Cucsa.303110.3 (mRNA) Cucumber (Gy14) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cucsa.303110.3
Type	mRNA
Organism	Cucumis sativus (Cucumber (Gy14) v1)
Description	Polynucleotidyl transferase isoform 1
Location	scaffold02951 : 1081476 .. 1084414 (-)
Sequence length	945

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAGGATTCGGGCTTGATGGAGTCTTGACTCACATTCGATTGCGAATGAGTCTGAATGTGCATAACCGATTGTACGTTTTGATCCTCAAAGGCGCGAAGCTGATAATGGTTTGTATGAATTAAATAGCAATACGAGATGAAGTATGTGAGGCCGTTGATGCTTTTTCGTGATTTGACGAAGAGAGATGCACTTGAACATGGCCGTTTGCTTGGTTTAGATGTTGGTGACAAGTATGTTGGTTTAGCCGTGTCTGACCCAGATAATAAAATCGCTTGCCCTCTCAGGTATTTTGTTCATGATGGGATTTTGTGTGAGGGATTGGGAGTAATGGGGCTGAAGCTGAAGTTAACCAAACTTGACCAGTGTTAGTTGTTGTCGATTGTTGCAGTGTTTTGTTACGGAAGAAGACCACAATGGATTTGATGGCTCAGGATTTTCAGAAAATGGTGAGGCCTTTTACTTTGGTTCTTTTTACTTTGTCGTTCCTATTAAGATTTGTGGAATGTAATTTGATGCAATGCTGAGTGATAACAATGATTGAAGGTTGGTGCCACAAAATCTTGTCGGGAGAACTTTAATTTACTTGAGATATTATTTTCTCTTTCTTTTATCTTTTTCCGTCGTGTGAAGATTTGTTCAAGGTTATTTGATGCTATGCTGAATGATAAACGATGACTGGTTGTGCCACAAAAATCTATAGGGAGTATTTTTTTTAAAAAATCTATTTCCTAGGAAGATTTGTAGAACGATATTTGATGAAATGCTGAATGATAGACAATGATTGAAGGTTGAACAAAATTTTGCAGGGAGAACTTTAATTTTCTTGAGATTTTCTTCTCTCTTTATATCTATCTATCTATCTCTACACACACGCAATCTTTTTCCTTCCTATGAAGATTGGTAGAATGTTGTTTTATGCAATCCTAAATGATAAACAATGATTGAAGGGTGGTGCACACAAATTTTCAGGGAGAATTTTTTCGGTGAGTACCACTTTAGGTGTTGAAATGGGATTTATTAATGATGACACCAGGATTACTTTGTAGTTGCATAGCTTTAACTTTACCTTTTTCCTTACCTCATTTCCTGAGATGTGTTGTTGCGCAACGTATTTGCCTTTAAACTTCAATATATGATAGTGAATTGTTAATGTTGTTCTTTCTTGCAACATTCAGTCATCTCCATCCTGTTTGTATTTACAGTTGTTTCTGATTTGTGTATTCAGATCTCTGAATTTTCCCTGGCGGGGTTCATTGTTGGGTACCCTTTCGATAGACTACGGAACAACCCGGACGTGCGTTCTTTATCTCTTCAGAGACATCATTATTGACTCTTATCTGTACTTGACCTTATATTGTTCTGAGTACAGGCTATGCAAGTGAAGATCTTCATTGATGACCTTTGTAAAACTGGAAAACTTGAAGGCGTAAAGTATACTTTTTGGGATGAGTGCTTTACATCAAAGGTTATTGCATCTAGTTTAGCAAGCCCTTCCGGTTCGTCTTGTGACATGTGGCATGCCTTTCAATGTCTCGGTCCATGTCTCCGTTGAATTCACAAACTTGGTCAAATGAGTGTGATTATGTTTTGAATTGTCTTTCACCTGATAAAATTTTCTTTTACGTGGTGCTGACTTATGCATAGGCTACTGCTACATTGAATTTCATCAACAATGAATTTTATATTTGTGATTTACTGATTTCTTTTATCTATACACAGAATGTGGAACTACTGATAAAGCCATTAAACCTGCTCCCCGCTGTGTCAAAGACAATAATTGACAAATTTGCTGCTGTTGGAATACTTCAGGTAATTTAGAATTCTCTCTCTCTTTTGCTTAAACTTTCATCTTGATAAGGTTGGCAGCTGAAATGATTATCCAATTTTCTCAATGTAAGAATAATATCTTAAAATATCAATAGAAGCTTCAGTGTATTAACAATAAGGTGTGTATGTCAGGGTTACTTGGATTATTTTAACAGAAGACCGGAATTGGGCGAGACGTAGAGTAAGGTGGACTATATGGGATCAGGTACTTGTTTGTCAACGGAGTTGCTTTCTTTGCAAATTGAAATTTCAAAATTTTGAATTAAAAAATGTCCTTTTGTTCATAAATGTTTACCGTGACTAGAAAATTGGGTTTTCAAATTTAGTTTCTAATGTAAATTTTAGACCATTTAGAAACTAATTTCATATTTCATGGACTAGTCAGAAACTGCATTTGTACTGGATCCAACATGTCATTTTTGGATTTCTAAGGACTAAATAGAACTAAAAGTATTAGTTTTGAAATTCATATACCAAATAAAAATTAAGGGGTTGTTTGGACCCTCAACTTTGAGTGGGTGGAGTGGTGTATTATAATACACTCCATGTTTGGGTCTCGAATAACAATAGAGTGGTTCTTTTTAACTAACCTTAAAATAATTTACACTCTAAATATAAAGTATTATAAAGTATCGACTAATAACTAACTCAACGTTTCAAATGTTTTAAGTTTAAGTATCACCCATGAAGGTTTTATTTTATTACCTAGGTCGGTGATATTTGTGATTTCTAAAGGACAGTTTGTGTTAAACTTTGCCTTCAAATTTTAGGCTTCCTTGTATTGTTCCCACCCTTTGATAATAATTTGATACATTATACATTGATCATTATATGCAGACATTTGATTCTCCCTTCTTAGTAGGGGATGGAACAATTTATTTGAAGCTCTCAGTTCTTAAGTGGCGATGAATATGCATTGACAAATGACATCTTAAAAGGGTGAATCATCTGCTCTCAACCGACTAATCAGCTAATGGGGTTCGTTCTTCTTTATGTAGACTTTTTCTTGTGTGAATTCCATCAAGTGCTTGTTTCCATTTTTGTTTATTACTAAATTATTACTAATGACAACAACTGTTCAATATAGTTTGTCTATATTACAAATCAACCTC

mRNA sequence

AAGGATTCGGGCTTGATGGAGTCTTGACTCACATTCGATTGCGAATGAGTCTGAATGTGCATAACCGATTGTACGTTTTGATCCTCAAAGGCGCGAAGCTGATAATGGTTTGTATGAATTAAATAGCAATACGAGATGAAGTATGTGAGGCCGTTGATGCTTTTTCGTGATTTGACGAAGAGAGATGCACTTGAACATGGCCGTTTGCTTGGTTTAGATGTTGGTGACAAGTATGTTGGTTTAGCCGTGTCTGACCCAGATAATAAAATCGCTTGCCCTCTCAGTGTTTTGTTACGGAAGAAGACCACAATGGATTTGATGGCTCAGGATTTTCAGAAAATGATCTCTGAATTTTCCCTGGCGGGGTTCATTGTTGGGTACCCTTTCGATAGACTACGGAACAACCCGGACGCTATGCAAGTGAAGATCTTCATTGATGACCTTTGTAAAACTGGAAAACTTGAAGGCGTAAAGTATACTTTTTGGGATGAGTGCTTTACATCAAAGAATGTGGAACTACTGATAAAGCCATTAAACCTGCTCCCCGCTGTGTCAAAGACAATAATTGACAAATTTGCTGCTGTTGGAATACTTCAGGGTTACTTGGATTATTTTAACAGAAGACCGGAATTGGGCGAGACGTAGAGTAAGGTGGACTATATGGGATCAGACATTTGATTCTCCCTTCTTAGTAGGGGATGGAACAATTTATTTGAAGCTCTCAGTTCTTAAGTGGCGATGAATATGCATTGACAAATGACATCTTAAAAGGGTGAATCATCTGCTCTCAACCGACTAATCAGCTAATGGGGTTCGTTCTTCTTTATGTAGACTTTTTCTTGTGTGAATTCCATCAAGTGCTTGTTTCCATTTTTGTTTATTACTAAATTATTACTAATGACAACAACTGTTCAATATAGTTTGTCTATATTACAAATCAACCTC

Coding sequence (CDS)

ATGAAGTATGTGAGGCCGTTGATGCTTTTTCGTGATTTGACGAAGAGAGATGCACTTGAACATGGCCGTTTGCTTGGTTTAGATGTTGGTGACAAGTATGTTGGTTTAGCCGTGTCTGACCCAGATAATAAAATCGCTTGCCCTCTCAGTGTTTTGTTACGGAAGAAGACCACAATGGATTTGATGGCTCAGGATTTTCAGAAAATGATCTCTGAATTTTCCCTGGCGGGGTTCATTGTTGGGTACCCTTTCGATAGACTACGGAACAACCCGGACGCTATGCAAGTGAAGATCTTCATTGATGACCTTTGTAAAACTGGAAAACTTGAAGGCGTAAAGTATACTTTTTGGGATGAGTGCTTTACATCAAAGAATGTGGAACTACTGATAAAGCCATTAAACCTGCTCCCCGCTGTGTCAAAGACAATAATTGACAAATTTGCTGCTGTTGGAATACTTCAGGGTTACTTGGATTATTTTAACAGAAGACCGGAATTGGGCGAGACGTAG

Protein sequence

MKYVRPLMLFRDLTKRDALEHGRLLGLDVGDKYVGLAVSDPDNKIACPLSVLLRKKTTMDLMAQDFQKMISEFSLAGFIVGYPFDRLRNNPDAMQVKIFIDDLCKTGKLEGVKYTFWDECFTSKNVELLIKPLNLLPAVSKTIIDKFAAVGILQGYLDYFNRRPELGET*

Homology

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BLAST of Cucsa.303110.3 vs. Swiss-Prot
Match: YQGF_CALS8 (Putative pre-16S rRNA nuclease OS=Caldicellulosiruptor saccharolyticus (strain ATCC 43494 / DSM 8903 / Tp8T 6331) GN=Csac_1776 PE=3 SV=1)

HSP 1 Score: 77.8 bits (190), Expect = 1.3e-13
Identity = 51/145 (35.17%), Postives = 79/145 (54.48%), Query Frame = 1


Query: 23  RLLGLDVGDKYVGLAVSDPDNKIACPLSVLLRKKTTMDLMAQDF----QKMISEFSLAGF 82
           R+L LD+G + +G+A+SDP    A P  VL       DL  +D      K+ +E  +   
Sbjct: 2   RILCLDIGSRRIGVAISDPLKIAAQPFCVL-------DLQKEDLYSCLDKIFNEHQIEKI 61

Query: 83  IVGYPFDRLRNNPDAMQVKIF-IDDLC-KTGKLEGVKYTFWDECFTSKNVELLIKPLNLL 142
           ++GYP  +   +PD    K+  ID++C +  +   V    WDE F++K VE +++  N+ 
Sbjct: 62  VIGYPVSKF--HPDKATEKLQKIDEICSELERRYKVGIIKWDERFSTKAVERILREENVS 121

Query: 143 PAVSKTIIDKFAAVGILQGYLDYFN 162
               K ++DK AAV ILQGYLD+ N
Sbjct: 122 WQKRKKVVDKLAAVYILQGYLDFIN 137

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BLAST of Cucsa.303110.3 vs. Swiss-Prot
Match: YQGF_RHOCS (Putative pre-16S rRNA nuclease OS=Rhodospirillum centenum (strain ATCC 51521 / SW) GN=RC1_1518 PE=3 SV=1)

HSP 1 Score: 74.3 bits (181), Expect = 1.4e-12
Identity = 48/141 (34.04%), Postives = 75/141 (53.19%), Query Frame = 1


Query: 21  HGRLLGLDVGDKYVGLAVSDPDNKIACPLSVLLRKKTTMDLMAQDFQKMISEFSLAGFIV 80
           +GRL+GLD+G+K +GLAVSDP   +A P+  + R K T D  AQ+  + + +  +   +V
Sbjct: 16  NGRLMGLDLGEKTIGLAVSDPGLSVASPVGTIRRTKFTQD--AQELARAMRDRDVRALVV 75

Query: 81  GYPFDR-LRNNPDAMQVKIFIDDLCKTGKLEGV--KYTFWDECFTSKNVELLIKPLNLLP 140
           G P +      P    V+ F   L +  +L G   +  FWDE  ++  VE ++   ++  
Sbjct: 76  GLPVNMDGTEGPRCQSVREFSRLLLQRPQLFGFEPEIAFWDERLSTSAVERMMIGWDMTR 135

Query: 141 AVSKTIIDKFAAVGILQGYLD 159
                ++DK AA  ILQG LD
Sbjct: 136 KRRDEVVDKMAAAYILQGALD 154

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BLAST of Cucsa.303110.3 vs. Swiss-Prot
Match: YQGF_CARHZ (Putative pre-16S rRNA nuclease OS=Carboxydothermus hydrogenoformans (strain ATCC BAA-161 / DSM 6008 / Z-2901) GN=CHY_0542 PE=3 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 2.4e-12
Identity = 50/138 (36.23%), Postives = 74/138 (53.62%), Query Frame = 1


Query: 23  RLLGLDVGDKYVGLAVSDPDNKIACPLSVLLRKKTTMDLMAQDFQKMISEFSLAGFIVGY 82
           ++LGLDVGDK +G+A+SD     A  L+V+ R     DL  ++ +K+I    +   ++GY
Sbjct: 2   KVLGLDVGDKKIGVALSDELGLTAQGLTVIYRTSLKKDL--EEIKKIIDLHHVTEVVIGY 61

Query: 83  PFDRLRN-NPDAMQVKIFIDDLCKTGKLEGVKYTFWDECFTSKNVELLIKPLNLLPAVSK 142
           P +      P A  VK F  +L K     G+K  FWDE  ++   E L+   ++     K
Sbjct: 62  PRNMNGTAGPRAELVKRFARELYK---FTGIKPVFWDERLSTVEAEKLLISGDISRRKRK 121

Query: 143 TIIDKFAAVGILQGYLDY 160
            +IDK AA  IL GYL+Y
Sbjct: 122 KVIDKLAATLILSGYLNY 134

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BLAST of Cucsa.303110.3 vs. Swiss-Prot
Match: YQGF_NITHX (Putative pre-16S rRNA nuclease OS=Nitrobacter hamburgensis (strain X14 / DSM 10229) GN=Nham_2279 PE=3 SV=2)

HSP 1 Score: 73.6 bits (179), Expect = 2.4e-12
Identity = 49/140 (35.00%), Postives = 74/140 (52.86%), Query Frame = 1


Query: 20  EHGRLLGLDVGDKYVGLAVSDPDNKIACPLSVLLRKKTTMDLMAQDFQKMISEFSLAGFI 79
           E G L+GLD+G K +G+AVSDPD ++A  +  L RK  T D  A     +  E + AGF+
Sbjct: 17  ERGALVGLDLGTKTIGVAVSDPDRRLATGVETLQRKTFTAD--AARLLAIAGERNAAGFV 76

Query: 80  VGYPFDR-LRNNPDAMQVKIFIDDLCKTGKLEGVKYTFWDECFTSKNVELLIKPLNLLPA 139
           +G P +      P A   + F  +L    +L  +    WDE  ++  VE  +  +++  A
Sbjct: 77  LGLPINMDGSEGPRAQSTRAFARNL---ARLTDLAIGLWDERLSTVAVERELIGMDMSRA 136

Query: 140 VSKTIIDKFAAVGILQGYLD 159
               +ID+ AA+ ILQG LD
Sbjct: 137 KRAKVIDEHAAIFILQGALD 151

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BLAST of Cucsa.303110.3 vs. Swiss-Prot
Match: YQGF_SYNWW (Putative pre-16S rRNA nuclease OS=Syntrophomonas wolfei subsp. wolfei (strain DSM 2245B / Goettingen) GN=Swol_0470 PE=3 SV=1)

HSP 1 Score: 72.8 bits (177), Expect = 4.1e-12
Identity = 49/137 (35.77%), Postives = 73/137 (53.28%), Query Frame = 1


Query: 23  RLLGLDVGDKYVGLAVSDPDNKIACPLSVLLRKKTTMDLMAQDFQKMISEFSLAGFIVGY 82
           R++GLD+G+K +G+A SDP    A   S+L RK    DL     Q++  EF +   ++G 
Sbjct: 2   RIMGLDLGEKRIGIAFSDPMGWTAQGHSILQRKGLKKDLSY--LQELCQEFQVEKIVLGL 61

Query: 83  PFDRLRN-NPDAMQVKIFIDDLCKTGKLEGVKYTFWDECFTSKNVELLIKPLNLLPAVSK 142
           P +      P A++ + F   L +  K   +   FWDE  +SK+ E ++   +L     K
Sbjct: 62  PLNMNGTMGPKALETQEFARALQEALK---IPVDFWDERLSSKSAERVLLEADLSRKRRK 121

Query: 143 TIIDKFAAVGILQGYLD 159
            +IDK AAV ILQ YLD
Sbjct: 122 ELIDKIAAVHILQAYLD 133

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BLAST of Cucsa.303110.3 vs. TrEMBL
Match: A0A0A0KMK7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G153680 PE=3 SV=1)

HSP 1 Score: 342.4 bits (877), Expect = 3.1e-91
Identity = 169/169 (100.00%), Postives = 169/169 (100.00%), Query Frame = 1


Query: 1   MKYVRPLMLFRDLTKRDALEHGRLLGLDVGDKYVGLAVSDPDNKIACPLSVLLRKKTTMD 60
           MKYVRPLMLFRDLTKRDALEHGRLLGLDVGDKYVGLAVSDPDNKIACPLSVLLRKKTTMD
Sbjct: 1   MKYVRPLMLFRDLTKRDALEHGRLLGLDVGDKYVGLAVSDPDNKIACPLSVLLRKKTTMD 60

Query: 61  LMAQDFQKMISEFSLAGFIVGYPFDRLRNNPDAMQVKIFIDDLCKTGKLEGVKYTFWDEC 120
           LMAQDFQKMISEFSLAGFIVGYPFDRLRNNPDAMQVKIFIDDLCKTGKLEGVKYTFWDEC
Sbjct: 61  LMAQDFQKMISEFSLAGFIVGYPFDRLRNNPDAMQVKIFIDDLCKTGKLEGVKYTFWDEC 120

Query: 121 FTSKNVELLIKPLNLLPAVSKTIIDKFAAVGILQGYLDYFNRRPELGET 170
           FTSKNVELLIKPLNLLPAVSKTIIDKFAAVGILQGYLDYFNRRPELGET
Sbjct: 121 FTSKNVELLIKPLNLLPAVSKTIIDKFAAVGILQGYLDYFNRRPELGET 169

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BLAST of Cucsa.303110.3 vs. TrEMBL
Match: D7TW01_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_02s0025g03570 PE=3 SV=1)

HSP 1 Score: 255.0 bits (650), Expect = 6.5e-65
Identity = 125/162 (77.16%), Postives = 141/162 (87.04%), Query Frame = 1


Query: 1   MKYVRPLMLFRDLTKRDALEHGRLLGLDVGDKYVGLAVSDPDNKIACPLSVLLRKKTTMD 60
           MKYV+P+ LF DL K  A E GRLLGLDVGDKYVGLAVSD  NKIA PLSVLLRKK+ +D
Sbjct: 56  MKYVKPVNLFHDLLKMKATERGRLLGLDVGDKYVGLAVSDLHNKIASPLSVLLRKKSNID 115

Query: 61  LMAQDFQKMISEFSLAGFIVGYPFDRLRNNPDAMQVKIFIDDLCKTGKLEGVKYTFWDEC 120
           LMA DFQ +IS+ SLAGFIVGYPF+R R++P A+ VK+FIDDLCKTGKLEGV+YT+WDEC
Sbjct: 116 LMATDFQSLISDLSLAGFIVGYPFNRQRSSPTAVNVKVFIDDLCKTGKLEGVRYTYWDEC 175

Query: 121 FTSKNVELLIKPLNLLPAVSKTIIDKFAAVGILQGYLDYFNR 163
           FTSKNVELL+KPLNL P  SKTI+DKFAAVGILQGYLD+ NR
Sbjct: 176 FTSKNVELLLKPLNLHPVQSKTILDKFAAVGILQGYLDHVNR 217

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BLAST of Cucsa.303110.3 vs. TrEMBL
Match: B9GKI3_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0001s09310g PE=3 SV=1)

HSP 1 Score: 255.0 bits (650), Expect = 6.5e-65
Identity = 123/166 (74.10%), Postives = 143/166 (86.14%), Query Frame = 1


Query: 1   MKYVRPLMLFRDLTKRDALEHGRLLGLDVGDKYVGLAVSDPDNKIACPLSVLLRKKTTMD 60
           MKYVRPL LF +L K  A+  GR LGLDVGDKYVGLAVSDP NKIA PLSVLLRKK+ ++
Sbjct: 1   MKYVRPLSLFHELLKTSAINQGRFLGLDVGDKYVGLAVSDPLNKIASPLSVLLRKKSNIE 60

Query: 61  LMAQDFQKMISEFSLAGFIVGYPFDRLRNNPDAMQVKIFIDDLCKTGKLEGVKYTFWDEC 120
           LMA DFQ +ISE SL GFIVGYPFDR R  PDA++VK+F+DDLCKTGKLEGVK+T+WDEC
Sbjct: 61  LMATDFQSLISELSLGGFIVGYPFDRQRGAPDAVRVKLFVDDLCKTGKLEGVKFTYWDEC 120

Query: 121 FTSKNVELLIKPLNLLPAVSKTIIDKFAAVGILQGYLDYFNRRPEL 167
           FTSKNVELL+KPL+L P  +K+I+DKFAAVGILQGYLDY N++ +L
Sbjct: 121 FTSKNVELLVKPLDLHPVHAKSIMDKFAAVGILQGYLDYVNKKMKL 166

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BLAST of Cucsa.303110.3 vs. TrEMBL
Match: A0A067JRJ0_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_20599 PE=3 SV=1)

HSP 1 Score: 253.8 bits (647), Expect = 1.4e-64
Identity = 125/169 (73.96%), Postives = 142/169 (84.02%), Query Frame = 1


Query: 1   MKYVRPLMLFRDLTKRDALEHGRLLGLDVGDKYVGLAVSDPDNKIACPLSVLLRKKTTMD 60
           MKYV+PL LF +L K  A    RLLGLDVG KYVGLA+SD  N+IA PLSVLLRKK+ +D
Sbjct: 1   MKYVKPLALFHELMKAKAQGERRLLGLDVGGKYVGLAISDQCNEIASPLSVLLRKKSNID 60

Query: 61  LMAQDFQKMISEFSLAGFIVGYPFDRLRNNPDAMQVKIFIDDLCKTGKLEGVKYTFWDEC 120
           LMA DFQ + SE SLAGF+VGYPFDRLR+ PDA+QVK+FIDDLCKTGKLEG+KYT+WDEC
Sbjct: 61  LMADDFQSLTSELSLAGFVVGYPFDRLRSAPDAVQVKVFIDDLCKTGKLEGLKYTYWDEC 120

Query: 121 FTSKNVELLIKPLNLLPAVSKTIIDKFAAVGILQGYLDYFNRRPELGET 170
           FTSKNVELLIKPL L P  +KT+IDKFAAVGILQGYLDY NR  ++ ET
Sbjct: 121 FTSKNVELLIKPLALCPTQAKTMIDKFAAVGILQGYLDYVNRETKMEET 169

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BLAST of Cucsa.303110.3 vs. TrEMBL
Match: A0A0J8C2Z4_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_6g145830 PE=3 SV=1)

HSP 1 Score: 253.8 bits (647), Expect = 1.4e-64
Identity = 122/162 (75.31%), Postives = 143/162 (88.27%), Query Frame = 1


Query: 1   MKYVRPLMLFRDLTKRDALEHGRLLGLDVGDKYVGLAVSDPDNKIACPLSVLLRKKTTMD 60
           MK+++PL LF+DL K +ALE GRLLGLDVGDKYVGLA+SDP+NKIA PLSVLLRKK  +D
Sbjct: 163 MKHMKPLSLFQDLLKTNALERGRLLGLDVGDKYVGLALSDPENKIATPLSVLLRKKFNID 222

Query: 61  LMAQDFQKMISEFSLAGFIVGYPFDRLRNNPDAMQVKIFIDDLCKTGKLEGVKYTFWDEC 120
           +MA DF  +ISEFSL+GFIVG P+DRLR +P A+QVK+F+DDLCKTGKLEGVKYT+W+EC
Sbjct: 223 MMAADFTSLISEFSLSGFIVGCPYDRLRPSPHAVQVKVFVDDLCKTGKLEGVKYTYWNEC 282

Query: 121 FTSKNVELLIKPLNLLPAVSKTIIDKFAAVGILQGYLDYFNR 163
           FTSKNVELL+KPL L P   KT++DKFAAVGILQGYLDY NR
Sbjct: 283 FTSKNVELLLKPLTLHPVELKTMLDKFAAVGILQGYLDYVNR 324

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BLAST of Cucsa.303110.3 vs. TAIR10
Match: AT3G52905.1 (AT3G52905.1 Polynucleotidyl transferase, ribonuclease H-like superfamily protein)

HSP 1 Score: 196.8 bits (499), Expect = 1.1e-50
Identity = 97/162 (59.88%), Postives = 120/162 (74.07%), Query Frame = 1


Query: 1   MKYVRPLMLFRDLTKRDALEHGRLLGLDVGDKYVGLAVSDPDNKIACPLSVLLRKKTTMD 60
           MKYV+PL L  +  K      GR LGLDVGDKYVGLA+SDP N +A PLSVLLRKK+ +D
Sbjct: 1   MKYVKPLSLLGNALKTKVSVPGRFLGLDVGDKYVGLAISDPSNMVASPLSVLLRKKSNID 60

Query: 61  LMAQDFQKMISEFSLAGFIVGYPFDRLRNNPDAMQVKIFIDDLCKTGKLEGVKYTFWDEC 120
           LMA DFQ ++  FS++G +VGYPF +L N  D + V +FI++L KT KL+ VKYT+WDE 
Sbjct: 61  LMATDFQNLVKAFSVSGLVVGYPFGKLNNVEDVVTVNLFIEELRKTEKLKDVKYTYWDER 120

Query: 121 FTSKNVELLIKPLNLLPAVSKTIIDKFAAVGILQGYLDYFNR 163
            +SK VEL++KPLNL P   KT++DK AAV ILQ YLDY NR
Sbjct: 121 LSSKTVELMLKPLNLHPVQEKTMLDKLAAVVILQEYLDYANR 162

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BLAST of Cucsa.303110.3 vs. NCBI nr
Match: gi|449452328|ref|XP_004143911.1| (PREDICTED: uncharacterized protein LOC101219574 [Cucumis sativus])

HSP 1 Score: 342.4 bits (877), Expect = 4.4e-91
Identity = 169/169 (100.00%), Postives = 169/169 (100.00%), Query Frame = 1


Query: 1   MKYVRPLMLFRDLTKRDALEHGRLLGLDVGDKYVGLAVSDPDNKIACPLSVLLRKKTTMD 60
           MKYVRPLMLFRDLTKRDALEHGRLLGLDVGDKYVGLAVSDPDNKIACPLSVLLRKKTTMD
Sbjct: 1   MKYVRPLMLFRDLTKRDALEHGRLLGLDVGDKYVGLAVSDPDNKIACPLSVLLRKKTTMD 60

Query: 61  LMAQDFQKMISEFSLAGFIVGYPFDRLRNNPDAMQVKIFIDDLCKTGKLEGVKYTFWDEC 120
           LMAQDFQKMISEFSLAGFIVGYPFDRLRNNPDAMQVKIFIDDLCKTGKLEGVKYTFWDEC
Sbjct: 61  LMAQDFQKMISEFSLAGFIVGYPFDRLRNNPDAMQVKIFIDDLCKTGKLEGVKYTFWDEC 120

Query: 121 FTSKNVELLIKPLNLLPAVSKTIIDKFAAVGILQGYLDYFNRRPELGET 170
           FTSKNVELLIKPLNLLPAVSKTIIDKFAAVGILQGYLDYFNRRPELGET
Sbjct: 121 FTSKNVELLIKPLNLLPAVSKTIIDKFAAVGILQGYLDYFNRRPELGET 169

[Close]

BLAST of Cucsa.303110.3 vs. NCBI nr
Match: gi|659073795|ref|XP_008437257.1| (PREDICTED: uncharacterized protein LOC103482742 isoform X1 [Cucumis melo])

HSP 1 Score: 300.8 bits (769), Expect = 1.5e-78
Identity = 148/169 (87.57%), Postives = 158/169 (93.49%), Query Frame = 1


Query: 1   MKYVRPLMLFRDLTKRDALEHGRLLGLDVGDKYVGLAVSDPDNKIACPLSVLLRKKTTMD 60
           MKYV+P +LFRDL KRD L+H RLLGLDVGDKYVGLAVSDPDNKIA PLSVLLRKK TMD
Sbjct: 1   MKYVKPSILFRDLLKRDELKHSRLLGLDVGDKYVGLAVSDPDNKIASPLSVLLRKKNTMD 60

Query: 61  LMAQDFQKMISEFSLAGFIVGYPFDRLRNNPDAMQVKIFIDDLCKTGKLEGVKYTFWDEC 120
           LMA+DFQK+ISEFSLAGFIVGYPFDRLR N +A+QVKIFIDDLCKTGKLEGVKYTFWDE 
Sbjct: 61  LMAEDFQKLISEFSLAGFIVGYPFDRLRKNTEAIQVKIFIDDLCKTGKLEGVKYTFWDEG 120

Query: 121 FTSKNVELLIKPLNLLPAVSKTIIDKFAAVGILQGYLDYFNRRPELGET 170
           FTSKNVE +IKPLNLLPAVSKT++DKFAAVGILQGYLDYFNRRPEL ET
Sbjct: 121 FTSKNVEQMIKPLNLLPAVSKTMLDKFAAVGILQGYLDYFNRRPELSET 169

[Close]

BLAST of Cucsa.303110.3 vs. NCBI nr
Match: gi|659073797|ref|XP_008437258.1| (PREDICTED: uncharacterized protein LOC103482742 isoform X2 [Cucumis melo])

HSP 1 Score: 270.8 bits (691), Expect = 1.6e-69
Identity = 134/154 (87.01%), Postives = 144/154 (93.51%), Query Frame = 1


Query: 1   MKYVRPLMLFRDLTKRDALEHGRLLGLDVGDKYVGLAVSDPDNKIACPLSVLLRKKTTMD 60
           MKYV+P +LFRDL KRD L+H RLLGLDVGDKYVGLAVSDPDNKIA PLSVLLRKK TMD
Sbjct: 1   MKYVKPSILFRDLLKRDELKHSRLLGLDVGDKYVGLAVSDPDNKIASPLSVLLRKKNTMD 60

Query: 61  LMAQDFQKMISEFSLAGFIVGYPFDRLRNNPDAMQVKIFIDDLCKTGKLEGVKYTFWDEC 120
           LMA+DFQK+ISEFSLAGFIVGYPFDRLR N +A+QVKIFIDDLCKTGKLEGVKYTFWDE 
Sbjct: 61  LMAEDFQKLISEFSLAGFIVGYPFDRLRKNTEAIQVKIFIDDLCKTGKLEGVKYTFWDEG 120

Query: 121 FTSKNVELLIKPLNLLPAVSKTIIDKFAAVGILQ 155
           FTSKNVE +IKPLNLLPAVSKT++DKFAAVGILQ
Sbjct: 121 FTSKNVEQMIKPLNLLPAVSKTMLDKFAAVGILQ 154

[Close]

BLAST of Cucsa.303110.3 vs. NCBI nr
Match: gi|743852442|ref|XP_011029263.1| (PREDICTED: uncharacterized protein LOC105129048 isoform X2 [Populus euphratica])

HSP 1 Score: 256.1 bits (653), Expect = 4.2e-65
Identity = 124/166 (74.70%), Postives = 143/166 (86.14%), Query Frame = 1


Query: 1   MKYVRPLMLFRDLTKRDALEHGRLLGLDVGDKYVGLAVSDPDNKIACPLSVLLRKKTTMD 60
           MKYVRPL LF +L K  A+  GR LGLDVGDKYVGLAVSDP NKIA PLSVLLRKK+ ++
Sbjct: 1   MKYVRPLSLFHELLKTSAINQGRFLGLDVGDKYVGLAVSDPLNKIASPLSVLLRKKSNIE 60

Query: 61  LMAQDFQKMISEFSLAGFIVGYPFDRLRNNPDAMQVKIFIDDLCKTGKLEGVKYTFWDEC 120
           LMA DFQ +ISE SL GFIVGYPFDR R  PDA++VK+F+DDLCKTGKLEGVK+T+WDEC
Sbjct: 61  LMATDFQSLISELSLGGFIVGYPFDRQRGAPDAVRVKLFVDDLCKTGKLEGVKFTYWDEC 120

Query: 121 FTSKNVELLIKPLNLLPAVSKTIIDKFAAVGILQGYLDYFNRRPEL 167
           FTSKNVELL+KPL+L P  +K+I+DKFAAVGILQGYLDY NR+ +L
Sbjct: 121 FTSKNVELLVKPLDLHPVHAKSIMDKFAAVGILQGYLDYVNRKMKL 166

[Close]

BLAST of Cucsa.303110.3 vs. NCBI nr
Match: gi|224053911|ref|XP_002298037.1| (hypothetical protein POPTR_0001s09310g [Populus trichocarpa])

HSP 1 Score: 255.0 bits (650), Expect = 9.3e-65
Identity = 123/166 (74.10%), Postives = 143/166 (86.14%), Query Frame = 1


Query: 1   MKYVRPLMLFRDLTKRDALEHGRLLGLDVGDKYVGLAVSDPDNKIACPLSVLLRKKTTMD 60
           MKYVRPL LF +L K  A+  GR LGLDVGDKYVGLAVSDP NKIA PLSVLLRKK+ ++
Sbjct: 1   MKYVRPLSLFHELLKTSAINQGRFLGLDVGDKYVGLAVSDPLNKIASPLSVLLRKKSNIE 60

Query: 61  LMAQDFQKMISEFSLAGFIVGYPFDRLRNNPDAMQVKIFIDDLCKTGKLEGVKYTFWDEC 120
           LMA DFQ +ISE SL GFIVGYPFDR R  PDA++VK+F+DDLCKTGKLEGVK+T+WDEC
Sbjct: 61  LMATDFQSLISELSLGGFIVGYPFDRQRGAPDAVRVKLFVDDLCKTGKLEGVKFTYWDEC 120

Query: 121 FTSKNVELLIKPLNLLPAVSKTIIDKFAAVGILQGYLDYFNRRPEL 167
           FTSKNVELL+KPL+L P  +K+I+DKFAAVGILQGYLDY N++ +L
Sbjct: 121 FTSKNVELLVKPLDLHPVHAKSIMDKFAAVGILQGYLDYVNKKMKL 166

The following BLAST results are available for this feature:

BLAST of Cucsa.303110.3 vs. Swiss-Prot


Match Name	E-value	Identity	Description
YQGF_CALS8	1.3e-13	35.17	Putative pre-16S rRNA nuclease OS=Caldicellulosiruptor saccharolyticus (strain A...	[more]
YQGF_RHOCS	1.4e-12	34.04	Putative pre-16S rRNA nuclease OS=Rhodospirillum centenum (strain ATCC 51521 / S...	[more]
YQGF_CARHZ	2.4e-12	36.23	Putative pre-16S rRNA nuclease OS=Carboxydothermus hydrogenoformans (strain ATCC...	[more]
YQGF_NITHX	2.4e-12	35.00	Putative pre-16S rRNA nuclease OS=Nitrobacter hamburgensis (strain X14 / DSM 102...	[more]
YQGF_SYNWW	4.1e-12	35.77	Putative pre-16S rRNA nuclease OS=Syntrophomonas wolfei subsp. wolfei (strain DS...	[more]

BLAST of Cucsa.303110.3 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KMK7_CUCSA	3.1e-91	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G153680 PE=3 SV=1	[more]
D7TW01_VITVI	6.5e-65	77.16	Putative uncharacterized protein OS=Vitis vinifera GN=VIT_02s0025g03570 PE=3 SV=...	[more]
B9GKI3_POPTR	6.5e-65	74.10	Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0001s09310g PE=3 SV=1	[more]
A0A067JRJ0_JATCU	1.4e-64	73.96	Uncharacterized protein OS=Jatropha curcas GN=JCGZ_20599 PE=3 SV=1	[more]
A0A0J8C2Z4_BETVU	1.4e-64	75.31	Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_6g145830 PE=3 S...	[more]

BLAST of Cucsa.303110.3 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G52905.1	1.1e-50	59.88	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]

BLAST of Cucsa.303110.3 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449452328\|ref\|XP_004143911.1\|	4.4e-91	100.00	PREDICTED: uncharacterized protein LOC101219574 [Cucumis sativus]	[more]
gi\|659073795\|ref\|XP_008437257.1\|	1.5e-78	87.57	PREDICTED: uncharacterized protein LOC103482742 isoform X1 [Cucumis melo]	[more]
gi\|659073797\|ref\|XP_008437258.1\|	1.6e-69	87.01	PREDICTED: uncharacterized protein LOC103482742 isoform X2 [Cucumis melo]	[more]
gi\|743852442\|ref\|XP_011029263.1\|	4.2e-65	74.70	PREDICTED: uncharacterized protein LOC105129048 isoform X2 [Populus euphratica]	[more]
gi\|224053911\|ref\|XP_002298037.1\|	9.3e-65	74.10	hypothetical protein POPTR_0001s09310g [Populus trichocarpa]	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **INTERPRO**
Term	Definition
IPR005227	YqgF
IPR006641	YqgF/RNaseH-like_dom
IPR012337	RNaseH-like_sf

Vocabulary: **Biological Process**
Term	Definition
GO:0006364	rRNA processing
GO:0006139	nucleobase-containing compound metabolic process

Vocabulary: **Molecular Function**
Term	Definition
GO:0003676	nucleic acid binding

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006139	nucleobase-containing compound metabolic process
biological_process	GO:0006364	rRNA processing
biological_process	GO:0006310	DNA recombination
biological_process	GO:0031564	transcription antitermination
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003676	nucleic acid binding

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsa.303110	Cucsa.303110	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsa.303110.3	Cucsa.303110.3-protein	polypeptide

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsa.303110.3.three_prime_UTR.2	Cucsa.303110.3.three_prime_UTR.2	three_prime_UTR
Cucsa.303110.3.three_prime_UTR.1	Cucsa.303110.3.three_prime_UTR.1	three_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsa.303110.3.CDS.6	Cucsa.303110.3.CDS.6	CDS
Cucsa.303110.3.CDS.5	Cucsa.303110.3.CDS.5	CDS
Cucsa.303110.3.CDS.4	Cucsa.303110.3.CDS.4	CDS
Cucsa.303110.3.CDS.3	Cucsa.303110.3.CDS.3	CDS
Cucsa.303110.3.CDS.2	Cucsa.303110.3.CDS.2	CDS
Cucsa.303110.3.CDS.1	Cucsa.303110.3.CDS.1	CDS

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsa.303110.3.five_prime_UTR.1	Cucsa.303110.3.five_prime_UTR.1	five_prime_UTR

InterPro

Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005227	Putative pre-16S rRNA nuclease	HAMAP	MF_00651	Nuclease_YqgF	coord: 22..161 score: 16
IPR005227	Putative pre-16S rRNA nuclease	PFAM	PF03652	RuvX	coord: 23..158 score: 1.3
IPR005227	Putative pre-16S rRNA nuclease	TIGRFAMs	TIGR00250	TIGR00250	coord: 25..158 score: 1.7
IPR006641	YqgF/RNase H-like domain	GENE3D	G3DSA:3.30.420.140		coord: 22..161 score: 3.4
IPR006641	YqgF/RNase H-like domain	SMART	SM00732	rnase_8s	coord: 22..126 score: 6.8
IPR012337	Ribonuclease H-like domain	unknown	SSF53098	Ribonuclease H-like	coord: 22..161 score: 2.28
None	No IPR available	PANTHER	PTHR33317	FAMILY NOT NAMED	coord: 1..163 score: 2.5
None	No IPR available	PANTHER	PTHR33317:SF1	POLYNUCLEOTIDYL TRANSFERASE/ RIBONUCLEASE H-LIKE PROTEIN	coord: 1..163 score: 2.5