Lsi01G016270.1 (mRNA) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi01G016270.1
Type	mRNA
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Ubiquitin-conjugating enzyme E2
Location	chr01 : 14640705 .. 14644056 (-)
Sequence length	1631

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCCTTTGTTTGCTTTGTCATAAGCTTGATGGGTTTTTTGAATCTTCTCGTTTTTGTGTGTAGATCATGGCTTCGAAGCGTATCTTGAAGGAACTTAAGGATTTACAGAAGGATCCACCCACATCTTGCAGTGCTGGTATGGTTCTAAGCCTTGATCTCTTTATTTGGTGACCTATTTTGACCATTTTCTTCGGATTTATTGCGTTTGTTTTGTTGATGGGAATGAATGAGTGAATAAAATGAATTGGTATCTGTTGATTGGTTTTTTTTGTGTTGTTGACCTTTTGATCTGTCTTGATGTGAAGATGTTTATTTGTTTTCTAGTTTTGAGCTTGGGATGTTTTTTTTAGAGATTTTTGTATGGATGACCCAATCGACGCCCTAATCCTAGAAAAACAAATGAAAATTAACCTTCAGCTGATTTCACAAAGCACTATCATGTTTGGAGCGTCTCTCCCATCGGAAAATAGTTGTAGCCCTAACGAGATATTCATTTCATTGCAATTTTTTAAAAATCAACCATTTTTTTTAATTGCAATGATTGAAAATGAAAATCATGAAATGGTCGAGTGAGACGTCCCTAACGTGATAATGGGTGGTAAATTGCTCTATGACGTAAGCAGAATGTTAGTTTTCATTGACTTTTTAGAATTGGTTTATTCAACATTAACTAACGCCGTTGGAATTGGGTCGTCCGTACAAAAATCTTTGTTTTTATTTTATTTTATTTATTTCGTTTGCTTTCGAGGATAGGCCCTGTAGCTGAAGATATGTTCCATTGGCAAGCGACCATTATGGGACCATCTGATAGTCCTTATTCAGGAGGTGTTTTTCTTGTCAATATTCATTTTCCTCCAGATTATCCTTTCAAGCCCCCCAAGGTGAATGATGTTTGTCGTTTGTTTGTTGCTCTTAATGATTGCGCAAGATGATTCTATGTTTGTTGTTTCCTTTTCGAATATAATAGGATTGCGAGGTTTAATAGTTATGGATTGTTGTTCAGGTTGCTTTTAGGACCAAAGTGTTTCACCCAAATATCAATAGCAATGGAAGCATTTGTCTTGATATCCTTAAAGAACAGTGGAGCCCTGCGCTTACGATTTCTAAGGTTCGTTTGGCATTTTGTTTCTGAATTCACTCATAGTTCATCATTTTGTTTCAAATTCTCTGGATTGTCATTGTGCTGTTTGCATAAATATAGCTATTTTTGTCATGTTTGAGATCTATATGCTTCTGCCATAGTAAAGTTCTGTGTAGCATGGATGCTTACTCGAAGTTCCCTATTTGATGGAGAATCTTACAACACTTCAGTAGTACTGAAAATTTCACGAACTCGGTTGTGTGGTCTATAATGGACAGCATGTTCAATTTCCATTCTTCGTTTTCTTGGCTGGCTGGTTTGATCACATAAACAGTCTGGATATTTCTTTGAGCCATGGTTTCTTTATGCAATTAGGAGTTCTTTTTTCTGTGAATAATTTGGGTCTCGCTCGACTCCTCGCATTATATTCCCGGGATGGGAGCCATTCCACGGGTAGCTTCGGGGCAGTTGAGAACAGTGGTTGATTTGTGTTTCTACCCCAAAAAGTGTTTGAAGAAGTAGTTGTAGTGTGGCCATTAGTTTGTTTGTTCTACCTTTTCCCTCTCCTTGTCTATATTTAGGAACTACTGCTGTTAAAGTGTAGAAGCCTTCATTTTACCCACATGGCTATTAAATAGCTTAGCTTGATATCTTCACTATGACCTTTTGATCATGGAATAAAATCCCATTTGAGCAGGAAACTCACATTCTCATCAACTTTTTGTTGATTTATGTTCTAGAAATGTTAAAAGTTAAGGTTGACTGACTTTGCGAGAGACCGTAATCATTACCCATCAGTCATGGCCCTCCTGACTGAAAACAAGAGAGAGACTAGAAAATTTTGACTGAGTTAATTCTAAACATAAATGGTGTTCATGAAACAGGTACTGCTCTCCATATGCTCATTGCTGACCGATCCAAACCCAGACGACCCTCTTGTACCAGAGATCGCGCATATGTACAAGACCGACCGAACCAAGTATGAAGCGAGTGCACGGAGCTGGACTCAGAAGTATGCTATGGGTTGAGACGTTTTTGTAGCTTGATGTTGATGATGGATGATGCTAGTGGTGGTATCTTTGGCATGGCATCCTTATGTATTACCTCGTTTCTTCCAGTTATTTGGGCTGTGTAGGTATTTTAAAGCAGAAAAAAAAAGAAACAAAATATGAAAAAAAAAAAAAAAAGAAAAAGAAGAAAGTCTTGTGATGTTTTTTTCCCCTTTGCCACTGATTTATGAATGAAAGATGCAAGCAGGTTGTGTAAGGTTGTCAAATTAACTGGATTCAACATTCCTCTTTGTGGGGATTTGATGCAAGCAGTTATTTATCTCATAGAGAGTAGCATTGTTCAATTTATCTTTTCTTTTTTTTTTTTTATGGGATTTTTGGTTACCAATCCCTTAAAAAGAAAAGTTTCGGGATGGGGACGGAGATGGGGATTTTCTCTGAAAATTTGTTAAGAATTGGTTTTTTAGGAACATTTTTTATATAATTTTTTTTAATTTATCCATTTATAGATGTTCATTATTAAAGGGAGAATAATGCTATATATGAAAATTTTCCTTGGAATGATATACACATTTTTATTGAAATATGTACTTTCAATATAAACGGTTGTGTAATGTATAATAGGAAAATGGGATGGGATTTAGAATTTGGTTTAGAATTGGCTGTACTTTTTTTTTTGTTCTCATGGCTATTCTCTCTCAAATAGAATGATTGTATATATATGTGTGTGTCAGTGTTTAGGCCGGTTCACGCGCAACACCTCGATTAATCTTACGAATAACCGGTTTAGTCTTTTACAACATTTGGGTGTCATTAACCATTGAAATATGTTATCAAGTTCTAGACCTTCAAAAAATAGGATTGACTTGACATGACGATTGAGTTACTGGATCGATGTAACAAATTAGGGCATGTTGCATATGGAACATTAGATGGAAATTCAAAGTTCTATACATGATGGTCAGGGACTACATCATTTACTATATGGGTTCTTCTACATGATATGCGTTGTCATTTATGTCTTCACCATTAACATTTGCGACTACTTCCTCTCCATCATCACCTGTTTCCCTCTAGGACCAATCGTTAAAATCTTGAGTTTGAGATACATGGTCCACATCATCCCTCAAATTATTCGTCGTCACCATTAACATTTGCGACTACTTCCTCTCCATCATCACCTGTTTCCCTCTAGGACCAATCGTTAAAATCTTGAGTTTGAGATACATGGTCCACATCATCCCTCAAATTATTCGTCG

mRNA sequence

ATGCCCTTTATCATGGCTTCGAAGCGTATCTTGAAGGAACTTAAGGATTTACAGAAGGATCCACCCACATCTTGCAGTGCTGGAGGTGTTTTTCTTGTCAATATTCATTTTCCTCCAGATTATCCTTTCAAGCCCCCCAAGGTTGCTTTTAGGACCAAAGTGTTTCACCCAAATATCAATAGCAATGGAAGCATTTGTCTTGATATCCTTAAAGAACAGTGGAGCCCTGCGCTTACGATTTCTAAGGTACTGCTCTCCATATGCTCATTGCTGACCGATCCAAACCCAGACGACCCTCTTGTACCAGAGATCGCGCATATGTACAAGACCGACCGAACCAAGTATGAAGCGAGTGCACGGAGCTGGACTCAGAAGTATGCTATGGGTTGAGACGTTTTTGTAGCTTGATGTTGATGATGGATGATGCTAGTGGTGGTATCTTTGGCATGGCATCCTTATGTATTACCTCGTTTCTTCCAGTTATTTGGGCTGTGTAGGTATTTTAAAGCAGAAAAAAAAAGAAACAAAATATGAAAAAAAAAAAAAAAAGAAAAAGAAGAAAGTCTTGTGATGTTTTTTTCCCCTTTGCCACTGATTTATGAATGAAAGATGCAAGCAGGTTGTGTAAGGTTGTCAAATTAACTGGATTCAACATTCCTCTTTGTGGGGATTTGATGCAAGCAGTTATTTATCTCATAGAGAGTAGCATTGTTCAATTTATCTTTTCTTTTTTTTTTTTTATGGGATTTTTGGTTACCAATCCCTTAAAAAGAAAAGTTTCGGGATGGGGACGGAGATGGGGATTTTCTCTGAAAATTTGTTAAGAATTGGTTTTTTAGGAACATTTTTTATATAATTTTTTTTAATTTATCCATTTATAGATGTTCATTATTAAAGGGAGAATAATGCTATATATGAAAATTTTCCTTGGAATGATATACACATTTTTATTGAAATATGTACTTTCAATATAAACGGTTGTGTAATGTATAATAGGAAAATGGGATGGGATTTAGAATTTGGTTTAGAATTGGCTGTACTTTTTTTTTTGTTCTCATGGCTATTCTCTCTCAAATAGAATGATTGTATATATATGTGTGTGTCAGTGTTTAGGCCGGTTCACGCGCAACACCTCGATTAATCTTACGAATAACCGGTTTAGTCTTTTACAACATTTGGGTGTCATTAACCATTGAAATATGTTATCAAGTTCTAGACCTTCAAAAAATAGGATTGACTTGACATGACGATTGAGTTACTGGATCGATGTAACAAATTAGGGCATGTTGCATATGGAACATTAGATGGAAATTCAAAGTTCTATACATGATGGTCAGGGACTACATCATTTACTATATGGGTTCTTCTACATGATATGCGTTGTCATTTATGTCTTCACCATTAACATTTGCGACTACTTCCTCTCCATCATCACCTGTTTCCCTCTAGGACCAATCGTTAAAATCTTGAGTTTGAGATACATGGTCCACATCATCCCTCAAATTATTCGTCGTCACCATTAACATTTGCGACTACTTCCTCTCCATCATCACCTGTTTCCCTCTAGGACCAATCGTTAAAATCTTGAGTTTGAGATACATGGTCCACATCATCCCTCAAATTATTCGTCG

Coding sequence (CDS)

ATGCCCTTTATCATGGCTTCGAAGCGTATCTTGAAGGAACTTAAGGATTTACAGAAGGATCCACCCACATCTTGCAGTGCTGGAGGTGTTTTTCTTGTCAATATTCATTTTCCTCCAGATTATCCTTTCAAGCCCCCCAAGGTTGCTTTTAGGACCAAAGTGTTTCACCCAAATATCAATAGCAATGGAAGCATTTGTCTTGATATCCTTAAAGAACAGTGGAGCCCTGCGCTTACGATTTCTAAGGTACTGCTCTCCATATGCTCATTGCTGACCGATCCAAACCCAGACGACCCTCTTGTACCAGAGATCGCGCATATGTACAAGACCGACCGAACCAAGTATGAAGCGAGTGCACGGAGCTGGACTCAGAAGTATGCTATGGGTTGA

Protein sequence

MPFIMASKRILKELKDLQKDPPTSCSAGGVFLVNIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKTDRTKYEASARSWTQKYAMG

Homology

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BLAST of Lsi01G016270.1 vs. Swiss-Prot
Match: UBC8_ARATH (Ubiquitin-conjugating enzyme E2 8 OS=Arabidopsis thaliana GN=UBC8 PE=1 SV=1)

HSP 1 Score: 244.2 bits (622), Expect = 7.8e-64
Identity = 121/148 (81.76%), Postives = 123/148 (83.11%), Query Frame = 1


Query: 5   MASKRILKELKDLQKDPPTSCSAG-----------------------GVFLVNIHFPPDY 64
           MASKRILKELKDLQKDPPTSCSAG                       GVFLV IHFPPDY
Sbjct: 1   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPAESPYSGGVFLVTIHFPPDY 60

Query: 65  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 124
           PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV
Sbjct: 61  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120

Query: 125 PEIAHMYKTDRTKYEASARSWTQKYAMG 130
           PEIAHMYKTDR KYEA+AR+WTQKYAMG
Sbjct: 121 PEIAHMYKTDRAKYEATARNWTQKYAMG 148

[Close]

BLAST of Lsi01G016270.1 vs. Swiss-Prot
Match: UBC4_SOLLC (Ubiquitin-conjugating enzyme E2-17 kDa OS=Solanum lycopersicum PE=2 SV=1)

HSP 1 Score: 244.2 bits (622), Expect = 7.8e-64
Identity = 121/148 (81.76%), Postives = 123/148 (83.11%), Query Frame = 1


Query: 5   MASKRILKELKDLQKDPPTSCSAG-----------------------GVFLVNIHFPPDY 64
           MASKRILKELKDLQKDPPTSCSAG                       GVFLV+IHFPPDY
Sbjct: 1   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPTDSPYAGGVFLVSIHFPPDY 60

Query: 65  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 124
           PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV
Sbjct: 61  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120

Query: 125 PEIAHMYKTDRTKYEASARSWTQKYAMG 130
           PEIAHMYKTDR KYE +ARSWTQKYAMG
Sbjct: 121 PEIAHMYKTDRAKYETTARSWTQKYAMG 148

[Close]

BLAST of Lsi01G016270.1 vs. Swiss-Prot
Match: UBC28_ARATH (Ubiquitin-conjugating enzyme E2 28 OS=Arabidopsis thaliana GN=UBC28 PE=2 SV=1)

HSP 1 Score: 243.8 bits (621), Expect = 1.0e-63
Identity = 120/148 (81.08%), Postives = 123/148 (83.11%), Query Frame = 1


Query: 5   MASKRILKELKDLQKDPPTSCSAG-----------------------GVFLVNIHFPPDY 64
           MASKRILKELKDLQKDPPTSCSAG                       GVFLV IHFPPDY
Sbjct: 1   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVTIHFPPDY 60

Query: 65  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 124
           PFKPPKVAFRTKVFHPN+NSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV
Sbjct: 61  PFKPPKVAFRTKVFHPNVNSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120

Query: 125 PEIAHMYKTDRTKYEASARSWTQKYAMG 130
           PEIAHMYKTDR KYE++ARSWTQKYAMG
Sbjct: 121 PEIAHMYKTDRAKYESTARSWTQKYAMG 148

[Close]

BLAST of Lsi01G016270.1 vs. Swiss-Prot
Match: UBC10_ARATH (Ubiquitin-conjugating enzyme E2 10 OS=Arabidopsis thaliana GN=UBC10 PE=1 SV=1)

HSP 1 Score: 243.0 bits (619), Expect = 1.7e-63
Identity = 120/148 (81.08%), Postives = 123/148 (83.11%), Query Frame = 1


Query: 5   MASKRILKELKDLQKDPPTSCSAG-----------------------GVFLVNIHFPPDY 64
           MASKRILKELKDLQKDPPTSCSAG                       GVFLV IHFPPDY
Sbjct: 1   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSESPYAGGVFLVTIHFPPDY 60

Query: 65  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 124
           PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV
Sbjct: 61  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120

Query: 125 PEIAHMYKTDRTKYEASARSWTQKYAMG 130
           PEIAHMYKTD+ KYE++ARSWTQKYAMG
Sbjct: 121 PEIAHMYKTDKNKYESTARSWTQKYAMG 148

[Close]

BLAST of Lsi01G016270.1 vs. Swiss-Prot
Match: UBC9_ARATH (SUMO-conjugating enzyme UBC9 OS=Arabidopsis thaliana GN=UBC9 PE=1 SV=1)

HSP 1 Score: 241.9 bits (616), Expect = 3.9e-63
Identity = 119/148 (80.41%), Postives = 123/148 (83.11%), Query Frame = 1


Query: 5   MASKRILKELKDLQKDPPTSCSAG-----------------------GVFLVNIHFPPDY 64
           MASKRILKELKDLQKDPPTSCSAG                       GVFLV IHFPPDY
Sbjct: 1   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVTIHFPPDY 60

Query: 65  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 124
           PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV
Sbjct: 61  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120

Query: 125 PEIAHMYKTDRTKYEASARSWTQKYAMG 130
           PEIAHMYKTD+ KYE++AR+WTQKYAMG
Sbjct: 121 PEIAHMYKTDKNKYESTARTWTQKYAMG 148

[Close]

BLAST of Lsi01G016270.1 vs. TrEMBL
Match: A0A077S4E7_WHEAT (Uncharacterized protein OS=Triticum aestivum GN=TRAES_3BF006600070CFD_c1 PE=3 SV=1)

HSP 1 Score: 251.5 bits (641), Expect = 5.5e-64
Identity = 120/129 (93.02%), Postives = 124/129 (96.12%), Query Frame = 1


Query: 5   MASKRILKELKDLQKDPPTSCSAG----GVFLVNIHFPPDYPFKPPKVAFRTKVFHPNIN 64
           MASKRILKELKDLQKDPPTSCSAG    GVFLVNIHFPPDYPFKPPKV+F+TKVFHPNIN
Sbjct: 1   MASKRILKELKDLQKDPPTSCSAGPSGEGVFLVNIHFPPDYPFKPPKVSFKTKVFHPNIN 60

Query: 65  SNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKTDRTKYEASAR 124
           SNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKTDR+KYE +AR
Sbjct: 61  SNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKTDRSKYETTAR 120

Query: 125 SWTQKYAMG 130
           SWTQKYAMG
Sbjct: 121 SWTQKYAMG 129

[Close]

BLAST of Lsi01G016270.1 vs. TrEMBL
Match: A0A0E0JQC5_ORYPU (Uncharacterized protein OS=Oryza punctata PE=3 SV=1)

HSP 1 Score: 250.4 bits (638), Expect = 1.2e-63
Identity = 120/130 (92.31%), Postives = 123/130 (94.62%), Query Frame = 1


Query: 5   MASKRILKELKDLQKDPPTSCSA-----GGVFLVNIHFPPDYPFKPPKVAFRTKVFHPNI 64
           MASKRILKELKDLQKDPPTSCSA     GGVFLVNIHFPPDYPFKPPKV+F+TKVFHPNI
Sbjct: 1   MASKRILKELKDLQKDPPTSCSADSPYAGGVFLVNIHFPPDYPFKPPKVSFKTKVFHPNI 60

Query: 65  NSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKTDRTKYEASA 124
           NSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKTDR KYE +A
Sbjct: 61  NSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKTDRPKYETTA 120

Query: 125 RSWTQKYAMG 130
           RSWTQKYAMG
Sbjct: 121 RSWTQKYAMG 130

[Close]

BLAST of Lsi01G016270.1 vs. TrEMBL
Match: A0A0A0L2X5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G334720 PE=3 SV=1)

HSP 1 Score: 248.8 bits (634), Expect = 3.5e-63
Identity = 124/148 (83.78%), Postives = 124/148 (83.78%), Query Frame = 1


Query: 5   MASKRILKELKDLQKDPPTSCSA-----------------------GGVFLVNIHFPPDY 64
           MASKRILKELKDLQKDPPTSCSA                       GGVFLVNIHFPPDY
Sbjct: 1   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVNIHFPPDY 60

Query: 65  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 124
           PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV
Sbjct: 61  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120

Query: 125 PEIAHMYKTDRTKYEASARSWTQKYAMG 130
           PEIAHMYKTDR KYEASARSWTQKYAMG
Sbjct: 121 PEIAHMYKTDRAKYEASARSWTQKYAMG 148

[Close]

BLAST of Lsi01G016270.1 vs. TrEMBL
Match: A0A0A0KKF5_CUCSA (Ubiquitin carrier protein OS=Cucumis sativus GN=Csa_5G151540 PE=3 SV=1)

HSP 1 Score: 248.1 bits (632), Expect = 6.0e-63
Identity = 123/148 (83.11%), Postives = 125/148 (84.46%), Query Frame = 1


Query: 5   MASKRILKELKDLQKDPPTSCSA-----------------------GGVFLVNIHFPPDY 64
           MASKRILKELKDLQKDPPTSCSA                       GGVFLVNIHFPPDY
Sbjct: 1   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPPDSPYAGGVFLVNIHFPPDY 60

Query: 65  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 124
           PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV
Sbjct: 61  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120

Query: 125 PEIAHMYKTDRTKYEASARSWTQKYAMG 130
           PEIAHMYKTDR+KYEA+ARSWTQKYAMG
Sbjct: 121 PEIAHMYKTDRSKYEATARSWTQKYAMG 148

[Close]

BLAST of Lsi01G016270.1 vs. TrEMBL
Match: A0A0A0KEB9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G133760 PE=3 SV=1)

HSP 1 Score: 247.7 bits (631), Expect = 7.9e-63
Identity = 123/148 (83.11%), Postives = 125/148 (84.46%), Query Frame = 1


Query: 5   MASKRILKELKDLQKDPPTSCSAG-----------------------GVFLVNIHFPPDY 64
           MASKRILKELKDLQKDPPTSCSAG                       GVFLV+IHFPPDY
Sbjct: 1   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPADSPYSGGVFLVSIHFPPDY 60

Query: 65  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 124
           PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV
Sbjct: 61  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120

Query: 125 PEIAHMYKTDRTKYEASARSWTQKYAMG 130
           PEIAHMYKTDRTKYEA+ARSWTQKYAMG
Sbjct: 121 PEIAHMYKTDRTKYEATARSWTQKYAMG 148

[Close]

BLAST of Lsi01G016270.1 vs. TAIR10
Match: AT5G53300.1 (AT5G53300.1 ubiquitin-conjugating enzyme 10)

HSP 1 Score: 243.0 bits (619), Expect = 9.8e-65
Identity = 120/148 (81.08%), Postives = 123/148 (83.11%), Query Frame = 1


Query: 5   MASKRILKELKDLQKDPPTSCSAG-----------------------GVFLVNIHFPPDY 64
           MASKRILKELKDLQKDPPTSCSAG                       GVFLV IHFPPDY
Sbjct: 1   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSESPYAGGVFLVTIHFPPDY 60

Query: 65  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 124
           PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV
Sbjct: 61  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120

Query: 125 PEIAHMYKTDRTKYEASARSWTQKYAMG 130
           PEIAHMYKTD+ KYE++ARSWTQKYAMG
Sbjct: 121 PEIAHMYKTDKNKYESTARSWTQKYAMG 148

[Close]

BLAST of Lsi01G016270.1 vs. TAIR10
Match: AT4G27960.2 (AT4G27960.2 ubiquitin conjugating enzyme 9)

HSP 1 Score: 241.9 bits (616), Expect = 2.2e-64
Identity = 119/148 (80.41%), Postives = 123/148 (83.11%), Query Frame = 1


Query: 5   MASKRILKELKDLQKDPPTSCSAG-----------------------GVFLVNIHFPPDY 64
           MASKRILKELKDLQKDPPTSCSAG                       GVFLV IHFPPDY
Sbjct: 31  MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVTIHFPPDY 90

Query: 65  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 124
           PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV
Sbjct: 91  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 150

Query: 125 PEIAHMYKTDRTKYEASARSWTQKYAMG 130
           PEIAHMYKTD+ KYE++AR+WTQKYAMG
Sbjct: 151 PEIAHMYKTDKNKYESTARTWTQKYAMG 178

[Close]

BLAST of Lsi01G016270.1 vs. TAIR10
Match: AT5G41700.4 (AT5G41700.4 ubiquitin conjugating enzyme 8)

HSP 1 Score: 240.4 bits (612), Expect = 6.4e-64
Identity = 119/149 (79.87%), Postives = 122/149 (81.88%), Query Frame = 1


Query: 5   MASKRILKELKDLQKDPPTSCS------------------------AGGVFLVNIHFPPD 64
           MASKRILKELKDLQKDPPTSC                         +GGVFLV IHFPPD
Sbjct: 1   MASKRILKELKDLQKDPPTSCIFAGPVAEDMFHWQATIMGPAESPYSGGVFLVTIHFPPD 60

Query: 65  YPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPL 124
           YPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPL
Sbjct: 61  YPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPL 120

Query: 125 VPEIAHMYKTDRTKYEASARSWTQKYAMG 130
           VPEIAHMYKTDR KYEA+AR+WTQKYAMG
Sbjct: 121 VPEIAHMYKTDRAKYEATARNWTQKYAMG 149

[Close]

BLAST of Lsi01G016270.1 vs. TAIR10
Match: AT3G08690.1 (AT3G08690.1 ubiquitin-conjugating enzyme 11)

HSP 1 Score: 238.8 bits (608), Expect = 1.9e-63
Identity = 116/148 (78.38%), Postives = 125/148 (84.46%), Query Frame = 1


Query: 5   MASKRILKELKDLQKDPPTSCSAG-----------------------GVFLVNIHFPPDY 64
           MASKRILKELKDLQKDPP++CSAG                       GVFLV+IHFPPDY
Sbjct: 1   MASKRILKELKDLQKDPPSNCSAGPVAEDMFHWQATIMGPPESPYAGGVFLVSIHFPPDY 60

Query: 65  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 124
           PFKPPKV+F+TKV+HPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV
Sbjct: 61  PFKPPKVSFKTKVYHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120

Query: 125 PEIAHMYKTDRTKYEASARSWTQKYAMG 130
           PEIAHMYKTDR+KYE++ARSWTQKYAMG
Sbjct: 121 PEIAHMYKTDRSKYESTARSWTQKYAMG 148

[Close]

BLAST of Lsi01G016270.1 vs. TAIR10
Match: AT5G56150.1 (AT5G56150.1 ubiquitin-conjugating enzyme 30)

HSP 1 Score: 232.6 bits (592), Expect = 1.3e-61
Identity = 113/148 (76.35%), Postives = 121/148 (81.76%), Query Frame = 1


Query: 5   MASKRILKELKDLQKDPPTSCSAG-----------------------GVFLVNIHFPPDY 64
           MASKRI KEL+DLQ+DPP SCSAG                       GVFLV IHFPPDY
Sbjct: 1   MASKRINKELRDLQRDPPVSCSAGPTGDDMFQWQATIMGPADSPFAGGVFLVTIHFPPDY 60

Query: 65  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 124
           PFKPPKVAFRTKV+HPNINSNGSICLDILKEQWSPALT+SKVLLSICSLLTDPNPDDPLV
Sbjct: 61  PFKPPKVAFRTKVYHPNINSNGSICLDILKEQWSPALTVSKVLLSICSLLTDPNPDDPLV 120

Query: 125 PEIAHMYKTDRTKYEASARSWTQKYAMG 130
           PEIAH+YKTDR KYE++A+SWTQKYAMG
Sbjct: 121 PEIAHIYKTDRVKYESTAQSWTQKYAMG 148

[Close]

BLAST of Lsi01G016270.1 vs. NCBI nr
Match: gi|669030285|emb|CDM84761.1| (unnamed protein product [Triticum aestivum])

HSP 1 Score: 251.5 bits (641), Expect = 7.8e-64
Identity = 120/129 (93.02%), Postives = 124/129 (96.12%), Query Frame = 1


Query: 5   MASKRILKELKDLQKDPPTSCSAG----GVFLVNIHFPPDYPFKPPKVAFRTKVFHPNIN 64
           MASKRILKELKDLQKDPPTSCSAG    GVFLVNIHFPPDYPFKPPKV+F+TKVFHPNIN
Sbjct: 1   MASKRILKELKDLQKDPPTSCSAGPSGEGVFLVNIHFPPDYPFKPPKVSFKTKVFHPNIN 60

Query: 65  SNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKTDRTKYEASAR 124
           SNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKTDR+KYE +AR
Sbjct: 61  SNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKTDRSKYETTAR 120

Query: 125 SWTQKYAMG 130
           SWTQKYAMG
Sbjct: 121 SWTQKYAMG 129

[Close]

BLAST of Lsi01G016270.1 vs. NCBI nr
Match: gi|974994276|pdb|4X57|A (Chain A, Structure Of An Arabidopsis E2 / Membrane-anchored Ubiquitin-fold Protein Complex)

HSP 1 Score: 248.8 bits (634), Expect = 5.1e-63
Identity = 123/151 (81.46%), Postives = 125/151 (82.78%), Query Frame = 1


Query: 2   PFIMASKRILKELKDLQKDPPTSCSAG-----------------------GVFLVNIHFP 61
           PF MASKRILKELKDLQKDPPTSCSAG                       GVFLV IHFP
Sbjct: 29  PFTMASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPAESPYSGGVFLVTIHFP 88

Query: 62  PDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDD 121
           PDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDD
Sbjct: 89  PDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDD 148

Query: 122 PLVPEIAHMYKTDRTKYEASARSWTQKYAMG 130
           PLVPEIAHMYKTDR KYEA+AR+WTQKYAMG
Sbjct: 149 PLVPEIAHMYKTDRAKYEATARNWTQKYAMG 179

[Close]

BLAST of Lsi01G016270.1 vs. NCBI nr
Match: gi|449449847|ref|XP_004142676.1| (PREDICTED: ubiquitin-conjugating enzyme E2 28 [Cucumis sativus])

HSP 1 Score: 248.8 bits (634), Expect = 5.1e-63
Identity = 124/148 (83.78%), Postives = 124/148 (83.78%), Query Frame = 1


Query: 5   MASKRILKELKDLQKDPPTSCSA-----------------------GGVFLVNIHFPPDY 64
           MASKRILKELKDLQKDPPTSCSA                       GGVFLVNIHFPPDY
Sbjct: 1   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVNIHFPPDY 60

Query: 65  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 124
           PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV
Sbjct: 61  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120

Query: 125 PEIAHMYKTDRTKYEASARSWTQKYAMG 130
           PEIAHMYKTDR KYEASARSWTQKYAMG
Sbjct: 121 PEIAHMYKTDRAKYEASARSWTQKYAMG 148

[Close]

BLAST of Lsi01G016270.1 vs. NCBI nr
Match: gi|778699419|ref|XP_011654707.1| (PREDICTED: ubiquitin-conjugating enzyme E2-17 kDa [Cucumis sativus])

HSP 1 Score: 248.1 bits (632), Expect = 8.6e-63
Identity = 123/148 (83.11%), Postives = 125/148 (84.46%), Query Frame = 1


Query: 5   MASKRILKELKDLQKDPPTSCSA-----------------------GGVFLVNIHFPPDY 64
           MASKRILKELKDLQKDPPTSCSA                       GGVFLVNIHFPPDY
Sbjct: 1   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPPDSPYAGGVFLVNIHFPPDY 60

Query: 65  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 124
           PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV
Sbjct: 61  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120

Query: 125 PEIAHMYKTDRTKYEASARSWTQKYAMG 130
           PEIAHMYKTDR+KYEA+ARSWTQKYAMG
Sbjct: 121 PEIAHMYKTDRSKYEATARSWTQKYAMG 148

[Close]

BLAST of Lsi01G016270.1 vs. NCBI nr
Match: gi|659074001|ref|XP_008437368.1| (PREDICTED: ubiquitin-conjugating enzyme E2-17 kDa [Cucumis melo])

HSP 1 Score: 248.1 bits (632), Expect = 8.6e-63
Identity = 123/148 (83.11%), Postives = 125/148 (84.46%), Query Frame = 1


Query: 5   MASKRILKELKDLQKDPPTSCSA-----------------------GGVFLVNIHFPPDY 64
           MASKRILKELKDLQKDPPTSCSA                       GGVFLVNIHFPPDY
Sbjct: 91  MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPPDSPYAGGVFLVNIHFPPDY 150

Query: 65  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 124
           PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV
Sbjct: 151 PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 210

Query: 125 PEIAHMYKTDRTKYEASARSWTQKYAMG 130
           PEIAHMYKTDR+KYEA+ARSWTQKYAMG
Sbjct: 211 PEIAHMYKTDRSKYEATARSWTQKYAMG 238

The following BLAST results are available for this feature:

BLAST of Lsi01G016270.1 vs. Swiss-Prot


Match Name	E-value	Identity	Description
UBC8_ARATH	7.8e-64	81.76	Ubiquitin-conjugating enzyme E2 8 OS=Arabidopsis thaliana GN=UBC8 PE=1 SV=1	[more]
UBC4_SOLLC	7.8e-64	81.76	Ubiquitin-conjugating enzyme E2-17 kDa OS=Solanum lycopersicum PE=2 SV=1	[more]
UBC28_ARATH	1.0e-63	81.08	Ubiquitin-conjugating enzyme E2 28 OS=Arabidopsis thaliana GN=UBC28 PE=2 SV=1	[more]
UBC10_ARATH	1.7e-63	81.08	Ubiquitin-conjugating enzyme E2 10 OS=Arabidopsis thaliana GN=UBC10 PE=1 SV=1	[more]
UBC9_ARATH	3.9e-63	80.41	SUMO-conjugating enzyme UBC9 OS=Arabidopsis thaliana GN=UBC9 PE=1 SV=1	[more]

BLAST of Lsi01G016270.1 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A077S4E7_WHEAT	5.5e-64	93.02	Uncharacterized protein OS=Triticum aestivum GN=TRAES_3BF006600070CFD_c1 PE=3 SV...	[more]
A0A0E0JQC5_ORYPU	1.2e-63	92.31	Uncharacterized protein OS=Oryza punctata PE=3 SV=1	[more]
A0A0A0L2X5_CUCSA	3.5e-63	83.78	Uncharacterized protein OS=Cucumis sativus GN=Csa_4G334720 PE=3 SV=1	[more]
A0A0A0KKF5_CUCSA	6.0e-63	83.11	Ubiquitin carrier protein OS=Cucumis sativus GN=Csa_5G151540 PE=3 SV=1	[more]
A0A0A0KEB9_CUCSA	7.9e-63	83.11	Uncharacterized protein OS=Cucumis sativus GN=Csa_6G133760 PE=3 SV=1	[more]

BLAST of Lsi01G016270.1 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G53300.1	9.8e-65	81.08	ubiquitin-conjugating enzyme 10	[more]
AT4G27960.2	2.2e-64	80.41	ubiquitin conjugating enzyme 9	[more]
AT5G41700.4	6.4e-64	79.87	ubiquitin conjugating enzyme 8	[more]
AT3G08690.1	1.9e-63	78.38	ubiquitin-conjugating enzyme 11	[more]
AT5G56150.1	1.3e-61	76.35	ubiquitin-conjugating enzyme 30	[more]

BLAST of Lsi01G016270.1 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|669030285\|emb\|CDM84761.1\|	7.8e-64	93.02	unnamed protein product [Triticum aestivum]	[more]
gi\|974994276\|pdb\|4X57\|A	5.1e-63	81.46	Chain A, Structure Of An Arabidopsis E2 / Membrane-anchored Ubiquitin-fold Prote...	[more]
gi\|449449847\|ref\|XP_004142676.1\|	5.1e-63	83.78	PREDICTED: ubiquitin-conjugating enzyme E2 28 [Cucumis sativus]	[more]
gi\|778699419\|ref\|XP_011654707.1\|	8.6e-63	83.11	PREDICTED: ubiquitin-conjugating enzyme E2-17 kDa [Cucumis sativus]	[more]
gi\|659074001\|ref\|XP_008437368.1\|	8.6e-63	83.11	PREDICTED: ubiquitin-conjugating enzyme E2-17 kDa [Cucumis melo]	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **INTERPRO**
Term	Definition
IPR023313	UBQ-conjugating_AS
IPR016135	UBQ-conjugating_enzyme/RWD
IPR000608	UBQ-conjugat_E2

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0016874	ligase activity

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Lsi01G016270	Lsi01G016270	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Lsi01G016270.1	Lsi01G016270.1-protein	polypeptide

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lsi01G016270.1.three_prime_UTR.1	Lsi01G016270.1.three_prime_UTR.1	three_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lsi01G016270.1.CDS.5	Lsi01G016270.1.CDS.5	CDS
Lsi01G016270.1.CDS.4	Lsi01G016270.1.CDS.4	CDS
Lsi01G016270.1.CDS.3	Lsi01G016270.1.CDS.3	CDS
Lsi01G016270.1.CDS.2	Lsi01G016270.1.CDS.2	CDS
Lsi01G016270.1.CDS.1	Lsi01G016270.1.CDS.1	CDS

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000608	Ubiquitin-conjugating enzyme E2	PFAM	PF00179	UQ_con	coord: 12..122 score: 8.8
IPR000608	Ubiquitin-conjugating enzyme E2	PROFILE	PS50127	UBIQUITIN_CONJUGAT_2	coord: 21..117 score: 35
IPR016135	Ubiquitin-conjugating enzyme/RWD-like	GENE3D	G3DSA:3.10.110.10		coord: 5..27 score: 1.6E-4coord: 28..128 score: 5.2
IPR016135	Ubiquitin-conjugating enzyme/RWD-like	unknown	SSF54495	UBC-like	coord: 4..128 score: 2.95
IPR023313	Ubiquitin-conjugating enzyme, active site	PROSITE	PS00183	UBIQUITIN_CONJUGAT_1	coord: 55..70 scor
None	No IPR available	PANTHER	PTHR24068	FAMILY NOT NAMED	coord: 4..128 score: 1.3E
None	No IPR available	SMART	SM00212	ubc_7	coord: 8..128 score: 3.7