYIPNHLPLHSYCLQENAAKIGHRTCLINGVTGESFTFNDVDLTARKV 66
           + D+IFRSKLPDIYIP HLPLH+YC  EN +K+G ++CLING TGE+FT++ V+L +RKV
Sbjct: 8   KEDLIFRSKLPDIYIPKHLPLHTYCF-ENISKVGDKSCLINGATGETFTYSQVELLSRKV 67

Query: 67  ASGLNKLGITKRDVIMLLLPNSPEFVFAFLGASHLGAIMTAANPFFTAAEIAKQAKGSKA 126
           ASGLNKLGI + D IMLLLPNSPE+ FAFLGAS+ GAI T ANPFFT+AE+ KQ K S+A
Sbjct: 68  ASGLNKLGIQQGDTIMLLLPNSPEYFFAFLGASYRGAISTMANPFFTSAEVIKQLKASQA 127

Query: 127 KLIITQSSYYEKLKEITEELPEVKIMTVDSPHDGCLWFGDLIKADEREIPRVEIDPDDVV 186
           KLIITQ+ Y +K+K+   E   ++I+ +D     CL F  L++ADE E+P V I+ DDVV
Sbjct: 128 KLIITQACYVDKVKDYAAE-KNIQIICIDDAPQDCLHFSKLMEADESEMPEVVINSDDVV 187

Query: 187 ALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGNEDVILCVLPLFHIYSLNSV 246
           ALPYSSGTTGLPKGVMLTHK LVTSVAQQVDG+NPNLY  +EDV++C+LPLFHIYSLN+V
Sbjct: 188 ALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYMHSEDVMICILPLFHIYSLNAV 247

Query: 247 LLCGLRAGSTILIMPKFEIGLLLQLVEKYGVTVAPIVPPIVLAIAKSPELEKYDLSSIRV 306
           L CGLRAG TILIM KF+I   L+L++KY VT+ P VPPIVLAIAKSP ++KYDLSS+R 
Sbjct: 248 LCCGLRAGVTILIMQKFDIVPFLELIQKYKVTIGPFVPPIVLAIAKSPVVDKYDLSSVRT 307

Query: 307 IKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPGACGTVV 366
           + SG APLGKELED VRAKFP A LGQGYGMTEAGPVL M LAFAKEP+ +K GACGTVV
Sbjct: 308 VMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPYEIKSGACGTVV 367

Query: 367 RNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHTGDIGFI 426
           RNAEMKIVD ET +SLPRN  GEICIRGDQIMKGYLN+PE+T  TID++GWLHTGDIGFI
Sbjct: 368 RNAEMKIVDPETNASLPRNQRGEICIRGDQIMKGYLNDPESTRTTIDEEGWLHTGDIGFI 427

Query: 427 DEDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVPVAFVV 486
           D+DDELFIVDRLKE+IKYKGFQVAPAELEALLLTHP ISDAAVVPM DE+AGEVPVAFVV
Sbjct: 428 DDDDELFIVDRLKEIIKYKGFQVAPAELEALLLTHPTISDAAVVPMIDEKAGEVPVAFVV 487

Query: 487 KLKNSEATEDEIKQFISKQVVFYKKINRVFFIDAIPKSPSGKILRKELRAKLAA 541
           +      TE+EIKQF+SKQVVFYK+I RVFF+DAIPKSPSGKILRK+LRA++A+
Sbjct: 488 RTNGFTTTEEEIKQFVSKQVVFYKRIFRVFFVDAIPKSPSGKILRKDLRARIAS 539

[Close]
BLAST of MELO3C024886.2 vs. TrEMBL
Match: tr|A0A1S3CJP5|A0A1S3CJP5_CUCME (4-coumarate--CoA ligase 1-like OS=Cucumis melo OX=3656 GN=LOC103501216 PE=4 SV=1)

HSP 1 Score: 1004.6 bits (2596), Expect = 8.6e-290
Identity = 510/519 (98.27%), Postives = 513/519 (98.84%), Query Frame = 0 

Query: 1   MGIETVENDVIFRSKLPDIYIPNHLPLHSYCLQENAAKIGHRTCLINGVTGESFTFNDVD 60
           MGIETVENDVIFRSKLPDIYIPNHLPLHSYCLQENAAKIGHRTCLINGVTGESFTFNDVD
Sbjct: 1   MGIETVENDVIFRSKLPDIYIPNHLPLHSYCLQENAAKIGHRTCLINGVTGESFTFNDVD 60

Query: 61  LTARKVASGLNKLGITKRDVIMLLLPNSPEFVFAFLGASHLGAIMTAANPFFTAAEIAKQ 120
           LTARKVASGLNKLGITKRDVIMLLLPNSPEFVFAFLGASHLGAIMTAANPFFTAAEIAKQ
Sbjct: 61  LTARKVASGLNKLGITKRDVIMLLLPNSPEFVFAFLGASHLGAIMTAANPFFTAAEIAKQ 120

Query: 121 AKGSKAKLIITQSSYYEKLKEITEELPEVKIMTVDSPHDGCLWFGDLIKADEREIPRVEI 180
           AKGSKAKLIITQSSYYEKLKEITEELPEVKIMTVDSPHDGCLWFGDLIKADEREIPRVEI
Sbjct: 121 AKGSKAKLIITQSSYYEKLKEITEELPEVKIMTVDSPHDGCLWFGDLIKADEREIPRVEI 180

Query: 181 DPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGNEDVILCVLPLFHI 240
           DPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGNEDVILCVLPLFHI
Sbjct: 181 DPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGNEDVILCVLPLFHI 240

Query: 241 YSLNSVLLCGLRAGSTILIMPKFEIGLLLQLVEKYGVTVAPIVPPIVLAIAKSPELEKYD 300
           YSLNSVLLCGLRAGSTILIMPKFEIGLLLQLVEKYGVTVAPIVPPIVLAIAKSPELEKYD
Sbjct: 241 YSLNSVLLCGLRAGSTILIMPKFEIGLLLQLVEKYGVTVAPIVPPIVLAIAKSPELEKYD 300

Query: 301 LSSIRVIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360
           LSSIRVIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG
Sbjct: 301 LSSIRVIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360

Query: 361 ACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420
           ACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT
Sbjct: 361 ACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420

Query: 421 GDIGFIDEDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480
           GDIGFIDEDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV
Sbjct: 421 GDIGFIDEDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480

Query: 481 PVAFVVKLKNSEATEDEIKQFISKQV-VFY--KKINRVF 517
           PVAFVVKLKNSEATEDEIKQFISKQV VF+    +N +F
Sbjct: 481 PVAFVVKLKNSEATEDEIKQFISKQVSVFFYLTNLNSIF 519

[Close]
BLAST of MELO3C024886.2 vs. TrEMBL
Match: tr|A0A0A0LF00|A0A0A0LF00_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G710200 PE=4 SV=1)

HSP 1 Score: 890.2 bits (2299), Expect = 2.4e-255
Identity = 448/465 (96.34%), Postives = 461/465 (99.14%), Query Frame = 0 

Query: 82  MLLLPNSPEFVFAFLGASHLGAIMTAANPFFTAAEIAKQAKGSKAKLIITQSSYYEKLKE 141
           MLLLPNSPEFVFAFLGAS+LGAIMTAANPFFTAAEI KQAKGSK+KLIITQSSYYEK+KE
Sbjct: 1   MLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIGKQAKGSKSKLIITQSSYYEKVKE 60

Query: 142 ITEELPEVKIMTVDSPHDGCLWFGDLIKADEREIPRVEIDPDDVVALPYSSGTTGLPKGV 201
           ITEELPEVKIMTVDSP DGCLWFGDLIKADERE+PRV+IDP+DVVALPYSSGTTGLPKGV
Sbjct: 61  ITEELPEVKIMTVDSPPDGCLWFGDLIKADEREVPRVDIDPEDVVALPYSSGTTGLPKGV 120

Query: 202 MLTHKSLVTSVAQQVDGENPNLYYGNEDVILCVLPLFHIYSLNSVLLCGLRAGSTILIMP 261
           MLTHKSLVTSVAQQVDGENPNLYYG+EDVILCVLPLFHIYSLNSVLLCGLRAGSTILIMP
Sbjct: 121 MLTHKSLVTSVAQQVDGENPNLYYGHEDVILCVLPLFHIYSLNSVLLCGLRAGSTILIMP 180

Query: 262 KFEIGLLLQLVEKYGVTVAPIVPPIVLAIAKSPELEKYDLSSIRVIKSGGAPLGKELEDT 321
           KFEIGLLLQL EKYGVTVAPIVPPIVLAIAKSPELEKYDLSSIR+IKSGGAPLGKELEDT
Sbjct: 181 KFEIGLLLQLAEKYGVTVAPIVPPIVLAIAKSPELEKYDLSSIRIIKSGGAPLGKELEDT 240

Query: 322 VRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPGACGTVVRNAEMKIVDTETGSS 381
           VRAKFPKAVLGQGYGMTEAGPVLTMGLAFAK+PFPMKPGACGTVVRNAEMKIVDTETGSS
Sbjct: 241 VRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKDPFPMKPGACGTVVRNAEMKIVDTETGSS 300

Query: 382 LPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHTGDIGFIDEDDELFIVDRLKEL 441
           LPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHTGDIGFID+DDE+FIVDRLKEL
Sbjct: 301 LPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHTGDIGFIDDDDEMFIVDRLKEL 360

Query: 442 IKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVPVAFVVKLKNSEATEDEIKQF 501
           IKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVPVAFVVKLKNSEA+EDEIKQF
Sbjct: 361 IKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVPVAFVVKLKNSEASEDEIKQF 420

Query: 502 ISKQVVFYKKINRVFFIDAIPKSPSGKILRKELRAKLAAAFPNSN 547
           ISKQVVFYK+INRVFFIDAIPKSPSGKILRKELRAKLAAAFP SN
Sbjct: 421 ISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAAAFPTSN 465

[Close]
BLAST of MELO3C024886.2 vs. TrEMBL
Match: tr|A0A2K9VP19|A0A2K9VP19_MALDO (4-coumarate:coenzyme A ligase 1 OS=Malus domestica OX=3750 GN=4CL1 PE=2 SV=1)

HSP 1 Score: 845.5 bits (2183), Expect = 6.7e-242
Identity = 421/548 (76.82%), Postives = 480/548 (87.59%), Query Frame = 0 

Query: 1   MGIETVENDVIFRSKLPDIYIPNHLPLHSYCLQENAAKIGHRTCLINGVTGESFTFNDVD 60
           M IET+ ND+++RSKLPDI IP HLPLHSYCL  N      + C+I+G TG+ +TF DV+
Sbjct: 1   MAIETIPNDIVYRSKLPDIPIPKHLPLHSYCL-HNKNHSSSKPCIIDGATGDIYTFADVE 60

Query: 61  LTARKVASGLNKLGITKRDVIMLLLPNSPEFVFAFLGASHLGAIMTAANPFFTAAEIAKQ 120
           L AR+VASGLNKLGI + DVIMLLLPNSP F FAFLGAS  GA+ TAANPFFT AEI KQ
Sbjct: 61  LNARRVASGLNKLGIQQGDVIMLLLPNSPAFAFAFLGASFRGAMTTAANPFFTPAEILKQ 120

Query: 121 AKGSKAKLIITQSSYYEKLKEI---TEELPEVKIMTVDSPHD-GCLWFGDLIKADEREIP 180
           AK SKAKLIIT + YY+K+K++   ++++ ++K+M VDSP D  CL F +L++ADE+++P
Sbjct: 121 AKASKAKLIITLACYYDKVKDLSSSSDDVHDIKLMCVDSPPDPSCLHFSELLQADEKDMP 180

Query: 181 RVEIDPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGNEDVILCVLP 240
            V+I PDDVVALPYSSGTTGLPKGVMLTHK LVTSVAQQVDGENPNLYY  +DVILCVLP
Sbjct: 181 EVDISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYYSTDDVILCVLP 240

Query: 241 LFHIYSLNSVLLCGLRAGSTILIMPKFEIGLLLQLVEKYGVTVAPIVPPIVLAIAKSPEL 300
           LFHIYSLNSVLLCGLRAG+ IL+M KFEI  LL L++KY V++APIVPPIVLAIAK P+L
Sbjct: 241 LFHIYSLNSVLLCGLRAGAAILMMNKFEIVSLLGLIDKYKVSIAPIVPPIVLAIAKFPDL 300

Query: 301 EKYDLSSIRVIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFP 360
           +KYDLSSIRV+K GGAPLGKELEDTVRAKFP   LGQGYGMTEAGPVLTM LAFAK+PF 
Sbjct: 301 DKYDLSSIRVLKCGGAPLGKELEDTVRAKFPNVTLGQGYGMTEAGPVLTMSLAFAKQPFE 360

Query: 361 MKPGACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDG 420
           +KPG CGTVVRNAE+KIVD E+G+SLPRN PGEICIRGDQIMKGYLN+PE+T  TIDK+G
Sbjct: 361 VKPGGCGTVVRNAELKIVDPESGASLPRNQPGEICIRGDQIMKGYLNDPESTRTTIDKEG 420

Query: 421 WLHTGDIGFIDEDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQ 480
           WLHTGDIGFID+DDELFIVDRLKELIKYKGFQVAPAELEALL+THP +SDAAVVPMKDE 
Sbjct: 421 WLHTGDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLITHPSVSDAAVVPMKDEA 480

Query: 481 AGEVPVAFVVKLKNSEATEDEIKQFISKQVVFYKKINRVFFIDAIPKSPSGKILRKELRA 540
           AGEVPVAFVV+  NS+ TEDE+KQFISKQVVFYK+INRVFFI+AIPKSPSGKILRK+LRA
Sbjct: 481 AGEVPVAFVVRSNNSQLTEDEVKQFISKQVVFYKRINRVFFIEAIPKSPSGKILRKDLRA 540

Query: 541 KLAAAFPN 545
           KLAA FPN
Sbjct: 541 KLAAGFPN 547

[Close]
BLAST of MELO3C024886.2 vs. TrEMBL
Match: tr|D5LLN7|D5LLN7_SORAU (p-coumarate:CoA ligase 1 OS=Sorbus aucuparia OX=36599 PE=2 SV=1)

HSP 1 Score: 844.3 bits (2180), Expect = 1.5e-241
Identity = 420/548 (76.64%), Postives = 479/548 (87.41%), Query Frame = 0 

Query: 1   MGIETVENDVIFRSKLPDIYIPNHLPLHSYCLQENAAKIGHRTCLINGVTGESFTFNDVD 60
           M IET+ ND+++RSKLPDI IP HLPLHSYCL  N      + C+I+G TG+ +TF DV+
Sbjct: 1   MAIETIPNDIVYRSKLPDIPIPKHLPLHSYCL-HNKNHSSSKPCIIDGATGDIYTFADVE 60

Query: 61  LTARKVASGLNKLGITKRDVIMLLLPNSPEFVFAFLGASHLGAIMTAANPFFTAAEIAKQ 120
           L AR+VASGLNKLGI + DVIMLLLPNSP F FAFLGAS  GA+ TAANPFFT AEI KQ
Sbjct: 61  LNARRVASGLNKLGIQQGDVIMLLLPNSPAFAFAFLGASFRGAMTTAANPFFTPAEILKQ 120

Query: 121 AKGSKAKLIITQSSYYEKLKEI---TEELPEVKIMTVDSPHD-GCLWFGDLIKADEREIP 180
           AK SKAKLIIT + YY+K+K++   ++++ ++K+M VDSP D  CL F +L++ADE ++P
Sbjct: 121 AKASKAKLIITLACYYDKVKDLSSSSDDVHDIKLMCVDSPPDPSCLHFSELLQADENDMP 180

Query: 181 RVEIDPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGNEDVILCVLP 240
            V+I PDDVVALPYSSGTTGLPKGVMLTHK LVTSVAQQVDGENPNLYY  +DV+LCVLP
Sbjct: 181 EVDISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYYSTDDVVLCVLP 240

Query: 241 LFHIYSLNSVLLCGLRAGSTILIMPKFEIGLLLQLVEKYGVTVAPIVPPIVLAIAKSPEL 300
           LFHIYSLNSVLLCGLRAG+ IL+M KFEI  LL L++KY V++APIVPPIVLAIAK P+L
Sbjct: 241 LFHIYSLNSVLLCGLRAGAAILMMNKFEIVSLLGLIDKYKVSIAPIVPPIVLAIAKFPDL 300

Query: 301 EKYDLSSIRVIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFP 360
           +KYDLSSIRV+K GGAPLGKELEDTVRAKFP   LGQGYGMTEAGPVLTM LAFAK+PF 
Sbjct: 301 DKYDLSSIRVLKCGGAPLGKELEDTVRAKFPNVTLGQGYGMTEAGPVLTMSLAFAKQPFE 360

Query: 361 MKPGACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDG 420
           +KPG CGTVVRNAE+KIVD E+G+SLPRN PGEICIRGDQIMKGYLN+PE+T  TIDK+G
Sbjct: 361 VKPGGCGTVVRNAELKIVDPESGASLPRNQPGEICIRGDQIMKGYLNDPESTRTTIDKEG 420

Query: 421 WLHTGDIGFIDEDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQ 480
           WLHTGDIGFID+DDELFIVDRLKELIKYKGFQVAPAELEALL+THP +SDAAVVPMKDE 
Sbjct: 421 WLHTGDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLITHPSVSDAAVVPMKDEA 480

Query: 481 AGEVPVAFVVKLKNSEATEDEIKQFISKQVVFYKKINRVFFIDAIPKSPSGKILRKELRA 540
           AGEVPVAFVV+  NS+ TEDE+KQFISKQVVFYK+INRVFFI+AIPKSPSGKILRK+LRA
Sbjct: 481 AGEVPVAFVVRSNNSQLTEDEVKQFISKQVVFYKRINRVFFIEAIPKSPSGKILRKDLRA 540

Query: 541 KLAAAFPN 545
           KLAA FPN
Sbjct: 541 KLAAGFPN 547

[Close]
BLAST of MELO3C024886.2 vs. TrEMBL
Match: tr|A0A2N9FA69|A0A2N9FA69_FAGSY (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS11546 PE=4 SV=1)

HSP 1 Score: 842.8 bits (2176), Expect = 4.3e-241
Identity = 423/542 (78.04%), Postives = 471/542 (86.90%), Query Frame = 0 

Query: 1   MGIETVENDVIFRSKLPDIYIPNHLPLHSYCLQENAAKIGHRTCLINGVTGESFTFNDVD 60
           M ++T + + IFRSKLPDIYIP HLPLHSYC  EN + +G R CLING TGE  T+ DV+
Sbjct: 1   MALQTKQEEFIFRSKLPDIYIPKHLPLHSYCF-ENISNVGSRPCLINGSTGEVHTYYDVE 60

Query: 61  LTARKVASGLNKLGITKRDVIMLLLPNSPEFVFAFLGASHLGAIMTAANPFFTAAEIAKQ 120
           L ARKVASGLN+LGI K DV++LLLPN+PEFVF FLGASH+GAI TAANPFFT AEIAKQ
Sbjct: 61  LKARKVASGLNQLGIGKADVVLLLLPNTPEFVFVFLGASHIGAITTAANPFFTPAEIAKQ 120

Query: 121 AKGSKAKLIITQSSYYEKLKEITEELPEVKIMTVDSPHDGCLWFGDLIKADEREIPRVEI 180
           AKGS AKLIITQ+SYY+K+K++  E   VKI+ +DSP   CL F +L KADE +IP+V I
Sbjct: 121 AKGSNAKLIITQASYYDKVKDLASENNGVKIICIDSPPQDCLHFSELTKADEHDIPQVNI 180

Query: 181 DPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGNEDVILCVLPLFHI 240
            PDDVVALPYSSGTTGLPKGVMLTHK LVTSVAQQVDGENPNLY+ +EDVILCVLPLFHI
Sbjct: 181 SPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSEDVILCVLPLFHI 240

Query: 241 YSLNSVLLCGLRAGSTILIMPKFEIGLLLQLVEKYGVTVAPIVPPIVLAIAKSPELEKYD 300
           YSLNSVLLCGLR G+ ILIM KFEI  LL+L++K+ V+V PIVPPIVLAI+KSP+L KYD
Sbjct: 241 YSLNSVLLCGLRVGAAILIMQKFEIVSLLELIQKHKVSVMPIVPPIVLAISKSPDLHKYD 300

Query: 301 LSSIRVIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360
           LSSIR++KSGGAPLGKELE+TV AKFP A  GQGYGMTEAGPVL M LAFAK PF +K G
Sbjct: 301 LSSIRILKSGGAPLGKELEETVGAKFPNAKFGQGYGMTEAGPVLAMCLAFAKTPFDVKSG 360

Query: 361 ACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420
           ACGTVVRNAEMKIVD ETG+SLPRN PGEICIRGDQIMKGY+N+P ATAATIDK+GWLHT
Sbjct: 361 ACGTVVRNAEMKIVDPETGASLPRNKPGEICIRGDQIMKGYINDPNATAATIDKEGWLHT 420

Query: 421 GDIGFIDEDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480
           GDIG ID DDELFIVDRLKELIKYKGFQVAPAELEALLLTHP ISDAAVV MKD+QAGEV
Sbjct: 421 GDIGLIDVDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPNISDAAVVSMKDDQAGEV 480

Query: 481 PVAFVVKLKNSEATEDEIKQFISKQVVFYKKINRVFFIDAIPKSPSGKILRKELRAKLAA 540
           PVAFVV+   S  TEDE+KQF+SKQVVFYK+INRVFFI+AIPK+PSGKILRK LR KLAA
Sbjct: 481 PVAFVVRSNGSNITEDEVKQFVSKQVVFYKRINRVFFIEAIPKAPSGKILRKNLREKLAA 540

Query: 541 AF 543
            F
Sbjct: 541 DF 541

The following BLAST results are available for this feature:
BLAST of MELO3C024886.2 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_004137646.21.6e-30396.34PREDICTED: 4-coumarate--CoA ligase 1 [Cucumis sativus][more]
XP_008462955.11.3e-28998.27PREDICTED: 4-coumarate--CoA ligase 1-like [Cucumis melo][more]
XP_022935389.19.0e-28389.744-coumarate--CoA ligase 1-like [Cucurbita moschata][more]
XP_023526871.16.5e-28189.194-coumarate--CoA ligase 1-like isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_022983944.13.2e-28089.194-coumarate--CoA ligase 1-like [Cucurbita maxima][more]
BLAST of MELO3C024886.2 vs. TAIR10
Match NameE-valueIdentityDescription
AT3G21240.18.3e-22270.614-coumarate:CoA ligase 2[more]
AT1G51680.15.0e-21971.304-coumarate:CoA ligase 1[more]
AT1G65060.11.1e-19764.414-coumarate:CoA ligase 3[more]
AT3G21230.11.2e-19360.734-coumarate:CoA ligase 5[more]
AT4G05160.11.7e-11843.65AMP-dependent synthetase and ligase family protein[more]
BLAST of MELO3C024886.2 vs. Swiss-Prot
Match NameE-valueIdentityDescription
sp|O24146|4CL2_TOBAC1.1e-23174.314-coumarate--CoA ligase 2 OS=Nicotiana tabacum OX=4097 GN=4CL2 PE=1 SV=1[more]
sp|O24145|4CL1_TOBAC2.1e-23073.694-coumarate--CoA ligase 1 OS=Nicotiana tabacum OX=4097 GN=4CL1 PE=2 SV=1[more]
sp|O24540|4CL_VANPL1.8e-22974.734-coumarate--CoA ligase OS=Vanilla planifolia OX=51239 GN=4CL PE=3 SV=1[more]
sp|P14913|4CL2_PETCR7.4e-22873.784-coumarate--CoA ligase 1 OS=Petroselinum crispum OX=4043 GN=4CL2 PE=2 SV=1[more]
sp|P14912|4CL1_PETCR2.8e-22773.414-coumarate--CoA ligase 1 OS=Petroselinum crispum OX=4043 GN=4CL1 PE=2 SV=1[more]
BLAST of MELO3C024886.2 vs. TrEMBL
Match NameE-valueIdentityDescription
tr|A0A1S3CJP5|A0A1S3CJP5_CUCME8.6e-29098.274-coumarate--CoA ligase 1-like OS=Cucumis melo OX=3656 GN=LOC103501216 PE=4 SV=1[more]
tr|A0A0A0LF00|A0A0A0LF00_CUCSA2.4e-25596.34Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G710200 PE=4 SV=1[more]
tr|A0A2K9VP19|A0A2K9VP19_MALDO6.7e-24276.824-coumarate:coenzyme A ligase 1 OS=Malus domestica OX=3750 GN=4CL1 PE=2 SV=1[more]
tr|D5LLN7|D5LLN7_SORAU1.5e-24176.64p-coumarate:CoA ligase 1 OS=Sorbus aucuparia OX=36599 PE=2 SV=1[more]
tr|A0A2N9FA69|A0A2N9FA69_FAGSY4.3e-24178.04Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS11546 PE=4 SV=1[more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0008152metabolic process
Vocabulary: Molecular Function
TermDefinition
GO:0003824catalytic activity
Vocabulary: INTERPRO
TermDefinition
IPR020845AMP-binding_CS
IPR025110AMP-bd_C
IPR000873AMP-dep_Synth/Lig
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008152 metabolic process
biological_process GO:0009805 coumarin biosynthetic process
biological_process GO:0009809 lignin biosynthetic process
biological_process GO:0009811 stilbene biosynthetic process
cellular_component GO:0005575 cellular_component
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0003824 catalytic activity
molecular_function GO:0016874 ligase activity
molecular_function GO:0016207 4-coumarate-CoA ligase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MELO3C024886.2.1MELO3C024886.2.1mRNA


InterPro
Analysis Name: InterPro Annotations of melon v3.6.1
Date Performed: 2018-09-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3DG3DSA:3.40.50.12780coord: 5..438
e-value: 1.3E-169
score: 566.3
NoneNo IPR availableGENE3DG3DSA:3.30.300.30coord: 439..541
e-value: 2.2E-30
score: 107.3
NoneNo IPR availablePANTHERPTHR24096:SF2074-COUMARATE--COA LIGASE 1-RELATEDcoord: 10..541
NoneNo IPR availablePANTHERPTHR24096FAMILY NOT NAMEDcoord: 10..541
NoneNo IPR availableCDDcd059044CLcoord: 21..534
e-value: 0.0
score: 790.297
NoneNo IPR availableSUPERFAMILYSSF56801Acetyl-CoA synthetase-likecoord: 17..540
IPR000873AMP-dependent synthetase/ligasePFAMPF00501AMP-bindingcoord: 32..444
e-value: 2.3E-112
score: 375.6
IPR025110AMP-binding enzyme, C-terminal domainPFAMPF13193AMP-binding_Ccoord: 453..528
e-value: 2.0E-16
score: 60.6
IPR020845AMP-binding, conserved sitePROSITEPS00455AMP_BINDINGcoord: 188..199

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
MELO3C024886.2Csa3G710200Cucumber (Chinese Long) v2cumedB178
MELO3C024886.2CSPI03G30180Wild cucumber (PI 183967)cpimedB177
MELO3C024886.2Cucsa.177420Cucumber (Gy14) v1cgymedB308
MELO3C024886.2CmoCh14G010570Cucurbita moschata (Rifu)cmomedB231
MELO3C024886.2Lsi03G012450Bottle gourd (USVL1VR-Ls)lsimedB262
MELO3C024886.2CsGy3G027980Cucumber (Gy14) v2cgybmedB158
MELO3C024886.2ClCG10G012790Watermelon (Charleston Gray)medwcgB183
MELO3C024886.2Cla97C10G195660Watermelon (97103) v2medwmbB159
MELO3C024886.2CsaV3_3G032630Cucumber (Chinese Long) v3cucmedB178
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
MELO3C024886.2Bottle gourd (USVL1VR-Ls)lsimedB196
MELO3C024886.2Watermelon (Charleston Gray)medwcgB172
MELO3C024886.2Watermelon (97103) v1medwmB197
MELO3C024886.2Silver-seed gourdcarmedB0880
MELO3C024886.2Silver-seed gourdcarmedB0897
MELO3C024886.2Cucumber (Chinese Long) v3cucmedB528
MELO3C024886.2Watermelon (97103) v2medwmbB211
MELO3C024886.2Wax gourdmedwgoB256
MELO3C024886.2Cucumber (Gy14) v2cgybmedB457
MELO3C024886.2Cucumber (Gy14) v1cgymedB467
MELO3C024886.2Cucurbita maxima (Rimu)cmamedB019
MELO3C024886.2Cucurbita maxima (Rimu)cmamedB455
MELO3C024886.2Cucurbita moschata (Rifu)cmomedB007
MELO3C024886.2Cucurbita moschata (Rifu)cmomedB443
MELO3C024886.2Cucurbita pepo (Zucchini)cpemedB595
MELO3C024886.2Cucurbita pepo (Zucchini)cpemedB847
MELO3C024886.2Wild cucumber (PI 183967)cpimedB178
MELO3C024886.2Cucumber (Chinese Long) v2cumedB179