Lsi05G017020 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi05G017020
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Enoyl-CoA hydratase/isomerase family protein
Location	chr05 : 24564668 .. 24565387 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTGCACACTCGAGAAGCGACGCAATCTTTTCATCCTAACTCTGACCGGCGACGACGACCACCGCCTCAGCCCCTCCTTCATCGCCTCCATCCTCGAGGCTCTTTCCAAAGTCAAATCCCAAGCCTCCCGTGGCTCCGTTCTCATCACTACTTCCCATGGCAAATTCTTCTCTAACGGCTTCGATCTCTCCTGGGCTCAAGCCGCCGGCTCTGTTTCAGCCGCCGCCGAACGCCTCAGGCATATGGTCGAGATTTTCAAGCCCGTCGTCGCCCAACTCCTCTCCCTCCCCATGCCCACCATCGCTGCCATTGCAGGCCACGCCGCCGCGGCCGGCTTCATGTTAGCCCTCAGTCACGACTATTTGTTCATGAGGAGCGATCGAGGCGTTCTGTACATGAGCGAAGTGGATCTAGGGCTAAGTCTGCCCGATTACTTCACGGCGCTGTTTAAATCGAAGATCGGCTCGAGTTCCGTCCGCCGCGATGTGCTCTTGAGGGGAATGAAAGTGAAAGGTGAAGCGGCGGTGAGGATGGGGATCGTGGAATCGGCGCATGACGGAGAGGACGGTGTCATGAAGGCTGCCGTACGACTCGGGGAGCAGTTGGGAGCGAGAAATTGGAACGGCGAACCCTACGCAGAAATTAGGAAAAGTCTTCATCCAGAGATCTCTGGGCTGCTTGGACTGCCGGAAAAGGTCATATCGATTTCCAAGCTATGA

mRNA sequence

ATGTGCACACTCGAGAAGCGACGCAATCTTTTCATCCTAACTCTGACCGGCGACGACGACCACCGCCTCAGCCCCTCCTTCATCGCCTCCATCCTCGAGGCTCTTTCCAAAGTCAAATCCCAAGCCTCCCGTGGCTCCGTTCTCATCACTACTTCCCATGGCAAATTCTTCTCTAACGGCTTCGATCTCTCCTGGGCTCAAGCCGCCGGCTCTGTTTCAGCCGCCGCCGAACGCCTCAGGCATATGGTCGAGATTTTCAAGCCCGTCGTCGCCCAACTCCTCTCCCTCCCCATGCCCACCATCGCTGCCATTGCAGGCCACGCCGCCGCGGCCGGCTTCATGTTAGCCCTCAGTCACGACTATTTGTTCATGAGGAGCGATCGAGGCGTTCTGTACATGAGCGAAGTGGATCTAGGGCTAAGTCTGCCCGATTACTTCACGGCGCTGTTTAAATCGAAGATCGGCTCGAGTTCCGTCCGCCGCGATGTGCTCTTGAGGGGAATGAAAGTGAAAGGTGAAGCGGCGGTGAGGATGGGGATCGTGGAATCGGCGCATGACGGAGAGGACGGTGTCATGAAGGCTGCCGTACGACTCGGGGAGCAGTTGGGAGCGAGAAATTGGAACGGCGAACCCTACGCAGAAATTAGGAAAAGTCTTCATCCAGAGATCTCTGGGCTGCTTGGACTGCCGGAAAAGGTCATATCGATTTCCAAGCTATGA

Coding sequence (CDS)

ATGTGCACACTCGAGAAGCGACGCAATCTTTTCATCCTAACTCTGACCGGCGACGACGACCACCGCCTCAGCCCCTCCTTCATCGCCTCCATCCTCGAGGCTCTTTCCAAAGTCAAATCCCAAGCCTCCCGTGGCTCCGTTCTCATCACTACTTCCCATGGCAAATTCTTCTCTAACGGCTTCGATCTCTCCTGGGCTCAAGCCGCCGGCTCTGTTTCAGCCGCCGCCGAACGCCTCAGGCATATGGTCGAGATTTTCAAGCCCGTCGTCGCCCAACTCCTCTCCCTCCCCATGCCCACCATCGCTGCCATTGCAGGCCACGCCGCCGCGGCCGGCTTCATGTTAGCCCTCAGTCACGACTATTTGTTCATGAGGAGCGATCGAGGCGTTCTGTACATGAGCGAAGTGGATCTAGGGCTAAGTCTGCCCGATTACTTCACGGCGCTGTTTAAATCGAAGATCGGCTCGAGTTCCGTCCGCCGCGATGTGCTCTTGAGGGGAATGAAAGTGAAAGGTGAAGCGGCGGTGAGGATGGGGATCGTGGAATCGGCGCATGACGGAGAGGACGGTGTCATGAAGGCTGCCGTACGACTCGGGGAGCAGTTGGGAGCGAGAAATTGGAACGGCGAACCCTACGCAGAAATTAGGAAAAGTCTTCATCCAGAGATCTCTGGGCTGCTTGGACTGCCGGAAAAGGTCATATCGATTTCCAAGCTATGA

Protein sequence

MCTLEKRRNLFILTLTGDDDHRLSPSFIASILEALSKVKSQASRGSVLITTSHGKFFSNGFDLSWAQAAGSVSAAAERLRHMVEIFKPVVAQLLSLPMPTIAAIAGHAAAAGFMLALSHDYLFMRSDRGVLYMSEVDLGLSLPDYFTALFKSKIGSSSVRRDVLLRGMKVKGEAAVRMGIVESAHDGEDGVMKAAVRLGEQLGARNWNGEPYAEIRKSLHPEISGLLGLPEKVISISKL

Homology

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BLAST of Lsi05G017020 vs. Swiss-Prot
Match: ECI2_ARATH (Enoyl-CoA delta isomerase 2, peroxisomal OS=Arabidopsis thaliana GN=ECI2 PE=1 SV=1)

HSP 1 Score: 317.4 bits (812), Expect = 1.3e-85
Identity = 159/240 (66.25%), Postives = 199/240 (82.92%), Query Frame = 1


Query: 1   MCTLEKRRNLFILTLTGDDDHRLSPSFIASILEALSKVKSQASRGSVLITTSHGKFFSNG 60
           MCTLEKR +LF+LTLTGD +HR  P  IA+IL  L + KSQ++RGS+LITT++GKFFSNG
Sbjct: 1   MCTLEKRGDLFLLTLTGDGEHRFHPDTIATILSLLEQAKSQSTRGSILITTANGKFFSNG 60

Query: 61  FDLSWAQAAGSVSAAAERLRHMVEIFKPVVAQLLSLPMPTIAAIAGHAAAAGFMLALSHD 120
           FDL+WAQ AGS + AA RL  MVE FKPVVA LL LPMPTIAA+ GHAAAAG +LALSHD
Sbjct: 61  FDLAWAQTAGSKTGAANRLHQMVESFKPVVAALLDLPMPTIAALNGHAAAAGLILALSHD 120

Query: 121 YLFMRSDRGVLYMSEVDLGLSLPDYFTALFKSKIGSSSVRRDVLLRGMKVKGEAAVRMGI 180
           Y+FMR DRGVLYMSEVD+GLS+PDYF+AL ++KIG+S+ RR++LL G K++GE AV +GI
Sbjct: 121 YVFMRKDRGVLYMSEVDIGLSMPDYFSALVRAKIGTSAARRELLLSGKKIRGEEAVGLGI 180

Query: 181 VES-AHDGEDGVMKAAVRLGEQLGARNWNGEPYAEIRKSLHPEISGLLGLPEKVISISKL 240
           V+S A+D E+GV+ A+VRLGE+L A+ W+GE YA IRKSL+PE+ G+LGL  +V +  KL
Sbjct: 181 VDSAAYDSEEGVVVASVRLGEKLAAKKWSGEVYASIRKSLYPELCGILGLETRVFATPKL 240

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BLAST of Lsi05G017020 vs. Swiss-Prot
Match: ECI3_ARATH (Enoyl-CoA delta isomerase 3 OS=Arabidopsis thaliana GN=ECI3 PE=1 SV=1)

HSP 1 Score: 292.0 bits (746), Expect = 6.1e-78
Identity = 147/230 (63.91%), Postives = 183/230 (79.57%), Query Frame = 1


Query: 1   MCTLEKRRNLFILTLTGDDDHRLSPSFIASILEALSKVKSQASRGSVLITTSHGKFFSNG 60
           MCTLEKR +LF+LTLTG+D+HR  P  IAS+L  L + KSQ+++GSVLITT HGKFFSNG
Sbjct: 1   MCTLEKRGDLFLLTLTGEDEHRFHPDTIASVLSLLEQAKSQSTKGSVLITTGHGKFFSNG 60

Query: 61  FDLSWAQAAGSVSAAAERLRHMVEIFKPVVAQLLSLPMPTIAAIAGHAAAAGFMLALSHD 120
           FDL+WAQ+AG    A +R+  MV+ FKPV+A LL LPMPTIAA+ GHAAA+G M ALSHD
Sbjct: 61  FDLAWAQSAGH--GAIKRMHQMVKSFKPVLAALLDLPMPTIAALNGHAAASGLMFALSHD 120

Query: 121 YLFMRSDRGVLYMSEVDLGLSLPDYFTALFKSKIGSSSVRRDVLLRGMKVKGEAAVRMGI 180
           Y+FMR DRGVLYMSEVD+GL +PDYF+AL  +K+GS   RR++LL G K+KGE AV +GI
Sbjct: 121 YVFMRKDRGVLYMSEVDIGLPVPDYFSALVVAKVGSGIARRELLLSGKKLKGEEAVALGI 180

Query: 181 VES-AHDGEDGVMKAAVRLGEQLGARNWNGEPYAEIRKSLHPEISGLLGL 230
           V+S AHD  +GV++A V LGE L A+ WNGE YA IRKSL+PE+  ++ L
Sbjct: 181 VDSAAHDSAEGVVEATVSLGESLAAKKWNGEVYATIRKSLYPELCRMVDL 228

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BLAST of Lsi05G017020 vs. Swiss-Prot
Match: ECI1_ARATH (Enoyl-CoA delta isomerase 1, peroxisomal OS=Arabidopsis thaliana GN=ECI1 PE=1 SV=1)

HSP 1 Score: 206.1 bits (523), Expect = 4.4e-52
Identity = 109/224 (48.66%), Postives = 157/224 (70.09%), Query Frame = 1


Query: 1   MCTLEKRRNLFILTLTGDDDHRLSPSFIASILEALSKVKSQASRG-SVLITTSHGKFFSN 60
           MC+LEKR  LFIL LTGD +HRL+P+ + S+   +++++S  S   SVLITTS GKFFSN
Sbjct: 1   MCSLEKRDRLFILKLTGDGEHRLNPTLLDSLRSTINQIRSDPSFSQSVLITTSDGKFFSN 60

Query: 61  GFDLSWAQAAGSVSAAAERLRHMVEIFKPVVAQLLSLPMPTIAAIAGHAAAAGFMLALSH 120
           G+DL+ A++  S+S   +         + +VA L+SLPMPTIAA+ GHA+AAG +LA+SH
Sbjct: 61  GYDLALAESNPSLSVVMDAK------LRSLVADLISLPMPTIAAVTGHASAAGCILAMSH 120

Query: 121 DYLFMRSDRGVLYMSEVDLGLSLPDYFTALFKSKIGSSSVRRDVLLRGMKVKGEAAVRMG 180
           DY+ MR DRG LYMSE+D+ L +P +F A+ + KIGS + RRDV+L   KV  +  V+MG
Sbjct: 121 DYVLMRRDRGFLYMSELDIELIVPAWFMAVIRGKIGSPAARRDVMLTAAKVTADVGVKMG 180

Query: 181 IVESAHDGEDGVMKAAVRLGEQLGARNWNGEPYAEIRKSLHPEI 224
           IV+SA+      ++AA++LGE++  R  +G  Y ++R+SL  E+
Sbjct: 181 IVDSAYGSAAETVEAAIKLGEEIVQRGGDGHVYGKMRESLLREV 218

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BLAST of Lsi05G017020 vs. TrEMBL
Match: A0A0A0L8C7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G141780 PE=4 SV=1)

HSP 1 Score: 416.4 bits (1069), Expect = 2.4e-113
Identity = 215/239 (89.96%), Postives = 226/239 (94.56%), Query Frame = 1


Query: 1   MCTLEKRRNLFILTLTGDDDHRLSPSFIASILEALSKVKSQASRGSVLITTSHGKFFSNG 60
           MCTLEKRRNLFILTLTG DDHR SPS IA+ILEALSKVKSQAS GSVLITTSHGKFFSNG
Sbjct: 1   MCTLEKRRNLFILTLTGHDDHRFSPSVIAAILEALSKVKSQASTGSVLITTSHGKFFSNG 60

Query: 61  FDLSWAQAAGSVSAAAERLRHMVEIFKPVVAQLLSLPMPTIAAIAGHAAAAGFMLALSHD 120
           FDLSWAQAAGSVSAAAERL HMV+IFKPVVA+LLSLPMPTIAAI+GHAAAAGF+LALSHD
Sbjct: 61  FDLSWAQAAGSVSAAAERLHHMVQIFKPVVAELLSLPMPTIAAISGHAAAAGFLLALSHD 120

Query: 121 YLFMRSDRGVLYMSEVDLGLSLPDYFTALFKSKIGSSSVRRDVLLRGMKVKGEAAVRMGI 180
           YL MRSDRGVLYMSEVDLGLSLPDYF ALFKSKI SSSVRRDVL+RGMKVKGEAAV+MGI
Sbjct: 121 YLLMRSDRGVLYMSEVDLGLSLPDYFAALFKSKIASSSVRRDVLMRGMKVKGEAAVKMGI 180

Query: 181 VESAHDGEDGVMKAAVRLGEQLGARNWNGEPYAEIRKSLHPEISGLLGLPEKVISISKL 240
            +SAHDGE+GVM+AAVRLGEQLG R WNGEPYAEIRKSL+PEIS LLGLPEK ISISKL
Sbjct: 181 ADSAHDGENGVMEAAVRLGEQLGERKWNGEPYAEIRKSLYPEISRLLGLPEKAISISKL 239

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BLAST of Lsi05G017020 vs. TrEMBL
Match: A0A0A0L9X4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G141770 PE=4 SV=1)

HSP 1 Score: 371.7 bits (953), Expect = 6.7e-100
Identity = 191/239 (79.92%), Postives = 211/239 (88.28%), Query Frame = 1


Query: 1   MCTLEKRRNLFILTLTGDDDHRLSPSFIASILEALSKVKSQASRGSVLITTSHGKFFSNG 60
           MCT+EKR N FILTLTGDDDHRLSPS I SIL+ALS+VK+QAS  SVLITTSHGKFFSNG
Sbjct: 1   MCTVEKRGNFFILTLTGDDDHRLSPSLINSILKALSQVKAQASPASVLITTSHGKFFSNG 60

Query: 61  FDLSWAQAAGSVSAAAERLRHMVEIFKPVVAQLLSLPMPTIAAIAGHAAAAGFMLALSHD 120
           FDL WA +AGSVSAA  RL H++EIFK VVA+L+SLPMPTIAAI GHAAAAGF LALSHD
Sbjct: 61  FDLPWALSAGSVSAARHRLNHLIEIFKSVVAELISLPMPTIAAITGHAAAAGFTLALSHD 120

Query: 121 YLFMRSDRGVLYMSEVDLGLSLPDYFTALFKSKIGSSSVRRDVLLRGMKVKGEAAVRMGI 180
           YL MRSDRGVLYM E+DLGL+LPDYF ALFKSKIGSS   RD++LRGMKV+GEAAVRMGI
Sbjct: 121 YLIMRSDRGVLYMRELDLGLTLPDYFMALFKSKIGSSLAMRDLILRGMKVRGEAAVRMGI 180

Query: 181 VESAHDGEDGVMKAAVRLGEQLGARNWNGEPYAEIRKSLHPEISGLLGLPEKVISISKL 240
           V+SAHDGED V+ AAVRLGEQL  RNW+GE YAEIRKSL+PE+S +LGLP K ISISKL
Sbjct: 181 VDSAHDGEDSVVNAAVRLGEQLAGRNWDGEVYAEIRKSLYPEVSEVLGLPGKPISISKL 239

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BLAST of Lsi05G017020 vs. TrEMBL
Match: M5XMU9_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa010715mg PE=4 SV=1)

HSP 1 Score: 329.3 bits (843), Expect = 3.8e-87
Identity = 166/229 (72.49%), Postives = 192/229 (83.84%), Query Frame = 1


Query: 1   MCTLEKRRNLFILTLTGDDDHRLSPSFIASILEALSKVKSQASRGSVLITTSHGKFFSNG 60
           MCTLEKR +LF LTLT DD+HRLS + I S+L ALS+ KSQA+RGSVL+TT+HGKFFSNG
Sbjct: 1   MCTLEKRGDLFFLTLTCDDEHRLSLTLIDSLLSALSQAKSQATRGSVLVTTAHGKFFSNG 60

Query: 61  FDLSWAQAAGSVSAAAERLRHMVEIFKPVVAQLLSLPMPTIAAIAGHAAAAGFMLALSHD 120
           FDL WAQAAGS SAA  RL  MV  FKPVVA LLSLPMPT+AA+ GHAAAAGF+LALSHD
Sbjct: 61  FDLGWAQAAGSASAARARLSQMVAAFKPVVAALLSLPMPTVAAVQGHAAAAGFLLALSHD 120

Query: 121 YLFMRSDRGVLYMSEVDLGLSLPDYFTALFKSKIGSSSVRRDVLLRGMKVKGEAAVRMGI 180
           Y  MR DRGVLYMSEVDLGL  PDYFTA F +KIGS S RRDV+LRGMK+KG+ AV++GI
Sbjct: 121 YFLMRRDRGVLYMSEVDLGLPFPDYFTAAFLAKIGSVSGRRDVMLRGMKLKGDEAVKLGI 180

Query: 181 VESAHDGEDGVMKAAVRLGEQLGARNWNGEPYAEIRKSLHPEISGLLGL 230
           VESAHD  +  ++AAVRLGEQL  R WNG+ YAEIRKSL+PE+ G++G+
Sbjct: 181 VESAHDSAESTVEAAVRLGEQLAKRKWNGDVYAEIRKSLYPELCGVVGV 229

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BLAST of Lsi05G017020 vs. TrEMBL
Match: V4MKX3_EUTSA (Uncharacterized protein OS=Eutrema salsugineum GN=EUTSA_v10027007mg PE=4 SV=1)

HSP 1 Score: 327.8 bits (839), Expect = 1.1e-86
Identity = 163/240 (67.92%), Postives = 201/240 (83.75%), Query Frame = 1


Query: 1   MCTLEKRRNLFILTLTGDDDHRLSPSFIASILEALSKVKSQASRGSVLITTSHGKFFSNG 60
           MCTLEKR +LF+LTLTGD +HR  P+ IASI+  L + KSQ+ RGSVLITT++GKFFSNG
Sbjct: 48  MCTLEKRGDLFLLTLTGDGEHRFHPNTIASIISLLEQAKSQSKRGSVLITTANGKFFSNG 107

Query: 61  FDLSWAQAAGSVSAAAERLRHMVEIFKPVVAQLLSLPMPTIAAIAGHAAAAGFMLALSHD 120
           FDL+WAQ++GS +AAAERL HMVE FKPVVA LL LPMPTIAA+ GHAAAAG MLALSHD
Sbjct: 108 FDLAWAQSSGSKTAAAERLHHMVESFKPVVAALLDLPMPTIAALNGHAAAAGLMLALSHD 167

Query: 121 YLFMRSDRGVLYMSEVDLGLSLPDYFTALFKSKIGSSSVRRDVLLRGMKVKGEAAVRMGI 180
           Y+FMR+DRGVLYMSEVD+GLS+PDYF A+ +SKIG+S+ RR+V L G K++GE AV +GI
Sbjct: 168 YVFMRNDRGVLYMSEVDIGLSMPDYFAAIVRSKIGTSAARREVFLSGKKIRGEEAVALGI 227

Query: 181 VES-AHDGEDGVMKAAVRLGEQLGARNWNGEPYAEIRKSLHPEISGLLGLPEKVISISKL 240
           V+S AHD E+GV++A VRLGE+L A+ W+GE YA IRKSL+PE+ G+L L  K+ +  KL
Sbjct: 228 VDSAAHDSEEGVLEATVRLGEKLAAKKWSGEVYASIRKSLYPELCGMLNLEAKIFATPKL 287

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BLAST of Lsi05G017020 vs. TrEMBL
Match: A0A0D3DLS0_BRAOL (Uncharacterized protein OS=Brassica oleracea var. oleracea GN=IBR10 PE=4 SV=1)

HSP 1 Score: 323.6 bits (828), Expect = 2.1e-85
Identity = 162/240 (67.50%), Postives = 196/240 (81.67%), Query Frame = 1


Query: 1   MCTLEKRRNLFILTLTGDDDHRLSPSFIASILEALSKVKSQASRGSVLITTSHGKFFSNG 60
           MCTLEKR NLF+LTLTGD++HR +P  IA+IL  L + KSQA RGSVLITT HGKFFSNG
Sbjct: 1   MCTLEKRGNLFLLTLTGDNEHRFNPDAIATILSLLGQAKSQAKRGSVLITTGHGKFFSNG 60

Query: 61  FDLSWAQAAGSVSAAAERLRHMVEIFKPVVAQLLSLPMPTIAAIAGHAAAAGFMLALSHD 120
           FDL+WAQA+GS++ AAERL  MVE FKPVVA LL LPMPTIAA+ GHAAAAG MLALSHD
Sbjct: 61  FDLAWAQASGSLTGAAERLHQMVESFKPVVAALLDLPMPTIAALNGHAAAAGMMLALSHD 120

Query: 121 YLFMRSDRGVLYMSEVDLGLSLPDYFTALFKSKIGSSSVRRDVLLRGMKVKGEAAVRMGI 180
           Y+FMR DRGVLYMSEVD+GLS+PDYF A+ +SKIG+S+ RR+VLL G K++GE AV +GI
Sbjct: 121 YIFMRKDRGVLYMSEVDIGLSMPDYFAAMVRSKIGTSAARREVLLSGKKIRGEEAVALGI 180

Query: 181 VES-AHDGEDGVMKAAVRLGEQLGARNWNGEPYAEIRKSLHPEISGLLGLPEKVISISKL 240
           V+S AHD  +GV++A V L E+L A+ WNG+ YA IRKSL+PE+  +LGL   + +  KL
Sbjct: 181 VDSAAHDSAEGVVEATVSLAEKLAAKKWNGDVYASIRKSLYPELCEILGLKATIFATPKL 240

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BLAST of Lsi05G017020 vs. TAIR10
Match: AT4G14430.1 (AT4G14430.1 indole-3-butyric acid response 10)

HSP 1 Score: 317.4 bits (812), Expect = 7.6e-87
Identity = 159/240 (66.25%), Postives = 199/240 (82.92%), Query Frame = 1


Query: 1   MCTLEKRRNLFILTLTGDDDHRLSPSFIASILEALSKVKSQASRGSVLITTSHGKFFSNG 60
           MCTLEKR +LF+LTLTGD +HR  P  IA+IL  L + KSQ++RGS+LITT++GKFFSNG
Sbjct: 1   MCTLEKRGDLFLLTLTGDGEHRFHPDTIATILSLLEQAKSQSTRGSILITTANGKFFSNG 60

Query: 61  FDLSWAQAAGSVSAAAERLRHMVEIFKPVVAQLLSLPMPTIAAIAGHAAAAGFMLALSHD 120
           FDL+WAQ AGS + AA RL  MVE FKPVVA LL LPMPTIAA+ GHAAAAG +LALSHD
Sbjct: 61  FDLAWAQTAGSKTGAANRLHQMVESFKPVVAALLDLPMPTIAALNGHAAAAGLILALSHD 120

Query: 121 YLFMRSDRGVLYMSEVDLGLSLPDYFTALFKSKIGSSSVRRDVLLRGMKVKGEAAVRMGI 180
           Y+FMR DRGVLYMSEVD+GLS+PDYF+AL ++KIG+S+ RR++LL G K++GE AV +GI
Sbjct: 121 YVFMRKDRGVLYMSEVDIGLSMPDYFSALVRAKIGTSAARRELLLSGKKIRGEEAVGLGI 180

Query: 181 VES-AHDGEDGVMKAAVRLGEQLGARNWNGEPYAEIRKSLHPEISGLLGLPEKVISISKL 240
           V+S A+D E+GV+ A+VRLGE+L A+ W+GE YA IRKSL+PE+ G+LGL  +V +  KL
Sbjct: 181 VDSAAYDSEEGVVVASVRLGEKLAAKKWSGEVYASIRKSLYPELCGILGLETRVFATPKL 240

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BLAST of Lsi05G017020 vs. TAIR10
Match: AT4G14440.1 (AT4G14440.1 3-hydroxyacyl-CoA dehydratase 1)

HSP 1 Score: 292.0 bits (746), Expect = 3.4e-79
Identity = 147/230 (63.91%), Postives = 183/230 (79.57%), Query Frame = 1


Query: 1   MCTLEKRRNLFILTLTGDDDHRLSPSFIASILEALSKVKSQASRGSVLITTSHGKFFSNG 60
           MCTLEKR +LF+LTLTG+D+HR  P  IAS+L  L + KSQ+++GSVLITT HGKFFSNG
Sbjct: 1   MCTLEKRGDLFLLTLTGEDEHRFHPDTIASVLSLLEQAKSQSTKGSVLITTGHGKFFSNG 60

Query: 61  FDLSWAQAAGSVSAAAERLRHMVEIFKPVVAQLLSLPMPTIAAIAGHAAAAGFMLALSHD 120
           FDL+WAQ+AG    A +R+  MV+ FKPV+A LL LPMPTIAA+ GHAAA+G M ALSHD
Sbjct: 61  FDLAWAQSAGH--GAIKRMHQMVKSFKPVLAALLDLPMPTIAALNGHAAASGLMFALSHD 120

Query: 121 YLFMRSDRGVLYMSEVDLGLSLPDYFTALFKSKIGSSSVRRDVLLRGMKVKGEAAVRMGI 180
           Y+FMR DRGVLYMSEVD+GL +PDYF+AL  +K+GS   RR++LL G K+KGE AV +GI
Sbjct: 121 YVFMRKDRGVLYMSEVDIGLPVPDYFSALVVAKVGSGIARRELLLSGKKLKGEEAVALGI 180

Query: 181 VES-AHDGEDGVMKAAVRLGEQLGARNWNGEPYAEIRKSLHPEISGLLGL 230
           V+S AHD  +GV++A V LGE L A+ WNGE YA IRKSL+PE+  ++ L
Sbjct: 181 VDSAAHDSAEGVVEATVSLGESLAAKKWNGEVYATIRKSLYPELCRMVDL 228

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BLAST of Lsi05G017020 vs. TAIR10
Match: AT1G65520.1 (AT1G65520.1 delta(3), delta(2)-enoyl CoA isomerase 1)

HSP 1 Score: 206.1 bits (523), Expect = 2.5e-53
Identity = 109/224 (48.66%), Postives = 157/224 (70.09%), Query Frame = 1


Query: 1   MCTLEKRRNLFILTLTGDDDHRLSPSFIASILEALSKVKSQASRG-SVLITTSHGKFFSN 60
           MC+LEKR  LFIL LTGD +HRL+P+ + S+   +++++S  S   SVLITTS GKFFSN
Sbjct: 1   MCSLEKRDRLFILKLTGDGEHRLNPTLLDSLRSTINQIRSDPSFSQSVLITTSDGKFFSN 60

Query: 61  GFDLSWAQAAGSVSAAAERLRHMVEIFKPVVAQLLSLPMPTIAAIAGHAAAAGFMLALSH 120
           G+DL+ A++  S+S   +         + +VA L+SLPMPTIAA+ GHA+AAG +LA+SH
Sbjct: 61  GYDLALAESNPSLSVVMDAK------LRSLVADLISLPMPTIAAVTGHASAAGCILAMSH 120

Query: 121 DYLFMRSDRGVLYMSEVDLGLSLPDYFTALFKSKIGSSSVRRDVLLRGMKVKGEAAVRMG 180
           DY+ MR DRG LYMSE+D+ L +P +F A+ + KIGS + RRDV+L   KV  +  V+MG
Sbjct: 121 DYVLMRRDRGFLYMSELDIELIVPAWFMAVIRGKIGSPAARRDVMLTAAKVTADVGVKMG 180

Query: 181 IVESAHDGEDGVMKAAVRLGEQLGARNWNGEPYAEIRKSLHPEI 224
           IV+SA+      ++AA++LGE++  R  +G  Y ++R+SL  E+
Sbjct: 181 IVDSAYGSAAETVEAAIKLGEEIVQRGGDGHVYGKMRESLLREV 218

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BLAST of Lsi05G017020 vs. NCBI nr
Match: gi|449432586|ref|XP_004134080.1| (PREDICTED: 3-hydroxybutyryl-CoA dehydratase-like protein, mitochondrial [Cucumis sativus])

HSP 1 Score: 416.4 bits (1069), Expect = 3.4e-113
Identity = 215/239 (89.96%), Postives = 226/239 (94.56%), Query Frame = 1


Query: 1   MCTLEKRRNLFILTLTGDDDHRLSPSFIASILEALSKVKSQASRGSVLITTSHGKFFSNG 60
           MCTLEKRRNLFILTLTG DDHR SPS IA+ILEALSKVKSQAS GSVLITTSHGKFFSNG
Sbjct: 1   MCTLEKRRNLFILTLTGHDDHRFSPSVIAAILEALSKVKSQASTGSVLITTSHGKFFSNG 60

Query: 61  FDLSWAQAAGSVSAAAERLRHMVEIFKPVVAQLLSLPMPTIAAIAGHAAAAGFMLALSHD 120
           FDLSWAQAAGSVSAAAERL HMV+IFKPVVA+LLSLPMPTIAAI+GHAAAAGF+LALSHD
Sbjct: 61  FDLSWAQAAGSVSAAAERLHHMVQIFKPVVAELLSLPMPTIAAISGHAAAAGFLLALSHD 120

Query: 121 YLFMRSDRGVLYMSEVDLGLSLPDYFTALFKSKIGSSSVRRDVLLRGMKVKGEAAVRMGI 180
           YL MRSDRGVLYMSEVDLGLSLPDYF ALFKSKI SSSVRRDVL+RGMKVKGEAAV+MGI
Sbjct: 121 YLLMRSDRGVLYMSEVDLGLSLPDYFAALFKSKIASSSVRRDVLMRGMKVKGEAAVKMGI 180

Query: 181 VESAHDGEDGVMKAAVRLGEQLGARNWNGEPYAEIRKSLHPEISGLLGLPEKVISISKL 240
            +SAHDGE+GVM+AAVRLGEQLG R WNGEPYAEIRKSL+PEIS LLGLPEK ISISKL
Sbjct: 181 ADSAHDGENGVMEAAVRLGEQLGERKWNGEPYAEIRKSLYPEISRLLGLPEKAISISKL 239

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BLAST of Lsi05G017020 vs. NCBI nr
Match: gi|659076208|ref|XP_008438558.1| (PREDICTED: enoyl-CoA hydratase domain-containing protein 2, mitochondrial-like [Cucumis melo])

HSP 1 Score: 413.3 bits (1061), Expect = 2.9e-112
Identity = 213/239 (89.12%), Postives = 226/239 (94.56%), Query Frame = 1


Query: 1   MCTLEKRRNLFILTLTGDDDHRLSPSFIASILEALSKVKSQASRGSVLITTSHGKFFSNG 60
           MCTLEKRRNLFILTLTGD DHRL+PS IA++LEALSKVKSQAS GSVLITTSHGKFFSNG
Sbjct: 1   MCTLEKRRNLFILTLTGDHDHRLNPSVIAALLEALSKVKSQASPGSVLITTSHGKFFSNG 60

Query: 61  FDLSWAQAAGSVSAAAERLRHMVEIFKPVVAQLLSLPMPTIAAIAGHAAAAGFMLALSHD 120
           FDLSWA AAGSVSAAAERL HMV+ FKPV+A+LLSLPMPTIAAI+GHAAAAGFMLALSHD
Sbjct: 61  FDLSWAHAAGSVSAAAERLHHMVQNFKPVIAELLSLPMPTIAAISGHAAAAGFMLALSHD 120

Query: 121 YLFMRSDRGVLYMSEVDLGLSLPDYFTALFKSKIGSSSVRRDVLLRGMKVKGEAAVRMGI 180
           YL MRSDRGVLYMSEVDLGLSLPDYF ALFKSKIGSSSVRRDVL+RGMKVKGEAAV+MGI
Sbjct: 121 YLLMRSDRGVLYMSEVDLGLSLPDYFAALFKSKIGSSSVRRDVLMRGMKVKGEAAVKMGI 180

Query: 181 VESAHDGEDGVMKAAVRLGEQLGARNWNGEPYAEIRKSLHPEISGLLGLPEKVISISKL 240
            +SA+DGE GVM+AAVRLGEQLGAR WNGEPYAEIRKSL+PEIS LLGLPEK ISISKL
Sbjct: 181 ADSAYDGESGVMEAAVRLGEQLGARKWNGEPYAEIRKSLYPEISRLLGLPEKAISISKL 239

[Close]

BLAST of Lsi05G017020 vs. NCBI nr
Match: gi|449432584|ref|XP_004134079.1| (PREDICTED: 3-hydroxybutyryl-CoA dehydratase-like protein, mitochondrial [Cucumis sativus])

HSP 1 Score: 371.7 bits (953), Expect = 9.6e-100
Identity = 191/239 (79.92%), Postives = 211/239 (88.28%), Query Frame = 1


Query: 1   MCTLEKRRNLFILTLTGDDDHRLSPSFIASILEALSKVKSQASRGSVLITTSHGKFFSNG 60
           MCT+EKR N FILTLTGDDDHRLSPS I SIL+ALS+VK+QAS  SVLITTSHGKFFSNG
Sbjct: 1   MCTVEKRGNFFILTLTGDDDHRLSPSLINSILKALSQVKAQASPASVLITTSHGKFFSNG 60

Query: 61  FDLSWAQAAGSVSAAAERLRHMVEIFKPVVAQLLSLPMPTIAAIAGHAAAAGFMLALSHD 120
           FDL WA +AGSVSAA  RL H++EIFK VVA+L+SLPMPTIAAI GHAAAAGF LALSHD
Sbjct: 61  FDLPWALSAGSVSAARHRLNHLIEIFKSVVAELISLPMPTIAAITGHAAAAGFTLALSHD 120

Query: 121 YLFMRSDRGVLYMSEVDLGLSLPDYFTALFKSKIGSSSVRRDVLLRGMKVKGEAAVRMGI 180
           YL MRSDRGVLYM E+DLGL+LPDYF ALFKSKIGSS   RD++LRGMKV+GEAAVRMGI
Sbjct: 121 YLIMRSDRGVLYMRELDLGLTLPDYFMALFKSKIGSSLAMRDLILRGMKVRGEAAVRMGI 180

Query: 181 VESAHDGEDGVMKAAVRLGEQLGARNWNGEPYAEIRKSLHPEISGLLGLPEKVISISKL 240
           V+SAHDGED V+ AAVRLGEQL  RNW+GE YAEIRKSL+PE+S +LGLP K ISISKL
Sbjct: 181 VDSAHDGEDSVVNAAVRLGEQLAGRNWDGEVYAEIRKSLYPEVSEVLGLPGKPISISKL 239

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BLAST of Lsi05G017020 vs. NCBI nr
Match: gi|659076193|ref|XP_008438552.1| (PREDICTED: enoyl-CoA hydratase domain-containing protein 2, mitochondrial-like [Cucumis melo])

HSP 1 Score: 364.0 bits (933), Expect = 2.0e-97
Identity = 190/239 (79.50%), Postives = 208/239 (87.03%), Query Frame = 1


Query: 1   MCTLEKRRNLFILTLTGDDDHRLSPSFIASILEALSKVKSQASRGSVLITTSHGKFFSNG 60
           MCT+EKR N FILTLTGDDDHRLSPS I SIL+ALS+VK+QAS  SVLITTSHGKFFSNG
Sbjct: 1   MCTVEKRGNFFILTLTGDDDHRLSPSLINSILKALSQVKAQASPASVLITTSHGKFFSNG 60

Query: 61  FDLSWAQAAGSVSAAAERLRHMVEIFKPVVAQLLSLPMPTIAAIAGHAAAAGFMLALSHD 120
           FDL WA AAGSVSAA  RL H++EIFK VVA+L+SLPMPTIAAI GHAAAAGF LALSHD
Sbjct: 61  FDLPWALAAGSVSAARHRLNHIIEIFKSVVAELISLPMPTIAAITGHAAAAGFTLALSHD 120

Query: 121 YLFMRSDRGVLYMSEVDLGLSLPDYFTALFKSKIGSSSVRRDVLLRGMKVKGEAAVRMGI 180
           YL MRSDRGVLYM E+DLGL+LPDYF ALFKSKIGSS   RD++LRGMKV+GEAAVRMGI
Sbjct: 121 YLIMRSDRGVLYMRELDLGLTLPDYFMALFKSKIGSSLAMRDLILRGMKVRGEAAVRMGI 180

Query: 181 VESAHDGEDGVMKAAVRLGEQLGARNWNGEPYAEIRKSLHPEISGLLGLPEKVISISKL 240
           V+SAH  E  V+ AAVRLGEQL  RNW+GE YAEIRKSL+PEIS +LGLP K ISISKL
Sbjct: 181 VDSAHHSEVSVVNAAVRLGEQLVGRNWDGEVYAEIRKSLYPEISEVLGLPGKAISISKL 239

[Close]

BLAST of Lsi05G017020 vs. NCBI nr
Match: gi|449432588|ref|XP_004134081.1| (PREDICTED: 3-hydroxybutyryl-CoA dehydratase-like protein, mitochondrial [Cucumis sativus])

HSP 1 Score: 339.3 bits (869), Expect = 5.3e-90
Identity = 175/241 (72.61%), Postives = 205/241 (85.06%), Query Frame = 1


Query: 1   MCTLEKRRNLFILTLTGDDDHRLSPSFIASILEALSKVKSQ--ASRGSVLITTSHGKFFS 60
           MC++EKR  LFILTLTG+ +H L+PS I SIL ALS+VK++  AS GSVLITTS GKFFS
Sbjct: 1   MCSMEKRGKLFILTLTGNHEHWLNPSLINSILNALSQVKAEARASPGSVLITTSQGKFFS 60

Query: 61  NGFDLSWAQAAGSVSAAAERLRHMVEIFKPVVAQLLSLPMPTIAAIAGHAAAAGFMLALS 120
           NG DL W  +A S+SAA  RL HM+++FKP++A LL LP+PTIAA+ GHAAA G +LAL+
Sbjct: 61  NGLDLPWILSASSISAAHNRLNHMIQLFKPLLAHLLCLPIPTIAALPGHAAAGGLVLALA 120

Query: 121 HDYLFMRSDRGVLYMSEVDLGLSLPDYFTALFKSKIGSSSVRRDVLLRGMKVKGEAAVRM 180
           HDYL MRSDRGVLYMSE+DLG +LPDYF AL KSKIGSSSVRRDV LRGMKV+GE AV+M
Sbjct: 121 HDYLVMRSDRGVLYMSELDLGATLPDYFIALAKSKIGSSSVRRDVFLRGMKVRGETAVKM 180

Query: 181 GIVESAHDGEDGVMKAAVRLGEQLGARNWNGEPYAEIRKSLHPEISGLLGLPEKVISISK 240
           G+ ESAH GEDGVM+AAVRLGE+L ARNW+G+ YAEIRKSL+PEISGLLGL  KVI+ISK
Sbjct: 181 GLAESAHQGEDGVMEAAVRLGEELAARNWDGDVYAEIRKSLYPEISGLLGLTTKVITISK 240

The following BLAST results are available for this feature:

BLAST of Lsi05G017020 vs. Swiss-Prot


Match Name	E-value	Identity	Description
ECI2_ARATH	1.3e-85	66.25	Enoyl-CoA delta isomerase 2, peroxisomal OS=Arabidopsis thaliana GN=ECI2 PE=1 SV...	[more]
ECI3_ARATH	6.1e-78	63.91	Enoyl-CoA delta isomerase 3 OS=Arabidopsis thaliana GN=ECI3 PE=1 SV=1	[more]
ECI1_ARATH	4.4e-52	48.66	Enoyl-CoA delta isomerase 1, peroxisomal OS=Arabidopsis thaliana GN=ECI1 PE=1 SV...	[more]

BLAST of Lsi05G017020 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L8C7_CUCSA	2.4e-113	89.96	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G141780 PE=4 SV=1	[more]
A0A0A0L9X4_CUCSA	6.7e-100	79.92	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G141770 PE=4 SV=1	[more]
M5XMU9_PRUPE	3.8e-87	72.49	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa010715mg PE=4 SV=1	[more]
V4MKX3_EUTSA	1.1e-86	67.92	Uncharacterized protein OS=Eutrema salsugineum GN=EUTSA_v10027007mg PE=4 SV=1	[more]
A0A0D3DLS0_BRAOL	2.1e-85	67.50	Uncharacterized protein OS=Brassica oleracea var. oleracea GN=IBR10 PE=4 SV=1	[more]

BLAST of Lsi05G017020 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G14430.1	7.6e-87	66.25	indole-3-butyric acid response 10	[more]
AT4G14440.1	3.4e-79	63.91	3-hydroxyacyl-CoA dehydratase 1	[more]
AT1G65520.1	2.5e-53	48.66	delta(3), delta(2)-enoyl CoA isomerase 1	[more]

BLAST of Lsi05G017020 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449432586\|ref\|XP_004134080.1\|	3.4e-113	89.96	PREDICTED: 3-hydroxybutyryl-CoA dehydratase-like protein, mitochondrial [Cucumis...	[more]
gi\|659076208\|ref\|XP_008438558.1\|	2.9e-112	89.12	PREDICTED: enoyl-CoA hydratase domain-containing protein 2, mitochondrial-like [...	[more]
gi\|449432584\|ref\|XP_004134079.1\|	9.6e-100	79.92	PREDICTED: 3-hydroxybutyryl-CoA dehydratase-like protein, mitochondrial [Cucumis...	[more]
gi\|659076193\|ref\|XP_008438552.1\|	2.0e-97	79.50	PREDICTED: enoyl-CoA hydratase domain-containing protein 2, mitochondrial-like [...	[more]
gi\|449432588\|ref\|XP_004134081.1\|	5.3e-90	72.61	PREDICTED: 3-hydroxybutyryl-CoA dehydratase-like protein, mitochondrial [Cucumis...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Biological Process**
Term	Definition
GO:0008152	metabolic process

Vocabulary: **Molecular Function**
Term	Definition
GO:0003824	catalytic activity

Vocabulary: **INTERPRO**
Term	Definition
IPR001753	Enoyl-CoA_hydra/iso

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008152	metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0016853	isomerase activity
molecular_function	GO:0003824	catalytic activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi05G017020.1	Lsi05G017020.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001753	Crotonase superfamily	PFAM	PF00378	ECH_1	coord: 23..203 score: 5.5
None	No IPR available	PANTHER	PTHR11941	ENOYL-COA HYDRATASE-RELATED	coord: 1..223 score: 8.4
None	No IPR available	PANTHER	PTHR11941:SF84	SUBFAMILY NOT NAMED	coord: 1..223 score: 8.4

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi05G017020	BhiUN533G9	Wax gourd	lsiwgoB466

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi05G017020	Bottle gourd (USVL1VR-Ls)	lsilsiB011
Lsi05G017020	Cucumber (Gy14) v1	cgylsiB415
Lsi05G017020	Cucumber (Gy14) v1	cgylsiB620
Lsi05G017020	Cucurbita maxima (Rimu)	cmalsiB009
Lsi05G017020	Cucurbita maxima (Rimu)	cmalsiB245
Lsi05G017020	Cucurbita maxima (Rimu)	cmalsiB356
Lsi05G017020	Cucurbita maxima (Rimu)	cmalsiB586
Lsi05G017020	Cucurbita maxima (Rimu)	cmalsiB764
Lsi05G017020	Cucurbita maxima (Rimu)	cmalsiB839
Lsi05G017020	Cucurbita moschata (Rifu)	cmolsiB233
Lsi05G017020	Cucurbita moschata (Rifu)	cmolsiB345
Lsi05G017020	Cucurbita moschata (Rifu)	cmolsiB757
Lsi05G017020	Cucurbita moschata (Rifu)	cmolsiB823
Lsi05G017020	Cucurbita pepo (Zucchini)	cpelsiB267
Lsi05G017020	Cucurbita pepo (Zucchini)	cpelsiB506
Lsi05G017020	Cucurbita pepo (Zucchini)	cpelsiB617
Lsi05G017020	Cucurbita pepo (Zucchini)	cpelsiB714
Lsi05G017020	Wild cucumber (PI 183967)	cpilsiB125
Lsi05G017020	Wild cucumber (PI 183967)	cpilsiB207
Lsi05G017020	Cucumber (Chinese Long) v2	culsiB127
Lsi05G017020	Cucumber (Chinese Long) v2	culsiB209
Lsi05G017020	Melon (DHL92) v3.5.1	lsimeB360
Lsi05G017020	Melon (DHL92) v3.5.1	lsimeB378
Lsi05G017020	Watermelon (Charleston Gray)	lsiwcgB335
Lsi05G017020	Watermelon (Charleston Gray)	lsiwcgB343
Lsi05G017020	Watermelon (Charleston Gray)	lsiwcgB349
Lsi05G017020	Watermelon (97103) v1	lsiwmB359
Lsi05G017020	Watermelon (97103) v1	lsiwmB365
Lsi05G017020	Watermelon (97103) v1	lsiwmB382
Lsi05G017020	Cucumber (Gy14) v2	cgyblsiB115
Lsi05G017020	Cucumber (Gy14) v2	cgyblsiB196
Lsi05G017020	Melon (DHL92) v3.6.1	lsimedB375
Lsi05G017020	Melon (DHL92) v3.6.1	lsimedB396
Lsi05G017020	Silver-seed gourd	carlsiB206
Lsi05G017020	Silver-seed gourd	carlsiB210
Lsi05G017020	Silver-seed gourd	carlsiB718
Lsi05G017020	Silver-seed gourd	carlsiB878
Lsi05G017020	Silver-seed gourd	carlsiB983
Lsi05G017020	Cucumber (Chinese Long) v3	cuclsiB139
Lsi05G017020	Cucumber (Chinese Long) v3	cuclsiB228
Lsi05G017020	Watermelon (97103) v2	lsiwmbB333
Lsi05G017020	Watermelon (97103) v2	lsiwmbB341
Lsi05G017020	Watermelon (97103) v2	lsiwmbB347
Lsi05G017020	Wax gourd	lsiwgoB421
Lsi05G017020	Wax gourd	lsiwgoB462