Lsi02G011250.1 (mRNA) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi02G011250.1
Type	mRNA
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Thymidine kinase
Location	chr02 : 13150789 .. 13154523 (+)
Sequence length	1533

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CATCCATAAAAAAAAAAAAAAAACGGAGTCCCCATTCACTCTCCAAAGTGCAAACCAAGAACCCTCGATGTTCTACAAATTGCAGACATTTCTCATCTGCAAAGCCAAAACCCCATTAAAACCCTCAACCAAAATCTCATGTTAACCATTTCAAAGATGAAATCCTTGGTATCTTCGTCTTCTTTCTCAACCCTCTCGCCCTATTTCCCCATTACCGCTTCTCCAACCTTCTTTTCTTTGCCTTCTAAATCTACGCAATGCAGCCCTATATTCACCCAAGTCTCCAATTACTTCACTTTCAAAACACCCAGAATTTTGTTTCCCTCAAAAGGGATTTCTTCAGCCCAGAATCGAACAGGGCAAATCGCAGCCACTTTGTCACCGCCCTCCGGCGAGATTCATGTGATTGTGGGCCCGATGTTTGCTGGGAAGACAACCACTCTTCTTCGGCGGATTCAGTCCGAGAGCAGCAATGGCAGGTCTTTTATGCTCTGCTTTTATGCTTCCGTTTATTAATCGAATGGGTTGCGAGCGATTAGTGTAGTGTTTTTTTCTAGCCTTATTTCAACTTGTTTCCCTTATATTTTTTAACTTTTTGCAACTAATGGAAACTGGGTTTTATACTTATGACAATAGATAGTGGATTTGCTGTTAGATTGGGAGAATTTTGTTTATCTTTTAATGTATTCTCAGCAATCTCTCTGCATTGGCTTGAATATTGGCATGATGGTATCAATATGAAATGGGAAGAAAATGGCATTGAATTTACAACCTCTTGTGCTATATTTACTTAAATCGAGACTAATATTGAGTGTTTGGGTGGATAAGTTATGGTAAATTTGGTGTTTTATTCTCTATCCCTACAAATTCTCAATAATGGAGTTATGTATATTGGTAATGGATGAGGCTGAAGCTGATATATGTTGTGGGTTATGGCCTCTTGATAGAGAATGAATTAAACGAGTGAATTGGTGAACTAAATTTAGCGATTCTTTGAAGAAAAGCTTTAGAAAAAGAACAAAAAGAAAAAGAAAAAAAATTTGAATCCCAAACTTTCCCTTGCTCCCTTAGCTCTTTTAAAAGATTCTTTCATTTTTTTAGCTCAACACTAGAAGATAGCCTTATTAACATAAGACCTCATGGCTTGGGTTTTTCTTTCAAAGAAGACTGCTAATATGGAAACATTGTTGCTCCAAGTTAGGAAGAGGAAATTTCACACCAAAGGTCTGGGGACAGTTTTCACTCAATCTATAAAATATGGGGACATTTAAGAAGTCAGTAACTTGCATTTACACCATGCTTTCTGCAGAGCATGCACCAAATCAATCTACTATTTGGAGTTAGATCACATGGTTTCAAAATGATACAACTACAAGAGTTCCAATGAGATTTGTGAAATAAAGGCTTAGGAGAACATGTTCAAGTCATAGTGGCCCCCTATCCAAAATTTAATATCATACGAGTTTCTCTGACAATTAAATGTAGTAGAACCAGCAGATTGCTGTCTTGTGAGAATTGTTGAAAGCGCTCAAGTTAGCTGGGATGCTCTGAAAAGAAAAAAGTTGTAGAGTTTTTGATAGATTTAATATTTCTATGCTTTTCTTAGTCTATGCTCAGGTTCTGGATATTGGATTAAGTGCACCATTTCAATTTTTGTGGAATCGGATTATCATATTCAAGACCCTCGATTTCAGCTTTTAATTAATTAGATTTTTTTATTTTTTATTTTTTATTTCCTTATTATTGTGTGTGTGTGTGGGGGGGGGGGGGGGGGGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGGGGGGGGGGGGGGGGGGGGGGGGACATCTTGTCTTCCTTTTGTGTCTTTATATTAAAAAAGATTGCCCTACCTTCGTACATTTGAGGTCAAGACAGCATCTTTCCTGCTGAAGGCTTATGATCTATATGCTTGTTGTTGATTGGATATAGTATAAATTTTTGGAAATTTTAGAACCTAATTACTTGGATCAGTTGGATGGAATGTATATTCTGATGTATCTTGATTGATTTAGAAGCTTCTTTTTTCAATACAATTCATAAGAATTATTCTTTCTTCTTTTCATGTATGTTTTTTTAACAGAAGTGTAGCTATAATTAAGTCAAATAAAGACACGAGATATGGATTGGATTCTATTGTGACACATGACGGCATGAAACTGCCTTGCTGGGCATTACCAAACTTAACATCTTTCAAACAGAAATTTGGTCAAGGTGCTTATGACAAGGTATCATAATCCCCCTCTCTTTGTGATATGCCAATGTACTTAAATTCTTCATATCTTGCTTAGACATTTCATTAATTAAATACAAAGTATTCTCATCCTTGTTAAAGGGAGCAAAGTATTTAAATCTATGAGGCTAAAGATTTGGTTTCTAATTAGAATTAGCAGACTGCAGAGGAGACTGATTGAGTAGCCTTCTGAATTTCGGTCACCTTCATAATTGGAATTCCTGATTTTCCTGGTAACAAAATTGTTAATTTAATTATGATATTGGTATGACAGCTAGATGTGATTGGAATTGATGAAGCTCAATTCTTCGACGATCTTTATGATTTCTGTCGTGAAGCTGCTGATATTGATGGCAAAACAGTTATAGTTGCTGGGCTGGATGGGGATTACTTGAGGTATAATCAAATTTACTGGCCTAGTTTTGGTTCATTCGCTAAAATAATTTCAATGAAACTGATGGCCTTTGGCTTTCATATGGTAAAACATTCAATAGAACTTCAGATTGGTTTAAATTGAATACTCATTAGTAACTCAGTTAAAGCTTTTGTGTTGGGGTTTATACAAAAAACTGAACAGGAGGAACTTCGGTTCAGTTCTCAATATAATTCCACTTGCTGATTCCGTAACAAAGTTAACTGCTCGATGTGAGATCTGCGGCAATCGGGCTTTCTTTACATTAAGGAAGACAGAAGAAAAAGAGACCGAGCTGATCGGTGGTGCTGACGTGTATATGCCCGTATGTCGACAACATTACGTCAGTGGGCAAGTAGTGATAGAGACAGCAAGAACTGTAGTAGAATCACACAAGCTTGAGTGTAGGACTCCAGCATAGTTGTTCTTAGTAGGTAGTATCAGGTTTGCAGCATCACATACATCCCCAGTAGCGAACGTCGCAACGTATTAGTTGTCCCGACGGGCGATTTTGGAACTATGATGAGCATAGTTGTAGTATTAGGGATATTTACTCAGAGATTTGAAGGCAAAAATATAGAAGGAAGGTGAAAAGGGGGTTTTGTTGTTGGTTTAGAAAATGGAATTGAGTTGGTTGTGCTTGTATTTTCATGGCTGCTTTGATTGATTGGATTAGATTAGAAGGAAAAATGAGTCAATGTTGTGTCATATGTAAGTATTTGTGTTGAATGAAAGAGGAGATGTAACCAACAAAGTTGAAAATTAATACATTATTGAGGGAGGAGACATTCAAGTGGCAACCATGTGTTTGCTTCGGCAGTTGTTGATCAGCCAGCTGTTGATTTTATTGATTGTAATTTCCATTGCCATATAACAACTACTTTTCATAATTAAAGACATGAAAAGTTGTTAATAATTGAATTAGAGCAAATATTATCCTTTTGAAATTATT

mRNA sequence

CATCCATAAAAAAAAAAAAAAAACGGAGTCCCCATTCACTCTCCAAAGTGCAAACCAAGAACCCTCGATGTTCTACAAATTGCAGACATTTCTCATCTGCAAAGCCAAAACCCCATTAAAACCCTCAACCAAAATCTCATGTTAACCATTTCAAAGATGAAATCCTTGGTATCTTCGTCTTCTTTCTCAACCCTCTCGCCCTATTTCCCCATTACCGCTTCTCCAACCTTCTTTTCTTTGCCTTCTAAATCTACGCAATGCAGCCCTATATTCACCCAAGTCTCCAATTACTTCACTTTCAAAACACCCAGAATTTTGTTTCCCTCAAAAGGGATTTCTTCAGCCCAGAATCGAACAGGGCAAATCGCAGCCACTTTGTCACCGCCCTCCGGCGAGATTCATGTGATTGTGGGCCCGATGTTTGCTGGGAAGACAACCACTCTTCTTCGGCGGATTCAGTCCGAGAGCAGCAATGGCAGAAGTGTAGCTATAATTAAGTCAAATAAAGACACGAGATATGGATTGGATTCTATTGTGACACATGACGGCATGAAACTGCCTTGCTGGGCATTACCAAACTTAACATCTTTCAAACAGAAATTTGGTCAAGGTGCTTATGACAAGCTAGATGTGATTGGAATTGATGAAGCTCAATTCTTCGACGATCTTTATGATTTCTGTCGTGAAGCTGCTGATATTGATGGCAAAACAGTTATAGTTGCTGGGCTGGATGGGGATTACTTGAGGAGGAACTTCGGTTCAGTTCTCAATATAATTCCACTTGCTGATTCCGTAACAAAGTTAACTGCTCGATGTGAGATCTGCGGCAATCGGGCTTTCTTTACATTAAGGAAGACAGAAGAAAAAGAGACCGAGCTGATCGGTGGTGCTGACGTGTATATGCCCGTATGTCGACAACATTACGTCAGTGGGCAAGTAGTGATAGAGACAGCAAGAACTGTAGTAGAATCACACAAGCTTGAGTGTAGGACTCCAGCATAGTTGTTCTTAGTAGGTAGTATCAGGTTTGCAGCATCACATACATCCCCAGTAGCGAACGTCGCAACGTATTAGTTGTCCCGACGGGCGATTTTGGAACTATGATGAGCATAGTTGTAGTATTAGGGATATTTACTCAGAGATTTGAAGGCAAAAATATAGAAGGAAGGTGAAAAGGGGGTTTTGTTGTTGGTTTAGAAAATGGAATTGAGTTGGTTGTGCTTGTATTTTCATGGCTGCTTTGATTGATTGGATTAGATTAGAAGGAAAAATGAGTCAATGTTGTGTCATATGTAAGTATTTGTGTTGAATGAAAGAGGAGATGTAACCAACAAAGTTGAAAATTAATACATTATTGAGGGAGGAGACATTCAAGTGGCAACCATGTGTTTGCTTCGGCAGTTGTTGATCAGCCAGCTGTTGATTTTATTGATTGTAATTTCCATTGCCATATAACAACTACTTTTCATAATTAAAGACATGAAAAGTTGTTAATAATTGAATTAGAGCAAATATTATCCTTTTGAAATTATT

Coding sequence (CDS)

ATGTTAACCATTTCAAAGATGAAATCCTTGGTATCTTCGTCTTCTTTCTCAACCCTCTCGCCCTATTTCCCCATTACCGCTTCTCCAACCTTCTTTTCTTTGCCTTCTAAATCTACGCAATGCAGCCCTATATTCACCCAAGTCTCCAATTACTTCACTTTCAAAACACCCAGAATTTTGTTTCCCTCAAAAGGGATTTCTTCAGCCCAGAATCGAACAGGGCAAATCGCAGCCACTTTGTCACCGCCCTCCGGCGAGATTCATGTGATTGTGGGCCCGATGTTTGCTGGGAAGACAACCACTCTTCTTCGGCGGATTCAGTCCGAGAGCAGCAATGGCAGAAGTGTAGCTATAATTAAGTCAAATAAAGACACGAGATATGGATTGGATTCTATTGTGACACATGACGGCATGAAACTGCCTTGCTGGGCATTACCAAACTTAACATCTTTCAAACAGAAATTTGGTCAAGGTGCTTATGACAAGCTAGATGTGATTGGAATTGATGAAGCTCAATTCTTCGACGATCTTTATGATTTCTGTCGTGAAGCTGCTGATATTGATGGCAAAACAGTTATAGTTGCTGGGCTGGATGGGGATTACTTGAGGAGGAACTTCGGTTCAGTTCTCAATATAATTCCACTTGCTGATTCCGTAACAAAGTTAACTGCTCGATGTGAGATCTGCGGCAATCGGGCTTTCTTTACATTAAGGAAGACAGAAGAAAAAGAGACCGAGCTGATCGGTGGTGCTGACGTGTATATGCCCGTATGTCGACAACATTACGTCAGTGGGCAAGTAGTGATAGAGACAGCAAGAACTGTAGTAGAATCACACAAGCTTGAGTGTAGGACTCCAGCATAG

Protein sequence

MLTISKMKSLVSSSSFSTLSPYFPITASPTFFSLPSKSTQCSPIFTQVSNYFTFKTPRILFPSKGISSAQNRTGQIAATLSPPSGEIHVIVGPMFAGKTTTLLRRIQSESSNGRSVAIIKSNKDTRYGLDSIVTHDGMKLPCWALPNLTSFKQKFGQGAYDKLDVIGIDEAQFFDDLYDFCREAADIDGKTVIVAGLDGDYLRRNFGSVLNIIPLADSVTKLTARCEICGNRAFFTLRKTEEKETELIGGADVYMPVCRQHYVSGQVVIETARTVVESHKLECRTPA

Homology

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BLAST of Lsi02G011250.1 vs. Swiss-Prot
Match: KITH_ORYSJ (Thymidine kinase OS=Oryza sativa subsp. japonica GN=TK PE=2 SV=2)

HSP 1 Score: 317.4 bits (812), Expect = 1.6e-85
Identity = 158/227 (69.60%), Postives = 185/227 (81.50%), Query Frame = 1


Query: 62  PSKGISSA-----QNRTGQIAATLSPPS--GEIHVIVGPMFAGKTTTLLRRIQSESSNG- 121
           P + +S+A     ++R G  +A  + PS  GEIHVIVGPMFAGKTT LLRR+Q E+  G 
Sbjct: 37  PVRPVSAAAAAADKSRGGGGSAMEAQPSYPGEIHVIVGPMFAGKTTALLRRVQVEAGTGS 96

Query: 122 RSVAIIKSNKDTRYGLDSIVTHDGMKLPCWALPNLTSFKQKFGQGAYDKLDVIGIDEAQF 181
           R+VA+IKS+KD RYGLDS+VTHDG K+PCWALP L+SF+ K G  AYDK+DVIGIDEAQF
Sbjct: 97  RNVALIKSDKDNRYGLDSVVTHDGTKMPCWALPELSSFQDKLGTEAYDKVDVIGIDEAQF 156

Query: 182 FDDLYDFCREAADIDGKTVIVAGLDGDYLRRNFGSVLNIIPLADSVTKLTARCEICGNRA 241
           FDDL+DFC +AAD DGK V+VAGLDGDY R  FGSVL+IIPLADSVTKLTARCE+CG RA
Sbjct: 157 FDDLHDFCCKAADRDGKIVVVAGLDGDYKRNKFGSVLDIIPLADSVTKLTARCELCGRRA 216

Query: 242 FFTLRKTEEKETELIGGADVYMPVCRQHYVSGQVVIETARTVVESHK 281
           FFTLRKT E +TELIGGADVYMPVCRQHY+ GQ+VIE  R V++  K
Sbjct: 217 FFTLRKTRETKTELIGGADVYMPVCRQHYLDGQIVIEATRIVLDLEK 263

[Close]

BLAST of Lsi02G011250.1 vs. Swiss-Prot
Match: KITHB_ARATH (Thymidine kinase b OS=Arabidopsis thaliana GN=TK1B PE=1 SV=1)

HSP 1 Score: 308.9 bits (790), Expect = 5.7e-83
Identity = 163/274 (59.49%), Postives = 208/274 (75.91%), Query Frame = 1


Query: 7   MKSLVSSSSFSTLSPYFPITASPTFFSLPSK-STQCSPIFTQVSNYFTFKTPRILFPSKG 66
           M++L+S S    L+P+      P+ FS   + S   + I    S   T  T ++   +  
Sbjct: 6   MRTLISPS----LAPFSLHLHKPSLFSTALRFSFSINNITPTNSPPSTISTRKLQTKATR 65

Query: 67  ISSAQNRTGQIAATLSPPSGEIHVIVGPMFAGKTTTLLRRIQSESSNGRSVAIIKSNKDT 126
           ++S+ +   Q  ++ SP  GEIHV+VGPMF+GKTTTLLRRI +E   G+ +AIIKSNKDT
Sbjct: 66  VTSSSS--SQPLSSSSP--GEIHVVVGPMFSGKTTTLLRRILAERETGKRIAIIKSNKDT 125

Query: 127 RYGLDSIVTHDGMKLPCWALPNLTSFKQKFGQGAYD-KLDVIGIDEAQFFDDLYDFCREA 186
           RY  +SIVTHDG K PCW+LP+L+SFK++FG   Y+ +LDVIGIDEAQFF DLY+FCREA
Sbjct: 126 RYCTESIVTHDGEKYPCWSLPDLSSFKERFGFDDYENRLDVIGIDEAQFFGDLYEFCREA 185

Query: 187 ADIDGKTVIVAGLDGDYLRRNFGSVLNIIPLADSVTKLTARCEICGNRAFFTLRKTEEKE 246
           AD +GKTVIVAGLDGD++RR FGSVL++IP+AD+VTKLT+RCE+CG RA FT+RKTEEKE
Sbjct: 186 ADKEGKTVIVAGLDGDFMRRRFGSVLDLIPIADTVTKLTSRCEVCGKRALFTMRKTEEKE 245

Query: 247 TELIGGADVYMPVCRQHYVSGQVVIETARTVVES 279
           TELIGGA+VYMPVCR HYV GQ V+ETAR V++S
Sbjct: 246 TELIGGAEVYMPVCRSHYVCGQNVLETARAVLDS 271

[Close]

BLAST of Lsi02G011250.1 vs. Swiss-Prot
Match: KITHA_ARATH (Thymidine kinase a OS=Arabidopsis thaliana GN=TK1A PE=1 SV=1)

HSP 1 Score: 300.1 bits (767), Expect = 2.7e-80
Identity = 138/194 (71.13%), Postives = 171/194 (88.14%), Query Frame = 1


Query: 84  SGEIHVIVGPMFAGKTTTLLRRIQSESSNGRSVAIIKSNKDTRYGLDSIVTHDGMKLPCW 143
           SG +HVI+GPMF+GK+T+LLRRI+SE S+GRSVA++KS+KDTRY  DS+VTHDG+  PCW
Sbjct: 30  SGAVHVIMGPMFSGKSTSLLRRIKSEISDGRSVAMLKSSKDTRYAKDSVVTHDGIGFPCW 89

Query: 144 ALPNLTSFKQKFGQGAYDKLDVIGIDEAQFFDDLYDFCREAADIDGKTVIVAGLDGDYLR 203
           ALP+L SF +KFG  AY+KLDVIGIDEAQFF DLY+FC + AD DGK VIVAGLDGDYLR
Sbjct: 90  ALPDLMSFPEKFGLDAYNKLDVIGIDEAQFFGDLYEFCCKVADDDGKIVIVAGLDGDYLR 149

Query: 204 RNFGSVLNIIPLADSVTKLTARCEICGNRAFFTLRKTEEKETELIGGADVYMPVCRQHYV 263
           R+FG+VL+IIP+ADSVTKLTARCE+CG++AFFTLRK  +  TELIGGADVYMPVCR+HY+
Sbjct: 150 RSFGAVLDIIPIADSVTKLTARCEVCGHKAFFTLRKNCDTRTELIGGADVYMPVCRKHYI 209

Query: 264 SGQVVIETARTVVE 278
           +  +VI+ ++ V+E
Sbjct: 210 TNHIVIKASKKVLE 223

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BLAST of Lsi02G011250.1 vs. Swiss-Prot
Match: KITH_SWPVK (Thymidine kinase OS=Swinepox virus (strain Kasza) GN=TK PE=3 SV=1)

HSP 1 Score: 161.0 bits (406), Expect = 1.9e-38
Identity = 81/180 (45.00%), Postives = 115/180 (63.89%), Query Frame = 1


Query: 85  GEIHVIVGPMFAGKTTTLLRRIQSESSNGRSVAIIKSNKDTRYGLDSIVTHDGMKLPCWA 144
           G IH+I+GPMF+GK+T L+R +        +  +IK +KD RYG D++ THD   +   +
Sbjct: 4   GFIHLILGPMFSGKSTELIRLVNRYQIATYNCRVIKYSKDNRYGNDAVYTHDKCYISAVS 63

Query: 145 LPNLTSFKQKFGQGAYDKLDVIGIDEAQFFDDLYDFCREAADIDGKTVIVAGLDGDYLRR 204
             +L   K        D +D++GIDE QFF+D+ +FC   A+  GK VIVA LDG Y R+
Sbjct: 64  TDSLFDIKDTL-----DDVDIVGIDEGQFFNDIVEFCEYIAN-KGKIVIVAALDGTYERK 123

Query: 205 NFGSVLNIIPLADSVTKLTARCEICGNRAFFTLRKTEEKETELIGGADVYMPVCRQHYVS 264
            FG++LN+IPL++ VTKL A C IC   A F+ R ++EKE ELIGG + Y+ VCR  Y++
Sbjct: 124 PFGNILNLIPLSEKVTKLNAICMICHRDASFSKRLSDEKEIELIGGKEKYLSVCRSCYLT 177

[Close]

BLAST of Lsi02G011250.1 vs. Swiss-Prot
Match: KITH_CHICK (Thymidine kinase, cytosolic OS=Gallus gallus GN=TK1 PE=1 SV=1)

HSP 1 Score: 150.2 bits (378), Expect = 3.4e-35
Identity = 83/183 (45.36%), Postives = 111/183 (60.66%), Query Frame = 1


Query: 83  PSGEIHVIVGPMFAGKTTTLLRRIQSESSNGRSVAIIKSNKDTRYGLDSIVTHDGMKL-- 142
           P G+I VI GPMF+GK+T L+RR++          ++K  KDTRY    + THD   +  
Sbjct: 17  PRGQIQVIFGPMFSGKSTELMRRVRRFQLAQYRCLLVKYAKDTRYCTTGVSTHDRNTMEA 76

Query: 143 -PCWALPNLTSFKQKFGQGAYDKLDVIGIDEAQFFDDLYDFCREAADIDGKTVIVAGLDG 202
            P  AL ++  +++  G        VIGIDE QFF D+ +FC + A+  GKTVIVA LDG
Sbjct: 77  RPACALQDV--YQEALGSA------VIGIDEGQFFPDIVEFCEKMANT-GKTVIVAALDG 136

Query: 203 DYLRRNFGSVLNIIPLADSVTKLTARCEICGNRAFFTLRKTEEKETELIGGADVYMPVCR 262
            + R+ FGS+LN++PLA+SV KL A C  C   A +T R   E+E E+IGGAD Y  VCR
Sbjct: 137 TFQRKAFGSILNLVPLAESVVKLNAVCMECYREASYTKRLGAEREVEVIGGADKYHSVCR 190

[Close]

BLAST of Lsi02G011250.1 vs. TrEMBL
Match: A0A0A0LYL4_CUCSA (Thymidine kinase OS=Cucumis sativus GN=Csa_1G181480 PE=3 SV=1)

HSP 1 Score: 500.4 bits (1287), Expect = 1.5e-138
Identity = 248/285 (87.02%), Postives = 264/285 (92.63%), Query Frame = 1


Query: 3   TISKMKSLVSSSSFSTLSPYFPITASPTFFSLPSKSTQCSPIFTQVSNYFTFKTPRILFP 62
           TIS MKS V SSSFS LSPYFPI+ASP+FFSLPSKS QC P+FTQ+SN+FTFKTP I  P
Sbjct: 69  TISNMKSFVFSSSFSILSPYFPISASPSFFSLPSKSAQCGPMFTQLSNFFTFKTPTISLP 128

Query: 63  SKGISSAQNRTGQIAATLSPPSGEIHVIVGPMFAGKTTTLLRRIQSESSNGRSVAIIKSN 122
           SKG  S QNR  Q+ A+LSPPSGEIHVI+GPMFAGKTTTLLRRIQSES NGRSVAIIKSN
Sbjct: 129 SKGFFSTQNRNPQMRASLSPPSGEIHVILGPMFAGKTTTLLRRIQSESCNGRSVAIIKSN 188

Query: 123 KDTRYGLDSIVTHDGMKLPCWALPNLTSFKQKFGQGAYDKLDVIGIDEAQFFDDLYDFCR 182
           KDTRYGLDSIVTHDGMKLPCWA+PNL+SFK+KFGQG+YDKLDVIGIDEAQFFDDLYDFC 
Sbjct: 189 KDTRYGLDSIVTHDGMKLPCWAIPNLSSFKKKFGQGSYDKLDVIGIDEAQFFDDLYDFCC 248

Query: 183 EAADIDGKTVIVAGLDGDYLRRNFGSVLNIIPLADSVTKLTARCEICGNRAFFTLRKTEE 242
           EAADIDGKTVIVAGLDGDYLRRNFGSVL+IIPLADSVTKLTARCEICGNRAFFTLRKT+E
Sbjct: 249 EAADIDGKTVIVAGLDGDYLRRNFGSVLDIIPLADSVTKLTARCEICGNRAFFTLRKTQE 308

Query: 243 KETELIGGADVYMPVCRQHYVSGQVVIETARTVVESHKLECRTPA 288
           KETELIGGAD+YMPVCRQHYVSGQV IETARTVVES K+  RTPA
Sbjct: 309 KETELIGGADMYMPVCRQHYVSGQVAIETARTVVESRKVGYRTPA 353

[Close]

BLAST of Lsi02G011250.1 vs. TrEMBL
Match: A0A0D2UIS2_GOSRA (Thymidine kinase OS=Gossypium raimondii GN=B456_010G249600 PE=3 SV=1)

HSP 1 Score: 371.7 bits (953), Expect = 8.0e-100
Identity = 201/287 (70.03%), Postives = 222/287 (77.35%), Query Frame = 1


Query: 3   TISKMKSLVSSSSFSTLSPYFPITASPTFFSLPSKSTQCS-PIFTQVSNYFTFKTPRILF 62
           +IS MKSL+S   FS L  +FP T    FFSLPSK    S P F           P +  
Sbjct: 20  SISSMKSLISPP-FSPLILHFPKTKPFAFFSLPSKPPNVSYPKFLHAK-------PSMPS 79

Query: 63  PSKGISSAQNRTGQIAATLSP-PSGEIHVIVGPMFAGKTTTLLRRIQSESSNGRSVAIIK 122
           P   I S   R   I    S  P GE+HVIVGPMFAGKTTTLL RIQ+ES+NGR+VA+IK
Sbjct: 80  PRSPIFSIPIRQSHIETPQSSLPCGEVHVIVGPMFAGKTTTLLHRIQAESNNGRTVAVIK 139

Query: 123 SNKDTRYGLDSIVTHDGMKLPCWALPNLTSFKQKFGQGAYDKLDVIGIDEAQFFDDLYDF 182
           SNKDTRYGLDSIVTHDGMKLPCWAL NL+SF+QK G  AYD+LDVIGIDEAQFF+DLYDF
Sbjct: 140 SNKDTRYGLDSIVTHDGMKLPCWALANLSSFRQKIGPDAYDQLDVIGIDEAQFFEDLYDF 199

Query: 183 CREAADIDGKTVIVAGLDGDYLRRNFGSVLNIIPLADSVTKLTARCEICGNRAFFTLRKT 242
           C EAAD D KTVIVAGLDGDYLRR+FGSVLNIIPLA+SVTKLTARCE+CG RAFFTLRKT
Sbjct: 200 CCEAADHDCKTVIVAGLDGDYLRRSFGSVLNIIPLANSVTKLTARCELCGKRAFFTLRKT 259

Query: 243 EEKETELIGGADVYMPVCRQHYVSGQVVIETARTVVESHKLECRTPA 288
           +E ETELIGGA+VYMPVCRQHYV GQVVI  AR+V+ES KL+C T A
Sbjct: 260 DETETELIGGAEVYMPVCRQHYVDGQVVIGAARSVLESKKLQCGTCA 298

[Close]

BLAST of Lsi02G011250.1 vs. TrEMBL
Match: A0A0B0NWB9_GOSAR (Thymidine kinase OS=Gossypium arboreum GN=F383_05181 PE=3 SV=1)

HSP 1 Score: 367.9 bits (943), Expect = 1.2e-98
Identity = 198/285 (69.47%), Postives = 223/285 (78.25%), Query Frame = 1


Query: 3   TISKMKSLVSSSSFSTLSPYFPITASPTFFSLPSKSTQCS-PIFTQVSNYFTFKTPRILF 62
           +IS MKSL+    FS LS +FP T    FFSLPSK+   S P F  V+       P +  
Sbjct: 5   SISSMKSLIFPP-FSPLSLHFPKTKPFAFFSLPSKTPNVSYPKFLHVN-------PSMPS 64

Query: 63  PSKGISSAQNRTGQIAATLSPP-SGEIHVIVGPMFAGKTTTLLRRIQSESSNGRSVAIIK 122
           P   I S   R   I    S    GE+HVIVGPMFAGKTTTLL RIQ+ES+NGR+VA+IK
Sbjct: 65  PRPPIFSIPIRQSHIETPQSSSLCGEVHVIVGPMFAGKTTTLLHRIQAESNNGRTVAVIK 124

Query: 123 SNKDTRYGLDSIVTHDGMKLPCWALPNLTSFKQKFGQGAYDKLDVIGIDEAQFFDDLYDF 182
           SNKDTRYGLDSIVTHDGMKLPCWAL +L+SF+ K G  AYD+LDVIGIDEAQFF+DLYDF
Sbjct: 125 SNKDTRYGLDSIVTHDGMKLPCWALASLSSFRLKIGPDAYDQLDVIGIDEAQFFEDLYDF 184

Query: 183 CREAADIDGKTVIVAGLDGDYLRRNFGSVLNIIPLADSVTKLTARCEICGNRAFFTLRKT 242
           C EAAD DGKTVIVAGLDGDYLRR+FGSVLNIIPLA+SVTKLTA+CE+CG RAFFTLRKT
Sbjct: 185 CCEAADHDGKTVIVAGLDGDYLRRSFGSVLNIIPLANSVTKLTAQCELCGKRAFFTLRKT 244

Query: 243 EEKETELIGGADVYMPVCRQHYVSGQVVIETARTVVESHKLECRT 286
           +E ETELIGGA+VYMPVCRQHYV GQVVI  AR+V+ES KL+C T
Sbjct: 245 DETETELIGGAEVYMPVCRQHYVDGQVVIGAARSVLESKKLQCGT 281

[Close]

BLAST of Lsi02G011250.1 vs. TrEMBL
Match: A0A067LDR5_JATCU (Thymidine kinase OS=Jatropha curcas GN=JCGZ_02929 PE=3 SV=1)

HSP 1 Score: 366.7 bits (940), Expect = 2.6e-98
Identity = 197/267 (73.78%), Postives = 217/267 (81.27%), Query Frame = 1


Query: 21  PYFPI---TASPTFFSLPSK-STQCSPIFTQVSNYFTFKTPRILFPSKGISSAQNRTGQI 80
           P+ PI   T+S  FFSL SK     SP   +  N+   K P    P   I   QNR  Q 
Sbjct: 4   PFSPICRPTSSALFFSLASKYKFLSSPSTLKNVNFLPIKPP----PPVPIFPIQNRCLQS 63

Query: 81  AATLSPPSGEIHVIVGPMFAGKTTTLLRRIQSESSNGRSVAIIKSNKDTRYGLDSIVTHD 140
            ++ S  SGEIHVIVGPMFAGKTTTLLRRI+SES +GR+VAIIKSNKDTRYGLDSIVTHD
Sbjct: 64  KSSPSSSSGEIHVIVGPMFAGKTTTLLRRIESESRDGRNVAIIKSNKDTRYGLDSIVTHD 123

Query: 141 GMKLPCWALPNLTSFKQKFGQGAYDKLDVIGIDEAQFFDDLYDFCREAADIDGKTVIVAG 200
           G+KLPC ALPNL+SF+QKFG  AY++LDVIGIDEAQFF+DLYDFCREAAD DGKTVIVAG
Sbjct: 124 GLKLPCVALPNLSSFRQKFGADAYEQLDVIGIDEAQFFEDLYDFCREAADHDGKTVIVAG 183

Query: 201 LDGDYLRRNFGSVLNIIPLADSVTKLTARCEICGNRAFFTLRKTEEKETELIGGADVYMP 260
           LDGDYLR++FGSVL+IIPLADSVTKLTARCEICG+RAFFTLRKTEE   ELIGGADVYMP
Sbjct: 184 LDGDYLRKSFGSVLDIIPLADSVTKLTARCEICGSRAFFTLRKTEETRVELIGGADVYMP 243

Query: 261 VCRQHYVSGQVVIE-TARTVVESHKLE 283
           VCRQHYVSGQVVIE  AR VVES K+E
Sbjct: 244 VCRQHYVSGQVVIEAAARIVVESQKVE 266

[Close]

BLAST of Lsi02G011250.1 vs. TrEMBL
Match: A0A0B2REH6_GLYSO (Thymidine kinase OS=Glycine soja GN=glysoja_009947 PE=3 SV=1)

HSP 1 Score: 365.5 bits (937), Expect = 5.8e-98
Identity = 197/283 (69.61%), Postives = 226/283 (79.86%), Query Frame = 1


Query: 7   MKSLVSSSSFSTLSPYFPITASP-TFFSLPSKST-----QCSPIFTQVSNYFTFKTPRIL 66
           MKSL++   FS LSP     ASP   FSLPS+ST     + +P   ++ N      P   
Sbjct: 1   MKSLLNPK-FSALSP----KASPFALFSLPSQSTTRFSFRSNP--RRIPNRLLRLNPPPF 60

Query: 67  FPSKGISSAQNRTGQIAATLSPPSGEIHVIVGPMFAGKTTTLLRRIQSESSNGRSVAIIK 126
             +  I + QNR+ Q   +  PPSGEIHVIVGPMFAGKTT+LLRRIQSE++NGR+VAIIK
Sbjct: 61  SSNNFICAIQNRSLQTEPS-PPPSGEIHVIVGPMFAGKTTSLLRRIQSETANGRNVAIIK 120

Query: 127 SNKDTRYGLDSIVTHDGMKLPCWALPNLTSFKQKFGQGAYDKLDVIGIDEAQFFDDLYDF 186
           S+KDTRYGLDSIVTHDG KLPCWAL NL+SFKQKFG  AY+KLDVIGIDEAQFFDDLY+F
Sbjct: 121 SSKDTRYGLDSIVTHDGAKLPCWALANLSSFKQKFGMDAYEKLDVIGIDEAQFFDDLYEF 180

Query: 187 CREAADIDGKTVIVAGLDGDYLRRNFGSVLNIIPLADSVTKLTARCEICGNRAFFTLRKT 246
           CR+AAD DGKTVIVAGLDG+YLRR+FGSVL+IIPLADSVTKLTARCEICG RA FTLRKT
Sbjct: 181 CRQAADHDGKTVIVAGLDGNYLRRSFGSVLDIIPLADSVTKLTARCEICGKRACFTLRKT 240

Query: 247 EEKETELIGGADVYMPVCRQHYVSGQVVIETARTVVESHKLEC 284
           ++K+ ELIGG DVYMPVCRQHYVSGQV +E  R V+ES K+EC
Sbjct: 241 QDKQIELIGGVDVYMPVCRQHYVSGQVAMEATRHVLESKKVEC 275

[Close]

BLAST of Lsi02G011250.1 vs. TAIR10
Match: AT5G23070.1 (AT5G23070.1 Thymidine kinase)

HSP 1 Score: 308.9 bits (790), Expect = 3.2e-84
Identity = 163/274 (59.49%), Postives = 208/274 (75.91%), Query Frame = 1


Query: 7   MKSLVSSSSFSTLSPYFPITASPTFFSLPSK-STQCSPIFTQVSNYFTFKTPRILFPSKG 66
           M++L+S S    L+P+      P+ FS   + S   + I    S   T  T ++   +  
Sbjct: 6   MRTLISPS----LAPFSLHLHKPSLFSTALRFSFSINNITPTNSPPSTISTRKLQTKATR 65

Query: 67  ISSAQNRTGQIAATLSPPSGEIHVIVGPMFAGKTTTLLRRIQSESSNGRSVAIIKSNKDT 126
           ++S+ +   Q  ++ SP  GEIHV+VGPMF+GKTTTLLRRI +E   G+ +AIIKSNKDT
Sbjct: 66  VTSSSS--SQPLSSSSP--GEIHVVVGPMFSGKTTTLLRRILAERETGKRIAIIKSNKDT 125

Query: 127 RYGLDSIVTHDGMKLPCWALPNLTSFKQKFGQGAYD-KLDVIGIDEAQFFDDLYDFCREA 186
           RY  +SIVTHDG K PCW+LP+L+SFK++FG   Y+ +LDVIGIDEAQFF DLY+FCREA
Sbjct: 126 RYCTESIVTHDGEKYPCWSLPDLSSFKERFGFDDYENRLDVIGIDEAQFFGDLYEFCREA 185

Query: 187 ADIDGKTVIVAGLDGDYLRRNFGSVLNIIPLADSVTKLTARCEICGNRAFFTLRKTEEKE 246
           AD +GKTVIVAGLDGD++RR FGSVL++IP+AD+VTKLT+RCE+CG RA FT+RKTEEKE
Sbjct: 186 ADKEGKTVIVAGLDGDFMRRRFGSVLDLIPIADTVTKLTSRCEVCGKRALFTMRKTEEKE 245

Query: 247 TELIGGADVYMPVCRQHYVSGQVVIETARTVVES 279
           TELIGGA+VYMPVCR HYV GQ V+ETAR V++S
Sbjct: 246 TELIGGAEVYMPVCRSHYVCGQNVLETARAVLDS 271

[Close]

BLAST of Lsi02G011250.1 vs. TAIR10
Match: AT3G07800.1 (AT3G07800.1 Thymidine kinase)

HSP 1 Score: 300.1 bits (767), Expect = 1.5e-81
Identity = 138/194 (71.13%), Postives = 171/194 (88.14%), Query Frame = 1


Query: 84  SGEIHVIVGPMFAGKTTTLLRRIQSESSNGRSVAIIKSNKDTRYGLDSIVTHDGMKLPCW 143
           SG +HVI+GPMF+GK+T+LLRRI+SE S+GRSVA++KS+KDTRY  DS+VTHDG+  PCW
Sbjct: 30  SGAVHVIMGPMFSGKSTSLLRRIKSEISDGRSVAMLKSSKDTRYAKDSVVTHDGIGFPCW 89

Query: 144 ALPNLTSFKQKFGQGAYDKLDVIGIDEAQFFDDLYDFCREAADIDGKTVIVAGLDGDYLR 203
           ALP+L SF +KFG  AY+KLDVIGIDEAQFF DLY+FC + AD DGK VIVAGLDGDYLR
Sbjct: 90  ALPDLMSFPEKFGLDAYNKLDVIGIDEAQFFGDLYEFCCKVADDDGKIVIVAGLDGDYLR 149

Query: 204 RNFGSVLNIIPLADSVTKLTARCEICGNRAFFTLRKTEEKETELIGGADVYMPVCRQHYV 263
           R+FG+VL+IIP+ADSVTKLTARCE+CG++AFFTLRK  +  TELIGGADVYMPVCR+HY+
Sbjct: 150 RSFGAVLDIIPIADSVTKLTARCEVCGHKAFFTLRKNCDTRTELIGGADVYMPVCRKHYI 209

Query: 264 SGQVVIETARTVVE 278
           +  +VI+ ++ V+E
Sbjct: 210 TNHIVIKASKKVLE 223

[Close]

BLAST of Lsi02G011250.1 vs. NCBI nr
Match: gi|659127259|ref|XP_008463610.1| (PREDICTED: thymidine kinase-like [Cucumis melo])

HSP 1 Score: 504.6 bits (1298), Expect = 1.1e-139
Identity = 249/281 (88.61%), Postives = 263/281 (93.59%), Query Frame = 1


Query: 7   MKSLVSSSSFSTLSPYFPITASPTFFSLPSKSTQCSPIFTQVSNYFTFKTPRILFPSKGI 66
           MKSLVSSSSFS LSPYFPI+ASP+FFSLPSKS QC P+FTQVSN+FTFKTP    PSKG 
Sbjct: 1   MKSLVSSSSFSILSPYFPISASPSFFSLPSKSAQCGPMFTQVSNFFTFKTPTTSLPSKGF 60

Query: 67  SSAQNRTGQIAATLSPPSGEIHVIVGPMFAGKTTTLLRRIQSESSNGRSVAIIKSNKDTR 126
            S QNR  Q  A+LSPPSGEIHVI+GPMFAGKTTTLLRRIQSES NGR+VAIIKSNKDTR
Sbjct: 61  FSTQNRNPQTRASLSPPSGEIHVILGPMFAGKTTTLLRRIQSESCNGRNVAIIKSNKDTR 120

Query: 127 YGLDSIVTHDGMKLPCWALPNLTSFKQKFGQGAYDKLDVIGIDEAQFFDDLYDFCREAAD 186
           YGLDSIVTHDGMKLPCWA+PNL+SFK+KFGQG+YDKLDVIGIDEAQFFDDLYDFC EAAD
Sbjct: 121 YGLDSIVTHDGMKLPCWAIPNLSSFKKKFGQGSYDKLDVIGIDEAQFFDDLYDFCCEAAD 180

Query: 187 IDGKTVIVAGLDGDYLRRNFGSVLNIIPLADSVTKLTARCEICGNRAFFTLRKTEEKETE 246
           IDGKTVIVAGLDGDYLRRNFGSVL+IIPLADSVTKLTARCEICGNRAFFTLRKT+EKETE
Sbjct: 181 IDGKTVIVAGLDGDYLRRNFGSVLDIIPLADSVTKLTARCEICGNRAFFTLRKTQEKETE 240

Query: 247 LIGGADVYMPVCRQHYVSGQVVIETARTVVESHKLECRTPA 288
           LIGGADVYMPVCRQHYVSGQVVIE ARTVVES K+ECRTPA
Sbjct: 241 LIGGADVYMPVCRQHYVSGQVVIEAARTVVESRKVECRTPA 281

[Close]

BLAST of Lsi02G011250.1 vs. NCBI nr
Match: gi|700209940|gb|KGN65036.1| (Thymidine kinase [Cucumis sativus])

HSP 1 Score: 500.4 bits (1287), Expect = 2.2e-138
Identity = 248/285 (87.02%), Postives = 264/285 (92.63%), Query Frame = 1


Query: 3   TISKMKSLVSSSSFSTLSPYFPITASPTFFSLPSKSTQCSPIFTQVSNYFTFKTPRILFP 62
           TIS MKS V SSSFS LSPYFPI+ASP+FFSLPSKS QC P+FTQ+SN+FTFKTP I  P
Sbjct: 69  TISNMKSFVFSSSFSILSPYFPISASPSFFSLPSKSAQCGPMFTQLSNFFTFKTPTISLP 128

Query: 63  SKGISSAQNRTGQIAATLSPPSGEIHVIVGPMFAGKTTTLLRRIQSESSNGRSVAIIKSN 122
           SKG  S QNR  Q+ A+LSPPSGEIHVI+GPMFAGKTTTLLRRIQSES NGRSVAIIKSN
Sbjct: 129 SKGFFSTQNRNPQMRASLSPPSGEIHVILGPMFAGKTTTLLRRIQSESCNGRSVAIIKSN 188

Query: 123 KDTRYGLDSIVTHDGMKLPCWALPNLTSFKQKFGQGAYDKLDVIGIDEAQFFDDLYDFCR 182
           KDTRYGLDSIVTHDGMKLPCWA+PNL+SFK+KFGQG+YDKLDVIGIDEAQFFDDLYDFC 
Sbjct: 189 KDTRYGLDSIVTHDGMKLPCWAIPNLSSFKKKFGQGSYDKLDVIGIDEAQFFDDLYDFCC 248

Query: 183 EAADIDGKTVIVAGLDGDYLRRNFGSVLNIIPLADSVTKLTARCEICGNRAFFTLRKTEE 242
           EAADIDGKTVIVAGLDGDYLRRNFGSVL+IIPLADSVTKLTARCEICGNRAFFTLRKT+E
Sbjct: 249 EAADIDGKTVIVAGLDGDYLRRNFGSVLDIIPLADSVTKLTARCEICGNRAFFTLRKTQE 308

Query: 243 KETELIGGADVYMPVCRQHYVSGQVVIETARTVVESHKLECRTPA 288
           KETELIGGAD+YMPVCRQHYVSGQV IETARTVVES K+  RTPA
Sbjct: 309 KETELIGGADMYMPVCRQHYVSGQVAIETARTVVESRKVGYRTPA 353

[Close]

BLAST of Lsi02G011250.1 vs. NCBI nr
Match: gi|778659746|ref|XP_011654956.1| (PREDICTED: thymidine kinase [Cucumis sativus])

HSP 1 Score: 495.7 bits (1275), Expect = 5.3e-137
Identity = 245/281 (87.19%), Postives = 261/281 (92.88%), Query Frame = 1


Query: 7   MKSLVSSSSFSTLSPYFPITASPTFFSLPSKSTQCSPIFTQVSNYFTFKTPRILFPSKGI 66
           MKS V SSSFS LSPYFPI+ASP+FFSLPSKS QC P+FTQ+SN+FTFKTP I  PSKG 
Sbjct: 1   MKSFVFSSSFSILSPYFPISASPSFFSLPSKSAQCGPMFTQLSNFFTFKTPTISLPSKGF 60

Query: 67  SSAQNRTGQIAATLSPPSGEIHVIVGPMFAGKTTTLLRRIQSESSNGRSVAIIKSNKDTR 126
            S QNR  Q+ A+LSPPSGEIHVI+GPMFAGKTTTLLRRIQSES NGRSVAIIKSNKDTR
Sbjct: 61  FSTQNRNPQMRASLSPPSGEIHVILGPMFAGKTTTLLRRIQSESCNGRSVAIIKSNKDTR 120

Query: 127 YGLDSIVTHDGMKLPCWALPNLTSFKQKFGQGAYDKLDVIGIDEAQFFDDLYDFCREAAD 186
           YGLDSIVTHDGMKLPCWA+PNL+SFK+KFGQG+YDKLDVIGIDEAQFFDDLYDFC EAAD
Sbjct: 121 YGLDSIVTHDGMKLPCWAIPNLSSFKKKFGQGSYDKLDVIGIDEAQFFDDLYDFCCEAAD 180

Query: 187 IDGKTVIVAGLDGDYLRRNFGSVLNIIPLADSVTKLTARCEICGNRAFFTLRKTEEKETE 246
           IDGKTVIVAGLDGDYLRRNFGSVL+IIPLADSVTKLTARCEICGNRAFFTLRKT+EKETE
Sbjct: 181 IDGKTVIVAGLDGDYLRRNFGSVLDIIPLADSVTKLTARCEICGNRAFFTLRKTQEKETE 240

Query: 247 LIGGADVYMPVCRQHYVSGQVVIETARTVVESHKLECRTPA 288
           LIGGAD+YMPVCRQHYVSGQV IETARTVVES K+  RTPA
Sbjct: 241 LIGGADMYMPVCRQHYVSGQVAIETARTVVESRKVGYRTPA 281

[Close]

BLAST of Lsi02G011250.1 vs. NCBI nr
Match: gi|1021508407|ref|XP_016197554.1| (PREDICTED: thymidine kinase a-like [Arachis ipaensis])

HSP 1 Score: 375.2 bits (962), Expect = 1.0e-100
Identity = 199/279 (71.33%), Postives = 226/279 (81.00%), Query Frame = 1


Query: 7   MKSLVSSSSFSTLSPYFPITASPTFFSLPSKSTQCSPIFTQVSNYFTFKTPRIL-FPSKG 66
           MKS+++   FS LSP+ P T+  + FS P  S     IF + S    F + +   F S  
Sbjct: 1   MKSILNPK-FSALSPHLPKTSPFSLFSRPHDS-----IFRKNSQQLPFPSLKATPFSSNS 60

Query: 67  -ISSAQNRTGQIAATLSPPSGEIHVIVGPMFAGKTTTLLRRIQSESSNGRSVAIIKSNKD 126
            +S AQNRT Q   T  PPSGEIHVIVGPMFAGKTTTLLRRIQSES+N ++VAIIKS+KD
Sbjct: 61  FLSRAQNRTLQTEPT-RPPSGEIHVIVGPMFAGKTTTLLRRIQSESANSKNVAIIKSSKD 120

Query: 127 TRYGLDSIVTHDGMKLPCWALPNLTSFKQKFGQGAYDKLDVIGIDEAQFFDDLYDFCREA 186
           TRYGLDSIVTHDG KLPCWAL NL+SFKQKFG  AY+KLDVIGIDEAQFF+DLYDFCREA
Sbjct: 121 TRYGLDSIVTHDGAKLPCWALTNLSSFKQKFGIEAYEKLDVIGIDEAQFFEDLYDFCREA 180

Query: 187 ADIDGKTVIVAGLDGDYLRRNFGSVLNIIPLADSVTKLTARCEICGNRAFFTLRKTEEKE 246
           AD DGKTVIVAGLDG+YLRR+FGSVL+IIPLAD+VTKLTA CEICG RAFFTLRKT+EK+
Sbjct: 181 ADHDGKTVIVAGLDGNYLRRSFGSVLDIIPLADTVTKLTALCEICGKRAFFTLRKTQEKQ 240

Query: 247 TELIGGADVYMPVCRQHYVSGQVVIETARTVVESHKLEC 284
            ELIGG DVYMPVCR HYV+GQV +E AR V+ESHK+EC
Sbjct: 241 VELIGGGDVYMPVCRHHYVNGQVAVEAARFVLESHKVEC 272

[Close]

BLAST of Lsi02G011250.1 vs. NCBI nr
Match: gi|743893916|ref|XP_011040200.1| (PREDICTED: thymidine kinase-like [Populus euphratica])

HSP 1 Score: 374.4 bits (960), Expect = 1.8e-100
Identity = 200/283 (70.67%), Postives = 225/283 (79.51%), Query Frame = 1


Query: 2   LTISKMKSLVSSS-SFSTLSPYFPITASPTFFSLPSKSTQCSPIFTQVSNYFTFKTPRIL 61
           +TIS+MK+L+  + SFS +S    I  +  FFSL SKS   S +   VS YF  K   I 
Sbjct: 4   ITISRMKALIIPTFSFSPMSKR-AIPLASNFFSLASKSNLFSNLQNPVS-YFPLKPSMIS 63

Query: 62  FPSKGISSAQNRTGQIAATLSPPSGEIHVIVGPMFAGKTTTLLRRIQSESSNGRSVAIIK 121
            PS      Q R     ++LS  SGEIHVIVGPMFAGKTTTLL R+Q+E ++GR+VAIIK
Sbjct: 64  LPSN--FPIQTRCVHSKSSLSSSSGEIHVIVGPMFAGKTTTLLHRVQAEINDGRNVAIIK 123

Query: 122 SNKDTRYGLDSIVTHDGMKLPCWALPNLTSFKQKFGQGAYDKLDVIGIDEAQFFDDLYDF 181
           SNKD RYGLDS+VTHDG+KLPC ALPNL+SFKQ FGQ AYD+LDVIGIDEAQFF DLYDF
Sbjct: 124 SNKDNRYGLDSVVTHDGVKLPCCALPNLSSFKQSFGQDAYDQLDVIGIDEAQFFGDLYDF 183

Query: 182 CREAADIDGKTVIVAGLDGDYLRRNFGSVLNIIPLADSVTKLTARCEICGNRAFFTLRKT 241
           CRE AD DGKTVIVAGLDGDYLRRNFGSVL+IIPLADSVTKL+ARCEICG RAFFTLRKT
Sbjct: 184 CREVADHDGKTVIVAGLDGDYLRRNFGSVLDIIPLADSVTKLSARCEICGKRAFFTLRKT 243

Query: 242 EEKETELIGGADVYMPVCRQHYVSGQVVIETARTVVESHKLEC 284
           EE +TELIGGADVYMPVCRQHYVSGQV +E AR V+ES K +C
Sbjct: 244 EETQTELIGGADVYMPVCRQHYVSGQVAVEAARMVLESQKAQC 282

The following BLAST results are available for this feature:

BLAST of Lsi02G011250.1 vs. Swiss-Prot


Match Name	E-value	Identity	Description
KITH_ORYSJ	1.6e-85	69.60	Thymidine kinase OS=Oryza sativa subsp. japonica GN=TK PE=2 SV=2	[more]
KITHB_ARATH	5.7e-83	59.49	Thymidine kinase b OS=Arabidopsis thaliana GN=TK1B PE=1 SV=1	[more]
KITHA_ARATH	2.7e-80	71.13	Thymidine kinase a OS=Arabidopsis thaliana GN=TK1A PE=1 SV=1	[more]
KITH_SWPVK	1.9e-38	45.00	Thymidine kinase OS=Swinepox virus (strain Kasza) GN=TK PE=3 SV=1	[more]
KITH_CHICK	3.4e-35	45.36	Thymidine kinase, cytosolic OS=Gallus gallus GN=TK1 PE=1 SV=1	[more]

BLAST of Lsi02G011250.1 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LYL4_CUCSA	1.5e-138	87.02	Thymidine kinase OS=Cucumis sativus GN=Csa_1G181480 PE=3 SV=1	[more]
A0A0D2UIS2_GOSRA	8.0e-100	70.03	Thymidine kinase OS=Gossypium raimondii GN=B456_010G249600 PE=3 SV=1	[more]
A0A0B0NWB9_GOSAR	1.2e-98	69.47	Thymidine kinase OS=Gossypium arboreum GN=F383_05181 PE=3 SV=1	[more]
A0A067LDR5_JATCU	2.6e-98	73.78	Thymidine kinase OS=Jatropha curcas GN=JCGZ_02929 PE=3 SV=1	[more]
A0A0B2REH6_GLYSO	5.8e-98	69.61	Thymidine kinase OS=Glycine soja GN=glysoja_009947 PE=3 SV=1	[more]

BLAST of Lsi02G011250.1 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G23070.1	3.2e-84	59.49	Thymidine kinase	[more]
AT3G07800.1	1.5e-81	71.13	Thymidine kinase	[more]

BLAST of Lsi02G011250.1 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659127259\|ref\|XP_008463610.1\|	1.1e-139	88.61	PREDICTED: thymidine kinase-like [Cucumis melo]	[more]
gi\|700209940\|gb\|KGN65036.1\|	2.2e-138	87.02	Thymidine kinase [Cucumis sativus]	[more]
gi\|778659746\|ref\|XP_011654956.1\|	5.3e-137	87.19	PREDICTED: thymidine kinase [Cucumis sativus]	[more]
gi\|1021508407\|ref\|XP_016197554.1\|	1.0e-100	71.33	PREDICTED: thymidine kinase a-like [Arachis ipaensis]	[more]
gi\|743893916\|ref\|XP_011040200.1\|	1.8e-100	70.67	PREDICTED: thymidine kinase-like [Populus euphratica]	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **Molecular Function**
Term	Definition
GO:0005524	ATP binding
GO:0004797	thymidine kinase activity

Vocabulary: **INTERPRO**
Term	Definition
IPR027417	P-loop_NTPase
IPR020633	Thymidine_kinase_CS
IPR001267	Thymidine_kinase

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009157	deoxyribonucleoside monophosphate biosynthetic process
biological_process	GO:0071897	DNA biosynthetic process
biological_process	GO:0016310	phosphorylation
biological_process	GO:0006206	pyrimidine nucleobase metabolic process
biological_process	GO:0046104	thymidine metabolic process
biological_process	GO:0006230	TMP biosynthetic process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004797	thymidine kinase activity

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Lsi02G011250	Lsi02G011250	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Lsi02G011250.1	Lsi02G011250.1-protein	polypeptide

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lsi02G011250.1.five_prime_UTR.1	Lsi02G011250.1.five_prime_UTR.1	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lsi02G011250.1.CDS.1	Lsi02G011250.1.CDS.1	CDS
Lsi02G011250.1.CDS.2	Lsi02G011250.1.CDS.2	CDS
Lsi02G011250.1.CDS.3	Lsi02G011250.1.CDS.3	CDS
Lsi02G011250.1.CDS.4	Lsi02G011250.1.CDS.4	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lsi02G011250.1.three_prime_UTR.1	Lsi02G011250.1.three_prime_UTR.1	three_prime_UTR

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001267	Thymidine kinase	PANTHER	PTHR11441	THYMIDINE KINASE	coord: 71..283 score: 8.3E
IPR001267	Thymidine kinase	PFAM	PF00265	TK	coord: 85..262 score: 3.3
IPR020633	Thymidine kinase, conserved site	PROSITE	PS00603	TK_CELLULAR_TYPE	coord: 249..262 scor
IPR027417	P-loop containing nucleoside triphosphate hydrolase	unknown	SSF52540	P-loop containing nucleoside triphosphate hydrolases	coord: 85..223 score: 7.8
None	No IPR available	GENE3D	G3DSA:3.30.60.20		coord: 223..264 score: 9.0
None	No IPR available	PANTHER	PTHR11441:SF2	THYMIDINE KINASE	coord: 71..283 score: 8.3E
None	No IPR available	unknown	SSF57716	Glucocorticoid receptor-like (DNA-binding domain)	coord: 223..265 score: 8.55