MELO3C014533 (gene) Melon (DHL92) v3.5.1

NameMELO3C014533
Typegene
OrganismCucumis melo (Melon (DHL92) v3.5.1)
DescriptionCysteine desulfurase family protein
Locationchr5 : 1564259 .. 1568189 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
GAAGCTTTTTCGATTCTCAAAAACCATAGAGGATGATGGAAGGTATGGTTCTGAAGCTCCCATCCTTCCATTCCCAATTTCAATTCCAGCTCATGAACCCTAATCGTTGTATTTCTTGTTTCTCTTCCACCCTTCGTGCTTCCTCTTCTCCTCTTCTCCGCTTCGGTAATCCCAGATTTTCCTCTCCCTTGTCAGTTTCTGCTTCTGCTTCCACCGCCAAGGAAACCGACAGCTTGCATCTCAACTCTCTTTCGCTTGGCCACACAACTCGCCCCGATTTCCCCATCCTCCATCAGGTTCTTGTTTCACTTTTTATTCTCTTGTTTTTTTTTTTCCTTTGCTAATTTGGCCGATTTTTATGTTAACCCATTTGTCGTCTGTTTCGCTGTTATTGGCACTTCATGTGGTGGTGTTAATGGCTGTATTGCTATCTGCAGGAAATAAATGGCTCCAAGCTTGTCTACTTAGACAACGCTGCCACTTCTCAGAAACCAATTTCTGTGTTGAATGCTTTGCAGAATTACTATGAGGCTTACAACTCAAATGTGCATCGTGGCATACACTCTTTGAGGTAGGTTATTTAGACTTTGGTATGAAACGTGAATGAAAACGACTTCTATCTGATTTGAAACCGTTGGCAAATGTAGTGCGAAAGCAACTGATGAGTATGAGCGGGCAAGGAAGAAAGTTGCATCCTTTATCAATGCTGGAGATGCTAAAGAGATCATTTTTACCAGGAATGCAACTGAAGCTATCAATCTAGTGGCTTATTCTTGGGGCCTAGCGAATCTGAAATCGGAAGATGAGGTGCGTCGCCACTGCATCGATCTTAGTAGTTTTGCACAAGTGATTTTCTTCTATATATGTACTATTTAGTGCACGTATAAGCACTGACTCTGTGAGACATGGTATTCTCCATATGGGGTTAAATTGCAAATTTAATTCCAATGGTCGGAAGAAAGTTAGAATTTAGTCCTATGATTGGGACTATTTATGAAATGTTTATGAAATCATAGGGTAGTTTATCAAACCGTCAGGACTATTAGCTGTCTCTCTCATTTCTAATCTGTAAATTTCAATGGGTTTACATTTTATACTTGTTTGTATTCTTGTTGATTGTCTAAGGTGTAGGCTTCAGGGTTCTCTTTGTGAAGATTCTGATGTCAGATTTAATTAAGTTAATAGAAGTGTTTTCCATTCCTACATCATGAAGTTGCATCCAGTATTTTTTTAAATGTGTAGCCTCATACTGCATTAACTTTAAGTTTTAACTGTAACAATCTTTTCTTTCAGTGCAGATCATACTTACGGTTGCTGAACATCATAGTGCCATTGTTCCTTGGCAACTTGTAGCTGAAAGGACCGGTGCTGTTCTGAAGTTTGTAAGTTTAGGTGAACATGATGTCCCAAACTTAAAGGACTTCAAGGAAATGTTTTCAACAAAAACGAAGCTTGTGGTTACCCATCATGTCTCAAATGTACTAGGTACTTCCTTCTATTTGATATTTAGCATTTTTATAATCTATTTCCTTTTTGTACCATTCAATTTGTAAACATTACTCAAATAAAACAAGTAGTAGGTGAATATTTCAAACTGAAGTGTTATCATATTACTTCAATTATCACACTTCTAGTGGAAAGGTGTGCAGCTGGATACAAATAATAAAGTCCAATGAATGAAGAAGTGAAAGAACTGTTGGACATTTTTCCTTCTCTTGATCTGTGTCCGGTTTCTCTTGATATCTTTCTAATAATAAGTCTATGCACATAGTGGAATTCGGTATGATTTGATTGTTTTTCTGCAGCTTCTGTTCTTCCAATTGAAGAAATAGTTGGCTTGGCACATCGTTTTGGGGCAAAAGTGCTCGTAGATGCATGTCAGAGTGTTCCACATATGGTTGTCAACGTCCAGGCGCTTGATGCTGACTTTCTTGTTGCTTCTTCTCACAAGGTTAGGCTGATTTCTTATTAATTCAATGGGTAGCCTTACATTTGCGCATGTGAAGTTGAGGAATAAACATGCTTCAGATGTGTGGGCCTACAGGCATCGGATTCTTGTATGGAAAGATTGACCTGCTGTCTGCAATGCCTCCATTCTTAGGTATATCCTTCGAGAAGATAAGTTATGAAAATAAAATTGCATCAGCTCGATTTTAGGAAGTAAGTATCTAATTTTCATATTTGGTCCTACATGGCTCTAGGTGGTGGAGAGATGATCTCAGATGTATTTCTTGACCATTCCACATTTGCACAACCACCTTCCAGGTAGTGGACTGCATGGGTGCAATTTTGTCCTTTTCTTTTTACTTTGAAATGAAAATTTTCTCAATGTATTTATTTTATATGATGCGGAAAAGTTAGAACGTTCAATATATAGGGCTCAAGAATTGCAATAGAGCTAAATAACTGCTGTTTATTTAGTATTATTTGTTTGCTCATTTGTTCTCTTAGCAATTGACACTTGATAAATCATCTTTTTGGGGTCATAATGAGATCTAAATAAATGACTTCTTGAATGGTGCTAAAATCTTGTGATGGCTATTTTATTTTAACTATTCATTAATTGATGCCCTTTTACATATCATTTATGTCTTCTATGTAATCTCTTCCTGTCCAGATGCCTTTTGATCTTTGCATTAAAAAATCTTTTTGGTGGACTATTTTTTGTCCTTAATTCTACTAATTACTCATTTCCCCCTTCATTAGATTTGAAGCTGGGACGCCGGCGATTGGAGAGGCAATTGGTTTGGGAGCTGCTATTGATTATTTATCTGGGATCGGAATGACAAAAATTCATAGTTATGAAGTGAGTTGACTGAAATTTGGTATATAATAACCCTTTGTTTGAGAATTTTTGGGCGGTGAAAAAATGTGTGAAGCAACTAAATCAGGGTATCAACTAAATACCTTTATAGTAGATTAGTAATGGAATTTTCTTTAATCAAGGAATTTAGGTATTATTTTTCAATCAACTAGGTTACTTGGAACCCTGAGAAATATAGAATGAGATGATAAATATGTTTACGAGTTTTGAGACTTTAGTCTAACGACGAGGAGGTGCTGTCTGCTAACAGGAAGAATTGGCAAATTATCTATATGAAAACCTTCGTGCGGTCCCTAACATTCGGATATATGGGCCAGCTCCCACAGCACATGTTCAACGGGCTGCGCTGTGTTCTTTCAATATTGGAGACATTCATCCAACAGACATTGCAACATTTCTCGATCAACAGGTATGTTTTGGACGTTTGTCCCTATGAAATACTTTTGATGTAAGATTCCAATCCTTGAACAAAATCTTTAAGAGCCAAGGAACCAAAATCAAATAATGATGTAAGTTTCGAACTAAAGTAGAATTTAATTTAAATCCTTTCATTAACCTTTTCAGTCTCAAGGACTTGGGCTAATATCATTAGTTCATTACTGACTTATAGCTAGAGTGAATCATCTACTTTCTATAGATCGGATTCTTAAAATATTTTAATGCAGCATGGTGTAGCCATCAGATCAGGACATCACTGTGCGCAGCCCCTCCATCGGGCTCTTGGTGTGAACTCAAGTGCTCGAGTTAGCCTTTACTTCTACAACACAAAAGAGGATGTGGACTACTTTATCCAGGCTCTAAACGACACCGTAAGTTTCTTCGACTCTTTCAAGTAGAGAGATCCAGTAATACCGAACTCCTTTGTTTGACGGTCCACAGCTCGTACTTAACCTTCTGGACCACAAGGATTATGATATGTTTCTCGCTTTGAAGAAGATTGTCTGACTTTATCTTTCAGTCACATTTACTACCAGATCCTTGTGCATATAGTCACATTCTCATAGTTTTTTAGACTTAAGTGTATTTATAAATTATTTGATTAAGCAATGGTTTCATGGACTACATTTTATCTCCATAATATCAATGTACATCGACAGTTACCTGCCT

mRNA sequence

GAAGCTTTTTCGATTCTCAAAAACCATAGAGGATGATGGAAGGTATGGTTCTGAAGCTCCCATCCTTCCATTCCCAATTTCAATTCCAGCTCATGAACCCTAATCGTTGTATTTCTTGTTTCTCTTCCACCCTTCGTGCTTCCTCTTCTCCTCTTCTCCGCTTCGGTAATCCCAGATTTTCCTCTCCCTTGTCAGTTTCTGCTTCTGCTTCCACCGCCAAGGAAACCGACAGCTTGCATCTCAACTCTCTTTCGCTTGGCCACACAACTCGCCCCGATTTCCCCATCCTCCATCAGGAAATAAATGGCTCCAAGCTTGTCTACTTAGACAACGCTGCCACTTCTCAGAAACCAATTTCTGTGTTGAATGCTTTGCAGAATTACTATGAGGCTTACAACTCAAATGTGCATCGTGGCATACACTCTTTGAGTGCGAAAGCAACTGATGAGTATGAGCGGGCAAGGAAGAAAGTTGCATCCTTTATCAATGCTGGAGATGCTAAAGAGATCATTTTTACCAGGAATGCAACTGAAGCTATCAATCTAGTGGCTTATTCTTGGGGCCTAGCGAATCTGAAATCGGAAGATGAGATCATACTTACGGTTGCTGAACATCATAGTGCCATTGTTCCTTGGCAACTTGTAGCTGAAAGGACCGGTGCTGTTCTGAAGTTTGTAAGTTTAGGTGAACATGATGTCCCAAACTTAAAGGACTTCAAGGAAATGTTTTCAACAAAAACGAAGCTTGTGGTTACCCATCATGTCTCAAATGTACTAGCTTCTGTTCTTCCAATTGAAGAAATAGTTGGCTTGGCACATCGTTTTGGGGCAAAAGTGCTCGTAGATGCATGTCAGAGTGTTCCACATATGGTTGTCAACGTCCAGGCGCTTGATGCTGACTTTCTTGTTGCTTCTTCTCACAAGATGTGTGGGCCTACAGGCATCGGATTCTTGTATGGAAAGATTGACCTGCTGTCTGCAATGCCTCCATTCTTAGGTGGTGGAGAGATGATCTCAGATGTATTTCTTGACCATTCCACATTTGCACAACCACCTTCCAGATTTGAAGCTGGGACGCCGGCGATTGGAGAGGCAATTGGTTTGGGAGCTGCTATTGATTATTTATCTGGGATCGGAATGACAAAAATTCATAGTTATGAAGAAGAATTGGCAAATTATCTATATGAAAACCTTCGTGCGGTCCCTAACATTCGGATATATGGGCCAGCTCCCACAGCACATGTTCAACGGGCTGCGCTGTGTTCTTTCAATATTGGAGACATTCATCCAACAGACATTGCAACATTTCTCGATCAACAGCATGGTGTAGCCATCAGATCAGGACATCACTGTGCGCAGCCCCTCCATCGGGCTCTTGGTGTGAACTCAAGTGCTCGAGTTAGCCTTTACTTCTACAACACAAAAGAGGATGTGGACTACTTTATCCAGGCTCTAAACGACACCGTAAGTTTCTTCGACTCTTTCAAGTAGAGAGATCCAGTAATACCGAACTCCTTTGTTTGACGGTCCACAGCTCGTACTTAACCTTCTGGACCACAAGGATTATGATATGTTTCTCGCTTTGAAGAAGATTGTCTGACTTTATCTTTCAGTCACATTTACTACCAGATCCTTGTGCATATAGTCACATTCTCATAGTTTTTTAGACTTAAGTGTATTTATAAATTATTTGATTAAGCAATGGTTTCATGGACTACATTTTATCTCCATAATATCAATGTACATCGACAGTTACCTGCCT

Coding sequence (CDS)

ATGATGGAAGGTATGGTTCTGAAGCTCCCATCCTTCCATTCCCAATTTCAATTCCAGCTCATGAACCCTAATCGTTGTATTTCTTGTTTCTCTTCCACCCTTCGTGCTTCCTCTTCTCCTCTTCTCCGCTTCGGTAATCCCAGATTTTCCTCTCCCTTGTCAGTTTCTGCTTCTGCTTCCACCGCCAAGGAAACCGACAGCTTGCATCTCAACTCTCTTTCGCTTGGCCACACAACTCGCCCCGATTTCCCCATCCTCCATCAGGAAATAAATGGCTCCAAGCTTGTCTACTTAGACAACGCTGCCACTTCTCAGAAACCAATTTCTGTGTTGAATGCTTTGCAGAATTACTATGAGGCTTACAACTCAAATGTGCATCGTGGCATACACTCTTTGAGTGCGAAAGCAACTGATGAGTATGAGCGGGCAAGGAAGAAAGTTGCATCCTTTATCAATGCTGGAGATGCTAAAGAGATCATTTTTACCAGGAATGCAACTGAAGCTATCAATCTAGTGGCTTATTCTTGGGGCCTAGCGAATCTGAAATCGGAAGATGAGATCATACTTACGGTTGCTGAACATCATAGTGCCATTGTTCCTTGGCAACTTGTAGCTGAAAGGACCGGTGCTGTTCTGAAGTTTGTAAGTTTAGGTGAACATGATGTCCCAAACTTAAAGGACTTCAAGGAAATGTTTTCAACAAAAACGAAGCTTGTGGTTACCCATCATGTCTCAAATGTACTAGCTTCTGTTCTTCCAATTGAAGAAATAGTTGGCTTGGCACATCGTTTTGGGGCAAAAGTGCTCGTAGATGCATGTCAGAGTGTTCCACATATGGTTGTCAACGTCCAGGCGCTTGATGCTGACTTTCTTGTTGCTTCTTCTCACAAGATGTGTGGGCCTACAGGCATCGGATTCTTGTATGGAAAGATTGACCTGCTGTCTGCAATGCCTCCATTCTTAGGTGGTGGAGAGATGATCTCAGATGTATTTCTTGACCATTCCACATTTGCACAACCACCTTCCAGATTTGAAGCTGGGACGCCGGCGATTGGAGAGGCAATTGGTTTGGGAGCTGCTATTGATTATTTATCTGGGATCGGAATGACAAAAATTCATAGTTATGAAGAAGAATTGGCAAATTATCTATATGAAAACCTTCGTGCGGTCCCTAACATTCGGATATATGGGCCAGCTCCCACAGCACATGTTCAACGGGCTGCGCTGTGTTCTTTCAATATTGGAGACATTCATCCAACAGACATTGCAACATTTCTCGATCAACAGCATGGTGTAGCCATCAGATCAGGACATCACTGTGCGCAGCCCCTCCATCGGGCTCTTGGTGTGAACTCAAGTGCTCGAGTTAGCCTTTACTTCTACAACACAAAAGAGGATGTGGACTACTTTATCCAGGCTCTAAACGACACCGTAAGTTTCTTCGACTCTTTCAAGTAG

Protein sequence

MMEGMVLKLPSFHSQFQFQLMNPNRCISCFSSTLRASSSPLLRFGNPRFSSPLSVSASASTAKETDSLHLNSLSLGHTTRPDFPILHQEINGSKLVYLDNAATSQKPISVLNALQNYYEAYNSNVHRGIHSLSAKATDEYERARKKVASFINAGDAKEIIFTRNATEAINLVAYSWGLANLKSEDEIILTVAEHHSAIVPWQLVAERTGAVLKFVSLGEHDVPNLKDFKEMFSTKTKLVVTHHVSNVLASVLPIEEIVGLAHRFGAKVLVDACQSVPHMVVNVQALDADFLVASSHKMCGPTGIGFLYGKIDLLSAMPPFLGGGEMISDVFLDHSTFAQPPSRFEAGTPAIGEAIGLGAAIDYLSGIGMTKIHSYEEELANYLYENLRAVPNIRIYGPAPTAHVQRAALCSFNIGDIHPTDIATFLDQQHGVAIRSGHHCAQPLHRALGVNSSARVSLYFYNTKEDVDYFIQALNDTVSFFDSFK*
BLAST of MELO3C014533 vs. Swiss-Prot
Match: CNIF1_ARATH (Cysteine desulfurase 1, chloroplastic OS=Arabidopsis thaliana GN=NFS2 PE=1 SV=1)

HSP 1 Score: 367.9 bits (943), Expect = 1.1e-100
Identity = 197/296 (66.55%), Postives = 224/296 (75.68%), Query Frame = 1

Query: 2   MEGMVLKLPSFHSQFQFQLMNPNRCISCFSSTLRASSSPLLRFGNPRFSSPLSVSASAST 61
           MEG+ +KLPSF          PN           A S     F   R SS LSV ++A+ 
Sbjct: 1   MEGVAMKLPSF----------PN-----------AISIGHRSFSRVRCSSSLSVCSAAAA 60

Query: 62  AKETDSLHLNSLSLGHTTRPDFPILHQEINGSKLVYLDNAATSQKPISVLNALQNYYEAY 121
           +  T S    S+SLGH  R DF ILHQE+NGSKLVYLD+AATSQKP +VL+ALQNYYE Y
Sbjct: 61  SSATISTDSESVSLGHRVRKDFRILHQEVNGSKLVYLDSAATSQKPAAVLDALQNYYEFY 120

Query: 122 NSNVHRGIHSLSAKATDEYERARKKVASFINAGDAKEIIFTRNATEAINLVAYSWGLANL 181
           NSNVHRGIH LSAKATDE+E ARKKVA FINA D++EI+FTRNATEAINLVAYSWGL+NL
Sbjct: 121 NSNVHRGIHYLSAKATDEFELARKKVARFINASDSREIVFTRNATEAINLVAYSWGLSNL 180

Query: 182 KSEDEIILTVAEHHSAIVPWQLVAERTGAVLKFVSLGEHDVPNLKDFKEMFSTKTKLVVT 241
           K  DE+ILTVAEHHS IVPWQ+V+++TGAVLKFV+L E +VP++   +E+ S KTKLV  
Sbjct: 181 KPGDEVILTVAEHHSCIVPWQIVSQKTGAVLKFVTLNEDEVPDINKLRELISPKTKLVAV 240

Query: 242 HHVSNVLASVLPIEEIVGLAHRFGAKVLVDACQSVPHMVVNVQALDADFLVASSHK 298
           HHVSNVLAS LPIEEIV  AH  GAKVLVDACQSVPHMVV+VQ L+ADFLVASSHK
Sbjct: 241 HHVSNVLASSLPIEEIVVWAHDVGAKVLVDACQSVPHMVVDVQKLNADFLVASSHK 275

BLAST of MELO3C014533 vs. Swiss-Prot
Match: CSD_SYNY3 (Probable cysteine desulfurase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) GN=csd PE=1 SV=1)

HSP 1 Score: 287.3 bits (734), Expect = 2.0e-76
Identity = 146/224 (65.18%), Postives = 167/224 (74.55%), Query Frame = 1

Query: 74  SLGHTTRPDFPILHQEINGSKLVYLDNAATSQKPISVLNALQNYYEAYNSNVHRGIHSLS 133
           SL  T R DFPIL+QEING  LVYLDNAATSQKP +VL  L +YYE  N+NVHRG H LS
Sbjct: 8   SLAATVRQDFPILNQEINGHPLVYLDNAATSQKPRAVLEKLMHYYENDNANVHRGAHQLS 67

Query: 134 AKATDEYERARKKVASFINAGDAKEIIFTRNATEAINLVAYSWGLANLKSEDEIILTVAE 193
            +ATD YE  R KVA FINA   +EI++TRNATEAINLVAYSWG+ NLK+ DEII TV E
Sbjct: 68  VRATDAYEAVRNKVAKFINARSPREIVYTRNATEAINLVAYSWGMNNLKAGDEIITTVME 127

Query: 194 HHSAIVPWQLVAERTGAVLKFVSLGEHDVPNLKDFKEMFSTKTKLVVTHHVSNVLASVLP 253
           HHS +VPWQ+VA +TGAVLKFV L E +  +L+ FK + S KTKLV   H+SN L  V P
Sbjct: 128 HHSNLVPWQMVAAKTGAVLKFVQLDEQESFDLEHFKTLLSEKTKLVTVVHISNTLGCVNP 187

Query: 254 IEEIVGLAHRFGAKVLVDACQSVPHMVVNVQALDADFLVASSHK 298
            EEI  LAH+ GAKVLVDACQS PH  ++VQ +D D+LVAS HK
Sbjct: 188 AEEIAQLAHQAGAKVLVDACQSAPHYPLDVQLIDCDWLVASGHK 231

BLAST of MELO3C014533 vs. Swiss-Prot
Match: CSD_BACHD (Probable cysteine desulfurase OS=Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) GN=csd PE=3 SV=2)

HSP 1 Score: 244.6 bits (623), Expect = 1.5e-63
Identity = 121/221 (54.75%), Postives = 153/221 (69.23%), Query Frame = 1

Query: 77  HTTRPDFPILHQEINGSKLVYLDNAATSQKPISVLNALQNYYEAYNSNVHRGIHSLSAKA 136
           H  +  FP+L QE+NGS LVYLD+AATSQKPI+V+ AL +YY  YNSNVHRG+H+L   A
Sbjct: 4   HEIKKLFPVLDQEVNGSPLVYLDSAATSQKPIAVIEALDDYYRRYNSNVHRGVHTLGTLA 63

Query: 137 TDEYERARKKVASFINAGDAKEIIFTRNATEAINLVAYSWGLANLKSEDEIILTVAEHHS 196
           TD YE AR+K+  FI+A   +EIIFTR  T AINLVA S+G ANL   DEI++T  EHHS
Sbjct: 64  TDGYEGAREKIRRFIHASSTEEIIFTRGTTTAINLVAASYGRANLGEGDEIVITPMEHHS 123

Query: 197 AIVPWQLVAERTGAVLKFVSLGEHDVPNLKDFKEMFSTKTKLVVTHHVSNVLASVLPIEE 256
            I+PWQ VA+ TGA L ++ L +     ++D ++  S KTK+V   HVSNVL ++ P++E
Sbjct: 124 NIIPWQQVAKATGATLTYLPLQKDGTIKIEDVEKTISEKTKIVAIMHVSNVLGTINPVKE 183

Query: 257 IVGLAHRFGAKVLVDACQSVPHMVVNVQALDADFLVASSHK 298
           I  +AHR GA +LVD  QS PHM ++VQ L  DF   S HK
Sbjct: 184 IAEIAHRHGAIMLVDGAQSAPHMKIDVQELGCDFFAFSGHK 224

BLAST of MELO3C014533 vs. Swiss-Prot
Match: SUFS_BACSU (Cysteine desulfurase SufS OS=Bacillus subtilis (strain 168) GN=sufS PE=1 SV=1)

HSP 1 Score: 241.9 bits (616), Expect = 9.5e-63
Identity = 116/218 (53.21%), Postives = 150/218 (68.81%), Query Frame = 1

Query: 80  RPDFPILHQEINGSKLVYLDNAATSQKPISVLNALQNYYEAYNSNVHRGIHSLSAKATDE 139
           R  FPILHQ++NG  LVYLD+AATSQKP +V+  L  YY  YNSNVHRG+H+L  +ATD 
Sbjct: 7   REQFPILHQQVNGHDLVYLDSAATSQKPRAVIETLDKYYNQYNSNVHRGVHTLGTRATDG 66

Query: 140 YERARKKVASFINAGDAKEIIFTRNATEAINLVAYSWGLANLKSEDEIILTVAEHHSAIV 199
           YE AR+KV  FINA    EIIFT+  T ++N+VA S+  ANLK  DE+++T  EHH+ I+
Sbjct: 67  YEGAREKVRKFINAKSMAEIIFTKGTTTSLNMVALSYARANLKPGDEVVITYMEHHANII 126

Query: 200 PWQLVAERTGAVLKFVSLGEHDVPNLKDFKEMFSTKTKLVVTHHVSNVLASVLPIEEIVG 259
           PWQ   + TGA LK++ L E    +L+D +E  ++ TK+V   HVSNVL +V PI+E+  
Sbjct: 127 PWQQAVKATGATLKYIPLQEDGTISLEDVRETVTSNTKIVAVSHVSNVLGTVNPIKEMAK 186

Query: 260 LAHRFGAKVLVDACQSVPHMVVNVQALDADFLVASSHK 298
           +AH  GA ++VD  QS PHM ++VQ LD DF   SSHK
Sbjct: 187 IAHDNGAVIVVDGAQSTPHMKIDVQDLDCDFFALSSHK 224

BLAST of MELO3C014533 vs. Swiss-Prot
Match: CSD_MYCPA (Probable cysteine desulfurase OS=Mycobacterium paratuberculosis (strain ATCC BAA-968 / K-10) GN=MAP_2120c PE=3 SV=1)

HSP 1 Score: 228.0 bits (580), Expect = 1.4e-58
Identity = 107/218 (49.08%), Postives = 149/218 (68.35%), Query Frame = 1

Query: 80  RPDFPILHQEINGSKLVYLDNAATSQKPISVLNALQNYYEAYNSNVHRGIHSLSAKATDE 139
           R DFPIL + +NG  L++ DNAAT+QKP  V++ L ++Y   NSN+HR  H L+A+ATD 
Sbjct: 285 RSDFPILKETVNGKPLIWFDNAATTQKPQVVIDRLSHFYAHENSNIHRAAHELAARATDA 344

Query: 140 YERARKKVASFINAGDAKEIIFTRNATEAINLVAYSWGLANLKSEDEIILTVAEHHSAIV 199
           YE AR  VA FI A  +  I+F R  TEAINLVA++WG  +L+  DEI++T  EHH+ IV
Sbjct: 345 YEEARDTVAEFIGAPSSDNIVFVRGTTEAINLVAHAWGAKHLQPGDEIVITHLEHHANIV 404

Query: 200 PWQLVAERTGAVLKFVSLGEHDVPNLKDFKEMFSTKTKLVVTHHVSNVLASVLPIEEIVG 259
           PWQL++++TGA+LK   + +     L +F+ +   +TKLV   HVSN L +V+P+++IV 
Sbjct: 405 PWQLISQKTGAILKVAPIDDAGNLLLSEFEGLLGPRTKLVAASHVSNALGTVMPVDKIVE 464

Query: 260 LAHRFGAKVLVDACQSVPHMVVNVQALDADFLVASSHK 298
           L HR+GA+VL+D  QS+ H+ ++V  L ADF V S HK
Sbjct: 465 LGHRYGARVLIDGAQSIQHIPIDVAELGADFFVFSGHK 502

BLAST of MELO3C014533 vs. TrEMBL
Match: A0A0A0KEG9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G427980 PE=4 SV=1)

HSP 1 Score: 551.2 bits (1419), Expect = 8.2e-154
Identity = 280/297 (94.28%), Postives = 285/297 (95.96%), Query Frame = 1

Query: 1   MMEGMVLKLPSFHSQFQFQLMNPNRCISCFSSTLRASSSPLLRFGNPRFSSPLSVSASAS 60
           MMEGMVLK PSFHSQF FQ MNPNRCISCFSSTLRASSSPLLRF NPR  S  SVSASAS
Sbjct: 30  MMEGMVLKSPSFHSQFHFQFMNPNRCISCFSSTLRASSSPLLRFRNPRLPSHFSVSASAS 89

Query: 61  TAKETDSLHLNSLSLGHTTRPDFPILHQEINGSKLVYLDNAATSQKPISVLNALQNYYEA 120
           TAKETDSLHLNSLSLGHTTRPDFPILHQE+NGSKLVYLDNAATSQKPISVLNALQNYY+A
Sbjct: 90  TAKETDSLHLNSLSLGHTTRPDFPILHQEVNGSKLVYLDNAATSQKPISVLNALQNYYQA 149

Query: 121 YNSNVHRGIHSLSAKATDEYERARKKVASFINAGDAKEIIFTRNATEAINLVAYSWGLAN 180
           YNSNVHRGIH LSAKATDEYERARKKVASFINAGDAKEI+FTRNATEAINLVAYSWGLAN
Sbjct: 150 YNSNVHRGIHFLSAKATDEYERARKKVASFINAGDAKEIVFTRNATEAINLVAYSWGLAN 209

Query: 181 LKSEDEIILTVAEHHSAIVPWQLVAERTGAVLKFVSLGEHDVPNLKDFKEMFSTKTKLVV 240
           LKS DEIILTVAEHHSAIVPWQLVAERTGAVLKFV+LGEHDVPNLKD KEMFSTKTKLVV
Sbjct: 210 LKSGDEIILTVAEHHSAIVPWQLVAERTGAVLKFVNLGEHDVPNLKDLKEMFSTKTKLVV 269

Query: 241 THHVSNVLASVLPIEEIVGLAHRFGAKVLVDACQSVPHMVVNVQALDADFLVASSHK 298
           THHVSNVLASVLPI EIVGLAHRFGAKVLVDACQSVPHMVV+VQALDADFLVASSHK
Sbjct: 270 THHVSNVLASVLPIGEIVGLAHRFGAKVLVDACQSVPHMVVDVQALDADFLVASSHK 326

BLAST of MELO3C014533 vs. TrEMBL
Match: A0A061GU52_THECC (Chloroplastic NIFS-like cysteine desulfurase isoform 2 OS=Theobroma cacao GN=TCM_040966 PE=4 SV=1)

HSP 1 Score: 388.7 bits (997), Expect = 7.0e-105
Identity = 210/297 (70.71%), Postives = 234/297 (78.79%), Query Frame = 1

Query: 1   MMEGMVLKLPSFHSQFQFQLMNPNRCISCFSSTLRASSSPLLRFGNPRFSSPLSVSASAS 60
           MMEG+V+KLPSF       L+NP          L +SS PL R G  RFS      AS S
Sbjct: 4   MMEGVVVKLPSF------PLLNPT------IPPLSSSSLPL-RLGFRRFS------ASLS 63

Query: 61  TAKETDSLHLNSLSLGHTTRPDFPILHQEINGSKLVYLDNAATSQKPISVLNALQNYYEA 120
             KE       S+SLGH +RPDFPILHQE+NGSKLVYLDNAATSQKP +VL AL NYYEA
Sbjct: 64  AVKE------GSVSLGHLSRPDFPILHQEVNGSKLVYLDNAATSQKPTAVLKALHNYYEA 123

Query: 121 YNSNVHRGIHSLSAKATDEYERARKKVASFINAGDAKEIIFTRNATEAINLVAYSWGLAN 180
           YNSNVHRGIH LSAKATDEYE ARKKVA+FINA D+ EI+FTRNATEAINLVAYSWGL N
Sbjct: 124 YNSNVHRGIHYLSAKATDEYELARKKVAAFINASDSSEIVFTRNATEAINLVAYSWGLLN 183

Query: 181 LKSEDEIILTVAEHHSAIVPWQLVAERTGAVLKFVSLGEHDVPNLKDFKEMFSTKTKLVV 240
           LK  DEI+LT+AEHHSAIVPWQ+VA++TGAVLKFVSL E+++PN+   +EM ST+T+LVV
Sbjct: 184 LKPGDEIVLTIAEHHSAIVPWQIVAQKTGAVLKFVSLDENEIPNVVKLREMISTRTELVV 243

Query: 241 THHVSNVLASVLPIEEIVGLAHRFGAKVLVDACQSVPHMVVNVQALDADFLVASSHK 298
            HHVSNVL SVLPIEEIV   H  GAKVLVDACQSVPHMVV+VQ LDADFLVASSHK
Sbjct: 244 VHHVSNVLGSVLPIEEIVHWVHEVGAKVLVDACQSVPHMVVDVQGLDADFLVASSHK 275

BLAST of MELO3C014533 vs. TrEMBL
Match: A0A061GUT7_THECC (Chloroplastic NIFS-like cysteine desulfurase isoform 1 OS=Theobroma cacao GN=TCM_040966 PE=4 SV=1)

HSP 1 Score: 388.7 bits (997), Expect = 7.0e-105
Identity = 210/297 (70.71%), Postives = 234/297 (78.79%), Query Frame = 1

Query: 1   MMEGMVLKLPSFHSQFQFQLMNPNRCISCFSSTLRASSSPLLRFGNPRFSSPLSVSASAS 60
           MMEG+V+KLPSF       L+NP          L +SS PL R G  RFS      AS S
Sbjct: 4   MMEGVVVKLPSF------PLLNPT------IPPLSSSSLPL-RLGFRRFS------ASLS 63

Query: 61  TAKETDSLHLNSLSLGHTTRPDFPILHQEINGSKLVYLDNAATSQKPISVLNALQNYYEA 120
             KE       S+SLGH +RPDFPILHQE+NGSKLVYLDNAATSQKP +VL AL NYYEA
Sbjct: 64  AVKE------GSVSLGHLSRPDFPILHQEVNGSKLVYLDNAATSQKPTAVLKALHNYYEA 123

Query: 121 YNSNVHRGIHSLSAKATDEYERARKKVASFINAGDAKEIIFTRNATEAINLVAYSWGLAN 180
           YNSNVHRGIH LSAKATDEYE ARKKVA+FINA D+ EI+FTRNATEAINLVAYSWGL N
Sbjct: 124 YNSNVHRGIHYLSAKATDEYELARKKVAAFINASDSSEIVFTRNATEAINLVAYSWGLLN 183

Query: 181 LKSEDEIILTVAEHHSAIVPWQLVAERTGAVLKFVSLGEHDVPNLKDFKEMFSTKTKLVV 240
           LK  DEI+LT+AEHHSAIVPWQ+VA++TGAVLKFVSL E+++PN+   +EM ST+T+LVV
Sbjct: 184 LKPGDEIVLTIAEHHSAIVPWQIVAQKTGAVLKFVSLDENEIPNVVKLREMISTRTELVV 243

Query: 241 THHVSNVLASVLPIEEIVGLAHRFGAKVLVDACQSVPHMVVNVQALDADFLVASSHK 298
            HHVSNVL SVLPIEEIV   H  GAKVLVDACQSVPHMVV+VQ LDADFLVASSHK
Sbjct: 244 VHHVSNVLGSVLPIEEIVHWVHEVGAKVLVDACQSVPHMVVDVQGLDADFLVASSHK 275

BLAST of MELO3C014533 vs. TrEMBL
Match: A0A067KRV2_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_11109 PE=4 SV=1)

HSP 1 Score: 386.3 bits (991), Expect = 3.5e-104
Identity = 205/301 (68.11%), Postives = 239/301 (79.40%), Query Frame = 1

Query: 2   MEGMVLKLPSFHSQFQFQLMNPNRCISCFSSTLRASSSPL-LRFGNPRFSSPLS----VS 61
           M+G V KLPSF      + + PN      SS    + SP+ +R G  R SS  S    + 
Sbjct: 1   MDGAVFKLPSF------KFLRPN------SSPPATTKSPVVIRCGLTRLSSSYSSSSAIQ 60

Query: 62  ASASTAKETDSLHLNSLSLGHTTRPDFPILHQEINGSKLVYLDNAATSQKPISVLNALQN 121
           A A+T +E D   L SLSLGH TRPDFPILHQE+NGSKLVYLDNAATSQKP SVL ALQN
Sbjct: 61  AIATTGREGDQA-LGSLSLGHITRPDFPILHQEVNGSKLVYLDNAATSQKPNSVLRALQN 120

Query: 122 YYEAYNSNVHRGIHSLSAKATDEYERARKKVASFINAGDAKEIIFTRNATEAINLVAYSW 181
           YYE YNSNVHRGIH LSAKATDEYE ARKKVA+FINA D++EI+FTRNATEAINLVAYSW
Sbjct: 121 YYETYNSNVHRGIHYLSAKATDEYELARKKVAAFINASDSREIVFTRNATEAINLVAYSW 180

Query: 182 GLANLKSEDEIILTVAEHHSAIVPWQLVAERTGAVLKFVSLGEHDVPNLKDFKEMFSTKT 241
           GLANLK  DE+++T+AEHHSAIVPWQLVA+RTGA+LKFV+L E++VP+++  KE+ S  T
Sbjct: 181 GLANLKPGDEVVVTIAEHHSAIVPWQLVAQRTGALLKFVNLNENEVPDVQQLKEVISQNT 240

Query: 242 KLVVTHHVSNVLASVLPIEEIVGLAHRFGAKVLVDACQSVPHMVVNVQALDADFLVASSH 298
           +LVV HH+SNVLAS LPIE+I+  AH  GAKVL+DACQSVPHMVV+VQ L+ADFLVASSH
Sbjct: 241 RLVVVHHISNVLASFLPIEDIIEWAHDAGAKVLIDACQSVPHMVVDVQRLNADFLVASSH 288

BLAST of MELO3C014533 vs. TrEMBL
Match: A0A0D2W9F3_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_013G254300 PE=4 SV=1)

HSP 1 Score: 383.3 bits (983), Expect = 2.9e-103
Identity = 203/296 (68.58%), Postives = 232/296 (78.38%), Query Frame = 1

Query: 2   MEGMVLKLPSFHSQFQFQLMNPNRCISCFSSTLRASSSPLLRFGNPRFSSPLSVSASAST 61
           MEG+V+KLPSF        ++PN   S  SST R         G  RFS+ +S + +   
Sbjct: 1   MEGLVVKLPSF------PFLSPNISSSSSSSTFR--------LGFRRFSASISAAVNEGF 60

Query: 62  AKETDSLHLNSLSLGHTTRPDFPILHQEINGSKLVYLDNAATSQKPISVLNALQNYYEAY 121
                      +SLG  TRPDFPILHQE+NGSKLVYLDNAATSQKP +VL +LQNYYEAY
Sbjct: 61  -----------VSLGQLTRPDFPILHQEVNGSKLVYLDNAATSQKPTAVLKSLQNYYEAY 120

Query: 122 NSNVHRGIHSLSAKATDEYERARKKVASFINAGDAKEIIFTRNATEAINLVAYSWGLANL 181
           NSNVHRGIH LSAKATDEYE ARKKVA+FINA D+ EI+FTRNATEAINLVAYSWGL+NL
Sbjct: 121 NSNVHRGIHYLSAKATDEYELARKKVAAFINASDSAEIVFTRNATEAINLVAYSWGLSNL 180

Query: 182 KSEDEIILTVAEHHSAIVPWQLVAERTGAVLKFVSLGEHDVPNLKDFKEMFSTKTKLVVT 241
           K  DEIILT+AEHHSAIVPWQ+VA++TGA+LKFVSL E++VP+++  +EM ST TKLVV 
Sbjct: 181 KPRDEIILTIAEHHSAIVPWQIVAQKTGAILKFVSLDENEVPDVEKLREMISTSTKLVVV 240

Query: 242 HHVSNVLASVLPIEEIVGLAHRFGAKVLVDACQSVPHMVVNVQALDADFLVASSHK 298
           HHVSNVL SVLPIE+IV  AH  GAKVLVDACQSVPHMVV+VQ LDADFLVASSHK
Sbjct: 241 HHVSNVLGSVLPIEDIVIWAHAVGAKVLVDACQSVPHMVVDVQGLDADFLVASSHK 271

BLAST of MELO3C014533 vs. TAIR10
Match: AT1G08490.1 (AT1G08490.1 chloroplastic NIFS-like cysteine desulfurase)

HSP 1 Score: 367.9 bits (943), Expect = 6.5e-102
Identity = 197/296 (66.55%), Postives = 224/296 (75.68%), Query Frame = 1

Query: 2   MEGMVLKLPSFHSQFQFQLMNPNRCISCFSSTLRASSSPLLRFGNPRFSSPLSVSASAST 61
           MEG+ +KLPSF          PN           A S     F   R SS LSV ++A+ 
Sbjct: 1   MEGVAMKLPSF----------PN-----------AISIGHRSFSRVRCSSSLSVCSAAAA 60

Query: 62  AKETDSLHLNSLSLGHTTRPDFPILHQEINGSKLVYLDNAATSQKPISVLNALQNYYEAY 121
           +  T S    S+SLGH  R DF ILHQE+NGSKLVYLD+AATSQKP +VL+ALQNYYE Y
Sbjct: 61  SSATISTDSESVSLGHRVRKDFRILHQEVNGSKLVYLDSAATSQKPAAVLDALQNYYEFY 120

Query: 122 NSNVHRGIHSLSAKATDEYERARKKVASFINAGDAKEIIFTRNATEAINLVAYSWGLANL 181
           NSNVHRGIH LSAKATDE+E ARKKVA FINA D++EI+FTRNATEAINLVAYSWGL+NL
Sbjct: 121 NSNVHRGIHYLSAKATDEFELARKKVARFINASDSREIVFTRNATEAINLVAYSWGLSNL 180

Query: 182 KSEDEIILTVAEHHSAIVPWQLVAERTGAVLKFVSLGEHDVPNLKDFKEMFSTKTKLVVT 241
           K  DE+ILTVAEHHS IVPWQ+V+++TGAVLKFV+L E +VP++   +E+ S KTKLV  
Sbjct: 181 KPGDEVILTVAEHHSCIVPWQIVSQKTGAVLKFVTLNEDEVPDINKLRELISPKTKLVAV 240

Query: 242 HHVSNVLASVLPIEEIVGLAHRFGAKVLVDACQSVPHMVVNVQALDADFLVASSHK 298
           HHVSNVLAS LPIEEIV  AH  GAKVLVDACQSVPHMVV+VQ L+ADFLVASSHK
Sbjct: 241 HHVSNVLASSLPIEEIVVWAHDVGAKVLVDACQSVPHMVVDVQKLNADFLVASSHK 275

BLAST of MELO3C014533 vs. NCBI nr
Match: gi|659097418|ref|XP_008449615.1| (PREDICTED: cysteine desulfurase 2, chloroplastic-like [Cucumis melo])

HSP 1 Score: 584.3 bits (1505), Expect = 1.2e-163
Identity = 297/297 (100.00%), Postives = 297/297 (100.00%), Query Frame = 1

Query: 1   MMEGMVLKLPSFHSQFQFQLMNPNRCISCFSSTLRASSSPLLRFGNPRFSSPLSVSASAS 60
           MMEGMVLKLPSFHSQFQFQLMNPNRCISCFSSTLRASSSPLLRFGNPRFSSPLSVSASAS
Sbjct: 1   MMEGMVLKLPSFHSQFQFQLMNPNRCISCFSSTLRASSSPLLRFGNPRFSSPLSVSASAS 60

Query: 61  TAKETDSLHLNSLSLGHTTRPDFPILHQEINGSKLVYLDNAATSQKPISVLNALQNYYEA 120
           TAKETDSLHLNSLSLGHTTRPDFPILHQEINGSKLVYLDNAATSQKPISVLNALQNYYEA
Sbjct: 61  TAKETDSLHLNSLSLGHTTRPDFPILHQEINGSKLVYLDNAATSQKPISVLNALQNYYEA 120

Query: 121 YNSNVHRGIHSLSAKATDEYERARKKVASFINAGDAKEIIFTRNATEAINLVAYSWGLAN 180
           YNSNVHRGIHSLSAKATDEYERARKKVASFINAGDAKEIIFTRNATEAINLVAYSWGLAN
Sbjct: 121 YNSNVHRGIHSLSAKATDEYERARKKVASFINAGDAKEIIFTRNATEAINLVAYSWGLAN 180

Query: 181 LKSEDEIILTVAEHHSAIVPWQLVAERTGAVLKFVSLGEHDVPNLKDFKEMFSTKTKLVV 240
           LKSEDEIILTVAEHHSAIVPWQLVAERTGAVLKFVSLGEHDVPNLKDFKEMFSTKTKLVV
Sbjct: 181 LKSEDEIILTVAEHHSAIVPWQLVAERTGAVLKFVSLGEHDVPNLKDFKEMFSTKTKLVV 240

Query: 241 THHVSNVLASVLPIEEIVGLAHRFGAKVLVDACQSVPHMVVNVQALDADFLVASSHK 298
           THHVSNVLASVLPIEEIVGLAHRFGAKVLVDACQSVPHMVVNVQALDADFLVASSHK
Sbjct: 241 THHVSNVLASVLPIEEIVGLAHRFGAKVLVDACQSVPHMVVNVQALDADFLVASSHK 297

BLAST of MELO3C014533 vs. NCBI nr
Match: gi|778716806|ref|XP_004140178.2| (PREDICTED: cysteine desulfurase 1, chloroplastic [Cucumis sativus])

HSP 1 Score: 551.2 bits (1419), Expect = 1.2e-153
Identity = 280/297 (94.28%), Postives = 285/297 (95.96%), Query Frame = 1

Query: 1   MMEGMVLKLPSFHSQFQFQLMNPNRCISCFSSTLRASSSPLLRFGNPRFSSPLSVSASAS 60
           MMEGMVLK PSFHSQF FQ MNPNRCISCFSSTLRASSSPLLRF NPR  S  SVSASAS
Sbjct: 30  MMEGMVLKSPSFHSQFHFQFMNPNRCISCFSSTLRASSSPLLRFRNPRLPSHFSVSASAS 89

Query: 61  TAKETDSLHLNSLSLGHTTRPDFPILHQEINGSKLVYLDNAATSQKPISVLNALQNYYEA 120
           TAKETDSLHLNSLSLGHTTRPDFPILHQE+NGSKLVYLDNAATSQKPISVLNALQNYY+A
Sbjct: 90  TAKETDSLHLNSLSLGHTTRPDFPILHQEVNGSKLVYLDNAATSQKPISVLNALQNYYQA 149

Query: 121 YNSNVHRGIHSLSAKATDEYERARKKVASFINAGDAKEIIFTRNATEAINLVAYSWGLAN 180
           YNSNVHRGIH LSAKATDEYERARKKVASFINAGDAKEI+FTRNATEAINLVAYSWGLAN
Sbjct: 150 YNSNVHRGIHFLSAKATDEYERARKKVASFINAGDAKEIVFTRNATEAINLVAYSWGLAN 209

Query: 181 LKSEDEIILTVAEHHSAIVPWQLVAERTGAVLKFVSLGEHDVPNLKDFKEMFSTKTKLVV 240
           LKS DEIILTVAEHHSAIVPWQLVAERTGAVLKFV+LGEHDVPNLKD KEMFSTKTKLVV
Sbjct: 210 LKSGDEIILTVAEHHSAIVPWQLVAERTGAVLKFVNLGEHDVPNLKDLKEMFSTKTKLVV 269

Query: 241 THHVSNVLASVLPIEEIVGLAHRFGAKVLVDACQSVPHMVVNVQALDADFLVASSHK 298
           THHVSNVLASVLPI EIVGLAHRFGAKVLVDACQSVPHMVV+VQALDADFLVASSHK
Sbjct: 270 THHVSNVLASVLPIGEIVGLAHRFGAKVLVDACQSVPHMVVDVQALDADFLVASSHK 326

BLAST of MELO3C014533 vs. NCBI nr
Match: gi|590585006|ref|XP_007015333.1| (Chloroplastic NIFS-like cysteine desulfurase isoform 1 [Theobroma cacao])

HSP 1 Score: 388.7 bits (997), Expect = 1.0e-104
Identity = 210/297 (70.71%), Postives = 234/297 (78.79%), Query Frame = 1

Query: 1   MMEGMVLKLPSFHSQFQFQLMNPNRCISCFSSTLRASSSPLLRFGNPRFSSPLSVSASAS 60
           MMEG+V+KLPSF       L+NP          L +SS PL R G  RFS      AS S
Sbjct: 4   MMEGVVVKLPSF------PLLNPT------IPPLSSSSLPL-RLGFRRFS------ASLS 63

Query: 61  TAKETDSLHLNSLSLGHTTRPDFPILHQEINGSKLVYLDNAATSQKPISVLNALQNYYEA 120
             KE       S+SLGH +RPDFPILHQE+NGSKLVYLDNAATSQKP +VL AL NYYEA
Sbjct: 64  AVKE------GSVSLGHLSRPDFPILHQEVNGSKLVYLDNAATSQKPTAVLKALHNYYEA 123

Query: 121 YNSNVHRGIHSLSAKATDEYERARKKVASFINAGDAKEIIFTRNATEAINLVAYSWGLAN 180
           YNSNVHRGIH LSAKATDEYE ARKKVA+FINA D+ EI+FTRNATEAINLVAYSWGL N
Sbjct: 124 YNSNVHRGIHYLSAKATDEYELARKKVAAFINASDSSEIVFTRNATEAINLVAYSWGLLN 183

Query: 181 LKSEDEIILTVAEHHSAIVPWQLVAERTGAVLKFVSLGEHDVPNLKDFKEMFSTKTKLVV 240
           LK  DEI+LT+AEHHSAIVPWQ+VA++TGAVLKFVSL E+++PN+   +EM ST+T+LVV
Sbjct: 184 LKPGDEIVLTIAEHHSAIVPWQIVAQKTGAVLKFVSLDENEIPNVVKLREMISTRTELVV 243

Query: 241 THHVSNVLASVLPIEEIVGLAHRFGAKVLVDACQSVPHMVVNVQALDADFLVASSHK 298
            HHVSNVL SVLPIEEIV   H  GAKVLVDACQSVPHMVV+VQ LDADFLVASSHK
Sbjct: 244 VHHVSNVLGSVLPIEEIVHWVHEVGAKVLVDACQSVPHMVVDVQGLDADFLVASSHK 275

BLAST of MELO3C014533 vs. NCBI nr
Match: gi|590585009|ref|XP_007015334.1| (Chloroplastic NIFS-like cysteine desulfurase isoform 2 [Theobroma cacao])

HSP 1 Score: 388.7 bits (997), Expect = 1.0e-104
Identity = 210/297 (70.71%), Postives = 234/297 (78.79%), Query Frame = 1

Query: 1   MMEGMVLKLPSFHSQFQFQLMNPNRCISCFSSTLRASSSPLLRFGNPRFSSPLSVSASAS 60
           MMEG+V+KLPSF       L+NP          L +SS PL R G  RFS      AS S
Sbjct: 4   MMEGVVVKLPSF------PLLNPT------IPPLSSSSLPL-RLGFRRFS------ASLS 63

Query: 61  TAKETDSLHLNSLSLGHTTRPDFPILHQEINGSKLVYLDNAATSQKPISVLNALQNYYEA 120
             KE       S+SLGH +RPDFPILHQE+NGSKLVYLDNAATSQKP +VL AL NYYEA
Sbjct: 64  AVKE------GSVSLGHLSRPDFPILHQEVNGSKLVYLDNAATSQKPTAVLKALHNYYEA 123

Query: 121 YNSNVHRGIHSLSAKATDEYERARKKVASFINAGDAKEIIFTRNATEAINLVAYSWGLAN 180
           YNSNVHRGIH LSAKATDEYE ARKKVA+FINA D+ EI+FTRNATEAINLVAYSWGL N
Sbjct: 124 YNSNVHRGIHYLSAKATDEYELARKKVAAFINASDSSEIVFTRNATEAINLVAYSWGLLN 183

Query: 181 LKSEDEIILTVAEHHSAIVPWQLVAERTGAVLKFVSLGEHDVPNLKDFKEMFSTKTKLVV 240
           LK  DEI+LT+AEHHSAIVPWQ+VA++TGAVLKFVSL E+++PN+   +EM ST+T+LVV
Sbjct: 184 LKPGDEIVLTIAEHHSAIVPWQIVAQKTGAVLKFVSLDENEIPNVVKLREMISTRTELVV 243

Query: 241 THHVSNVLASVLPIEEIVGLAHRFGAKVLVDACQSVPHMVVNVQALDADFLVASSHK 298
            HHVSNVL SVLPIEEIV   H  GAKVLVDACQSVPHMVV+VQ LDADFLVASSHK
Sbjct: 244 VHHVSNVLGSVLPIEEIVHWVHEVGAKVLVDACQSVPHMVVDVQGLDADFLVASSHK 275

BLAST of MELO3C014533 vs. NCBI nr
Match: gi|802621477|ref|XP_012076008.1| (PREDICTED: cysteine desulfurase 1, chloroplastic isoform X1 [Jatropha curcas])

HSP 1 Score: 388.3 bits (996), Expect = 1.3e-104
Identity = 206/302 (68.21%), Postives = 240/302 (79.47%), Query Frame = 1

Query: 1   MMEGMVLKLPSFHSQFQFQLMNPNRCISCFSSTLRASSSPL-LRFGNPRFSSPLS----V 60
           MM+G V KLPSF      + + PN      SS    + SP+ +R G  R SS  S    +
Sbjct: 3   MMDGAVFKLPSF------KFLRPN------SSPPATTKSPVVIRCGLTRLSSSYSSSSAI 62

Query: 61  SASASTAKETDSLHLNSLSLGHTTRPDFPILHQEINGSKLVYLDNAATSQKPISVLNALQ 120
            A A+T +E D   L SLSLGH TRPDFPILHQE+NGSKLVYLDNAATSQKP SVL ALQ
Sbjct: 63  QAIATTGREGDQA-LGSLSLGHITRPDFPILHQEVNGSKLVYLDNAATSQKPNSVLRALQ 122

Query: 121 NYYEAYNSNVHRGIHSLSAKATDEYERARKKVASFINAGDAKEIIFTRNATEAINLVAYS 180
           NYYE YNSNVHRGIH LSAKATDEYE ARKKVA+FINA D++EI+FTRNATEAINLVAYS
Sbjct: 123 NYYETYNSNVHRGIHYLSAKATDEYELARKKVAAFINASDSREIVFTRNATEAINLVAYS 182

Query: 181 WGLANLKSEDEIILTVAEHHSAIVPWQLVAERTGAVLKFVSLGEHDVPNLKDFKEMFSTK 240
           WGLANLK  DE+++T+AEHHSAIVPWQLVA+RTGA+LKFV+L E++VP+++  KE+ S  
Sbjct: 183 WGLANLKPGDEVVVTIAEHHSAIVPWQLVAQRTGALLKFVNLNENEVPDVQQLKEVISQN 242

Query: 241 TKLVVTHHVSNVLASVLPIEEIVGLAHRFGAKVLVDACQSVPHMVVNVQALDADFLVASS 298
           T+LVV HH+SNVLAS LPIE+I+  AH  GAKVL+DACQSVPHMVV+VQ L+ADFLVASS
Sbjct: 243 TRLVVVHHISNVLASFLPIEDIIEWAHDAGAKVLIDACQSVPHMVVDVQRLNADFLVASS 291

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
CNIF1_ARATH1.1e-10066.55Cysteine desulfurase 1, chloroplastic OS=Arabidopsis thaliana GN=NFS2 PE=1 SV=1[more]
CSD_SYNY32.0e-7665.18Probable cysteine desulfurase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) GN... [more]
CSD_BACHD1.5e-6354.75Probable cysteine desulfurase OS=Bacillus halodurans (strain ATCC BAA-125 / DSM ... [more]
SUFS_BACSU9.5e-6353.21Cysteine desulfurase SufS OS=Bacillus subtilis (strain 168) GN=sufS PE=1 SV=1[more]
CSD_MYCPA1.4e-5849.08Probable cysteine desulfurase OS=Mycobacterium paratuberculosis (strain ATCC BAA... [more]
Match NameE-valueIdentityDescription
A0A0A0KEG9_CUCSA8.2e-15494.28Uncharacterized protein OS=Cucumis sativus GN=Csa_6G427980 PE=4 SV=1[more]
A0A061GU52_THECC7.0e-10570.71Chloroplastic NIFS-like cysteine desulfurase isoform 2 OS=Theobroma cacao GN=TCM... [more]
A0A061GUT7_THECC7.0e-10570.71Chloroplastic NIFS-like cysteine desulfurase isoform 1 OS=Theobroma cacao GN=TCM... [more]
A0A067KRV2_JATCU3.5e-10468.11Uncharacterized protein OS=Jatropha curcas GN=JCGZ_11109 PE=4 SV=1[more]
A0A0D2W9F3_GOSRA2.9e-10368.58Uncharacterized protein OS=Gossypium raimondii GN=B456_013G254300 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT1G08490.16.5e-10266.55 chloroplastic NIFS-like cysteine desulfurase[more]
Match NameE-valueIdentityDescription
gi|659097418|ref|XP_008449615.1|1.2e-163100.00PREDICTED: cysteine desulfurase 2, chloroplastic-like [Cucumis melo][more]
gi|778716806|ref|XP_004140178.2|1.2e-15394.28PREDICTED: cysteine desulfurase 1, chloroplastic [Cucumis sativus][more]
gi|590585006|ref|XP_007015333.1|1.0e-10470.71Chloroplastic NIFS-like cysteine desulfurase isoform 1 [Theobroma cacao][more]
gi|590585009|ref|XP_007015334.1|1.0e-10470.71Chloroplastic NIFS-like cysteine desulfurase isoform 2 [Theobroma cacao][more]
gi|802621477|ref|XP_012076008.1|1.3e-10468.21PREDICTED: cysteine desulfurase 1, chloroplastic isoform X1 [Jatropha curcas][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000192Aminotrans_V_dom
IPR015421PyrdxlP-dep_Trfase_major
IPR015424PyrdxlP-dep_Trfase
IPR000192Aminotrans_V_dom
IPR010970Cys_dSase_SufS
IPR015421PyrdxlP-dep_Trfase_major
IPR015422PyrdxlP-dep_Trfase_dom1
IPR015424PyrdxlP-dep_Trfase
IPR000192Aminotrans_V_dom
IPR010970Cys_dSase_SufS
IPR015421PyrdxlP-dep_Trfase_major
IPR015422PyrdxlP-dep_Trfase_dom1
IPR015424PyrdxlP-dep_Trfase
Vocabulary: Molecular Function
TermDefinition
GO:0003824catalytic activity
GO:0030170pyridoxal phosphate binding
GO:0031071cysteine desulfurase activity
GO:0003824catalytic activity
GO:0030170pyridoxal phosphate binding
GO:0031071cysteine desulfurase activity
GO:0003824catalytic activity
Vocabulary: Biological Process
TermDefinition
GO:0006534cysteine metabolic process
GO:0006534cysteine metabolic process
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009073 aromatic amino acid family biosynthetic process
biological_process GO:0006534 cysteine metabolic process
biological_process GO:0008150 biological_process
biological_process GO:0006913 nucleocytoplasmic transport
biological_process GO:0055085 transmembrane transport
biological_process GO:0000394 RNA splicing, via endonucleolytic cleavage and ligation
biological_process GO:0009733 response to auxin
biological_process GO:0009909 regulation of flower development
biological_process GO:0006606 protein import into nucleus
biological_process GO:0006406 mRNA export from nucleus
biological_process GO:0009086 methionine biosynthetic process
biological_process GO:0010074 maintenance of meristem identity
biological_process GO:0009870 defense response signaling pathway, resistance gene-dependent
biological_process GO:0010039 response to iron ion
biological_process GO:0010035 response to inorganic substance
biological_process GO:0018283 iron incorporation into metallo-sulfur cluster
biological_process GO:0055072 iron ion homeostasis
biological_process GO:0001887 selenium compound metabolic process
biological_process GO:0010269 response to selenium ion
biological_process GO:0000302 response to reactive oxygen species
biological_process GO:0016226 iron-sulfur cluster assembly
cellular_component GO:0005643 nuclear pore
cellular_component GO:0031965 nuclear membrane
cellular_component GO:0005575 cellular_component
cellular_component GO:0009507 chloroplast
molecular_function GO:0009000 selenocysteine lyase activity
molecular_function GO:0031071 cysteine desulfurase activity
molecular_function GO:0015288 porin activity
molecular_function GO:0016829 lyase activity
molecular_function GO:0017056 structural constituent of nuclear pore
molecular_function GO:0003824 catalytic activity
molecular_function GO:0030170 pyridoxal phosphate binding
This gene is associated with the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
MU48593melon EST collection version 4.0transcribed_cluster
MU49969melon EST collection version 4.0transcribed_cluster

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MELO3C014533T3MELO3C014533T3mRNA
MELO3C014533T2MELO3C014533T2mRNA
MELO3C014533T1MELO3C014533T1mRNA


The following transcribed_cluster feature(s) are associated with this gene:

Feature NameUnique NameType
MU48593MU48593transcribed_cluster
MU49969MU49969transcribed_cluster


Analysis Name: InterPro Annotations of melon
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000192Aminotransferase class V domainPFAMPF00266Aminotran_5coord: 96..470
score: 4.5E
IPR010970Cysteine desulfurase, SufSTIGRFAMsTIGR01979TIGR01979coord: 79..481
score: 7.6E
IPR015421Pyridoxal phosphate-dependent transferase, major region, subdomain 1GENE3DG3DSA:3.40.640.10coord: 81..329
score: 1.1
IPR015422Pyridoxal phosphate-dependent transferase, major region, subdomain 2GENE3DG3DSA:3.90.1150.10coord: 330..485
score: 1.4
IPR015424Pyridoxal phosphate-dependent transferaseunknownSSF53383PLP-dependent transferasescoord: 79..477
score: 5.91E
NoneNo IPR availablePANTHERPTHR11601CYSTEINE DESULFURYLASEcoord: 1..485
score: 1.3E
NoneNo IPR availablePANTHERPTHR11601:SF17CYSTEINE DESULFURASE 2, CHLOROPLASTICcoord: 1..485
score: 1.3E