Lsi06G000040.1 (mRNA) Bottle gourd (USVL1VR-Ls)

NameLsi06G000040.1
TypemRNA
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls))
DescriptionEukaryotic peptide chain release factor subunit
Locationchr06 : 31286 .. 32379 (-)
Sequence length711
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCTTGGTGATGAATTTGGAACTACTTCGAACATTAAGAGCAGGGTAAATCGTCAATATGTTTTGGGCACTATCACATCTGCTCAGCAGAGGCTCAAGCTATATAACAAGGTTCCTCCAAATGGGCTTGTGTTGTATACTGGGACAATCGTGACTGAAGATGGAAAAGAAAAGAAAGTCAGAATTGATTTTGAGCCTTTCAGGCTATAAATGCATCTCTTTATCTCAACAACAACAAGTTCCACACGGAAGCCCTAAATGAACTGTTAGAATCTGATGACAAATTTGGCTTCATTGACATGGATGGTAATGGAACACTCTTTGGCACATTGAGTGGTAACACACGTGAAGTCCTGCACAAATTTAGTGTCAACCTTCCTAAGAAACATGGAAGAGGTGGTCAGTTAGCACTTCGGTTTGCTCGTCTTCGAATGGAGAAGCGACACAGTTATGTGAGGAAAACAACAAAGCTTGCAACCCAGTTCTTTATTAATCCTACCACTAGTCAACCTAATGTTGCAGGTTTGATACTGGTTGGATCAACTGACTTCAAAACGGAGCTCAGTCAATCCGACATGTTCGATCCTCGTCTCCAGGCCAAAATACTTAATGTTGTCGATGTCTCTTATGGAGGGGAAAATGGATTCAATCAGGCTATTCAATTGTCATCCGAGATCTTGTCTAATGTAAAATTTATATAGGAGAAGCGTCTAATTGGAAAATACTTTGAAGAGATTAGCCAGAATATAGGGAAATATGTTTTTGGTGTTGTTGAAATACTCATTGTTTGGGAAAATTTAGATATCAATAGGTACATAAAGAATGCTTCCACTGGTGAGATTGTTATAAAGCACTTGAACAAGGAATAGGAAGCCAATTAGAGCAACTTTCGAGAAAAGATGGCCTTACTGGAATGGTTTGCAAATGAGTACAAGAAGTTTGGTTGCACCCTAGAGTTCGTTACCAACAAATCACAGGAAGGATCACAGTTCTGCAAAGGTTTTGGTGGTATTGGAGGAATTCTTCGTTACCAGCTTGACATAAGATCGTTTGATGAACTATCTGATGGGGAAGAGTACGATGATTCTGAATAG

mRNA sequence

ATGCTTGGTGATGAATTTGGAACTACTTCGAACATTAAGAGCAGGGTAAATCGTCAATATGTTTTGGGCACTATCACATCTGCTCAGCAGAGGCTCAAGCTATATAACAAGGCTATAAATGCATCTCTTTATCTCAACAACAACAAGTTCCACACGGAAGCCCTAAATGAACTGTTAGAATCTGATGACAAATTTGGCTTCATTGACATGGATGGTAATGGAACACTCTTTGGCACATTGAGTGGTAACACACGTGAAGTCCTGCACAAATTTAGTGTCAACCTTCCTAAGAAACATGGAAGAGGTGGTCAGTTAGCACTTCGGTTTGCTCGTCTTCGAATGGAGAAGCGACACAGTTATGTGAGGAAAACAACAAAGCTTGCAACCCAGTTCTTTATTAATCCTACCACTAGTCAACCTAATGTTGCAGGTTTGATACTGGTTGGATCAACTGACTTCAAAACGGAGCTCAGTCAATCCGACATGTTCGATCCTCGTCTCCAGGCCAAAATACTTAATGTTGTCGATGTCTCTTATGGAGGGGAAAATGGATTCAATCAGGCTATTCAATTGTCATCCGAGATCTTGTCTAATGAAGGATCACAGTTCTGCAAAGGTTTTGGTGGTATTGGAGGAATTCTTCGTTACCAGCTTGACATAAGATCGTTTGATGAACTATCTGATGGGGAAGAGTACGATGATTCTGAATAG

Coding sequence (CDS)

ATGCTTGGTGATGAATTTGGAACTACTTCGAACATTAAGAGCAGGGTAAATCGTCAATATGTTTTGGGCACTATCACATCTGCTCAGCAGAGGCTCAAGCTATATAACAAGGCTATAAATGCATCTCTTTATCTCAACAACAACAAGTTCCACACGGAAGCCCTAAATGAACTGTTAGAATCTGATGACAAATTTGGCTTCATTGACATGGATGGTAATGGAACACTCTTTGGCACATTGAGTGGTAACACACGTGAAGTCCTGCACAAATTTAGTGTCAACCTTCCTAAGAAACATGGAAGAGGTGGTCAGTTAGCACTTCGGTTTGCTCGTCTTCGAATGGAGAAGCGACACAGTTATGTGAGGAAAACAACAAAGCTTGCAACCCAGTTCTTTATTAATCCTACCACTAGTCAACCTAATGTTGCAGGTTTGATACTGGTTGGATCAACTGACTTCAAAACGGAGCTCAGTCAATCCGACATGTTCGATCCTCGTCTCCAGGCCAAAATACTTAATGTTGTCGATGTCTCTTATGGAGGGGAAAATGGATTCAATCAGGCTATTCAATTGTCATCCGAGATCTTGTCTAATGAAGGATCACAGTTCTGCAAAGGTTTTGGTGGTATTGGAGGAATTCTTCGTTACCAGCTTGACATAAGATCGTTTGATGAACTATCTGATGGGGAAGAGTACGATGATTCTGAATAG

Protein sequence

MLGDEFGTTSNIKSRVNRQYVLGTITSAQQRLKLYNKAINASLYLNNNKFHTEALNELLESDDKFGFIDMDGNGTLFGTLSGNTREVLHKFSVNLPKKHGRGGQLALRFARLRMEKRHSYVRKTTKLATQFFINPTTSQPNVAGLILVGSTDFKTELSQSDMFDPRLQAKILNVVDVSYGGENGFNQAIQLSSEILSNEGSQFCKGFGGIGGILRYQLDIRSFDELSDGEEYDDSE
BLAST of Lsi06G000040.1 vs. Swiss-Prot
Match: ERF1X_ARATH (Eukaryotic peptide chain release factor subunit 1-1 OS=Arabidopsis thaliana GN=ERF1-1 PE=1 SV=2)

HSP 1 Score: 330.5 bits (846), Expect = 1.5e-89
Identity = 174/229 (75.98%), Postives = 186/229 (81.22%), Query Frame = 1

Query: 1   MLGDEFGTTSNIKSRVNRQYVLGTITSAQQRLKLYNKA---------------------- 60
           MLGDE+GT SNIKSRVNRQ VLG ITSAQQRLKLYN+                       
Sbjct: 50  MLGDEYGTASNIKSRVNRQSVLGAITSAQQRLKLYNRVPPNGLVLYTGTIVNEDGKEKKV 109

Query: 61  ---------INASLYLNNNKFHTEALNELLESDDKFGFIDMDGNGTLFGTLSGNTREVLH 120
                    INASLYL +NKFHTEALNELLESDDKFGFI MDGNGTLFGTLSGNTREVLH
Sbjct: 110 TIDFEPFRPINASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLH 169

Query: 121 KFSVNLPKKHGRGGQLALRFARLRMEKRHSYVRKTTKLATQFFINPTTSQPNVAGLILVG 180
           KFSV+LPKKHGRGGQ ALRFARLRMEKRH+YVRKT +LATQ++INP TSQPNV+GLIL G
Sbjct: 170 KFSVDLPKKHGRGGQSALRFARLRMEKRHNYVRKTAELATQYYINPATSQPNVSGLILAG 229

Query: 181 STDFKTELSQSDMFDPRLQAKILNVVDVSYGGENGFNQAIQLSSEILSN 199
           S DFKTELSQSDMFDPRL AKILNVVDVSYGGENGFNQAI+LS+EIL+N
Sbjct: 230 SADFKTELSQSDMFDPRLAAKILNVVDVSYGGENGFNQAIELSAEILAN 278

BLAST of Lsi06G000040.1 vs. Swiss-Prot
Match: ERF1Y_ARATH (Eukaryotic peptide chain release factor subunit 1-2 OS=Arabidopsis thaliana GN=ERF1-2 PE=1 SV=1)

HSP 1 Score: 330.1 bits (845), Expect = 2.0e-89
Identity = 174/229 (75.98%), Postives = 186/229 (81.22%), Query Frame = 1

Query: 1   MLGDEFGTTSNIKSRVNRQYVLGTITSAQQRLKLYNKA---------------------- 60
           MLGDE+GT SNIKSRVNRQ VL  ITSAQQRLKLYNK                       
Sbjct: 48  MLGDEYGTASNIKSRVNRQSVLSAITSAQQRLKLYNKVPTNGLVLYTGTIVNDDGKEKKV 107

Query: 61  ---------INASLYLNNNKFHTEALNELLESDDKFGFIDMDGNGTLFGTLSGNTREVLH 120
                    INASLYL +NKFHTEALNELLESDDKFGFI MDGNGTLFGTLSGNTREVLH
Sbjct: 108 TFDFEPFRPINASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLH 167

Query: 121 KFSVNLPKKHGRGGQLALRFARLRMEKRHSYVRKTTKLATQFFINPTTSQPNVAGLILVG 180
           KF+V+LPKKHGRGGQ ALRFARLRMEKRH+YVRKT +LATQF+INP TSQPNV+GLIL G
Sbjct: 168 KFTVDLPKKHGRGGQSALRFARLRMEKRHNYVRKTAELATQFYINPATSQPNVSGLILAG 227

Query: 181 STDFKTELSQSDMFDPRLQAKILNVVDVSYGGENGFNQAIQLSSEILSN 199
           S DFKTELSQS++FDPRLQAKILNVVDVSYGGENGFNQAI+LS+EILSN
Sbjct: 228 SADFKTELSQSELFDPRLQAKILNVVDVSYGGENGFNQAIELSAEILSN 276

BLAST of Lsi06G000040.1 vs. Swiss-Prot
Match: ERF1Z_ARATH (Eukaryotic peptide chain release factor subunit 1-3 OS=Arabidopsis thaliana GN=ERF1-3 PE=1 SV=1)

HSP 1 Score: 325.9 bits (834), Expect = 3.7e-88
Identity = 172/229 (75.11%), Postives = 184/229 (80.35%), Query Frame = 1

Query: 1   MLGDEFGTTSNIKSRVNRQYVLGTITSAQQRLKLYNKA---------------------- 60
           ML DE+GT SNIKSRVNRQ VL  ITSAQQRLKLYNK                       
Sbjct: 49  MLADEYGTASNIKSRVNRQSVLSAITSAQQRLKLYNKVPPNGLVLYTGTIVTDDGKEKKV 108

Query: 61  ---------INASLYLNNNKFHTEALNELLESDDKFGFIDMDGNGTLFGTLSGNTREVLH 120
                    INASLYL +NKFHTE LNELLESDDKFGFI MDGNGTLFGTLSGNTREVLH
Sbjct: 109 TIDFEPFKPINASLYLCDNKFHTEPLNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLH 168

Query: 121 KFSVNLPKKHGRGGQLALRFARLRMEKRHSYVRKTTKLATQFFINPTTSQPNVAGLILVG 180
           KF+V+LPKKHGRGGQ ALRFARLRMEKRH+YVRKT +LATQF+INP TSQPNV+GLIL G
Sbjct: 169 KFTVDLPKKHGRGGQSALRFARLRMEKRHNYVRKTAELATQFYINPATSQPNVSGLILAG 228

Query: 181 STDFKTELSQSDMFDPRLQAKILNVVDVSYGGENGFNQAIQLSSEILSN 199
           S DFKTELSQS++FDPRLQAKILNVVDVSYGGENGFNQAI+LS+EILSN
Sbjct: 229 SADFKTELSQSELFDPRLQAKILNVVDVSYGGENGFNQAIELSAEILSN 277

BLAST of Lsi06G000040.1 vs. Swiss-Prot
Match: ERF1_POLMI (Eukaryotic peptide chain release factor subunit 1 OS=Polyandrocarpa misakiensis GN=ERF1 PE=2 SV=1)

HSP 1 Score: 285.0 bits (728), Expect = 7.3e-76
Identity = 154/229 (67.25%), Postives = 166/229 (72.49%), Query Frame = 1

Query: 1   MLGDEFGTTSNIKSRVNRQYVLGTITSAQQRLKLYNKA---------------------- 60
           ML DEFGT SNIKSRVNR  VLG ITS QQRLKLYNK                       
Sbjct: 51  MLADEFGTASNIKSRVNRLSVLGAITSVQQRLKLYNKVPPNGLVVYCGTIVTDEGKEKKV 110

Query: 61  ---------INASLYLNNNKFHTEALNELLESDDKFGFIDMDGNGTLFGTLSGNTREVLH 120
                    IN SLYL +NKFHTEAL  LL  D+KFGFI MDGNG LFGTL GNTREVLH
Sbjct: 111 NIDFEPFKPINTSLYLCDNKFHTEALTALLSDDNKFGFIVMDGNGALFGTLQGNTREVLH 170

Query: 121 KFSVNLPKKHGRGGQLALRFARLRMEKRHSYVRKTTKLATQFFINPTTSQPNVAGLILVG 180
           KF+V+LPKKHGRGGQ ALRFARLRMEKRH+YVRK  + A Q FI  T  +PNV GL+L G
Sbjct: 171 KFTVDLPKKHGRGGQSALRFARLRMEKRHNYVRKVAETAVQLFI--TNDRPNVTGLVLAG 230

Query: 181 STDFKTELSQSDMFDPRLQAKILNVVDVSYGGENGFNQAIQLSSEILSN 199
           S DFKTELSQSDMFD RLQ KIL +VDVSYGGENGFNQAI+LS+E+LSN
Sbjct: 231 SADFKTELSQSDMFDQRLQVKILKLVDVSYGGENGFNQAIELSAEVLSN 277

BLAST of Lsi06G000040.1 vs. Swiss-Prot
Match: ERF1_DROME (Eukaryotic peptide chain release factor subunit 1 OS=Drosophila melanogaster GN=eRF1 PE=1 SV=2)

HSP 1 Score: 282.3 bits (721), Expect = 4.7e-75
Identity = 151/229 (65.94%), Postives = 167/229 (72.93%), Query Frame = 1

Query: 1   MLGDEFGTTSNIKSRVNRQYVLGTITSAQQRLKLYNKA---------------------- 60
           ML DEFGT SNIKSRVNR  VLG ITS Q RLKLY K                       
Sbjct: 51  MLADEFGTASNIKSRVNRLSVLGAITSVQHRLKLYTKVPPNGLVIYCGTIVTEEGKEKKV 110

Query: 61  ---------INASLYLNNNKFHTEALNELLESDDKFGFIDMDGNGTLFGTLSGNTREVLH 120
                    IN SLYL +NKFHTEAL  LL  D+KFGFI MDGNG LFGTL GNTREVLH
Sbjct: 111 NIDFEPFKPINTSLYLCDNKFHTEALTALLADDNKFGFIVMDGNGALFGTLQGNTREVLH 170

Query: 121 KFSVNLPKKHGRGGQLALRFARLRMEKRHSYVRKTTKLATQFFINPTTSQPNVAGLILVG 180
           KF+V+LPKKHGRGGQ ALRFARLRMEKRH+YVRK  ++ATQ FI  T  +PN+AGLIL G
Sbjct: 171 KFTVDLPKKHGRGGQSALRFARLRMEKRHNYVRKVAEVATQLFI--TNDKPNIAGLILAG 230

Query: 181 STDFKTELSQSDMFDPRLQAKILNVVDVSYGGENGFNQAIQLSSEILSN 199
           S DFKTELSQSDMFDPRLQ+K++ +VDVSYGGENGFNQAI+L++E L N
Sbjct: 231 SADFKTELSQSDMFDPRLQSKVIKLVDVSYGGENGFNQAIELAAESLQN 277

BLAST of Lsi06G000040.1 vs. TrEMBL
Match: E5GB47_CUCME (Eukaryotic peptide chain release factor subunit OS=Cucumis melo subsp. melo PE=4 SV=1)

HSP 1 Score: 340.9 bits (873), Expect = 1.2e-90
Identity = 182/229 (79.48%), Postives = 187/229 (81.66%), Query Frame = 1

Query: 1   MLGDEFGTTSNIKSRVNRQYVLGTITSAQQRLKLYNKA---------------------- 60
           MLGDEFGT SNIKSRVNRQ VLG ITSAQQRLKLYNK                       
Sbjct: 50  MLGDEFGTASNIKSRVNRQSVLGAITSAQQRLKLYNKVPPNGLVLYTGTIVTEDGKEKKV 109

Query: 61  ---------INASLYLNNNKFHTEALNELLESDDKFGFIDMDGNGTLFGTLSGNTREVLH 120
                    INASLYL +NKFHTEALNELLESDDKFGFI MDGNGTLFGTLSGNTREVLH
Sbjct: 110 TIDFEPFRPINASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLH 169

Query: 121 KFSVNLPKKHGRGGQLALRFARLRMEKRHSYVRKTTKLATQFFINPTTSQPNVAGLILVG 180
           KFSV+LPKKHGRGGQ ALRFARLRMEKRH+YVRKT +LATQFFINP TSQPNVAGLIL G
Sbjct: 170 KFSVDLPKKHGRGGQSALRFARLRMEKRHNYVRKTAELATQFFINPATSQPNVAGLILAG 229

Query: 181 STDFKTELSQSDMFDPRLQAKILNVVDVSYGGENGFNQAIQLSSEILSN 199
           S DFKTELSQSDMFDPRLQAKILNVVDVSYGGENGFNQAI+LSSEILSN
Sbjct: 230 SADFKTELSQSDMFDPRLQAKILNVVDVSYGGENGFNQAIELSSEILSN 278

BLAST of Lsi06G000040.1 vs. TrEMBL
Match: A0A0A0KF54_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G089230 PE=4 SV=1)

HSP 1 Score: 340.9 bits (873), Expect = 1.2e-90
Identity = 182/229 (79.48%), Postives = 187/229 (81.66%), Query Frame = 1

Query: 1   MLGDEFGTTSNIKSRVNRQYVLGTITSAQQRLKLYNKA---------------------- 60
           MLGDEFGT SNIKSRVNRQ VLG ITSAQQRLKLYNK                       
Sbjct: 50  MLGDEFGTASNIKSRVNRQSVLGAITSAQQRLKLYNKVPPNGLVLYTGTIVTEDGKEKKV 109

Query: 61  ---------INASLYLNNNKFHTEALNELLESDDKFGFIDMDGNGTLFGTLSGNTREVLH 120
                    INASLYL +NKFHTEALNELLESDDKFGFI MDGNGTLFGTLSGNTREVLH
Sbjct: 110 TIDFEPFRPINASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLH 169

Query: 121 KFSVNLPKKHGRGGQLALRFARLRMEKRHSYVRKTTKLATQFFINPTTSQPNVAGLILVG 180
           KFSV+LPKKHGRGGQ ALRFARLRMEKRH+YVRKT +LATQFFINP TSQPNVAGLIL G
Sbjct: 170 KFSVDLPKKHGRGGQSALRFARLRMEKRHNYVRKTAELATQFFINPATSQPNVAGLILAG 229

Query: 181 STDFKTELSQSDMFDPRLQAKILNVVDVSYGGENGFNQAIQLSSEILSN 199
           S DFKTELSQSDMFDPRLQAKILNVVDVSYGGENGFNQAI+LSSEILSN
Sbjct: 230 SADFKTELSQSDMFDPRLQAKILNVVDVSYGGENGFNQAIELSSEILSN 278

BLAST of Lsi06G000040.1 vs. TrEMBL
Match: A0A0A0KZH4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G293340 PE=4 SV=1)

HSP 1 Score: 340.9 bits (873), Expect = 1.2e-90
Identity = 182/229 (79.48%), Postives = 187/229 (81.66%), Query Frame = 1

Query: 1   MLGDEFGTTSNIKSRVNRQYVLGTITSAQQRLKLYNKA---------------------- 60
           MLGDEFGT SNIKSRVNRQ VLG ITSAQQRLKLYNK                       
Sbjct: 50  MLGDEFGTASNIKSRVNRQSVLGAITSAQQRLKLYNKVPPNGLVLYTGTIVTEDGKEKKV 109

Query: 61  ---------INASLYLNNNKFHTEALNELLESDDKFGFIDMDGNGTLFGTLSGNTREVLH 120
                    INASLYL +NKFHTEALNELLESDDKFGFI MDGNGTLFGTLSGNTREVLH
Sbjct: 110 TIDFEPFRPINASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLH 169

Query: 121 KFSVNLPKKHGRGGQLALRFARLRMEKRHSYVRKTTKLATQFFINPTTSQPNVAGLILVG 180
           KFSV+LPKKHGRGGQ ALRFARLRMEKRH+YVRKT +LATQFFINP TSQPNVAGLIL G
Sbjct: 170 KFSVDLPKKHGRGGQSALRFARLRMEKRHNYVRKTAELATQFFINPATSQPNVAGLILAG 229

Query: 181 STDFKTELSQSDMFDPRLQAKILNVVDVSYGGENGFNQAIQLSSEILSN 199
           S DFKTELSQSDMFDPRLQAKILNVVDVSYGGENGFNQAI+LSSEILSN
Sbjct: 230 SADFKTELSQSDMFDPRLQAKILNVVDVSYGGENGFNQAIELSSEILSN 278

BLAST of Lsi06G000040.1 vs. TrEMBL
Match: A0A072UM30_MEDTR (Eukaryotic peptide chain release factor subunit 1-3 OS=Medicago truncatula GN=MTR_6g478120 PE=4 SV=1)

HSP 1 Score: 339.7 bits (870), Expect = 2.8e-90
Identity = 181/229 (79.04%), Postives = 187/229 (81.66%), Query Frame = 1

Query: 1   MLGDEFGTTSNIKSRVNRQYVLGTITSAQQRLKLYNKA---------------------- 60
           MLGDEFGT SNIKSRVNRQ VLG ITSAQQRLKLYNK                       
Sbjct: 50  MLGDEFGTASNIKSRVNRQSVLGAITSAQQRLKLYNKVPPNGLVLYTGTIVTDDGKEKKV 109

Query: 61  ---------INASLYLNNNKFHTEALNELLESDDKFGFIDMDGNGTLFGTLSGNTREVLH 120
                    INASLYL +NKFHTEALNELLESDDKFGFI MDGNGTLFGTLSGNTREVLH
Sbjct: 110 TIDFEPFRPINASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLH 169

Query: 121 KFSVNLPKKHGRGGQLALRFARLRMEKRHSYVRKTTKLATQFFINPTTSQPNVAGLILVG 180
           KFSV+LPKKHGRGGQ ALRFARLRMEKRH+YVRKT +LATQF+INP TSQPNVAGLIL G
Sbjct: 170 KFSVDLPKKHGRGGQSALRFARLRMEKRHNYVRKTAELATQFYINPATSQPNVAGLILAG 229

Query: 181 STDFKTELSQSDMFDPRLQAKILNVVDVSYGGENGFNQAIQLSSEILSN 199
           S DFKTELSQSDMFDPRLQAKILNVVDVSYGGENGFNQAI+LSSEILSN
Sbjct: 230 SADFKTELSQSDMFDPRLQAKILNVVDVSYGGENGFNQAIELSSEILSN 278

BLAST of Lsi06G000040.1 vs. TrEMBL
Match: A0A067JTC7_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_23058 PE=4 SV=1)

HSP 1 Score: 339.7 bits (870), Expect = 2.8e-90
Identity = 181/229 (79.04%), Postives = 187/229 (81.66%), Query Frame = 1

Query: 1   MLGDEFGTTSNIKSRVNRQYVLGTITSAQQRLKLYNKA---------------------- 60
           MLGDEFGT SNIKSRVNRQ VLG ITSAQQRLKLYNK                       
Sbjct: 50  MLGDEFGTASNIKSRVNRQSVLGAITSAQQRLKLYNKVPSNGLVLYTGTIVTDDGKEKKV 109

Query: 61  ---------INASLYLNNNKFHTEALNELLESDDKFGFIDMDGNGTLFGTLSGNTREVLH 120
                    INASLYL +NKFHTEALNELLESDDKFGFI MDGNGTLFGTLSGNTREVLH
Sbjct: 110 TIDFEPFRPINASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLH 169

Query: 121 KFSVNLPKKHGRGGQLALRFARLRMEKRHSYVRKTTKLATQFFINPTTSQPNVAGLILVG 180
           KF+V+LPKKHGRGGQ ALRFARLRMEKRH+YVRKT +LATQFFINP TSQPNVAGLIL G
Sbjct: 170 KFTVDLPKKHGRGGQSALRFARLRMEKRHNYVRKTAELATQFFINPATSQPNVAGLILAG 229

Query: 181 STDFKTELSQSDMFDPRLQAKILNVVDVSYGGENGFNQAIQLSSEILSN 199
           S DFKTELSQSDMFDPRLQAKILNVVDVSYGGENGFNQAI+LSSEILSN
Sbjct: 230 SADFKTELSQSDMFDPRLQAKILNVVDVSYGGENGFNQAIELSSEILSN 278

BLAST of Lsi06G000040.1 vs. TAIR10
Match: AT5G47880.1 (AT5G47880.1 eukaryotic release factor 1-1)

HSP 1 Score: 330.5 bits (846), Expect = 8.6e-91
Identity = 174/229 (75.98%), Postives = 186/229 (81.22%), Query Frame = 1

Query: 1   MLGDEFGTTSNIKSRVNRQYVLGTITSAQQRLKLYNKA---------------------- 60
           MLGDE+GT SNIKSRVNRQ VLG ITSAQQRLKLYN+                       
Sbjct: 50  MLGDEYGTASNIKSRVNRQSVLGAITSAQQRLKLYNRVPPNGLVLYTGTIVNEDGKEKKV 109

Query: 61  ---------INASLYLNNNKFHTEALNELLESDDKFGFIDMDGNGTLFGTLSGNTREVLH 120
                    INASLYL +NKFHTEALNELLESDDKFGFI MDGNGTLFGTLSGNTREVLH
Sbjct: 110 TIDFEPFRPINASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLH 169

Query: 121 KFSVNLPKKHGRGGQLALRFARLRMEKRHSYVRKTTKLATQFFINPTTSQPNVAGLILVG 180
           KFSV+LPKKHGRGGQ ALRFARLRMEKRH+YVRKT +LATQ++INP TSQPNV+GLIL G
Sbjct: 170 KFSVDLPKKHGRGGQSALRFARLRMEKRHNYVRKTAELATQYYINPATSQPNVSGLILAG 229

Query: 181 STDFKTELSQSDMFDPRLQAKILNVVDVSYGGENGFNQAIQLSSEILSN 199
           S DFKTELSQSDMFDPRL AKILNVVDVSYGGENGFNQAI+LS+EIL+N
Sbjct: 230 SADFKTELSQSDMFDPRLAAKILNVVDVSYGGENGFNQAIELSAEILAN 278

BLAST of Lsi06G000040.1 vs. TAIR10
Match: AT1G12920.1 (AT1G12920.1 eukaryotic release factor 1-2)

HSP 1 Score: 330.1 bits (845), Expect = 1.1e-90
Identity = 174/229 (75.98%), Postives = 186/229 (81.22%), Query Frame = 1

Query: 1   MLGDEFGTTSNIKSRVNRQYVLGTITSAQQRLKLYNKA---------------------- 60
           MLGDE+GT SNIKSRVNRQ VL  ITSAQQRLKLYNK                       
Sbjct: 48  MLGDEYGTASNIKSRVNRQSVLSAITSAQQRLKLYNKVPTNGLVLYTGTIVNDDGKEKKV 107

Query: 61  ---------INASLYLNNNKFHTEALNELLESDDKFGFIDMDGNGTLFGTLSGNTREVLH 120
                    INASLYL +NKFHTEALNELLESDDKFGFI MDGNGTLFGTLSGNTREVLH
Sbjct: 108 TFDFEPFRPINASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLH 167

Query: 121 KFSVNLPKKHGRGGQLALRFARLRMEKRHSYVRKTTKLATQFFINPTTSQPNVAGLILVG 180
           KF+V+LPKKHGRGGQ ALRFARLRMEKRH+YVRKT +LATQF+INP TSQPNV+GLIL G
Sbjct: 168 KFTVDLPKKHGRGGQSALRFARLRMEKRHNYVRKTAELATQFYINPATSQPNVSGLILAG 227

Query: 181 STDFKTELSQSDMFDPRLQAKILNVVDVSYGGENGFNQAIQLSSEILSN 199
           S DFKTELSQS++FDPRLQAKILNVVDVSYGGENGFNQAI+LS+EILSN
Sbjct: 228 SADFKTELSQSELFDPRLQAKILNVVDVSYGGENGFNQAIELSAEILSN 276

BLAST of Lsi06G000040.1 vs. TAIR10
Match: AT3G26618.1 (AT3G26618.1 eukaryotic release factor 1-3)

HSP 1 Score: 325.9 bits (834), Expect = 2.1e-89
Identity = 172/229 (75.11%), Postives = 184/229 (80.35%), Query Frame = 1

Query: 1   MLGDEFGTTSNIKSRVNRQYVLGTITSAQQRLKLYNKA---------------------- 60
           ML DE+GT SNIKSRVNRQ VL  ITSAQQRLKLYNK                       
Sbjct: 49  MLADEYGTASNIKSRVNRQSVLSAITSAQQRLKLYNKVPPNGLVLYTGTIVTDDGKEKKV 108

Query: 61  ---------INASLYLNNNKFHTEALNELLESDDKFGFIDMDGNGTLFGTLSGNTREVLH 120
                    INASLYL +NKFHTE LNELLESDDKFGFI MDGNGTLFGTLSGNTREVLH
Sbjct: 109 TIDFEPFKPINASLYLCDNKFHTEPLNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLH 168

Query: 121 KFSVNLPKKHGRGGQLALRFARLRMEKRHSYVRKTTKLATQFFINPTTSQPNVAGLILVG 180
           KF+V+LPKKHGRGGQ ALRFARLRMEKRH+YVRKT +LATQF+INP TSQPNV+GLIL G
Sbjct: 169 KFTVDLPKKHGRGGQSALRFARLRMEKRHNYVRKTAELATQFYINPATSQPNVSGLILAG 228

Query: 181 STDFKTELSQSDMFDPRLQAKILNVVDVSYGGENGFNQAIQLSSEILSN 199
           S DFKTELSQS++FDPRLQAKILNVVDVSYGGENGFNQAI+LS+EILSN
Sbjct: 229 SADFKTELSQSELFDPRLQAKILNVVDVSYGGENGFNQAIELSAEILSN 277

BLAST of Lsi06G000040.1 vs. NCBI nr
Match: gi|449445606|ref|XP_004140563.1| (PREDICTED: eukaryotic peptide chain release factor subunit 1-3-like [Cucumis sativus])

HSP 1 Score: 340.9 bits (873), Expect = 1.8e-90
Identity = 182/229 (79.48%), Postives = 187/229 (81.66%), Query Frame = 1

Query: 1   MLGDEFGTTSNIKSRVNRQYVLGTITSAQQRLKLYNKA---------------------- 60
           MLGDEFGT SNIKSRVNRQ VLG ITSAQQRLKLYNK                       
Sbjct: 50  MLGDEFGTASNIKSRVNRQSVLGAITSAQQRLKLYNKVPPNGLVLYTGTIVTEDGKEKKV 109

Query: 61  ---------INASLYLNNNKFHTEALNELLESDDKFGFIDMDGNGTLFGTLSGNTREVLH 120
                    INASLYL +NKFHTEALNELLESDDKFGFI MDGNGTLFGTLSGNTREVLH
Sbjct: 110 TIDFEPFRPINASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLH 169

Query: 121 KFSVNLPKKHGRGGQLALRFARLRMEKRHSYVRKTTKLATQFFINPTTSQPNVAGLILVG 180
           KFSV+LPKKHGRGGQ ALRFARLRMEKRH+YVRKT +LATQFFINP TSQPNVAGLIL G
Sbjct: 170 KFSVDLPKKHGRGGQSALRFARLRMEKRHNYVRKTAELATQFFINPATSQPNVAGLILAG 229

Query: 181 STDFKTELSQSDMFDPRLQAKILNVVDVSYGGENGFNQAIQLSSEILSN 199
           S DFKTELSQSDMFDPRLQAKILNVVDVSYGGENGFNQAI+LSSEILSN
Sbjct: 230 SADFKTELSQSDMFDPRLQAKILNVVDVSYGGENGFNQAIELSSEILSN 278

BLAST of Lsi06G000040.1 vs. NCBI nr
Match: gi|659119950|ref|XP_008459930.1| (PREDICTED: eukaryotic peptide chain release factor subunit 1-3-like [Cucumis melo])

HSP 1 Score: 340.9 bits (873), Expect = 1.8e-90
Identity = 182/229 (79.48%), Postives = 187/229 (81.66%), Query Frame = 1

Query: 1   MLGDEFGTTSNIKSRVNRQYVLGTITSAQQRLKLYNKA---------------------- 60
           MLGDEFGT SNIKSRVNRQ VLG ITSAQQRLKLYNK                       
Sbjct: 50  MLGDEFGTASNIKSRVNRQSVLGAITSAQQRLKLYNKVPPNGLVLYTGTIVTEDGKEKKV 109

Query: 61  ---------INASLYLNNNKFHTEALNELLESDDKFGFIDMDGNGTLFGTLSGNTREVLH 120
                    INASLYL +NKFHTEALNELLESDDKFGFI MDGNGTLFGTLSGNTREVLH
Sbjct: 110 TIDFEPFRPINASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLH 169

Query: 121 KFSVNLPKKHGRGGQLALRFARLRMEKRHSYVRKTTKLATQFFINPTTSQPNVAGLILVG 180
           KFSV+LPKKHGRGGQ ALRFARLRMEKRH+YVRKT +LATQFFINP TSQPNVAGLIL G
Sbjct: 170 KFSVDLPKKHGRGGQSALRFARLRMEKRHNYVRKTAELATQFFINPATSQPNVAGLILAG 229

Query: 181 STDFKTELSQSDMFDPRLQAKILNVVDVSYGGENGFNQAIQLSSEILSN 199
           S DFKTELSQSDMFDPRLQAKILNVVDVSYGGENGFNQAI+LSSEILSN
Sbjct: 230 SADFKTELSQSDMFDPRLQAKILNVVDVSYGGENGFNQAIELSSEILSN 278

BLAST of Lsi06G000040.1 vs. NCBI nr
Match: gi|659068972|ref|XP_008447536.1| (PREDICTED: eukaryotic peptide chain release factor subunit 1-3 [Cucumis melo])

HSP 1 Score: 340.9 bits (873), Expect = 1.8e-90
Identity = 182/229 (79.48%), Postives = 187/229 (81.66%), Query Frame = 1

Query: 1   MLGDEFGTTSNIKSRVNRQYVLGTITSAQQRLKLYNKA---------------------- 60
           MLGDEFGT SNIKSRVNRQ VLG ITSAQQRLKLYNK                       
Sbjct: 50  MLGDEFGTASNIKSRVNRQSVLGAITSAQQRLKLYNKVPPNGLVLYTGTIVTEDGKEKKV 109

Query: 61  ---------INASLYLNNNKFHTEALNELLESDDKFGFIDMDGNGTLFGTLSGNTREVLH 120
                    INASLYL +NKFHTEALNELLESDDKFGFI MDGNGTLFGTLSGNTREVLH
Sbjct: 110 TIDFEPFRPINASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLH 169

Query: 121 KFSVNLPKKHGRGGQLALRFARLRMEKRHSYVRKTTKLATQFFINPTTSQPNVAGLILVG 180
           KFSV+LPKKHGRGGQ ALRFARLRMEKRH+YVRKT +LATQFFINP TSQPNVAGLIL G
Sbjct: 170 KFSVDLPKKHGRGGQSALRFARLRMEKRHNYVRKTAELATQFFINPATSQPNVAGLILAG 229

Query: 181 STDFKTELSQSDMFDPRLQAKILNVVDVSYGGENGFNQAIQLSSEILSN 199
           S DFKTELSQSDMFDPRLQAKILNVVDVSYGGENGFNQAI+LSSEILSN
Sbjct: 230 SADFKTELSQSDMFDPRLQAKILNVVDVSYGGENGFNQAIELSSEILSN 278

BLAST of Lsi06G000040.1 vs. NCBI nr
Match: gi|449448665|ref|XP_004142086.1| (PREDICTED: eukaryotic peptide chain release factor subunit 1-3 [Cucumis sativus])

HSP 1 Score: 340.9 bits (873), Expect = 1.8e-90
Identity = 182/229 (79.48%), Postives = 187/229 (81.66%), Query Frame = 1

Query: 1   MLGDEFGTTSNIKSRVNRQYVLGTITSAQQRLKLYNKA---------------------- 60
           MLGDEFGT SNIKSRVNRQ VLG ITSAQQRLKLYNK                       
Sbjct: 50  MLGDEFGTASNIKSRVNRQSVLGAITSAQQRLKLYNKVPPNGLVLYTGTIVTEDGKEKKV 109

Query: 61  ---------INASLYLNNNKFHTEALNELLESDDKFGFIDMDGNGTLFGTLSGNTREVLH 120
                    INASLYL +NKFHTEALNELLESDDKFGFI MDGNGTLFGTLSGNTREVLH
Sbjct: 110 TIDFEPFRPINASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLH 169

Query: 121 KFSVNLPKKHGRGGQLALRFARLRMEKRHSYVRKTTKLATQFFINPTTSQPNVAGLILVG 180
           KFSV+LPKKHGRGGQ ALRFARLRMEKRH+YVRKT +LATQFFINP TSQPNVAGLIL G
Sbjct: 170 KFSVDLPKKHGRGGQSALRFARLRMEKRHNYVRKTAELATQFFINPATSQPNVAGLILAG 229

Query: 181 STDFKTELSQSDMFDPRLQAKILNVVDVSYGGENGFNQAIQLSSEILSN 199
           S DFKTELSQSDMFDPRLQAKILNVVDVSYGGENGFNQAI+LSSEILSN
Sbjct: 230 SADFKTELSQSDMFDPRLQAKILNVVDVSYGGENGFNQAIELSSEILSN 278

BLAST of Lsi06G000040.1 vs. NCBI nr
Match: gi|225458331|ref|XP_002283027.1| (PREDICTED: eukaryotic peptide chain release factor subunit 1-3 [Vitis vinifera])

HSP 1 Score: 339.7 bits (870), Expect = 4.0e-90
Identity = 181/229 (79.04%), Postives = 187/229 (81.66%), Query Frame = 1

Query: 1   MLGDEFGTTSNIKSRVNRQYVLGTITSAQQRLKLYNKA---------------------- 60
           MLGDEFGT SNIKSRVNRQ VLG ITSAQQRLKLYNK                       
Sbjct: 50  MLGDEFGTASNIKSRVNRQSVLGAITSAQQRLKLYNKVPPNGLVLYTGTIVTEDGKEKKV 109

Query: 61  ---------INASLYLNNNKFHTEALNELLESDDKFGFIDMDGNGTLFGTLSGNTREVLH 120
                    INASLYL +NKFHTEALNELLESDDKFGFI MDGNGTLFGTLSGNTREVLH
Sbjct: 110 TIDFEPFRPINASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLH 169

Query: 121 KFSVNLPKKHGRGGQLALRFARLRMEKRHSYVRKTTKLATQFFINPTTSQPNVAGLILVG 180
           KF+V+LPKKHGRGGQ ALRFARLRMEKRH+YVRKT +LATQFFINP TSQPNVAGLIL G
Sbjct: 170 KFTVDLPKKHGRGGQSALRFARLRMEKRHNYVRKTAELATQFFINPATSQPNVAGLILAG 229

Query: 181 STDFKTELSQSDMFDPRLQAKILNVVDVSYGGENGFNQAIQLSSEILSN 199
           S DFKTELSQSDMFDPRLQAKILNVVDVSYGGENGFNQAI+LSSEILSN
Sbjct: 230 SADFKTELSQSDMFDPRLQAKILNVVDVSYGGENGFNQAIELSSEILSN 278

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
ERF1X_ARATH1.5e-8975.98Eukaryotic peptide chain release factor subunit 1-1 OS=Arabidopsis thaliana GN=E... [more]
ERF1Y_ARATH2.0e-8975.98Eukaryotic peptide chain release factor subunit 1-2 OS=Arabidopsis thaliana GN=E... [more]
ERF1Z_ARATH3.7e-8875.11Eukaryotic peptide chain release factor subunit 1-3 OS=Arabidopsis thaliana GN=E... [more]
ERF1_POLMI7.3e-7667.25Eukaryotic peptide chain release factor subunit 1 OS=Polyandrocarpa misakiensis ... [more]
ERF1_DROME4.7e-7565.94Eukaryotic peptide chain release factor subunit 1 OS=Drosophila melanogaster GN=... [more]
Match NameE-valueIdentityDescription
E5GB47_CUCME1.2e-9079.48Eukaryotic peptide chain release factor subunit OS=Cucumis melo subsp. melo PE=4... [more]
A0A0A0KF54_CUCSA1.2e-9079.48Uncharacterized protein OS=Cucumis sativus GN=Csa_6G089230 PE=4 SV=1[more]
A0A0A0KZH4_CUCSA1.2e-9079.48Uncharacterized protein OS=Cucumis sativus GN=Csa_4G293340 PE=4 SV=1[more]
A0A072UM30_MEDTR2.8e-9079.04Eukaryotic peptide chain release factor subunit 1-3 OS=Medicago truncatula GN=MT... [more]
A0A067JTC7_JATCU2.8e-9079.04Uncharacterized protein OS=Jatropha curcas GN=JCGZ_23058 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G47880.18.6e-9175.98 eukaryotic release factor 1-1[more]
AT1G12920.11.1e-9075.98 eukaryotic release factor 1-2[more]
AT3G26618.12.1e-8975.11 eukaryotic release factor 1-3[more]
Match NameE-valueIdentityDescription
gi|449445606|ref|XP_004140563.1|1.8e-9079.48PREDICTED: eukaryotic peptide chain release factor subunit 1-3-like [Cucumis sat... [more]
gi|659119950|ref|XP_008459930.1|1.8e-9079.48PREDICTED: eukaryotic peptide chain release factor subunit 1-3-like [Cucumis mel... [more]
gi|659068972|ref|XP_008447536.1|1.8e-9079.48PREDICTED: eukaryotic peptide chain release factor subunit 1-3 [Cucumis melo][more]
gi|449448665|ref|XP_004142086.1|1.8e-9079.48PREDICTED: eukaryotic peptide chain release factor subunit 1-3 [Cucumis sativus][more]
gi|225458331|ref|XP_002283027.1|4.0e-9079.04PREDICTED: eukaryotic peptide chain release factor subunit 1-3 [Vitis vinifera][more]
The following terms have been associated with this mRNA:
Vocabulary: Biological Process
TermDefinition
GO:0006415translational termination
Vocabulary: Cellular Component
TermDefinition
GO:0005737cytoplasm
Vocabulary: Molecular Function
TermDefinition
GO:0016149translation release factor activity, codon specific
Vocabulary: INTERPRO
TermDefinition
IPR024049Release_factor_eRF1/aRF1_N
IPR005142eRF1_3
IPR005141eRF1_2
IPR004403Peptide_chain-rel_eRF1/aRF1
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006415 translational termination
biological_process GO:0051083 'de novo' cotranslational protein folding
biological_process GO:0006449 regulation of translational termination
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005840 ribosome
cellular_component GO:0018444 translation release factor complex
molecular_function GO:0016149 translation release factor activity, codon specific

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Lsi06G000040Lsi06G000040gene


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Lsi06G000040.1.CDS.3Lsi06G000040.1.CDS.3CDS
Lsi06G000040.1.CDS.2Lsi06G000040.1.CDS.2CDS
Lsi06G000040.1.CDS.1Lsi06G000040.1.CDS.1CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Lsi06G000040.1Lsi06G000040.1-proteinpolypeptide


Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004403Peptide chain release factor eRF1/aRF1PANTHERPTHR10113PEPTIDE CHAIN RELEASE FACTOR SUBUNIT 1coord: 1..198
score: 1.0E
IPR005141eRF1 domain 2PFAMPF03464eRF1_2coord: 64..197
score: 4.5
IPR005142eRF1 domain 3PFAMPF03465eRF1_3coord: 197..218
score: 3.
IPR024049Peptide Chain Release Factor eRF1/aRF1, N-terminalGENE3DG3DSA:3.30.960.10coord: 1..44
score: 1.8
IPR024049Peptide Chain Release Factor eRF1/aRF1, N-terminalunknownSSF55481N-terminal domain of eukaryotic peptide chain release factor subunit 1, ERF1coord: 1..60
score: 8.11
NoneNo IPR availableGENE3DG3DSA:3.30.420.60coord: 62..197
score: 1.9
NoneNo IPR availablePANTHERPTHR10113:SF8EUKARYOTIC PEPTIDE CHAIN RELEASE FACTOR SUBUNIT 1-1-RELATEDcoord: 1..198
score: 1.0E
NoneNo IPR availableunknownSSF53137Translational machinery componentscoord: 62..196
score: 9.59