MELO3C014533T3 (mRNA) Melon (DHL92) v3.5.1

NameMELO3C014533T3
TypemRNA
OrganismCucumis melo (Melon (DHL92) v3.5.1)
DescriptionCysteine desulfurase family protein
Locationchr5 : 1564259 .. 1568189 (-)
Sequence length1761
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
GAAGCTTTTTCGATTCTCAAAAACCATAGAGGATGATGGAAGGTATGGTTCTGAAGCTCCCATCCTTCCATTCCCAATTTCAATTCCAGCTCATGAACCCTAATCGTTGTATTTCTTGTTTCTCTTCCACCCTTCGTGCTTCCTCTTCTCCTCTTCTCCGCTTCGGTAATCCCAGATTTTCCTCTCCCTTGTCAGTTTCTGCTTCTGCTTCCACCGCCAAGGAAACCGACAGCTTGCATCTCAACTCTCTTTCGCTTGGCCACACAACTCGCCCCGATTTCCCCATCCTCCATCAGGTTCTTGTTTCACTTTTTATTCTCTTGTTTTTTTTTTTCCTTTGCTAATTTGGCCGATTTTTATGTTAACCCATTTGTCGTCTGTTTCGCTGTTATTGGCACTTCATGTGGTGGTGTTAATGGCTGTATTGCTATCTGCAGGAAATAAATGGCTCCAAGCTTGTCTACTTAGACAACGCTGCCACTTCTCAGAAACCAATTTCTGTGTTGAATGCTTTGCAGAATTACTATGAGGCTTACAACTCAAATGTGCATCGTGGCATACACTCTTTGAGGTAGGTTATTTAGACTTTGGTATGAAACGTGAATGAAAACGACTTCTATCTGATTTGAAACCGTTGGCAAATGTAGTGCGAAAGCAACTGATGAGTATGAGCGGGCAAGGAAGAAAGTTGCATCCTTTATCAATGCTGGAGATGCTAAAGAGATCATTTTTACCAGGAATGCAACTGAAGCTATCAATCTAGTGGCTTATTCTTGGGGCCTAGCGAATCTGAAATCGGAAGATGAGGTGCGTCGCCACTGCATCGATCTTAGTAGTTTTGCACAAGTGATTTTCTTCTATATATGTACTATTTAGTGCACGTATAAGCACTGACTCTGTGAGACATGGTATTCTCCATATGGGGTTAAATTGCAAATTTAATTCCAATGGTCGGAAGAAAGTTAGAATTTAGTCCTATGATTGGGACTATTTATGAAATGTTTATGAAATCATAGGGTAGTTTATCAAACCGTCAGGACTATTAGCTGTCTCTCTCATTTCTAATCTGTAAATTTCAATGGGTTTACATTTTATACTTGTTTGTATTCTTGTTGATTGTCTAAGGTGTAGGCTTCAGGGTTCTCTTTGTGAAGATTCTGATGTCAGATTTAATTAAGTTAATAGAAGTGTTTTCCATTCCTACATCATGAAGTTGCATCCAGTATTTTTTTAAATGTGTAGCCTCATACTGCATTAACTTTAAGTTTTAACTGTAACAATCTTTTCTTTCAGTGCAGATCATACTTACGGTTGCTGAACATCATAGTGCCATTGTTCCTTGGCAACTTGTAGCTGAAAGGACCGGTGCTGTTCTGAAGTTTGTAAGTTTAGGTGAACATGATGTCCCAAACTTAAAGGACTTCAAGGAAATGTTTTCAACAAAAACGAAGCTTGTGGTTACCCATCATGTCTCAAATGTACTAGGTACTTCCTTCTATTTGATATTTAGCATTTTTATAATCTATTTCCTTTTTGTACCATTCAATTTGTAAACATTACTCAAATAAAACAAGTAGTAGGTGAATATTTCAAACTGAAGTGTTATCATATTACTTCAATTATCACACTTCTAGTGGAAAGGTGTGCAGCTGGATACAAATAATAAAGTCCAATGAATGAAGAAGTGAAAGAACTGTTGGACATTTTTCCTTCTCTTGATCTGTGTCCGGTTTCTCTTGATATCTTTCTAATAATAAGTCTATGCACATAGTGGAATTCGGTATGATTTGATTGTTTTTCTGCAGCTTCTGTTCTTCCAATTGAAGAAATAGTTGGCTTGGCACATCGTTTTGGGGCAAAAGTGCTCGTAGATGCATGTCAGAGTGTTCCACATATGGTTGTCAACGTCCAGGCGCTTGATGCTGACTTTCTTGTTGCTTCTTCTCACAAGGTTAGGCTGATTTCTTATTAATTCAATGGGTAGCCTTACATTTGCGCATGTGAAGTTGAGGAATAAACATGCTTCAGATGTGTGGGCCTACAGGCATCGGATTCTTGTATGGAAAGATTGACCTGCTGTCTGCAATGCCTCCATTCTTAGGTATATCCTTCGAGAAGATAAGTTATGAAAATAAAATTGCATCAGCTCGATTTTAGGAAGTAAGTATCTAATTTTCATATTTGGTCCTACATGGCTCTAGGTGGTGGAGAGATGATCTCAGATGTATTTCTTGACCATTCCACATTTGCACAACCACCTTCCAGGTAGTGGACTGCATGGGTGCAATTTTGTCCTTTTCTTTTTACTTTGAAATGAAAATTTTCTCAATGTATTTATTTTATATGATGCGGAAAAGTTAGAACGTTCAATATATAGGGCTCAAGAATTGCAATAGAGCTAAATAACTGCTGTTTATTTAGTATTATTTGTTTGCTCATTTGTTCTCTTAGCAATTGACACTTGATAAATCATCTTTTTGGGGTCATAATGAGATCTAAATAAATGACTTCTTGAATGGTGCTAAAATCTTGTGATGGCTATTTTATTTTAACTATTCATTAATTGATGCCCTTTTACATATCATTTATGTCTTCTATGTAATCTCTTCCTGTCCAGATGCCTTTTGATCTTTGCATTAAAAAATCTTTTTGGTGGACTATTTTTTGTCCTTAATTCTACTAATTACTCATTTCCCCCTTCATTAGATTTGAAGCTGGGACGCCGGCGATTGGAGAGGCAATTGGTTTGGGAGCTGCTATTGATTATTTATCTGGGATCGGAATGACAAAAATTCATAGTTATGAAGTGAGTTGACTGAAATTTGGTATATAATAACCCTTTGTTTGAGAATTTTTGGGCGGTGAAAAAATGTGTGAAGCAACTAAATCAGGGTATCAACTAAATACCTTTATAGTAGATTAGTAATGGAATTTTCTTTAATCAAGGAATTTAGGTATTATTTTTCAATCAACTAGGTTACTTGGAACCCTGAGAAATATAGAATGAGATGATAAATATGTTTACGAGTTTTGAGACTTTAGTCTAACGACGAGGAGGTGCTGTCTGCTAACAGGAAGAATTGGCAAATTATCTATATGAAAACCTTCGTGCGGTCCCTAACATTCGGATATATGGGCCAGCTCCCACAGCACATGTTCAACGGGCTGCGCTGTGTTCTTTCAATATTGGAGACATTCATCCAACAGACATTGCAACATTTCTCGATCAACAGGTATGTTTTGGACGTTTGTCCCTATGAAATACTTTTGATGTAAGATTCCAATCCTTGAACAAAATCTTTAAGAGCCAAGGAACCAAAATCAAATAATGATGTAAGTTTCGAACTAAAGTAGAATTTAATTTAAATCCTTTCATTAACCTTTTCAGTCTCAAGGACTTGGGCTAATATCATTAGTTCATTACTGACTTATAGCTAGAGTGAATCATCTACTTTCTATAGATCGGATTCTTAAAATATTTTAATGCAGCATGGTGTAGCCATCAGATCAGGACATCACTGTGCGCAGCCCCTCCATCGGGCTCTTGGTGTGAACTCAAGTGCTCGAGTTAGCCTTTACTTCTACAACACAAAAGAGGATGTGGACTACTTTATCCAGGCTCTAAACGACACCGTAAGTTTCTTCGACTCTTTCAAGTAGAGAGATCCAGTAATACCGAACTCCTTTGTTTGACGGTCCACAGCTCGTACTTAACCTTCTGGACCACAAGGATTATGATATGTTTCTCGCTTTGAAGAAGATTGTCTGACTTTATCTTTCAGTCACATTTACTACCAGATCCTTGTGCATATAGTCACATTCTCATAGTTTTTTAGACTTAAGTGTATTTATAAATTATTTGATTAAGCAATGGTTTCATGGACTACATTTTATCTCCATAATATCAATGTACATCGACAGTTACCTGCCT

mRNA sequence

GAAGCTTTTTCGATTCTCAAAAACCATAGAGGATGATGGAAGGTATGGTTCTGAAGCTCCCATCCTTCCATTCCCAATTTCAATTCCAGCTCATGAACCCTAATCGTTGTATTTCTTGTTTCTCTTCCACCCTTCGTGCTTCCTCTTCTCCTCTTCTCCGCTTCGGTAATCCCAGATTTTCCTCTCCCTTGTCAGTTTCTGCTTCTGCTTCCACCGCCAAGGAAACCGACAGCTTGCATCTCAACTCTCTTTCGCTTGGCCACACAACTCGCCCCGATTTCCCCATCCTCCATCAGGAAATAAATGGCTCCAAGCTTGTCTACTTAGACAACGCTGCCACTTCTCAGAAACCAATTTCTGTGTTGAATGCTTTGCAGAATTACTATGAGGCTTACAACTCAAATGTGCATCGTGGCATACACTCTTTGAGTGCGAAAGCAACTGATGAGTATGAGCGGGCAAGGAAGAAAGTTGCATCCTTTATCAATGCTGGAGATGCTAAAGAGATCATTTTTACCAGGAATGCAACTGAAGCTATCAATCTAGTGGCTTATTCTTGGGGCCTAGCGAATCTGAAATCGGAAGATGAGATCATACTTACGGTTGCTGAACATCATAGTGCCATTGTTCCTTGGCAACTTGTAGCTGAAAGGACCGGTGCTGTTCTGAAGTTTGTAAGTTTAGGTGAACATGATGTCCCAAACTTAAAGGACTTCAAGGAAATGTTTTCAACAAAAACGAAGCTTGTGGTTACCCATCATGTCTCAAATGTACTAGCTTCTGTTCTTCCAATTGAAGAAATAGTTGGCTTGGCACATCGTTTTGGGGCAAAAGTGCTCGTAGATGCATGTCAGAGTGTTCCACATATGGTTGTCAACGTCCAGGCGCTTGATGCTGACTTTCTTGTTGCTTCTTCTCACAAGATGTGTGGGCCTACAGGCATCGGATTCTTGTATGGAAAGATTGACCTGCTGTCTGCAATGCCTCCATTCTTAGGTGGTGGAGAGATGATCTCAGATGTATTTCTTGACCATTCCACATTTGCACAACCACCTTCCAGATTTGAAGCTGGGACGCCGGCGATTGGAGAGGCAATTGGTTTGGGAGCTGCTATTGATTATTTATCTGGGATCGGAATGACAAAAATTCATAGTTATGAAGAAGAATTGGCAAATTATCTATATGAAAACCTTCGTGCGGTCCCTAACATTCGGATATATGGGCCAGCTCCCACAGCACATGTTCAACGGGCTGCGCTGTGTTCTTTCAATATTGGAGACATTCATCCAACAGACATTGCAACATTTCTCGATCAACAGCATGGTGTAGCCATCAGATCAGGACATCACTGTGCGCAGCCCCTCCATCGGGCTCTTGGTGTGAACTCAAGTGCTCGAGTTAGCCTTTACTTCTACAACACAAAAGAGGATGTGGACTACTTTATCCAGGCTCTAAACGACACCGTAAGTTTCTTCGACTCTTTCAAGTAGAGAGATCCAGTAATACCGAACTCCTTTGTTTGACGGTCCACAGCTCGTACTTAACCTTCTGGACCACAAGGATTATGATATGTTTCTCGCTTTGAAGAAGATTGTCTGACTTTATCTTTCAGTCACATTTACTACCAGATCCTTGTGCATATAGTCACATTCTCATAGTTTTTTAGACTTAAGTGTATTTATAAATTATTTGATTAAGCAATGGTTTCATGGACTACATTTTATCTCCATAATATCAATGTACATCGACAGTTACCTGCCT

Coding sequence (CDS)

ATGATGGAAGGTATGGTTCTGAAGCTCCCATCCTTCCATTCCCAATTTCAATTCCAGCTCATGAACCCTAATCGTTGTATTTCTTGTTTCTCTTCCACCCTTCGTGCTTCCTCTTCTCCTCTTCTCCGCTTCGGTAATCCCAGATTTTCCTCTCCCTTGTCAGTTTCTGCTTCTGCTTCCACCGCCAAGGAAACCGACAGCTTGCATCTCAACTCTCTTTCGCTTGGCCACACAACTCGCCCCGATTTCCCCATCCTCCATCAGGAAATAAATGGCTCCAAGCTTGTCTACTTAGACAACGCTGCCACTTCTCAGAAACCAATTTCTGTGTTGAATGCTTTGCAGAATTACTATGAGGCTTACAACTCAAATGTGCATCGTGGCATACACTCTTTGAGTGCGAAAGCAACTGATGAGTATGAGCGGGCAAGGAAGAAAGTTGCATCCTTTATCAATGCTGGAGATGCTAAAGAGATCATTTTTACCAGGAATGCAACTGAAGCTATCAATCTAGTGGCTTATTCTTGGGGCCTAGCGAATCTGAAATCGGAAGATGAGATCATACTTACGGTTGCTGAACATCATAGTGCCATTGTTCCTTGGCAACTTGTAGCTGAAAGGACCGGTGCTGTTCTGAAGTTTGTAAGTTTAGGTGAACATGATGTCCCAAACTTAAAGGACTTCAAGGAAATGTTTTCAACAAAAACGAAGCTTGTGGTTACCCATCATGTCTCAAATGTACTAGCTTCTGTTCTTCCAATTGAAGAAATAGTTGGCTTGGCACATCGTTTTGGGGCAAAAGTGCTCGTAGATGCATGTCAGAGTGTTCCACATATGGTTGTCAACGTCCAGGCGCTTGATGCTGACTTTCTTGTTGCTTCTTCTCACAAGATGTGTGGGCCTACAGGCATCGGATTCTTGTATGGAAAGATTGACCTGCTGTCTGCAATGCCTCCATTCTTAGGTGGTGGAGAGATGATCTCAGATGTATTTCTTGACCATTCCACATTTGCACAACCACCTTCCAGATTTGAAGCTGGGACGCCGGCGATTGGAGAGGCAATTGGTTTGGGAGCTGCTATTGATTATTTATCTGGGATCGGAATGACAAAAATTCATAGTTATGAAGAAGAATTGGCAAATTATCTATATGAAAACCTTCGTGCGGTCCCTAACATTCGGATATATGGGCCAGCTCCCACAGCACATGTTCAACGGGCTGCGCTGTGTTCTTTCAATATTGGAGACATTCATCCAACAGACATTGCAACATTTCTCGATCAACAGCATGGTGTAGCCATCAGATCAGGACATCACTGTGCGCAGCCCCTCCATCGGGCTCTTGGTGTGAACTCAAGTGCTCGAGTTAGCCTTTACTTCTACAACACAAAAGAGGATGTGGACTACTTTATCCAGGCTCTAAACGACACCGTAAGTTTCTTCGACTCTTTCAAGTAG

Protein sequence

MMEGMVLKLPSFHSQFQFQLMNPNRCISCFSSTLRASSSPLLRFGNPRFSSPLSVSASASTAKETDSLHLNSLSLGHTTRPDFPILHQEINGSKLVYLDNAATSQKPISVLNALQNYYEAYNSNVHRGIHSLSAKATDEYERARKKVASFINAGDAKEIIFTRNATEAINLVAYSWGLANLKSEDEIILTVAEHHSAIVPWQLVAERTGAVLKFVSLGEHDVPNLKDFKEMFSTKTKLVVTHHVSNVLASVLPIEEIVGLAHRFGAKVLVDACQSVPHMVVNVQALDADFLVASSHKMCGPTGIGFLYGKIDLLSAMPPFLGGGEMISDVFLDHSTFAQPPSRFEAGTPAIGEAIGLGAAIDYLSGIGMTKIHSYEEELANYLYENLRAVPNIRIYGPAPTAHVQRAALCSFNIGDIHPTDIATFLDQQHGVAIRSGHHCAQPLHRALGVNSSARVSLYFYNTKEDVDYFIQALNDTVSFFDSFK*
BLAST of MELO3C014533T3 vs. Swiss-Prot
Match: CNIF1_ARATH (Cysteine desulfurase 1, chloroplastic OS=Arabidopsis thaliana GN=NFS2 PE=1 SV=1)

HSP 1 Score: 682.2 bits (1759), Expect = 4.2e-195
Identity = 347/484 (71.69%), Postives = 390/484 (80.58%), Query Frame = 1

Query: 2   MEGMVLKLPSFHSQFQFQLMNPNRCISCFSSTLRASSSPLLRFGNPRFSSPLSVSASAST 61
           MEG+ +KLPSF          PN           A S     F   R SS LSV ++A+ 
Sbjct: 1   MEGVAMKLPSF----------PN-----------AISIGHRSFSRVRCSSSLSVCSAAAA 60

Query: 62  AKETDSLHLNSLSLGHTTRPDFPILHQEINGSKLVYLDNAATSQKPISVLNALQNYYEAY 121
           +  T S    S+SLGH  R DF ILHQE+NGSKLVYLD+AATSQKP +VL+ALQNYYE Y
Sbjct: 61  SSATISTDSESVSLGHRVRKDFRILHQEVNGSKLVYLDSAATSQKPAAVLDALQNYYEFY 120

Query: 122 NSNVHRGIHSLSAKATDEYERARKKVASFINAGDAKEIIFTRNATEAINLVAYSWGLANL 181
           NSNVHRGIH LSAKATDE+E ARKKVA FINA D++EI+FTRNATEAINLVAYSWGL+NL
Sbjct: 121 NSNVHRGIHYLSAKATDEFELARKKVARFINASDSREIVFTRNATEAINLVAYSWGLSNL 180

Query: 182 KSEDEIILTVAEHHSAIVPWQLVAERTGAVLKFVSLGEHDVPNLKDFKEMFSTKTKLVVT 241
           K  DE+ILTVAEHHS IVPWQ+V+++TGAVLKFV+L E +VP++   +E+ S KTKLV  
Sbjct: 181 KPGDEVILTVAEHHSCIVPWQIVSQKTGAVLKFVTLNEDEVPDINKLRELISPKTKLVAV 240

Query: 242 HHVSNVLASVLPIEEIVGLAHRFGAKVLVDACQSVPHMVVNVQALDADFLVASSHKMCGP 301
           HHVSNVLAS LPIEEIV  AH  GAKVLVDACQSVPHMVV+VQ L+ADFLVASSHKMCGP
Sbjct: 241 HHVSNVLASSLPIEEIVVWAHDVGAKVLVDACQSVPHMVVDVQKLNADFLVASSHKMCGP 300

Query: 302 TGIGFLYGKIDLLSAMPPFLGGGEMISDVFLDHSTFAQPPSRFEAGTPAIGEAIGLGAAI 361
           TGIGFLYGK DLL +MPPFLGGGEMISDVFLDHST+A+PPSRFEAGTPAIGEAI LGAA+
Sbjct: 301 TGIGFLYGKSDLLHSMPPFLGGGEMISDVFLDHSTYAEPPSRFEAGTPAIGEAIALGAAV 360

Query: 362 DYLSGIGMTKIHSYEEELANYLYENLRAVPNIRIYGPAPTAHVQRAALCSFNIGDIHPTD 421
           DYLSGIGM KIH YE E+  YLYE L ++P++RIYGP P+  V R ALCSFN+  +HPTD
Sbjct: 361 DYLSGIGMPKIHEYEVEIGKYLYEKLSSLPDVRIYGPRPSESVHRGALCSFNVEGLHPTD 420

Query: 422 IATFLDQQHGVAIRSGHHCAQPLHRALGVNSSARVSLYFYNTKEDVDYFIQALNDTVSFF 481
           +ATFLDQQHGVAIRSGHHCAQPLHR LGVN+SAR SLYFYNTK+DVD FI AL DTVSFF
Sbjct: 421 LATFLDQQHGVAIRSGHHCAQPLHRYLGVNASARASLYFYNTKDDVDAFIVALADTVSFF 463

Query: 482 DSFK 486
           +SFK
Sbjct: 481 NSFK 463

BLAST of MELO3C014533T3 vs. Swiss-Prot
Match: CSD_SYNY3 (Probable cysteine desulfurase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) GN=csd PE=1 SV=1)

HSP 1 Score: 524.2 bits (1349), Expect = 1.5e-147
Identity = 258/408 (63.24%), Postives = 307/408 (75.25%), Query Frame = 1

Query: 74  SLGHTTRPDFPILHQEINGSKLVYLDNAATSQKPISVLNALQNYYEAYNSNVHRGIHSLS 133
           SL  T R DFPIL+QEING  LVYLDNAATSQKP +VL  L +YYE  N+NVHRG H LS
Sbjct: 8   SLAATVRQDFPILNQEINGHPLVYLDNAATSQKPRAVLEKLMHYYENDNANVHRGAHQLS 67

Query: 134 AKATDEYERARKKVASFINAGDAKEIIFTRNATEAINLVAYSWGLANLKSEDEIILTVAE 193
            +ATD YE  R KVA FINA   +EI++TRNATEAINLVAYSWG+ NLK+ DEII TV E
Sbjct: 68  VRATDAYEAVRNKVAKFINARSPREIVYTRNATEAINLVAYSWGMNNLKAGDEIITTVME 127

Query: 194 HHSAIVPWQLVAERTGAVLKFVSLGEHDVPNLKDFKEMFSTKTKLVVTHHVSNVLASVLP 253
           HHS +VPWQ+VA +TGAVLKFV L E +  +L+ FK + S KTKLV   H+SN L  V P
Sbjct: 128 HHSNLVPWQMVAAKTGAVLKFVQLDEQESFDLEHFKTLLSEKTKLVTVVHISNTLGCVNP 187

Query: 254 IEEIVGLAHRFGAKVLVDACQSVPHMVVNVQALDADFLVASSHKMCGPTGIGFLYGKIDL 313
            EEI  LAH+ GAKVLVDACQS PH  ++VQ +D D+LVAS HKMC PTGIGFLYGK ++
Sbjct: 188 AEEIAQLAHQAGAKVLVDACQSAPHYPLDVQLIDCDWLVASGHKMCAPTGIGFLYGKEEI 247

Query: 314 LSAMPPFLGGGEMISDVFLDHSTFAQPPSRFEAGTPAIGEAIGLGAAIDYLSGIGMTKIH 373
           L AMPPF GGGEMI++VF DH T  + P +FEAGTPAI EAI LGAA+DYL+ +GM  IH
Sbjct: 248 LEAMPPFFGGGEMIAEVFFDHFTTGELPHKFEAGTPAIAEAIALGAAVDYLTDLGMENIH 307

Query: 374 SYEEELANYLYENLRAVPNIRIYGPAPTAHVQRAALCSFNIGDIHPTDIATFLDQQHGVA 433
           +YE EL +YL++ L  +P +R+YGP P  H  RAAL SFN+  +H +D+AT +DQ  G+A
Sbjct: 308 NYEVELTHYLWQGLGQIPQLRLYGPNP-KHGDRAALASFNVAGLHASDVATMVDQD-GIA 367

Query: 434 IRSGHHCAQPLHRALGVNSSARVSLYFYNTKEDVDYFIQALNDTVSFF 482
           IRSGHHC QPLHR    + SAR SLYFYNTKE++D F+Q+L  T+ FF
Sbjct: 368 IRSGHHCTQPLHRLFDASGSARASLYFYNTKEEIDLFLQSLQATIRFF 413

BLAST of MELO3C014533T3 vs. Swiss-Prot
Match: SUFS_BACSU (Cysteine desulfurase SufS OS=Bacillus subtilis (strain 168) GN=sufS PE=1 SV=1)

HSP 1 Score: 420.6 bits (1080), Expect = 2.3e-116
Identity = 209/402 (51.99%), Postives = 273/402 (67.91%), Query Frame = 1

Query: 80  RPDFPILHQEINGSKLVYLDNAATSQKPISVLNALQNYYEAYNSNVHRGIHSLSAKATDE 139
           R  FPILHQ++NG  LVYLD+AATSQKP +V+  L  YY  YNSNVHRG+H+L  +ATD 
Sbjct: 7   REQFPILHQQVNGHDLVYLDSAATSQKPRAVIETLDKYYNQYNSNVHRGVHTLGTRATDG 66

Query: 140 YERARKKVASFINAGDAKEIIFTRNATEAINLVAYSWGLANLKSEDEIILTVAEHHSAIV 199
           YE AR+KV  FINA    EIIFT+  T ++N+VA S+  ANLK  DE+++T  EHH+ I+
Sbjct: 67  YEGAREKVRKFINAKSMAEIIFTKGTTTSLNMVALSYARANLKPGDEVVITYMEHHANII 126

Query: 200 PWQLVAERTGAVLKFVSLGEHDVPNLKDFKEMFSTKTKLVVTHHVSNVLASVLPIEEIVG 259
           PWQ   + TGA LK++ L E    +L+D +E  ++ TK+V   HVSNVL +V PI+E+  
Sbjct: 127 PWQQAVKATGATLKYIPLQEDGTISLEDVRETVTSNTKIVAVSHVSNVLGTVNPIKEMAK 186

Query: 260 LAHRFGAKVLVDACQSVPHMVVNVQALDADFLVASSHKMCGPTGIGFLYGKIDLLSAMPP 319
           +AH  GA ++VD  QS PHM ++VQ LD DF   SSHKMCGPTG+G LYGK  LL  M P
Sbjct: 187 IAHDNGAVIVVDGAQSTPHMKIDVQDLDCDFFALSSHKMCGPTGVGVLYGKKALLENMEP 246

Query: 320 FLGGGEMISDVFLDHSTFAQPPSRFEAGTPAIGEAIGLGAAIDYLSGIGMTKIHSYEEEL 379
              GGEMI  V L  ST+ + P +FEAGTP I  AIGLGAAID+L  IG+ +I  +E +L
Sbjct: 247 AEFGGEMIDFVGLYESTWKELPWKFEAGTPIIAGAIGLGAAIDFLEEIGLDEISRHEHKL 306

Query: 380 ANYLYENLRAVPNIRIYGPAPTAHVQRAALCSFNIGDIHPTDIATFLDQQHGVAIRSGHH 439
           A Y  E  R +  + +YGP      +RA L +FN+ D+HP D+AT LD + G+A+R+GHH
Sbjct: 307 AAYALERFRQLDGVTVYGPE-----ERAGLVTFNLDDVHPHDVATVLDAE-GIAVRAGHH 366

Query: 440 CAQPLHRALGVNSSARVSLYFYNTKEDVDYFIQALNDTVSFF 482
           CAQPL + L V ++AR S Y YNT+E++D  ++AL  T  +F
Sbjct: 367 CAQPLMKWLDVTATARASFYLYNTEEEIDKLVEALQKTKEYF 402

BLAST of MELO3C014533T3 vs. Swiss-Prot
Match: CSD_BACHD (Probable cysteine desulfurase OS=Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) GN=csd PE=3 SV=2)

HSP 1 Score: 416.0 bits (1068), Expect = 5.7e-115
Identity = 214/405 (52.84%), Postives = 270/405 (66.67%), Query Frame = 1

Query: 77  HTTRPDFPILHQEINGSKLVYLDNAATSQKPISVLNALQNYYEAYNSNVHRGIHSLSAKA 136
           H  +  FP+L QE+NGS LVYLD+AATSQKPI+V+ AL +YY  YNSNVHRG+H+L   A
Sbjct: 4   HEIKKLFPVLDQEVNGSPLVYLDSAATSQKPIAVIEALDDYYRRYNSNVHRGVHTLGTLA 63

Query: 137 TDEYERARKKVASFINAGDAKEIIFTRNATEAINLVAYSWGLANLKSEDEIILTVAEHHS 196
           TD YE AR+K+  FI+A   +EIIFTR  T AINLVA S+G ANL   DEI++T  EHHS
Sbjct: 64  TDGYEGAREKIRRFIHASSTEEIIFTRGTTTAINLVAASYGRANLGEGDEIVITPMEHHS 123

Query: 197 AIVPWQLVAERTGAVLKFVSLGEHDVPNLKDFKEMFSTKTKLVVTHHVSNVLASVLPIEE 256
            I+PWQ VA+ TGA L ++ L +     ++D ++  S KTK+V   HVSNVL ++ P++E
Sbjct: 124 NIIPWQQVAKATGATLTYLPLQKDGTIKIEDVEKTISEKTKIVAIMHVSNVLGTINPVKE 183

Query: 257 IVGLAHRFGAKVLVDACQSVPHMVVNVQALDADFLVASSHKMCGPTGIGFLYGKIDLLSA 316
           I  +AHR GA +LVD  QS PHM ++VQ L  DF   S HKM GPTGIG LYGK   L  
Sbjct: 184 IAEIAHRHGAIMLVDGAQSAPHMKIDVQELGCDFFAFSGHKMAGPTGIGVLYGKKAHLEK 243

Query: 317 MPPFLGGGEMISDVFLDHSTFAQPPSRFEAGTPAIGEAIGLGAAIDYLSGIGMTKIHSYE 376
           M P   GGEMI  V L  ST+ + P +FE GTP I  AIGLGAAID+L  IG+ +I  +E
Sbjct: 244 MEPVEFGGEMIDFVGLYDSTWKELPWKFEGGTPIIAGAIGLGAAIDFLEDIGLDEIEKHE 303

Query: 377 EELANYLYENLRAVPNIRIYGPAPTAHVQRAALCSFNIGDIHPTDIATFLDQQHGVAIRS 436
            ELA Y  + L  +  + +YGP      +RA L +FNI D+HP D+AT LD   G+A+R+
Sbjct: 304 HELAQYALDRLSELEGMTVYGPQ-----KRAGLVTFNIEDVHPHDVATVLDAD-GIAVRA 363

Query: 437 GHHCAQPLHRALGVNSSARVSLYFYNTKEDVDYFIQALNDTVSFF 482
           GHHCAQPL + L V ++AR S Y YNTKED+D   + L  T  +F
Sbjct: 364 GHHCAQPLMKWLDVTATARASFYLYNTKEDIDALAKGLEKTKEYF 402

BLAST of MELO3C014533T3 vs. Swiss-Prot
Match: CSD_STAES (Probable cysteine desulfurase OS=Staphylococcus epidermidis (strain ATCC 12228) GN=csd PE=3 SV=1)

HSP 1 Score: 412.5 bits (1059), Expect = 6.3e-114
Identity = 207/400 (51.75%), Postives = 268/400 (67.00%), Query Frame = 1

Query: 82  DFPILHQEINGSKLVYLDNAATSQKPISVLNALQNYYEAYNSNVHRGIHSLSAKATDEYE 141
           DFPIL Q++N  +L YLD+ ATSQ P+ VLN L +YY+ YNSNVHRG+H+L + ATD YE
Sbjct: 14  DFPILEQKVNNKRLAYLDSTATSQTPVQVLNVLDDYYKRYNSNVHRGVHTLGSLATDGYE 73

Query: 142 RARKKVASFINAGDAKEIIFTRNATEAINLVAYSWGLANLKSEDEIILTVAEHHSAIVPW 201
            AR+ V  FINA   +EIIFTR  T +IN+VA+S+G AN+   DEI++T  EHH+ IVPW
Sbjct: 74  NARETVRRFINAKYFEEIIFTRGTTASINIVAHSYGDANISEGDEIVVTEMEHHANIVPW 133

Query: 202 QLVAERTGAVLKFVSLGEHDVPNLKDFKEMFSTKTKLVVTHHVSNVLASVLPIEEIVGLA 261
           Q +A+R  A LKF+ + +     L D K   + KTK+V   HVSNVL ++  ++ I  +A
Sbjct: 134 QQLAKRKNATLKFIPMTKDGELQLDDIKATINDKTKIVAIAHVSNVLGTINDVKTIAKIA 193

Query: 262 HRFGAKVLVDACQSVPHMVVNVQALDADFLVASSHKMCGPTGIGFLYGKIDLLSAMPPFL 321
           H  GA + VD  QS PHM +++Q +DADF   S HKM GPTGIG LYGK +LL  M P  
Sbjct: 194 HEHGAVISVDGAQSAPHMALDMQDIDADFYSFSGHKMLGPTGIGVLYGKRELLQNMEPVE 253

Query: 322 GGGEMISDVFLDHSTFAQPPSRFEAGTPAIGEAIGLGAAIDYLSGIGMTKIHSYEEELAN 381
            GG+MI  V    ST+A  P++FEAGTP I +AIGL  AI Y+  +G   IH +E+EL  
Sbjct: 254 FGGDMIDFVSKYDSTWADLPTKFEAGTPLIAQAIGLAEAIHYIENLGFNAIHQHEKELTE 313

Query: 382 YLYENLRAVPNIRIYGPAPTAHVQRAALCSFNIGDIHPTDIATFLDQQHGVAIRSGHHCA 441
           Y YE +  +  + IYGP      +RA + +FN+ DIHP D+AT +D + GVA+R+GHHCA
Sbjct: 314 YAYEQMLTIDGLEIYGPPKD---RRAGVITFNLADIHPHDVATAVDTE-GVAVRAGHHCA 373

Query: 442 QPLHRALGVNSSARVSLYFYNTKEDVDYFIQALNDTVSFF 482
           QPL + LGV+S+AR S Y YNTKEDVD  +QAL  T  FF
Sbjct: 374 QPLMKWLGVSSTARASFYVYNTKEDVDQLVQALKQTKEFF 409

BLAST of MELO3C014533T3 vs. TrEMBL
Match: A0A067KRV2_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_11109 PE=4 SV=1)

HSP 1 Score: 717.2 bits (1850), Expect = 1.3e-203
Identity = 365/489 (74.64%), Postives = 412/489 (84.25%), Query Frame = 1

Query: 2   MEGMVLKLPSFHSQFQFQLMNPNRCISCFSSTLRASSSPL-LRFGNPRFSSPLS----VS 61
           M+G V KLPSF      + + PN      SS    + SP+ +R G  R SS  S    + 
Sbjct: 1   MDGAVFKLPSF------KFLRPN------SSPPATTKSPVVIRCGLTRLSSSYSSSSAIQ 60

Query: 62  ASASTAKETDSLHLNSLSLGHTTRPDFPILHQEINGSKLVYLDNAATSQKPISVLNALQN 121
           A A+T +E D   L SLSLGH TRPDFPILHQE+NGSKLVYLDNAATSQKP SVL ALQN
Sbjct: 61  AIATTGREGDQA-LGSLSLGHITRPDFPILHQEVNGSKLVYLDNAATSQKPNSVLRALQN 120

Query: 122 YYEAYNSNVHRGIHSLSAKATDEYERARKKVASFINAGDAKEIIFTRNATEAINLVAYSW 181
           YYE YNSNVHRGIH LSAKATDEYE ARKKVA+FINA D++EI+FTRNATEAINLVAYSW
Sbjct: 121 YYETYNSNVHRGIHYLSAKATDEYELARKKVAAFINASDSREIVFTRNATEAINLVAYSW 180

Query: 182 GLANLKSEDEIILTVAEHHSAIVPWQLVAERTGAVLKFVSLGEHDVPNLKDFKEMFSTKT 241
           GLANLK  DE+++T+AEHHSAIVPWQLVA+RTGA+LKFV+L E++VP+++  KE+ S  T
Sbjct: 181 GLANLKPGDEVVVTIAEHHSAIVPWQLVAQRTGALLKFVNLNENEVPDVQQLKEVISQNT 240

Query: 242 KLVVTHHVSNVLASVLPIEEIVGLAHRFGAKVLVDACQSVPHMVVNVQALDADFLVASSH 301
           +LVV HH+SNVLAS LPIE+I+  AH  GAKVL+DACQSVPHMVV+VQ L+ADFLVASSH
Sbjct: 241 RLVVVHHISNVLASFLPIEDIIEWAHDAGAKVLIDACQSVPHMVVDVQRLNADFLVASSH 300

Query: 302 KMCGPTGIGFLYGKIDLLSAMPPFLGGGEMISDVFLDHSTFAQPPSRFEAGTPAIGEAIG 361
           KMCGPTGIGFLYGK D+LS+MPPFLGGGEMISDVFLDHST+A+PPSRFEAGTPAIGEAIG
Sbjct: 301 KMCGPTGIGFLYGKSDILSSMPPFLGGGEMISDVFLDHSTYAEPPSRFEAGTPAIGEAIG 360

Query: 362 LGAAIDYLSGIGMTKIHSYEEELANYLYENLRAVPNIRIYGPAPTAHVQRAALCSFNIGD 421
           LGAAIDYL GIGM KIH YE ELANYLYE+L +VPN+RIYGP P+  V RAALCSFN+  
Sbjct: 361 LGAAIDYLLGIGMQKIHDYEMELANYLYESLSSVPNVRIYGPKPSETVHRAALCSFNVEG 420

Query: 422 IHPTDIATFLDQQHGVAIRSGHHCAQPLHRALGVNSSARVSLYFYNTKEDVDYFIQALND 481
           IHPTDIATFLDQQHGVAIRSGHHCAQPLHR LGVN+SAR SL+FYNTKEDVD FIQALND
Sbjct: 421 IHPTDIATFLDQQHGVAIRSGHHCAQPLHRHLGVNASARASLHFYNTKEDVDDFIQALND 476

Query: 482 TVSFFDSFK 486
           T+SFF+SFK
Sbjct: 481 TISFFNSFK 476

BLAST of MELO3C014533T3 vs. TrEMBL
Match: A0A061GUT7_THECC (Chloroplastic NIFS-like cysteine desulfurase isoform 1 OS=Theobroma cacao GN=TCM_040966 PE=4 SV=1)

HSP 1 Score: 714.9 bits (1844), Expect = 6.6e-203
Identity = 369/485 (76.08%), Postives = 407/485 (83.92%), Query Frame = 1

Query: 1   MMEGMVLKLPSFHSQFQFQLMNPNRCISCFSSTLRASSSPLLRFGNPRFSSPLSVSASAS 60
           MMEG+V+KLPSF       L+NP          L +SS PL R G  RFS      AS S
Sbjct: 4   MMEGVVVKLPSF------PLLNPT------IPPLSSSSLPL-RLGFRRFS------ASLS 63

Query: 61  TAKETDSLHLNSLSLGHTTRPDFPILHQEINGSKLVYLDNAATSQKPISVLNALQNYYEA 120
             KE       S+SLGH +RPDFPILHQE+NGSKLVYLDNAATSQKP +VL AL NYYEA
Sbjct: 64  AVKE------GSVSLGHLSRPDFPILHQEVNGSKLVYLDNAATSQKPTAVLKALHNYYEA 123

Query: 121 YNSNVHRGIHSLSAKATDEYERARKKVASFINAGDAKEIIFTRNATEAINLVAYSWGLAN 180
           YNSNVHRGIH LSAKATDEYE ARKKVA+FINA D+ EI+FTRNATEAINLVAYSWGL N
Sbjct: 124 YNSNVHRGIHYLSAKATDEYELARKKVAAFINASDSSEIVFTRNATEAINLVAYSWGLLN 183

Query: 181 LKSEDEIILTVAEHHSAIVPWQLVAERTGAVLKFVSLGEHDVPNLKDFKEMFSTKTKLVV 240
           LK  DEI+LT+AEHHSAIVPWQ+VA++TGAVLKFVSL E+++PN+   +EM ST+T+LVV
Sbjct: 184 LKPGDEIVLTIAEHHSAIVPWQIVAQKTGAVLKFVSLDENEIPNVVKLREMISTRTELVV 243

Query: 241 THHVSNVLASVLPIEEIVGLAHRFGAKVLVDACQSVPHMVVNVQALDADFLVASSHKMCG 300
            HHVSNVL SVLPIEEIV   H  GAKVLVDACQSVPHMVV+VQ LDADFLVASSHKMCG
Sbjct: 244 VHHVSNVLGSVLPIEEIVHWVHEVGAKVLVDACQSVPHMVVDVQGLDADFLVASSHKMCG 303

Query: 301 PTGIGFLYGKIDLLSAMPPFLGGGEMISDVFLDHSTFAQPPSRFEAGTPAIGEAIGLGAA 360
           PTGIGFL+GK +LLSAMPPFLGGGEMISDVFLDHST+A+PPSRFEAGTPAIGEAIGLGAA
Sbjct: 304 PTGIGFLFGKSNLLSAMPPFLGGGEMISDVFLDHSTYAEPPSRFEAGTPAIGEAIGLGAA 363

Query: 361 IDYLSGIGMTKIHSYEEELANYLYENLRAVPNIRIYGPAPTAHVQRAALCSFNIGDIHPT 420
           IDYLSGIGM KIH YE ELANYLY+ L +VPNIRIYGP P   V RAALCSFN+ +IHPT
Sbjct: 364 IDYLSGIGMQKIHDYEMELANYLYDKLCSVPNIRIYGPKPAETVYRAALCSFNVENIHPT 423

Query: 421 DIATFLDQQHGVAIRSGHHCAQPLHRALGVNSSARVSLYFYNTKEDVDYFIQALNDTVSF 480
           D+ATFLDQQHGVAIRSGHHCAQPLHR LGVN+SAR SL+FYNT+EDVD FI+ALNDTVSF
Sbjct: 424 DLATFLDQQHGVAIRSGHHCAQPLHRYLGVNASARASLHFYNTEEDVDDFIRALNDTVSF 463

Query: 481 FDSFK 486
           F+SFK
Sbjct: 484 FNSFK 463

BLAST of MELO3C014533T3 vs. TrEMBL
Match: A0A0S3S493_PHAAN (Uncharacterized protein OS=Vigna angularis var. angularis GN=Vigan.05G104600 PE=3 SV=1)

HSP 1 Score: 712.6 bits (1838), Expect = 3.3e-202
Identity = 365/485 (75.26%), Postives = 411/485 (84.74%), Query Frame = 1

Query: 2   MEGMVLKLPSFHSQFQFQLMNPNRCISCFSSTLRASSSPLLRFGNPRFSSPLSVSASAST 61
           ME ++ KLPSF      +  +P+ C S    T+R+SS  ++RFG  R SS   +S  AST
Sbjct: 1   MEVLLPKLPSF------KFPSPSHCCSI---TIRSSS--IVRFGLRRVSS---LSVFAST 60

Query: 62  AKETDSLH-LNSLSLGHTTRPDFPILHQEINGSKLVYLDNAATSQKPISVLNALQNYYEA 121
             E  +   + + SLGH+TRP FPILHQE+NGSKLVYLDNAATSQKP  VL ALQNYYE 
Sbjct: 61  VNEAVAQPAIGTPSLGHSTRPHFPILHQEVNGSKLVYLDNAATSQKPTVVLKALQNYYEG 120

Query: 122 YNSNVHRGIHSLSAKATDEYERARKKVASFINAGDAKEIIFTRNATEAINLVAYSWGLAN 181
           YNSNVHRGIH LSAKATDEYE AR+KVASFINA D++EIIFT+NA+EAINLVAYSWGL+N
Sbjct: 121 YNSNVHRGIHFLSAKATDEYESARRKVASFINASDSREIIFTKNASEAINLVAYSWGLSN 180

Query: 182 LKSEDEIILTVAEHHSAIVPWQLVAERTGAVLKFVSLGEHDVPNLKDFKEMFSTKTKLVV 241
           LK EDEIILTVAEHHSAIVPWQLVAE+ GAVLKFV L +  +P++   K+MFS KTK+VV
Sbjct: 181 LKPEDEIILTVAEHHSAIVPWQLVAEKVGAVLKFVDLNQDGIPDIGKLKDMFSRKTKIVV 240

Query: 242 THHVSNVLASVLPIEEIVGLAHRFGAKVLVDACQSVPHMVVNVQALDADFLVASSHKMCG 301
            HHVSNVLASVLPI +I   AH  GAKVLVDACQSVPHMVV+VQ+L+ DFLVASSHKMCG
Sbjct: 241 VHHVSNVLASVLPIRDIAQWAHDVGAKVLVDACQSVPHMVVDVQSLNVDFLVASSHKMCG 300

Query: 302 PTGIGFLYGKIDLLSAMPPFLGGGEMISDVFLDHSTFAQPPSRFEAGTPAIGEAIGLGAA 361
           PTGIGFLYG+IDLLS+MPPFLGGGEMISDV+LDHST+A+PPSRFEAGTPAIGEAIGLGAA
Sbjct: 301 PTGIGFLYGRIDLLSSMPPFLGGGEMISDVYLDHSTYAEPPSRFEAGTPAIGEAIGLGAA 360

Query: 362 IDYLSGIGMTKIHSYEEELANYLYENLRAVPNIRIYGPAPTAHVQRAALCSFNIGDIHPT 421
           IDYLSGIGM  IH YE EL  YLYE L +VPNIRIYGPAP+ +VQRAALCSFN+ ++HPT
Sbjct: 361 IDYLSGIGMQTIHDYEVELGRYLYERLLSVPNIRIYGPAPSENVQRAALCSFNVENLHPT 420

Query: 422 DIATFLDQQHGVAIRSGHHCAQPLHRALGVNSSARVSLYFYNTKEDVDYFIQALNDTVSF 481
           D+ATFLDQQHGVAIRSGHHCAQPLHR LGV+SSAR SLYFYNTKEDVDYFI ALNDTVSF
Sbjct: 421 DLATFLDQQHGVAIRSGHHCAQPLHRFLGVSSSARASLYFYNTKEDVDYFIHALNDTVSF 471

Query: 482 FDSFK 486
           F+SFK
Sbjct: 481 FNSFK 471

BLAST of MELO3C014533T3 vs. TrEMBL
Match: A0A0L9VET2_PHAAN (Uncharacterized protein OS=Phaseolus angularis GN=LR48_Vigan09g185300 PE=3 SV=1)

HSP 1 Score: 712.6 bits (1838), Expect = 3.3e-202
Identity = 365/485 (75.26%), Postives = 411/485 (84.74%), Query Frame = 1

Query: 2   MEGMVLKLPSFHSQFQFQLMNPNRCISCFSSTLRASSSPLLRFGNPRFSSPLSVSASAST 61
           ME ++ KLPSF      +  +P+ C S    T+R+SS  ++RFG  R SS   +S  AST
Sbjct: 1   MEVLLPKLPSF------KFPSPSHCCSI---TIRSSS--IVRFGLRRVSS---LSVFAST 60

Query: 62  AKETDSLH-LNSLSLGHTTRPDFPILHQEINGSKLVYLDNAATSQKPISVLNALQNYYEA 121
             E  +   + + SLGH+TRP FPILHQE+NGSKLVYLDNAATSQKP  VL ALQNYYE 
Sbjct: 61  VNEAVAQPAIGTPSLGHSTRPHFPILHQEVNGSKLVYLDNAATSQKPTVVLKALQNYYEG 120

Query: 122 YNSNVHRGIHSLSAKATDEYERARKKVASFINAGDAKEIIFTRNATEAINLVAYSWGLAN 181
           YNSNVHRGIH LSAKATDEYE AR+KVASFINA D++EIIFT+NA+EAINLVAYSWGL+N
Sbjct: 121 YNSNVHRGIHFLSAKATDEYESARRKVASFINASDSREIIFTKNASEAINLVAYSWGLSN 180

Query: 182 LKSEDEIILTVAEHHSAIVPWQLVAERTGAVLKFVSLGEHDVPNLKDFKEMFSTKTKLVV 241
           LK EDEIILTVAEHHSAIVPWQLVAE+ GAVLKFV L +  +P++   K+MFS KTK+VV
Sbjct: 181 LKPEDEIILTVAEHHSAIVPWQLVAEKVGAVLKFVDLNQDGIPDIGKLKDMFSRKTKIVV 240

Query: 242 THHVSNVLASVLPIEEIVGLAHRFGAKVLVDACQSVPHMVVNVQALDADFLVASSHKMCG 301
            HHVSNVLASVLPI +I   AH  GAKVLVDACQSVPHMVV+VQ+L+ DFLVASSHKMCG
Sbjct: 241 VHHVSNVLASVLPIRDIAQWAHDVGAKVLVDACQSVPHMVVDVQSLNVDFLVASSHKMCG 300

Query: 302 PTGIGFLYGKIDLLSAMPPFLGGGEMISDVFLDHSTFAQPPSRFEAGTPAIGEAIGLGAA 361
           PTGIGFLYG+IDLLS+MPPFLGGGEMISDV+LDHST+A+PPSRFEAGTPAIGEAIGLGAA
Sbjct: 301 PTGIGFLYGRIDLLSSMPPFLGGGEMISDVYLDHSTYAEPPSRFEAGTPAIGEAIGLGAA 360

Query: 362 IDYLSGIGMTKIHSYEEELANYLYENLRAVPNIRIYGPAPTAHVQRAALCSFNIGDIHPT 421
           IDYLSGIGM  IH YE EL  YLYE L +VPNIRIYGPAP+ +VQRAALCSFN+ ++HPT
Sbjct: 361 IDYLSGIGMQTIHDYEVELGRYLYERLLSVPNIRIYGPAPSENVQRAALCSFNVENLHPT 420

Query: 422 DIATFLDQQHGVAIRSGHHCAQPLHRALGVNSSARVSLYFYNTKEDVDYFIQALNDTVSF 481
           D+ATFLDQQHGVAIRSGHHCAQPLHR LGV+SSAR SLYFYNTKEDVDYFI ALNDTVSF
Sbjct: 421 DLATFLDQQHGVAIRSGHHCAQPLHRFLGVSSSARASLYFYNTKEDVDYFIHALNDTVSF 471

Query: 482 FDSFK 486
           F+SFK
Sbjct: 481 FNSFK 471

BLAST of MELO3C014533T3 vs. TrEMBL
Match: V7BND5_PHAVU (Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_006G131100g PE=3 SV=1)

HSP 1 Score: 711.4 bits (1835), Expect = 7.2e-202
Identity = 362/484 (74.79%), Postives = 411/484 (84.92%), Query Frame = 1

Query: 2   MEGMVLKLPSFHSQFQFQLMNPNRCISCFSSTLRASSSPLLRFGNPRFSSPLSVSASAST 61
           ME ++ KLPSF      +  +P+ C S    T+R+SS   +RFG  R SS LSV AS   
Sbjct: 1   MEVLLPKLPSF------KFSSPSHCCSI---TIRSSS--FVRFGFRRVSS-LSVFASTVN 60

Query: 62  AKETDSLHLNSLSLGHTTRPDFPILHQEINGSKLVYLDNAATSQKPISVLNALQNYYEAY 121
               +   + S SLGH+TRP FPILHQE+NGSKLVYLDNAATSQKP +VL ALQNYYE Y
Sbjct: 61  EAVAEPA-VGSPSLGHSTRPHFPILHQEVNGSKLVYLDNAATSQKPTAVLKALQNYYEGY 120

Query: 122 NSNVHRGIHSLSAKATDEYERARKKVASFINAGDAKEIIFTRNATEAINLVAYSWGLANL 181
           NSNVHRGIH LSAKATDEYE AR+KVASFINA D++EI+FT+NA+E +NLVAYSWGL+NL
Sbjct: 121 NSNVHRGIHFLSAKATDEYESARRKVASFINASDSREIVFTKNASEGVNLVAYSWGLSNL 180

Query: 182 KSEDEIILTVAEHHSAIVPWQLVAERTGAVLKFVSLGEHDVPNLKDFKEMFSTKTKLVVT 241
           K EDEIILTVAEHHSAIVPWQL+A++ GAVLKFV L +  +P++   KEMFS KTK+VV 
Sbjct: 181 KPEDEIILTVAEHHSAIVPWQLIAKKVGAVLKFVDLNQDGIPDIDKMKEMFSRKTKIVVV 240

Query: 242 HHVSNVLASVLPIEEIVGLAHRFGAKVLVDACQSVPHMVVNVQALDADFLVASSHKMCGP 301
           HHVSNVLASVLPI +I   AH  GAKVLVDACQSVPHM+V+VQ+L+ DFLVASSHKMCGP
Sbjct: 241 HHVSNVLASVLPIRDIAQWAHDVGAKVLVDACQSVPHMMVDVQSLNVDFLVASSHKMCGP 300

Query: 302 TGIGFLYGKIDLLSAMPPFLGGGEMISDVFLDHSTFAQPPSRFEAGTPAIGEAIGLGAAI 361
           TGIGFL+GKIDLLS+MPPFLGGGEMISDV+LDHST+A+PPSRFEAGTPAIGEAIGLGAAI
Sbjct: 301 TGIGFLFGKIDLLSSMPPFLGGGEMISDVYLDHSTYAEPPSRFEAGTPAIGEAIGLGAAI 360

Query: 362 DYLSGIGMTKIHSYEEELANYLYENLRAVPNIRIYGPAPTAHVQRAALCSFNIGDIHPTD 421
           DYLSGIGM  IH YE EL+ YLYE L +VPNIRIYGPAP+  VQRAALCSFN+ ++HPTD
Sbjct: 361 DYLSGIGMQTIHDYEVELSRYLYERLVSVPNIRIYGPAPSEKVQRAALCSFNVENLHPTD 420

Query: 422 IATFLDQQHGVAIRSGHHCAQPLHRALGVNSSARVSLYFYNTKEDVDYFIQALNDTVSFF 481
           +ATFLDQQHGVAIRSGHHCAQPLHR+LGV+SSARVSLYFYNTKEDVDYFI ALNDTVSFF
Sbjct: 421 LATFLDQQHGVAIRSGHHCAQPLHRSLGVSSSARVSLYFYNTKEDVDYFIHALNDTVSFF 471

Query: 482 DSFK 486
           +SFK
Sbjct: 481 NSFK 471

BLAST of MELO3C014533T3 vs. TAIR10
Match: AT1G08490.1 (AT1G08490.1 chloroplastic NIFS-like cysteine desulfurase)

HSP 1 Score: 682.2 bits (1759), Expect = 2.4e-196
Identity = 347/484 (71.69%), Postives = 390/484 (80.58%), Query Frame = 1

Query: 2   MEGMVLKLPSFHSQFQFQLMNPNRCISCFSSTLRASSSPLLRFGNPRFSSPLSVSASAST 61
           MEG+ +KLPSF          PN           A S     F   R SS LSV ++A+ 
Sbjct: 1   MEGVAMKLPSF----------PN-----------AISIGHRSFSRVRCSSSLSVCSAAAA 60

Query: 62  AKETDSLHLNSLSLGHTTRPDFPILHQEINGSKLVYLDNAATSQKPISVLNALQNYYEAY 121
           +  T S    S+SLGH  R DF ILHQE+NGSKLVYLD+AATSQKP +VL+ALQNYYE Y
Sbjct: 61  SSATISTDSESVSLGHRVRKDFRILHQEVNGSKLVYLDSAATSQKPAAVLDALQNYYEFY 120

Query: 122 NSNVHRGIHSLSAKATDEYERARKKVASFINAGDAKEIIFTRNATEAINLVAYSWGLANL 181
           NSNVHRGIH LSAKATDE+E ARKKVA FINA D++EI+FTRNATEAINLVAYSWGL+NL
Sbjct: 121 NSNVHRGIHYLSAKATDEFELARKKVARFINASDSREIVFTRNATEAINLVAYSWGLSNL 180

Query: 182 KSEDEIILTVAEHHSAIVPWQLVAERTGAVLKFVSLGEHDVPNLKDFKEMFSTKTKLVVT 241
           K  DE+ILTVAEHHS IVPWQ+V+++TGAVLKFV+L E +VP++   +E+ S KTKLV  
Sbjct: 181 KPGDEVILTVAEHHSCIVPWQIVSQKTGAVLKFVTLNEDEVPDINKLRELISPKTKLVAV 240

Query: 242 HHVSNVLASVLPIEEIVGLAHRFGAKVLVDACQSVPHMVVNVQALDADFLVASSHKMCGP 301
           HHVSNVLAS LPIEEIV  AH  GAKVLVDACQSVPHMVV+VQ L+ADFLVASSHKMCGP
Sbjct: 241 HHVSNVLASSLPIEEIVVWAHDVGAKVLVDACQSVPHMVVDVQKLNADFLVASSHKMCGP 300

Query: 302 TGIGFLYGKIDLLSAMPPFLGGGEMISDVFLDHSTFAQPPSRFEAGTPAIGEAIGLGAAI 361
           TGIGFLYGK DLL +MPPFLGGGEMISDVFLDHST+A+PPSRFEAGTPAIGEAI LGAA+
Sbjct: 301 TGIGFLYGKSDLLHSMPPFLGGGEMISDVFLDHSTYAEPPSRFEAGTPAIGEAIALGAAV 360

Query: 362 DYLSGIGMTKIHSYEEELANYLYENLRAVPNIRIYGPAPTAHVQRAALCSFNIGDIHPTD 421
           DYLSGIGM KIH YE E+  YLYE L ++P++RIYGP P+  V R ALCSFN+  +HPTD
Sbjct: 361 DYLSGIGMPKIHEYEVEIGKYLYEKLSSLPDVRIYGPRPSESVHRGALCSFNVEGLHPTD 420

Query: 422 IATFLDQQHGVAIRSGHHCAQPLHRALGVNSSARVSLYFYNTKEDVDYFIQALNDTVSFF 481
           +ATFLDQQHGVAIRSGHHCAQPLHR LGVN+SAR SLYFYNTK+DVD FI AL DTVSFF
Sbjct: 421 LATFLDQQHGVAIRSGHHCAQPLHRYLGVNASARASLYFYNTKDDVDAFIVALADTVSFF 463

Query: 482 DSFK 486
           +SFK
Sbjct: 481 NSFK 463

BLAST of MELO3C014533T3 vs. NCBI nr
Match: gi|659097418|ref|XP_008449615.1| (PREDICTED: cysteine desulfurase 2, chloroplastic-like [Cucumis melo])

HSP 1 Score: 966.8 bits (2498), Expect = 1.4e-278
Identity = 485/485 (100.00%), Postives = 485/485 (100.00%), Query Frame = 1

Query: 1   MMEGMVLKLPSFHSQFQFQLMNPNRCISCFSSTLRASSSPLLRFGNPRFSSPLSVSASAS 60
           MMEGMVLKLPSFHSQFQFQLMNPNRCISCFSSTLRASSSPLLRFGNPRFSSPLSVSASAS
Sbjct: 1   MMEGMVLKLPSFHSQFQFQLMNPNRCISCFSSTLRASSSPLLRFGNPRFSSPLSVSASAS 60

Query: 61  TAKETDSLHLNSLSLGHTTRPDFPILHQEINGSKLVYLDNAATSQKPISVLNALQNYYEA 120
           TAKETDSLHLNSLSLGHTTRPDFPILHQEINGSKLVYLDNAATSQKPISVLNALQNYYEA
Sbjct: 61  TAKETDSLHLNSLSLGHTTRPDFPILHQEINGSKLVYLDNAATSQKPISVLNALQNYYEA 120

Query: 121 YNSNVHRGIHSLSAKATDEYERARKKVASFINAGDAKEIIFTRNATEAINLVAYSWGLAN 180
           YNSNVHRGIHSLSAKATDEYERARKKVASFINAGDAKEIIFTRNATEAINLVAYSWGLAN
Sbjct: 121 YNSNVHRGIHSLSAKATDEYERARKKVASFINAGDAKEIIFTRNATEAINLVAYSWGLAN 180

Query: 181 LKSEDEIILTVAEHHSAIVPWQLVAERTGAVLKFVSLGEHDVPNLKDFKEMFSTKTKLVV 240
           LKSEDEIILTVAEHHSAIVPWQLVAERTGAVLKFVSLGEHDVPNLKDFKEMFSTKTKLVV
Sbjct: 181 LKSEDEIILTVAEHHSAIVPWQLVAERTGAVLKFVSLGEHDVPNLKDFKEMFSTKTKLVV 240

Query: 241 THHVSNVLASVLPIEEIVGLAHRFGAKVLVDACQSVPHMVVNVQALDADFLVASSHKMCG 300
           THHVSNVLASVLPIEEIVGLAHRFGAKVLVDACQSVPHMVVNVQALDADFLVASSHKMCG
Sbjct: 241 THHVSNVLASVLPIEEIVGLAHRFGAKVLVDACQSVPHMVVNVQALDADFLVASSHKMCG 300

Query: 301 PTGIGFLYGKIDLLSAMPPFLGGGEMISDVFLDHSTFAQPPSRFEAGTPAIGEAIGLGAA 360
           PTGIGFLYGKIDLLSAMPPFLGGGEMISDVFLDHSTFAQPPSRFEAGTPAIGEAIGLGAA
Sbjct: 301 PTGIGFLYGKIDLLSAMPPFLGGGEMISDVFLDHSTFAQPPSRFEAGTPAIGEAIGLGAA 360

Query: 361 IDYLSGIGMTKIHSYEEELANYLYENLRAVPNIRIYGPAPTAHVQRAALCSFNIGDIHPT 420
           IDYLSGIGMTKIHSYEEELANYLYENLRAVPNIRIYGPAPTAHVQRAALCSFNIGDIHPT
Sbjct: 361 IDYLSGIGMTKIHSYEEELANYLYENLRAVPNIRIYGPAPTAHVQRAALCSFNIGDIHPT 420

Query: 421 DIATFLDQQHGVAIRSGHHCAQPLHRALGVNSSARVSLYFYNTKEDVDYFIQALNDTVSF 480
           DIATFLDQQHGVAIRSGHHCAQPLHRALGVNSSARVSLYFYNTKEDVDYFIQALNDTVSF
Sbjct: 421 DIATFLDQQHGVAIRSGHHCAQPLHRALGVNSSARVSLYFYNTKEDVDYFIQALNDTVSF 480

Query: 481 FDSFK 486
           FDSFK
Sbjct: 481 FDSFK 485

BLAST of MELO3C014533T3 vs. NCBI nr
Match: gi|778716806|ref|XP_004140178.2| (PREDICTED: cysteine desulfurase 1, chloroplastic [Cucumis sativus])

HSP 1 Score: 929.5 bits (2401), Expect = 2.4e-267
Identity = 466/485 (96.08%), Postives = 472/485 (97.32%), Query Frame = 1

Query: 1   MMEGMVLKLPSFHSQFQFQLMNPNRCISCFSSTLRASSSPLLRFGNPRFSSPLSVSASAS 60
           MMEGMVLK PSFHSQF FQ MNPNRCISCFSSTLRASSSPLLRF NPR  S  SVSASAS
Sbjct: 30  MMEGMVLKSPSFHSQFHFQFMNPNRCISCFSSTLRASSSPLLRFRNPRLPSHFSVSASAS 89

Query: 61  TAKETDSLHLNSLSLGHTTRPDFPILHQEINGSKLVYLDNAATSQKPISVLNALQNYYEA 120
           TAKETDSLHLNSLSLGHTTRPDFPILHQE+NGSKLVYLDNAATSQKPISVLNALQNYY+A
Sbjct: 90  TAKETDSLHLNSLSLGHTTRPDFPILHQEVNGSKLVYLDNAATSQKPISVLNALQNYYQA 149

Query: 121 YNSNVHRGIHSLSAKATDEYERARKKVASFINAGDAKEIIFTRNATEAINLVAYSWGLAN 180
           YNSNVHRGIH LSAKATDEYERARKKVASFINAGDAKEI+FTRNATEAINLVAYSWGLAN
Sbjct: 150 YNSNVHRGIHFLSAKATDEYERARKKVASFINAGDAKEIVFTRNATEAINLVAYSWGLAN 209

Query: 181 LKSEDEIILTVAEHHSAIVPWQLVAERTGAVLKFVSLGEHDVPNLKDFKEMFSTKTKLVV 240
           LKS DEIILTVAEHHSAIVPWQLVAERTGAVLKFV+LGEHDVPNLKD KEMFSTKTKLVV
Sbjct: 210 LKSGDEIILTVAEHHSAIVPWQLVAERTGAVLKFVNLGEHDVPNLKDLKEMFSTKTKLVV 269

Query: 241 THHVSNVLASVLPIEEIVGLAHRFGAKVLVDACQSVPHMVVNVQALDADFLVASSHKMCG 300
           THHVSNVLASVLPI EIVGLAHRFGAKVLVDACQSVPHMVV+VQALDADFLVASSHKMCG
Sbjct: 270 THHVSNVLASVLPIGEIVGLAHRFGAKVLVDACQSVPHMVVDVQALDADFLVASSHKMCG 329

Query: 301 PTGIGFLYGKIDLLSAMPPFLGGGEMISDVFLDHSTFAQPPSRFEAGTPAIGEAIGLGAA 360
           PTGIGFLYGKIDLLSAMPPFLGGGEMISDVFLDHSTFAQPPSRFEAGTPAIGEAIGLGAA
Sbjct: 330 PTGIGFLYGKIDLLSAMPPFLGGGEMISDVFLDHSTFAQPPSRFEAGTPAIGEAIGLGAA 389

Query: 361 IDYLSGIGMTKIHSYEEELANYLYENLRAVPNIRIYGPAPTAHVQRAALCSFNIGDIHPT 420
           IDYLSGIGM KIHSYEEELANYLYENLRAVPNIRIYGPAPTAHVQRAALCSFNIGDIHPT
Sbjct: 390 IDYLSGIGMQKIHSYEEELANYLYENLRAVPNIRIYGPAPTAHVQRAALCSFNIGDIHPT 449

Query: 421 DIATFLDQQHGVAIRSGHHCAQPLHRALGVNSSARVSLYFYNTKEDVDYFIQALNDTVSF 480
           DIATFLDQQHGVAIRSGHHCAQPLHRALGV+SSARVSLYFYNTKEDVDYFIQALNDTVSF
Sbjct: 450 DIATFLDQQHGVAIRSGHHCAQPLHRALGVSSSARVSLYFYNTKEDVDYFIQALNDTVSF 509

Query: 481 FDSFK 486
           FDSFK
Sbjct: 510 FDSFK 514

BLAST of MELO3C014533T3 vs. NCBI nr
Match: gi|802621477|ref|XP_012076008.1| (PREDICTED: cysteine desulfurase 1, chloroplastic isoform X1 [Jatropha curcas])

HSP 1 Score: 719.2 bits (1855), Expect = 5.0e-204
Identity = 366/490 (74.69%), Postives = 413/490 (84.29%), Query Frame = 1

Query: 1   MMEGMVLKLPSFHSQFQFQLMNPNRCISCFSSTLRASSSPL-LRFGNPRFSSPLS----V 60
           MM+G V KLPSF      + + PN      SS    + SP+ +R G  R SS  S    +
Sbjct: 3   MMDGAVFKLPSF------KFLRPN------SSPPATTKSPVVIRCGLTRLSSSYSSSSAI 62

Query: 61  SASASTAKETDSLHLNSLSLGHTTRPDFPILHQEINGSKLVYLDNAATSQKPISVLNALQ 120
            A A+T +E D   L SLSLGH TRPDFPILHQE+NGSKLVYLDNAATSQKP SVL ALQ
Sbjct: 63  QAIATTGREGDQA-LGSLSLGHITRPDFPILHQEVNGSKLVYLDNAATSQKPNSVLRALQ 122

Query: 121 NYYEAYNSNVHRGIHSLSAKATDEYERARKKVASFINAGDAKEIIFTRNATEAINLVAYS 180
           NYYE YNSNVHRGIH LSAKATDEYE ARKKVA+FINA D++EI+FTRNATEAINLVAYS
Sbjct: 123 NYYETYNSNVHRGIHYLSAKATDEYELARKKVAAFINASDSREIVFTRNATEAINLVAYS 182

Query: 181 WGLANLKSEDEIILTVAEHHSAIVPWQLVAERTGAVLKFVSLGEHDVPNLKDFKEMFSTK 240
           WGLANLK  DE+++T+AEHHSAIVPWQLVA+RTGA+LKFV+L E++VP+++  KE+ S  
Sbjct: 183 WGLANLKPGDEVVVTIAEHHSAIVPWQLVAQRTGALLKFVNLNENEVPDVQQLKEVISQN 242

Query: 241 TKLVVTHHVSNVLASVLPIEEIVGLAHRFGAKVLVDACQSVPHMVVNVQALDADFLVASS 300
           T+LVV HH+SNVLAS LPIE+I+  AH  GAKVL+DACQSVPHMVV+VQ L+ADFLVASS
Sbjct: 243 TRLVVVHHISNVLASFLPIEDIIEWAHDAGAKVLIDACQSVPHMVVDVQRLNADFLVASS 302

Query: 301 HKMCGPTGIGFLYGKIDLLSAMPPFLGGGEMISDVFLDHSTFAQPPSRFEAGTPAIGEAI 360
           HKMCGPTGIGFLYGK D+LS+MPPFLGGGEMISDVFLDHST+A+PPSRFEAGTPAIGEAI
Sbjct: 303 HKMCGPTGIGFLYGKSDILSSMPPFLGGGEMISDVFLDHSTYAEPPSRFEAGTPAIGEAI 362

Query: 361 GLGAAIDYLSGIGMTKIHSYEEELANYLYENLRAVPNIRIYGPAPTAHVQRAALCSFNIG 420
           GLGAAIDYL GIGM KIH YE ELANYLYE+L +VPN+RIYGP P+  V RAALCSFN+ 
Sbjct: 363 GLGAAIDYLLGIGMQKIHDYEMELANYLYESLSSVPNVRIYGPKPSETVHRAALCSFNVE 422

Query: 421 DIHPTDIATFLDQQHGVAIRSGHHCAQPLHRALGVNSSARVSLYFYNTKEDVDYFIQALN 480
            IHPTDIATFLDQQHGVAIRSGHHCAQPLHR LGVN+SAR SL+FYNTKEDVD FIQALN
Sbjct: 423 GIHPTDIATFLDQQHGVAIRSGHHCAQPLHRHLGVNASARASLHFYNTKEDVDDFIQALN 479

Query: 481 DTVSFFDSFK 486
           DT+SFF+SFK
Sbjct: 483 DTISFFNSFK 479

BLAST of MELO3C014533T3 vs. NCBI nr
Match: gi|643725520|gb|KDP34559.1| (hypothetical protein JCGZ_11109 [Jatropha curcas])

HSP 1 Score: 717.2 bits (1850), Expect = 1.9e-203
Identity = 365/489 (74.64%), Postives = 412/489 (84.25%), Query Frame = 1

Query: 2   MEGMVLKLPSFHSQFQFQLMNPNRCISCFSSTLRASSSPL-LRFGNPRFSSPLS----VS 61
           M+G V KLPSF      + + PN      SS    + SP+ +R G  R SS  S    + 
Sbjct: 1   MDGAVFKLPSF------KFLRPN------SSPPATTKSPVVIRCGLTRLSSSYSSSSAIQ 60

Query: 62  ASASTAKETDSLHLNSLSLGHTTRPDFPILHQEINGSKLVYLDNAATSQKPISVLNALQN 121
           A A+T +E D   L SLSLGH TRPDFPILHQE+NGSKLVYLDNAATSQKP SVL ALQN
Sbjct: 61  AIATTGREGDQA-LGSLSLGHITRPDFPILHQEVNGSKLVYLDNAATSQKPNSVLRALQN 120

Query: 122 YYEAYNSNVHRGIHSLSAKATDEYERARKKVASFINAGDAKEIIFTRNATEAINLVAYSW 181
           YYE YNSNVHRGIH LSAKATDEYE ARKKVA+FINA D++EI+FTRNATEAINLVAYSW
Sbjct: 121 YYETYNSNVHRGIHYLSAKATDEYELARKKVAAFINASDSREIVFTRNATEAINLVAYSW 180

Query: 182 GLANLKSEDEIILTVAEHHSAIVPWQLVAERTGAVLKFVSLGEHDVPNLKDFKEMFSTKT 241
           GLANLK  DE+++T+AEHHSAIVPWQLVA+RTGA+LKFV+L E++VP+++  KE+ S  T
Sbjct: 181 GLANLKPGDEVVVTIAEHHSAIVPWQLVAQRTGALLKFVNLNENEVPDVQQLKEVISQNT 240

Query: 242 KLVVTHHVSNVLASVLPIEEIVGLAHRFGAKVLVDACQSVPHMVVNVQALDADFLVASSH 301
           +LVV HH+SNVLAS LPIE+I+  AH  GAKVL+DACQSVPHMVV+VQ L+ADFLVASSH
Sbjct: 241 RLVVVHHISNVLASFLPIEDIIEWAHDAGAKVLIDACQSVPHMVVDVQRLNADFLVASSH 300

Query: 302 KMCGPTGIGFLYGKIDLLSAMPPFLGGGEMISDVFLDHSTFAQPPSRFEAGTPAIGEAIG 361
           KMCGPTGIGFLYGK D+LS+MPPFLGGGEMISDVFLDHST+A+PPSRFEAGTPAIGEAIG
Sbjct: 301 KMCGPTGIGFLYGKSDILSSMPPFLGGGEMISDVFLDHSTYAEPPSRFEAGTPAIGEAIG 360

Query: 362 LGAAIDYLSGIGMTKIHSYEEELANYLYENLRAVPNIRIYGPAPTAHVQRAALCSFNIGD 421
           LGAAIDYL GIGM KIH YE ELANYLYE+L +VPN+RIYGP P+  V RAALCSFN+  
Sbjct: 361 LGAAIDYLLGIGMQKIHDYEMELANYLYESLSSVPNVRIYGPKPSETVHRAALCSFNVEG 420

Query: 422 IHPTDIATFLDQQHGVAIRSGHHCAQPLHRALGVNSSARVSLYFYNTKEDVDYFIQALND 481
           IHPTDIATFLDQQHGVAIRSGHHCAQPLHR LGVN+SAR SL+FYNTKEDVD FIQALND
Sbjct: 421 IHPTDIATFLDQQHGVAIRSGHHCAQPLHRHLGVNASARASLHFYNTKEDVDDFIQALND 476

Query: 482 TVSFFDSFK 486
           T+SFF+SFK
Sbjct: 481 TISFFNSFK 476

BLAST of MELO3C014533T3 vs. NCBI nr
Match: gi|951044988|ref|XP_014518801.1| (PREDICTED: cysteine desulfurase 1, chloroplastic [Vigna radiata var. radiata])

HSP 1 Score: 715.7 bits (1846), Expect = 5.5e-203
Identity = 365/485 (75.26%), Postives = 414/485 (85.36%), Query Frame = 1

Query: 2   MEGMVLKLPSFHSQFQFQLMNPNRCISCFSSTLRASSSPLLRFGNPRFSSPLSVSASAST 61
           ME ++ KLPSF      +  +P+ C S    T+R+SS  ++RFG  R SS   +S SAST
Sbjct: 1   MEVLLPKLPSF------KFPSPSHCCSI---TIRSSS--IVRFGLRRVSS---LSVSAST 60

Query: 62  AKETDSLH-LNSLSLGHTTRPDFPILHQEINGSKLVYLDNAATSQKPISVLNALQNYYEA 121
             E  +   ++S SLGH+TRP FPILHQE+NGSKLVYLDNAATSQKP +VL ALQNYYE 
Sbjct: 61  VNEAVAQPAIDSPSLGHSTRPHFPILHQEVNGSKLVYLDNAATSQKPTNVLKALQNYYEG 120

Query: 122 YNSNVHRGIHSLSAKATDEYERARKKVASFINAGDAKEIIFTRNATEAINLVAYSWGLAN 181
           YNSNVHRGIH LSAKATDEYE AR+KVASFINA D++EI+FT+NA+EAINLVAYSWGL+N
Sbjct: 121 YNSNVHRGIHFLSAKATDEYESARRKVASFINASDSREIVFTKNASEAINLVAYSWGLSN 180

Query: 182 LKSEDEIILTVAEHHSAIVPWQLVAERTGAVLKFVSLGEHDVPNLKDFKEMFSTKTKLVV 241
           LK EDEIILTVAEHHSAIVPWQLVAE+ GAVLKFV L +  +P++   K++FS KTK+VV
Sbjct: 181 LKPEDEIILTVAEHHSAIVPWQLVAEKVGAVLKFVDLNQDGIPDIGKLKDLFSRKTKIVV 240

Query: 242 THHVSNVLASVLPIEEIVGLAHRFGAKVLVDACQSVPHMVVNVQALDADFLVASSHKMCG 301
            HHVSNVLASVLPI +I   AH  GAKVLVDACQSVPHMVV+VQ+L+ DFLVASSHKMCG
Sbjct: 241 VHHVSNVLASVLPIRDIAQWAHDVGAKVLVDACQSVPHMVVDVQSLNVDFLVASSHKMCG 300

Query: 302 PTGIGFLYGKIDLLSAMPPFLGGGEMISDVFLDHSTFAQPPSRFEAGTPAIGEAIGLGAA 361
           PTGIGFLYG+IDLLS+MPPFLGGGEMISDV+LDHST+A+PPSRFEAGTPAIGEAIGLGAA
Sbjct: 301 PTGIGFLYGRIDLLSSMPPFLGGGEMISDVYLDHSTYAEPPSRFEAGTPAIGEAIGLGAA 360

Query: 362 IDYLSGIGMTKIHSYEEELANYLYENLRAVPNIRIYGPAPTAHVQRAALCSFNIGDIHPT 421
           IDYLSGIGM  IH YE EL  YLYE L +VPNIRIYGPAP+ +VQRAALCSFN+ ++HPT
Sbjct: 361 IDYLSGIGMQTIHDYEVELGRYLYERLLSVPNIRIYGPAPSENVQRAALCSFNVENLHPT 420

Query: 422 DIATFLDQQHGVAIRSGHHCAQPLHRALGVNSSARVSLYFYNTKEDVDYFIQALNDTVSF 481
           D+ATFLDQQHGVAIRSGHHCAQPLHR LGV+SSAR SLYFYNTKEDVDYFI ALNDTVSF
Sbjct: 421 DLATFLDQQHGVAIRSGHHCAQPLHRFLGVSSSARASLYFYNTKEDVDYFIYALNDTVSF 471

Query: 482 FDSFK 486
           F+SFK
Sbjct: 481 FNSFK 471

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
CNIF1_ARATH4.2e-19571.69Cysteine desulfurase 1, chloroplastic OS=Arabidopsis thaliana GN=NFS2 PE=1 SV=1[more]
CSD_SYNY31.5e-14763.24Probable cysteine desulfurase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) GN... [more]
SUFS_BACSU2.3e-11651.99Cysteine desulfurase SufS OS=Bacillus subtilis (strain 168) GN=sufS PE=1 SV=1[more]
CSD_BACHD5.7e-11552.84Probable cysteine desulfurase OS=Bacillus halodurans (strain ATCC BAA-125 / DSM ... [more]
CSD_STAES6.3e-11451.75Probable cysteine desulfurase OS=Staphylococcus epidermidis (strain ATCC 12228) ... [more]
Match NameE-valueIdentityDescription
A0A067KRV2_JATCU1.3e-20374.64Uncharacterized protein OS=Jatropha curcas GN=JCGZ_11109 PE=4 SV=1[more]
A0A061GUT7_THECC6.6e-20376.08Chloroplastic NIFS-like cysteine desulfurase isoform 1 OS=Theobroma cacao GN=TCM... [more]
A0A0S3S493_PHAAN3.3e-20275.26Uncharacterized protein OS=Vigna angularis var. angularis GN=Vigan.05G104600 PE=... [more]
A0A0L9VET2_PHAAN3.3e-20275.26Uncharacterized protein OS=Phaseolus angularis GN=LR48_Vigan09g185300 PE=3 SV=1[more]
V7BND5_PHAVU7.2e-20274.79Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_006G131100g PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G08490.12.4e-19671.69 chloroplastic NIFS-like cysteine desulfurase[more]
Match NameE-valueIdentityDescription
gi|659097418|ref|XP_008449615.1|1.4e-278100.00PREDICTED: cysteine desulfurase 2, chloroplastic-like [Cucumis melo][more]
gi|778716806|ref|XP_004140178.2|2.4e-26796.08PREDICTED: cysteine desulfurase 1, chloroplastic [Cucumis sativus][more]
gi|802621477|ref|XP_012076008.1|5.0e-20474.69PREDICTED: cysteine desulfurase 1, chloroplastic isoform X1 [Jatropha curcas][more]
gi|643725520|gb|KDP34559.1|1.9e-20374.64hypothetical protein JCGZ_11109 [Jatropha curcas][more]
gi|951044988|ref|XP_014518801.1|5.5e-20375.26PREDICTED: cysteine desulfurase 1, chloroplastic [Vigna radiata var. radiata][more]
The following terms have been associated with this mRNA:
Vocabulary: INTERPRO
TermDefinition
IPR000192Aminotrans_V_dom
IPR010970Cys_dSase_SufS
IPR015421PyrdxlP-dep_Trfase_major
IPR015422PyrdxlP-dep_Trfase_dom1
IPR015424PyrdxlP-dep_Trfase
Vocabulary: Biological Process
TermDefinition
GO:0006534cysteine metabolic process
Vocabulary: Molecular Function
TermDefinition
GO:0030170pyridoxal phosphate binding
GO:0031071cysteine desulfurase activity
GO:0003824catalytic activity
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006534 cysteine metabolic process
biological_process GO:0009073 aromatic amino acid family biosynthetic process
biological_process GO:0018283 iron incorporation into metallo-sulfur cluster
biological_process GO:0055072 iron ion homeostasis
biological_process GO:0016226 iron-sulfur cluster assembly
biological_process GO:0010035 response to inorganic substance
biological_process GO:0010039 response to iron ion
biological_process GO:0000302 response to reactive oxygen species
biological_process GO:0010269 response to selenium ion
biological_process GO:0001887 selenium compound metabolic process
cellular_component GO:0009507 chloroplast
molecular_function GO:0003824 catalytic activity
molecular_function GO:0031071 cysteine desulfurase activity
molecular_function GO:0030170 pyridoxal phosphate binding
molecular_function GO:0016829 lyase activity
molecular_function GO:0009000 selenocysteine lyase activity

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
MELO3C014533MELO3C014533gene


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
MELO3C014533T3MELO3C014533T3-proteinpolypeptide


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
MELO3C014533T3.cds10MELO3C014533T3.cds10CDS
MELO3C014533T3.cds9MELO3C014533T3.cds9CDS
MELO3C014533T3.cds8MELO3C014533T3.cds8CDS
MELO3C014533T3.cds7MELO3C014533T3.cds7CDS
MELO3C014533T3.cds6MELO3C014533T3.cds6CDS
MELO3C014533T3.cds5MELO3C014533T3.cds5CDS
MELO3C014533T3.cds4MELO3C014533T3.cds4CDS
MELO3C014533T3.cds3MELO3C014533T3.cds3CDS
MELO3C014533T3.cds2MELO3C014533T3.cds2CDS
MELO3C014533T3.cds1MELO3C014533T3.cds1CDS


Analysis Name: InterPro Annotations of melon
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000192Aminotransferase class V domainPFAMPF00266Aminotran_5coord: 96..470
score: 4.5E
IPR010970Cysteine desulfurase, SufSTIGRFAMsTIGR01979TIGR01979coord: 79..481
score: 7.6E
IPR015421Pyridoxal phosphate-dependent transferase, major region, subdomain 1GENE3DG3DSA:3.40.640.10coord: 81..329
score: 1.1
IPR015422Pyridoxal phosphate-dependent transferase, major region, subdomain 2GENE3DG3DSA:3.90.1150.10coord: 330..485
score: 1.4
IPR015424Pyridoxal phosphate-dependent transferaseunknownSSF53383PLP-dependent transferasescoord: 79..477
score: 5.91E
NoneNo IPR availablePANTHERPTHR11601CYSTEINE DESULFURYLASEcoord: 1..485
score: 1.3E
NoneNo IPR availablePANTHERPTHR11601:SF17CYSTEINE DESULFURASE 2, CHLOROPLASTICcoord: 1..485
score: 1.3E