Lsi04G014020.1 (mRNA) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi04G014020.1
Type	mRNA
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	17.9 kDa class II heat shock protein
Location	chr04 : 21787742 .. 21788215 (+)
Sequence length	474

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGGTAGCCAAAATGGGACTCGACCCAATATTCCTGAACACCCTCCACGACCTTCTGGACTTCACGGACGACCCAGGACAGAGCAGCCACCACGCACCGTCACGTGCCTACGTGAGGGACGCAAAGGCGATGGCGGCGACTCCGGCGGACGTGGTGGAATATCCAAACTCATACCAATTCACCATCGACATGCCTGGCCTGAAACCCGACCAAATAAAGGTGAAAATAGAGGACAACCAGTTGGTTGTGAGCGGCGAGAGGAAGAGGGAAAGTGAAAAAGTAAAGGAGGGGAAATTTGTGAGAATGGAGAGGAGGCTCGGAAAATACTTGAAGAAATTTGACCTGCCGGAAACTGCCGACGCCGAGAAAGTGTCGGCGGCGTACCAGGACGGAGTGCTGTCGGTCACCGTGGAGAAGAAGCCGCCGCCGGAGCCCAAAAAGGCTAAGAATATTGAGGTTCATGTGGCGTGA

mRNA sequence

ATGGAGGTAGCCAAAATGGGACTCGACCCAATATTCCTGAACACCCTCCACGACCTTCTGGACTTCACGGACGACCCAGGACAGAGCAGCCACCACGCACCGTCACGTGCCTACGTGAGGGACGCAAAGGCGATGGCGGCGACTCCGGCGGACGTGGTGGAATATCCAAACTCATACCAATTCACCATCGACATGCCTGGCCTGAAACCCGACCAAATAAAGGTGAAAATAGAGGACAACCAGTTGGTTGTGAGCGGCGAGAGGAAGAGGGAAAGTGAAAAAGTAAAGGAGGGGAAATTTGTGAGAATGGAGAGGAGGCTCGGAAAATACTTGAAGAAATTTGACCTGCCGGAAACTGCCGACGCCGAGAAAGTGTCGGCGGCGTACCAGGACGGAGTGCTGTCGGTCACCGTGGAGAAGAAGCCGCCGCCGGAGCCCAAAAAGGCTAAGAATATTGAGGTTCATGTGGCGTGA

Coding sequence (CDS)

ATGGAGGTAGCCAAAATGGGACTCGACCCAATATTCCTGAACACCCTCCACGACCTTCTGGACTTCACGGACGACCCAGGACAGAGCAGCCACCACGCACCGTCACGTGCCTACGTGAGGGACGCAAAGGCGATGGCGGCGACTCCGGCGGACGTGGTGGAATATCCAAACTCATACCAATTCACCATCGACATGCCTGGCCTGAAACCCGACCAAATAAAGGTGAAAATAGAGGACAACCAGTTGGTTGTGAGCGGCGAGAGGAAGAGGGAAAGTGAAAAAGTAAAGGAGGGGAAATTTGTGAGAATGGAGAGGAGGCTCGGAAAATACTTGAAGAAATTTGACCTGCCGGAAACTGCCGACGCCGAGAAAGTGTCGGCGGCGTACCAGGACGGAGTGCTGTCGGTCACCGTGGAGAAGAAGCCGCCGCCGGAGCCCAAAAAGGCTAAGAATATTGAGGTTCATGTGGCGTGA

Protein sequence

MEVAKMGLDPIFLNTLHDLLDFTDDPGQSSHHAPSRAYVRDAKAMAATPADVVEYPNSYQFTIDMPGLKPDQIKVKIEDNQLVVSGERKRESEKVKEGKFVRMERRLGKYLKKFDLPETADAEKVSAAYQDGVLSVTVEKKPPPEPKKAKNIEVHVA

Homology

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BLAST of Lsi04G014020.1 vs. Swiss-Prot
Match: HSP22_IPONI (18.8 kDa class II heat shock protein OS=Ipomoea nil GN=SHSP-2 PE=2 SV=1)

HSP 1 Score: 180.6 bits (457), Expect = 1.3e-44
Identity = 96/160 (60.00%), Postives = 120/160 (75.00%), Query Frame = 1


Query: 3   VAKMGLDPIFLNTLHDLLDFTDD---PGQSSHHAPSRAYVRDAKAMAATPADVVEYPNSY 62
           ++  GL+P  L+T+ D+LDF DD    G++    P RAYVRDAKAMAATPADV EYPNSY
Sbjct: 8   LSNFGLEPQLLSTIQDMLDFADDHDRAGRAPPEQPIRAYVRDAKAMAATPADVKEYPNSY 67

Query: 63  QFTIDMPGLKPDQIKVKIED-NQLVVSGERKRESEKVKEG-KFVRMERRLGKYLKKFDLP 122
            F  DMPG+K  +IKV++ED N LVVSGER    +  K+G K++RMERR+GK+++KF LP
Sbjct: 68  VFIADMPGVKAAEIKVQVEDDNVLVVSGERTEREKDEKDGVKYLRMERRVGKFMRKFVLP 127

Query: 123 ETADAEKVSAAYQDGVLSVTVEKKPPPEPKKAKNIEVHVA 158
           E A+ E ++A YQDGVL VTVEK PPPEPKK K +EV VA
Sbjct: 128 ENANVEAINAVYQDGVLQVTVEKLPPPEPKKPKTVEVKVA 167

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BLAST of Lsi04G014020.1 vs. Swiss-Prot
Match: HSP21_PEA (17.1 kDa class II heat shock protein OS=Pisum sativum GN=HSP17.7 PE=2 SV=1)

HSP 1 Score: 177.2 bits (448), Expect = 1.4e-43
Identity = 95/154 (61.69%), Postives = 118/154 (76.62%), Query Frame = 1


Query: 6   MGLDPIFLNTLHDLLDFTDDPGQSSHHAPSRAYVRDAKAMAATPADVVEYPNSYQFTIDM 65
           M LD    NTLH ++D TDD  + + +AP+R YVRDAKAMAATPADV E+PNSY F +DM
Sbjct: 1   MDLDSPLFNTLHHIMDLTDDTTEKNLNAPTRTYVRDAKAMAATPADVKEHPNSYVFMVDM 60

Query: 66  PGLKPDQIKVKIED-NQLVVSGERKRESEKVKEG-KFVRMERRLGKYLKKFDLPETADAE 125
           PG+K   IKV++ED N L++SGERKRE E  KEG K+++MERR+GK ++KF LPE A+ E
Sbjct: 61  PGVKSGDIKVQVEDENVLLISGERKREEE--KEGVKYLKMERRIGKLMRKFVLPENANIE 120

Query: 126 KVSAAYQDGVLSVTVEKKPPPEPKKAKNIEVHVA 158
            +SA  QDGVL+VTV K PPPEPKK K I+V VA
Sbjct: 121 AISAISQDGVLTVTVNKLPPPEPKKPKTIQVKVA 152

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BLAST of Lsi04G014020.1 vs. Swiss-Prot
Match: HSP21_SOLPE (17.3 kDa class II heat shock protein OS=Solanum peruvianum PE=2 SV=1)

HSP 1 Score: 176.8 bits (447), Expect = 1.9e-43
Identity = 96/160 (60.00%), Postives = 124/160 (77.50%), Query Frame = 1


Query: 1   MEVAKMGLD--PIFLNTLHDLLDFTDDPGQSSHHAPSRAYVRDAKAMAATPADVVEYPNS 60
           M++  +G+D  P+F +TLH +++     G+ S +APS+ YVRDAKAMAATPADV EYPNS
Sbjct: 1   MDLRLLGIDNTPLF-HTLHHMMEAA---GEDSVNAPSKIYVRDAKAMAATPADVKEYPNS 60

Query: 61  YQFTIDMPGLKPDQIKVKI-EDNQLVVSGERKRESEKVKEGKFVRMERRLGKYLKKFDLP 120
           Y F +DMPGLK   IKV++ EDN L++SGERKRE EK +  KF+RMERR+GK+++KF LP
Sbjct: 61  YVFVVDMPGLKSGDIKVQVEEDNVLLISGERKREEEK-EGAKFIRMERRVGKFMRKFSLP 120

Query: 121 ETADAEKVSAAYQDGVLSVTVEKKPPPEPKKAKNIEVHVA 158
           E A+ + +SA  QDGVL+VTV+K PPPEPKK K IEV VA
Sbjct: 121 ENANTDAISAVCQDGVLTVTVQKLPPPEPKKPKTIEVKVA 155

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BLAST of Lsi04G014020.1 vs. Swiss-Prot
Match: HSP21_HELAN (17.9 kDa class II heat shock protein OS=Helianthus annuus GN=HSP17.9 PE=2 SV=1)

HSP 1 Score: 176.8 bits (447), Expect = 1.9e-43
Identity = 100/157 (63.69%), Postives = 121/157 (77.07%), Query Frame = 1


Query: 6   MGLDPIFLNTLHDLLDFTDDP---GQSSHHAPSRAYVRDAKAMAATPADVVEYPNSYQFT 65
           MG DP+  N LH +L+ TDD     +S++  PSRAYVRDA+AMAATPADV E PNSY F 
Sbjct: 7   MGFDPLLRN-LHYILEATDDNTTGNKSNNSGPSRAYVRDARAMAATPADVKECPNSYVFI 66

Query: 66  IDMPGLKPDQIKVKIE-DNQLVVSGERKRESEKVKEG-KFVRMERRLGKYLKKFDLPETA 125
           +DMPGLK   IKV++E DN LV+SG+R RE E  KEG K+VRMERR+GK++KKF LPE A
Sbjct: 67  VDMPGLKSGDIKVQVERDNVLVISGKRNREEE--KEGVKYVRMERRMGKFMKKFALPEDA 126

Query: 126 DAEKVSAAYQDGVLSVTVEKKPPPEPKKAKNIEVHVA 158
           + +K+SA  QDGVL+VTVEK PPPEPKK K I+V VA
Sbjct: 127 NTDKISAICQDGVLTVTVEKLPPPEPKKPKTIQVQVA 160

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BLAST of Lsi04G014020.1 vs. Swiss-Prot
Match: HS176_ARATH (17.6 kDa class II heat shock protein OS=Arabidopsis thaliana GN=HSP17.6 PE=2 SV=1)

HSP 1 Score: 171.8 bits (434), Expect = 6.0e-42
Identity = 89/154 (57.79%), Postives = 121/154 (78.57%), Query Frame = 1


Query: 6   MGLDPIFLNTLHDLLDFTDDPG-QSSHHAPSRAYVRDAKAMAATPADVVEYPNSYQFTID 65
           +G  PI ++ L D+L+  +D   + + + PSR Y+RDAKAMAATPADV+E+PN+Y F +D
Sbjct: 3   LGRFPI-ISILEDMLEVPEDHNNEKTRNNPSRVYMRDAKAMAATPADVIEHPNAYAFVVD 62

Query: 66  MPGLKPDQIKVKIE-DNQLVVSGERKRESEKVKEGKFVRMERRLGKYLKKFDLPETADAE 125
           MPG+K D+IKV++E DN LVVSGER+RE+++ +  K+VRMERR+GK+++KF LPE AD +
Sbjct: 63  MPGIKGDEIKVQVENDNVLVVSGERQRENKENEGVKYVRMERRMGKFMRKFQLPENADLD 122

Query: 126 KVSAAYQDGVLSVTVEKKPPPEPKKAKNIEVHVA 158
           K+SA   DGVL VTV+K PPPEPKK K I+V VA
Sbjct: 123 KISAVCHDGVLKVTVQKLPPPEPKKPKTIQVQVA 155

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BLAST of Lsi04G014020.1 vs. TrEMBL
Match: A0A0A0L5D4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G646350 PE=3 SV=1)

HSP 1 Score: 279.6 bits (714), Expect = 2.3e-72
Identity = 142/158 (89.87%), Postives = 150/158 (94.94%), Query Frame = 1


Query: 1   MEVAKM-GLDPIFLNTLHDLLDFTDDPGQSSHHAPSRAYVRDAKAMAATPADVVEYPNSY 60
           MEVAKM G DP+FLN LHDLLDF+D+PGQ SHHAPSRAY+RDAKAMAATPADVVEYPNSY
Sbjct: 1   MEVAKMPGFDPLFLNALHDLLDFSDEPGQGSHHAPSRAYLRDAKAMAATPADVVEYPNSY 60

Query: 61  QFTIDMPGLKPDQIKVKIEDNQLVVSGERKRESEKVKEGKFVRMERRLGKYLKKFDLPET 120
           QFTIDMPGL  DQIKVK+ED QLVVSGERKRESEKVKEGKFVRMERRLGKYLKKFDLPET
Sbjct: 61  QFTIDMPGLTSDQIKVKVEDGQLVVSGERKRESEKVKEGKFVRMERRLGKYLKKFDLPET 120

Query: 121 ADAEKVSAAYQDGVLSVTVEKKPPPEPKKAKNIEVHVA 158
           ADA+KVSAAY+DGVLSVTVEKKPPPEPKKAK+IEV VA
Sbjct: 121 ADADKVSAAYRDGVLSVTVEKKPPPEPKKAKSIEVRVA 158

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BLAST of Lsi04G014020.1 vs. TrEMBL
Match: A0A059CLA5_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_C00678 PE=3 SV=1)

HSP 1 Score: 219.9 bits (559), Expect = 2.1e-54
Identity = 114/168 (67.86%), Postives = 135/168 (80.36%), Query Frame = 1


Query: 1   MEVAKMGLDPIFLNTLHDLLDFTDDPGQSSHHAPSRAYVRDAKAMAATPADVVEYPNSYQ 60
           M++  MG DP  L+TLH+LLD +D+P   SHHAPSRA++RD KAMAATPADV EYPNSY 
Sbjct: 1   MDLRDMGFDPTVLDTLHELLDLSDEPDAKSHHAPSRAFLRDKKAMAATPADVKEYPNSYV 60

Query: 61  FTIDMPGLKPDQIKVKIED-NQLVVSGERKRESEK----VKEG------KFVRMERRLGK 120
           F +DMPGLKPDQIKV++ED N LVVSGERKRE EK     K+G      ++V+MERRLGK
Sbjct: 61  FVLDMPGLKPDQIKVQVEDGNMLVVSGERKREKEKDLKDQKDGAGGGLLRYVKMERRLGK 120

Query: 121 YLKKFDLPETADAEKVSAAYQDGVLSVTVEKKPPPEPKKAKNIEVHVA 158
           +LKKF LPE AD EK+SA+YQDGVL+VTVEK+PPPEPKK K IEV +A
Sbjct: 121 FLKKFVLPENADPEKISASYQDGVLTVTVEKRPPPEPKKPKTIEVQIA 168

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BLAST of Lsi04G014020.1 vs. TrEMBL
Match: A0A061GKC6_THECC (Heat-shock protein, putative OS=Theobroma cacao GN=TCM_037346 PE=3 SV=1)

HSP 1 Score: 206.5 bits (524), Expect = 2.4e-50
Identity = 107/158 (67.72%), Postives = 128/158 (81.01%), Query Frame = 1


Query: 1   MEVAKMGLDPIFLNTLHDLLDFTDDPGQSSHHAPSRAYVRDAKAMAATPADVVEYPNSYQ 60
           M+   MG DP  ++TLH+LLDF DD  +S  H PSRAY+RDAKAMAATPADV + PN+Y 
Sbjct: 1   MDWRVMGFDPSVIDTLHELLDFPDDADKSQPH-PSRAYIRDAKAMAATPADVKDCPNAYV 60

Query: 61  FTIDMPGLKPDQIKVKIED-NQLVVSGERKRESEKVKEGKFVRMERRLGKYLKKFDLPET 120
             IDMPGLK DQ+KV++E+ N LVVSGERKRE EK +  KF++MERRLGKYLKKF LPE 
Sbjct: 61  VVIDMPGLKQDQLKVQVEEGNFLVVSGERKREKEKDQGVKFIKMERRLGKYLKKFQLPEM 120

Query: 121 ADAEKVSAAYQDGVLSVTVEKKPPPEPKKAKNIEVHVA 158
           AD EK+SA+YQDGVL+VTVEKKPPPEPKK + +EV V+
Sbjct: 121 ADTEKISASYQDGVLTVTVEKKPPPEPKKPRTVEVQVS 157

[Close]

BLAST of Lsi04G014020.1 vs. TrEMBL
Match: W9QXD5_9ROSA (Uncharacterized protein OS=Morus notabilis GN=L484_003776 PE=3 SV=1)

HSP 1 Score: 204.9 bits (520), Expect = 7.1e-50
Identity = 110/159 (69.18%), Postives = 129/159 (81.13%), Query Frame = 1


Query: 6   MGLDPIFLN--TLHDLLDFTDD----PGQSSHHAPSRAYVRDAKAMAATPADVVEYPNSY 65
           MGLDP  L   TLHD+LD++++    PG  +HHAPSRAYVRDAKAMAATPADV + PN+Y
Sbjct: 1   MGLDPAQLIEFTLHDILDYSEETSGGPGGEAHHAPSRAYVRDAKAMAATPADVKDTPNAY 60

Query: 66  QFTIDMPGLKPDQIKVKIED-NQLVVSGERKRESEKVKEGKFVRMERRLGKYLKKFDLPE 125
            F IDMPGLK DQIKV +E+ N LVVSGERKRE EK +  K++RMERRLGKYLKKF LPE
Sbjct: 61  VFVIDMPGLKQDQIKVHVEEENVLVVSGERKREREKDQGVKYLRMERRLGKYLKKFVLPE 120

Query: 126 TADAEKVSAAYQDGVLSVTVEKKPPPEPKKAKNIEVHVA 158
            AD +K+SA+YQDGVL+VT++KKPPPEPKK K  EV VA
Sbjct: 121 NADTDKISASYQDGVLTVTIQKKPPPEPKKPKTSEVQVA 159

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BLAST of Lsi04G014020.1 vs. TrEMBL
Match: B9RQT8_RICCO (Heat-shock protein, putative OS=Ricinus communis GN=RCOM_0706500 PE=3 SV=1)

HSP 1 Score: 203.4 bits (516), Expect = 2.1e-49
Identity = 105/150 (70.00%), Postives = 122/150 (81.33%), Query Frame = 1


Query: 8   LDPIFLNTLHDLLDFTDDPGQSSHHAPSRAYVRDAKAMAATPADVVEYPNSYQFTIDMPG 67
           LDP  L+ LH++L+ +D+  + SHHAPSR YVRD KAMAATPAD  E PN+Y F IDMPG
Sbjct: 10  LDPGILDVLHEILEVSDET-EKSHHAPSRTYVRDTKAMAATPADAKELPNAYMFVIDMPG 69

Query: 68  LKPDQIKVKIED-NQLVVSGERKRESEKVKEGKFVRMERRLGKYLKKFDLPETADAEKVS 127
           LK DQIKV +ED N LVVSGERKRE EK +  +++RMERRLGKYLKKF LPE AD+EK+S
Sbjct: 70  LKADQIKVHVEDGNMLVVSGERKREKEKDQGVRYIRMERRLGKYLKKFVLPENADSEKIS 129

Query: 128 AAYQDGVLSVTVEKKPPPEPKKAKNIEVHV 157
           A YQDGVL+VTVEKKPPPEPKK K +EV V
Sbjct: 130 ATYQDGVLTVTVEKKPPPEPKKPKTVEVQV 158

[Close]

BLAST of Lsi04G014020.1 vs. TAIR10
Match: AT5G12020.1 (AT5G12020.1 17.6 kDa class II heat shock protein)

HSP 1 Score: 171.8 bits (434), Expect = 3.4e-43
Identity = 89/154 (57.79%), Postives = 121/154 (78.57%), Query Frame = 1


Query: 6   MGLDPIFLNTLHDLLDFTDDPG-QSSHHAPSRAYVRDAKAMAATPADVVEYPNSYQFTID 65
           +G  PI ++ L D+L+  +D   + + + PSR Y+RDAKAMAATPADV+E+PN+Y F +D
Sbjct: 3   LGRFPI-ISILEDMLEVPEDHNNEKTRNNPSRVYMRDAKAMAATPADVIEHPNAYAFVVD 62

Query: 66  MPGLKPDQIKVKIE-DNQLVVSGERKRESEKVKEGKFVRMERRLGKYLKKFDLPETADAE 125
           MPG+K D+IKV++E DN LVVSGER+RE+++ +  K+VRMERR+GK+++KF LPE AD +
Sbjct: 63  MPGIKGDEIKVQVENDNVLVVSGERQRENKENEGVKYVRMERRMGKFMRKFQLPENADLD 122

Query: 126 KVSAAYQDGVLSVTVEKKPPPEPKKAKNIEVHVA 158
           K+SA   DGVL VTV+K PPPEPKK K I+V VA
Sbjct: 123 KISAVCHDGVLKVTVQKLPPPEPKKPKTIQVQVA 155

[Close]

BLAST of Lsi04G014020.1 vs. TAIR10
Match: AT5G12030.1 (AT5G12030.1 heat shock protein 17.6A)

HSP 1 Score: 170.6 bits (431), Expect = 7.5e-43
Identity = 87/154 (56.49%), Postives = 122/154 (79.22%), Query Frame = 1


Query: 5   KMGLDPIFLNTLHDLLDFTDDPGQSSHHAPSRAYVRDAKAMAATPADVVEYPNSYQFTID 64
           + G  PIF + L D+L+  ++  + + + PSRAY+RDAKAMAATPADV+E+P++Y F +D
Sbjct: 4   EFGRFPIF-SILEDMLEAPEEQTEKTRNNPSRAYMRDAKAMAATPADVIEHPDAYVFAVD 63

Query: 65  MPGLKPDQIKVKIE-DNQLVVSGERKRESEKVKEGKFVRMERRLGKYLKKFDLPETADAE 124
           MPG+K D+I+V+IE +N LVVSG+R+R++++ +  KFVRMERR+GK+++KF LP+ AD E
Sbjct: 64  MPGIKGDEIQVQIENENVLVVSGKRQRDNKENEGVKFVRMERRMGKFMRKFQLPDNADLE 123

Query: 125 KVSAAYQDGVLSVTVEKKPPPEPKKAKNIEVHVA 158
           K+SAA  DGVL VT+ K PPPEPKK K I+V VA
Sbjct: 124 KISAACNDGVLKVTIPKLPPPEPKKPKTIQVQVA 156

[Close]

BLAST of Lsi04G014020.1 vs. TAIR10
Match: AT1G54050.1 (AT1G54050.1 HSP20-like chaperones superfamily protein)

HSP 1 Score: 90.5 bits (223), Expect = 9.9e-19
Identity = 50/114 (43.86%), Postives = 79/114 (69.30%), Query Frame = 1


Query: 49  PADVVEYPNSYQFTIDMPGLKPDQIKVKIEDNQLVV---SGERKRESEKVKEG-KFVRME 108
           P D++E P  Y F +D+PG+    I+V +E+ + +V   +G+RKR+ ++ +EG K++R+E
Sbjct: 43  PIDILESPKEYIFYLDIPGISKSDIQVTVEEERTLVIKSNGKRKRDDDESEEGSKYIRLE 102

Query: 109 RRLGKYL-KKFDLPETADAEKVSAAYQDGVLSVTVEKKPPPEPKKAKNIEVHVA 158
           RRL + L KKF LPE AD   V+A YQ+GVL+V + KK PP+P K K +++ V+
Sbjct: 103 RRLAQNLVKKFRLPEDADMASVTAKYQEGVLTVVI-KKLPPQPPKPKTVQIAVS 155

[Close]

BLAST of Lsi04G014020.1 vs. TAIR10
Match: AT5G59720.1 (AT5G59720.1 heat shock protein 18.2)

HSP 1 Score: 85.5 bits (210), Expect = 3.2e-17
Identity = 49/116 (42.24%), Postives = 76/116 (65.52%), Query Frame = 1


Query: 40  RDAKAMAATPADVVEYPNSYQFTIDMPGLKPDQIKVKIED-NQLVVSGERKRESEKVKEG 99
           RD  A      D  E P ++ F  D+PGLK +++KV++ED N L +SGER +E+E+ K  
Sbjct: 44  RDVAAFTNARVDWKETPEAHVFKADLPGLKKEEVKVEVEDKNVLQISGERSKENEE-KND 103

Query: 100 KFVRMERRLGKYLKKFDLPETADAEKVSAAYQDGVLSVTVEKKPPPEPKKAKNIEV 155
           K+ R+ER  GK++++F LPE A  E+V A  ++GVL+V V K P  +P + K+I++
Sbjct: 104 KWHRVERASGKFMRRFRLPENAKMEEVKATMENGVLTVVVPKAPEKKP-QVKSIDI 157

[Close]

BLAST of Lsi04G014020.1 vs. TAIR10
Match: AT1G53540.1 (AT1G53540.1 HSP20-like chaperones superfamily protein)

HSP 1 Score: 83.2 bits (204), Expect = 1.6e-16
Identity = 49/121 (40.50%), Postives = 77/121 (63.64%), Query Frame = 1


Query: 35  SRAYVRDAKAMAATPADVVEYPNSYQFTIDMPGLKPDQIKVKIED-NQLVVSGERKRESE 94
           + A   D  A      D  E P ++ F  D+PGL+ +++KV++ED N L +SGER  E+E
Sbjct: 37  ANAPAMDVAAFTNAKVDWRETPEAHVFKADLPGLRKEEVKVEVEDGNILQISGERSNENE 96

Query: 95  KVKEGKFVRMERRLGKYLKKFDLPETADAEKVSAAYQDGVLSVTVEKKPPPEPKKAKNIE 154
           + K  K+ R+ER  GK+ ++F LPE A  E++ A+ ++GVLSVTV K P  +P + K+I+
Sbjct: 97  E-KNDKWHRVERSSGKFTRRFRLPENAKMEEIKASMENGVLSVTVPKVPEKKP-EVKSID 155

[Close]

BLAST of Lsi04G014020.1 vs. NCBI nr
Match: gi|659103977|ref|XP_008452768.1| (PREDICTED: 17.3 kDa class II heat shock protein-like [Cucumis melo])

HSP 1 Score: 287.3 bits (734), Expect = 1.6e-74
Identity = 141/157 (89.81%), Postives = 151/157 (96.18%), Query Frame = 1


Query: 1   MEVAKMGLDPIFLNTLHDLLDFTDDPGQSSHHAPSRAYVRDAKAMAATPADVVEYPNSYQ 60
           M+VAKMG DP+FLN LHDLLDF+D+PGQSSHHAPSRAY+RDAKAMAATPADVVEYPNSYQ
Sbjct: 1   MDVAKMGFDPLFLNALHDLLDFSDEPGQSSHHAPSRAYLRDAKAMAATPADVVEYPNSYQ 60

Query: 61  FTIDMPGLKPDQIKVKIEDNQLVVSGERKRESEKVKEGKFVRMERRLGKYLKKFDLPETA 120
           FTIDMPGLK DQIKVK+ED QLVVSGERKRESEKVKEGKFVRMERRLGKYLKKF+LPETA
Sbjct: 61  FTIDMPGLKSDQIKVKVEDGQLVVSGERKRESEKVKEGKFVRMERRLGKYLKKFELPETA 120

Query: 121 DAEKVSAAYQDGVLSVTVEKKPPPEPKKAKNIEVHVA 158
           D +K+SAAYQDGVLSVTVEKKPPPEPKKAK IE+HVA
Sbjct: 121 DTDKISAAYQDGVLSVTVEKKPPPEPKKAKTIEIHVA 157

[Close]

BLAST of Lsi04G014020.1 vs. NCBI nr
Match: gi|449447157|ref|XP_004141335.1| (PREDICTED: 17.9 kDa class II heat shock protein [Cucumis sativus])

HSP 1 Score: 279.6 bits (714), Expect = 3.3e-72
Identity = 142/158 (89.87%), Postives = 150/158 (94.94%), Query Frame = 1


Query: 1   MEVAKM-GLDPIFLNTLHDLLDFTDDPGQSSHHAPSRAYVRDAKAMAATPADVVEYPNSY 60
           MEVAKM G DP+FLN LHDLLDF+D+PGQ SHHAPSRAY+RDAKAMAATPADVVEYPNSY
Sbjct: 1   MEVAKMPGFDPLFLNALHDLLDFSDEPGQGSHHAPSRAYLRDAKAMAATPADVVEYPNSY 60

Query: 61  QFTIDMPGLKPDQIKVKIEDNQLVVSGERKRESEKVKEGKFVRMERRLGKYLKKFDLPET 120
           QFTIDMPGL  DQIKVK+ED QLVVSGERKRESEKVKEGKFVRMERRLGKYLKKFDLPET
Sbjct: 61  QFTIDMPGLTSDQIKVKVEDGQLVVSGERKRESEKVKEGKFVRMERRLGKYLKKFDLPET 120

Query: 121 ADAEKVSAAYQDGVLSVTVEKKPPPEPKKAKNIEVHVA 158
           ADA+KVSAAY+DGVLSVTVEKKPPPEPKKAK+IEV VA
Sbjct: 121 ADADKVSAAYRDGVLSVTVEKKPPPEPKKAKSIEVRVA 158

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BLAST of Lsi04G014020.1 vs. NCBI nr
Match: gi|702291759|ref|XP_010047373.1| (PREDICTED: 17.1 kDa class II heat shock protein-like [Eucalyptus grandis])

HSP 1 Score: 219.9 bits (559), Expect = 3.1e-54
Identity = 114/168 (67.86%), Postives = 135/168 (80.36%), Query Frame = 1


Query: 1   MEVAKMGLDPIFLNTLHDLLDFTDDPGQSSHHAPSRAYVRDAKAMAATPADVVEYPNSYQ 60
           M++  MG DP  L+TLH+LLD +D+P   SHHAPSRA++RD KAMAATPADV EYPNSY 
Sbjct: 1   MDLRDMGFDPTVLDTLHELLDLSDEPDAKSHHAPSRAFLRDKKAMAATPADVKEYPNSYV 60

Query: 61  FTIDMPGLKPDQIKVKIED-NQLVVSGERKRESEK----VKEG------KFVRMERRLGK 120
           F +DMPGLKPDQIKV++ED N LVVSGERKRE EK     K+G      ++V+MERRLGK
Sbjct: 61  FVLDMPGLKPDQIKVQVEDGNMLVVSGERKREKEKDLKDQKDGAGGGLLRYVKMERRLGK 120

Query: 121 YLKKFDLPETADAEKVSAAYQDGVLSVTVEKKPPPEPKKAKNIEVHVA 158
           +LKKF LPE AD EK+SA+YQDGVL+VTVEK+PPPEPKK K IEV +A
Sbjct: 121 FLKKFVLPENADPEKISASYQDGVLTVTVEKRPPPEPKKPKTIEVQIA 168

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BLAST of Lsi04G014020.1 vs. NCBI nr
Match: gi|590574333|ref|XP_007012372.1| (Heat-shock protein, putative [Theobroma cacao])

HSP 1 Score: 206.5 bits (524), Expect = 3.5e-50
Identity = 107/158 (67.72%), Postives = 128/158 (81.01%), Query Frame = 1


Query: 1   MEVAKMGLDPIFLNTLHDLLDFTDDPGQSSHHAPSRAYVRDAKAMAATPADVVEYPNSYQ 60
           M+   MG DP  ++TLH+LLDF DD  +S  H PSRAY+RDAKAMAATPADV + PN+Y 
Sbjct: 1   MDWRVMGFDPSVIDTLHELLDFPDDADKSQPH-PSRAYIRDAKAMAATPADVKDCPNAYV 60

Query: 61  FTIDMPGLKPDQIKVKIED-NQLVVSGERKRESEKVKEGKFVRMERRLGKYLKKFDLPET 120
             IDMPGLK DQ+KV++E+ N LVVSGERKRE EK +  KF++MERRLGKYLKKF LPE 
Sbjct: 61  VVIDMPGLKQDQLKVQVEEGNFLVVSGERKREKEKDQGVKFIKMERRLGKYLKKFQLPEM 120

Query: 121 ADAEKVSAAYQDGVLSVTVEKKPPPEPKKAKNIEVHVA 158
           AD EK+SA+YQDGVL+VTVEKKPPPEPKK + +EV V+
Sbjct: 121 ADTEKISASYQDGVLTVTVEKKPPPEPKKPRTVEVQVS 157

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BLAST of Lsi04G014020.1 vs. NCBI nr
Match: gi|703082483|ref|XP_010091963.1| (hypothetical protein L484_003776 [Morus notabilis])

HSP 1 Score: 204.9 bits (520), Expect = 1.0e-49
Identity = 110/159 (69.18%), Postives = 129/159 (81.13%), Query Frame = 1


Query: 6   MGLDPIFLN--TLHDLLDFTDD----PGQSSHHAPSRAYVRDAKAMAATPADVVEYPNSY 65
           MGLDP  L   TLHD+LD++++    PG  +HHAPSRAYVRDAKAMAATPADV + PN+Y
Sbjct: 1   MGLDPAQLIEFTLHDILDYSEETSGGPGGEAHHAPSRAYVRDAKAMAATPADVKDTPNAY 60

Query: 66  QFTIDMPGLKPDQIKVKIED-NQLVVSGERKRESEKVKEGKFVRMERRLGKYLKKFDLPE 125
            F IDMPGLK DQIKV +E+ N LVVSGERKRE EK +  K++RMERRLGKYLKKF LPE
Sbjct: 61  VFVIDMPGLKQDQIKVHVEEENVLVVSGERKREREKDQGVKYLRMERRLGKYLKKFVLPE 120

Query: 126 TADAEKVSAAYQDGVLSVTVEKKPPPEPKKAKNIEVHVA 158
            AD +K+SA+YQDGVL+VT++KKPPPEPKK K  EV VA
Sbjct: 121 NADTDKISASYQDGVLTVTIQKKPPPEPKKPKTSEVQVA 159

The following BLAST results are available for this feature:

BLAST of Lsi04G014020.1 vs. Swiss-Prot


Match Name	E-value	Identity	Description
HSP22_IPONI	1.3e-44	60.00	18.8 kDa class II heat shock protein OS=Ipomoea nil GN=SHSP-2 PE=2 SV=1	[more]
HSP21_PEA	1.4e-43	61.69	17.1 kDa class II heat shock protein OS=Pisum sativum GN=HSP17.7 PE=2 SV=1	[more]
HSP21_SOLPE	1.9e-43	60.00	17.3 kDa class II heat shock protein OS=Solanum peruvianum PE=2 SV=1	[more]
HSP21_HELAN	1.9e-43	63.69	17.9 kDa class II heat shock protein OS=Helianthus annuus GN=HSP17.9 PE=2 SV=1	[more]
HS176_ARATH	6.0e-42	57.79	17.6 kDa class II heat shock protein OS=Arabidopsis thaliana GN=HSP17.6 PE=2 SV=...	[more]

BLAST of Lsi04G014020.1 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L5D4_CUCSA	2.3e-72	89.87	Uncharacterized protein OS=Cucumis sativus GN=Csa_4G646350 PE=3 SV=1	[more]
A0A059CLA5_EUCGR	2.1e-54	67.86	Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_C00678 PE=3 SV=1	[more]
A0A061GKC6_THECC	2.4e-50	67.72	Heat-shock protein, putative OS=Theobroma cacao GN=TCM_037346 PE=3 SV=1	[more]
W9QXD5_9ROSA	7.1e-50	69.18	Uncharacterized protein OS=Morus notabilis GN=L484_003776 PE=3 SV=1	[more]
B9RQT8_RICCO	2.1e-49	70.00	Heat-shock protein, putative OS=Ricinus communis GN=RCOM_0706500 PE=3 SV=1	[more]

BLAST of Lsi04G014020.1 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G12020.1	3.4e-43	57.79	17.6 kDa class II heat shock protein	[more]
AT5G12030.1	7.5e-43	56.49	heat shock protein 17.6A	[more]
AT1G54050.1	9.9e-19	43.86	HSP20-like chaperones superfamily protein	[more]
AT5G59720.1	3.2e-17	42.24	heat shock protein 18.2	[more]
AT1G53540.1	1.6e-16	40.50	HSP20-like chaperones superfamily protein	[more]

BLAST of Lsi04G014020.1 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659103977\|ref\|XP_008452768.1\|	1.6e-74	89.81	PREDICTED: 17.3 kDa class II heat shock protein-like [Cucumis melo]	[more]
gi\|449447157\|ref\|XP_004141335.1\|	3.3e-72	89.87	PREDICTED: 17.9 kDa class II heat shock protein [Cucumis sativus]	[more]
gi\|702291759\|ref\|XP_010047373.1\|	3.1e-54	67.86	PREDICTED: 17.1 kDa class II heat shock protein-like [Eucalyptus grandis]	[more]
gi\|590574333\|ref\|XP_007012372.1\|	3.5e-50	67.72	Heat-shock protein, putative [Theobroma cacao]	[more]
gi\|703082483\|ref\|XP_010091963.1\|	1.0e-49	69.18	hypothetical protein L484_003776 [Morus notabilis]	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **INTERPRO**
Term	Definition
IPR008978	HSP20-like_chaperone
IPR002068	A-crystallin/Hsp20_dom

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003674	molecular_function

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Lsi04G014020	Lsi04G014020	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lsi04G014020.1.CDS.1	Lsi04G014020.1.CDS.1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Lsi04G014020.1	Lsi04G014020.1-protein	polypeptide

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002068	Alpha crystallin/Hsp20 domain	PFAM	PF00011	HSP20	coord: 51..154 score: 2.1
IPR002068	Alpha crystallin/Hsp20 domain	PROFILE	PS01031	HSP20	coord: 51..140 score: 24
IPR008978	HSP20-like chaperone	GENE3D	G3DSA:2.60.40.790		coord: 14..155 score: 6.3
IPR008978	HSP20-like chaperone	unknown	SSF49764	HSP20-like chaperones	coord: 29..149 score: 3.49
None	No IPR available	PANTHER	PTHR11527:SF93	17.6 KDA CLASS II HEAT SHOCK PROTEIN-RELATED	coord: 6..157 score: 6.3