Lsi05G007430 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi05G007430
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Cytochrome P450
Location	chr05 : 10293354 .. 10293935 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTAGAGAAGTAATGAAGACCCATGACCTCAAATTCTCCAATAGGCCCAAAACTACACCCACAAATCTCTTGTTCAACGGTTCCCAAGATGTGGGTTTTGCCCCATATGGTGAGTATTGGAGACGAGCCAAAAAAATGTGTGTTCTTGAGCTTTTTAGCGTGGAGTCTTTTCAATATGTTAGAGATGAAGAAGTTGATGTTCTTATCAATATGATTCGTAAGGTAGCTAATTGTTGTAGTGTTGACGGTGTTAATCTTAGCCAATTGTTCCTTAAAATCTCAACTGATATAGTGTCAAGATGTGTTATGGGAAAGAAATTTGAGGATGAAAATGAGAAGAGTAAATATGGAGATGTTTCAAGAAGGATAATGGAGCTATTGGCAGTGTTTTTTGTGGGGAATTTTTTCTCTGTTTTTGGATGGGTTGATCTAATTAGAGGGTTGGTTAGGAAAATTAAAATAGTTTCTAAGAAAATGGATGAATTCTTTGATTTGGTGATTGAAGACCACATCACAAAGTTGAAATCAAGTGGTAAATCTGATGAACATAAAAAGGATTTTGCGGATATTATGCTGTAA

mRNA sequence

ATGGCTAGAGAAGTAATGAAGACCCATGACCTCAAATTCTCCAATAGGCCCAAAACTACACCCACAAATCTCTTGTTCAACGGTTCCCAAGATGTGGGTTTTGCCCCATATGGTGAGTATTGGAGACGAGCCAAAAAAATGTGTGTTCTTGAGCTTTTTAGCGTGGAGTCTTTTCAATATGTTAGAGATGAAGAAGTTGATGTTCTTATCAATATGATTCGTAAGGTAGCTAATTGTTGTAGTGTTGACGGTGTTAATCTTAGCCAATTGTTCCTTAAAATCTCAACTGATATAGTGTCAAGATGTGTTATGGGAAAGAAATTTGAGGATGAAAATGAGAAGAGTAAATATGGAGATGTTTCAAGAAGGATAATGGAGCTATTGGCAGTGTTTTTTGTGGGGAATTTTTTCTCTGTTTTTGGATGGGTTGATCTAATTAGAGGGTTGGTTAGGAAAATTAAAATAGTTTCTAAGAAAATGGATGAATTCTTTGATTTGGTGATTGAAGACCACATCACAAAGTTGAAATCAAGTGGTAAATCTGATGAACATAAAAAGGATTTTGCGGATATTATGCTGTAA

Coding sequence (CDS)

ATGGCTAGAGAAGTAATGAAGACCCATGACCTCAAATTCTCCAATAGGCCCAAAACTACACCCACAAATCTCTTGTTCAACGGTTCCCAAGATGTGGGTTTTGCCCCATATGGTGAGTATTGGAGACGAGCCAAAAAAATGTGTGTTCTTGAGCTTTTTAGCGTGGAGTCTTTTCAATATGTTAGAGATGAAGAAGTTGATGTTCTTATCAATATGATTCGTAAGGTAGCTAATTGTTGTAGTGTTGACGGTGTTAATCTTAGCCAATTGTTCCTTAAAATCTCAACTGATATAGTGTCAAGATGTGTTATGGGAAAGAAATTTGAGGATGAAAATGAGAAGAGTAAATATGGAGATGTTTCAAGAAGGATAATGGAGCTATTGGCAGTGTTTTTTGTGGGGAATTTTTTCTCTGTTTTTGGATGGGTTGATCTAATTAGAGGGTTGGTTAGGAAAATTAAAATAGTTTCTAAGAAAATGGATGAATTCTTTGATTTGGTGATTGAAGACCACATCACAAAGTTGAAATCAAGTGGTAAATCTGATGAACATAAAAAGGATTTTGCGGATATTATGCTGTAA

Protein sequence

MAREVMKTHDLKFSNRPKTTPTNLLFNGSQDVGFAPYGEYWRRAKKMCVLELFSVESFQYVRDEEVDVLINMIRKVANCCSVDGVNLSQLFLKISTDIVSRCVMGKKFEDENEKSKYGDVSRRIMELLAVFFVGNFFSVFGWVDLIRGLVRKIKIVSKKMDEFFDLVIEDHITKLKSSGKSDEHKKDFADIML

Homology

[Close]

BLAST of Lsi05G007430 vs. Swiss-Prot
Match: C71A1_PERAE (Cytochrome P450 71A1 OS=Persea americana GN=CYP71A1 PE=1 SV=2)

HSP 1 Score: 167.9 bits (424), Expect = 1.1e-40
Identity = 80/197 (40.61%), Postives = 129/197 (65.48%), Query Frame = 1


Query: 1   MAREVMKTHDLKFSNRPKTTPTNLLFNGSQDVGFAPYGEYWRRAKKMCVLELFS---VES 60
           +A E++KTHDL F++RP TT    +F    DV F+PYGEYWR+ +K+CVLEL S   V S
Sbjct: 83  IAEEILKTHDLIFASRPSTTAARRIFYDCTDVAFSPYGEYWRQVRKICVLELLSIKRVNS 142

Query: 61  FQYVRDEEVDVLINMIRKVANCCSVDGVNLSQLFLKISTDIVSRCVMGKKFEDENE-KSK 120
           ++ +R+EEV +++  I +  +C + + VNLS+L L +S+  ++R   GKK+E E E K+K
Sbjct: 143 YRSIREEEVGLMMERISQ--SCSTGEAVNLSELLLLLSSGTITRVAFGKKYEGEEERKNK 202

Query: 121 YGDVSRRIMELLAVFFVGNFFSVFGWVDLIRGLVRKIKIVSKKMDEFFDLVIEDHITKLK 180
           + D++  +  L+  FFVG++F  F WVD++ G+  ++K    ++D F D VI+DH+   K
Sbjct: 203 FADLATELTTLMGAFFVGDYFPSFAWVDVLTGMDARLKRNHGELDAFVDHVIDDHLLSRK 262

Query: 181 SSGKSDEHKKDFADIML 194
           ++G     +KD  D++L
Sbjct: 263 ANGSDGVEQKDLVDVLL 277

[Close]

BLAST of Lsi05G007430 vs. Swiss-Prot
Match: C71AS_ARATH (Putative cytochrome P450 71A28 OS=Arabidopsis thaliana GN=CYP71A28 PE=3 SV=2)

HSP 1 Score: 155.6 bits (392), Expect = 5.5e-37
Identity = 81/196 (41.33%), Postives = 123/196 (62.76%), Query Frame = 1


Query: 1   MAREVMKTHDLKFSNRPKTTPTNLLFNGSQDVGFAPYGEYWRRAKKMCVLELFS---VES 60
           +A +VMKTHDLKF+NRPKT   +++ NG +DV F+ YGEYWR+ K +C + L     V S
Sbjct: 87  VAHDVMKTHDLKFANRPKTKAVDIIINGGRDVAFSSYGEYWRQMKSLCTVHLLGRQMVRS 146

Query: 61  FQYVRDEEVDVLINMIRKVANCCSVDGVNLSQLFLKISTDIVSRCVMGKKFEDENEKSKY 120
           F+ VR+EE+  +I  + K ++  S   VNLS L L +S D++ R  +G+K+  E   S +
Sbjct: 147 FEKVREEEITSVIWKLEKQSS--SSLPVNLSDLLLNMSNDVICRIAVGRKYSREENTSDF 206

Query: 121 GDVSRRIMELLAVFFVGNFFSVFGWVDLIRGLVRKIKIVSKKMDEFFDLVIEDHITKLKS 180
            +  R++MELL  F VG++     W+D +RGL RK++ VSK   EF + V+++H+     
Sbjct: 207 ENQLRKVMELLGAFPVGDYIPGLAWIDKVRGLDRKMEEVSKTFVEFLERVVQEHV----D 266

Query: 181 SGKSDEHKKDFADIML 194
            G++ E   DF DI+L
Sbjct: 267 EGENKE-TFDFVDILL 275

[Close]

BLAST of Lsi05G007430 vs. Swiss-Prot
Match: C71AD_ARATH (Indoleacetaldoxime dehydratase OS=Arabidopsis thaliana GN=CYP71A13 PE=1 SV=1)

HSP 1 Score: 154.5 bits (389), Expect = 1.2e-36
Identity = 78/195 (40.00%), Postives = 121/195 (62.05%), Query Frame = 1


Query: 2   AREVMKTHDLKFSNRPKTTPTNLLFNGSQDVGFAPYGEYWRRAKKMCVLELFS---VESF 61
           A+EV+KTHD KF+NRP++   + L NG +DV FAPYGEYWR+ K +C+L L +   VESF
Sbjct: 86  AQEVLKTHDHKFANRPRSKAVHGLMNGGRDVVFAPYGEYWRQMKSVCILNLLTNKMVESF 145

Query: 62  QYVRDEEVDVLINMIRKVANCCSVDGVNLSQLFLKISTDIVSRCVMGKKFEDENEKSKYG 121
           + VR++EV+ +I  + K ++  S +  NLS+LF+ + +D+ SR  +G+K  ++       
Sbjct: 146 EKVREDEVNAMIEKLEKASSSSSSE--NLSELFITLPSDVTSRVALGRKHSEDETARDLK 205

Query: 122 DVSRRIMELLAVFFVGNFFSVFGWVDLIRGLVRKIKIVSKKMDEFFDLVIEDHITKLKSS 181
              R+IMELL  F +G +  +  W+D IRG   KIK VS+   +  D V+++H+      
Sbjct: 206 KRVRQIMELLGEFPIGEYVPILAWIDGIRGFNNKIKEVSRGFSDLMDKVVQEHL------ 265

Query: 182 GKSDEHKKDFADIML 194
            ++   K DF DI+L
Sbjct: 266 -EASNDKADFVDILL 271

[Close]

BLAST of Lsi05G007430 vs. Swiss-Prot
Match: C71AI_ARATH (Cytochrome P450 71A18 OS=Arabidopsis thaliana GN=CYP71A18 PE=2 SV=2)

HSP 1 Score: 153.3 bits (386), Expect = 2.7e-36
Identity = 77/195 (39.49%), Postives = 120/195 (61.54%), Query Frame = 1


Query: 2   AREVMKTHDLKFSNRPKTTPTNLLFNGSQDVGFAPYGEYWRRAKKMCVLELFS---VESF 61
           A E++KTHDLKF+NRPK+   + L NG +DV F PYGEYWR+ K +C+L L +   V SF
Sbjct: 86  AHEILKTHDLKFANRPKSKAVHGLMNGGRDVVFGPYGEYWRQMKSVCILNLLTNKMVASF 145

Query: 62  QYVRDEEVDVLINMIRKVANCCSVDGVNLSQLFLKISTDIVSRCVMGKKFEDENEKSKYG 121
           + VR+EEV+ ++  + K +  CS    NLS+LF+ +++D+ SR  +GKK+ ++       
Sbjct: 146 EKVREEEVNAMMEKLEKAS--CSSSAENLSELFVTLTSDVTSRVSLGKKYWEDETAGGLK 205

Query: 122 DVSRRIMELLAVFFVGNFFSVFGWVDLIRGLVRKIKIVSKKMDEFFDLVIEDHITKLKSS 181
              R+IMELL  F +G++     W+D I G   KI  VS+   +  + V+++H+      
Sbjct: 206 KRVRQIMELLREFPIGDYVPALAWIDRINGFNSKIVEVSRAYSDLMEKVVQEHL------ 265

Query: 182 GKSDEHKKDFADIML 194
            ++ EHK DF +I+L
Sbjct: 266 -EAGEHKADFVNILL 271

[Close]

BLAST of Lsi05G007430 vs. Swiss-Prot
Match: C71AC_ARATH (Cytochrome P450 71A12 OS=Arabidopsis thaliana GN=CYP71A12 PE=2 SV=1)

HSP 1 Score: 151.8 bits (382), Expect = 7.9e-36
Identity = 75/195 (38.46%), Postives = 121/195 (62.05%), Query Frame = 1


Query: 2   AREVMKTHDLKFSNRPKTTPTNLLFNGSQDVGFAPYGEYWRRAKKMCVLELFS---VESF 61
           A+EV+KTHDLKF+NRP++   + L NG +DV F PYGEYWR+ K +C+L L +   V SF
Sbjct: 86  AQEVLKTHDLKFANRPRSKAVHGLMNGGRDVVFGPYGEYWRQMKSVCILNLLTNKMVASF 145

Query: 62  QYVRDEEVDVLINMIRKVANCCSVDGVNLSQLFLKISTDIVSRCVMGKKFEDENEKSKYG 121
           + +R+EE++ +I  + K ++  S +  NLS+LF+ + +D+ SR  +G+K  ++       
Sbjct: 146 EKIREEELNEMIKKLEKASSSSSSE--NLSELFVTLPSDVTSRIALGRKHSEDETARDLK 205

Query: 122 DVSRRIMELLAVFFVGNFFSVFGWVDLIRGLVRKIKIVSKKMDEFFDLVIEDHITKLKSS 181
              R+IMELL  F +G++     W+D I G   +IK VS+   +  D V+++H+      
Sbjct: 206 KRVRQIMELLGEFPIGDYVPALAWIDRINGFNARIKEVSQGFSDLMDKVVQEHL------ 265

Query: 182 GKSDEHKKDFADIML 194
            ++  HK+DF DI+L
Sbjct: 266 -EAGNHKEDFVDILL 271

[Close]

BLAST of Lsi05G007430 vs. TrEMBL
Match: W9RJY0_9ROSA (Cytochrome P450 71A1 OS=Morus notabilis GN=L484_018966 PE=3 SV=1)

HSP 1 Score: 182.2 bits (461), Expect = 6.1e-43
Identity = 86/197 (43.65%), Postives = 129/197 (65.48%), Query Frame = 1


Query: 1   MAREVMKTHDLKFSNRPKTTPTNLLFNGSQDVGFAPYGEYWRRAKKMCVLELFS---VES 60
           M +E++K HD+ FSNRPKTT  +++  GS DVGFAPYGEYWR+ +K+CVLEL S   V+ 
Sbjct: 111 MMKEIIKNHDITFSNRPKTTAADIILYGSHDVGFAPYGEYWRQTRKICVLELLSFRRVQQ 170

Query: 61  FQYVRDEEVDVLINMIRKVANCCSVDGVNLSQLFLKISTDIVSRCVMGKKFEDENEKSKY 120
           FQ+V++E    LI  IR+ +       VNLS++ +  S +I+SRCV+G+ FEDE+  SK+
Sbjct: 171 FQFVKEEVTVALIERIRRASQSSKDSSVNLSEMLIAASNNIMSRCVLGRDFEDEDGPSKF 230

Query: 121 GDVSRRIMELLAVFFVGNFFSVFGWVDLIRGLVRKIKIVSKKMDEFFDLVIEDHITKLKS 180
           G++ R++M  +  F VG+FF    WVD + G++ ++K +  ++D F D V+E+H   L+ 
Sbjct: 231 GELGRKLMVSMTTFSVGDFFPSLRWVDNLIGVIGRMKFIFSELDTFLDQVVEEHNAVLEG 290

Query: 181 SGK-SDEHKKDFADIML 194
                  + KDF DI L
Sbjct: 291 GEDIGGSNTKDFVDIFL 307

[Close]

BLAST of Lsi05G007430 vs. TrEMBL
Match: A0A061FYF5_THECC (Cytochrome P450 71A1 OS=Theobroma cacao GN=TCM_014496 PE=3 SV=1)

HSP 1 Score: 176.4 bits (446), Expect = 3.3e-41
Identity = 90/199 (45.23%), Postives = 130/199 (65.33%), Query Frame = 1


Query: 1   MAREVMKTHDLKFSNRPKTTPTNLLFNGSQDVGFAPYGEYWRRAKKMCVLELFS---VES 60
           MARE++K HD+ FSNRP+TT  N+L  G  D+GFAPYGEYWR+ +K  VLELFS   V S
Sbjct: 110 MAREIVKNHDIVFSNRPRTTAANILLYGCGDIGFAPYGEYWRQVRKTSVLELFSLRRVHS 169

Query: 61  FQYVRDEEVDVLINMIRKVANCCSVDGVNLSQLFLKISTDIVSRCVMGKKFEDENEKSKY 120
           FQ+VRDEEV++LIN I + +       +NL+++ + +S +IVSRCV+ ++ E+E+  +K+
Sbjct: 170 FQFVRDEEVELLINKIHRAS--LKGQSINLTEMLMTVSNNIVSRCVLSRRAEEEDGSNKF 229

Query: 121 GDVSRRIMELLAVFFVGNFFSVFGWVDLIRGLVRKIKIVSKKMDEFFDLVIEDHITKLKS 180
           G ++RRIM LL  F VG+ F    W+D++ G +  +K    ++D F   VIE+H      
Sbjct: 230 GQLARRIMVLLTSFCVGDMFPYLRWLDVLTGFIPSLKAAFGEIDAFLGQVIEEH-----R 289

Query: 181 SGKSDEH---KKDFADIML 194
             KS +H   K DF  I+L
Sbjct: 290 GLKSHDHMTDKMDFVSIIL 301

[Close]

BLAST of Lsi05G007430 vs. TrEMBL
Match: V4TQZ9_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10024390mg PE=3 SV=1)

HSP 1 Score: 176.0 bits (445), Expect = 4.4e-41
Identity = 80/185 (43.24%), Postives = 127/185 (68.65%), Query Frame = 1


Query: 1   MAREVMKTHDLKFSNRPKTTPTNLLFNGSQDVGFAPYGEYWRRAKKMCVLELFS---VES 60
           +A E++KTHD+  SNRPKTTP N+L    +DVGFA YGEYWR+ +K+CVL+L +   V+S
Sbjct: 93  LASEMIKTHDIVISNRPKTTPANILLYECRDVGFANYGEYWRKVRKICVLQLLNVRRVQS 152

Query: 61  FQYVRDEEVDVLINMIRKVANCCSVDGVNLSQLFLKISTDIVSRCVMGKKFEDENEKSKY 120
           FQ++RD+EV  L+N IR +  C +   +NL+++ L ++ +IVSRC +G++ E+EN KS +
Sbjct: 153 FQHIRDDEVSSLVNKIRNL--CLNGGSINLTEMLLAVTNNIVSRCALGRRVEEENGKSNF 212

Query: 121 GDVSRRIMELLAVFFVGNFFSVFGWVDLIRGLVRKIKIVSKKMDEFFDLVIEDHITKLKS 180
           G+VSR ++E L  F +G+ F   GW+D + GL  ++   ++ +++  D VIE+H+     
Sbjct: 213 GEVSRTLVEQLTAFCIGDVFPSLGWIDALSGLNGRLNATARALEDMLDQVIEEHMNNDMF 272

Query: 181 SGKSD 183
            G +D
Sbjct: 273 VGGTD 275

[Close]

BLAST of Lsi05G007430 vs. TrEMBL
Match: A0A0D2VQR5_GOSRA (Uncharacterized protein (Fragment) OS=Gossypium raimondii GN=B456_011G232800 PE=3 SV=1)

HSP 1 Score: 175.6 bits (444), Expect = 5.7e-41
Identity = 86/196 (43.88%), Postives = 131/196 (66.84%), Query Frame = 1


Query: 1   MAREVMKTHDLKFSNRPKTTPTNLLFNGSQDVGFAPYGEYWRRAKKMCVLELFS---VES 60
           + RE++K HD+ FSNRP+TT  ++LF G +D+ FAPYGEYWR+ KK+ V+ELFS   V S
Sbjct: 19  IVREIVKNHDIVFSNRPRTTAIDILFYGCKDMAFAPYGEYWRQVKKISVVELFSHRRVHS 78

Query: 61  FQYVRDEEVDVLINMIRKVANCCSVDGVNLSQLFLKISTDIVSRCVMGKKFEDENEKSKY 120
           FQ+VRDEEV++LIN IR  +       +NLS++   + ++IVSRCV+  K EDE+  SK+
Sbjct: 79  FQFVRDEEVELLINKIRSAS--LKGQSINLSEMLTSVFSNIVSRCVLSHKSEDEDGCSKF 138

Query: 121 GDVSRRIMELLAVFFVGNFFSVFGWVDLIRGLVRKIKIVSKKMDEFFDLVIEDHITKLKS 180
           G +++R+M L   F +G+ F    W+D++ G +  +K V+++ D FFD V+++H      
Sbjct: 139 GKLAKRLMILFTSFCIGDMFPYLRWLDVVTGYIPSLKAVNEEFDAFFDQVLQEHRALESD 198

Query: 181 SGKSDEHKKDFADIML 194
              SD  +KDF  I++
Sbjct: 199 DQVSD--RKDFVTIIM 210

[Close]

BLAST of Lsi05G007430 vs. TrEMBL
Match: V4T6H8_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10019648mg PE=3 SV=1)

HSP 1 Score: 175.6 bits (444), Expect = 5.7e-41
Identity = 92/204 (45.10%), Postives = 135/204 (66.18%), Query Frame = 1


Query: 1   MAREVMKTHDLKFSNRPKTTPTNLLFNGSQDVGFAPYGEYWRRAKKMCVLELFS---VES 60
           +A E+ KTHD+  SNRPKTTP N+L    QD+ F+ YGEYWR+A+K+C+L+L S   V+S
Sbjct: 108 LAGEMFKTHDIVISNRPKTTPANILVYECQDITFSNYGEYWRQARKICILQLLSVRRVQS 167

Query: 61  FQYVRDEEVDVLINMIRKVANCCSVDG-VNLSQLFLKISTDIVSRCVMGKKFEDENE--- 120
           FQYVRD+EV  L+  IR   +C +  G +NL+++ L  S++IVS CV+GKK ++E E   
Sbjct: 168 FQYVRDDEVSSLVTKIR--LSCLNKGGPLNLTEMLLTASSNIVSLCVLGKKADEEEENIG 227

Query: 121 -KSKYGDVSRRIMELLAVFFVGNFFSVFGWVDLIRGLVRKIKIVSKKMDEFFDLVIEDHI 180
             +K G++SRR+ E LA F +G+ F    W+D++ G + ++   ++  D   D VIE+H 
Sbjct: 228 DSNKLGELSRRLEEQLAAFCMGDMFPSLAWLDVLSGFIGRLNATARAFDALLDQVIEEHT 287

Query: 181 TKL-KSSGKSDEH--KKDFADIML 194
            K+ K SG  D+   KKDF DI+L
Sbjct: 288 NKVSKGSGHDDDQSDKKDFVDILL 309

[Close]

BLAST of Lsi05G007430 vs. TAIR10
Match: AT2G30770.1 (AT2G30770.1 cytochrome P450, family 71, subfamily A, polypeptide 13)

HSP 1 Score: 154.5 bits (389), Expect = 6.9e-38
Identity = 78/195 (40.00%), Postives = 121/195 (62.05%), Query Frame = 1


Query: 2   AREVMKTHDLKFSNRPKTTPTNLLFNGSQDVGFAPYGEYWRRAKKMCVLELFS---VESF 61
           A+EV+KTHD KF+NRP++   + L NG +DV FAPYGEYWR+ K +C+L L +   VESF
Sbjct: 92  AQEVLKTHDHKFANRPRSKAVHGLMNGGRDVVFAPYGEYWRQMKSVCILNLLTNKMVESF 151

Query: 62  QYVRDEEVDVLINMIRKVANCCSVDGVNLSQLFLKISTDIVSRCVMGKKFEDENEKSKYG 121
           + VR++EV+ +I  + K ++  S +  NLS+LF+ + +D+ SR  +G+K  ++       
Sbjct: 152 EKVREDEVNAMIEKLEKASSSSSSE--NLSELFITLPSDVTSRVALGRKHSEDETARDLK 211

Query: 122 DVSRRIMELLAVFFVGNFFSVFGWVDLIRGLVRKIKIVSKKMDEFFDLVIEDHITKLKSS 181
              R+IMELL  F +G +  +  W+D IRG   KIK VS+   +  D V+++H+      
Sbjct: 212 KRVRQIMELLGEFPIGEYVPILAWIDGIRGFNNKIKEVSRGFSDLMDKVVQEHL------ 271

Query: 182 GKSDEHKKDFADIML 194
            ++   K DF DI+L
Sbjct: 272 -EASNDKADFVDILL 277

[Close]

BLAST of Lsi05G007430 vs. TAIR10
Match: AT1G11610.2 (AT1G11610.2 cytochrome P450, family 71, subfamily A, polypeptide 18)

HSP 1 Score: 153.3 bits (386), Expect = 1.5e-37
Identity = 77/195 (39.49%), Postives = 120/195 (61.54%), Query Frame = 1


Query: 2   AREVMKTHDLKFSNRPKTTPTNLLFNGSQDVGFAPYGEYWRRAKKMCVLELFS---VESF 61
           A E++KTHDLKF+NRPK+   + L NG +DV F PYGEYWR+ K +C+L L +   V SF
Sbjct: 86  AHEILKTHDLKFANRPKSKAVHGLMNGGRDVVFGPYGEYWRQMKSVCILNLLTNKMVASF 145

Query: 62  QYVRDEEVDVLINMIRKVANCCSVDGVNLSQLFLKISTDIVSRCVMGKKFEDENEKSKYG 121
           + VR+EEV+ ++  + K +  CS    NLS+LF+ +++D+ SR  +GKK+ ++       
Sbjct: 146 EKVREEEVNAMMEKLEKAS--CSSSAENLSELFVTLTSDVTSRVSLGKKYWEDETAGGLK 205

Query: 122 DVSRRIMELLAVFFVGNFFSVFGWVDLIRGLVRKIKIVSKKMDEFFDLVIEDHITKLKSS 181
              R+IMELL  F +G++     W+D I G   KI  VS+   +  + V+++H+      
Sbjct: 206 KRVRQIMELLREFPIGDYVPALAWIDRINGFNSKIVEVSRAYSDLMEKVVQEHL------ 265

Query: 182 GKSDEHKKDFADIML 194
            ++ EHK DF +I+L
Sbjct: 266 -EAGEHKADFVNILL 271

[Close]

BLAST of Lsi05G007430 vs. TAIR10
Match: AT2G30750.1 (AT2G30750.1 cytochrome P450, family 71, subfamily A, polypeptide 12)

HSP 1 Score: 151.8 bits (382), Expect = 4.5e-37
Identity = 75/195 (38.46%), Postives = 121/195 (62.05%), Query Frame = 1


Query: 2   AREVMKTHDLKFSNRPKTTPTNLLFNGSQDVGFAPYGEYWRRAKKMCVLELFS---VESF 61
           A+EV+KTHDLKF+NRP++   + L NG +DV F PYGEYWR+ K +C+L L +   V SF
Sbjct: 92  AQEVLKTHDLKFANRPRSKAVHGLMNGGRDVVFGPYGEYWRQMKSVCILNLLTNKMVASF 151

Query: 62  QYVRDEEVDVLINMIRKVANCCSVDGVNLSQLFLKISTDIVSRCVMGKKFEDENEKSKYG 121
           + +R+EE++ +I  + K ++  S +  NLS+LF+ + +D+ SR  +G+K  ++       
Sbjct: 152 EKIREEELNEMIKKLEKASSSSSSE--NLSELFVTLPSDVTSRIALGRKHSEDETARDLK 211

Query: 122 DVSRRIMELLAVFFVGNFFSVFGWVDLIRGLVRKIKIVSKKMDEFFDLVIEDHITKLKSS 181
              R+IMELL  F +G++     W+D I G   +IK VS+   +  D V+++H+      
Sbjct: 212 KRVRQIMELLGEFPIGDYVPALAWIDRINGFNARIKEVSQGFSDLMDKVVQEHL------ 271

Query: 182 GKSDEHKKDFADIML 194
            ++  HK+DF DI+L
Sbjct: 272 -EAGNHKEDFVDILL 277

[Close]

BLAST of Lsi05G007430 vs. TAIR10
Match: AT4G13310.1 (AT4G13310.1 cytochrome P450, family 71, subfamily A, polypeptide 20)

HSP 1 Score: 143.7 bits (361), Expect = 1.2e-34
Identity = 66/174 (37.93%), Postives = 107/174 (61.49%), Query Frame = 1


Query: 1   MAREVMKTHDLKFSNRPKTTPTNLLFNGSQDVGFAPYGEYWRRAKKMCVLELFS---VES 60
           +A +VMKTHDL  +NRPKT   + + +G +DV FAPYGEYWR+ K +C+  L +   V S
Sbjct: 84  VAHDVMKTHDLVCANRPKTKVVDKILSGGRDVAFAPYGEYWRQMKSICIQNLLNNKMVRS 143

Query: 61  FQYVRDEEVDVLINMIRKVANCCSVDGVNLSQLFLKISTDIVSRCVMGKKFEDENEKSKY 120
           ++ +R+EE+  +I  + K +   S   VNLSQ+ + ++ DI+ R  +G+K+  + +    
Sbjct: 144 YEKIREEEIKRMIEKLEKASCSSSPSPVNLSQILMTLTNDIICRVALGRKYSGKKDGIDV 203

Query: 121 GDVSRRIMELLAVFFVGNFFSVFGWVDLIRGLVRKIKIVSKKMDEFFDLVIEDH 172
            ++ R    LL  F VG +     W+D IRGL  K+++V K+ DEF + V+++H
Sbjct: 204 ENIVRTFAALLGEFPVGEYIPSLSWIDRIRGLDHKMEVVDKRFDEFLERVVKEH 257

[Close]

BLAST of Lsi05G007430 vs. TAIR10
Match: AT3G48290.2 (AT3G48290.2 cytochrome P450, family 71, subfamily A, polypeptide 24)

HSP 1 Score: 141.7 bits (356), Expect = 4.6e-34
Identity = 73/195 (37.44%), Postives = 118/195 (60.51%), Query Frame = 1


Query: 2   AREVMKTHDLKFSNRPKTTPTNLLFNGSQDVGFAPYGEYWRRAKKMCVLELFS---VESF 61
           A++V+KTHD  F++RP++   + +F   +DV  APYGEYWR+ K +CVL LFS   V SF
Sbjct: 84  AKDVLKTHDRVFASRPRSKIFDKIFYNGRDVALAPYGEYWRQMKSVCVLHLFSNKMVRSF 143

Query: 62  QYVRDEEVDVLINMIRKVANCCSVDGVNLSQLFLKISTDIVSRCVMGKKFEDENEKSKYG 121
           + VR EE+ ++I  IR      S   +NLS++ + ++ +++ R  +G+K+     K+ + 
Sbjct: 144 RDVRQEEISLMIEKIR----ISSSLRINLSEILVNLTNNVICRVALGRKY---GGKTDFK 203

Query: 122 DVSRRIMELLAVFFVGNFFSVFGWVDLIRGLVRKIKIVSKKMDEFFDLVIEDHITKLKSS 181
           D+ +R+  LL  F VG++ S   W+D IRGL  ++  +S  +DEF + V++DH+      
Sbjct: 204 DLMKRLTRLLGEFSVGSYVSWLAWIDWIRGLDGQLIKISNDLDEFLERVVQDHV------ 263

Query: 182 GKSDEHKKDFADIML 194
              D HK DF D +L
Sbjct: 264 -DGDGHKNDFVDFLL 264

[Close]

BLAST of Lsi05G007430 vs. NCBI nr
Match: gi|659067363|ref|XP_008439061.1| (PREDICTED: cytochrome P450 71A1-like [Cucumis melo])

HSP 1 Score: 278.1 bits (710), Expect = 1.2e-71
Identity = 144/197 (73.10%), Postives = 166/197 (84.26%), Query Frame = 1


Query: 1   MAREVMKTHDLKFSNRPKTTPTNLLFNGSQDVGFAPYGEYWRRAKKMCVLELFS---VES 60
           +A E+MKTHDLKFSNRPKTT TNL+F G QDVGFAPYG+YWR+AKKMCVLELFS   VES
Sbjct: 18  VAGEIMKTHDLKFSNRPKTTATNLMFYGCQDVGFAPYGDYWRKAKKMCVLELFSHKRVES 77

Query: 61  FQYVRDEEVDVLINMIRKVANCCSVDGVNLSQLFLKISTDIVSRCVMGKKFEDENEKSKY 120
           FQYVRDEEVD+LIN IRK ANC   +G+NL+QL  +IS DIVSRCV+GKKFEDEN KS++
Sbjct: 78  FQYVRDEEVDILINKIRKRANCS--EGLNLTQLLFQISNDIVSRCVLGKKFEDENGKSEF 137

Query: 121 GDV-SRRIMELLAVFFVGNFFSVFGWVDLIRGLVRKIKIVSKKMDEFFDLVIEDHITKLK 180
            DV SRR+MELLA F VG+FF  FGWVD+IRG+V ++K VSK MD FFD+VIEDHI KLK
Sbjct: 138 RDVHSRRVMELLAAFCVGDFFPNFGWVDVIRGVVGEMKKVSKIMDGFFDMVIEDHIIKLK 197

Query: 181 SSGKSDEHKKDFADIML 194
           SS + D+ KKDF DIML
Sbjct: 198 SSDRCDD-KKDFVDIML 211

[Close]

BLAST of Lsi05G007430 vs. NCBI nr
Match: gi|778657943|ref|XP_004152573.2| (PREDICTED: cytochrome P450 71A1-like [Cucumis sativus])

HSP 1 Score: 260.8 bits (665), Expect = 1.9e-66
Identity = 134/197 (68.02%), Postives = 161/197 (81.73%), Query Frame = 1


Query: 1   MAREVMKTHDLKFSNRPKTTPTNLLFNGSQDVGFAPYGEYWRRAKKMCVLELFS---VES 60
           MARE+MKTHDL FSNRPKTT  N++F   QDVGF+PY + W++AKKMCV+ELFS   VES
Sbjct: 102 MAREIMKTHDLNFSNRPKTTAPNVMFYSCQDVGFSPYSDCWKKAKKMCVVELFSHKRVES 161

Query: 61  FQYVRDEEVDVLINMIRKVANCCSVDGVNLSQLFLKISTDIVSRCVMGKKFEDENEKSKY 120
           F+YVRDEEVD+LIN IRK ANC   +G+NL+QL  + S DIVSRCV+GKKFEDEN K+++
Sbjct: 162 FRYVRDEEVDILINKIRKGANCS--EGLNLTQLLFQTSNDIVSRCVLGKKFEDENGKNEF 221

Query: 121 GDV-SRRIMELLAVFFVGNFFSVFGWVDLIRGLVRKIKIVSKKMDEFFDLVIEDHITKLK 180
            DV SRR+MELL+ F VG+FF  FGWVD+IRGLV ++K VSK MD FFD+VIEDHI KLK
Sbjct: 222 RDVHSRRVMELLSAFCVGDFFPNFGWVDVIRGLVGEMKKVSKIMDGFFDMVIEDHIIKLK 281

Query: 181 SSGKSDEHKKDFADIML 194
           SS + D+ KKDF DIML
Sbjct: 282 SSDRCDD-KKDFVDIML 295

[Close]

BLAST of Lsi05G007430 vs. NCBI nr
Match: gi|778664798|ref|XP_011660355.1| (PREDICTED: cytochrome P450 71A1-like [Cucumis sativus])

HSP 1 Score: 211.5 bits (537), Expect = 1.3e-51
Identity = 111/202 (54.95%), Postives = 141/202 (69.80%), Query Frame = 1


Query: 1   MAREVMKTHDLKFSNRPKTTPTNLLFNGSQDVGFAPYGEYWRRAKKMCVLELFS---VES 60
           MA +VMKTHDL FSNRP+TT    L  G QD+GFAPYGEYWR+A+K+C LELFS   VES
Sbjct: 82  MAEQVMKTHDLVFSNRPQTTAAKTLLYGCQDMGFAPYGEYWRQARKICALELFSVKRVES 141

Query: 61  FQYVRDEEVDVLINMIRKVANCCSVDGVNLSQLFLKISTDIVSRCVMGKKFEDENEKSKY 120
           FQYVRDEE+D L+N IRK  +  S+D   L  LF + S +IVSRCVMG+KFEDE+ KS++
Sbjct: 142 FQYVRDEEIDSLVNKIRKSGSDGSLD---LGHLFFRTSNNIVSRCVMGEKFEDEDGKSRF 201

Query: 121 GDVSRRIMELLAVFFVGNFFSVFGW-VDLIRGLVRKIKIVSKKMDEFFDLVIEDHITKLK 180
            ++SR++M ++  F   +FF  FGW +D+IRG    +K   K +DEFF  V+E+H  K+K
Sbjct: 202 EEISRKVMVIITAFCFEDFFPSFGWIIDVIRGFHWDLKNCFKTLDEFFSKVVEEHKEKIK 261

Query: 181 SSG-----KSDEHKKDFADIML 194
            SG        E KKDF DIML
Sbjct: 262 RSGGVINIDDYESKKDFVDIML 280

[Close]

BLAST of Lsi05G007430 vs. NCBI nr
Match: gi|778657940|ref|XP_011651837.1| (PREDICTED: cytochrome P450 71A1-like [Cucumis sativus])

HSP 1 Score: 209.9 bits (533), Expect = 3.9e-51
Identity = 112/202 (55.45%), Postives = 142/202 (70.30%), Query Frame = 1


Query: 1   MAREVMKTHDLKFSNRPKTTPTNLLFNGSQDVGFAPYGEYWRRAKKMCVLELFS---VES 60
           +A +VMKTH L FSNRP+TT    L  G QDVGFAPYGEYWR+A+K+CVLELFS   VES
Sbjct: 82  IAEQVMKTHVLVFSNRPQTTAAKTLLYGCQDVGFAPYGEYWRQARKICVLELFSVKRVES 141

Query: 61  FQYVRDEEVDVLINMIRKVANCCSVDGVNLSQLFLKISTDIVSRCVMGKKFEDENEKSKY 120
           FQYVRDEE+D LIN IRK+ +  S+D   L  LF + S +IVSRCV+G+KFEDE+ KS++
Sbjct: 142 FQYVRDEEIDSLINKIRKIGSDQSLD---LGHLFFQTSNNIVSRCVLGEKFEDEDGKSRF 201

Query: 121 GDVSRRIMELLAVFFVGNFFSVFGW-VDLIRGLVRKIKIVSKKMDEFFDLVIEDHITKLK 180
            ++SR+ M ++  F V +FF  FGW +D+IRG    +K   K +DEFF  V+E+H  K+K
Sbjct: 202 EEISRKAMVIVTAFCVEDFFPSFGWIIDVIRGFHWDLKNCFKTLDEFFSKVVEEHKEKIK 261

Query: 181 SSG-----KSDEHKKDFADIML 194
            SG        E KKDF DIML
Sbjct: 262 RSGGVINIDDYESKKDFVDIML 280

[Close]

BLAST of Lsi05G007430 vs. NCBI nr
Match: gi|778657937|ref|XP_011651833.1| (PREDICTED: cytochrome P450 71A1-like [Cucumis sativus])

HSP 1 Score: 201.8 bits (512), Expect = 1.1e-48
Identity = 99/196 (50.51%), Postives = 136/196 (69.39%), Query Frame = 1


Query: 1   MAREVMKTHDLKFSNRPKTTPTNLLFNGSQDVGFAPYGEYWRRAKKMCVLELFS---VES 60
           MARE+ K HD+ FSNRPK+T  NL F G +DVGF+PYGEYWR  KK+C LEL S   V+ 
Sbjct: 83  MAREIFKNHDITFSNRPKSTAANLFFYGYKDVGFSPYGEYWRGLKKICTLELLSQRRVQG 142

Query: 61  FQYVRDEEVDVLINMIRKVANCCSVDGVNLSQLFLKISTDIVSRCVMGKKFEDENEKSKY 120
           FQYVR+EEV++L++ + K  +      VNLS L    S +I+SRC+ G+KFEDEN KS++
Sbjct: 143 FQYVREEEVEILVSKVHKATS--EGVSVNLSDLITSTSNNIISRCIFGEKFEDENGKSRF 202

Query: 121 GDVSRRIMELLAVFFVGNFFSVFGWVDLIRGLVRKIKIVSKKMDEFFDLVIEDHITKLKS 180
           G+++R++ +L+  F VG+FF  FGWVD I GL+ K+K  S  +D F +  I +H TK K 
Sbjct: 203 GELTRKMAKLVVGFSVGDFFPAFGWVDNITGLIGKLKETSGALDAFLEQFIAEHKTKKKD 262

Query: 181 SGKSDEHKKDFADIML 194
             +SD  ++DF DI+L
Sbjct: 263 DFRSD--REDFVDILL 274

The following BLAST results are available for this feature:

BLAST of Lsi05G007430 vs. Swiss-Prot


Match Name	E-value	Identity	Description
C71A1_PERAE	1.1e-40	40.61	Cytochrome P450 71A1 OS=Persea americana GN=CYP71A1 PE=1 SV=2	[more]
C71AS_ARATH	5.5e-37	41.33	Putative cytochrome P450 71A28 OS=Arabidopsis thaliana GN=CYP71A28 PE=3 SV=2	[more]
C71AD_ARATH	1.2e-36	40.00	Indoleacetaldoxime dehydratase OS=Arabidopsis thaliana GN=CYP71A13 PE=1 SV=1	[more]
C71AI_ARATH	2.7e-36	39.49	Cytochrome P450 71A18 OS=Arabidopsis thaliana GN=CYP71A18 PE=2 SV=2	[more]
C71AC_ARATH	7.9e-36	38.46	Cytochrome P450 71A12 OS=Arabidopsis thaliana GN=CYP71A12 PE=2 SV=1	[more]

BLAST of Lsi05G007430 vs. TrEMBL


Match Name	E-value	Identity	Description
W9RJY0_9ROSA	6.1e-43	43.65	Cytochrome P450 71A1 OS=Morus notabilis GN=L484_018966 PE=3 SV=1	[more]
A0A061FYF5_THECC	3.3e-41	45.23	Cytochrome P450 71A1 OS=Theobroma cacao GN=TCM_014496 PE=3 SV=1	[more]
V4TQZ9_9ROSI	4.4e-41	43.24	Uncharacterized protein OS=Citrus clementina GN=CICLE_v10024390mg PE=3 SV=1	[more]
A0A0D2VQR5_GOSRA	5.7e-41	43.88	Uncharacterized protein (Fragment) OS=Gossypium raimondii GN=B456_011G232800 PE=...	[more]
V4T6H8_9ROSI	5.7e-41	45.10	Uncharacterized protein OS=Citrus clementina GN=CICLE_v10019648mg PE=3 SV=1	[more]

BLAST of Lsi05G007430 vs. TAIR10


Match Name	E-value	Identity	Description
AT2G30770.1	6.9e-38	40.00	cytochrome P450, family 71, subfamily A, polypeptide 13	[more]
AT1G11610.2	1.5e-37	39.49	cytochrome P450, family 71, subfamily A, polypeptide 18	[more]
AT2G30750.1	4.5e-37	38.46	cytochrome P450, family 71, subfamily A, polypeptide 12	[more]
AT4G13310.1	1.2e-34	37.93	cytochrome P450, family 71, subfamily A, polypeptide 20	[more]
AT3G48290.2	4.6e-34	37.44	cytochrome P450, family 71, subfamily A, polypeptide 24	[more]

BLAST of Lsi05G007430 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659067363\|ref\|XP_008439061.1\|	1.2e-71	73.10	PREDICTED: cytochrome P450 71A1-like [Cucumis melo]	[more]
gi\|778657943\|ref\|XP_004152573.2\|	1.9e-66	68.02	PREDICTED: cytochrome P450 71A1-like [Cucumis sativus]	[more]
gi\|778664798\|ref\|XP_011660355.1\|	1.3e-51	54.95	PREDICTED: cytochrome P450 71A1-like [Cucumis sativus]	[more]
gi\|778657940\|ref\|XP_011651837.1\|	3.9e-51	55.45	PREDICTED: cytochrome P450 71A1-like [Cucumis sativus]	[more]
gi\|778657937\|ref\|XP_011651833.1\|	1.1e-48	50.51	PREDICTED: cytochrome P450 71A1-like [Cucumis sativus]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Biological Process**
Term	Definition
GO:0055114	oxidation-reduction process

Vocabulary: **Molecular Function**
Term	Definition
GO:0020037	heme binding
GO:0005506	iron ion binding
GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen

Vocabulary: **INTERPRO**
Term	Definition
IPR001128	Cyt_P450

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055114	oxidation-reduction process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi05G007430.1	Lsi05G007430.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	GENE3D	G3DSA:1.10.630.10		coord: 2..193 score: 7.3
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 1..193 score: 6.6
IPR001128	Cytochrome P450	unknown	SSF48264	Cytochrome P450	coord: 1..193 score: 9.3
None	No IPR available	PANTHER	PTHR24298	FAMILY NOT NAMED	coord: 1..193 score: 2.1
None	No IPR available	PANTHER	PTHR24298:SF86	CYTOCHROME P450 71B21-RELATED	coord: 1..193 score: 2.1

Synteny

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi05G007430	Cucumber (Chinese Long) v3	cuclsiB217
Lsi05G007430	Cucumber (Chinese Long) v3	cuclsiB235
Lsi05G007430	Watermelon (97103) v2	lsiwmbB323
Lsi05G007430	Watermelon (97103) v2	lsiwmbB322
Lsi05G007430	Wax gourd	lsiwgoB410
Lsi05G007430	Wax gourd	lsiwgoB414
Lsi05G007430	Cucumber (Gy14) v1	cgylsiB075
Lsi05G007430	Cucumber (Gy14) v1	cgylsiB159
Lsi05G007430	Cucurbita maxima (Rimu)	cmalsiB313
Lsi05G007430	Cucurbita maxima (Rimu)	cmalsiB315
Lsi05G007430	Cucurbita maxima (Rimu)	cmalsiB687
Lsi05G007430	Cucurbita moschata (Rifu)	cmolsiB302
Lsi05G007430	Cucurbita moschata (Rifu)	cmolsiB305
Lsi05G007430	Cucurbita moschata (Rifu)	cmolsiB680
Lsi05G007430	Cucurbita pepo (Zucchini)	cpelsiB173
Lsi05G007430	Cucurbita pepo (Zucchini)	cpelsiB176
Lsi05G007430	Cucurbita pepo (Zucchini)	cpelsiB366
Lsi05G007430	Cucurbita pepo (Zucchini)	cpelsiB370
Lsi05G007430	Wild cucumber (PI 183967)	cpilsiB197
Lsi05G007430	Wild cucumber (PI 183967)	cpilsiB215
Lsi05G007430	Cucumber (Chinese Long) v2	culsiB198
Lsi05G007430	Cucumber (Chinese Long) v2	culsiB217
Lsi05G007430	Melon (DHL92) v3.5.1	lsimeB371
Lsi05G007430	Melon (DHL92) v3.5.1	lsimeB380
Lsi05G007430	Watermelon (Charleston Gray)	lsiwcgB318
Lsi05G007430	Watermelon (Charleston Gray)	lsiwcgB319
Lsi05G007430	Watermelon (97103) v1	lsiwmB355
Lsi05G007430	Cucumber (Gy14) v2	cgyblsiB183
Lsi05G007430	Cucumber (Gy14) v2	cgyblsiB203
Lsi05G007430	Melon (DHL92) v3.6.1	lsimedB387
Lsi05G007430	Melon (DHL92) v3.6.1	lsimedB400
Lsi05G007430	Silver-seed gourd	carlsiB509
Lsi05G007430	Silver-seed gourd	carlsiB614