Lsi02G024460.1 (mRNA) Bottle gourd (USVL1VR-Ls)

NameLsi02G024460.1
TypemRNA
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls))
DescriptionMitochondrial carrier family protein
Locationchr02 : 31030217 .. 31030678 (-)
Sequence length462
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAGGCGTTTCAGTGAGCGTTTACAATGGCCTGAAGCGCTATTTGAGGAAAAAAAGCTACCGGAAACTGGCTGGGTCGGTGGGCCGTAGGACGGACACAGTGCAACTTGGGGCCACTGGAGGGAGGCGGCGGCGGCGGATTTGGCGGATCAAGATCTCGCCTAAGCTCAGGCTGTTCCGGCGCCTGCCGTCACTGAAGAAACTGCTTCTATGGCTGAGAGATTCGTATGTTAAGGCGATGTTGGCATTTGCCAACTCGAGGGTCATCGGCTCCGGCACTACCGGCGGCGGCGGCGATGGGATCTGGGGCAGCGCCGGAAGAAGCACGTTGAAGGAGTATGATGAGAAAATGATCACTGAGATCTACAAGTCGTTGGTGGTGGCGCAGGGACGGTTGCCGCCGCCGGAATCTGTCCGGCCGAGTTGGGTTGGCGGCCGGTTGCCGACGGTGGCGGAATAA

mRNA sequence

ATGGAAGGCGTTTCAGTGAGCGTTTACAATGGCCTGAAGCGCTATTTGAGGAAAAAAAGCTACCGGAAACTGGCTGGGTCGGTGGGCCGTAGGACGGACACAGTGCAACTTGGGGCCACTGGAGGGAGGCGGCGGCGGCGGATTTGGCGGATCAAGATCTCGCCTAAGCTCAGGCTGTTCCGGCGCCTGCCGTCACTGAAGAAACTGCTTCTATGGCTGAGAGATTCGTATGTTAAGGCGATGTTGGCATTTGCCAACTCGAGGGTCATCGGCTCCGGCACTACCGGCGGCGGCGGCGATGGGATCTGGGGCAGCGCCGGAAGAAGCACGTTGAAGGAGTATGATGAGAAAATGATCACTGAGATCTACAAGTCGTTGGTGGTGGCGCAGGGACGGTTGCCGCCGCCGGAATCTGTCCGGCCGAGTTGGGTTGGCGGCCGGTTGCCGACGGTGGCGGAATAA

Coding sequence (CDS)

ATGGAAGGCGTTTCAGTGAGCGTTTACAATGGCCTGAAGCGCTATTTGAGGAAAAAAAGCTACCGGAAACTGGCTGGGTCGGTGGGCCGTAGGACGGACACAGTGCAACTTGGGGCCACTGGAGGGAGGCGGCGGCGGCGGATTTGGCGGATCAAGATCTCGCCTAAGCTCAGGCTGTTCCGGCGCCTGCCGTCACTGAAGAAACTGCTTCTATGGCTGAGAGATTCGTATGTTAAGGCGATGTTGGCATTTGCCAACTCGAGGGTCATCGGCTCCGGCACTACCGGCGGCGGCGGCGATGGGATCTGGGGCAGCGCCGGAAGAAGCACGTTGAAGGAGTATGATGAGAAAATGATCACTGAGATCTACAAGTCGTTGGTGGTGGCGCAGGGACGGTTGCCGCCGCCGGAATCTGTCCGGCCGAGTTGGGTTGGCGGCCGGTTGCCGACGGTGGCGGAATAA

Protein sequence

MEGVSVSVYNGLKRYLRKKSYRKLAGSVGRRTDTVQLGATGGRRRRRIWRIKISPKLRLFRRLPSLKKLLLWLRDSYVKAMLAFANSRVIGSGTTGGGGDGIWGSAGRSTLKEYDEKMITEIYKSLVVAQGRLPPPESVRPSWVGGRLPTVAE
BLAST of Lsi02G024460.1 vs. TrEMBL
Match: A0A0A0K7Z4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G405950 PE=4 SV=1)

HSP 1 Score: 275.4 bits (703), Expect = 4.2e-71
Identity = 141/154 (91.56%), Postives = 146/154 (94.81%), Query Frame = 1

Query: 1   MEGVSVSVYNGLKRYLRKKSYRKLAGSVGRRTDTVQLGATGG-RRRRRIWRIKISPKLRL 60
           MEGVSVSVYNGL+RYLR+KSYRKLA SVGRRTDT QLGA GG RRRRRIWRIKISPKLRL
Sbjct: 1   MEGVSVSVYNGLRRYLRRKSYRKLAASVGRRTDTAQLGAAGGGRRRRRIWRIKISPKLRL 60

Query: 61  FRRLPSLKKLLLWLRDSYVKAMLAFANSRVIGSGTTGGGGDGIWGSAGRSTLKEYDEKMI 120
           FRR+PSLKKLLLWLRDSYVKAMLAFANSRVIGSG+  GGGDGIWG AGRSTLKEYDEKMI
Sbjct: 61  FRRMPSLKKLLLWLRDSYVKAMLAFANSRVIGSGSGSGGGDGIWGGAGRSTLKEYDEKMI 120

Query: 121 TEIYKSLVVAQGRLPPPESVRPSWVGGRLPTVAE 154
           TEIYKSLVVAQGRLPPPE+VRPSW GGRLPTVAE
Sbjct: 121 TEIYKSLVVAQGRLPPPETVRPSWGGGRLPTVAE 154

BLAST of Lsi02G024460.1 vs. TrEMBL
Match: A0A067K7J9_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_13986 PE=4 SV=1)

HSP 1 Score: 143.7 bits (361), Expect = 1.9e-31
Identity = 79/153 (51.63%), Postives = 112/153 (73.20%), Query Frame = 1

Query: 1   MEGVSVSVYNGLKRYLRKKSYRKLAGSVGRRTDTVQLGATGGRRRRRIWRIKISPKLRLF 60
           MEGVS ++Y  ++RY R++ Y +L  S  RR +TV+LG+T  +++RR+WRIKI PKL++F
Sbjct: 1   MEGVSTAMYTKVRRYWRRRGYERLNESTRRRQNTVELGSTL-QKKRRVWRIKIKPKLKIF 60

Query: 61  RRLPSLKKLLLWLRDSYVKAMLAFANSRVIGSGTTGGGGDGIWGSAGRSTLKEYDEKMIT 120
           + + S +K  +WLRD+YVK M+ FANSRVIG+G  GG GDG+  + G+  +KEYDE+MI 
Sbjct: 61  K-MSSPRKFFVWLRDAYVKMMMGFANSRVIGAGYGGGAGDGL-ATFGKRPVKEYDERMII 120

Query: 121 EIYKSLVVAQGRLPPPESVRPSWVGGRLPTVAE 154
           EIYKSL++AQG+L   E+ R   V  RLP + E
Sbjct: 121 EIYKSLMMAQGQLVAREAPRFGSV-PRLPAIVE 149

BLAST of Lsi02G024460.1 vs. TrEMBL
Match: V4U6A3_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10022679mg PE=4 SV=1)

HSP 1 Score: 143.3 bits (360), Expect = 2.5e-31
Identity = 82/143 (57.34%), Postives = 100/143 (69.93%), Query Frame = 1

Query: 1   MEGVSVSVYNGLKRYLRKKSYRKL-AGSVGRRTDTVQLGATGGRRRRRIWRIKISPKLRL 60
           MEGVS +VY GL+ Y R+K Y KL     GRRT   +LG+T  RRRR  WRIKI PKL++
Sbjct: 1   MEGVSTTVYKGLRGYWRRKGYEKLNREGRGRRTSRSELGSTSTRRRRFSWRIKIKPKLKI 60

Query: 61  FRRLPSLKKLLLWLRDSYVKAMLAFANSRVIGSGTTGGGGDGIWGS--AGRSTLKEYDEK 120
             RLPS KK  +WLRD+YVK M+ FANSRV  SG  GGG  G  G+   G+  LKEYDEK
Sbjct: 61  -SRLPSPKKFFIWLRDAYVKMMMGFANSRVCASGGYGGGAIGSNGAYGFGKGPLKEYDEK 120

Query: 121 MITEIYKSLVVAQGRLPPPESVR 141
           +I +IYKSLV+AQG+L P ++ R
Sbjct: 121 VIVDIYKSLVMAQGQLVPRDAAR 142

BLAST of Lsi02G024460.1 vs. TrEMBL
Match: A0A067H7Z2_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g031898mg PE=4 SV=1)

HSP 1 Score: 143.3 bits (360), Expect = 2.5e-31
Identity = 82/143 (57.34%), Postives = 100/143 (69.93%), Query Frame = 1

Query: 1   MEGVSVSVYNGLKRYLRKKSYRKL-AGSVGRRTDTVQLGATGGRRRRRIWRIKISPKLRL 60
           MEGVS +VY GL+ Y R+K Y KL     GRRT   +LG+T  RRRR  WRIKI PKL++
Sbjct: 1   MEGVSTTVYKGLRGYWRRKGYEKLNREGRGRRTSRSELGSTSTRRRRFSWRIKIKPKLKI 60

Query: 61  FRRLPSLKKLLLWLRDSYVKAMLAFANSRVIGSGTTGGGGDGIWGS--AGRSTLKEYDEK 120
             RLPS KK  +WLRD+YVK M+ FANSRV  SG  GGG  G  G+   G+  LKEYDEK
Sbjct: 61  -SRLPSPKKFFIWLRDAYVKMMMGFANSRVCASGGYGGGAIGSNGAYGFGKGPLKEYDEK 120

Query: 121 MITEIYKSLVVAQGRLPPPESVR 141
           +I +IYKSLV+AQG+L P ++ R
Sbjct: 121 VIVDIYKSLVMAQGQLVPRDAAR 142

BLAST of Lsi02G024460.1 vs. TrEMBL
Match: W9RL25_9ROSA (Uncharacterized protein OS=Morus notabilis GN=L484_007465 PE=4 SV=1)

HSP 1 Score: 131.3 bits (329), Expect = 9.8e-28
Identity = 76/141 (53.90%), Postives = 96/141 (68.09%), Query Frame = 1

Query: 1   MEGVSVSVYNGLKRYLRKKSYRKLAGSVGRRTDTVQLGATGGRRRRRIWRIKISPKLRLF 60
           MEGVS S+Y GL+ Y +++ Y +L GS  RR       +    R++R WRI+I+PKL   
Sbjct: 1   MEGVSESMYKGLRGYWKRRGYERLNGSGSRRRKR----SLDSIRKKRSWRIRIAPKLGFL 60

Query: 61  RRLPSLKKLLLWLRDSYVKAMLAFANSRVIGSGTTGG-GGDGIWGSAGRSTLKEYDEKMI 120
           RR+PS KKLL+WLRD+YVK ML FANSRV  +G      GDGI G  GR  LKEYD+KMI
Sbjct: 61  RRVPSPKKLLVWLRDAYVKLMLGFANSRVCSTGYGSAICGDGI-GGFGRGPLKEYDDKMI 120

Query: 121 TEIYKSLVVAQGRLPPPESVR 141
            EIYKSLV+A G+L P ++ R
Sbjct: 121 VEIYKSLVLAHGQLVPRDAAR 136

BLAST of Lsi02G024460.1 vs. TAIR10
Match: AT3G62650.2 (AT3G62650.2 unknown protein)

HSP 1 Score: 94.7 bits (234), Expect = 5.1e-20
Identity = 62/126 (49.21%), Postives = 79/126 (62.70%), Query Frame = 1

Query: 13  KRYLRK-KSYRKLAGSV----GRRTDT-VQLGATGGRRRRRIWRIKISPKLRLFRRLPSL 72
           KRY R+ + Y KL GS     GRR    V++  T   R++R WRIKI PKLR+  +  S 
Sbjct: 6   KRYWRRWRGYEKLDGSSETTSGRRKGKRVKMDPT---RKKRFWRIKIVPKLRIL-KTASP 65

Query: 73  KKLLLWLRDSYVKAMLAFANSRVIGSGTTGGGGDGIWGSAGRSTLKEYDEKMITEIYKSL 132
           KK L+WLRDSYVK M+  ANSRV+GS   GG G       G   +KEYDEKM+ EIYKS+
Sbjct: 66  KKFLVWLRDSYVKMMMRLANSRVVGSSGYGGSG------FGSGQMKEYDEKMLVEIYKSI 121

BLAST of Lsi02G024460.1 vs. TAIR10
Match: AT2G47485.1 (AT2G47485.1 unknown protein)

HSP 1 Score: 94.4 bits (233), Expect = 6.7e-20
Identity = 59/130 (45.38%), Postives = 76/130 (58.46%), Query Frame = 1

Query: 4   VSVSVYNGLKRYLRKKSYRKLAGSVGRRTDTVQLGATGGRRRRRIWRIKISPKLRLFRRL 63
           + +  +  L+ Y R     K A S  R    V++  T   R+RR WRIKI PKLR+ ++ 
Sbjct: 1   MEIRYWRRLRGYQRLDGSAKKASSGRRNVKRVKMDPT---RKRRFWRIKIVPKLRILKK- 60

Query: 64  PSLKKLLLWLRDSYVKAMLAFANSRVIGSGTTGGGGDGIWGSAGRSTLKEYDEKMITEIY 123
            S KKLL WLRD+YV  ML  ANSRV+GS      G G +G       KEYDEK + EIY
Sbjct: 61  ASPKKLLTWLRDAYVNMMLRLANSRVVGSSY----GYGEYGYGSGLASKEYDEKKLVEIY 120

Query: 124 KSLVVAQGRL 134
           KS+++AQG L
Sbjct: 121 KSILMAQGNL 122

BLAST of Lsi02G024460.1 vs. NCBI nr
Match: gi|449436451|ref|XP_004136006.1| (PREDICTED: uncharacterized protein LOC101203703 [Cucumis sativus])

HSP 1 Score: 275.4 bits (703), Expect = 6.0e-71
Identity = 141/154 (91.56%), Postives = 146/154 (94.81%), Query Frame = 1

Query: 1   MEGVSVSVYNGLKRYLRKKSYRKLAGSVGRRTDTVQLGATGG-RRRRRIWRIKISPKLRL 60
           MEGVSVSVYNGL+RYLR+KSYRKLA SVGRRTDT QLGA GG RRRRRIWRIKISPKLRL
Sbjct: 1   MEGVSVSVYNGLRRYLRRKSYRKLAASVGRRTDTAQLGAAGGGRRRRRIWRIKISPKLRL 60

Query: 61  FRRLPSLKKLLLWLRDSYVKAMLAFANSRVIGSGTTGGGGDGIWGSAGRSTLKEYDEKMI 120
           FRR+PSLKKLLLWLRDSYVKAMLAFANSRVIGSG+  GGGDGIWG AGRSTLKEYDEKMI
Sbjct: 61  FRRMPSLKKLLLWLRDSYVKAMLAFANSRVIGSGSGSGGGDGIWGGAGRSTLKEYDEKMI 120

Query: 121 TEIYKSLVVAQGRLPPPESVRPSWVGGRLPTVAE 154
           TEIYKSLVVAQGRLPPPE+VRPSW GGRLPTVAE
Sbjct: 121 TEIYKSLVVAQGRLPPPETVRPSWGGGRLPTVAE 154

BLAST of Lsi02G024460.1 vs. NCBI nr
Match: gi|659101268|ref|XP_008451518.1| (PREDICTED: uncharacterized protein LOC103492779 [Cucumis melo])

HSP 1 Score: 269.6 bits (688), Expect = 3.3e-69
Identity = 138/154 (89.61%), Postives = 143/154 (92.86%), Query Frame = 1

Query: 1   MEGVSVSVYNGLKRYLRKKSYRKLAGSVGRRTDTVQLGATGG-RRRRRIWRIKISPKLRL 60
           MEGVSVSVYNGL+RYLR+KSYRKLA SVG RT+T QLGA GG RRRRRIWRIKISPKLRL
Sbjct: 1   MEGVSVSVYNGLRRYLRRKSYRKLAASVGHRTETAQLGAAGGGRRRRRIWRIKISPKLRL 60

Query: 61  FRRLPSLKKLLLWLRDSYVKAMLAFANSRVIGSGTTGGGGDGIWGSAGRSTLKEYDEKMI 120
           FRR+PSLKKLLLWLRDSYVKAMLAFANSRVIG G+  GGGDGIWG AGRSTLKEYDEKMI
Sbjct: 61  FRRMPSLKKLLLWLRDSYVKAMLAFANSRVIGGGSGSGGGDGIWGGAGRSTLKEYDEKMI 120

Query: 121 TEIYKSLVVAQGRLPPPESVRPSWVGGRLPTVAE 154
           TEIYKSLVVAQGRLPPPE VRPSW GGRLPTVAE
Sbjct: 121 TEIYKSLVVAQGRLPPPEPVRPSWGGGRLPTVAE 154

BLAST of Lsi02G024460.1 vs. NCBI nr
Match: gi|802689209|ref|XP_012082843.1| (PREDICTED: uncharacterized protein LOC105642581 [Jatropha curcas])

HSP 1 Score: 143.7 bits (361), Expect = 2.7e-31
Identity = 79/153 (51.63%), Postives = 112/153 (73.20%), Query Frame = 1

Query: 1   MEGVSVSVYNGLKRYLRKKSYRKLAGSVGRRTDTVQLGATGGRRRRRIWRIKISPKLRLF 60
           MEGVS ++Y  ++RY R++ Y +L  S  RR +TV+LG+T  +++RR+WRIKI PKL++F
Sbjct: 1   MEGVSTAMYTKVRRYWRRRGYERLNESTRRRQNTVELGSTL-QKKRRVWRIKIKPKLKIF 60

Query: 61  RRLPSLKKLLLWLRDSYVKAMLAFANSRVIGSGTTGGGGDGIWGSAGRSTLKEYDEKMIT 120
           + + S +K  +WLRD+YVK M+ FANSRVIG+G  GG GDG+  + G+  +KEYDE+MI 
Sbjct: 61  K-MSSPRKFFVWLRDAYVKMMMGFANSRVIGAGYGGGAGDGL-ATFGKRPVKEYDERMII 120

Query: 121 EIYKSLVVAQGRLPPPESVRPSWVGGRLPTVAE 154
           EIYKSL++AQG+L   E+ R   V  RLP + E
Sbjct: 121 EIYKSLMMAQGQLVAREAPRFGSV-PRLPAIVE 149

BLAST of Lsi02G024460.1 vs. NCBI nr
Match: gi|1009148412|ref|XP_015891923.1| (PREDICTED: uncharacterized protein LOC107426304 [Ziziphus jujuba])

HSP 1 Score: 143.3 bits (360), Expect = 3.6e-31
Identity = 78/140 (55.71%), Postives = 99/140 (70.71%), Query Frame = 1

Query: 1   MEGVSVSVYNGLKRYLRKKSYRKLAGSVGRRTDTVQLGATGGRRRRRIWRIKISPKLRLF 60
           MEGVS SVY GL+ Y  ++ Y KL G   RR   V+LG+T  +RR+R+WRIK++PKL   
Sbjct: 46  MEGVSASVYKGLRGYWTRRGYEKLNGLDRRRKSRVELGST--QRRKRVWRIKVAPKLGFI 105

Query: 61  RRLPSLKKLLLWLRDSYVKAMLAFANSRVIGSGTTGGGGDGIWGSAGRSTLKEYDEKMIT 120
           RR+PS KKLL+WLRD+YVK ML FANSRV  S   G   +   G+ G+  LKEYDEKMI 
Sbjct: 106 RRMPSPKKLLVWLRDAYVKLMLGFANSRVFSSAYGGVICNDGTGAFGKGPLKEYDEKMIV 165

Query: 121 EIYKSLVVAQGRLPPPESVR 141
           EIYKSL +AQG+L P ++ +
Sbjct: 166 EIYKSLALAQGQLVPRDAAK 183

BLAST of Lsi02G024460.1 vs. NCBI nr
Match: gi|567903906|ref|XP_006444441.1| (hypothetical protein CICLE_v10022679mg [Citrus clementina])

HSP 1 Score: 143.3 bits (360), Expect = 3.6e-31
Identity = 82/143 (57.34%), Postives = 100/143 (69.93%), Query Frame = 1

Query: 1   MEGVSVSVYNGLKRYLRKKSYRKL-AGSVGRRTDTVQLGATGGRRRRRIWRIKISPKLRL 60
           MEGVS +VY GL+ Y R+K Y KL     GRRT   +LG+T  RRRR  WRIKI PKL++
Sbjct: 1   MEGVSTTVYKGLRGYWRRKGYEKLNREGRGRRTSRSELGSTSTRRRRFSWRIKIKPKLKI 60

Query: 61  FRRLPSLKKLLLWLRDSYVKAMLAFANSRVIGSGTTGGGGDGIWGS--AGRSTLKEYDEK 120
             RLPS KK  +WLRD+YVK M+ FANSRV  SG  GGG  G  G+   G+  LKEYDEK
Sbjct: 61  -SRLPSPKKFFIWLRDAYVKMMMGFANSRVCASGGYGGGAIGSNGAYGFGKGPLKEYDEK 120

Query: 121 MITEIYKSLVVAQGRLPPPESVR 141
           +I +IYKSLV+AQG+L P ++ R
Sbjct: 121 VIVDIYKSLVMAQGQLVPRDAAR 142

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A0A0K7Z4_CUCSA4.2e-7191.56Uncharacterized protein OS=Cucumis sativus GN=Csa_7G405950 PE=4 SV=1[more]
A0A067K7J9_JATCU1.9e-3151.63Uncharacterized protein OS=Jatropha curcas GN=JCGZ_13986 PE=4 SV=1[more]
V4U6A3_9ROSI2.5e-3157.34Uncharacterized protein OS=Citrus clementina GN=CICLE_v10022679mg PE=4 SV=1[more]
A0A067H7Z2_CITSI2.5e-3157.34Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g031898mg PE=4 SV=1[more]
W9RL25_9ROSA9.8e-2853.90Uncharacterized protein OS=Morus notabilis GN=L484_007465 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT3G62650.25.1e-2049.21 unknown protein[more]
AT2G47485.16.7e-2045.38 unknown protein[more]
Match NameE-valueIdentityDescription
gi|449436451|ref|XP_004136006.1|6.0e-7191.56PREDICTED: uncharacterized protein LOC101203703 [Cucumis sativus][more]
gi|659101268|ref|XP_008451518.1|3.3e-6989.61PREDICTED: uncharacterized protein LOC103492779 [Cucumis melo][more]
gi|802689209|ref|XP_012082843.1|2.7e-3151.63PREDICTED: uncharacterized protein LOC105642581 [Jatropha curcas][more]
gi|1009148412|ref|XP_015891923.1|3.6e-3155.71PREDICTED: uncharacterized protein LOC107426304 [Ziziphus jujuba][more]
gi|567903906|ref|XP_006444441.1|3.6e-3157.34hypothetical protein CICLE_v10022679mg [Citrus clementina][more]
The following terms have been associated with this mRNA:
Vocabulary: INTERPRO
TermDefinition
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0003674 molecular_function

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Lsi02G024460Lsi02G024460gene


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Lsi02G024460.1.CDS.1Lsi02G024460.1.CDS.1CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Lsi02G024460.1Lsi02G024460.1-proteinpolypeptide


Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePANTHERPTHR33702FAMILY NOT NAMEDcoord: 1..140
score: 4.2
NoneNo IPR availablePANTHERPTHR33702:SF5SUBFAMILY NOT NAMEDcoord: 1..140
score: 4.2