Lsi05G007430.1 (mRNA) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi05G007430.1
Type	mRNA
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Cytochrome P450
Location	chr05 : 10293354 .. 10293935 (+)
Sequence length	582

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTAGAGAAGTAATGAAGACCCATGACCTCAAATTCTCCAATAGGCCCAAAACTACACCCACAAATCTCTTGTTCAACGGTTCCCAAGATGTGGGTTTTGCCCCATATGGTGAGTATTGGAGACGAGCCAAAAAAATGTGTGTTCTTGAGCTTTTTAGCGTGGAGTCTTTTCAATATGTTAGAGATGAAGAAGTTGATGTTCTTATCAATATGATTCGTAAGGTAGCTAATTGTTGTAGTGTTGACGGTGTTAATCTTAGCCAATTGTTCCTTAAAATCTCAACTGATATAGTGTCAAGATGTGTTATGGGAAAGAAATTTGAGGATGAAAATGAGAAGAGTAAATATGGAGATGTTTCAAGAAGGATAATGGAGCTATTGGCAGTGTTTTTTGTGGGGAATTTTTTCTCTGTTTTTGGATGGGTTGATCTAATTAGAGGGTTGGTTAGGAAAATTAAAATAGTTTCTAAGAAAATGGATGAATTCTTTGATTTGGTGATTGAAGACCACATCACAAAGTTGAAATCAAGTGGTAAATCTGATGAACATAAAAAGGATTTTGCGGATATTATGCTGTAA

mRNA sequence

ATGGCTAGAGAAGTAATGAAGACCCATGACCTCAAATTCTCCAATAGGCCCAAAACTACACCCACAAATCTCTTGTTCAACGGTTCCCAAGATGTGGGTTTTGCCCCATATGGTGAGTATTGGAGACGAGCCAAAAAAATGTGTGTTCTTGAGCTTTTTAGCGTGGAGTCTTTTCAATATGTTAGAGATGAAGAAGTTGATGTTCTTATCAATATGATTCGTAAGGTAGCTAATTGTTGTAGTGTTGACGGTGTTAATCTTAGCCAATTGTTCCTTAAAATCTCAACTGATATAGTGTCAAGATGTGTTATGGGAAAGAAATTTGAGGATGAAAATGAGAAGAGTAAATATGGAGATGTTTCAAGAAGGATAATGGAGCTATTGGCAGTGTTTTTTGTGGGGAATTTTTTCTCTGTTTTTGGATGGGTTGATCTAATTAGAGGGTTGGTTAGGAAAATTAAAATAGTTTCTAAGAAAATGGATGAATTCTTTGATTTGGTGATTGAAGACCACATCACAAAGTTGAAATCAAGTGGTAAATCTGATGAACATAAAAAGGATTTTGCGGATATTATGCTGTAA

Coding sequence (CDS)

ATGGCTAGAGAAGTAATGAAGACCCATGACCTCAAATTCTCCAATAGGCCCAAAACTACACCCACAAATCTCTTGTTCAACGGTTCCCAAGATGTGGGTTTTGCCCCATATGGTGAGTATTGGAGACGAGCCAAAAAAATGTGTGTTCTTGAGCTTTTTAGCGTGGAGTCTTTTCAATATGTTAGAGATGAAGAAGTTGATGTTCTTATCAATATGATTCGTAAGGTAGCTAATTGTTGTAGTGTTGACGGTGTTAATCTTAGCCAATTGTTCCTTAAAATCTCAACTGATATAGTGTCAAGATGTGTTATGGGAAAGAAATTTGAGGATGAAAATGAGAAGAGTAAATATGGAGATGTTTCAAGAAGGATAATGGAGCTATTGGCAGTGTTTTTTGTGGGGAATTTTTTCTCTGTTTTTGGATGGGTTGATCTAATTAGAGGGTTGGTTAGGAAAATTAAAATAGTTTCTAAGAAAATGGATGAATTCTTTGATTTGGTGATTGAAGACCACATCACAAAGTTGAAATCAAGTGGTAAATCTGATGAACATAAAAAGGATTTTGCGGATATTATGCTGTAA

Protein sequence

MAREVMKTHDLKFSNRPKTTPTNLLFNGSQDVGFAPYGEYWRRAKKMCVLELFSVESFQYVRDEEVDVLINMIRKVANCCSVDGVNLSQLFLKISTDIVSRCVMGKKFEDENEKSKYGDVSRRIMELLAVFFVGNFFSVFGWVDLIRGLVRKIKIVSKKMDEFFDLVIEDHITKLKSSGKSDEHKKDFADIML

Homology

[Close]

BLAST of Lsi05G007430.1 vs. Swiss-Prot
Match: C71A1_PERAE (Cytochrome P450 71A1 OS=Persea americana GN=CYP71A1 PE=1 SV=2)

HSP 1 Score: 167.9 bits (424), Expect = 1.1e-40
Identity = 80/197 (40.61%), Postives = 129/197 (65.48%), Query Frame = 1


Query: 1   MAREVMKTHDLKFSNRPKTTPTNLLFNGSQDVGFAPYGEYWRRAKKMCVLELFS---VES 60
           +A E++KTHDL F++RP TT    +F    DV F+PYGEYWR+ +K+CVLEL S   V S
Sbjct: 83  IAEEILKTHDLIFASRPSTTAARRIFYDCTDVAFSPYGEYWRQVRKICVLELLSIKRVNS 142

Query: 61  FQYVRDEEVDVLINMIRKVANCCSVDGVNLSQLFLKISTDIVSRCVMGKKFEDENE-KSK 120
           ++ +R+EEV +++  I +  +C + + VNLS+L L +S+  ++R   GKK+E E E K+K
Sbjct: 143 YRSIREEEVGLMMERISQ--SCSTGEAVNLSELLLLLSSGTITRVAFGKKYEGEEERKNK 202

Query: 121 YGDVSRRIMELLAVFFVGNFFSVFGWVDLIRGLVRKIKIVSKKMDEFFDLVIEDHITKLK 180
           + D++  +  L+  FFVG++F  F WVD++ G+  ++K    ++D F D VI+DH+   K
Sbjct: 203 FADLATELTTLMGAFFVGDYFPSFAWVDVLTGMDARLKRNHGELDAFVDHVIDDHLLSRK 262

Query: 181 SSGKSDEHKKDFADIML 194
           ++G     +KD  D++L
Sbjct: 263 ANGSDGVEQKDLVDVLL 277

[Close]

BLAST of Lsi05G007430.1 vs. Swiss-Prot
Match: C71AS_ARATH (Putative cytochrome P450 71A28 OS=Arabidopsis thaliana GN=CYP71A28 PE=3 SV=2)

HSP 1 Score: 155.6 bits (392), Expect = 5.5e-37
Identity = 81/196 (41.33%), Postives = 123/196 (62.76%), Query Frame = 1


Query: 1   MAREVMKTHDLKFSNRPKTTPTNLLFNGSQDVGFAPYGEYWRRAKKMCVLELFS---VES 60
           +A +VMKTHDLKF+NRPKT   +++ NG +DV F+ YGEYWR+ K +C + L     V S
Sbjct: 87  VAHDVMKTHDLKFANRPKTKAVDIIINGGRDVAFSSYGEYWRQMKSLCTVHLLGRQMVRS 146

Query: 61  FQYVRDEEVDVLINMIRKVANCCSVDGVNLSQLFLKISTDIVSRCVMGKKFEDENEKSKY 120
           F+ VR+EE+  +I  + K ++  S   VNLS L L +S D++ R  +G+K+  E   S +
Sbjct: 147 FEKVREEEITSVIWKLEKQSS--SSLPVNLSDLLLNMSNDVICRIAVGRKYSREENTSDF 206

Query: 121 GDVSRRIMELLAVFFVGNFFSVFGWVDLIRGLVRKIKIVSKKMDEFFDLVIEDHITKLKS 180
            +  R++MELL  F VG++     W+D +RGL RK++ VSK   EF + V+++H+     
Sbjct: 207 ENQLRKVMELLGAFPVGDYIPGLAWIDKVRGLDRKMEEVSKTFVEFLERVVQEHV----D 266

Query: 181 SGKSDEHKKDFADIML 194
            G++ E   DF DI+L
Sbjct: 267 EGENKE-TFDFVDILL 275

[Close]

BLAST of Lsi05G007430.1 vs. Swiss-Prot
Match: C71AD_ARATH (Indoleacetaldoxime dehydratase OS=Arabidopsis thaliana GN=CYP71A13 PE=1 SV=1)

HSP 1 Score: 154.5 bits (389), Expect = 1.2e-36
Identity = 78/195 (40.00%), Postives = 121/195 (62.05%), Query Frame = 1


Query: 2   AREVMKTHDLKFSNRPKTTPTNLLFNGSQDVGFAPYGEYWRRAKKMCVLELFS---VESF 61
           A+EV+KTHD KF+NRP++   + L NG +DV FAPYGEYWR+ K +C+L L +   VESF
Sbjct: 86  AQEVLKTHDHKFANRPRSKAVHGLMNGGRDVVFAPYGEYWRQMKSVCILNLLTNKMVESF 145

Query: 62  QYVRDEEVDVLINMIRKVANCCSVDGVNLSQLFLKISTDIVSRCVMGKKFEDENEKSKYG 121
           + VR++EV+ +I  + K ++  S +  NLS+LF+ + +D+ SR  +G+K  ++       
Sbjct: 146 EKVREDEVNAMIEKLEKASSSSSSE--NLSELFITLPSDVTSRVALGRKHSEDETARDLK 205

Query: 122 DVSRRIMELLAVFFVGNFFSVFGWVDLIRGLVRKIKIVSKKMDEFFDLVIEDHITKLKSS 181
              R+IMELL  F +G +  +  W+D IRG   KIK VS+   +  D V+++H+      
Sbjct: 206 KRVRQIMELLGEFPIGEYVPILAWIDGIRGFNNKIKEVSRGFSDLMDKVVQEHL------ 265

Query: 182 GKSDEHKKDFADIML 194
            ++   K DF DI+L
Sbjct: 266 -EASNDKADFVDILL 271

[Close]

BLAST of Lsi05G007430.1 vs. Swiss-Prot
Match: C71AI_ARATH (Cytochrome P450 71A18 OS=Arabidopsis thaliana GN=CYP71A18 PE=2 SV=2)

HSP 1 Score: 153.3 bits (386), Expect = 2.7e-36
Identity = 77/195 (39.49%), Postives = 120/195 (61.54%), Query Frame = 1


Query: 2   AREVMKTHDLKFSNRPKTTPTNLLFNGSQDVGFAPYGEYWRRAKKMCVLELFS---VESF 61
           A E++KTHDLKF+NRPK+   + L NG +DV F PYGEYWR+ K +C+L L +   V SF
Sbjct: 86  AHEILKTHDLKFANRPKSKAVHGLMNGGRDVVFGPYGEYWRQMKSVCILNLLTNKMVASF 145

Query: 62  QYVRDEEVDVLINMIRKVANCCSVDGVNLSQLFLKISTDIVSRCVMGKKFEDENEKSKYG 121
           + VR+EEV+ ++  + K +  CS    NLS+LF+ +++D+ SR  +GKK+ ++       
Sbjct: 146 EKVREEEVNAMMEKLEKAS--CSSSAENLSELFVTLTSDVTSRVSLGKKYWEDETAGGLK 205

Query: 122 DVSRRIMELLAVFFVGNFFSVFGWVDLIRGLVRKIKIVSKKMDEFFDLVIEDHITKLKSS 181
              R+IMELL  F +G++     W+D I G   KI  VS+   +  + V+++H+      
Sbjct: 206 KRVRQIMELLREFPIGDYVPALAWIDRINGFNSKIVEVSRAYSDLMEKVVQEHL------ 265

Query: 182 GKSDEHKKDFADIML 194
            ++ EHK DF +I+L
Sbjct: 266 -EAGEHKADFVNILL 271

[Close]

BLAST of Lsi05G007430.1 vs. Swiss-Prot
Match: C71AC_ARATH (Cytochrome P450 71A12 OS=Arabidopsis thaliana GN=CYP71A12 PE=2 SV=1)

HSP 1 Score: 151.8 bits (382), Expect = 7.9e-36
Identity = 75/195 (38.46%), Postives = 121/195 (62.05%), Query Frame = 1


Query: 2   AREVMKTHDLKFSNRPKTTPTNLLFNGSQDVGFAPYGEYWRRAKKMCVLELFS---VESF 61
           A+EV+KTHDLKF+NRP++   + L NG +DV F PYGEYWR+ K +C+L L +   V SF
Sbjct: 86  AQEVLKTHDLKFANRPRSKAVHGLMNGGRDVVFGPYGEYWRQMKSVCILNLLTNKMVASF 145

Query: 62  QYVRDEEVDVLINMIRKVANCCSVDGVNLSQLFLKISTDIVSRCVMGKKFEDENEKSKYG 121
           + +R+EE++ +I  + K ++  S +  NLS+LF+ + +D+ SR  +G+K  ++       
Sbjct: 146 EKIREEELNEMIKKLEKASSSSSSE--NLSELFVTLPSDVTSRIALGRKHSEDETARDLK 205

Query: 122 DVSRRIMELLAVFFVGNFFSVFGWVDLIRGLVRKIKIVSKKMDEFFDLVIEDHITKLKSS 181
              R+IMELL  F +G++     W+D I G   +IK VS+   +  D V+++H+      
Sbjct: 206 KRVRQIMELLGEFPIGDYVPALAWIDRINGFNARIKEVSQGFSDLMDKVVQEHL------ 265

Query: 182 GKSDEHKKDFADIML 194
            ++  HK+DF DI+L
Sbjct: 266 -EAGNHKEDFVDILL 271

[Close]

BLAST of Lsi05G007430.1 vs. TrEMBL
Match: W9RJY0_9ROSA (Cytochrome P450 71A1 OS=Morus notabilis GN=L484_018966 PE=3 SV=1)

HSP 1 Score: 182.2 bits (461), Expect = 6.1e-43
Identity = 86/197 (43.65%), Postives = 129/197 (65.48%), Query Frame = 1


Query: 1   MAREVMKTHDLKFSNRPKTTPTNLLFNGSQDVGFAPYGEYWRRAKKMCVLELFS---VES 60
           M +E++K HD+ FSNRPKTT  +++  GS DVGFAPYGEYWR+ +K+CVLEL S   V+ 
Sbjct: 111 MMKEIIKNHDITFSNRPKTTAADIILYGSHDVGFAPYGEYWRQTRKICVLELLSFRRVQQ 170

Query: 61  FQYVRDEEVDVLINMIRKVANCCSVDGVNLSQLFLKISTDIVSRCVMGKKFEDENEKSKY 120
           FQ+V++E    LI  IR+ +       VNLS++ +  S +I+SRCV+G+ FEDE+  SK+
Sbjct: 171 FQFVKEEVTVALIERIRRASQSSKDSSVNLSEMLIAASNNIMSRCVLGRDFEDEDGPSKF 230

Query: 121 GDVSRRIMELLAVFFVGNFFSVFGWVDLIRGLVRKIKIVSKKMDEFFDLVIEDHITKLKS 180
           G++ R++M  +  F VG+FF    WVD + G++ ++K +  ++D F D V+E+H   L+ 
Sbjct: 231 GELGRKLMVSMTTFSVGDFFPSLRWVDNLIGVIGRMKFIFSELDTFLDQVVEEHNAVLEG 290

Query: 181 SGK-SDEHKKDFADIML 194
                  + KDF DI L
Sbjct: 291 GEDIGGSNTKDFVDIFL 307

[Close]

BLAST of Lsi05G007430.1 vs. TrEMBL
Match: A0A061FYF5_THECC (Cytochrome P450 71A1 OS=Theobroma cacao GN=TCM_014496 PE=3 SV=1)

HSP 1 Score: 176.4 bits (446), Expect = 3.3e-41
Identity = 90/199 (45.23%), Postives = 130/199 (65.33%), Query Frame = 1


Query: 1   MAREVMKTHDLKFSNRPKTTPTNLLFNGSQDVGFAPYGEYWRRAKKMCVLELFS---VES 60
           MARE++K HD+ FSNRP+TT  N+L  G  D+GFAPYGEYWR+ +K  VLELFS   V S
Sbjct: 110 MAREIVKNHDIVFSNRPRTTAANILLYGCGDIGFAPYGEYWRQVRKTSVLELFSLRRVHS 169

Query: 61  FQYVRDEEVDVLINMIRKVANCCSVDGVNLSQLFLKISTDIVSRCVMGKKFEDENEKSKY 120
           FQ+VRDEEV++LIN I + +       +NL+++ + +S +IVSRCV+ ++ E+E+  +K+
Sbjct: 170 FQFVRDEEVELLINKIHRAS--LKGQSINLTEMLMTVSNNIVSRCVLSRRAEEEDGSNKF 229

Query: 121 GDVSRRIMELLAVFFVGNFFSVFGWVDLIRGLVRKIKIVSKKMDEFFDLVIEDHITKLKS 180
           G ++RRIM LL  F VG+ F    W+D++ G +  +K    ++D F   VIE+H      
Sbjct: 230 GQLARRIMVLLTSFCVGDMFPYLRWLDVLTGFIPSLKAAFGEIDAFLGQVIEEH-----R 289

Query: 181 SGKSDEH---KKDFADIML 194
             KS +H   K DF  I+L
Sbjct: 290 GLKSHDHMTDKMDFVSIIL 301

[Close]

BLAST of Lsi05G007430.1 vs. TrEMBL
Match: V4TQZ9_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10024390mg PE=3 SV=1)

HSP 1 Score: 176.0 bits (445), Expect = 4.4e-41
Identity = 80/185 (43.24%), Postives = 127/185 (68.65%), Query Frame = 1


Query: 1   MAREVMKTHDLKFSNRPKTTPTNLLFNGSQDVGFAPYGEYWRRAKKMCVLELFS---VES 60
           +A E++KTHD+  SNRPKTTP N+L    +DVGFA YGEYWR+ +K+CVL+L +   V+S
Sbjct: 93  LASEMIKTHDIVISNRPKTTPANILLYECRDVGFANYGEYWRKVRKICVLQLLNVRRVQS 152

Query: 61  FQYVRDEEVDVLINMIRKVANCCSVDGVNLSQLFLKISTDIVSRCVMGKKFEDENEKSKY 120
           FQ++RD+EV  L+N IR +  C +   +NL+++ L ++ +IVSRC +G++ E+EN KS +
Sbjct: 153 FQHIRDDEVSSLVNKIRNL--CLNGGSINLTEMLLAVTNNIVSRCALGRRVEEENGKSNF 212

Query: 121 GDVSRRIMELLAVFFVGNFFSVFGWVDLIRGLVRKIKIVSKKMDEFFDLVIEDHITKLKS 180
           G+VSR ++E L  F +G+ F   GW+D + GL  ++   ++ +++  D VIE+H+     
Sbjct: 213 GEVSRTLVEQLTAFCIGDVFPSLGWIDALSGLNGRLNATARALEDMLDQVIEEHMNNDMF 272

Query: 181 SGKSD 183
            G +D
Sbjct: 273 VGGTD 275

[Close]

BLAST of Lsi05G007430.1 vs. TrEMBL
Match: A0A0D2VQR5_GOSRA (Uncharacterized protein (Fragment) OS=Gossypium raimondii GN=B456_011G232800 PE=3 SV=1)

HSP 1 Score: 175.6 bits (444), Expect = 5.7e-41
Identity = 86/196 (43.88%), Postives = 131/196 (66.84%), Query Frame = 1


Query: 1   MAREVMKTHDLKFSNRPKTTPTNLLFNGSQDVGFAPYGEYWRRAKKMCVLELFS---VES 60
           + RE++K HD+ FSNRP+TT  ++LF G +D+ FAPYGEYWR+ KK+ V+ELFS   V S
Sbjct: 19  IVREIVKNHDIVFSNRPRTTAIDILFYGCKDMAFAPYGEYWRQVKKISVVELFSHRRVHS 78

Query: 61  FQYVRDEEVDVLINMIRKVANCCSVDGVNLSQLFLKISTDIVSRCVMGKKFEDENEKSKY 120
           FQ+VRDEEV++LIN IR  +       +NLS++   + ++IVSRCV+  K EDE+  SK+
Sbjct: 79  FQFVRDEEVELLINKIRSAS--LKGQSINLSEMLTSVFSNIVSRCVLSHKSEDEDGCSKF 138

Query: 121 GDVSRRIMELLAVFFVGNFFSVFGWVDLIRGLVRKIKIVSKKMDEFFDLVIEDHITKLKS 180
           G +++R+M L   F +G+ F    W+D++ G +  +K V+++ D FFD V+++H      
Sbjct: 139 GKLAKRLMILFTSFCIGDMFPYLRWLDVVTGYIPSLKAVNEEFDAFFDQVLQEHRALESD 198

Query: 181 SGKSDEHKKDFADIML 194
              SD  +KDF  I++
Sbjct: 199 DQVSD--RKDFVTIIM 210

[Close]

BLAST of Lsi05G007430.1 vs. TrEMBL
Match: V4T6H8_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10019648mg PE=3 SV=1)

HSP 1 Score: 175.6 bits (444), Expect = 5.7e-41
Identity = 92/204 (45.10%), Postives = 135/204 (66.18%), Query Frame = 1


Query: 1   MAREVMKTHDLKFSNRPKTTPTNLLFNGSQDVGFAPYGEYWRRAKKMCVLELFS---VES 60
           +A E+ KTHD+  SNRPKTTP N+L    QD+ F+ YGEYWR+A+K+C+L+L S   V+S
Sbjct: 108 LAGEMFKTHDIVISNRPKTTPANILVYECQDITFSNYGEYWRQARKICILQLLSVRRVQS 167

Query: 61  FQYVRDEEVDVLINMIRKVANCCSVDG-VNLSQLFLKISTDIVSRCVMGKKFEDENE--- 120
           FQYVRD+EV  L+  IR   +C +  G +NL+++ L  S++IVS CV+GKK ++E E   
Sbjct: 168 FQYVRDDEVSSLVTKIR--LSCLNKGGPLNLTEMLLTASSNIVSLCVLGKKADEEEENIG 227

Query: 121 -KSKYGDVSRRIMELLAVFFVGNFFSVFGWVDLIRGLVRKIKIVSKKMDEFFDLVIEDHI 180
             +K G++SRR+ E LA F +G+ F    W+D++ G + ++   ++  D   D VIE+H 
Sbjct: 228 DSNKLGELSRRLEEQLAAFCMGDMFPSLAWLDVLSGFIGRLNATARAFDALLDQVIEEHT 287

Query: 181 TKL-KSSGKSDEH--KKDFADIML 194
            K+ K SG  D+   KKDF DI+L
Sbjct: 288 NKVSKGSGHDDDQSDKKDFVDILL 309

[Close]

BLAST of Lsi05G007430.1 vs. TAIR10
Match: AT2G30770.1 (AT2G30770.1 cytochrome P450, family 71, subfamily A, polypeptide 13)

HSP 1 Score: 154.5 bits (389), Expect = 6.9e-38
Identity = 78/195 (40.00%), Postives = 121/195 (62.05%), Query Frame = 1


Query: 2   AREVMKTHDLKFSNRPKTTPTNLLFNGSQDVGFAPYGEYWRRAKKMCVLELFS---VESF 61
           A+EV+KTHD KF+NRP++   + L NG +DV FAPYGEYWR+ K +C+L L +   VESF
Sbjct: 92  AQEVLKTHDHKFANRPRSKAVHGLMNGGRDVVFAPYGEYWRQMKSVCILNLLTNKMVESF 151

Query: 62  QYVRDEEVDVLINMIRKVANCCSVDGVNLSQLFLKISTDIVSRCVMGKKFEDENEKSKYG 121
           + VR++EV+ +I  + K ++  S +  NLS+LF+ + +D+ SR  +G+K  ++       
Sbjct: 152 EKVREDEVNAMIEKLEKASSSSSSE--NLSELFITLPSDVTSRVALGRKHSEDETARDLK 211

Query: 122 DVSRRIMELLAVFFVGNFFSVFGWVDLIRGLVRKIKIVSKKMDEFFDLVIEDHITKLKSS 181
              R+IMELL  F +G +  +  W+D IRG   KIK VS+   +  D V+++H+      
Sbjct: 212 KRVRQIMELLGEFPIGEYVPILAWIDGIRGFNNKIKEVSRGFSDLMDKVVQEHL------ 271

Query: 182 GKSDEHKKDFADIML 194
            ++   K DF DI+L
Sbjct: 272 -EASNDKADFVDILL 277

[Close]

BLAST of Lsi05G007430.1 vs. TAIR10
Match: AT1G11610.2 (AT1G11610.2 cytochrome P450, family 71, subfamily A, polypeptide 18)

HSP 1 Score: 153.3 bits (386), Expect = 1.5e-37
Identity = 77/195 (39.49%), Postives = 120/195 (61.54%), Query Frame = 1


Query: 2   AREVMKTHDLKFSNRPKTTPTNLLFNGSQDVGFAPYGEYWRRAKKMCVLELFS---VESF 61
           A E++KTHDLKF+NRPK+   + L NG +DV F PYGEYWR+ K +C+L L +   V SF
Sbjct: 86  AHEILKTHDLKFANRPKSKAVHGLMNGGRDVVFGPYGEYWRQMKSVCILNLLTNKMVASF 145

Query: 62  QYVRDEEVDVLINMIRKVANCCSVDGVNLSQLFLKISTDIVSRCVMGKKFEDENEKSKYG 121
           + VR+EEV+ ++  + K +  CS    NLS+LF+ +++D+ SR  +GKK+ ++       
Sbjct: 146 EKVREEEVNAMMEKLEKAS--CSSSAENLSELFVTLTSDVTSRVSLGKKYWEDETAGGLK 205

Query: 122 DVSRRIMELLAVFFVGNFFSVFGWVDLIRGLVRKIKIVSKKMDEFFDLVIEDHITKLKSS 181
              R+IMELL  F +G++     W+D I G   KI  VS+   +  + V+++H+      
Sbjct: 206 KRVRQIMELLREFPIGDYVPALAWIDRINGFNSKIVEVSRAYSDLMEKVVQEHL------ 265

Query: 182 GKSDEHKKDFADIML 194
            ++ EHK DF +I+L
Sbjct: 266 -EAGEHKADFVNILL 271

[Close]

BLAST of Lsi05G007430.1 vs. TAIR10
Match: AT2G30750.1 (AT2G30750.1 cytochrome P450, family 71, subfamily A, polypeptide 12)

HSP 1 Score: 151.8 bits (382), Expect = 4.5e-37
Identity = 75/195 (38.46%), Postives = 121/195 (62.05%), Query Frame = 1


Query: 2   AREVMKTHDLKFSNRPKTTPTNLLFNGSQDVGFAPYGEYWRRAKKMCVLELFS---VESF 61
           A+EV+KTHDLKF+NRP++   + L NG +DV F PYGEYWR+ K +C+L L +   V SF
Sbjct: 92  AQEVLKTHDLKFANRPRSKAVHGLMNGGRDVVFGPYGEYWRQMKSVCILNLLTNKMVASF 151

Query: 62  QYVRDEEVDVLINMIRKVANCCSVDGVNLSQLFLKISTDIVSRCVMGKKFEDENEKSKYG 121
           + +R+EE++ +I  + K ++  S +  NLS+LF+ + +D+ SR  +G+K  ++       
Sbjct: 152 EKIREEELNEMIKKLEKASSSSSSE--NLSELFVTLPSDVTSRIALGRKHSEDETARDLK 211

Query: 122 DVSRRIMELLAVFFVGNFFSVFGWVDLIRGLVRKIKIVSKKMDEFFDLVIEDHITKLKSS 181
              R+IMELL  F +G++     W+D I G   +IK VS+   +  D V+++H+      
Sbjct: 212 KRVRQIMELLGEFPIGDYVPALAWIDRINGFNARIKEVSQGFSDLMDKVVQEHL------ 271

Query: 182 GKSDEHKKDFADIML 194
            ++  HK+DF DI+L
Sbjct: 272 -EAGNHKEDFVDILL 277

[Close]

BLAST of Lsi05G007430.1 vs. TAIR10
Match: AT4G13310.1 (AT4G13310.1 cytochrome P450, family 71, subfamily A, polypeptide 20)

HSP 1 Score: 143.7 bits (361), Expect = 1.2e-34
Identity = 66/174 (37.93%), Postives = 107/174 (61.49%), Query Frame = 1


Query: 1   MAREVMKTHDLKFSNRPKTTPTNLLFNGSQDVGFAPYGEYWRRAKKMCVLELFS---VES 60
           +A +VMKTHDL  +NRPKT   + + +G +DV FAPYGEYWR+ K +C+  L +   V S
Sbjct: 84  VAHDVMKTHDLVCANRPKTKVVDKILSGGRDVAFAPYGEYWRQMKSICIQNLLNNKMVRS 143

Query: 61  FQYVRDEEVDVLINMIRKVANCCSVDGVNLSQLFLKISTDIVSRCVMGKKFEDENEKSKY 120
           ++ +R+EE+  +I  + K +   S   VNLSQ+ + ++ DI+ R  +G+K+  + +    
Sbjct: 144 YEKIREEEIKRMIEKLEKASCSSSPSPVNLSQILMTLTNDIICRVALGRKYSGKKDGIDV 203

Query: 121 GDVSRRIMELLAVFFVGNFFSVFGWVDLIRGLVRKIKIVSKKMDEFFDLVIEDH 172
            ++ R    LL  F VG +     W+D IRGL  K+++V K+ DEF + V+++H
Sbjct: 204 ENIVRTFAALLGEFPVGEYIPSLSWIDRIRGLDHKMEVVDKRFDEFLERVVKEH 257

[Close]

BLAST of Lsi05G007430.1 vs. TAIR10
Match: AT3G48290.2 (AT3G48290.2 cytochrome P450, family 71, subfamily A, polypeptide 24)

HSP 1 Score: 141.7 bits (356), Expect = 4.6e-34
Identity = 73/195 (37.44%), Postives = 118/195 (60.51%), Query Frame = 1


Query: 2   AREVMKTHDLKFSNRPKTTPTNLLFNGSQDVGFAPYGEYWRRAKKMCVLELFS---VESF 61
           A++V+KTHD  F++RP++   + +F   +DV  APYGEYWR+ K +CVL LFS   V SF
Sbjct: 84  AKDVLKTHDRVFASRPRSKIFDKIFYNGRDVALAPYGEYWRQMKSVCVLHLFSNKMVRSF 143

Query: 62  QYVRDEEVDVLINMIRKVANCCSVDGVNLSQLFLKISTDIVSRCVMGKKFEDENEKSKYG 121
           + VR EE+ ++I  IR      S   +NLS++ + ++ +++ R  +G+K+     K+ + 
Sbjct: 144 RDVRQEEISLMIEKIR----ISSSLRINLSEILVNLTNNVICRVALGRKY---GGKTDFK 203

Query: 122 DVSRRIMELLAVFFVGNFFSVFGWVDLIRGLVRKIKIVSKKMDEFFDLVIEDHITKLKSS 181
           D+ +R+  LL  F VG++ S   W+D IRGL  ++  +S  +DEF + V++DH+      
Sbjct: 204 DLMKRLTRLLGEFSVGSYVSWLAWIDWIRGLDGQLIKISNDLDEFLERVVQDHV------ 263

Query: 182 GKSDEHKKDFADIML 194
              D HK DF D +L
Sbjct: 264 -DGDGHKNDFVDFLL 264

[Close]

BLAST of Lsi05G007430.1 vs. NCBI nr
Match: gi|659067363|ref|XP_008439061.1| (PREDICTED: cytochrome P450 71A1-like [Cucumis melo])

HSP 1 Score: 278.1 bits (710), Expect = 1.2e-71
Identity = 144/197 (73.10%), Postives = 166/197 (84.26%), Query Frame = 1


Query: 1   MAREVMKTHDLKFSNRPKTTPTNLLFNGSQDVGFAPYGEYWRRAKKMCVLELFS---VES 60
           +A E+MKTHDLKFSNRPKTT TNL+F G QDVGFAPYG+YWR+AKKMCVLELFS   VES
Sbjct: 18  VAGEIMKTHDLKFSNRPKTTATNLMFYGCQDVGFAPYGDYWRKAKKMCVLELFSHKRVES 77

Query: 61  FQYVRDEEVDVLINMIRKVANCCSVDGVNLSQLFLKISTDIVSRCVMGKKFEDENEKSKY 120
           FQYVRDEEVD+LIN IRK ANC   +G+NL+QL  +IS DIVSRCV+GKKFEDEN KS++
Sbjct: 78  FQYVRDEEVDILINKIRKRANCS--EGLNLTQLLFQISNDIVSRCVLGKKFEDENGKSEF 137

Query: 121 GDV-SRRIMELLAVFFVGNFFSVFGWVDLIRGLVRKIKIVSKKMDEFFDLVIEDHITKLK 180
            DV SRR+MELLA F VG+FF  FGWVD+IRG+V ++K VSK MD FFD+VIEDHI KLK
Sbjct: 138 RDVHSRRVMELLAAFCVGDFFPNFGWVDVIRGVVGEMKKVSKIMDGFFDMVIEDHIIKLK 197

Query: 181 SSGKSDEHKKDFADIML 194
           SS + D+ KKDF DIML
Sbjct: 198 SSDRCDD-KKDFVDIML 211

[Close]

BLAST of Lsi05G007430.1 vs. NCBI nr
Match: gi|778657943|ref|XP_004152573.2| (PREDICTED: cytochrome P450 71A1-like [Cucumis sativus])

HSP 1 Score: 260.8 bits (665), Expect = 1.9e-66
Identity = 134/197 (68.02%), Postives = 161/197 (81.73%), Query Frame = 1


Query: 1   MAREVMKTHDLKFSNRPKTTPTNLLFNGSQDVGFAPYGEYWRRAKKMCVLELFS---VES 60
           MARE+MKTHDL FSNRPKTT  N++F   QDVGF+PY + W++AKKMCV+ELFS   VES
Sbjct: 102 MAREIMKTHDLNFSNRPKTTAPNVMFYSCQDVGFSPYSDCWKKAKKMCVVELFSHKRVES 161

Query: 61  FQYVRDEEVDVLINMIRKVANCCSVDGVNLSQLFLKISTDIVSRCVMGKKFEDENEKSKY 120
           F+YVRDEEVD+LIN IRK ANC   +G+NL+QL  + S DIVSRCV+GKKFEDEN K+++
Sbjct: 162 FRYVRDEEVDILINKIRKGANCS--EGLNLTQLLFQTSNDIVSRCVLGKKFEDENGKNEF 221

Query: 121 GDV-SRRIMELLAVFFVGNFFSVFGWVDLIRGLVRKIKIVSKKMDEFFDLVIEDHITKLK 180
            DV SRR+MELL+ F VG+FF  FGWVD+IRGLV ++K VSK MD FFD+VIEDHI KLK
Sbjct: 222 RDVHSRRVMELLSAFCVGDFFPNFGWVDVIRGLVGEMKKVSKIMDGFFDMVIEDHIIKLK 281

Query: 181 SSGKSDEHKKDFADIML 194
           SS + D+ KKDF DIML
Sbjct: 282 SSDRCDD-KKDFVDIML 295

[Close]

BLAST of Lsi05G007430.1 vs. NCBI nr
Match: gi|778664798|ref|XP_011660355.1| (PREDICTED: cytochrome P450 71A1-like [Cucumis sativus])

HSP 1 Score: 211.5 bits (537), Expect = 1.3e-51
Identity = 111/202 (54.95%), Postives = 141/202 (69.80%), Query Frame = 1


Query: 1   MAREVMKTHDLKFSNRPKTTPTNLLFNGSQDVGFAPYGEYWRRAKKMCVLELFS---VES 60
           MA +VMKTHDL FSNRP+TT    L  G QD+GFAPYGEYWR+A+K+C LELFS   VES
Sbjct: 82  MAEQVMKTHDLVFSNRPQTTAAKTLLYGCQDMGFAPYGEYWRQARKICALELFSVKRVES 141

Query: 61  FQYVRDEEVDVLINMIRKVANCCSVDGVNLSQLFLKISTDIVSRCVMGKKFEDENEKSKY 120
           FQYVRDEE+D L+N IRK  +  S+D   L  LF + S +IVSRCVMG+KFEDE+ KS++
Sbjct: 142 FQYVRDEEIDSLVNKIRKSGSDGSLD---LGHLFFRTSNNIVSRCVMGEKFEDEDGKSRF 201

Query: 121 GDVSRRIMELLAVFFVGNFFSVFGW-VDLIRGLVRKIKIVSKKMDEFFDLVIEDHITKLK 180
            ++SR++M ++  F   +FF  FGW +D+IRG    +K   K +DEFF  V+E+H  K+K
Sbjct: 202 EEISRKVMVIITAFCFEDFFPSFGWIIDVIRGFHWDLKNCFKTLDEFFSKVVEEHKEKIK 261

Query: 181 SSG-----KSDEHKKDFADIML 194
            SG        E KKDF DIML
Sbjct: 262 RSGGVINIDDYESKKDFVDIML 280

[Close]

BLAST of Lsi05G007430.1 vs. NCBI nr
Match: gi|778657940|ref|XP_011651837.1| (PREDICTED: cytochrome P450 71A1-like [Cucumis sativus])

HSP 1 Score: 209.9 bits (533), Expect = 3.9e-51
Identity = 112/202 (55.45%), Postives = 142/202 (70.30%), Query Frame = 1


Query: 1   MAREVMKTHDLKFSNRPKTTPTNLLFNGSQDVGFAPYGEYWRRAKKMCVLELFS---VES 60
           +A +VMKTH L FSNRP+TT    L  G QDVGFAPYGEYWR+A+K+CVLELFS   VES
Sbjct: 82  IAEQVMKTHVLVFSNRPQTTAAKTLLYGCQDVGFAPYGEYWRQARKICVLELFSVKRVES 141

Query: 61  FQYVRDEEVDVLINMIRKVANCCSVDGVNLSQLFLKISTDIVSRCVMGKKFEDENEKSKY 120
           FQYVRDEE+D LIN IRK+ +  S+D   L  LF + S +IVSRCV+G+KFEDE+ KS++
Sbjct: 142 FQYVRDEEIDSLINKIRKIGSDQSLD---LGHLFFQTSNNIVSRCVLGEKFEDEDGKSRF 201

Query: 121 GDVSRRIMELLAVFFVGNFFSVFGW-VDLIRGLVRKIKIVSKKMDEFFDLVIEDHITKLK 180
            ++SR+ M ++  F V +FF  FGW +D+IRG    +K   K +DEFF  V+E+H  K+K
Sbjct: 202 EEISRKAMVIVTAFCVEDFFPSFGWIIDVIRGFHWDLKNCFKTLDEFFSKVVEEHKEKIK 261

Query: 181 SSG-----KSDEHKKDFADIML 194
            SG        E KKDF DIML
Sbjct: 262 RSGGVINIDDYESKKDFVDIML 280

[Close]

BLAST of Lsi05G007430.1 vs. NCBI nr
Match: gi|778657937|ref|XP_011651833.1| (PREDICTED: cytochrome P450 71A1-like [Cucumis sativus])

HSP 1 Score: 201.8 bits (512), Expect = 1.1e-48
Identity = 99/196 (50.51%), Postives = 136/196 (69.39%), Query Frame = 1


Query: 1   MAREVMKTHDLKFSNRPKTTPTNLLFNGSQDVGFAPYGEYWRRAKKMCVLELFS---VES 60
           MARE+ K HD+ FSNRPK+T  NL F G +DVGF+PYGEYWR  KK+C LEL S   V+ 
Sbjct: 83  MAREIFKNHDITFSNRPKSTAANLFFYGYKDVGFSPYGEYWRGLKKICTLELLSQRRVQG 142

Query: 61  FQYVRDEEVDVLINMIRKVANCCSVDGVNLSQLFLKISTDIVSRCVMGKKFEDENEKSKY 120
           FQYVR+EEV++L++ + K  +      VNLS L    S +I+SRC+ G+KFEDEN KS++
Sbjct: 143 FQYVREEEVEILVSKVHKATS--EGVSVNLSDLITSTSNNIISRCIFGEKFEDENGKSRF 202

Query: 121 GDVSRRIMELLAVFFVGNFFSVFGWVDLIRGLVRKIKIVSKKMDEFFDLVIEDHITKLKS 180
           G+++R++ +L+  F VG+FF  FGWVD I GL+ K+K  S  +D F +  I +H TK K 
Sbjct: 203 GELTRKMAKLVVGFSVGDFFPAFGWVDNITGLIGKLKETSGALDAFLEQFIAEHKTKKKD 262

Query: 181 SGKSDEHKKDFADIML 194
             +SD  ++DF DI+L
Sbjct: 263 DFRSD--REDFVDILL 274

The following BLAST results are available for this feature:

BLAST of Lsi05G007430.1 vs. Swiss-Prot


Match Name	E-value	Identity	Description
C71A1_PERAE	1.1e-40	40.61	Cytochrome P450 71A1 OS=Persea americana GN=CYP71A1 PE=1 SV=2	[more]
C71AS_ARATH	5.5e-37	41.33	Putative cytochrome P450 71A28 OS=Arabidopsis thaliana GN=CYP71A28 PE=3 SV=2	[more]
C71AD_ARATH	1.2e-36	40.00	Indoleacetaldoxime dehydratase OS=Arabidopsis thaliana GN=CYP71A13 PE=1 SV=1	[more]
C71AI_ARATH	2.7e-36	39.49	Cytochrome P450 71A18 OS=Arabidopsis thaliana GN=CYP71A18 PE=2 SV=2	[more]
C71AC_ARATH	7.9e-36	38.46	Cytochrome P450 71A12 OS=Arabidopsis thaliana GN=CYP71A12 PE=2 SV=1	[more]

BLAST of Lsi05G007430.1 vs. TrEMBL


Match Name	E-value	Identity	Description
W9RJY0_9ROSA	6.1e-43	43.65	Cytochrome P450 71A1 OS=Morus notabilis GN=L484_018966 PE=3 SV=1	[more]
A0A061FYF5_THECC	3.3e-41	45.23	Cytochrome P450 71A1 OS=Theobroma cacao GN=TCM_014496 PE=3 SV=1	[more]
V4TQZ9_9ROSI	4.4e-41	43.24	Uncharacterized protein OS=Citrus clementina GN=CICLE_v10024390mg PE=3 SV=1	[more]
A0A0D2VQR5_GOSRA	5.7e-41	43.88	Uncharacterized protein (Fragment) OS=Gossypium raimondii GN=B456_011G232800 PE=...	[more]
V4T6H8_9ROSI	5.7e-41	45.10	Uncharacterized protein OS=Citrus clementina GN=CICLE_v10019648mg PE=3 SV=1	[more]

BLAST of Lsi05G007430.1 vs. TAIR10


Match Name	E-value	Identity	Description
AT2G30770.1	6.9e-38	40.00	cytochrome P450, family 71, subfamily A, polypeptide 13	[more]
AT1G11610.2	1.5e-37	39.49	cytochrome P450, family 71, subfamily A, polypeptide 18	[more]
AT2G30750.1	4.5e-37	38.46	cytochrome P450, family 71, subfamily A, polypeptide 12	[more]
AT4G13310.1	1.2e-34	37.93	cytochrome P450, family 71, subfamily A, polypeptide 20	[more]
AT3G48290.2	4.6e-34	37.44	cytochrome P450, family 71, subfamily A, polypeptide 24	[more]

BLAST of Lsi05G007430.1 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659067363\|ref\|XP_008439061.1\|	1.2e-71	73.10	PREDICTED: cytochrome P450 71A1-like [Cucumis melo]	[more]
gi\|778657943\|ref\|XP_004152573.2\|	1.9e-66	68.02	PREDICTED: cytochrome P450 71A1-like [Cucumis sativus]	[more]
gi\|778664798\|ref\|XP_011660355.1\|	1.3e-51	54.95	PREDICTED: cytochrome P450 71A1-like [Cucumis sativus]	[more]
gi\|778657940\|ref\|XP_011651837.1\|	3.9e-51	55.45	PREDICTED: cytochrome P450 71A1-like [Cucumis sativus]	[more]
gi\|778657937\|ref\|XP_011651833.1\|	1.1e-48	50.51	PREDICTED: cytochrome P450 71A1-like [Cucumis sativus]	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **Biological Process**
Term	Definition
GO:0055114	oxidation-reduction process

Vocabulary: **Molecular Function**
Term	Definition
GO:0020037	heme binding
GO:0005506	iron ion binding
GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen

Vocabulary: **INTERPRO**
Term	Definition
IPR001128	Cyt_P450

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055114	oxidation-reduction process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Lsi05G007430	Lsi05G007430	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lsi05G007430.1.CDS.1	Lsi05G007430.1.CDS.1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Lsi05G007430.1	Lsi05G007430.1-protein	polypeptide

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	GENE3D	G3DSA:1.10.630.10		coord: 2..193 score: 7.3
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 1..193 score: 6.6
IPR001128	Cytochrome P450	unknown	SSF48264	Cytochrome P450	coord: 1..193 score: 9.3
None	No IPR available	PANTHER	PTHR24298	FAMILY NOT NAMED	coord: 1..193 score: 2.1
None	No IPR available	PANTHER	PTHR24298:SF86	CYTOCHROME P450 71B21-RELATED	coord: 1..193 score: 2.1