Lsi07G010670.1 (mRNA) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi07G010670.1
Type	mRNA
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Protein TIC 214
Location	chr07 : 16032075 .. 16033111 (-)
Sequence length	684

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTGTAACAATCCTAAAAAAAAGTTCCATTCGAGATTGGACTAAGAAAGGGAAGGAAGAAGGCGAGTCAGTATGCTAATGCCTCATCCTCAAATCAATCCTTCTCATAGGTTATTGTCCCAACGAATAAGTAATTGTAGAAGTGAAAGCTTCTTATAATTCGAAAAAGCAAGAGCAGCAAGTCCAAGTAAATAAAATACGAAAAAAAATACGTAATTTTTTTAGGATTAAACAAAAGGATTCGCAAATAAAAGTGCTAATGTTACAACCAGTCTATAAATTGTTAAAGCTTCCATAAAAGCCAGACTAAGCAATAAAGTACCTCAGATTTTTCCCTCCGCCTCAGGTTGTCTTGCGATCCCTTCTACAGCTTGGCCCGCAGCAGTACCTTGACCAACCCCAGGTCCAATAGAAGCAAGCCCGACGGCTGGAACAATCACAAACACCTTTTTGATTATGGATCTACTACCAGAAACTCAATGCGTAATCTTAGCATTCAATGTGTATTCCAGAATAATCTCATTTTTCAATTATTCAACCATTTCATTTTACCAAGTTCAATGTTAGTTAGATTAGTCAACATTTCTATGTTTCGATGCAACAACAAGATGTTATTTGTAACAAGTAGTTTTGTTGGTTGGTTAATTGGTCACATTTTATTCAAGAAATGGGTTGGATTGATATTAGTCTGGATCAGCAAAATAATTCTATTAGATCTAATAAGTACCTTGTGTCAGAATTGAGAAATTCTATGGCTCGAATCTTTAGTATTCTCTTATTTATTACCTGTGTCTACTATTTAGGCAGAATACCGTCACCCAGTGTTACTAAGAAACTGAAAGAAACGGAGAAAATGGAGGAAAGGGGGGAAAGTGAGGAAGAAACAGATGTAGAAATAGAAACCACTTCCGAAACGAAGGGGACTAAACAGGAACAAGAGGGATCCACCGAAGAAGATACTTCTTCCGAAAAAAGACTTTGTAGTTATAATACCCAAATACCAAGTCGGTTCATTCATATTTATATTGATCATTAG

mRNA sequence

ATGTTGTTGTCTTGCGATCCCTTCTACAGCTTGGCCCGCAGCAGTACCTTGACCAACCCCAGGTCCAATAGAAGCAAGCCCGACGGCTGGAACAATCACAAACACCTTTTTGATTATGGATCTACTACCAGAAACTCAATGCGTAATCTTAGCATTCAATGTGTATTCCAGAATAATCTCATTTTTCAATTATTCAACCATTTCATTTTACCAAGTTCAATGTTAGTTAGATTAGTCAACATTTCTATGTTTCGATGCAACAACAAGATGTTATTTTTTTGTTGGTTGGTTAATTGGTCACATTTTATTCAAGAAATGGGTTGGATTGATATTAGTCTGGATCAGCAAAATAATTCTATTAGATCTAATAAGTACCTTGTGTCAGAATTGAGAAATTCTATGGCTCGAATCTTTAGTATTCTCTTATTTATTACCTGTGTCTACTATTTAGGCAGAATACCGTCACCCAGTGTTACTAAGAAACTGAAAGAAACGGAGAAAATGGAGGAAAGGGGGGAAAGTGAGGAAGAAACAGATGTAGAAATAGAAACCACTTCCGAAACGAAGGGGACTAAACAGGAACAAGAGGGATCCACCGAAGAAGATACTTCTTCCGAAAAAAGACTTTGTAGTTATAATACCCAAATACCAAGTCGGTTCATTCATATTTATATTGATCATTAG

Coding sequence (CDS)

ATGTTGTTGTCTTGCGATCCCTTCTACAGCTTGGCCCGCAGCAGTACCTTGACCAACCCCAGGTCCAATAGAAGCAAGCCCGACGGCTGGAACAATCACAAACACCTTTTTGATTATGGATCTACTACCAGAAACTCAATGCGTAATCTTAGCATTCAATGTGTATTCCAGAATAATCTCATTTTTCAATTATTCAACCATTTCATTTTACCAAGTTCAATGTTAGTTAGATTAGTCAACATTTCTATGTTTCGATGCAACAACAAGATGTTATTTTTTTGTTGGTTGGTTAATTGGTCACATTTTATTCAAGAAATGGGTTGGATTGATATTAGTCTGGATCAGCAAAATAATTCTATTAGATCTAATAAGTACCTTGTGTCAGAATTGAGAAATTCTATGGCTCGAATCTTTAGTATTCTCTTATTTATTACCTGTGTCTACTATTTAGGCAGAATACCGTCACCCAGTGTTACTAAGAAACTGAAAGAAACGGAGAAAATGGAGGAAAGGGGGGAAAGTGAGGAAGAAACAGATGTAGAAATAGAAACCACTTCCGAAACGAAGGGGACTAAACAGGAACAAGAGGGATCCACCGAAGAAGATACTTCTTCCGAAAAAAGACTTTGTAGTTATAATACCCAAATACCAAGTCGGTTCATTCATATTTATATTGATCATTAG

Protein sequence

MLLSCDPFYSLARSSTLTNPRSNRSKPDGWNNHKHLFDYGSTTRNSMRNLSIQCVFQNNLIFQLFNHFILPSSMLVRLVNISMFRCNNKMLFFCWLVNWSHFIQEMGWIDISLDQQNNSIRSNKYLVSELRNSMARIFSILLFITCVYYLGRIPSPSVTKKLKETEKMEERGESEEETDVEIETTSETKGTKQEQEGSTEEDTSSEKRLCSYNTQIPSRFIHIYIDH

Homology

[Close]

BLAST of Lsi07G010670.1 vs. Swiss-Prot
Match: TI214_DRIGR (Protein TIC 214 OS=Drimys granadensis GN=TIC214 PE=3 SV=1)

HSP 1 Score: 255.4 bits (651), Expect = 6.0e-67
Identity = 146/200 (73.00%), Postives = 157/200 (78.50%), Query Frame = 1


Query: 30  WNNHKHLFDYGSTTRNSMRNLSIQCVFQNNLIFQLFNHFILPSSMLVRLVNISMFRCNNK 89
           WNNHKH FDYGSTTRNSMRNLSIQCVF NNLIFQLFNHFILPSS L RLVNI MFRCNNK
Sbjct: 104 WNNHKHFFDYGSTTRNSMRNLSIQCVFLNNLIFQLFNHFILPSSTLARLVNIYMFRCNNK 163

Query: 90  MLFFC-----WLVNWSHFIQEMGWIDISLDQQNNS-----IRSNKYLVSELRNSMARIFS 149
           MLF       WL+    F++ +G +   + Q+N+      IRSNKYLVSELRNSMARIFS
Sbjct: 164 MLFVTSSFIGWLIGHILFMKWVGLVLFWIRQKNSIRSNVLIRSNKYLVSELRNSMARIFS 223

Query: 150 ILLFITCVYYLGRIPSPSVTKKLKETEKMEERGESEEETDVEIETTSETKGTKQEQEGST 209
           ILLFI CVYYLGRIPSP VTKKLKET + EERGESEEETDVEIETTSETKGTKQEQEGST
Sbjct: 224 ILLFIICVYYLGRIPSPIVTKKLKETSETEERGESEEETDVEIETTSETKGTKQEQEGST 283

Query: 210 EEDTSSEKRLCSYNTQIPSR 220
           EED S     CS   + P +
Sbjct: 284 EEDPS----FCSEEKEDPDK 299

[Close]

BLAST of Lsi07G010670.1 vs. Swiss-Prot
Match: TI214_EUCGG (Protein TIC 214 OS=Eucalyptus globulus subsp. globulus GN=TIC214 PE=3 SV=1)

HSP 1 Score: 255.0 bits (650), Expect = 7.8e-67
Identity = 146/188 (77.66%), Postives = 152/188 (80.85%), Query Frame = 1


Query: 30  WNNHKHLFDYGSTTRNSMRNLSIQCVFQNNLIFQLFNHFILPSSMLVRLVNISMFRCNNK 89
           WNNHKH FDYGSTTRNSMRNLSIQCVF NNLIFQLFNHFILPSSML RLVNI MFRCNNK
Sbjct: 107 WNNHKHFFDYGSTTRNSMRNLSIQCVFLNNLIFQLFNHFILPSSMLARLVNIYMFRCNNK 166

Query: 90  MLFFC-----WLVNWSHFIQEMGWIDISLD--QQNNS------IRSNKYLVSELRNSMAR 149
           MLF       WL+    F   M W+++ L   QQNNS      IRSNKYLVSELRNSMAR
Sbjct: 167 MLFVTSSFVGWLIGHIFF---MKWVELVLVWIQQNNSIRSNKYIRSNKYLVSELRNSMAR 226

Query: 150 IFSILLFITCVYYLGRIPSPSVTKKLKETEKMEERGESEEETDVEIETTSETKGTKQEQE 205
           IFSILLFITCVYYLGRIPSP VTKK KET + EERGE EEETDVEIETT ETKGT+QEQE
Sbjct: 227 IFSILLFITCVYYLGRIPSPIVTKKFKETSETEERGEGEEETDVEIETTFETKGTRQEQE 286

[Close]

BLAST of Lsi07G010670.1 vs. Swiss-Prot
Match: TI214_LIRTU (Protein TIC 214 OS=Liriodendron tulipifera GN=TIC214 PE=3 SV=1)

HSP 1 Score: 253.4 bits (646), Expect = 2.3e-66
Identity = 146/200 (73.00%), Postives = 155/200 (77.50%), Query Frame = 1


Query: 30  WNNHKHLFDYGSTTRNSMRNLSIQCVFQNNLIFQLFNHFILPSSMLVRLVNISMFRCNNK 89
           WNNHKH FDYGSTTRNSMRNLSIQCVF NNLIFQLFNHFILPSS L RLVNI MFRCNNK
Sbjct: 104 WNNHKHFFDYGSTTRNSMRNLSIQCVFLNNLIFQLFNHFILPSSTLARLVNIYMFRCNNK 163

Query: 90  MLFFC-----WLVNWSHFIQEMGWIDISLDQQ-----NNSIRSNKYLVSELRNSMARIFS 149
           MLF       WL+    F++ +G +   + Q      N  IRSNKYLVSELRNSMARIFS
Sbjct: 164 MLFVTSSFVGWLIGHIFFMKWVGLVLFWIRQNHSIRSNVLIRSNKYLVSELRNSMARIFS 223

Query: 150 ILLFITCVYYLGRIPSPSVTKKLKETEKMEERGESEEETDVEIETTSETKGTKQEQEGST 209
           ILLFITCVYYLGRIPSP VTKKLKET + EERGES EETDVEIETTSETKGTKQEQE ST
Sbjct: 224 ILLFITCVYYLGRIPSPIVTKKLKETSETEERGESAEETDVEIETTSETKGTKQEQEAST 283

Query: 210 EEDTSSEKRLCSYNTQIPSR 220
           EED S    LCS   + P +
Sbjct: 284 EEDPS----LCSEEKEDPDK 299

[Close]

BLAST of Lsi07G010670.1 vs. Swiss-Prot
Match: TI214_BUXMI (Protein TIC 214 OS=Buxus microphylla GN=TIC214 PE=3 SV=1)

HSP 1 Score: 251.1 bits (640), Expect = 1.1e-65
Identity = 145/200 (72.50%), Postives = 155/200 (77.50%), Query Frame = 1


Query: 30  WNNHKHLFDYGSTTRNSMRNLSIQCVFQNNLIFQLFNHFILPSSMLVRLVNISMFRCNNK 89
           WNNHKH FDYGSTTRNSMRNLSIQCVF NNLIFQLFNHFILPSS L RLVNI MFRCNNK
Sbjct: 104 WNNHKHFFDYGSTTRNSMRNLSIQCVFLNNLIFQLFNHFILPSSTLARLVNIYMFRCNNK 163

Query: 90  MLFFC-----WLVNWSHFIQEMGWIDISLDQQ-----NNSIRSNKYLVSELRNSMARIFS 149
           MLF       WL+    F++ +G +   + Q      N  IRSNKYLVSELRNSMARIFS
Sbjct: 164 MLFVTSSFVGWLIGHILFMKWVGLVLFWIRQNHSIKSNVLIRSNKYLVSELRNSMARIFS 223

Query: 150 ILLFITCVYYLGRIPSPSVTKKLKETEKMEERGESEEETDVEIETTSETKGTKQEQEGST 209
           ILLFITCVYYLGR+P+P VTKKLKET K EERGESEEE DVEIETTSETKGTKQEQE ST
Sbjct: 224 ILLFITCVYYLGRMPAPIVTKKLKETSKTEERGESEEERDVEIETTSETKGTKQEQERST 283

Query: 210 EEDTSSEKRLCSYNTQIPSR 220
           EED S    LCS   + P +
Sbjct: 284 EEDPSPS--LCSEEKEDPDK 301

[Close]

BLAST of Lsi07G010670.1 vs. Swiss-Prot
Match: T214A_PIPCE (Putative protein TIC 214 N-terminal part OS=Piper cenocladum GN=TIC214 PE=5 SV=1)

HSP 1 Score: 250.8 bits (639), Expect = 1.5e-65
Identity = 143/195 (73.33%), Postives = 158/195 (81.03%), Query Frame = 1


Query: 30  WNNHKHLFDYGSTTRNSMRNLSIQCVFQNNLIFQLFNHFILPSSMLVRLVNISMFRCNNK 89
           WNNHKH  DYGSTTRNSMRNLSIQCVF NNLIFQLFNHFILPSS L RLVNI +FRCN+K
Sbjct: 104 WNNHKHFLDYGSTTRNSMRNLSIQCVFLNNLIFQLFNHFILPSSTLARLVNIYLFRCNSK 163

Query: 90  MLFFC-----WLVNWSHFIQEMGWIDISLDQQNNSIRSNKYLVSELRNSMARIFSILLFI 149
           +LF       WL+    F++ +G + +S  +QN+SIRSNKYLVSEL NSMARIFSILLFI
Sbjct: 164 ILFVTSSFVGWLIGHILFMKWVGLV-LSWIRQNHSIRSNKYLVSELTNSMARIFSILLFI 223

Query: 150 TCVYYLGRIPSPSVTKKLKETEKMEERGESEEETDVEIETTSETKGTKQEQEGSTEEDTS 209
           T VYYLGR+PSP VTKKLKET +MEERGESEEETDVEIETTSETK TKQEQEGSTEED S
Sbjct: 224 TSVYYLGRMPSPIVTKKLKETSEMEERGESEEETDVEIETTSETKETKQEQEGSTEEDPS 283

Query: 210 SEKRLCSYNTQIPSR 220
               LCS   + P +
Sbjct: 284 ----LCSEEQEDPDK 293

[Close]

BLAST of Lsi07G010670.1 vs. TrEMBL
Match: A0A0K1NPU1_9MYRT (Hypothetical chloroplast RF19 OS=Eugenia uniflora GN=ycf1 PE=4 SV=1)

HSP 1 Score: 264.2 bits (674), Expect = 1.4e-67
Identity = 148/182 (81.32%), Postives = 153/182 (84.07%), Query Frame = 1


Query: 30  WNNHKHLFDYGSTTRNSMRNLSIQCVFQNNLIFQLFNHFILPSSMLVRLVNISMFRCNNK 89
           WNNHKH FDYGSTTRNSMRNLSIQCVF NNLIFQLFNHFILPSSML RLVNI MFRCNNK
Sbjct: 107 WNNHKHFFDYGSTTRNSMRNLSIQCVFLNNLIFQLFNHFILPSSMLARLVNIYMFRCNNK 166

Query: 90  MLFFC-----WLVNWSHFIQEMGWIDISLD--QQNNSIRSNKYLVSELRNSMARIFSILL 149
           MLF       WL+    F   M W+++ L   QQ NSIRSNKYLVSELRNSMARIFSILL
Sbjct: 167 MLFVTSSFVGWLIGHIFF---MKWVELVLVWIQQKNSIRSNKYLVSELRNSMARIFSILL 226

Query: 150 FITCVYYLGRIPSPSVTKKLKETEKMEERGESEEETDVEIETTSETKGTKQEQEGSTEED 205
           FITCVYYLGRIPSP VTKK KET +MEERGESEEETDVEIETT ETKGTKQEQEGSTEED
Sbjct: 227 FITCVYYLGRIPSPIVTKKFKETSEMEERGESEEETDVEIETTFETKGTKQEQEGSTEED 285

[Close]

BLAST of Lsi07G010670.1 vs. TrEMBL
Match: A0A0A7LE95_9MAGN (Hypothetical chloroplast RF1 OS=Clematis fusca var. coreana GN=ycf1 PE=4 SV=1)

HSP 1 Score: 263.8 bits (673), Expect = 1.9e-67
Identity = 148/195 (75.90%), Postives = 159/195 (81.54%), Query Frame = 1


Query: 30  WNNHKHLFDYGSTTRNSMRNLSIQCVFQNNLIFQLFNHFILPSSMLVRLVNISMFRCNNK 89
           WNNHKH FDYGSTTRNSMRNLSIQCVF NNLIFQLFNHFILPSS L RLVNI MF+CNNK
Sbjct: 104 WNNHKHFFDYGSTTRNSMRNLSIQCVFLNNLIFQLFNHFILPSSTLARLVNIYMFQCNNK 163

Query: 90  MLFFC-----WLVNWSHFIQEMGWIDISLDQQNNSIRSNKYLVSELRNSMARIFSILLFI 149
           MLF       WL+    F++ +G I   + +QN+SIRSNKYLVSELRNSMARIFSILLFI
Sbjct: 164 MLFVTSSFVGWLIGHILFMKWVGLILFWI-RQNHSIRSNKYLVSELRNSMARIFSILLFI 223

Query: 150 TCVYYLGRIPSPSVTKKLKETEKMEERGESEEETDVEIETTSETKGTKQEQEGSTEEDTS 209
           TCVYYLGRIPSP VTKKLKET K EERG+SEEE DVEIETTSETKGTKQEQEGS EED S
Sbjct: 224 TCVYYLGRIPSPIVTKKLKETSKKEERGKSEEEIDVEIETTSETKGTKQEQEGSAEEDPS 283

Query: 210 SEKRLCSYNTQIPSR 220
           S   LCS   + P +
Sbjct: 284 SSLSLCSEEKEDPDK 297

[Close]

BLAST of Lsi07G010670.1 vs. TrEMBL
Match: A0A0H3Y307_9MAGN (Photosystem I assembly protein Ycf1 OS=Anemone patens GN=ycf1 PE=4 SV=1)

HSP 1 Score: 263.5 bits (672), Expect = 2.4e-67
Identity = 147/195 (75.38%), Postives = 159/195 (81.54%), Query Frame = 1


Query: 30  WNNHKHLFDYGSTTRNSMRNLSIQCVFQNNLIFQLFNHFILPSSMLVRLVNISMFRCNNK 89
           WNNHKH FDYGSTTRNSMRNLSIQCVF NNLIFQLFNHFILPSS L RLVNI MF+CNNK
Sbjct: 104 WNNHKHFFDYGSTTRNSMRNLSIQCVFLNNLIFQLFNHFILPSSTLARLVNIYMFQCNNK 163

Query: 90  MLFFC-----WLVNWSHFIQEMGWIDISLDQQNNSIRSNKYLVSELRNSMARIFSILLFI 149
           MLF       WL+    F++ +G +   + +QN+SIRSNKYLVSELRNSMARIFSILLFI
Sbjct: 164 MLFVTSSFVGWLIGHILFMKWVGLVLFWI-RQNHSIRSNKYLVSELRNSMARIFSILLFI 223

Query: 150 TCVYYLGRIPSPSVTKKLKETEKMEERGESEEETDVEIETTSETKGTKQEQEGSTEEDTS 209
           TCVYYLGRIPSP VTKKLKET K EERG+SEEE DVEIETTSETKGTKQEQEGS EED S
Sbjct: 224 TCVYYLGRIPSPIVTKKLKETSKKEERGKSEEEIDVEIETTSETKGTKQEQEGSAEEDPS 283

Query: 210 SEKRLCSYNTQIPSR 220
           S   LCS   + P +
Sbjct: 284 SSLSLCSEEKEDPDK 297

[Close]

BLAST of Lsi07G010670.1 vs. TrEMBL
Match: A0A0H3XZS7_9MAGN (Photosystem I assembly protein Ycf1 OS=Pulsatilla vernalis GN=ycf1 PE=4 SV=1)

HSP 1 Score: 263.5 bits (672), Expect = 2.4e-67
Identity = 147/195 (75.38%), Postives = 159/195 (81.54%), Query Frame = 1


Query: 30  WNNHKHLFDYGSTTRNSMRNLSIQCVFQNNLIFQLFNHFILPSSMLVRLVNISMFRCNNK 89
           WNNHKH FDYGSTTRNSMRNLSIQCVF NNLIFQLFNHFILPSS L RLVNI MF+CNNK
Sbjct: 104 WNNHKHFFDYGSTTRNSMRNLSIQCVFLNNLIFQLFNHFILPSSTLARLVNIYMFQCNNK 163

Query: 90  MLFFC-----WLVNWSHFIQEMGWIDISLDQQNNSIRSNKYLVSELRNSMARIFSILLFI 149
           MLF       WL+    F++ +G +   + +QN+SIRSNKYLVSELRNSMARIFSILLFI
Sbjct: 164 MLFVTSSFVGWLIGHILFMKWVGLVLFWI-RQNHSIRSNKYLVSELRNSMARIFSILLFI 223

Query: 150 TCVYYLGRIPSPSVTKKLKETEKMEERGESEEETDVEIETTSETKGTKQEQEGSTEEDTS 209
           TCVYYLGRIPSP VTKKLKET K EERG+SEEE DVEIETTSETKGTKQEQEGS EED S
Sbjct: 224 TCVYYLGRIPSPIVTKKLKETSKKEERGKSEEEIDVEIETTSETKGTKQEQEGSAEEDPS 283

Query: 210 SEKRLCSYNTQIPSR 220
           S   LCS   + P +
Sbjct: 284 SSLSLCSEEKEDPDK 297

[Close]

BLAST of Lsi07G010670.1 vs. TrEMBL
Match: A0A0N7BUX5_9ROSA (Hypothetical chloroplast RF19 OS=Elaeagnus macrophylla GN=ycf1 PE=4 SV=1)

HSP 1 Score: 263.5 bits (672), Expect = 2.4e-67
Identity = 146/180 (81.11%), Postives = 155/180 (86.11%), Query Frame = 1


Query: 30  WNNHKHLFDYGSTTRNSMRNLSIQCVFQNNLIFQLFNHFILPSSMLVRLVNISMFRCNNK 89
           WNNHKH FDYGST RNSMRNLSIQCVF NNLIFQLFNHFILPSSMLVRLVNI MFRCNNK
Sbjct: 104 WNNHKHFFDYGSTNRNSMRNLSIQCVFLNNLIFQLFNHFILPSSMLVRLVNIYMFRCNNK 163

Query: 90  MLFFC-----WLVNWSHFIQEMGWIDISLDQQNNSIRSNKYLVSELRNSMARIFSILLFI 149
           MLF       WL+    F++ +G + + + QQN+SIRSNKYLVSELRNSMARIFSILLFI
Sbjct: 164 MLFVTSSFVGWLIGHILFMKWVGLVLVWI-QQNHSIRSNKYLVSELRNSMARIFSILLFI 223

Query: 150 TCVYYLGRIPSPSVTKKLKETEKMEERGESEEETDVEIETTSETKGTKQEQEGSTEEDTS 205
           TCVYYLGRIPSP +TKKLKET K EERGESEEETDVEIETTSETK TKQEQEGSTEED S
Sbjct: 224 TCVYYLGRIPSPIITKKLKETSKTEERGESEEETDVEIETTSETKETKQEQEGSTEEDPS 282

[Close]

BLAST of Lsi07G010670.1 vs. TAIR10
Match: ATCG01130.1 (ATCG01130.1 Ycf1 protein)

HSP 1 Score: 202.6 bits (514), Expect = 2.6e-52
Identity = 121/185 (65.41%), Postives = 130/185 (70.27%), Query Frame = 1


Query: 30  WNNHKHLFDYGSTTRNSMRNLSIQCVFQNNLIFQLFNHFILPSSMLVRLVNISMFRCNNK 89
           WNNHKH FDYGSTTRN MRNL IQCVF NNLIFQLFNHFILPSSML RLVNI MFRCNNK
Sbjct: 108 WNNHKHFFDYGSTTRNEMRNLRIQCVFLNNLIFQLFNHFILPSSMLARLVNIYMFRCNNK 167

Query: 90  MLFFC-----WLVNWSHFIQEMGWIDISLDQQNNSIRSN--------KYLVSELRNSMAR 149
           MLF       WL+    F++ +G + + + QQNNSIRSN        K+LVSELRNSMAR
Sbjct: 168 MLFVTSSFVGWLIGHILFMKWVGLVLVWI-QQNNSIRSNVVIRSNKYKFLVSELRNSMAR 227

Query: 150 IFSILLFITCVYYLGRIPSPSVTKKLKETEKMEERGESEEETDVEIETTSETKGTKQEQE 202
           IFSILLFITCVYYLGRIPSP  TKKLK                     TSET GTKQ+QE
Sbjct: 228 IFSILLFITCVYYLGRIPSPIFTKKLK--------------------GTSETGGTKQDQE 271

[Close]

BLAST of Lsi07G010670.1 vs. TAIR10
Match: ATCG01000.1 (ATCG01000.1 Ycf1 protein)

HSP 1 Score: 202.6 bits (514), Expect = 2.6e-52
Identity = 121/185 (65.41%), Postives = 130/185 (70.27%), Query Frame = 1


Query: 30  WNNHKHLFDYGSTTRNSMRNLSIQCVFQNNLIFQLFNHFILPSSMLVRLVNISMFRCNNK 89
           WNNHKH FDYGSTTRN MRNL IQCVF NNLIFQLFNHFILPSSML RLVNI MFRCNNK
Sbjct: 108 WNNHKHFFDYGSTTRNEMRNLRIQCVFLNNLIFQLFNHFILPSSMLARLVNIYMFRCNNK 167

Query: 90  MLFFC-----WLVNWSHFIQEMGWIDISLDQQNNSIRSN--------KYLVSELRNSMAR 149
           MLF       WL+    F++ +G + + + QQNNSIRSN        K+LVSELRNSMAR
Sbjct: 168 MLFVTSSFVGWLIGHILFMKWVGLVLVWI-QQNNSIRSNVVIRSNKYKFLVSELRNSMAR 227

Query: 150 IFSILLFITCVYYLGRIPSPSVTKKLKETEKMEERGESEEETDVEIETTSETKGTKQEQE 202
           IFSILLFITCVYYLGRIPSP  TKKLK                     TSET GTKQ+QE
Sbjct: 228 IFSILLFITCVYYLGRIPSPIFTKKLK--------------------GTSETGGTKQDQE 271

[Close]

BLAST of Lsi07G010670.1 vs. TAIR10
Match: AT2G07739.1 (AT2G07739.1 Ycf1 protein)

HSP 1 Score: 116.7 bits (291), Expect = 1.9e-26
Identity = 55/63 (87.30%), Postives = 56/63 (88.89%), Query Frame = 1


Query: 30  WNNHKHLFDYGSTTRNSMRNLSIQCVFQNNLIFQLFNHFILPSSMLVRLVNISMFRCNNK 89
           WNNHKH FDYGSTTRN MRNL IQCVF NNLIF+LFNH ILPSSML RLVNI MFRCNNK
Sbjct: 108 WNNHKHFFDYGSTTRNEMRNLRIQCVFPNNLIFKLFNHLILPSSMLARLVNIYMFRCNNK 167

Query: 90  MLF 93
           MLF
Sbjct: 168 MLF 170

[Close]

BLAST of Lsi07G010670.1 vs. TAIR10
Match: ATMG00370.1 (ATMG00370.1 Ycf1 protein)

HSP 1 Score: 116.7 bits (291), Expect = 1.9e-26
Identity = 55/63 (87.30%), Postives = 56/63 (88.89%), Query Frame = 1


Query: 30  WNNHKHLFDYGSTTRNSMRNLSIQCVFQNNLIFQLFNHFILPSSMLVRLVNISMFRCNNK 89
           WNNHKH FDYGSTTRN MRNL IQCVF NNLIF+LFNH ILPSSML RLVNI MFRCNNK
Sbjct: 108 WNNHKHFFDYGSTTRNEMRNLRIQCVFPNNLIFKLFNHLILPSSMLARLVNIYMFRCNNK 167

Query: 90  MLF 93
           MLF
Sbjct: 168 MLF 170

[Close]

BLAST of Lsi07G010670.1 vs. NCBI nr
Match: gi|918021144|ref|YP_009163537.1| (hypothetical chloroplast RF19 (plastid) [Eugenia uniflora])

HSP 1 Score: 264.2 bits (674), Expect = 2.1e-67
Identity = 148/182 (81.32%), Postives = 153/182 (84.07%), Query Frame = 1


Query: 30  WNNHKHLFDYGSTTRNSMRNLSIQCVFQNNLIFQLFNHFILPSSMLVRLVNISMFRCNNK 89
           WNNHKH FDYGSTTRNSMRNLSIQCVF NNLIFQLFNHFILPSSML RLVNI MFRCNNK
Sbjct: 107 WNNHKHFFDYGSTTRNSMRNLSIQCVFLNNLIFQLFNHFILPSSMLARLVNIYMFRCNNK 166

Query: 90  MLFFC-----WLVNWSHFIQEMGWIDISLD--QQNNSIRSNKYLVSELRNSMARIFSILL 149
           MLF       WL+    F   M W+++ L   QQ NSIRSNKYLVSELRNSMARIFSILL
Sbjct: 167 MLFVTSSFVGWLIGHIFF---MKWVELVLVWIQQKNSIRSNKYLVSELRNSMARIFSILL 226

Query: 150 FITCVYYLGRIPSPSVTKKLKETEKMEERGESEEETDVEIETTSETKGTKQEQEGSTEED 205
           FITCVYYLGRIPSP VTKK KET +MEERGESEEETDVEIETT ETKGTKQEQEGSTEED
Sbjct: 227 FITCVYYLGRIPSPIVTKKFKETSEMEERGESEEETDVEIETTFETKGTKQEQEGSTEED 285

[Close]

BLAST of Lsi07G010670.1 vs. NCBI nr
Match: gi|731443691|gb|AIZ57585.1| (hypothetical chloroplast RF1 (chloroplast) [Clematis fusca var. coreana])

HSP 1 Score: 263.8 bits (673), Expect = 2.7e-67
Identity = 148/195 (75.90%), Postives = 159/195 (81.54%), Query Frame = 1


Query: 30  WNNHKHLFDYGSTTRNSMRNLSIQCVFQNNLIFQLFNHFILPSSMLVRLVNISMFRCNNK 89
           WNNHKH FDYGSTTRNSMRNLSIQCVF NNLIFQLFNHFILPSS L RLVNI MF+CNNK
Sbjct: 104 WNNHKHFFDYGSTTRNSMRNLSIQCVFLNNLIFQLFNHFILPSSTLARLVNIYMFQCNNK 163

Query: 90  MLFFC-----WLVNWSHFIQEMGWIDISLDQQNNSIRSNKYLVSELRNSMARIFSILLFI 149
           MLF       WL+    F++ +G I   + +QN+SIRSNKYLVSELRNSMARIFSILLFI
Sbjct: 164 MLFVTSSFVGWLIGHILFMKWVGLILFWI-RQNHSIRSNKYLVSELRNSMARIFSILLFI 223

Query: 150 TCVYYLGRIPSPSVTKKLKETEKMEERGESEEETDVEIETTSETKGTKQEQEGSTEEDTS 209
           TCVYYLGRIPSP VTKKLKET K EERG+SEEE DVEIETTSETKGTKQEQEGS EED S
Sbjct: 224 TCVYYLGRIPSPIVTKKLKETSKKEERGKSEEEIDVEIETTSETKGTKQEQEGSAEEDPS 283

Query: 210 SEKRLCSYNTQIPSR 220
           S   LCS   + P +
Sbjct: 284 SSLSLCSEEKEDPDK 297

[Close]

BLAST of Lsi07G010670.1 vs. NCBI nr
Match: gi|938339504|ref|YP_009171657.1| (hypothetical chloroplast RF19 (chloroplast) [Elaeagnus macrophylla])

HSP 1 Score: 263.5 bits (672), Expect = 3.5e-67
Identity = 146/180 (81.11%), Postives = 155/180 (86.11%), Query Frame = 1


Query: 30  WNNHKHLFDYGSTTRNSMRNLSIQCVFQNNLIFQLFNHFILPSSMLVRLVNISMFRCNNK 89
           WNNHKH FDYGST RNSMRNLSIQCVF NNLIFQLFNHFILPSSMLVRLVNI MFRCNNK
Sbjct: 104 WNNHKHFFDYGSTNRNSMRNLSIQCVFLNNLIFQLFNHFILPSSMLVRLVNIYMFRCNNK 163

Query: 90  MLFFC-----WLVNWSHFIQEMGWIDISLDQQNNSIRSNKYLVSELRNSMARIFSILLFI 149
           MLF       WL+    F++ +G + + + QQN+SIRSNKYLVSELRNSMARIFSILLFI
Sbjct: 164 MLFVTSSFVGWLIGHILFMKWVGLVLVWI-QQNHSIRSNKYLVSELRNSMARIFSILLFI 223

Query: 150 TCVYYLGRIPSPSVTKKLKETEKMEERGESEEETDVEIETTSETKGTKQEQEGSTEEDTS 205
           TCVYYLGRIPSP +TKKLKET K EERGESEEETDVEIETTSETK TKQEQEGSTEED S
Sbjct: 224 TCVYYLGRIPSPIITKKLKETSKTEERGESEEETDVEIETTSETKETKQEQEGSTEEDPS 282

[Close]

BLAST of Lsi07G010670.1 vs. NCBI nr
Match: gi|844170075|gb|AKM98071.1| (photosystem I assembly protein Ycf1 (chloroplast) [Anemone patens])

HSP 1 Score: 263.5 bits (672), Expect = 3.5e-67
Identity = 147/195 (75.38%), Postives = 159/195 (81.54%), Query Frame = 1


Query: 30  WNNHKHLFDYGSTTRNSMRNLSIQCVFQNNLIFQLFNHFILPSSMLVRLVNISMFRCNNK 89
           WNNHKH FDYGSTTRNSMRNLSIQCVF NNLIFQLFNHFILPSS L RLVNI MF+CNNK
Sbjct: 104 WNNHKHFFDYGSTTRNSMRNLSIQCVFLNNLIFQLFNHFILPSSTLARLVNIYMFQCNNK 163

Query: 90  MLFFC-----WLVNWSHFIQEMGWIDISLDQQNNSIRSNKYLVSELRNSMARIFSILLFI 149
           MLF       WL+    F++ +G +   + +QN+SIRSNKYLVSELRNSMARIFSILLFI
Sbjct: 164 MLFVTSSFVGWLIGHILFMKWVGLVLFWI-RQNHSIRSNKYLVSELRNSMARIFSILLFI 223

Query: 150 TCVYYLGRIPSPSVTKKLKETEKMEERGESEEETDVEIETTSETKGTKQEQEGSTEEDTS 209
           TCVYYLGRIPSP VTKKLKET K EERG+SEEE DVEIETTSETKGTKQEQEGS EED S
Sbjct: 224 TCVYYLGRIPSPIVTKKLKETSKKEERGKSEEEIDVEIETTSETKGTKQEQEGSAEEDPS 283

Query: 210 SEKRLCSYNTQIPSR 220
           S   LCS   + P +
Sbjct: 284 SSLSLCSEEKEDPDK 297

[Close]

BLAST of Lsi07G010670.1 vs. NCBI nr
Match: gi|938339493|ref|YP_009171646.1| (hypothetical chloroplast RF19 (chloroplast) [Elaeagnus macrophylla])

HSP 1 Score: 263.5 bits (672), Expect = 3.5e-67
Identity = 146/180 (81.11%), Postives = 155/180 (86.11%), Query Frame = 1


Query: 30  WNNHKHLFDYGSTTRNSMRNLSIQCVFQNNLIFQLFNHFILPSSMLVRLVNISMFRCNNK 89
           WNNHKH FDYGST RNSMRNLSIQCVF NNLIFQLFNHFILPSSMLVRLVNI MFRCNNK
Sbjct: 104 WNNHKHFFDYGSTNRNSMRNLSIQCVFLNNLIFQLFNHFILPSSMLVRLVNIYMFRCNNK 163

Query: 90  MLFFC-----WLVNWSHFIQEMGWIDISLDQQNNSIRSNKYLVSELRNSMARIFSILLFI 149
           MLF       WL+    F++ +G + + + QQN+SIRSNKYLVSELRNSMARIFSILLFI
Sbjct: 164 MLFVTSSFVGWLIGHILFMKWVGLVLVWI-QQNHSIRSNKYLVSELRNSMARIFSILLFI 223

Query: 150 TCVYYLGRIPSPSVTKKLKETEKMEERGESEEETDVEIETTSETKGTKQEQEGSTEEDTS 205
           TCVYYLGRIPSP +TKKLKET K EERGESEEETDVEIETTSETK TKQEQEGSTEED S
Sbjct: 224 TCVYYLGRIPSPIITKKLKETSKTEERGESEEETDVEIETTSETKETKQEQEGSTEEDPS 282

The following BLAST results are available for this feature:

BLAST of Lsi07G010670.1 vs. Swiss-Prot


Match Name	E-value	Identity	Description
TI214_DRIGR	6.0e-67	73.00	Protein TIC 214 OS=Drimys granadensis GN=TIC214 PE=3 SV=1	[more]
TI214_EUCGG	7.8e-67	77.66	Protein TIC 214 OS=Eucalyptus globulus subsp. globulus GN=TIC214 PE=3 SV=1	[more]
TI214_LIRTU	2.3e-66	73.00	Protein TIC 214 OS=Liriodendron tulipifera GN=TIC214 PE=3 SV=1	[more]
TI214_BUXMI	1.1e-65	72.50	Protein TIC 214 OS=Buxus microphylla GN=TIC214 PE=3 SV=1	[more]
T214A_PIPCE	1.5e-65	73.33	Putative protein TIC 214 N-terminal part OS=Piper cenocladum GN=TIC214 PE=5 SV=1	[more]

BLAST of Lsi07G010670.1 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0K1NPU1_9MYRT	1.4e-67	81.32	Hypothetical chloroplast RF19 OS=Eugenia uniflora GN=ycf1 PE=4 SV=1	[more]
A0A0A7LE95_9MAGN	1.9e-67	75.90	Hypothetical chloroplast RF1 OS=Clematis fusca var. coreana GN=ycf1 PE=4 SV=1	[more]
A0A0H3Y307_9MAGN	2.4e-67	75.38	Photosystem I assembly protein Ycf1 OS=Anemone patens GN=ycf1 PE=4 SV=1	[more]
A0A0H3XZS7_9MAGN	2.4e-67	75.38	Photosystem I assembly protein Ycf1 OS=Pulsatilla vernalis GN=ycf1 PE=4 SV=1	[more]
A0A0N7BUX5_9ROSA	2.4e-67	81.11	Hypothetical chloroplast RF19 OS=Elaeagnus macrophylla GN=ycf1 PE=4 SV=1	[more]

BLAST of Lsi07G010670.1 vs. TAIR10


Match Name	E-value	Identity	Description
ATCG01130.1	2.6e-52	65.41	ATCG01130.1 Ycf1 protein	[more]
ATCG01000.1	2.6e-52	65.41	ATCG01000.1 Ycf1 protein	[more]
AT2G07739.1	1.9e-26	87.30	Ycf1 protein	[more]
ATMG00370.1	1.9e-26	87.30	ATMG00370.1 Ycf1 protein	[more]

BLAST of Lsi07G010670.1 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|918021144\|ref\|YP_009163537.1\|	2.1e-67	81.32	hypothetical chloroplast RF19 (plastid) [Eugenia uniflora]	[more]
gi\|731443691\|gb\|AIZ57585.1\|	2.7e-67	75.90	hypothetical chloroplast RF1 (chloroplast) [Clematis fusca var. coreana]	[more]
gi\|938339504\|ref\|YP_009171657.1\|	3.5e-67	81.11	hypothetical chloroplast RF19 (chloroplast) [Elaeagnus macrophylla]	[more]
gi\|844170075\|gb\|AKM98071.1\|	3.5e-67	75.38	photosystem I assembly protein Ycf1 (chloroplast) [Anemone patens]	[more]
gi\|938339493\|ref\|YP_009171646.1\|	3.5e-67	81.11	hypothetical chloroplast RF19 (chloroplast) [Elaeagnus macrophylla]	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **Cellular Component**
Term	Definition
GO:0016021	integral component of membrane

Vocabulary: **INTERPRO**
Term	Definition
IPR008896	TIC214

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0009507	chloroplast
molecular_function	GO:0003674	molecular_function

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Lsi07G010670	Lsi07G010670	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lsi07G010670.1.CDS.3	Lsi07G010670.1.CDS.3	CDS
Lsi07G010670.1.CDS.2	Lsi07G010670.1.CDS.2	CDS
Lsi07G010670.1.CDS.1	Lsi07G010670.1.CDS.1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Lsi07G010670.1	Lsi07G010670.1-protein	polypeptide

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008896	Protein TIC214	PFAM	PF05758	Ycf1	coord: 30..205 score: 2.6
None	No IPR available	PANTHER	PTHR33163	FAMILY NOT NAMED	coord: 30..202 score: 9.5E