Lsi05G001180.1 (mRNA) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi05G001180.1
Type	mRNA
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	3'-5' exonuclease domain-containing protein
Location	chr05 : 1880736 .. 1883539 (+)
Sequence length	987

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTATATAAAAACCCATCTTCACGATGAGCTAAGGCTATTGAAGTATGTCTCTACTATTTCCAGCTGTCCATTAAAAAAAACATAAATCCAAGATTTTAGCTACAACTCTTACAATTAGCCTTAGTTACATTAAACCATTAGACTAGATTTACTGATAAAGGAGTTGAAGGGAAGAGCAATCTTGGAAGTTACAGCTTACTATGCACAATTTGAACCAGTTTGATTCCCTTCAACTTCCTTCTTAGGTCAAGGCAGTATATGATAACTGGTTAGAATTTTCTACTATTTATTTCACTGCTTCCTAGATTTTGCAGATTTTTACTCCCCCACCCCCTCCCAAGTATAAATGATGGCAAAGTAAATAACTTGTTACTGTGTGTTGGATCTTCAACTATCCTATTGAGTATGAAATTTGTGATTCTTTATTCTAATTTCCAGGAGATGTTTTAAGGAATGTTTCTGCAGTTCCTTCCACTGAAATGAATATTGGGTTGAAAGTGATTCTGGAAAAGCAAGATGTGCATGAGAAGATATTAAGGACCAAAGTTTCTGAAGCCTCAAACATTATCAGAGCTACTGCATCTGATTTTTCACAGTGTATGGCTAATATAAGGGGAAATTGTTCTGCCTCATCGAGTATAAATTGTATGCATATCGATGAAGATCTTCAGAAAATTGTCAAGAGCTACGGTGAAAAAATTATTTTGAAAGAGTCAGACAAAAAACCCAGGACCTCCAAAAGAAAAGGCAAGAGATCATCGACTATGAAAGTGGTTTGCAGTGAAAAGCAAATCGTGTGTGTTGAAGATTGGCGAGGACCCCCACCATTGGATTTGTCCTTTGGGGGTGATGGATGCCCTAAATTTCTATGTGATGTTATGGTAAATATTTAGGTTTCCTCTTTTCTTCTTGAAGCTTGTAGCCTGTGGGTTTTATTTTCTTAAATGTTACGCAAGGTTGCAAGACAAGAAGAAACTAAAAAAATAGAGCTCTCATATTCTAGTCAAATTCTGAATCATCGAATGAAGCACTTCTAGTAAAAATTGTTAGTGCTCAATATCTTGCTTTAATGAGTGTTTGGTAGGAAAAGGTTTTAAGGGACAAGAACAAGCCTAATGGATGATAAATGTTTACTCCAGGTTGAAGGTCTTGCAAAACATCTACGTTGTGTTGGGATTGATGCTGCAGTTCCATTTTCCAAAAGGCCGGACTCTAGGTTCTAAATTATTAACAGTTTTAATGGCTTACATTTTGCAGATTTTTTCAGATTTAATTTGTTAGTTATCCATGGAGATATTTTCCATGCAGTCAATTGTAATGCTTTATCATATGTAGGGAATTAATTGATCAAGCGTGCAAGGAGAAGAGAGTACTCTTGACCAGGGATGCTAAACTGCTGAGACATGAGTATTTGTTATGTAATCAAATATACAGAGTTAAAAGTCTTCTAAAGAATGAACAGTTACTCGAGGTATTGATGCCAATTGCTTATAATTATGGCCATGGGCAATGGGAGTCAAAAGCATTTGTTCATTAATGGACCAACCTTGGTGGAGATCTTGGAAACAGTTTTGAGGAAGAGCTAAGCCTCTTGAACGTTCCTTAAATTCCTGGATTACCTTCTTTCAACAATAGTTCAAATTACCATATCACCATGCTTTTTTATATCAAGTGCAGGTTCCCTACATTTCAAATTTGGTGGCGATTTATAGGTTGAAATATTTTTGGAGATGAGAATGGAAAAGGAACTTTTAATGAGATCAAGGAAGCAATAAAAATTTCATTAGAACCTAGGGACTTGGGAGTATCTATGTCTTAGCAGTTGAAAGCCTACGACACTTATTGCAGCCTTTTGATTTCTATTCTAACTCTTTTTGGATTGATAATAGATATATTTTTCCCTTCTAGTGAATAGACACCCTTATATAGCTCTGAACTGTTAATTTAGCTTTGCACTGAAAACAAAATCAATTCTCCAGTTAGATTATTTTGCAGATATCGGAGGCCCATTCTAATTCCTCAATCATTTAAATCTGTTGTAGGTTATTGAAACTTTCAATTTGAAAATTAGTGAGGATCAGCTGATGTCTAGATGTACCAAATGCAATGGAAGGTTTATTCAAAAGTCCCTTACAACGGAGGAGGCTGTGCAGGCAGCGAAGGGTTTCCAAAAAATTCCTAACTGTTTGTTTGACAAGAATTTAGAGTTCTGGCAGTGCATGGACTGTCACCAACTATACTGGGAGGTAATCTTCTACAGTGTCGCCGTTCTGCAATGAGAATAACTTTTGAGTGGGGAGGTCTTCATGCACTAAAAAACTATTTGTAACATATTTTTTTTGAGGTTTAACAATTTTAGCTATGCAATTCCAGCGGACAAAATATTGAACTTTCCATAAAGGGACCGGTTGAGGTGAAGAAAAGAATACTAAGGGCAAAGAATGGGACTGCACTAAAAGTACCCAAACAAAACAATAAAGTATAAGACTGACTTGGCTTAGTTTTTTGACTTATTTAATGTCCCCAACTATTAATGTCTCACCGGATAAAGAGCGCACTTCGCATTCTTTAGTTGCACCCTACTCTTCTGTTACATTTAGAAGTGAGAAGGGTAATCGGAATTCACTTCAATATGTACTTGGAAAATTTACATTTTGAAGTTATTAACTTATTAGCATTCCTATCATTTATTGTGGCCTGTGTGTTCTGGTAAATCTAATGACGTCCTCCATATTTTTACAGGGGATACAATATCGCAATGCAATCCAGAAGTTCATTGATGTTTGCAAGTTGAATGACTAG

mRNA sequence

ATGTATATAAAAACCCATCTTCACGATGAGCTAAGGCTATTGAAGTCAAGGCAGTATATGATAACTGGAGATGTTTTAAGGAATGTTTCTGCAGTTCCTTCCACTGAAATGAATATTGGGTTGAAAGTGATTCTGGAAAAGCAAGATGTGCATGAGAAGATATTAAGGACCAAAGTTTCTGAAGCCTCAAACATTATCAGAGCTACTGCATCTGATTTTTCACAGTGTATGGCTAATATAAGGGGAAATTGTTCTGCCTCATCGAGTATAAATTGTATGCATATCGATGAAGATCTTCAGAAAATTGTCAAGAGCTACGGTGAAAAAATTATTTTGAAAGAGTCAGACAAAAAACCCAGGACCTCCAAAAGAAAAGGCAAGAGATCATCGACTATGAAAGTGGTTTGCAGTGAAAAGCAAATCGTGTGTGTTGAAGATTGGCGAGGACCCCCACCATTGGATTTGTCCTTTGGGGGTGATGGATGCCCTAAATTTCTATGTGATGTTATGGTTGAAGGTCTTGCAAAACATCTACGTTGTGTTGGGATTGATGCTGCAGTTCCATTTTCCAAAAGGCCGGACTCTAGGGAATTAATTGATCAAGCGTGCAAGGAGAAGAGAGTACTCTTGACCAGGGATGCTAAACTGCTGAGACATGAGTATTTGTTATGTAATCAAATATACAGAGTTAAAAGTCTTCTAAAGAATGAACAGTTACTCGAGGTTATTGAAACTTTCAATTTGAAAATTAGTGAGGATCAGCTGATGTCTAGATGTACCAAATGCAATGGAAGGTTTATTCAAAAGTCCCTTACAACGGAGGAGGCTGTGCAGGCAGCGAAGGGTTTCCAAAAAATTCCTAACTGTTTGTTTGACAAGAATTTAGAGTTCTGGCAGTGCATGGACTGTCACCAACTATACTGGGAGGGGATACAATATCGCAATGCAATCCAGAAGTTCATTGATGTTTGCAAGTTGAATGACTAG

Coding sequence (CDS)

ATGTATATAAAAACCCATCTTCACGATGAGCTAAGGCTATTGAAGTCAAGGCAGTATATGATAACTGGAGATGTTTTAAGGAATGTTTCTGCAGTTCCTTCCACTGAAATGAATATTGGGTTGAAAGTGATTCTGGAAAAGCAAGATGTGCATGAGAAGATATTAAGGACCAAAGTTTCTGAAGCCTCAAACATTATCAGAGCTACTGCATCTGATTTTTCACAGTGTATGGCTAATATAAGGGGAAATTGTTCTGCCTCATCGAGTATAAATTGTATGCATATCGATGAAGATCTTCAGAAAATTGTCAAGAGCTACGGTGAAAAAATTATTTTGAAAGAGTCAGACAAAAAACCCAGGACCTCCAAAAGAAAAGGCAAGAGATCATCGACTATGAAAGTGGTTTGCAGTGAAAAGCAAATCGTGTGTGTTGAAGATTGGCGAGGACCCCCACCATTGGATTTGTCCTTTGGGGGTGATGGATGCCCTAAATTTCTATGTGATGTTATGGTTGAAGGTCTTGCAAAACATCTACGTTGTGTTGGGATTGATGCTGCAGTTCCATTTTCCAAAAGGCCGGACTCTAGGGAATTAATTGATCAAGCGTGCAAGGAGAAGAGAGTACTCTTGACCAGGGATGCTAAACTGCTGAGACATGAGTATTTGTTATGTAATCAAATATACAGAGTTAAAAGTCTTCTAAAGAATGAACAGTTACTCGAGGTTATTGAAACTTTCAATTTGAAAATTAGTGAGGATCAGCTGATGTCTAGATGTACCAAATGCAATGGAAGGTTTATTCAAAAGTCCCTTACAACGGAGGAGGCTGTGCAGGCAGCGAAGGGTTTCCAAAAAATTCCTAACTGTTTGTTTGACAAGAATTTAGAGTTCTGGCAGTGCATGGACTGTCACCAACTATACTGGGAGGGGATACAATATCGCAATGCAATCCAGAAGTTCATTGATGTTTGCAAGTTGAATGACTAG

Protein sequence

MYIKTHLHDELRLLKSRQYMITGDVLRNVSAVPSTEMNIGLKVILEKQDVHEKILRTKVSEASNIIRATASDFSQCMANIRGNCSASSSINCMHIDEDLQKIVKSYGEKIILKESDKKPRTSKRKGKRSSTMKVVCSEKQIVCVEDWRGPPPLDLSFGGDGCPKFLCDVMVEGLAKHLRCVGIDAAVPFSKRPDSRELIDQACKEKRVLLTRDAKLLRHEYLLCNQIYRVKSLLKNEQLLEVIETFNLKISEDQLMSRCTKCNGRFIQKSLTTEEAVQAAKGFQKIPNCLFDKNLEFWQCMDCHQLYWEGIQYRNAIQKFIDVCKLND

Homology

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BLAST of Lsi05G001180.1 vs. TrEMBL
Match: A0A0A0L6Q0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G238160 PE=4 SV=1)

HSP 1 Score: 552.0 bits (1421), Expect = 5.0e-154
Identity = 276/306 (90.20%), Postives = 287/306 (93.79%), Query Frame = 1


Query: 23  GDVLRNVSAVPSTEMNIGLKVILEKQDVHEKILRTKVSEASNIIRATASDFSQCMANIRG 82
           GDVLRNVSAVPSTEMN GL+VILEKQD+HEKILRTKVSEASNIIRATAS F Q MANIRG
Sbjct: 219 GDVLRNVSAVPSTEMNTGLRVILEKQDMHEKILRTKVSEASNIIRATASGFPQSMANIRG 278

Query: 83  NCSASSSINCMHIDEDLQKIVKSYGEKIILKESDKKPRTSKRKGKRSSTMKVVCSEKQIV 142
            CSASSSINCM +DEDLQK+VKSYGE+IILKESDKKP+TSKRK KRSSTMKV C EKQIV
Sbjct: 279 ICSASSSINCMPMDEDLQKVVKSYGERIILKESDKKPKTSKRKSKRSSTMKVGCIEKQIV 338

Query: 143 CVEDWRGPPPLDLSFGGDGCPKFLCDVMVEGLAKHLRCVGIDAAVPFSKRPDSRELIDQA 202
             EDW GPPP DLS GGDGCPKFLCDVMVEGLAKHLRCVG+DAAVPFSK P SRELIDQA
Sbjct: 339 SDEDWHGPPPWDLSLGGDGCPKFLCDVMVEGLAKHLRCVGVDAAVPFSKNPYSRELIDQA 398

Query: 203 CKEKRVLLTRDAKLLRHEYLLCNQIYRVKSLLKNEQLLEVIETFNLKISEDQLMSRCTKC 262
            KEKRVLLTRDAKLLRH YLL NQIYRVK+LLKNEQLLEVIETF+LKISEDQLMSRCTKC
Sbjct: 399 HKEKRVLLTRDAKLLRHGYLLRNQIYRVKNLLKNEQLLEVIETFDLKISEDQLMSRCTKC 458

Query: 263 NGRFIQKSLTTEEAVQAAKGFQKIPNCLFDKNLEFWQCMDCHQLYWEGIQYRNAIQKFID 322
           NGRFIQKSLTTEEAVQAAKGFQKIPNCLFD+NLEFWQCMDCHQLYWEGIQYRNA+QKFID
Sbjct: 459 NGRFIQKSLTTEEAVQAAKGFQKIPNCLFDQNLEFWQCMDCHQLYWEGIQYRNAVQKFID 518

Query: 323 VCKLND 329
           VCKLND
Sbjct: 519 VCKLND 524

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BLAST of Lsi05G001180.1 vs. TrEMBL
Match: A0A067F0L1_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g009783mg PE=4 SV=1)

HSP 1 Score: 420.2 bits (1079), Expect = 2.3e-114
Identity = 210/306 (68.63%), Postives = 252/306 (82.35%), Query Frame = 1


Query: 23  GDVLRNVSAVPSTEMNIGLKVILEKQDVHEKILRTKVSEASNIIRATASDFSQCMANIRG 82
           G+   ++S + S+ +++GLK ILEK D+  K +R K+ EA +IIRAT S +SQC+    G
Sbjct: 220 GNSCSSISELDSSNLDLGLKGILEKPDIGNKTVRFKLCEALDIIRAT-SYYSQCLP--EG 279

Query: 83  NCSASSSINCMHIDEDLQKIVKSYGEKIILKESDKKPRTSKRKGKRSSTMKVVCSEKQIV 142
             S  S +N M +DE L KIV+ YGEKI+L+E DK P+TSK+KG++ S++ V   EK++ 
Sbjct: 280 VVSRVSYLNTMPMDESLVKIVRKYGEKILLRECDKAPKTSKKKGRKRSSVIVDSREKRLD 339

Query: 143 CVEDWRGPPPLDLSFGGDGCPKFLCDVMVEGLAKHLRCVGIDAAVPFSKRPDSRELIDQA 202
            + DW+GPPP DLS GGDG PKFLCDVMVEGLAKHLRCVGIDAA P SK+P+ RELIDQ 
Sbjct: 340 DIGDWQGPPPWDLSLGGDGYPKFLCDVMVEGLAKHLRCVGIDAATPRSKKPEPRELIDQT 399

Query: 203 CKEKRVLLTRDAKLLRHEYLLCNQIYRVKSLLKNEQLLEVIETFNLKISEDQLMSRCTKC 262
            KEKRVLLTRDAKLLRH+YL+ NQIYRVKSLLKN+QLLEVIE F LKISEDQLMSRCTKC
Sbjct: 400 SKEKRVLLTRDAKLLRHQYLIKNQIYRVKSLLKNQQLLEVIEAFQLKISEDQLMSRCTKC 459

Query: 263 NGRFIQKSLTTEEAVQAAKGFQKIPNCLFDKNLEFWQCMDCHQLYWEGIQYRNAIQKFID 322
           NGRFIQK L+TEEAV+AAKGFQ+IP+CLFDKNL+FWQCMDC+QLYWEG QY NA+QKFID
Sbjct: 460 NGRFIQKPLSTEEAVEAAKGFQRIPDCLFDKNLQFWQCMDCNQLYWEGTQYHNAVQKFID 519

Query: 323 VCKLND 329
           VCKLN+
Sbjct: 520 VCKLNE 522

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BLAST of Lsi05G001180.1 vs. TrEMBL
Match: W9S1T5_9ROSA (Putative exonuclease mut-7-like protein OS=Morus notabilis GN=L484_011852 PE=4 SV=1)

HSP 1 Score: 419.5 bits (1077), Expect = 3.8e-114
Identity = 213/307 (69.38%), Postives = 250/307 (81.43%), Query Frame = 1


Query: 23  GDVLRNVSAVPSTEMNIGLKVILEKQDVHEKILRTKVSEASNIIRATAS-DFSQCMANIR 82
           G++  N++   S   N+GLK ILEK  V EKI+RT   +A +I+RATA  D SQ +A+  
Sbjct: 222 GNLNHNLAEPTSLVSNVGLKEILEKNGVCEKIIRTTFCDALDIVRATACFDISQKIASEE 281

Query: 83  GNCSASSSINCMHIDEDLQKIVKSYGEKIILKESDKKPRTSKRKGKRSSTMKVVCSEKQI 142
           G    S   + + +DE L KIV  YGEK++LKESDKKP+ SKRKGKR S+  ++  EKQ+
Sbjct: 282 GMVLRSQQRSTLPMDESLLKIVNRYGEKLVLKESDKKPKISKRKGKRRSSAGLINKEKQL 341

Query: 143 VCVEDWRGPPPLDLSFGGDGCPKFLCDVMVEGLAKHLRCVGIDAAVPFSKRPDSRELIDQ 202
             +ED +GP P DLS GG+G PKFLCDVMVEGLAKHLRCVGIDAA+P SK+P+ RELI Q
Sbjct: 342 ENLEDGQGPIPWDLSLGGNGSPKFLCDVMVEGLAKHLRCVGIDAAIPHSKKPEPRELIGQ 401

Query: 203 ACKEKRVLLTRDAKLLRHEYLLCNQIYRVKSLLKNEQLLEVIETFNLKISEDQLMSRCTK 262
           A +E RVLLTRDAKLLRH+Y+L NQIYRVKSLLKNEQLLEVIETF LKISEDQLMSRCTK
Sbjct: 402 AHRENRVLLTRDAKLLRHQYILKNQIYRVKSLLKNEQLLEVIETFQLKISEDQLMSRCTK 461

Query: 263 CNGRFIQKSLTTEEAVQAAKGFQKIPNCLFDKNLEFWQCMDCHQLYWEGIQYRNAIQKFI 322
           CNGRFIQK LTTEEAV+AAKGFQ+IPNCLF++NLEFWQCMDC+QLYWEG QY+NA+QKFI
Sbjct: 462 CNGRFIQKPLTTEEAVEAAKGFQRIPNCLFNQNLEFWQCMDCNQLYWEGTQYKNAVQKFI 521

Query: 323 DVCKLND 329
           DVCKLND
Sbjct: 522 DVCKLND 528

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BLAST of Lsi05G001180.1 vs. TrEMBL
Match: A0A067K3P6_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_15506 PE=4 SV=1)

HSP 1 Score: 412.1 bits (1058), Expect = 6.1e-112
Identity = 210/317 (66.25%), Postives = 251/317 (79.18%), Query Frame = 1


Query: 13  LLKSRQYMITGDVLRNVSAVPSTEMNIGLKVILEKQDVHEKILRTKVSEASNIIRATASD 72
           +LK R Y        NV  + S+ +N+GLK I  K D  + + RTK SEA  I++AT S 
Sbjct: 209 VLKERSY-------NNVMELHSSSLNLGLKEIFRKHDNGDILTRTKFSEALTILQATVSS 268

Query: 73  FSQC-MANIRGNCSASSSINCMHIDEDLQKIVKSYGEKIILKESDKKPRTSKRKGKRSST 132
              C +A   G  S +SS + M +DE +  IV+ YGEKI+LKESD+KP++SK+K K+ S+
Sbjct: 269 EDSCRIAKREGIISIASSRSTMPMDELILNIVRKYGEKILLKESDRKPKSSKKKEKKRSS 328

Query: 133 MKVVCSEKQIVCVEDWRGPPPLDLSFGGDGCPKFLCDVMVEGLAKHLRCVGIDAAVPFSK 192
           +  +  EKQ+  V DW+GPPP  LS GGDGCPKFLCDVMVEGLAKHLRCVGIDAA+P+SK
Sbjct: 329 V-AIGREKQLDNVGDWQGPPPWVLSLGGDGCPKFLCDVMVEGLAKHLRCVGIDAAIPYSK 388

Query: 193 RPDSRELIDQACKEKRVLLTRDAKLLRHEYLLCNQIYRVKSLLKNEQLLEVIETFNLKIS 252
           +P SRELIDQA KE+RVLLTRDAKLLR++YL  NQIYRVKSLLKN+QLLE+IE F L IS
Sbjct: 389 KPQSRELIDQAIKEQRVLLTRDAKLLRYQYLTSNQIYRVKSLLKNDQLLEIIEAFQLTIS 448

Query: 253 EDQLMSRCTKCNGRFIQKSLTTEEAVQAAKGFQKIPNCLFDKNLEFWQCMDCHQLYWEGI 312
           EDQLMSRCTKCNG FIQK LTTEEAV+AAKGFQKIP+CLF+KNLEFWQCMDC+QLYWEG 
Sbjct: 449 EDQLMSRCTKCNGIFIQKPLTTEEAVEAAKGFQKIPDCLFNKNLEFWQCMDCNQLYWEGT 508

Query: 313 QYRNAIQKFIDVCKLND 329
           QY+NA+QKFID+CKLND
Sbjct: 509 QYQNAVQKFIDICKLND 517

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BLAST of Lsi05G001180.1 vs. TrEMBL
Match: B9RPI0_RICCO (3-5 exonuclease, putative OS=Ricinus communis GN=RCOM_1377360 PE=4 SV=1)

HSP 1 Score: 411.8 bits (1057), Expect = 8.0e-112
Identity = 209/308 (67.86%), Postives = 254/308 (82.47%), Query Frame = 1


Query: 23  GDVLRNVSAVPSTEM-NIGLKVILEKQDVHEKILRTKVSEASNIIRAT-ASDFSQCMANI 82
           G+   NV  + S+ + + GLK ILE+ D  + ++RT+ SEA NII+AT AS+ S  +A  
Sbjct: 217 GEFYNNVMELQSSNIISHGLKEILERFDAGDALIRTRFSEALNIIQATVASEDSHRIARG 276

Query: 83  RGNCSASSSINCMHIDEDLQKIVKSYGEKIILKESDKKPRTSKRKGKRSSTMKVVCSEKQ 142
               S +SSIN + +DE L  IV+ +GEKI+L ESD+KP+  ++KGK+ S++ V C EK 
Sbjct: 277 ERVVSITSSINTLPMDELLLNIVRRFGEKIVLGESDRKPKAFRKKGKKRSSI-VTCREKL 336

Query: 143 IVCVEDWRGPPPLDLSFGGDGCPKFLCDVMVEGLAKHLRCVGIDAAVPFSKRPDSRELID 202
           +  + DW+GPPP D S GGDGCPKFLCDVMVEGLAKHLRCVGIDAA+P+SK+P+SRELID
Sbjct: 337 LGNICDWQGPPPWDFSLGGDGCPKFLCDVMVEGLAKHLRCVGIDAAIPYSKKPESRELID 396

Query: 203 QACKEKRVLLTRDAKLLRHEYLLCNQIYRVKSLLKNEQLLEVIETFNLKISEDQLMSRCT 262
           QA KE RVLLTRDAKLLR +YL+ NQIYRVKSLLKNEQLLEVIETF L+ISEDQLMSRCT
Sbjct: 397 QARKEHRVLLTRDAKLLRFQYLIKNQIYRVKSLLKNEQLLEVIETFKLQISEDQLMSRCT 456

Query: 263 KCNGRFIQKSLTTEEAVQAAKGFQKIPNCLFDKNLEFWQCMDCHQLYWEGIQYRNAIQKF 322
           KCNGRFIQK L+TEEAV+AAKGFQKIP+CLF+KNLEFWQCMDC+QLYWEG QY+NA+QKF
Sbjct: 457 KCNGRFIQKPLSTEEAVEAAKGFQKIPDCLFNKNLEFWQCMDCNQLYWEGTQYQNAVQKF 516

Query: 323 IDVCKLND 329
           ID+CKLN+
Sbjct: 517 IDICKLNE 523

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BLAST of Lsi05G001180.1 vs. TAIR10
Match: AT5G24340.1 (AT5G24340.1 3'-5' exonuclease domain-containing protein)

HSP 1 Score: 381.3 bits (978), Expect = 5.9e-106
Identity = 193/329 (58.66%), Postives = 251/329 (76.29%), Query Frame = 1


Query: 1   MYIKTHLHDELRLLKSRQ-YMITGDVLRNVSAVPSTEMNIGLKVILEKQDVHEKILRTKV 60
           +Y  T  H  L++    + +++ G  ++++  +     N+GL+ IL + D   KI+  K+
Sbjct: 185 LYAATDAHCLLQIFDVFEAHLVEGITVQDLRVI-----NVGLQEILTESDYSSKIVTVKL 244

Query: 61  SEASNIIRATASDFSQCMANIRGNCSASSSINCMHIDEDLQKIVKSYGEKIILKESDKKP 120
            +A+++IR+  S+  Q +AN  G     +++N M +DE+L KIV+ +GE+I+LKESD  P
Sbjct: 245 CKATDVIRSM-SENGQNIAN--GVVPRKTTLNTMPMDENLLKIVRKFGERILLKESDLLP 304

Query: 121 RTSKRKGKRSSTMKVVCSEKQIVCVEDWRGPPPLDLSFGGDGCPKFLCDVMVEGLAKHLR 180
           +  K+K +R      + + KQ+VC  DW+GPPP D S GGDGCPKFL DVMVEGLAKHLR
Sbjct: 305 KKLKKKTRRRVASSTMNTNKQLVCSADWQGPPPWDSSLGGDGCPKFLLDVMVEGLAKHLR 364

Query: 181 CVGIDAAVPFSKRPDSRELIDQACKEKRVLLTRDAKLLRHEYLLCNQIYRVKSLLKNEQL 240
           CVGIDAA+P SK+PDSREL+DQA KE RVLLTRD KLLRH+ L  +QIYRVKSLLKNEQL
Sbjct: 365 CVGIDAAIPHSKKPDSRELLDQAFKENRVLLTRDTKLLRHQDLAKHQIYRVKSLLKNEQL 424

Query: 241 LEVIETFNLKISEDQLMSRCTKCNGRFIQKSLTTEEAVQAAKGFQKIPNCLFDKNLEFWQ 300
           LEVIETF LKIS +QLMSRCTKCNG+FIQK L+ EEA++AAKGFQ+IPNCLF+KNLEFWQ
Sbjct: 425 LEVIETFQLKISGNQLMSRCTKCNGKFIQKPLSIEEAIEAAKGFQRIPNCLFNKNLEFWQ 484

Query: 301 CMDCHQLYWEGIQYRNAIQKFIDVCKLND 329
           CM+CHQLYWEG QY NA+QKF++VCKL++
Sbjct: 485 CMNCHQLYWEGTQYHNAVQKFMEVCKLSE 505

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BLAST of Lsi05G001180.1 vs. NCBI nr
Match: gi|659111952|ref|XP_008455993.1| (PREDICTED: exonuclease mut-7 homolog [Cucumis melo])

HSP 1 Score: 553.9 bits (1426), Expect = 1.9e-154
Identity = 275/306 (89.87%), Postives = 288/306 (94.12%), Query Frame = 1


Query: 23  GDVLRNVSAVPSTEMNIGLKVILEKQDVHEKILRTKVSEASNIIRATASDFSQCMANIRG 82
           GD+LRNVSAVPSTEMN GLKVILEKQD+H+KILRTKVSEASNIIRATAS F Q MANIRG
Sbjct: 218 GDLLRNVSAVPSTEMNTGLKVILEKQDMHKKILRTKVSEASNIIRATASGFPQSMANIRG 277

Query: 83  NCSASSSINCMHIDEDLQKIVKSYGEKIILKESDKKPRTSKRKGKRSSTMKVVCSEKQIV 142
           NCS SSSINCM +DEDLQK+VKSYGE+I+LKESDKKP+ SKRKGKRSSTMKV CSEKQIV
Sbjct: 278 NCSVSSSINCMPMDEDLQKVVKSYGERIVLKESDKKPKISKRKGKRSSTMKVGCSEKQIV 337

Query: 143 CVEDWRGPPPLDLSFGGDGCPKFLCDVMVEGLAKHLRCVGIDAAVPFSKRPDSRELIDQA 202
             EDW GPPP DLS GGDGCPKFLCDVMVEGLAKHLRCVG+DAAVPFSK P SRELID+A
Sbjct: 338 SDEDWLGPPPWDLSLGGDGCPKFLCDVMVEGLAKHLRCVGVDAAVPFSKNPYSRELIDKA 397

Query: 203 CKEKRVLLTRDAKLLRHEYLLCNQIYRVKSLLKNEQLLEVIETFNLKISEDQLMSRCTKC 262
            KEKRVLLTRDAKLLRH YLL NQIYRVK+LLKNEQLLEVIETFNLKISEDQLMSRCTKC
Sbjct: 398 HKEKRVLLTRDAKLLRHGYLLRNQIYRVKNLLKNEQLLEVIETFNLKISEDQLMSRCTKC 457

Query: 263 NGRFIQKSLTTEEAVQAAKGFQKIPNCLFDKNLEFWQCMDCHQLYWEGIQYRNAIQKFID 322
           NGRFIQKSLTTEEAVQAAKGFQKIPNCLFD+NLEFWQCMDCHQLYWEGIQYRNA+QKFID
Sbjct: 458 NGRFIQKSLTTEEAVQAAKGFQKIPNCLFDQNLEFWQCMDCHQLYWEGIQYRNAVQKFID 517

Query: 323 VCKLND 329
           VCKLND
Sbjct: 518 VCKLND 523

[Close]

BLAST of Lsi05G001180.1 vs. NCBI nr
Match: gi|449456993|ref|XP_004146233.1| (PREDICTED: exonuclease mut-7 homolog [Cucumis sativus])

HSP 1 Score: 552.0 bits (1421), Expect = 7.1e-154
Identity = 276/306 (90.20%), Postives = 287/306 (93.79%), Query Frame = 1


Query: 23  GDVLRNVSAVPSTEMNIGLKVILEKQDVHEKILRTKVSEASNIIRATASDFSQCMANIRG 82
           GDVLRNVSAVPSTEMN GL+VILEKQD+HEKILRTKVSEASNIIRATAS F Q MANIRG
Sbjct: 219 GDVLRNVSAVPSTEMNTGLRVILEKQDMHEKILRTKVSEASNIIRATASGFPQSMANIRG 278

Query: 83  NCSASSSINCMHIDEDLQKIVKSYGEKIILKESDKKPRTSKRKGKRSSTMKVVCSEKQIV 142
            CSASSSINCM +DEDLQK+VKSYGE+IILKESDKKP+TSKRK KRSSTMKV C EKQIV
Sbjct: 279 ICSASSSINCMPMDEDLQKVVKSYGERIILKESDKKPKTSKRKSKRSSTMKVGCIEKQIV 338

Query: 143 CVEDWRGPPPLDLSFGGDGCPKFLCDVMVEGLAKHLRCVGIDAAVPFSKRPDSRELIDQA 202
             EDW GPPP DLS GGDGCPKFLCDVMVEGLAKHLRCVG+DAAVPFSK P SRELIDQA
Sbjct: 339 SDEDWHGPPPWDLSLGGDGCPKFLCDVMVEGLAKHLRCVGVDAAVPFSKNPYSRELIDQA 398

Query: 203 CKEKRVLLTRDAKLLRHEYLLCNQIYRVKSLLKNEQLLEVIETFNLKISEDQLMSRCTKC 262
            KEKRVLLTRDAKLLRH YLL NQIYRVK+LLKNEQLLEVIETF+LKISEDQLMSRCTKC
Sbjct: 399 HKEKRVLLTRDAKLLRHGYLLRNQIYRVKNLLKNEQLLEVIETFDLKISEDQLMSRCTKC 458

Query: 263 NGRFIQKSLTTEEAVQAAKGFQKIPNCLFDKNLEFWQCMDCHQLYWEGIQYRNAIQKFID 322
           NGRFIQKSLTTEEAVQAAKGFQKIPNCLFD+NLEFWQCMDCHQLYWEGIQYRNA+QKFID
Sbjct: 459 NGRFIQKSLTTEEAVQAAKGFQKIPNCLFDQNLEFWQCMDCHQLYWEGIQYRNAVQKFID 518

Query: 323 VCKLND 329
           VCKLND
Sbjct: 519 VCKLND 524

[Close]

BLAST of Lsi05G001180.1 vs. NCBI nr
Match: gi|1009138500|ref|XP_015886621.1| (PREDICTED: uncharacterized protein LOC107421806 [Ziziphus jujuba])

HSP 1 Score: 423.3 bits (1087), Expect = 3.8e-115
Identity = 211/307 (68.73%), Postives = 252/307 (82.08%), Query Frame = 1


Query: 23  GDVLRNVSAVPSTEMNIGLKVILEKQDVHEKILRTKVSEASNIIRATAS-DFSQCMANIR 82
           G++   +S    + +NIGLK ILEK D  +KI R K SEA +I+RATAS D  Q +A + 
Sbjct: 226 GNLNHELSETHPSNVNIGLKEILEKDDGCDKISRLKFSEALHIVRATASSDVFQKIATVD 285

Query: 83  GNCSASSSINCMHIDEDLQKIVKSYGEKIILKESDKKPRTSKRKGKRSSTMKVVCSEKQI 142
           G    S   + + +DE L KIVK YGEKI+LKESDKKP+ +KRKGK+ S++ ++C EK+ 
Sbjct: 286 GLVLKSRQRSTLPMDESLLKIVKKYGEKILLKESDKKPKITKRKGKKRSSVGLICKEKRP 345

Query: 143 VCVEDWRGPPPLDLSFGGDGCPKFLCDVMVEGLAKHLRCVGIDAAVPFSKRPDSRELIDQ 202
              ++ +GPPP DL+ GG+GCPKFLCDVMVEGLAKHLRCVGIDAA+P SK+PDSRELID 
Sbjct: 346 EACDERQGPPPWDLTLGGNGCPKFLCDVMVEGLAKHLRCVGIDAAIPHSKKPDSRELIDH 405

Query: 203 ACKEKRVLLTRDAKLLRHEYLLCNQIYRVKSLLKNEQLLEVIETFNLKISEDQLMSRCTK 262
           A +E RVLLTRDAKLLRHEY++ NQIYRVKSLLKNEQLLEVIETF L ISEDQLMSRCTK
Sbjct: 406 AKRENRVLLTRDAKLLRHEYIIKNQIYRVKSLLKNEQLLEVIETFQLTISEDQLMSRCTK 465

Query: 263 CNGRFIQKSLTTEEAVQAAKGFQKIPNCLFDKNLEFWQCMDCHQLYWEGIQYRNAIQKFI 322
           CNGRFIQK L+TEEA++AAKGFQ+IP+CLFDKNLEFWQCMDC+QLYWEG QY NA+QKFI
Sbjct: 466 CNGRFIQKPLSTEEAIEAAKGFQRIPSCLFDKNLEFWQCMDCNQLYWEGTQYHNAVQKFI 525

Query: 323 DVCKLND 329
           +VCKLN+
Sbjct: 526 NVCKLNE 532

[Close]

BLAST of Lsi05G001180.1 vs. NCBI nr
Match: gi|641840722|gb|KDO59640.1| (hypothetical protein CISIN_1g009783mg [Citrus sinensis])

HSP 1 Score: 420.2 bits (1079), Expect = 3.2e-114
Identity = 210/306 (68.63%), Postives = 252/306 (82.35%), Query Frame = 1


Query: 23  GDVLRNVSAVPSTEMNIGLKVILEKQDVHEKILRTKVSEASNIIRATASDFSQCMANIRG 82
           G+   ++S + S+ +++GLK ILEK D+  K +R K+ EA +IIRAT S +SQC+    G
Sbjct: 220 GNSCSSISELDSSNLDLGLKGILEKPDIGNKTVRFKLCEALDIIRAT-SYYSQCLP--EG 279

Query: 83  NCSASSSINCMHIDEDLQKIVKSYGEKIILKESDKKPRTSKRKGKRSSTMKVVCSEKQIV 142
             S  S +N M +DE L KIV+ YGEKI+L+E DK P+TSK+KG++ S++ V   EK++ 
Sbjct: 280 VVSRVSYLNTMPMDESLVKIVRKYGEKILLRECDKAPKTSKKKGRKRSSVIVDSREKRLD 339

Query: 143 CVEDWRGPPPLDLSFGGDGCPKFLCDVMVEGLAKHLRCVGIDAAVPFSKRPDSRELIDQA 202
            + DW+GPPP DLS GGDG PKFLCDVMVEGLAKHLRCVGIDAA P SK+P+ RELIDQ 
Sbjct: 340 DIGDWQGPPPWDLSLGGDGYPKFLCDVMVEGLAKHLRCVGIDAATPRSKKPEPRELIDQT 399

Query: 203 CKEKRVLLTRDAKLLRHEYLLCNQIYRVKSLLKNEQLLEVIETFNLKISEDQLMSRCTKC 262
            KEKRVLLTRDAKLLRH+YL+ NQIYRVKSLLKN+QLLEVIE F LKISEDQLMSRCTKC
Sbjct: 400 SKEKRVLLTRDAKLLRHQYLIKNQIYRVKSLLKNQQLLEVIEAFQLKISEDQLMSRCTKC 459

Query: 263 NGRFIQKSLTTEEAVQAAKGFQKIPNCLFDKNLEFWQCMDCHQLYWEGIQYRNAIQKFID 322
           NGRFIQK L+TEEAV+AAKGFQ+IP+CLFDKNL+FWQCMDC+QLYWEG QY NA+QKFID
Sbjct: 460 NGRFIQKPLSTEEAVEAAKGFQRIPDCLFDKNLQFWQCMDCNQLYWEGTQYHNAVQKFID 519

Query: 323 VCKLND 329
           VCKLN+
Sbjct: 520 VCKLNE 522

[Close]

BLAST of Lsi05G001180.1 vs. NCBI nr
Match: gi|703147990|ref|XP_010109230.1| (putative exonuclease mut-7-like protein [Morus notabilis])

HSP 1 Score: 419.5 bits (1077), Expect = 5.5e-114
Identity = 213/307 (69.38%), Postives = 250/307 (81.43%), Query Frame = 1


Query: 23  GDVLRNVSAVPSTEMNIGLKVILEKQDVHEKILRTKVSEASNIIRATAS-DFSQCMANIR 82
           G++  N++   S   N+GLK ILEK  V EKI+RT   +A +I+RATA  D SQ +A+  
Sbjct: 222 GNLNHNLAEPTSLVSNVGLKEILEKNGVCEKIIRTTFCDALDIVRATACFDISQKIASEE 281

Query: 83  GNCSASSSINCMHIDEDLQKIVKSYGEKIILKESDKKPRTSKRKGKRSSTMKVVCSEKQI 142
           G    S   + + +DE L KIV  YGEK++LKESDKKP+ SKRKGKR S+  ++  EKQ+
Sbjct: 282 GMVLRSQQRSTLPMDESLLKIVNRYGEKLVLKESDKKPKISKRKGKRRSSAGLINKEKQL 341

Query: 143 VCVEDWRGPPPLDLSFGGDGCPKFLCDVMVEGLAKHLRCVGIDAAVPFSKRPDSRELIDQ 202
             +ED +GP P DLS GG+G PKFLCDVMVEGLAKHLRCVGIDAA+P SK+P+ RELI Q
Sbjct: 342 ENLEDGQGPIPWDLSLGGNGSPKFLCDVMVEGLAKHLRCVGIDAAIPHSKKPEPRELIGQ 401

Query: 203 ACKEKRVLLTRDAKLLRHEYLLCNQIYRVKSLLKNEQLLEVIETFNLKISEDQLMSRCTK 262
           A +E RVLLTRDAKLLRH+Y+L NQIYRVKSLLKNEQLLEVIETF LKISEDQLMSRCTK
Sbjct: 402 AHRENRVLLTRDAKLLRHQYILKNQIYRVKSLLKNEQLLEVIETFQLKISEDQLMSRCTK 461

Query: 263 CNGRFIQKSLTTEEAVQAAKGFQKIPNCLFDKNLEFWQCMDCHQLYWEGIQYRNAIQKFI 322
           CNGRFIQK LTTEEAV+AAKGFQ+IPNCLF++NLEFWQCMDC+QLYWEG QY+NA+QKFI
Sbjct: 462 CNGRFIQKPLTTEEAVEAAKGFQRIPNCLFNQNLEFWQCMDCNQLYWEGTQYKNAVQKFI 521

Query: 323 DVCKLND 329
           DVCKLND
Sbjct: 522 DVCKLND 528

The following BLAST results are available for this feature:

BLAST of Lsi05G001180.1 vs. Swiss-Prot


Match Name	E-value	Identity	Description

BLAST of Lsi05G001180.1 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L6Q0_CUCSA	5.0e-154	90.20	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G238160 PE=4 SV=1	[more]
A0A067F0L1_CITSI	2.3e-114	68.63	Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g009783mg PE=4 SV=1	[more]
W9S1T5_9ROSA	3.8e-114	69.38	Putative exonuclease mut-7-like protein OS=Morus notabilis GN=L484_011852 PE=4 S...	[more]
A0A067K3P6_JATCU	6.1e-112	66.25	Uncharacterized protein OS=Jatropha curcas GN=JCGZ_15506 PE=4 SV=1	[more]
B9RPI0_RICCO	8.0e-112	67.86	3-5 exonuclease, putative OS=Ricinus communis GN=RCOM_1377360 PE=4 SV=1	[more]

BLAST of Lsi05G001180.1 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G24340.1	5.9e-106	58.66	3'-5' exonuclease domain-containing protein	[more]

BLAST of Lsi05G001180.1 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659111952\|ref\|XP_008455993.1\|	1.9e-154	89.87	PREDICTED: exonuclease mut-7 homolog [Cucumis melo]	[more]
gi\|449456993\|ref\|XP_004146233.1\|	7.1e-154	90.20	PREDICTED: exonuclease mut-7 homolog [Cucumis sativus]	[more]
gi\|1009138500\|ref\|XP_015886621.1\|	3.8e-115	68.73	PREDICTED: uncharacterized protein LOC107421806 [Ziziphus jujuba]	[more]
gi\|641840722\|gb\|KDO59640.1\|	3.2e-114	68.63	hypothetical protein CISIN_1g009783mg [Citrus sinensis]	[more]
gi\|703147990\|ref\|XP_010109230.1\|	5.5e-114	69.38	putative exonuclease mut-7-like protein [Morus notabilis]	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **INTERPRO**
Term	Definition
IPR002782	Mut7-C_RNAse_dom

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0090305	nucleic acid phosphodiester bond hydrolysis
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0008408	3'-5' exonuclease activity
molecular_function	GO:0003676	nucleic acid binding

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Lsi05G001180	Lsi05G001180	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Lsi05G001180.1	Lsi05G001180.1-protein	polypeptide

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lsi05G001180.1.CDS.1	Lsi05G001180.1.CDS.1	CDS
Lsi05G001180.1.CDS.2	Lsi05G001180.1.CDS.2	CDS
Lsi05G001180.1.CDS.3	Lsi05G001180.1.CDS.3	CDS
Lsi05G001180.1.CDS.4	Lsi05G001180.1.CDS.4	CDS
Lsi05G001180.1.CDS.5	Lsi05G001180.1.CDS.5	CDS
Lsi05G001180.1.CDS.6	Lsi05G001180.1.CDS.6	CDS
Lsi05G001180.1.CDS.7	Lsi05G001180.1.CDS.7	CDS

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002782	Mut7-C RNAse domain	PFAM	PF01927	Mut7-C	coord: 163..319 score: 1.4
None	No IPR available	PANTHER	PTHR12124	POLYMYOSITIS/SCLERODERMA AUTOANTIGEN-RELATED	coord: 44..328 score: 1.2E
None	No IPR available	PANTHER	PTHR12124:SF38	3'-5' EXONUCLEASE DOMAIN-CONTAINING PROTEIN	coord: 44..328 score: 1.2E