Lsi05G017020.1 (mRNA) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi05G017020.1
Type	mRNA
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Enoyl-CoA hydratase/isomerase family protein
Location	chr05 : 24564668 .. 24565387 (+)
Sequence length	720

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTGCACACTCGAGAAGCGACGCAATCTTTTCATCCTAACTCTGACCGGCGACGACGACCACCGCCTCAGCCCCTCCTTCATCGCCTCCATCCTCGAGGCTCTTTCCAAAGTCAAATCCCAAGCCTCCCGTGGCTCCGTTCTCATCACTACTTCCCATGGCAAATTCTTCTCTAACGGCTTCGATCTCTCCTGGGCTCAAGCCGCCGGCTCTGTTTCAGCCGCCGCCGAACGCCTCAGGCATATGGTCGAGATTTTCAAGCCCGTCGTCGCCCAACTCCTCTCCCTCCCCATGCCCACCATCGCTGCCATTGCAGGCCACGCCGCCGCGGCCGGCTTCATGTTAGCCCTCAGTCACGACTATTTGTTCATGAGGAGCGATCGAGGCGTTCTGTACATGAGCGAAGTGGATCTAGGGCTAAGTCTGCCCGATTACTTCACGGCGCTGTTTAAATCGAAGATCGGCTCGAGTTCCGTCCGCCGCGATGTGCTCTTGAGGGGAATGAAAGTGAAAGGTGAAGCGGCGGTGAGGATGGGGATCGTGGAATCGGCGCATGACGGAGAGGACGGTGTCATGAAGGCTGCCGTACGACTCGGGGAGCAGTTGGGAGCGAGAAATTGGAACGGCGAACCCTACGCAGAAATTAGGAAAAGTCTTCATCCAGAGATCTCTGGGCTGCTTGGACTGCCGGAAAAGGTCATATCGATTTCCAAGCTATGA

mRNA sequence

ATGTGCACACTCGAGAAGCGACGCAATCTTTTCATCCTAACTCTGACCGGCGACGACGACCACCGCCTCAGCCCCTCCTTCATCGCCTCCATCCTCGAGGCTCTTTCCAAAGTCAAATCCCAAGCCTCCCGTGGCTCCGTTCTCATCACTACTTCCCATGGCAAATTCTTCTCTAACGGCTTCGATCTCTCCTGGGCTCAAGCCGCCGGCTCTGTTTCAGCCGCCGCCGAACGCCTCAGGCATATGGTCGAGATTTTCAAGCCCGTCGTCGCCCAACTCCTCTCCCTCCCCATGCCCACCATCGCTGCCATTGCAGGCCACGCCGCCGCGGCCGGCTTCATGTTAGCCCTCAGTCACGACTATTTGTTCATGAGGAGCGATCGAGGCGTTCTGTACATGAGCGAAGTGGATCTAGGGCTAAGTCTGCCCGATTACTTCACGGCGCTGTTTAAATCGAAGATCGGCTCGAGTTCCGTCCGCCGCGATGTGCTCTTGAGGGGAATGAAAGTGAAAGGTGAAGCGGCGGTGAGGATGGGGATCGTGGAATCGGCGCATGACGGAGAGGACGGTGTCATGAAGGCTGCCGTACGACTCGGGGAGCAGTTGGGAGCGAGAAATTGGAACGGCGAACCCTACGCAGAAATTAGGAAAAGTCTTCATCCAGAGATCTCTGGGCTGCTTGGACTGCCGGAAAAGGTCATATCGATTTCCAAGCTATGA

Coding sequence (CDS)

ATGTGCACACTCGAGAAGCGACGCAATCTTTTCATCCTAACTCTGACCGGCGACGACGACCACCGCCTCAGCCCCTCCTTCATCGCCTCCATCCTCGAGGCTCTTTCCAAAGTCAAATCCCAAGCCTCCCGTGGCTCCGTTCTCATCACTACTTCCCATGGCAAATTCTTCTCTAACGGCTTCGATCTCTCCTGGGCTCAAGCCGCCGGCTCTGTTTCAGCCGCCGCCGAACGCCTCAGGCATATGGTCGAGATTTTCAAGCCCGTCGTCGCCCAACTCCTCTCCCTCCCCATGCCCACCATCGCTGCCATTGCAGGCCACGCCGCCGCGGCCGGCTTCATGTTAGCCCTCAGTCACGACTATTTGTTCATGAGGAGCGATCGAGGCGTTCTGTACATGAGCGAAGTGGATCTAGGGCTAAGTCTGCCCGATTACTTCACGGCGCTGTTTAAATCGAAGATCGGCTCGAGTTCCGTCCGCCGCGATGTGCTCTTGAGGGGAATGAAAGTGAAAGGTGAAGCGGCGGTGAGGATGGGGATCGTGGAATCGGCGCATGACGGAGAGGACGGTGTCATGAAGGCTGCCGTACGACTCGGGGAGCAGTTGGGAGCGAGAAATTGGAACGGCGAACCCTACGCAGAAATTAGGAAAAGTCTTCATCCAGAGATCTCTGGGCTGCTTGGACTGCCGGAAAAGGTCATATCGATTTCCAAGCTATGA

Protein sequence

MCTLEKRRNLFILTLTGDDDHRLSPSFIASILEALSKVKSQASRGSVLITTSHGKFFSNGFDLSWAQAAGSVSAAAERLRHMVEIFKPVVAQLLSLPMPTIAAIAGHAAAAGFMLALSHDYLFMRSDRGVLYMSEVDLGLSLPDYFTALFKSKIGSSSVRRDVLLRGMKVKGEAAVRMGIVESAHDGEDGVMKAAVRLGEQLGARNWNGEPYAEIRKSLHPEISGLLGLPEKVISISKL

Homology

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BLAST of Lsi05G017020.1 vs. Swiss-Prot
Match: ECI2_ARATH (Enoyl-CoA delta isomerase 2, peroxisomal OS=Arabidopsis thaliana GN=ECI2 PE=1 SV=1)

HSP 1 Score: 317.4 bits (812), Expect = 1.3e-85
Identity = 159/240 (66.25%), Postives = 199/240 (82.92%), Query Frame = 1


Query: 1   MCTLEKRRNLFILTLTGDDDHRLSPSFIASILEALSKVKSQASRGSVLITTSHGKFFSNG 60
           MCTLEKR +LF+LTLTGD +HR  P  IA+IL  L + KSQ++RGS+LITT++GKFFSNG
Sbjct: 1   MCTLEKRGDLFLLTLTGDGEHRFHPDTIATILSLLEQAKSQSTRGSILITTANGKFFSNG 60

Query: 61  FDLSWAQAAGSVSAAAERLRHMVEIFKPVVAQLLSLPMPTIAAIAGHAAAAGFMLALSHD 120
           FDL+WAQ AGS + AA RL  MVE FKPVVA LL LPMPTIAA+ GHAAAAG +LALSHD
Sbjct: 61  FDLAWAQTAGSKTGAANRLHQMVESFKPVVAALLDLPMPTIAALNGHAAAAGLILALSHD 120

Query: 121 YLFMRSDRGVLYMSEVDLGLSLPDYFTALFKSKIGSSSVRRDVLLRGMKVKGEAAVRMGI 180
           Y+FMR DRGVLYMSEVD+GLS+PDYF+AL ++KIG+S+ RR++LL G K++GE AV +GI
Sbjct: 121 YVFMRKDRGVLYMSEVDIGLSMPDYFSALVRAKIGTSAARRELLLSGKKIRGEEAVGLGI 180

Query: 181 VES-AHDGEDGVMKAAVRLGEQLGARNWNGEPYAEIRKSLHPEISGLLGLPEKVISISKL 240
           V+S A+D E+GV+ A+VRLGE+L A+ W+GE YA IRKSL+PE+ G+LGL  +V +  KL
Sbjct: 181 VDSAAYDSEEGVVVASVRLGEKLAAKKWSGEVYASIRKSLYPELCGILGLETRVFATPKL 240

[Close]

BLAST of Lsi05G017020.1 vs. Swiss-Prot
Match: ECI3_ARATH (Enoyl-CoA delta isomerase 3 OS=Arabidopsis thaliana GN=ECI3 PE=1 SV=1)

HSP 1 Score: 292.0 bits (746), Expect = 6.1e-78
Identity = 147/230 (63.91%), Postives = 183/230 (79.57%), Query Frame = 1


Query: 1   MCTLEKRRNLFILTLTGDDDHRLSPSFIASILEALSKVKSQASRGSVLITTSHGKFFSNG 60
           MCTLEKR +LF+LTLTG+D+HR  P  IAS+L  L + KSQ+++GSVLITT HGKFFSNG
Sbjct: 1   MCTLEKRGDLFLLTLTGEDEHRFHPDTIASVLSLLEQAKSQSTKGSVLITTGHGKFFSNG 60

Query: 61  FDLSWAQAAGSVSAAAERLRHMVEIFKPVVAQLLSLPMPTIAAIAGHAAAAGFMLALSHD 120
           FDL+WAQ+AG    A +R+  MV+ FKPV+A LL LPMPTIAA+ GHAAA+G M ALSHD
Sbjct: 61  FDLAWAQSAGH--GAIKRMHQMVKSFKPVLAALLDLPMPTIAALNGHAAASGLMFALSHD 120

Query: 121 YLFMRSDRGVLYMSEVDLGLSLPDYFTALFKSKIGSSSVRRDVLLRGMKVKGEAAVRMGI 180
           Y+FMR DRGVLYMSEVD+GL +PDYF+AL  +K+GS   RR++LL G K+KGE AV +GI
Sbjct: 121 YVFMRKDRGVLYMSEVDIGLPVPDYFSALVVAKVGSGIARRELLLSGKKLKGEEAVALGI 180

Query: 181 VES-AHDGEDGVMKAAVRLGEQLGARNWNGEPYAEIRKSLHPEISGLLGL 230
           V+S AHD  +GV++A V LGE L A+ WNGE YA IRKSL+PE+  ++ L
Sbjct: 181 VDSAAHDSAEGVVEATVSLGESLAAKKWNGEVYATIRKSLYPELCRMVDL 228

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BLAST of Lsi05G017020.1 vs. Swiss-Prot
Match: ECI1_ARATH (Enoyl-CoA delta isomerase 1, peroxisomal OS=Arabidopsis thaliana GN=ECI1 PE=1 SV=1)

HSP 1 Score: 206.1 bits (523), Expect = 4.4e-52
Identity = 109/224 (48.66%), Postives = 157/224 (70.09%), Query Frame = 1


Query: 1   MCTLEKRRNLFILTLTGDDDHRLSPSFIASILEALSKVKSQASRG-SVLITTSHGKFFSN 60
           MC+LEKR  LFIL LTGD +HRL+P+ + S+   +++++S  S   SVLITTS GKFFSN
Sbjct: 1   MCSLEKRDRLFILKLTGDGEHRLNPTLLDSLRSTINQIRSDPSFSQSVLITTSDGKFFSN 60

Query: 61  GFDLSWAQAAGSVSAAAERLRHMVEIFKPVVAQLLSLPMPTIAAIAGHAAAAGFMLALSH 120
           G+DL+ A++  S+S   +         + +VA L+SLPMPTIAA+ GHA+AAG +LA+SH
Sbjct: 61  GYDLALAESNPSLSVVMDAK------LRSLVADLISLPMPTIAAVTGHASAAGCILAMSH 120

Query: 121 DYLFMRSDRGVLYMSEVDLGLSLPDYFTALFKSKIGSSSVRRDVLLRGMKVKGEAAVRMG 180
           DY+ MR DRG LYMSE+D+ L +P +F A+ + KIGS + RRDV+L   KV  +  V+MG
Sbjct: 121 DYVLMRRDRGFLYMSELDIELIVPAWFMAVIRGKIGSPAARRDVMLTAAKVTADVGVKMG 180

Query: 181 IVESAHDGEDGVMKAAVRLGEQLGARNWNGEPYAEIRKSLHPEI 224
           IV+SA+      ++AA++LGE++  R  +G  Y ++R+SL  E+
Sbjct: 181 IVDSAYGSAAETVEAAIKLGEEIVQRGGDGHVYGKMRESLLREV 218

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BLAST of Lsi05G017020.1 vs. TrEMBL
Match: A0A0A0L8C7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G141780 PE=4 SV=1)

HSP 1 Score: 416.4 bits (1069), Expect = 2.4e-113
Identity = 215/239 (89.96%), Postives = 226/239 (94.56%), Query Frame = 1


Query: 1   MCTLEKRRNLFILTLTGDDDHRLSPSFIASILEALSKVKSQASRGSVLITTSHGKFFSNG 60
           MCTLEKRRNLFILTLTG DDHR SPS IA+ILEALSKVKSQAS GSVLITTSHGKFFSNG
Sbjct: 1   MCTLEKRRNLFILTLTGHDDHRFSPSVIAAILEALSKVKSQASTGSVLITTSHGKFFSNG 60

Query: 61  FDLSWAQAAGSVSAAAERLRHMVEIFKPVVAQLLSLPMPTIAAIAGHAAAAGFMLALSHD 120
           FDLSWAQAAGSVSAAAERL HMV+IFKPVVA+LLSLPMPTIAAI+GHAAAAGF+LALSHD
Sbjct: 61  FDLSWAQAAGSVSAAAERLHHMVQIFKPVVAELLSLPMPTIAAISGHAAAAGFLLALSHD 120

Query: 121 YLFMRSDRGVLYMSEVDLGLSLPDYFTALFKSKIGSSSVRRDVLLRGMKVKGEAAVRMGI 180
           YL MRSDRGVLYMSEVDLGLSLPDYF ALFKSKI SSSVRRDVL+RGMKVKGEAAV+MGI
Sbjct: 121 YLLMRSDRGVLYMSEVDLGLSLPDYFAALFKSKIASSSVRRDVLMRGMKVKGEAAVKMGI 180

Query: 181 VESAHDGEDGVMKAAVRLGEQLGARNWNGEPYAEIRKSLHPEISGLLGLPEKVISISKL 240
            +SAHDGE+GVM+AAVRLGEQLG R WNGEPYAEIRKSL+PEIS LLGLPEK ISISKL
Sbjct: 181 ADSAHDGENGVMEAAVRLGEQLGERKWNGEPYAEIRKSLYPEISRLLGLPEKAISISKL 239

[Close]

BLAST of Lsi05G017020.1 vs. TrEMBL
Match: A0A0A0L9X4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G141770 PE=4 SV=1)

HSP 1 Score: 371.7 bits (953), Expect = 6.7e-100
Identity = 191/239 (79.92%), Postives = 211/239 (88.28%), Query Frame = 1


Query: 1   MCTLEKRRNLFILTLTGDDDHRLSPSFIASILEALSKVKSQASRGSVLITTSHGKFFSNG 60
           MCT+EKR N FILTLTGDDDHRLSPS I SIL+ALS+VK+QAS  SVLITTSHGKFFSNG
Sbjct: 1   MCTVEKRGNFFILTLTGDDDHRLSPSLINSILKALSQVKAQASPASVLITTSHGKFFSNG 60

Query: 61  FDLSWAQAAGSVSAAAERLRHMVEIFKPVVAQLLSLPMPTIAAIAGHAAAAGFMLALSHD 120
           FDL WA +AGSVSAA  RL H++EIFK VVA+L+SLPMPTIAAI GHAAAAGF LALSHD
Sbjct: 61  FDLPWALSAGSVSAARHRLNHLIEIFKSVVAELISLPMPTIAAITGHAAAAGFTLALSHD 120

Query: 121 YLFMRSDRGVLYMSEVDLGLSLPDYFTALFKSKIGSSSVRRDVLLRGMKVKGEAAVRMGI 180
           YL MRSDRGVLYM E+DLGL+LPDYF ALFKSKIGSS   RD++LRGMKV+GEAAVRMGI
Sbjct: 121 YLIMRSDRGVLYMRELDLGLTLPDYFMALFKSKIGSSLAMRDLILRGMKVRGEAAVRMGI 180

Query: 181 VESAHDGEDGVMKAAVRLGEQLGARNWNGEPYAEIRKSLHPEISGLLGLPEKVISISKL 240
           V+SAHDGED V+ AAVRLGEQL  RNW+GE YAEIRKSL+PE+S +LGLP K ISISKL
Sbjct: 181 VDSAHDGEDSVVNAAVRLGEQLAGRNWDGEVYAEIRKSLYPEVSEVLGLPGKPISISKL 239

[Close]

BLAST of Lsi05G017020.1 vs. TrEMBL
Match: M5XMU9_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa010715mg PE=4 SV=1)

HSP 1 Score: 329.3 bits (843), Expect = 3.8e-87
Identity = 166/229 (72.49%), Postives = 192/229 (83.84%), Query Frame = 1


Query: 1   MCTLEKRRNLFILTLTGDDDHRLSPSFIASILEALSKVKSQASRGSVLITTSHGKFFSNG 60
           MCTLEKR +LF LTLT DD+HRLS + I S+L ALS+ KSQA+RGSVL+TT+HGKFFSNG
Sbjct: 1   MCTLEKRGDLFFLTLTCDDEHRLSLTLIDSLLSALSQAKSQATRGSVLVTTAHGKFFSNG 60

Query: 61  FDLSWAQAAGSVSAAAERLRHMVEIFKPVVAQLLSLPMPTIAAIAGHAAAAGFMLALSHD 120
           FDL WAQAAGS SAA  RL  MV  FKPVVA LLSLPMPT+AA+ GHAAAAGF+LALSHD
Sbjct: 61  FDLGWAQAAGSASAARARLSQMVAAFKPVVAALLSLPMPTVAAVQGHAAAAGFLLALSHD 120

Query: 121 YLFMRSDRGVLYMSEVDLGLSLPDYFTALFKSKIGSSSVRRDVLLRGMKVKGEAAVRMGI 180
           Y  MR DRGVLYMSEVDLGL  PDYFTA F +KIGS S RRDV+LRGMK+KG+ AV++GI
Sbjct: 121 YFLMRRDRGVLYMSEVDLGLPFPDYFTAAFLAKIGSVSGRRDVMLRGMKLKGDEAVKLGI 180

Query: 181 VESAHDGEDGVMKAAVRLGEQLGARNWNGEPYAEIRKSLHPEISGLLGL 230
           VESAHD  +  ++AAVRLGEQL  R WNG+ YAEIRKSL+PE+ G++G+
Sbjct: 181 VESAHDSAESTVEAAVRLGEQLAKRKWNGDVYAEIRKSLYPELCGVVGV 229

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BLAST of Lsi05G017020.1 vs. TrEMBL
Match: V4MKX3_EUTSA (Uncharacterized protein OS=Eutrema salsugineum GN=EUTSA_v10027007mg PE=4 SV=1)

HSP 1 Score: 327.8 bits (839), Expect = 1.1e-86
Identity = 163/240 (67.92%), Postives = 201/240 (83.75%), Query Frame = 1


Query: 1   MCTLEKRRNLFILTLTGDDDHRLSPSFIASILEALSKVKSQASRGSVLITTSHGKFFSNG 60
           MCTLEKR +LF+LTLTGD +HR  P+ IASI+  L + KSQ+ RGSVLITT++GKFFSNG
Sbjct: 48  MCTLEKRGDLFLLTLTGDGEHRFHPNTIASIISLLEQAKSQSKRGSVLITTANGKFFSNG 107

Query: 61  FDLSWAQAAGSVSAAAERLRHMVEIFKPVVAQLLSLPMPTIAAIAGHAAAAGFMLALSHD 120
           FDL+WAQ++GS +AAAERL HMVE FKPVVA LL LPMPTIAA+ GHAAAAG MLALSHD
Sbjct: 108 FDLAWAQSSGSKTAAAERLHHMVESFKPVVAALLDLPMPTIAALNGHAAAAGLMLALSHD 167

Query: 121 YLFMRSDRGVLYMSEVDLGLSLPDYFTALFKSKIGSSSVRRDVLLRGMKVKGEAAVRMGI 180
           Y+FMR+DRGVLYMSEVD+GLS+PDYF A+ +SKIG+S+ RR+V L G K++GE AV +GI
Sbjct: 168 YVFMRNDRGVLYMSEVDIGLSMPDYFAAIVRSKIGTSAARREVFLSGKKIRGEEAVALGI 227

Query: 181 VES-AHDGEDGVMKAAVRLGEQLGARNWNGEPYAEIRKSLHPEISGLLGLPEKVISISKL 240
           V+S AHD E+GV++A VRLGE+L A+ W+GE YA IRKSL+PE+ G+L L  K+ +  KL
Sbjct: 228 VDSAAHDSEEGVLEATVRLGEKLAAKKWSGEVYASIRKSLYPELCGMLNLEAKIFATPKL 287

[Close]

BLAST of Lsi05G017020.1 vs. TrEMBL
Match: A0A0D3DLS0_BRAOL (Uncharacterized protein OS=Brassica oleracea var. oleracea GN=IBR10 PE=4 SV=1)

HSP 1 Score: 323.6 bits (828), Expect = 2.1e-85
Identity = 162/240 (67.50%), Postives = 196/240 (81.67%), Query Frame = 1


Query: 1   MCTLEKRRNLFILTLTGDDDHRLSPSFIASILEALSKVKSQASRGSVLITTSHGKFFSNG 60
           MCTLEKR NLF+LTLTGD++HR +P  IA+IL  L + KSQA RGSVLITT HGKFFSNG
Sbjct: 1   MCTLEKRGNLFLLTLTGDNEHRFNPDAIATILSLLGQAKSQAKRGSVLITTGHGKFFSNG 60

Query: 61  FDLSWAQAAGSVSAAAERLRHMVEIFKPVVAQLLSLPMPTIAAIAGHAAAAGFMLALSHD 120
           FDL+WAQA+GS++ AAERL  MVE FKPVVA LL LPMPTIAA+ GHAAAAG MLALSHD
Sbjct: 61  FDLAWAQASGSLTGAAERLHQMVESFKPVVAALLDLPMPTIAALNGHAAAAGMMLALSHD 120

Query: 121 YLFMRSDRGVLYMSEVDLGLSLPDYFTALFKSKIGSSSVRRDVLLRGMKVKGEAAVRMGI 180
           Y+FMR DRGVLYMSEVD+GLS+PDYF A+ +SKIG+S+ RR+VLL G K++GE AV +GI
Sbjct: 121 YIFMRKDRGVLYMSEVDIGLSMPDYFAAMVRSKIGTSAARREVLLSGKKIRGEEAVALGI 180

Query: 181 VES-AHDGEDGVMKAAVRLGEQLGARNWNGEPYAEIRKSLHPEISGLLGLPEKVISISKL 240
           V+S AHD  +GV++A V L E+L A+ WNG+ YA IRKSL+PE+  +LGL   + +  KL
Sbjct: 181 VDSAAHDSAEGVVEATVSLAEKLAAKKWNGDVYASIRKSLYPELCEILGLKATIFATPKL 240

[Close]

BLAST of Lsi05G017020.1 vs. TAIR10
Match: AT4G14430.1 (AT4G14430.1 indole-3-butyric acid response 10)

HSP 1 Score: 317.4 bits (812), Expect = 7.6e-87
Identity = 159/240 (66.25%), Postives = 199/240 (82.92%), Query Frame = 1


Query: 1   MCTLEKRRNLFILTLTGDDDHRLSPSFIASILEALSKVKSQASRGSVLITTSHGKFFSNG 60
           MCTLEKR +LF+LTLTGD +HR  P  IA+IL  L + KSQ++RGS+LITT++GKFFSNG
Sbjct: 1   MCTLEKRGDLFLLTLTGDGEHRFHPDTIATILSLLEQAKSQSTRGSILITTANGKFFSNG 60

Query: 61  FDLSWAQAAGSVSAAAERLRHMVEIFKPVVAQLLSLPMPTIAAIAGHAAAAGFMLALSHD 120
           FDL+WAQ AGS + AA RL  MVE FKPVVA LL LPMPTIAA+ GHAAAAG +LALSHD
Sbjct: 61  FDLAWAQTAGSKTGAANRLHQMVESFKPVVAALLDLPMPTIAALNGHAAAAGLILALSHD 120

Query: 121 YLFMRSDRGVLYMSEVDLGLSLPDYFTALFKSKIGSSSVRRDVLLRGMKVKGEAAVRMGI 180
           Y+FMR DRGVLYMSEVD+GLS+PDYF+AL ++KIG+S+ RR++LL G K++GE AV +GI
Sbjct: 121 YVFMRKDRGVLYMSEVDIGLSMPDYFSALVRAKIGTSAARRELLLSGKKIRGEEAVGLGI 180

Query: 181 VES-AHDGEDGVMKAAVRLGEQLGARNWNGEPYAEIRKSLHPEISGLLGLPEKVISISKL 240
           V+S A+D E+GV+ A+VRLGE+L A+ W+GE YA IRKSL+PE+ G+LGL  +V +  KL
Sbjct: 181 VDSAAYDSEEGVVVASVRLGEKLAAKKWSGEVYASIRKSLYPELCGILGLETRVFATPKL 240

[Close]

BLAST of Lsi05G017020.1 vs. TAIR10
Match: AT4G14440.1 (AT4G14440.1 3-hydroxyacyl-CoA dehydratase 1)

HSP 1 Score: 292.0 bits (746), Expect = 3.4e-79
Identity = 147/230 (63.91%), Postives = 183/230 (79.57%), Query Frame = 1


Query: 1   MCTLEKRRNLFILTLTGDDDHRLSPSFIASILEALSKVKSQASRGSVLITTSHGKFFSNG 60
           MCTLEKR +LF+LTLTG+D+HR  P  IAS+L  L + KSQ+++GSVLITT HGKFFSNG
Sbjct: 1   MCTLEKRGDLFLLTLTGEDEHRFHPDTIASVLSLLEQAKSQSTKGSVLITTGHGKFFSNG 60

Query: 61  FDLSWAQAAGSVSAAAERLRHMVEIFKPVVAQLLSLPMPTIAAIAGHAAAAGFMLALSHD 120
           FDL+WAQ+AG    A +R+  MV+ FKPV+A LL LPMPTIAA+ GHAAA+G M ALSHD
Sbjct: 61  FDLAWAQSAGH--GAIKRMHQMVKSFKPVLAALLDLPMPTIAALNGHAAASGLMFALSHD 120

Query: 121 YLFMRSDRGVLYMSEVDLGLSLPDYFTALFKSKIGSSSVRRDVLLRGMKVKGEAAVRMGI 180
           Y+FMR DRGVLYMSEVD+GL +PDYF+AL  +K+GS   RR++LL G K+KGE AV +GI
Sbjct: 121 YVFMRKDRGVLYMSEVDIGLPVPDYFSALVVAKVGSGIARRELLLSGKKLKGEEAVALGI 180

Query: 181 VES-AHDGEDGVMKAAVRLGEQLGARNWNGEPYAEIRKSLHPEISGLLGL 230
           V+S AHD  +GV++A V LGE L A+ WNGE YA IRKSL+PE+  ++ L
Sbjct: 181 VDSAAHDSAEGVVEATVSLGESLAAKKWNGEVYATIRKSLYPELCRMVDL 228

[Close]

BLAST of Lsi05G017020.1 vs. TAIR10
Match: AT1G65520.1 (AT1G65520.1 delta(3), delta(2)-enoyl CoA isomerase 1)

HSP 1 Score: 206.1 bits (523), Expect = 2.5e-53
Identity = 109/224 (48.66%), Postives = 157/224 (70.09%), Query Frame = 1


Query: 1   MCTLEKRRNLFILTLTGDDDHRLSPSFIASILEALSKVKSQASRG-SVLITTSHGKFFSN 60
           MC+LEKR  LFIL LTGD +HRL+P+ + S+   +++++S  S   SVLITTS GKFFSN
Sbjct: 1   MCSLEKRDRLFILKLTGDGEHRLNPTLLDSLRSTINQIRSDPSFSQSVLITTSDGKFFSN 60

Query: 61  GFDLSWAQAAGSVSAAAERLRHMVEIFKPVVAQLLSLPMPTIAAIAGHAAAAGFMLALSH 120
           G+DL+ A++  S+S   +         + +VA L+SLPMPTIAA+ GHA+AAG +LA+SH
Sbjct: 61  GYDLALAESNPSLSVVMDAK------LRSLVADLISLPMPTIAAVTGHASAAGCILAMSH 120

Query: 121 DYLFMRSDRGVLYMSEVDLGLSLPDYFTALFKSKIGSSSVRRDVLLRGMKVKGEAAVRMG 180
           DY+ MR DRG LYMSE+D+ L +P +F A+ + KIGS + RRDV+L   KV  +  V+MG
Sbjct: 121 DYVLMRRDRGFLYMSELDIELIVPAWFMAVIRGKIGSPAARRDVMLTAAKVTADVGVKMG 180

Query: 181 IVESAHDGEDGVMKAAVRLGEQLGARNWNGEPYAEIRKSLHPEI 224
           IV+SA+      ++AA++LGE++  R  +G  Y ++R+SL  E+
Sbjct: 181 IVDSAYGSAAETVEAAIKLGEEIVQRGGDGHVYGKMRESLLREV 218

[Close]

BLAST of Lsi05G017020.1 vs. NCBI nr
Match: gi|449432586|ref|XP_004134080.1| (PREDICTED: 3-hydroxybutyryl-CoA dehydratase-like protein, mitochondrial [Cucumis sativus])

HSP 1 Score: 416.4 bits (1069), Expect = 3.4e-113
Identity = 215/239 (89.96%), Postives = 226/239 (94.56%), Query Frame = 1


Query: 1   MCTLEKRRNLFILTLTGDDDHRLSPSFIASILEALSKVKSQASRGSVLITTSHGKFFSNG 60
           MCTLEKRRNLFILTLTG DDHR SPS IA+ILEALSKVKSQAS GSVLITTSHGKFFSNG
Sbjct: 1   MCTLEKRRNLFILTLTGHDDHRFSPSVIAAILEALSKVKSQASTGSVLITTSHGKFFSNG 60

Query: 61  FDLSWAQAAGSVSAAAERLRHMVEIFKPVVAQLLSLPMPTIAAIAGHAAAAGFMLALSHD 120
           FDLSWAQAAGSVSAAAERL HMV+IFKPVVA+LLSLPMPTIAAI+GHAAAAGF+LALSHD
Sbjct: 61  FDLSWAQAAGSVSAAAERLHHMVQIFKPVVAELLSLPMPTIAAISGHAAAAGFLLALSHD 120

Query: 121 YLFMRSDRGVLYMSEVDLGLSLPDYFTALFKSKIGSSSVRRDVLLRGMKVKGEAAVRMGI 180
           YL MRSDRGVLYMSEVDLGLSLPDYF ALFKSKI SSSVRRDVL+RGMKVKGEAAV+MGI
Sbjct: 121 YLLMRSDRGVLYMSEVDLGLSLPDYFAALFKSKIASSSVRRDVLMRGMKVKGEAAVKMGI 180

Query: 181 VESAHDGEDGVMKAAVRLGEQLGARNWNGEPYAEIRKSLHPEISGLLGLPEKVISISKL 240
            +SAHDGE+GVM+AAVRLGEQLG R WNGEPYAEIRKSL+PEIS LLGLPEK ISISKL
Sbjct: 181 ADSAHDGENGVMEAAVRLGEQLGERKWNGEPYAEIRKSLYPEISRLLGLPEKAISISKL 239

[Close]

BLAST of Lsi05G017020.1 vs. NCBI nr
Match: gi|659076208|ref|XP_008438558.1| (PREDICTED: enoyl-CoA hydratase domain-containing protein 2, mitochondrial-like [Cucumis melo])

HSP 1 Score: 413.3 bits (1061), Expect = 2.9e-112
Identity = 213/239 (89.12%), Postives = 226/239 (94.56%), Query Frame = 1


Query: 1   MCTLEKRRNLFILTLTGDDDHRLSPSFIASILEALSKVKSQASRGSVLITTSHGKFFSNG 60
           MCTLEKRRNLFILTLTGD DHRL+PS IA++LEALSKVKSQAS GSVLITTSHGKFFSNG
Sbjct: 1   MCTLEKRRNLFILTLTGDHDHRLNPSVIAALLEALSKVKSQASPGSVLITTSHGKFFSNG 60

Query: 61  FDLSWAQAAGSVSAAAERLRHMVEIFKPVVAQLLSLPMPTIAAIAGHAAAAGFMLALSHD 120
           FDLSWA AAGSVSAAAERL HMV+ FKPV+A+LLSLPMPTIAAI+GHAAAAGFMLALSHD
Sbjct: 61  FDLSWAHAAGSVSAAAERLHHMVQNFKPVIAELLSLPMPTIAAISGHAAAAGFMLALSHD 120

Query: 121 YLFMRSDRGVLYMSEVDLGLSLPDYFTALFKSKIGSSSVRRDVLLRGMKVKGEAAVRMGI 180
           YL MRSDRGVLYMSEVDLGLSLPDYF ALFKSKIGSSSVRRDVL+RGMKVKGEAAV+MGI
Sbjct: 121 YLLMRSDRGVLYMSEVDLGLSLPDYFAALFKSKIGSSSVRRDVLMRGMKVKGEAAVKMGI 180

Query: 181 VESAHDGEDGVMKAAVRLGEQLGARNWNGEPYAEIRKSLHPEISGLLGLPEKVISISKL 240
            +SA+DGE GVM+AAVRLGEQLGAR WNGEPYAEIRKSL+PEIS LLGLPEK ISISKL
Sbjct: 181 ADSAYDGESGVMEAAVRLGEQLGARKWNGEPYAEIRKSLYPEISRLLGLPEKAISISKL 239

[Close]

BLAST of Lsi05G017020.1 vs. NCBI nr
Match: gi|449432584|ref|XP_004134079.1| (PREDICTED: 3-hydroxybutyryl-CoA dehydratase-like protein, mitochondrial [Cucumis sativus])

HSP 1 Score: 371.7 bits (953), Expect = 9.6e-100
Identity = 191/239 (79.92%), Postives = 211/239 (88.28%), Query Frame = 1


Query: 1   MCTLEKRRNLFILTLTGDDDHRLSPSFIASILEALSKVKSQASRGSVLITTSHGKFFSNG 60
           MCT+EKR N FILTLTGDDDHRLSPS I SIL+ALS+VK+QAS  SVLITTSHGKFFSNG
Sbjct: 1   MCTVEKRGNFFILTLTGDDDHRLSPSLINSILKALSQVKAQASPASVLITTSHGKFFSNG 60

Query: 61  FDLSWAQAAGSVSAAAERLRHMVEIFKPVVAQLLSLPMPTIAAIAGHAAAAGFMLALSHD 120
           FDL WA +AGSVSAA  RL H++EIFK VVA+L+SLPMPTIAAI GHAAAAGF LALSHD
Sbjct: 61  FDLPWALSAGSVSAARHRLNHLIEIFKSVVAELISLPMPTIAAITGHAAAAGFTLALSHD 120

Query: 121 YLFMRSDRGVLYMSEVDLGLSLPDYFTALFKSKIGSSSVRRDVLLRGMKVKGEAAVRMGI 180
           YL MRSDRGVLYM E+DLGL+LPDYF ALFKSKIGSS   RD++LRGMKV+GEAAVRMGI
Sbjct: 121 YLIMRSDRGVLYMRELDLGLTLPDYFMALFKSKIGSSLAMRDLILRGMKVRGEAAVRMGI 180

Query: 181 VESAHDGEDGVMKAAVRLGEQLGARNWNGEPYAEIRKSLHPEISGLLGLPEKVISISKL 240
           V+SAHDGED V+ AAVRLGEQL  RNW+GE YAEIRKSL+PE+S +LGLP K ISISKL
Sbjct: 181 VDSAHDGEDSVVNAAVRLGEQLAGRNWDGEVYAEIRKSLYPEVSEVLGLPGKPISISKL 239

[Close]

BLAST of Lsi05G017020.1 vs. NCBI nr
Match: gi|659076193|ref|XP_008438552.1| (PREDICTED: enoyl-CoA hydratase domain-containing protein 2, mitochondrial-like [Cucumis melo])

HSP 1 Score: 364.0 bits (933), Expect = 2.0e-97
Identity = 190/239 (79.50%), Postives = 208/239 (87.03%), Query Frame = 1


Query: 1   MCTLEKRRNLFILTLTGDDDHRLSPSFIASILEALSKVKSQASRGSVLITTSHGKFFSNG 60
           MCT+EKR N FILTLTGDDDHRLSPS I SIL+ALS+VK+QAS  SVLITTSHGKFFSNG
Sbjct: 1   MCTVEKRGNFFILTLTGDDDHRLSPSLINSILKALSQVKAQASPASVLITTSHGKFFSNG 60

Query: 61  FDLSWAQAAGSVSAAAERLRHMVEIFKPVVAQLLSLPMPTIAAIAGHAAAAGFMLALSHD 120
           FDL WA AAGSVSAA  RL H++EIFK VVA+L+SLPMPTIAAI GHAAAAGF LALSHD
Sbjct: 61  FDLPWALAAGSVSAARHRLNHIIEIFKSVVAELISLPMPTIAAITGHAAAAGFTLALSHD 120

Query: 121 YLFMRSDRGVLYMSEVDLGLSLPDYFTALFKSKIGSSSVRRDVLLRGMKVKGEAAVRMGI 180
           YL MRSDRGVLYM E+DLGL+LPDYF ALFKSKIGSS   RD++LRGMKV+GEAAVRMGI
Sbjct: 121 YLIMRSDRGVLYMRELDLGLTLPDYFMALFKSKIGSSLAMRDLILRGMKVRGEAAVRMGI 180

Query: 181 VESAHDGEDGVMKAAVRLGEQLGARNWNGEPYAEIRKSLHPEISGLLGLPEKVISISKL 240
           V+SAH  E  V+ AAVRLGEQL  RNW+GE YAEIRKSL+PEIS +LGLP K ISISKL
Sbjct: 181 VDSAHHSEVSVVNAAVRLGEQLVGRNWDGEVYAEIRKSLYPEISEVLGLPGKAISISKL 239

[Close]

BLAST of Lsi05G017020.1 vs. NCBI nr
Match: gi|449432588|ref|XP_004134081.1| (PREDICTED: 3-hydroxybutyryl-CoA dehydratase-like protein, mitochondrial [Cucumis sativus])

HSP 1 Score: 339.3 bits (869), Expect = 5.3e-90
Identity = 175/241 (72.61%), Postives = 205/241 (85.06%), Query Frame = 1


Query: 1   MCTLEKRRNLFILTLTGDDDHRLSPSFIASILEALSKVKSQ--ASRGSVLITTSHGKFFS 60
           MC++EKR  LFILTLTG+ +H L+PS I SIL ALS+VK++  AS GSVLITTS GKFFS
Sbjct: 1   MCSMEKRGKLFILTLTGNHEHWLNPSLINSILNALSQVKAEARASPGSVLITTSQGKFFS 60

Query: 61  NGFDLSWAQAAGSVSAAAERLRHMVEIFKPVVAQLLSLPMPTIAAIAGHAAAAGFMLALS 120
           NG DL W  +A S+SAA  RL HM+++FKP++A LL LP+PTIAA+ GHAAA G +LAL+
Sbjct: 61  NGLDLPWILSASSISAAHNRLNHMIQLFKPLLAHLLCLPIPTIAALPGHAAAGGLVLALA 120

Query: 121 HDYLFMRSDRGVLYMSEVDLGLSLPDYFTALFKSKIGSSSVRRDVLLRGMKVKGEAAVRM 180
           HDYL MRSDRGVLYMSE+DLG +LPDYF AL KSKIGSSSVRRDV LRGMKV+GE AV+M
Sbjct: 121 HDYLVMRSDRGVLYMSELDLGATLPDYFIALAKSKIGSSSVRRDVFLRGMKVRGETAVKM 180

Query: 181 GIVESAHDGEDGVMKAAVRLGEQLGARNWNGEPYAEIRKSLHPEISGLLGLPEKVISISK 240
           G+ ESAH GEDGVM+AAVRLGE+L ARNW+G+ YAEIRKSL+PEISGLLGL  KVI+ISK
Sbjct: 181 GLAESAHQGEDGVMEAAVRLGEELAARNWDGDVYAEIRKSLYPEISGLLGLTTKVITISK 240

The following BLAST results are available for this feature:

BLAST of Lsi05G017020.1 vs. Swiss-Prot


Match Name	E-value	Identity	Description
ECI2_ARATH	1.3e-85	66.25	Enoyl-CoA delta isomerase 2, peroxisomal OS=Arabidopsis thaliana GN=ECI2 PE=1 SV...	[more]
ECI3_ARATH	6.1e-78	63.91	Enoyl-CoA delta isomerase 3 OS=Arabidopsis thaliana GN=ECI3 PE=1 SV=1	[more]
ECI1_ARATH	4.4e-52	48.66	Enoyl-CoA delta isomerase 1, peroxisomal OS=Arabidopsis thaliana GN=ECI1 PE=1 SV...	[more]

BLAST of Lsi05G017020.1 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L8C7_CUCSA	2.4e-113	89.96	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G141780 PE=4 SV=1	[more]
A0A0A0L9X4_CUCSA	6.7e-100	79.92	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G141770 PE=4 SV=1	[more]
M5XMU9_PRUPE	3.8e-87	72.49	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa010715mg PE=4 SV=1	[more]
V4MKX3_EUTSA	1.1e-86	67.92	Uncharacterized protein OS=Eutrema salsugineum GN=EUTSA_v10027007mg PE=4 SV=1	[more]
A0A0D3DLS0_BRAOL	2.1e-85	67.50	Uncharacterized protein OS=Brassica oleracea var. oleracea GN=IBR10 PE=4 SV=1	[more]

BLAST of Lsi05G017020.1 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G14430.1	7.6e-87	66.25	indole-3-butyric acid response 10	[more]
AT4G14440.1	3.4e-79	63.91	3-hydroxyacyl-CoA dehydratase 1	[more]
AT1G65520.1	2.5e-53	48.66	delta(3), delta(2)-enoyl CoA isomerase 1	[more]

BLAST of Lsi05G017020.1 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449432586\|ref\|XP_004134080.1\|	3.4e-113	89.96	PREDICTED: 3-hydroxybutyryl-CoA dehydratase-like protein, mitochondrial [Cucumis...	[more]
gi\|659076208\|ref\|XP_008438558.1\|	2.9e-112	89.12	PREDICTED: enoyl-CoA hydratase domain-containing protein 2, mitochondrial-like [...	[more]
gi\|449432584\|ref\|XP_004134079.1\|	9.6e-100	79.92	PREDICTED: 3-hydroxybutyryl-CoA dehydratase-like protein, mitochondrial [Cucumis...	[more]
gi\|659076193\|ref\|XP_008438552.1\|	2.0e-97	79.50	PREDICTED: enoyl-CoA hydratase domain-containing protein 2, mitochondrial-like [...	[more]
gi\|449432588\|ref\|XP_004134081.1\|	5.3e-90	72.61	PREDICTED: 3-hydroxybutyryl-CoA dehydratase-like protein, mitochondrial [Cucumis...	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **Biological Process**
Term	Definition
GO:0008152	metabolic process

Vocabulary: **Molecular Function**
Term	Definition
GO:0003824	catalytic activity

Vocabulary: **INTERPRO**
Term	Definition
IPR001753	Enoyl-CoA_hydra/iso

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008152	metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0003824	catalytic activity
molecular_function	GO:0016853	isomerase activity

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Lsi05G017020	Lsi05G017020	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lsi05G017020.1.CDS.1	Lsi05G017020.1.CDS.1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Lsi05G017020.1	Lsi05G017020.1-protein	polypeptide

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001753	Crotonase superfamily	PFAM	PF00378	ECH_1	coord: 23..203 score: 5.5
None	No IPR available	PANTHER	PTHR11941	ENOYL-COA HYDRATASE-RELATED	coord: 1..223 score: 8.4
None	No IPR available	PANTHER	PTHR11941:SF84	SUBFAMILY NOT NAMED	coord: 1..223 score: 8.4