Cucsa.313450.2 (mRNA) Cucumber (Gy14) v1

Overview
NameCucsa.313450.2
TypemRNA
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionTobamovirus multiplication protein 2B
Locationscaffold02995 : 2625163 .. 2626182 (-)
Sequence length512
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GAAGCTTCAGGCGACGCCAACAATGGCGACAGCTTCCCCATCAGGTCGAAGCAACAGGGATGGAACAGCAAAAGCCATGGTTGCCGACCACATTTCTCAGTCTGTACGATCCACGTCAAATCTCCTTCATCTAATGCAGCAATCGTCTTCAGCTCAGGCATCCTCTCTCTCTCTCTCTCGTTTGTGTTTTCTGAAGCTTCACGAGTTCATTCGAACGGTTTGATGATGATCTTGATCATATGAATAGATGATTGAATTTCCAAATCTTAATTGTTCGTTTTGTATCTTTTTCCATTTTCTAGCTTCAGTTAATTGAGAAATCACGTTATGTTTCGAGGAAAATTGTGTAATATTGTACGAACTTTGACTGTGCTTGAATTGTTTGCATCGAAGTCATAATTTCAAGTTTTCACTACCTTGGACAATTGAAGTGAAAGTTACCTCTGATTTTTAGATGGATTCAACTCTTGGAAGTTTTAGTCATATTGCTGAAGTGCTATTTTGTTTGAGATTTTTTGTTATTGCATTGCTTTCAGATTTTCGGTTCTGTCTTGTCTGCAGGCTCAGTTAACAAAGTTGCCAAAGAATCTTTTGGCGAAAGCATCTACTATGAAAAATACTGGGAAAGTATGTAGCATCAGTTAATAACTTCAGCATGTTCTTCTTTCGTTTATTTATTTCTTATCAATAAGACTTCTGATAGTACTTTCACTTCTGTTCTTGTGATGCAGATCTTAGAGCAGATGCCGCAAGTGGTTTCATCATTAGATGCATATGTTGAGAAGGGACTAGAAAGGTTCCTTTTTTACCTTGAACATGCATTGGTGGAATTAGTTCTATTTCTATGTTCAAATGTATAAAGCACATGGGTTATTTCTTTGGTGAAGAAACCAATGATTTTCGTTAGATTTGATGACAGTTATTGAAGTGCATAAGTAAATGCTTTGTTTTCCAGTGATATATTTATATTAAAGAACTTTCATGTTGAAATTGATACGTCTGAGACGGGAGGGGAGGCTT

mRNA sequence

GAAGCTTCAGGCGACGCCAACAATGGCGACAGCTTCCCCATCAGGTCGAAGCAACAGGGATGGAACAGCAAAAGCCATGGTTGCCGACCACATTTCTCAGTCTGTACGATCCACGTCAAATCTCCTTCATCTAATGCAGCAATCGTCTTCAGCTCAGGCTCAGTTAACAAAGTTGCCAAAGAATCTTTTGGCGAAAGCATCTACTATGAAAAATACTGGGAAAATCTTAGAGCAGATGCCGCAAGTGGTTTCATCATTAGATGCATATGTTGAGAAGGGACTAGAAAGGTTCCTTTTTTACCTTGAACATGCATTGGTGGAATTAGTTCTATTTCTATGTTCAAATGTATAAAGCACATGGGTTATTTCTTTGGTGAAGAAACCAATGATTTTCGTTAGATTTGATGACAGTTATTGAAGTGCATAAGTAAATGCTTTGTTTTCCAGTGATATATTTATATTAAAGAACTTTCATGTTgaaattgatacgtctgagacgggaggggaggctt

Coding sequence (CDS)

ATGGCGACAGCTTCCCCATCAGGTCGAAGCAACAGGGATGGAACAGCAAAAGCCATGGTTGCCGACCACATTTCTCAGTCTGTACGATCCACGTCAAATCTCCTTCATCTAATGCAGCAATCGTCTTCAGCTCAGGCTCAGTTAACAAAGTTGCCAAAGAATCTTTTGGCGAAAGCATCTACTATGAAAAATACTGGGAAAATCTTAGAGCAGATGCCGCAAGTGGTTTCATCATTAGATGCATATGTTGAGAAGGGACTAGAAAGGTTCCTTTTTTACCTTGAACATGCATTGGTGGAATTAGTTCTATTTCTATGTTCAAATGTATAA

Protein sequence

MATASPSGRSNRDGTAKAMVADHISQSVRSTSNLLHLMQQSSSAQAQLTKLPKNLLAKASTMKNTGKILEQMPQVVSSLDAYVEKGLERFLFYLEHALVELVLFLCSNV*
Homology
[Close]
BLAST of Cucsa.313450.2 vs. Swiss-Prot
Match: TOM2B_ARATH (Tobamovirus multiplication protein 2B OS=Arabidopsis thaliana GN=TOM2B PE=2 SV=2)

HSP 1 Score: 114.0 bits (284), Expect = 1.0e-24
Identity = 62/89 (69.66%), Postives = 73/89 (82.02%), Query Frame = 1 

Query: 1  MATASPSGR--SNRDGTAKAMVADHISQSVRSTSNLLHLMQQSSSAQAQLTKLPKNLLAK 60
          MATAS + R  S  D TAKA+VAD ISQ+V ST+NLLHLM+QSSS+QAQL KLPKNLLAK
Sbjct: 1  MATASENSRGISGGDRTAKAVVADQISQAVNSTANLLHLMRQSSSSQAQLAKLPKNLLAK 60

Query: 61 ASTMKNTGKILEQMPQVVSSLDAYVEKGL 88
          AS  K TG+ L Q+PQV+SSLDA++E GL
Sbjct: 61 ASLTKATGQALAQLPQVISSLDAHIESGL 89

[Close]
BLAST of Cucsa.313450.2 vs. TrEMBL
Match: A0A0A0LI80_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G904110 PE=4 SV=1)

HSP 1 Score: 168.3 bits (425), Expect = 5.2e-39
Identity = 88/88 (100.00%), Postives = 88/88 (100.00%), Query Frame = 1 

Query: 1  MATASPSGRSNRDGTAKAMVADHISQSVRSTSNLLHLMQQSSSAQAQLTKLPKNLLAKAS 60
          MATASPSGRSNRDGTAKAMVADHISQSVRSTSNLLHLMQQSSSAQAQLTKLPKNLLAKAS
Sbjct: 1  MATASPSGRSNRDGTAKAMVADHISQSVRSTSNLLHLMQQSSSAQAQLTKLPKNLLAKAS 60

Query: 61 TMKNTGKILEQMPQVVSSLDAYVEKGLE 89
          TMKNTGKILEQMPQVVSSLDAYVEKGLE
Sbjct: 61 TMKNTGKILEQMPQVVSSLDAYVEKGLE 88

[Close]
BLAST of Cucsa.313450.2 vs. TrEMBL
Match: V4VCD3_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10033051mg PE=4 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 1.9e-25
Identity = 63/87 (72.41%), Postives = 77/87 (88.51%), Query Frame = 1 

Query: 3  TASPSGRSN-RDGTAKAMVADHISQSVRSTSNLLHLMQQSSSAQAQLTKLPKNLLAKAST 62
          TA+ SG S+ R+GTAKAMV D ISQ+++STSNLLHLMQQSS +Q QLTKLPKNLLAK + 
Sbjct: 5  TAAASGSSSSREGTAKAMVFDQISQTIQSTSNLLHLMQQSSPSQVQLTKLPKNLLAKTTM 64

Query: 63 MKNTGKILEQMPQVVSSLDAYVEKGLE 89
          +KNTG++LEQMPQV+SSLDA+VE GL+
Sbjct: 65 IKNTGQVLEQMPQVISSLDAHVENGLQ 91

[Close]
BLAST of Cucsa.313450.2 vs. TrEMBL
Match: U5GN76_POPTR (Tobamovirus multiplication protein 2B OS=Populus trichocarpa GN=POPTR_0001s02840g PE=4 SV=1)

HSP 1 Score: 118.6 bits (296), Expect = 4.7e-24
Identity = 58/87 (66.67%), Postives = 77/87 (88.51%), Query Frame = 1 

Query: 2   ATASPSGRSNRDGTAKAMVADHISQSVRSTSNLLHLMQQSSSAQAQLTKLPKNLLAKAST 61
           + +S    S R+GTAK++VAD ISQ+V+STSNLLHLMQQSS +QA+L KLPKNLLAKAST
Sbjct: 18  SASSGGSASAREGTAKSVVADQISQAVQSTSNLLHLMQQSSPSQAKLMKLPKNLLAKAST 77

Query: 62  MKNTGKILEQMPQVVSSLDAYVEKGLE 89
           +KNTG++L+QMP+V+SSLDA+++ GL+
Sbjct: 78  IKNTGQVLDQMPKVISSLDAHMDSGLQ 104

[Close]
BLAST of Cucsa.313450.2 vs. TrEMBL
Match: A0A0K9RR13_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_038950 PE=4 SV=1)

HSP 1 Score: 117.9 bits (294), Expect = 8.0e-24
Identity = 59/87 (67.82%), Postives = 70/87 (80.46%), Query Frame = 1 

Query: 1  MATASPSGRSNRDGTAKAMVADHISQSVRSTSNLLHLMQQSSSAQAQLTKLPKNLLAKAS 60
          MAT    GRS R+ TAK  VAD ISQS++STSNLL LMQQSS +QA L KLPKNLLAK  
Sbjct: 1  MATVGGRGRSTRESTAKTAVADQISQSIQSTSNLLQLMQQSSPSQAHLAKLPKNLLAKTD 60

Query: 61 TMKNTGKILEQMPQVVSSLDAYVEKGL 88
          T+KNTG++L+Q+P V+SSLDAY+E GL
Sbjct: 61 TIKNTGQVLQQLPLVISSLDAYMENGL 87

[Close]
BLAST of Cucsa.313450.2 vs. TrEMBL
Match: A0A0J8CDT0_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_5g106530 PE=4 SV=1)

HSP 1 Score: 116.3 bits (290), Expect = 2.3e-23
Identity = 58/87 (66.67%), Postives = 70/87 (80.46%), Query Frame = 1 

Query: 1  MATASPSGRSNRDGTAKAMVADHISQSVRSTSNLLHLMQQSSSAQAQLTKLPKNLLAKAS 60
          MAT    G S R+GTAKA VAD ISQS++STSNLL LMQQSS +Q  L KLPKNLLAK  
Sbjct: 1  MATVGGRGGSTREGTAKASVADQISQSIQSTSNLLQLMQQSSPSQGHLAKLPKNLLAKTD 60

Query: 61 TMKNTGKILEQMPQVVSSLDAYVEKGL 88
          T++NTG++L+Q+P V+SSLDAY+E GL
Sbjct: 61 TIRNTGQVLQQLPLVISSLDAYMENGL 87

[Close]
BLAST of Cucsa.313450.2 vs. TAIR10
Match: AT1G32370.2 (AT1G32370.2 tobamovirus multiplication 2B)

HSP 1 Score: 114.0 bits (284), Expect = 5.9e-26
Identity = 62/89 (69.66%), Postives = 73/89 (82.02%), Query Frame = 1 

Query: 1  MATASPSGR--SNRDGTAKAMVADHISQSVRSTSNLLHLMQQSSSAQAQLTKLPKNLLAK 60
          MATAS + R  S  D TAKA+VAD ISQ+V ST+NLLHLM+QSSS+QAQL KLPKNLLAK
Sbjct: 1  MATASENSRGISGGDRTAKAVVADQISQAVNSTANLLHLMRQSSSSQAQLAKLPKNLLAK 60

Query: 61 ASTMKNTGKILEQMPQVVSSLDAYVEKGL 88
          AS  K TG+ L Q+PQV+SSLDA++E GL
Sbjct: 61 ASLTKATGQALAQLPQVISSLDAHIESGL 89

[Close]
BLAST of Cucsa.313450.2 vs. NCBI nr
Match: gi|778688029|ref|XP_011652663.1| (PREDICTED: tobamovirus multiplication protein 2B [Cucumis sativus])

HSP 1 Score: 168.3 bits (425), Expect = 7.4e-39
Identity = 88/88 (100.00%), Postives = 88/88 (100.00%), Query Frame = 1 

Query: 1  MATASPSGRSNRDGTAKAMVADHISQSVRSTSNLLHLMQQSSSAQAQLTKLPKNLLAKAS 60
          MATASPSGRSNRDGTAKAMVADHISQSVRSTSNLLHLMQQSSSAQAQLTKLPKNLLAKAS
Sbjct: 1  MATASPSGRSNRDGTAKAMVADHISQSVRSTSNLLHLMQQSSSAQAQLTKLPKNLLAKAS 60

Query: 61 TMKNTGKILEQMPQVVSSLDAYVEKGLE 89
          TMKNTGKILEQMPQVVSSLDAYVEKGLE
Sbjct: 61 TMKNTGKILEQMPQVVSSLDAYVEKGLE 88

[Close]
BLAST of Cucsa.313450.2 vs. NCBI nr
Match: gi|659131950|ref|XP_008465939.1| (PREDICTED: tobamovirus multiplication protein 2B [Cucumis melo])

HSP 1 Score: 164.9 bits (416), Expect = 8.2e-38
Identity = 86/88 (97.73%), Postives = 87/88 (98.86%), Query Frame = 1 

Query: 1  MATASPSGRSNRDGTAKAMVADHISQSVRSTSNLLHLMQQSSSAQAQLTKLPKNLLAKAS 60
          MATASPSGRSNRDGTAKAMVADHISQSVRSTSNLLHLMQQSSSAQAQL KLPKNLLAKAS
Sbjct: 1  MATASPSGRSNRDGTAKAMVADHISQSVRSTSNLLHLMQQSSSAQAQLRKLPKNLLAKAS 60

Query: 61 TMKNTGKILEQMPQVVSSLDAYVEKGLE 89
          TM+NTGKILEQMPQVVSSLDAYVEKGLE
Sbjct: 61 TMRNTGKILEQMPQVVSSLDAYVEKGLE 88

[Close]
BLAST of Cucsa.313450.2 vs. NCBI nr
Match: gi|802659494|ref|XP_012080848.1| (PREDICTED: tobamovirus multiplication protein 2B isoform X1 [Jatropha curcas])

HSP 1 Score: 129.4 bits (324), Expect = 3.8e-27
Identity = 71/101 (70.30%), Postives = 82/101 (81.19%), Query Frame = 1 

Query: 1   MATASPSG-------------RSNRDGTAKAMVADHISQSVRSTSNLLHLMQQSSSAQAQ 60
           MAT+SPSG             RS R+ TAKAMVAD ISQ+V+STSNLL+LMQQSS++QAQ
Sbjct: 1   MATSSPSGGSGSGSGSGIPRSRSTREDTAKAMVADQISQAVQSTSNLLNLMQQSSASQAQ 60

Query: 61  LTKLPKNLLAKASTMKNTGKILEQMPQVVSSLDAYVEKGLE 89
           L KLPKNLLAKASTMKNTG+ LEQMPQV+SSLDA++E GLE
Sbjct: 61  LMKLPKNLLAKASTMKNTGQALEQMPQVISSLDAHMENGLE 101

[Close]
BLAST of Cucsa.313450.2 vs. NCBI nr
Match: gi|802659512|ref|XP_012080851.1| (PREDICTED: tobamovirus multiplication protein 2B isoform X2 [Jatropha curcas])

HSP 1 Score: 129.4 bits (324), Expect = 3.8e-27
Identity = 71/101 (70.30%), Postives = 82/101 (81.19%), Query Frame = 1 

Query: 1   MATASPSG-------------RSNRDGTAKAMVADHISQSVRSTSNLLHLMQQSSSAQAQ 60
           MAT+SPSG             RS R+ TAKAMVAD ISQ+V+STSNLL+LMQQSS++QAQ
Sbjct: 1   MATSSPSGGSGSGSGSGIPRSRSTREDTAKAMVADQISQAVQSTSNLLNLMQQSSASQAQ 60

Query: 61  LTKLPKNLLAKASTMKNTGKILEQMPQVVSSLDAYVEKGLE 89
           L KLPKNLLAKASTMKNTG+ LEQMPQV+SSLDA++E GLE
Sbjct: 61  LMKLPKNLLAKASTMKNTGQALEQMPQVISSLDAHMENGLE 101

[Close]
BLAST of Cucsa.313450.2 vs. NCBI nr
Match: gi|731381208|ref|XP_010664032.1| (PREDICTED: tobamovirus multiplication protein 2B isoform X2 [Vitis vinifera])

HSP 1 Score: 126.7 bits (317), Expect = 2.5e-26
Identity = 64/90 (71.11%), Postives = 77/90 (85.56%), Query Frame = 1 

Query: 3   TASPSGRSNRDGTAKAMVADHISQSVRSTSNLLHLMQQSSSAQAQLTKLPKNLLAKASTM 62
           +AS    S R+ T KA +AD ISQSV+STSNLLHLMQQSS + AQLTKLPKNLLAK ST+
Sbjct: 15  SASAGRGSARESTVKATMADQISQSVQSTSNLLHLMQQSSPSHAQLTKLPKNLLAKTSTI 74

Query: 63  KNTGKILEQMPQVVSSLDAYVEKGLERFLF 93
           KNTG++LEQMP+V+SSLDA++E GL+RFLF
Sbjct: 75  KNTGQLLEQMPRVISSLDAHIESGLQRFLF 104

The following BLAST results are available for this feature:
BLAST of Cucsa.313450.2 vs. Swiss-Prot
Match NameE-valueIdentityDescription
TOM2B_ARATH1.0e-2469.66Tobamovirus multiplication protein 2B OS=Arabidopsis thaliana GN=TOM2B PE=2 SV=2[more]
BLAST of Cucsa.313450.2 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LI80_CUCSA5.2e-39100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_3G904110 PE=4 SV=1[more]
V4VCD3_9ROSI1.9e-2572.41Uncharacterized protein OS=Citrus clementina GN=CICLE_v10033051mg PE=4 SV=1[more]
U5GN76_POPTR4.7e-2466.67Tobamovirus multiplication protein 2B OS=Populus trichocarpa GN=POPTR_0001s02840... [more]
A0A0K9RR13_SPIOL8.0e-2467.82Uncharacterized protein OS=Spinacia oleracea GN=SOVF_038950 PE=4 SV=1[more]
A0A0J8CDT0_BETVU2.3e-2366.67Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_5g106530 PE=4 S... [more]
BLAST of Cucsa.313450.2 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G32370.25.9e-2669.66 tobamovirus multiplication 2B[more]
BLAST of Cucsa.313450.2 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|778688029|ref|XP_011652663.1|7.4e-39100.00PREDICTED: tobamovirus multiplication protein 2B [Cucumis sativus][more]
gi|659131950|ref|XP_008465939.1|8.2e-3897.73PREDICTED: tobamovirus multiplication protein 2B [Cucumis melo][more]
gi|802659494|ref|XP_012080848.1|3.8e-2770.30PREDICTED: tobamovirus multiplication protein 2B isoform X1 [Jatropha curcas][more]
gi|802659512|ref|XP_012080851.1|3.8e-2770.30PREDICTED: tobamovirus multiplication protein 2B isoform X2 [Jatropha curcas][more]
gi|731381208|ref|XP_010664032.1|2.5e-2671.11PREDICTED: tobamovirus multiplication protein 2B isoform X2 [Vitis vinifera][more]
GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0046786 viral replication complex formation and maintenance
cellular_component GO:0005575 cellular_component
molecular_function GO:0003674 molecular_function
Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Cucsa.313450Cucsa.313450gene


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Cucsa.313450.2Cucsa.313450.2-proteinpolypeptide


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsa.313450.2.CDS.3Cucsa.313450.2.CDS.3CDS
Cucsa.313450.2.CDS.2Cucsa.313450.2.CDS.2CDS
Cucsa.313450.2.CDS.1Cucsa.313450.2.CDS.1CDS


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsa.313450.2.three_prime_UTR.1Cucsa.313450.2.three_prime_UTR.1three_prime_UTR


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsa.313450.2.five_prime_UTR.1Cucsa.313450.2.five_prime_UTR.1five_prime_UTR