Cucsa.302220.1 (mRNA) Cucumber (Gy14) v1

NameCucsa.302220.1
TypemRNA
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionCytochrome P450, putative
Locationscaffold02951 : 419901 .. 422326 (+)
Sequence length1579
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
GCCGACACTCTCAATTCCCTTCCTCTTCTCATTTTGGTCCTTAGATTTCAACAAACACAGTCAAATTTCAACAGCACTTCTTTTTTTCTTCCTTTTCATTTTTGCTCTCTTATGGCTCCAATCCAAATTCCAGCATCCCCCTTTGCCTCCAGGCCCCCGTGGCCTGCCCCTGCTCGGTTATCTTCCATTTTTATCCACCAATCTCCACCACACATTCAACAATTTGGCCAAAATGTACGGCCCAATTTTCAAGCTCCGCCTCGGAACTAAGCTCTGCGTTGTTCTCACCTCCCCTTCCTCCGTCAATGAAGTCCTCCGTCACCAAGAAACTGTCTTCGACAACCGAGATGCCACCGTCAGCGCTCTTCTTGCCACCTACGGCGGAGTCGATATTGTATTCAGCCAGAACGAGAGCATTTGGAAGAAGCTGAGAAAAATCTTTGCCCGAAAAATGCTTAGCAAATCAAATCTTGATGCGTCGTATCCTTTGCGAAGACGAGAAGTGAGAAAAATAATTAAAGGCGTGTTGGAATCGGCGGGAACCCCAATTGATATCAGTAAATTGAGTTTGATGGCTGCTGTGAAATCGGTAATGGCGATGACGTGGGGTGGCTCAAGGGGACTGATCGGAGTGGATGGGGCTGATTTGGAAGCGAAGTTCATGGAAGTGGTGAATGAACTGATGGTGTTGCTTGGAACTCCAAATTTGTCGGATCTATTTCCGGTGTTGGGTGGGTTAGATTTGCAGGGAATTGGGAGGAAGATGAAGAAGGTGATGAATGTTTTTGATGGGATTTTTAATTCTGCCATTGAAGAACAGAGGAAAAGGGGAGGAGATGGGATGGAAAACAGAGGGTTTTTACAGTTACTGTTGGAGGTTATGGAGGGTGAGGATAGTTCAGAGTCCATTACCGATAAAGAACTCAAGGCTTTGCTAGTCGTATGCTTTCCCAATTACTTATCTTTTCATTGCCATTCCAATATGCTCTTTTTTACCCTTAAATGATTATTAGATTCTATTTAAATCCATACCATTCCAATTTCTAAAAATTAGTTCAAAATTATAATTATAATGAGCGAAGTTGTTTTAAATTACAAATAAAAAaTTTAAAATATTTATAAATATGCCAAAaTTTTATATGTTATTAGTGATAAATAGGTATCGATAGATATATATATATATATATAAGTGATAAACGCTAATaTTCTATCATTATTATATTTTGTAAATATTTTAGTTTATTTtATTGTAATTtAAAaTATAATTTtAAAAACAATTtATTTTtCTTGTAAtTTATTtTTtAAAAAAAaTTGTGATAATGCAAGCTCTATCTGAGTGGAGCTATATTATTTTAGGACAAGTATACCTGCCTTTTCTTTTATCACTTCTAATAATATTTGACTTCTTAATCAAATTTAAAaTTTAAAATTTTAAAATATTGTCCTAGTTTAAAAAATTAGGTTTTTTTtCCTATAAAAaCAAATTGGTATGGTTTTGTTTGAGTTAAAATTTTACATTTGTGCCCAATCATTTTTTtCTCATAATGGAATCTCAATTATGGCAATTTTCAATTTATAATACATATTAAAAGTCATTCCTTCGAATAAATTTTTtGAACAAGCTCTTGATAACTGTTATGAGATAGAAaaaGATAGATGAGTATGAGGTTTTtCCCCcTTTTTTTTTTttCCTGTTCTTTTTCtCTAATTGATTTTTTtATTGAATTTACATTAAGTAGTTCAATGTATTTAAGAACCTATGATCTCTTTTTAATGGTTTTGTATAGGACATCATCATTGGAGGAACAGACACAACATCAACTACAATTGAGTGGACAATCGCAGAATTGATACAACAACCAAACATAATGAAGAAAGTCAAAGAAGAATTAACAAAAGTTGTGGGTTTAAACCAAATGGTTGAAGAATTTCACTTATCTAAATTACATTATTTAGATGCAGCAATTAAAGAAACACTTCGTTTACACCCACCAGTACCTCTTTTAGTGCCACGGACGACTAACCAAAGAACCACCCTCGAAGGGTACACTATTCCAAAGAGCTCAACTATCTACTTCAATATTTGGGCCATCCAAAGAGACCCTAAAATTTGGGACAACCCATTAGACTTCATGCCTGAGAGATTTTTGAATGAATCTAATGAAAATATGTACGATTTTACTGGCAATAAGATAGAGTTTTGTCCATTTGGAGCAGGTAAAAAGTTATGTGTGGGGATTCCTCTAGCCGAGAGGTTGTTGGTTTTAATATTAGCTTCATTGTTGCATGGATTTGAATGGAAATTACCCGAGGGTTCAACGCTTGATTTGGAAGAAAAGTTTGGAATTGTCAGTAAGAAGTTGAATCCTTTGGTTGTTATTCCTACACCAAGGCTTTCCAACTTGGAGCTTTATAATATGATGTAG

mRNA sequence

GCCGACACTCTCAATTCCCTTCCTCTTCTCATTTTGGTCCTTAGATTTCAACAAACACAGTCAAATTTCAACAGCACTTCTTTTTTTCTTCCTTTTCATTTTTGCTCTCTTATGGCTCCAATCCAAATTCCAGCATCCCCCTTTGCCTCCAGGCCCCCGTGGCCTGCCCCTGCTCGGTTATCTTCCATTTTTATCCACCAATCTCCACCACACATTCAACAATTTGGCCAAAATGTACGGCCCAATTTTCAAGCTCCGCCTCGGAACTAAGCTCTGCGTTGTTCTCACCTCCCCTTCCTCCGTCAATGAAGTCCTCCGTCACCAAGAAACTGTCTTCGACAACCGAGATGCCACCGTCAGCGCTCTTCTTGCCACCTACGGCGGAGTCGATATTGTATTCAGCCAGAACGAGAGCATTTGGAAGAAGCTGAGAAAAATCTTTGCCCGAAAAATGCTTAGCAAATCAAATCTTGATGCGTCGTATCCTTTGCGAAGACGAGAAGTGAGAAAAATAATTAAAGGCGTGTTGGAATCGGCGGGAACCCCAATTGATATCAGTAAATTGAGTTTGATGGCTGCTGTGAAATCGGTAATGGCGATGACGTGGGGTGGCTCAAGGGGACTGATCGGAGTGGATGGGGCTGATTTGGAAGCGAAGTTCATGGAAGTGGTGAATGAACTGATGGTGTTGCTTGGAACTCCAAATTTGTCGGATCTATTTCCGGTGTTGGGTGGGTTAGATTTGCAGGGAATTGGGAGGAAGATGAAGAAGGTGATGAATGTTTTTGATGGGATTTTTAATTCTGCCATTGAAGAACAGAGGAAAAGGGGAGGAGATGGGATGGAAAACAGAGGGTTTTTACAGTTACTGTTGGAGGTTATGGAGGGTGAGGATAGTTCAGAGTCCATTACCGATAAAGAACTCAAGGCTTTGCTAGTCGACATCATCATTGGAGGAACAGACACAACATCAACTACAATTGAGTGGACAATCGCAGAATTGATACAACAACCAAACATAATGAAGAAAGTCAAAGAAGAATTAACAAAAGTTGTGGGTTTAAACCAAATGGTTGAAGAATTTCACTTATCTAAATTACATTATTTAGATGCAGCAATTAAAGAAACACTTCGTTTACACCCACCAGTACCTCTTTTAGTGCCACGGACGACTAACCAAAGAACCACCCTCGAAGGGTACACTATTCCAAAGAGCTCAACTATCTACTTCAATATTTGGGCCATCCAAAGAGACCCTAAAATTTGGGACAACCCATTAGACTTCATGCCTGAGAGATTTTTGAATGAATCTAATGAAAATATGTACGATTTTACTGGCAATAAGATAGAGTTTTGTCCATTTGGAGCAGGTAAAAAGTTATGTGTGGGGATTCCTCTAGCCGAGAGGTTGTTGGTTTTAATATTAGCTTCATTGTTGCATGGATTTGAATGGAAATTACCCGAGGGTTCAACGCTTGATTTGGAAGAAAAGTTTGGAATTGTCAGTAAGAAGTTGAATCCTTTGGTTGTTATTCCTACACCAAGGCTTTCCAACTTGGAGCTTTATAATATGATGTAG

Coding sequence (CDS)

ATGTACGGCCCAATTTTCAAGCTCCGCCTCGGAACTAAGCTCTGCGTTGTTCTCACCTCCCCTTCCTCCGTCAATGAAGTCCTCCGTCACCAAGAAACTGTCTTCGACAACCGAGATGCCACCGTCAGCGCTCTTCTTGCCACCTACGGCGGAGTCGATATTGTATTCAGCCAGAACGAGAGCATTTGGAAGAAGCTGAGAAAAATCTTTGCCCGAAAAATGCTTAGCAAATCAAATCTTGATGCGTCGTATCCTTTGCGAAGACGAGAAGTGAGAAAAATAATTAAAGGCGTGTTGGAATCGGCGGGAACCCCAATTGATATCAGTAAATTGAGTTTGATGGCTGCTGTGAAATCGGTAATGGCGATGACGTGGGGTGGCTCAAGGGGACTGATCGGAGTGGATGGGGCTGATTTGGAAGCGAAGTTCATGGAAGTGGTGAATGAACTGATGGTGTTGCTTGGAACTCCAAATTTGTCGGATCTATTTCCGGTGTTGGGTGGGTTAGATTTGCAGGGAATTGGGAGGAAGATGAAGAAGGTGATGAATGTTTTTGATGGGATTTTTAATTCTGCCATTGAAGAACAGAGGAAAAGGGGAGGAGATGGGATGGAAAACAGAGGGTTTTTACAGTTACTGTTGGAGGTTATGGAGGGTGAGGATAGTTCAGAGTCCATTACCGATAAAGAACTCAAGGCTTTGCTAGTCGACATCATCATTGGAGGAACAGACACAACATCAACTACAATTGAGTGGACAATCGCAGAATTGATACAACAACCAAACATAATGAAGAAAGTCAAAGAAGAATTAACAAAAGTTGTGGGTTTAAACCAAATGGTTGAAGAATTTCACTTATCTAAATTACATTATTTAGATGCAGCAATTAAAGAAACACTTCGTTTACACCCACCAGTACCTCTTTTAGTGCCACGGACGACTAACCAAAGAACCACCCTCGAAGGGTACACTATTCCAAAGAGCTCAACTATCTACTTCAATATTTGGGCCATCCAAAGAGACCCTAAAATTTGGGACAACCCATTAGACTTCATGCCTGAGAGATTTTTGAATGAATCTAATGAAAATATGTACGATTTTACTGGCAATAAGATAGAGTTTTGTCCATTTGGAGCAGGTAAAAAGTTATGTGTGGGGATTCCTCTAGCCGAGAGGTTGTTGGTTTTAATATTAGCTTCATTGTTGCATGGATTTGAATGGAAATTACCCGAGGGTTCAACGCTTGATTTGGAAGAAAAGTTTGGAATTGTCAGTAAGAAGTTGAATCCTTTGGTTGTTATTCCTACACCAAGGCTTTCCAACTTGGAGCTTTATAATATGATGTAG

Protein sequence

MYGPIFKLRLGTKLCVVLTSPSSVNEVLRHQETVFDNRDATVSALLATYGGVDIVFSQNESIWKKLRKIFARKMLSKSNLDASYPLRRREVRKIIKGVLESAGTPIDISKLSLMAAVKSVMAMTWGGSRGLIGVDGADLEAKFMEVVNELMVLLGTPNLSDLFPVLGGLDLQGIGRKMKKVMNVFDGIFNSAIEEQRKRGGDGMENRGFLQLLLEVMEGEDSSESITDKELKALLVDIIIGGTDTTSTTIEWTIAELIQQPNIMKKVKEELTKVVGLNQMVEEFHLSKLHYLDAAIKETLRLHPPVPLLVPRTTNQRTTLEGYTIPKSSTIYFNIWAIQRDPKIWDNPLDFMPERFLNESNENMYDFTGNKIEFCPFGAGKKLCVGIPLAERLLVLILASLLHGFEWKLPEGSTLDLEEKFGIVSKKLNPLVVIPTPRLSNLELYNMM*
BLAST of Cucsa.302220.1 vs. Swiss-Prot
Match: F3PH_PETHY (Flavonoid 3'-monooxygenase OS=Petunia hybrida GN=CYP75B2 PE=2 SV=1)

HSP 1 Score: 298.5 bits (763), Expect = 1.2e-79
Identity = 163/445 (36.63%), Postives = 257/445 (57.75%), Query Frame = 1

Query: 2   YGPIFKLRLGTKLCVVLTSPSSVNEVLRHQETVFDNRDATVSALLATYGGVDIVFSQNES 61
           YGP+  L++G    VV  S S   + L+  +  F +R     A    Y   D+VF+    
Sbjct: 64  YGPLMYLKMGFVDVVVAASASVAAQFLKTHDANFSSRPPNSGAEHMAYNYQDLVFAPYGP 123

Query: 62  IWKKLRKIFARKMLSKSNLDASYPLRRREVRKIIKGVLESAGTPIDISKLSLMAAVKSVM 121
            W+ LRKI +  + S   LD    +R+ EV+ + + +  +   P+ + +L L     + +
Sbjct: 124 RWRMLRKICSVHLFSTKALDDFRHVRQDEVKTLTRALASAGQKPVKLGQL-LNVCTTNAL 183

Query: 122 AMTWGGSRGLIGVDGA-DLE---AKFMEVVNELMVLLGTPNLSDLFPVLGGLDLQGIGRK 181
           A    G R  +  DG+ D++   A+F  +V E+MV+ G  N+ D  P L  LD+QG+  K
Sbjct: 184 ARVMLGKR--VFADGSGDVDPQAAEFKSMVVEMMVVAGVFNIGDFIPQLNWLDIQGVAAK 243

Query: 182 MKKVMNVFDGIFNSAIEEQRKRGGDGMENRGFLQLLLEVMEGEDSSESITDKELKALLVD 241
           MKK+   FD      +EE + +    M++     + L+  + ++    +TD E+KALL++
Sbjct: 244 MKKLHARFDAFLTDILEEHKGKIFGEMKDLLSTLISLKNDDADNDGGKLTDTEIKALLLN 303

Query: 242 IIIGGTDTTSTTIEWTIAELIQQPNIMKKVKEELTKVVGLNQMVEEFHLSKLHYLDAAIK 301
           + + GTDT+S+T+EW IAELI+ P I+ + ++E+ KVVG +++V E  L++L YL+A +K
Sbjct: 304 LFVAGTDTSSSTVEWAIAELIRNPKILAQAQQEIDKVVGRDRLVGELDLAQLTYLEAIVK 363

Query: 302 ETLRLHPPVPLLVPRTTNQRTTLEGYTIPKSSTIYFNIWAIQRDPKIWDNPLDFMPERFL 361
           ET RLHP  PL +PR  ++   + GY IPK ST+  N+WAI RDP  W +PL+F PERFL
Sbjct: 364 ETFRLHPSTPLSLPRIASESCEINGYFIPKGSTLLLNVWAIARDPNAWADPLEFRPERFL 423

Query: 362 NESNENMYDFTGNKIEFCPFGAGKKLCVGIPLAERLLVLILASLLHGFEWKLPEG---ST 421
               +   D  GN  E  PFGAG+++C G+ L  R++ L++A+L+H F W L  G     
Sbjct: 424 PGGEKPKVDVRGNDFEVIPFGAGRRICAGMNLGIRMVQLMIATLIHAFNWDLVSGQLPEM 483

Query: 422 LDLEEKFGIVSKKLNPLVVIPTPRL 440
           L++EE +G+  ++ +PLVV P PRL
Sbjct: 484 LNMEEAYGLTLQRADPLVVHPRPRL 505

BLAST of Cucsa.302220.1 vs. Swiss-Prot
Match: F3PH_ARATH (Flavonoid 3'-monooxygenase OS=Arabidopsis thaliana GN=CYP75B1 PE=1 SV=1)

HSP 1 Score: 295.0 bits (754), Expect = 1.3e-78
Identity = 169/445 (37.98%), Postives = 250/445 (56.18%), Query Frame = 1

Query: 2   YGPIFKLRLGTKLCVVLTSPSSVNEVLRHQETVFDNRDATVSALLATYGGVDIVFSQNES 61
           YGPI  LRLG    VV  S S   + L+  +  F +R     A    Y   D+VF+    
Sbjct: 64  YGPILHLRLGFVDVVVAASKSVAEQFLKIHDANFASRPPNSGAKHMAYNYQDLVFAPYGH 123

Query: 62  IWKKLRKIFARKMLSKSNLDASYPLRRREVRKIIKGVLESAGTPIDISKLSLMAAVKSVM 121
            W+ LRKI +  + S   L+    +R+ EV  + + ++     P+++ +L  M  V ++ 
Sbjct: 124 RWRLLRKISSVHLFSAKALEDFKHVRQEEVGTLTRELVRVGTKPVNLGQLVNMCVVNALG 183

Query: 122 AMTWGGSRGLIGVDGADLEAKFMEVVNELMVLLGTPNLSDLFPVLGGLDLQGIGRKMKKV 181
               G  R L G D      +F  +V E+M L G  N+ D  P L  LDLQG+  KMK++
Sbjct: 184 REMIG--RRLFGADADHKADEFRSMVTEMMALAGVFNIGDFVPSLDWLDLQGVAGKMKRL 243

Query: 182 MNVFDGIFNSAIEEQRKRGGDGMENRGFLQLLLEVMEGED---SSESITDKELKALLVDI 241
              FD   +S ++E    G D  ++   L  L+  ++G D      S+TD E+KALL+++
Sbjct: 244 HKRFDAFLSSILKEHEMNGQD-QKHTDMLSTLIS-LKGTDLDGDGGSLTDTEIKALLLNM 303

Query: 242 IIGGTDTTSTTIEWTIAELIQQPNIMKKVKEELTKVVGLNQMVEEFHLSKLHYLDAAIKE 301
              GTDT+++T++W IAELI+ P+IM K +EEL  VVG ++ V E  +++L YL A IKE
Sbjct: 304 FTAGTDTSASTVDWAIAELIRHPDIMVKAQEELDIVVGRDRPVNESDIAQLPYLQAVIKE 363

Query: 302 TLRLHPPVPLLVPRTTNQRTTLEGYTIPKSSTIYFNIWAIQRDPKIWDNPLDFMPERFLN 361
             RLHPP PL +P   ++   + GY IPK ST+  NIWAI RDP  W +PL F PERFL 
Sbjct: 364 NFRLHPPTPLSLPHIASESCEINGYHIPKGSTLLTNIWAIARDPDQWSDPLAFKPERFLP 423

Query: 362 ESNENMYDFTGNKIEFCPFGAGKKLCVGIPLAERLLVLILASLLHGFEWKLPEGST---L 421
              ++  D  G+  E  PFGAG+++C G+ L  R +  + A+L+ GF+W+L  G T   L
Sbjct: 424 GGEKSGVDVKGSDFELIPFGAGRRICAGLSLGLRTIQFLTATLVQGFDWELAGGVTPEKL 483

Query: 422 DLEEKFGIVSKKLNPLVVIPTPRLS 441
           ++EE +G+  ++  PLVV P PRL+
Sbjct: 484 NMEESYGLTLQRAVPLVVHPKPRLA 504

BLAST of Cucsa.302220.1 vs. Swiss-Prot
Match: C76BA_SWEMU (Geraniol 8-hydroxylase OS=Swertia mussotii GN=CYP76B10 PE=1 SV=1)

HSP 1 Score: 294.3 bits (752), Expect = 2.3e-78
Identity = 167/445 (37.53%), Postives = 260/445 (58.43%), Query Frame = 1

Query: 2   YGPIFKLRLGTKLCVVLTSPSSVNEVLRHQETVFDNRDATVSALLATYGGVDIVFSQNES 61
           +GPI  L+LG    +V+TS     EVL+ Q+  F +R    +          +++    S
Sbjct: 63  HGPIMGLQLGQVTTIVVTSSGMAKEVLQKQDLAFSSRSIPNAIHAHDQYKYSVIWLPVAS 122

Query: 62  IWKKLRKIFARKMLSKSNLDASYPLRRREVRKIIKGVLESA--GTPIDISKLSLMAAVKS 121
            W+ LRK     M S + LDA+  LR R+V+++I    +S+  G  ID+ + +   ++  
Sbjct: 123 RWRGLRKALNSNMFSGNRLDANQHLRSRKVQELIAYCRKSSQTGDAIDVGRAAFRTSLNL 182

Query: 122 VMAMTWGGSRGLIGVDGADLEAKFMEVVNELMVLLGTPNLSDLFPVLGGLDLQGIGRKMK 181
           +    +  S+ L     +D   +F ++V  +MV  G PNL D FP+L  +D QGI ++M 
Sbjct: 183 LSNTMF--SKDLTD-PYSDSAKEFKDLVWNVMVEAGKPNLVDYFPLLDKVDPQGIRKRMT 242

Query: 182 ----KVMNVFDGIFNSAIEEQRKRGGDGMENRGFLQLLLEVMEGEDSSESITDKELKALL 241
               K++ +F G+ +  +++++ +G     N   L +LL     E+S E I    ++ + 
Sbjct: 243 IHFGKILELFGGLIDERLQQKKAKG----VNDDVLDVLLTT--SEESPEEIDRTHIQRMC 302

Query: 242 VDIIIGGTDTTSTTIEWTIAELIQQPNIMKKVKEELTKVVGLNQMVEEFHLSKLHYLDAA 301
           +D+ + GTDTTS+T+EW ++E+++ P  MK  + EL +V+G  + VEE  L++L YL  A
Sbjct: 303 LDLFVAGTDTTSSTLEWAMSEMLKNPEKMKAAQAELAQVIGKGKAVEEADLARLPYLRCA 362

Query: 302 IKETLRLHPPVPLLVPRTTNQRTTLEGYTIPKSSTIYFNIWAIQRDPKIWDNPLDFMPER 361
           IKETLR+HPPVPLL+PR T Q   + GYT+PK+S +  N+WAI RD  IW +PL F PER
Sbjct: 363 IKETLRIHPPVPLLIPRRTEQEVEVCGYTVPKNSQVLVNVWAISRDDAIWKDPLSFKPER 422

Query: 362 FLNESNENMYDFTGNKIEFCPFGAGKKLCVGIPLAERLLVLILASLLHGFEWKLPEG--- 421
           FL    E+  +  G   E  PFGAG+++C G+PLA R++ ++L SLL+ F+WKL  G   
Sbjct: 423 FL----ESELEMRGKDFELIPFGAGRRICPGLPLAVRMVPVMLGSLLNSFDWKLEGGIAP 482

Query: 422 STLDLEEKFGIVSKKLNPLVVIPTP 438
             LD+EEKFGI  +K +PL  + TP
Sbjct: 483 KDLDMEEKFGITLQKAHPLRAVATP 494

BLAST of Cucsa.302220.1 vs. Swiss-Prot
Match: C76C2_ARATH (Cytochrome P450 76C2 OS=Arabidopsis thaliana GN=CYP76C2 PE=2 SV=1)

HSP 1 Score: 287.3 bits (734), Expect = 2.8e-76
Identity = 159/449 (35.41%), Postives = 256/449 (57.02%), Query Frame = 1

Query: 2   YGPIFKLRLGTKLCVVLTSPSSVNEVLRHQETVFDNRDATVSALLATYGGVDIVF-SQNE 61
           YGPI  L+ G+   VV+TSP +  EVLR  + +  +R  T S     +  V +V+   + 
Sbjct: 69  YGPIMSLKFGSLNTVVVTSPEAAREVLRTYDQILSSRTPTNSIRSINHDKVSVVWLPPSS 128

Query: 62  SIWKKLRKIFARKMLSKSNLDASYPLRRREVRKIIKGVLESAGTP--IDISKLSLMAAVK 121
           S W+ LRK+ A ++ S   ++A+  LR  +V++++  + ES+     +DIS+ + + A+ 
Sbjct: 129 SRWRLLRKLSATQLFSPQRIEATKTLRENKVKELVSFMSESSEREEAVDISRATFITALN 188

Query: 122 SVMAMTWGGSRGLIGVDGADLEAKFMEVVNELMVLLGTPNLSDLFPVLGGLDLQGIGRKM 181
            +  + +      +G   ++    F + V  +M  +G P+ ++ FP LG LDLQG  + +
Sbjct: 189 IISNILFSVD---LGNYDSNKSGVFQDTVIGVMEAVGNPDAANFFPFLGFLDLQGNRKTL 248

Query: 182 K----KVMNVFDGIFNSAIEEQ--RKRGGDGMENRGFLQLLLEVMEGEDSSESITDKELK 241
           K    ++  VF G  ++ + E+  R      +  R F+ +LL++ EG+++  +  D  + 
Sbjct: 249 KACSERLFKVFRGFIDAKLAEKSLRDTNSKDVRERDFVDVLLDLTEGDEAELNTND--IV 308

Query: 242 ALLVDIIIGGTDTTSTTIEWTIAELIQQPNIMKKVKEELTKVVGLNQMVEEFHLSKLHYL 301
            LL+D+   GTDT S+T+EW +AEL++ P  M K + E+  V+G   +VEE  +S L YL
Sbjct: 309 HLLLDLFGAGTDTNSSTVEWAMAELLRNPETMVKAQAEIDCVIGQKGVVEESDISALPYL 368

Query: 302 DAAIKETLRLHPPVPLLVPRTTNQRTTLEGYTIPKSSTIYFNIWAIQRDPKIWDNPLDFM 361
            A +KET RLHP  PLLVPR       + G+ +PK + ++ N+WAI RDP +W+N   F 
Sbjct: 369 QAVVKETFRLHPAAPLLVPRKAESDVEVLGFMVPKDTQVFVNVWAIGRDPNVWENSSRFK 428

Query: 362 PERFLNESNENMYDFTGNKIEFCPFGAGKKLCVGIPLAERLLVLILASLLHGFEWKLPEG 421
           PERFL +      D  G   E  PFGAG+++C G+PLA + + L+LASLL+ F+WKLP G
Sbjct: 429 PERFLGKD----IDLRGRDYELTPFGAGRRICPGLPLAVKTVPLMLASLLYSFDWKLPNG 488

Query: 422 ---STLDLEEKFGIVSKKLNPLVVIPTPR 439
                LD++E FG+   K NPL  +P  +
Sbjct: 489 VGSEDLDMDETFGLTLHKTNPLHAVPVKK 508

BLAST of Cucsa.302220.1 vs. Swiss-Prot
Match: C76B6_CATRO (Geraniol 8-hydroxylase OS=Catharanthus roseus GN=CYP76B6 PE=1 SV=1)

HSP 1 Score: 287.0 bits (733), Expect = 3.7e-76
Identity = 159/444 (35.81%), Postives = 257/444 (57.88%), Query Frame = 1

Query: 2   YGPIFKLRLGTKLCVVLTSPSSVNEVLRHQETVFDNRDATVSALLATYGGVDIVFSQNES 61
           +GPI  L+LG    +V++S +   EVL+ Q+  F +R    +          +V+    S
Sbjct: 61  HGPIMSLKLGQITTIVISSSTMAKEVLQKQDLAFSSRSVPNALHAHNQFKFSVVWLPVAS 120

Query: 62  IWKKLRKIFARKMLSKSNLDASYPLRRREVRKIIKGVLESA--GTPIDISKLSLMAAVKS 121
            W+ LRK+    + S + LDA+  LR R+V+++I    +++  G  +D+ + +   ++  
Sbjct: 121 RWRSLRKVLNSNIFSGNRLDANQHLRTRKVQELIAYCRKNSQSGEAVDVGRAAFRTSLNL 180

Query: 122 VMAMTWGGSRGLIGVDGADLEAKFMEVVNELMVLLGTPNLSDLFPVLGGLDLQGIGRKMK 181
           +  + +  S+ L     +D   +F ++V  +MV  G PNL D FP+L  +D QGI  +M 
Sbjct: 181 LSNLIF--SKDLTD-PYSDSAKEFKDLVWNIMVEAGKPNLVDFFPLLEKVDPQGIRHRMT 240

Query: 182 ----KVMNVFDGIFNSAIEEQRKRGGDGMENRGFLQLLLEVMEGEDSSESITDKELKALL 241
               +V+ +F G+ N  +E++R +G    E    L +LL     ++S E I    ++ + 
Sbjct: 241 IHFGEVLKLFGGLVNERLEQRRSKG----EKNDVLDVLLTT--SQESPEEIDRTHIERMC 300

Query: 242 VDIIIGGTDTTSTTIEWTIAELIQQPNIMKKVKEELTKVVGLNQMVEEFHLSKLHYLDAA 301
           +D+ + GTDTTS+T+EW ++E+++ P+ MKK ++EL +V+G  + +EE  +++L YL   
Sbjct: 301 LDLFVAGTDTTSSTLEWAMSEMLKNPDKMKKTQDELAQVIGRGKTIEESDINRLPYLRCV 360

Query: 302 IKETLRLHPPVPLLVPRTTNQRTTLEGYTIPKSSTIYFNIWAIQRDPKIWDNPLDFMPER 361
           +KETLR+HPPVP L+PR   Q   + GY +PK S +  N WAI RD  +WD+ L F PER
Sbjct: 361 MKETLRIHPPVPFLIPRKVEQSVEVCGYNVPKGSQVLVNAWAIGRDETVWDDALAFKPER 420

Query: 362 FLNESNENMYDFTGNKIEFCPFGAGKKLCVGIPLAERLLVLILASLLHGFEWKLPEG--- 421
           F+    E+  D  G   E  PFGAG+++C G+PLA R + L+L SLL+ F WKL  G   
Sbjct: 421 FM----ESELDIRGRDFELIPFGAGRRICPGLPLALRTVPLMLGSLLNSFNWKLEGGMAP 480

Query: 422 STLDLEEKFGIVSKKLNPLVVIPT 437
             LD+EEKFGI  +K +PL  +P+
Sbjct: 481 KDLDMEEKFGITLQKAHPLRAVPS 491

BLAST of Cucsa.302220.1 vs. TrEMBL
Match: M5WDL9_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa019218mg PE=3 SV=1)

HSP 1 Score: 505.4 bits (1300), Expect = 7.3e-140
Identity = 243/452 (53.76%), Postives = 337/452 (74.56%), Query Frame = 1

Query: 1   MYGPIFKLRLGTKLCVVLTSPSSVNEVLRHQETVFDNRDATVSALLATYGGVDIVFSQNE 60
           +YGPI+KL+LG+KLC VL+SPS V +VLR Q+T+F NRD T++A +A+YG  DI F    
Sbjct: 59  IYGPIYKLQLGSKLCFVLSSPSIVKQVLRDQDTLFANRDPTIAAQIASYGCTDIAFGPYG 118

Query: 61  SIWKKLRKIFARKMLSKSNLDASYPLRRREVRKIIKGVLESAGTPIDISKLSLMAAVKSV 120
             W++LRK+F   MLSK+NLDASY LR+ EV K I  V +  G+PID+ + + + ++ +V
Sbjct: 119 PGWRRLRKVFMSHMLSKANLDASYALRKEEVLKSISHVYDKVGSPIDLGQFAFLTSINTV 178

Query: 121 MAMTWGGSRGLIGVDGADLEAKFMEVVNELMVLLGTPNLSDLFPVLGGLDLQGIGRKMKK 180
           M M WGG+  L   +GADL A+F  VV E++ +LG PN+SD    L   D+QGI R+MK+
Sbjct: 179 MRMLWGGT--LEAENGADLGAEFRNVVAEIIEVLGKPNVSDFLTSLARFDVQGIARRMKQ 238

Query: 181 VMNVFDGIFNSAIEEQRKRG---GDGME----NRGFLQLLLEVMEGEDSSESITDKELKA 240
           + ++ + IF+SAIE Q+       +G+E     + FLQ+LLE+ + ED ++SIT ++LKA
Sbjct: 239 LQSMTEKIFDSAIERQKSEAVEKDEGLELKHERKDFLQILLELNDHEDGAKSITLQQLKA 298

Query: 241 LLVDIIIGGTDTTSTTIEWTIAELIQQPNIMKKVKEELTKVVGLNQMVEEFHLSKLHYLD 300
           LL+DI++GGTDTT+T +EW +AELI+  + +KKV+EEL +VVGL  MVEE H+ KLHYLD
Sbjct: 299 LLLDIVVGGTDTTATMVEWVMAELIKHQDDLKKVQEELKEVVGLKNMVEESHIPKLHYLD 358

Query: 301 AAIKETLRLHPPVPLLVPRTTNQRTTLEGYTIPKSSTIYFNIWAIQRDPKIWDNPLDFMP 360
           A IKET RLHP +PLL P   +Q TT+ GY IPK STI+ NIWAI RDP +WDNPL+F P
Sbjct: 359 AVIKETSRLHPALPLLAPHCPSQSTTIGGYNIPKGSTIFINIWAIHRDPNVWDNPLEFRP 418

Query: 361 ERFLNESNENMYDFTGNKIEFCPFGAGKKLCVGIPLAERLLVLILASLLHGFEWKLPEGS 420
           +RFLN+ + N +D+ GNK+E+ PFG+G+++C G+PLAE++++ +LAS LH FEW+LP  +
Sbjct: 419 KRFLNDPSSNNFDYKGNKLEYLPFGSGRRMCAGLPLAEKMMIYVLASFLHSFEWRLPTDA 478

Query: 421 TLDLEEKFGIVSKKLNPLVVIPTPRLSNLELY 446
             DL++KFGIV+KK+ PL+VIPTPRLS LELY
Sbjct: 479 KHDLQDKFGIVTKKMTPLIVIPTPRLSKLELY 508

BLAST of Cucsa.302220.1 vs. TrEMBL
Match: M5WSD8_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa019849mg PE=3 SV=1)

HSP 1 Score: 488.0 bits (1255), Expect = 1.2e-134
Identity = 237/452 (52.43%), Postives = 325/452 (71.90%), Query Frame = 1

Query: 1   MYGPIFKLRLGTKLCVVLTSPSSVNEVLRHQETVFDNRDATVSALLATYGGVDIVFSQNE 60
           +YGPI+KL+LG+KLC VL+SPS V +V+R Q+T+F N D  ++A + +YGG DI F    
Sbjct: 81  VYGPIYKLQLGSKLCFVLSSPSLVKQVVRDQDTLFANHDPIIAAQIISYGGTDIAFGSYG 140

Query: 61  SIWKKLRKIFARKMLSKSNLDASYPLRRREVRKIIKGVLESAGTPIDISKLSLMAAVKSV 120
             W++LRK+F   ++SK NLDA Y LR+ EV K I  +   + +PID  +++   AV ++
Sbjct: 141 PDWRRLRKVFVSHVMSKGNLDACYALRKEEVLKSIDHIYGKSDSPIDFGQVAFFTAVNTI 200

Query: 121 MAMTWGGSRGLIGVDGADLEAKFMEVVNELMVLLGTPNLSDLFPVLGGLDLQGIGRKMKK 180
           M M WGG+  L    G DL A+   VV E++ LLG  N+SD FP L   D+QGI R+ K+
Sbjct: 201 MRMLWGGT--LEAEKGTDLGAELRNVVAEMVELLGKGNISDFFPWLAKFDVQGIARRAKQ 260

Query: 181 VMNVFDGIFNSAIEEQRKRGGD---GM----ENRGFLQLLLEVMEGEDSSESITDKELKA 240
           V++V + I NSAIE+Q     +   G+    E +GFLQ LLE+ E  D +ES+T +++KA
Sbjct: 261 VLSVTEKILNSAIEKQMSEAAEQDGGLSLKHERKGFLQFLLELNEHGDDAESLTLQQIKA 320

Query: 241 LLVDIIIGGTDTTSTTIEWTIAELIQQPNIMKKVKEELTKVVGLNQMVEEFHLSKLHYLD 300
           LL DI+ GGTDTT+T +EW +AEL+QQP+ +KKV+EEL +VVGL  +VEE H+ KLHYLD
Sbjct: 321 LLTDIMAGGTDTTATMMEWVMAELMQQPDDLKKVQEELKEVVGLKNLVEESHIPKLHYLD 380

Query: 301 AAIKETLRLHPPVPLLVPRTTNQRTTLEGYTIPKSSTIYFNIWAIQRDPKIWDNPLDFMP 360
           A IKET RLHP +PLL P   +Q TT+ G+ IPK S+++ N+WAI RDP +WDNPL+F P
Sbjct: 381 AVIKETSRLHPALPLLAPHCPSQSTTIGGFKIPKGSSVFLNVWAIHRDPNVWDNPLEFRP 440

Query: 361 ERFLNESNENMYDFTGNKIEFCPFGAGKKLCVGIPLAERLLVLILASLLHGFEWKLPEGS 420
           +RFLN+   N   + GNK+E+ PFG+G+++C GIPLAER+++ +LAS LH FEW+LP  +
Sbjct: 441 KRFLNDPPTNNIHYNGNKLEYLPFGSGRRMCPGIPLAERMMIYVLASFLHSFEWRLPNDA 500

Query: 421 TLDLEEKFGIVSKKLNPLVVIPTPRLSNLELY 446
            LDL++KFGIV+KK+ PLVVIPTPRLS LELY
Sbjct: 501 KLDLQDKFGIVTKKMTPLVVIPTPRLSKLELY 530

BLAST of Cucsa.302220.1 vs. TrEMBL
Match: A0A067FFE7_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g009481mg PE=3 SV=1)

HSP 1 Score: 486.9 bits (1252), Expect = 2.7e-134
Identity = 242/461 (52.49%), Postives = 326/461 (70.72%), Query Frame = 1

Query: 1   MYGPIFKLRLGTKLCVVLTSPSSVNEVLRHQETVFDNRDATVSALLATYGGVDIVFSQNE 60
           +YGPIFKL LG KLCVV++SPS V +V+R Q+T F +RD  ++ L+AT+GG DI +S   
Sbjct: 73  VYGPIFKLWLGNKLCVVVSSPSLVKQVVRDQDTTFADRDPPIAGLVATFGGNDIAWSNYG 132

Query: 61  SIWKKLRKIFARKMLSKSNLDASYPLRRREVRKIIKGVLES---AGTPIDISKLSLMAAV 120
             W KLRK F  KM+S ++LDA Y LR++EV+  I+ +  +    G PIDI +LS+   V
Sbjct: 133 PEWSKLRKFFVGKMMSNASLDACYALRKQEVKNTIRDLYNNDNKIGKPIDIGELSISTLV 192

Query: 121 KSVMAMTWGGSRGLIGVDGADLEAKFMEVVNELMVLLGTPNLSDLFPVLGGLDLQGIGRK 180
             +  + WG +  +      +L A+    + ELMVL+GTPN+SD+FPVL   D+QGI RK
Sbjct: 193 CVIQNILWGEALEIREEGITNLGAELKFKLAELMVLMGTPNISDVFPVLSWFDIQGIERK 252

Query: 181 MKKVMNVFDGIFNSAIEEQR---------KRGG---DGMENRGFLQLLLEVMEGEDSSES 240
            KK+   F+ I NS +E+ R         +R G   +G  N+ FLQLLLE+ E ED S S
Sbjct: 253 AKKISLWFENIINSTVEKYRSKDFIVEGKERAGKKFEGSRNKNFLQLLLELQENEDGSSS 312

Query: 241 ITDKELKALLVDIIIGGTDTTSTTIEWTIAELIQQPNIMKKVKEELTKVVGLNQMVEEFH 300
           I+  + K +LVDII GGTDTT+T +EWT+AEL+Q P +MKKV+EEL +VVG++  VEEFH
Sbjct: 313 ISMNKFKGVLVDIITGGTDTTTTMVEWTMAELMQHPQVMKKVQEELAQVVGMDSCVEEFH 372

Query: 301 LSKLHYLDAAIKETLRLHPPVPLLVPRTTNQRTTLEGYTIPKSSTIYFNIWAIQRDPKIW 360
           L KL YLDA +KET RLHP +PLLVPR  ++  ++ GYTIPK +T+  N+WAI RDP++W
Sbjct: 373 LPKLKYLDAVVKETFRLHPALPLLVPRRASESGSIGGYTIPKDTTLMLNVWAIHRDPQLW 432

Query: 361 DNPLDFMPERFLNESNENMYDFTGNKIEFCPFGAGKKLCVGIPLAERLLVLILASLLHGF 420
           DNPL+F PERFLN   E+ +D++GN  ++ PFG+G+++C GIPLAER+L+ +LASLLH F
Sbjct: 433 DNPLEFRPERFLNVGVESKFDYSGNNFQYLPFGSGRRMCAGIPLAERMLMFVLASLLHSF 492

Query: 421 EWKLPEGSTLDLEEKFGIVSKKLNPLVVIPTPRLSNLELYN 447
           EW+LP G+ L+L E FGIV KK  PL+ IPTPRLSN ELY+
Sbjct: 493 EWELPAGTKLNLSENFGIVIKKKEPLIAIPTPRLSNSELYH 533

BLAST of Cucsa.302220.1 vs. TrEMBL
Match: V4U067_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10014858mg PE=3 SV=1)

HSP 1 Score: 484.6 bits (1246), Expect = 1.3e-133
Identity = 245/463 (52.92%), Postives = 328/463 (70.84%), Query Frame = 1

Query: 1   MYGPIFKLRLGTKLCVVLTSPSSVNEVLRHQETVFDNRDATVSALLATYGGVDIVFSQNE 60
           +YGPIFKL LG KLCVV++SPS V +V+R Q+T F  RD  ++ L+AT+GG DI +S   
Sbjct: 73  VYGPIFKLWLGNKLCVVVSSPSLVKQVVRDQDTTFAYRDPPIAGLVATFGGNDIAWSNYG 132

Query: 61  SIWKKLRKIFARKMLSKSNLDASYPLRRREVRKIIKGVLESAGT---PIDISKLSLMAAV 120
             W+KLRK F  KM+S ++LDA Y LR++EV+  I+ +  +      PIDI +LS+   V
Sbjct: 133 PEWRKLRKFFVGKMMSNASLDACYALRKQEVKNTIRDLYNNNNKICKPIDIGELSISTLV 192

Query: 121 KSVMAMTWGGSRGLI--GVDGADLEAKFMEVVNELMVLLGTPNLSDLFPVLGGLDLQGIG 180
            ++  M WG +  L   G     +E KF   + ELMVLLGTPN+SD+FP+L  LD+QGI 
Sbjct: 193 CAMQNMLWGEALELKEKGTTILGVELKFK--LAELMVLLGTPNISDIFPMLSWLDIQGIE 252

Query: 181 RKMKKVMNVFDGIFNSAIEEQR---------KRGGDGME---NRGFLQLLLEVMEGEDSS 240
           R+ KK+   F+ I NS +E+ R         +R G+  E   N+ FLQLLLE+ E ED S
Sbjct: 253 RRAKKISLWFENIINSTVEKYRSKDFNVEGKERAGENFEGSRNKNFLQLLLELQENEDGS 312

Query: 241 ESITDKELKALLVDIIIGGTDTTSTTIEWTIAELIQQPNIMKKVKEELTKVVGLNQMVEE 300
            SI+  + K +L DII GGTDTT+T +EWT+AEL+Q P +MKKV+EEL +VVG++  VEE
Sbjct: 313 SSISMNQFKGVLADIITGGTDTTTTMVEWTMAELMQHPQVMKKVQEELAQVVGMDSCVEE 372

Query: 301 FHLSKLHYLDAAIKETLRLHPPVPLLVPRTTNQRTTLEGYTIPKSSTIYFNIWAIQRDPK 360
           FHL KL YLDA +KE+ RLHP +PLLVPR  ++  ++ GYTIPK +T+  N+WAI RDP+
Sbjct: 373 FHLPKLKYLDAVVKESFRLHPALPLLVPRRASESGSIGGYTIPKDTTLMLNVWAIHRDPQ 432

Query: 361 IWDNPLDFMPERFLNESNENMYDFTGNKIEFCPFGAGKKLCVGIPLAERLLVLILASLLH 420
           +WDNPL+F PERFLN   E+ +D++GN  ++ PFG+G+++C GIPLAER+L+ +LASLLH
Sbjct: 433 LWDNPLEFRPERFLNVGVESKFDYSGNNFQYLPFGSGRRMCAGIPLAERMLMFVLASLLH 492

Query: 421 GFEWKLPEGSTLDLEEKFGIVSKKLNPLVVIPTPRLSNLELYN 447
            FEW+LP G+ L+L EKFGIV KK  PLV IPTPRLSN ELY+
Sbjct: 493 SFEWELPAGTKLNLSEKFGIVIKKKEPLVAIPTPRLSNSELYH 533

BLAST of Cucsa.302220.1 vs. TrEMBL
Match: A0A067FBZ9_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g009495mg PE=3 SV=1)

HSP 1 Score: 484.6 bits (1246), Expect = 1.3e-133
Identity = 245/463 (52.92%), Postives = 328/463 (70.84%), Query Frame = 1

Query: 1   MYGPIFKLRLGTKLCVVLTSPSSVNEVLRHQETVFDNRDATVSALLATYGGVDIVFSQNE 60
           +YGPIFKL LG KLCVV++SPS V +V+R Q+T F  RD  ++ L+AT+GG DI +S   
Sbjct: 73  VYGPIFKLWLGNKLCVVVSSPSLVKQVVRDQDTTFAYRDPPIAGLVATFGGNDIAWSNYG 132

Query: 61  SIWKKLRKIFARKMLSKSNLDASYPLRRREVRKIIKGVLESAGT---PIDISKLSLMAAV 120
             W+KLRK F  KM+S ++LDA Y LR++EV+  I+ +  +      PIDI +LS+   V
Sbjct: 133 PEWRKLRKFFVGKMMSNASLDACYALRKQEVKNTIRDLYNNNNKICKPIDIGELSISTLV 192

Query: 121 KSVMAMTWGGSRGLI--GVDGADLEAKFMEVVNELMVLLGTPNLSDLFPVLGGLDLQGIG 180
            ++  M WG +  L   G     +E KF   + ELMVLLGTPN+SD+FP+L  LD+QGI 
Sbjct: 193 CAMQNMLWGEALELKEKGTTILGVELKFK--LAELMVLLGTPNISDIFPMLSWLDIQGIE 252

Query: 181 RKMKKVMNVFDGIFNSAIEEQR---------KRGGDGME---NRGFLQLLLEVMEGEDSS 240
           R+ KK+   F+ I NS +E+ R         +R G+  E   N+ FLQLLLE+ E ED S
Sbjct: 253 RRAKKISLWFENIINSTVEKYRSKDFNVEGKERAGENFEGSRNKNFLQLLLELQENEDGS 312

Query: 241 ESITDKELKALLVDIIIGGTDTTSTTIEWTIAELIQQPNIMKKVKEELTKVVGLNQMVEE 300
            SI+  + K +L DII GGTDTT+T +EWT+AEL+Q P +MKKV+EEL +VVG++  VEE
Sbjct: 313 SSISMNQFKGVLADIITGGTDTTTTMVEWTMAELMQHPQVMKKVQEELAQVVGMDSCVEE 372

Query: 301 FHLSKLHYLDAAIKETLRLHPPVPLLVPRTTNQRTTLEGYTIPKSSTIYFNIWAIQRDPK 360
           FHL KL YLDA +KE+ RLHP +PLLVPR  ++  ++ GYTIPK +T+  N+WAI RDP+
Sbjct: 373 FHLPKLKYLDAVVKESFRLHPALPLLVPRRASESGSIGGYTIPKDTTLMLNVWAIHRDPQ 432

Query: 361 IWDNPLDFMPERFLNESNENMYDFTGNKIEFCPFGAGKKLCVGIPLAERLLVLILASLLH 420
           +WDNPL+F PERFLN   E+ +D++GN  ++ PFG+G+++C GIPLAER+L+ +LASLLH
Sbjct: 433 LWDNPLEFRPERFLNVGVESKFDYSGNNFQYLPFGSGRRMCAGIPLAERMLMFVLASLLH 492

Query: 421 GFEWKLPEGSTLDLEEKFGIVSKKLNPLVVIPTPRLSNLELYN 447
            FEW+LP G+ L+L EKFGIV KK  PLV IPTPRLSN ELY+
Sbjct: 493 SFEWELPAGTKLNLSEKFGIVIKKKEPLVAIPTPRLSNSELYH 533

BLAST of Cucsa.302220.1 vs. TAIR10
Match: AT4G12320.1 (AT4G12320.1 cytochrome P450, family 706, subfamily A, polypeptide 6)

HSP 1 Score: 397.5 bits (1020), Expect = 1.1e-110
Identity = 201/453 (44.37%), Postives = 303/453 (66.89%), Query Frame = 1

Query: 2   YGPIFKLRLGTKLCVVLTSPSSVNEVLRHQETVFDNRDATVSALLATYGGVDIVFSQNES 61
           YGPIFKL LG+KL VV+ +PS   E+L+ Q+  F N D  ++A   TYGG+D+V+    +
Sbjct: 73  YGPIFKLNLGSKLTVVVNTPSLAREILKDQDINFSNHDVPLTARAVTYGGLDLVWLPYGA 132

Query: 62  IWKKLRKIFARKMLSKSNLDASYPLRRREVRKIIKGVLESAG--TPIDISKLSLMAAVKS 121
            W+ LRK+   K+LS   L++ Y LRR+E+R+  + + +     +P+++ +   +  +  
Sbjct: 133 EWRMLRKVCVLKLLSHRTLNSFYELRRKEIRERTRYLYQKGQEESPVNVGEQVFLTMMNL 192

Query: 122 VMAMTWGGSRGLIGVDGADLEA---KFMEVVNELMVLLGTPNLSDLFPVLGGLDLQGIGR 181
            M M WGGS     V   ++E+   +F EV++E+  LLG PN+SD FP L   DLQG+ +
Sbjct: 193 TMNMLWGGS-----VKAEEMESVGTEFKEVISEITRLLGEPNVSDFFPRLARFDLQGLVK 252

Query: 182 KMKKVMNVFDGIFNSAIEEQ---RKRGGDGMENRGFLQLLLEVMEGE-DSSESITDKELK 241
           KM       D I + AIE+    R R GD  E + FLQ L+++ + E DS   IT   +K
Sbjct: 253 KMHVCARELDAILDRAIEQMQLLRTRDGDDGECKDFLQHLMKLKDQEADSEVPITVNHVK 312

Query: 242 ALLVDIIIGGTDTTSTTIEWTIAELIQQPNIMKKVKEELTKVVGLNQMVEEFHLSKLHYL 301
           A+LVD+++GGTDT++ TIE+ +AELI++P +MK+ ++EL +VVG + ++EE H+++L ++
Sbjct: 313 AVLVDLVVGGTDTSTNTIEFAMAELIRKPELMKRAQQELDEVVGKDNIIEESHITRLPFI 372

Query: 302 DAAIKETLRLHPPVPLLVPRTTNQRTTLEGYTIPKSSTIYFNIWAIQRDPKIWDNPLDFM 361
            A +KETLRL+P +PLLVP   ++   + GYTIPK++ I+ N+W+IQRDP +W+ P +F 
Sbjct: 373 SAIMKETLRLYPTIPLLVPHRPSETALVGGYTIPKNTKIFINVWSIQRDPNVWEYPTEFR 432

Query: 362 PERFLNESNENMYDFTGNKIEFCPFGAGKKLCVGIPLAERLLVLILASLLHGFEWKLPEG 421
           PERFL++ +    DFTG    + PFG+G+++C GI LAER+++  LA+LLH F+WK+PEG
Sbjct: 433 PERFLDKKS---CDFTGTDYSYLPFGSGRRICAGIALAERMILYTLATLLHSFDWKIPEG 492

Query: 422 STLDLEEKFGIVSKKLNPLVVIPTPRLSNLELY 446
             LDL+EKFGIV K  +PLV +P PRLSN  LY
Sbjct: 493 HILDLKEKFGIVLKLKSPLVALPVPRLSNSNLY 517

BLAST of Cucsa.302220.1 vs. TAIR10
Match: AT4G12300.1 (AT4G12300.1 cytochrome P450, family 706, subfamily A, polypeptide 4)

HSP 1 Score: 396.0 bits (1016), Expect = 3.2e-110
Identity = 203/451 (45.01%), Postives = 297/451 (65.85%), Query Frame = 1

Query: 2   YGPIFKLRLGTKLCVVLTSPSSVNEVLRHQETVFDNRDATVSALLATYGGVDIVFSQNES 61
           +GPIFKL LG+KL +V+ SPS   E+L+ Q+  F NRD  ++   ATYGG+DIV++   +
Sbjct: 73  HGPIFKLNLGSKLTIVVNSPSLAREILKDQDINFSNRDVPLTGRAATYGGIDIVWTPYGA 132

Query: 62  IWKKLRKIFARKMLSKSNLDASYPLRRREVRKIIKGVLESAG--TPIDISKLSLMAAVKS 121
            W++LRKI   K+LS+  LD+ Y LRR+EVR+  + + E     +P+ +     +  +  
Sbjct: 133 EWRQLRKICVLKLLSRKTLDSFYELRRKEVRERTRYLYEQGRKQSPVKVGDQLFLTMMNL 192

Query: 122 VMAMTWGGSRGLIGVDGADLEA---KFMEVVNELMVLLGTPNLSDLFPVLGGLDLQGIGR 181
            M M WGGS     V   ++E+   +F  V++E+  LL  PN+SD FP L   DLQG+ +
Sbjct: 193 TMNMLWGGS-----VKAEEMESVGTEFKGVISEITRLLSEPNVSDFFPWLARFDLQGLVK 252

Query: 182 KMKKVMNVFDGIFNSAIEEQRK-RGGDGMENRGFLQLLLEVMEGEDSSE-SITDKELKAL 241
           +M       D + + AIE+ +  RG D  E + FLQ L+++ + E  SE  IT   +KAL
Sbjct: 253 RMGVCARELDAVLDRAIEQMKPLRGRDDDEVKDFLQYLMKLKDQEGDSEVPITINHVKAL 312

Query: 242 LVDIIIGGTDTTSTTIEWTIAELIQQPNIMKKVKEELTKVVGLNQMVEEFHLSKLHYLDA 301
           L D+++GGTDT++ TIE+ +AEL+  P ++K+ +EEL +VVG + +VEE H+++L Y+ A
Sbjct: 313 LTDMVVGGTDTSTNTIEFAMAELMSNPELIKRAQEELDEVVGKDNIVEESHITRLPYILA 372

Query: 302 AIKETLRLHPPVPLLVPRTTNQRTTLEGYTIPKSSTIYFNIWAIQRDPKIWDNPLDFMPE 361
            +KETLRLHP +PLLVP    + T + GYTIPK + I+ N+W+IQRDP +W+NP +F PE
Sbjct: 373 IMKETLRLHPTLPLLVPHRPAENTVVGGYTIPKDTKIFVNVWSIQRDPNVWENPTEFRPE 432

Query: 362 RFLNESNENMYDFTGNKIEFCPFGAGKKLCVGIPLAERLLVLILASLLHGFEWKLPEGST 421
           RFL   + N  DFTG    + PFG+G+++C G+ LAER+++  LA+LLH F+WK+PEG  
Sbjct: 433 RFL---DNNSCDFTGANYSYFPFGSGRRICAGVALAERMVLYTLATLLHSFDWKIPEGHV 492

Query: 422 LDLEEKFGIVSKKLNPLVVIPTPRLSNLELY 446
           LDL+EKFGIV K   PLV +P PR S+  LY
Sbjct: 493 LDLKEKFGIVLKLKIPLVALPIPRFSDSNLY 515

BLAST of Cucsa.302220.1 vs. TAIR10
Match: AT4G12310.1 (AT4G12310.1 cytochrome P450, family 706, subfamily A, polypeptide 5)

HSP 1 Score: 392.1 bits (1006), Expect = 4.6e-109
Identity = 198/453 (43.71%), Postives = 300/453 (66.23%), Query Frame = 1

Query: 2   YGPIFKLRLGTKLCVVLTSPSSVNEVLRHQETVFDNRDATVSALLATYGGVDIVFSQNES 61
           +GPIFKL LG+KL VV+ SPS  +E+L+ Q+  F N D  ++A   TYGG+D+V+    +
Sbjct: 75  HGPIFKLNLGSKLTVVVNSPSLASEILKDQDINFSNHDVPLTARAVTYGGLDLVWLPYGA 134

Query: 62  IWKKLRKIFARKMLSKSNLDASYPLRRREVRKIIKGVLESA--GTPIDISKLSLMAAVKS 121
            W+ LRK+ A K+ S+  LD+ Y LRR+E+R+  + + +     +P+++ +   +  +  
Sbjct: 135 EWRMLRKVCAAKLFSRKTLDSFYELRRKEIRERTRCLYQKGLEKSPVNVGEQLFLTMMNL 194

Query: 122 VMAMTWGGSRGLIGVDGADLEA---KFMEVVNELMVLLGTPNLSDLFPVLGGLDLQGIGR 181
           +M M WGGS     V   D+E+   +F  V++E+  LLG PN+SD FP+L   DLQG+ +
Sbjct: 195 MMNMLWGGS-----VKAEDMESVGTEFKGVISEITRLLGVPNVSDFFPMLARFDLQGLVK 254

Query: 182 KMKKVMNVFDGIFNSAIEEQ---RKRGGDGMENRGFLQLLLEVMEGE-DSSESITDKELK 241
           KM       D I + AIE+    R R GD  E + FLQ L+++ + E DS   IT   +K
Sbjct: 255 KMHLYARDLDAILDRAIEQMQRLRSRDGDDGECKDFLQHLMKLRDQEADSDVPITMNHVK 314

Query: 242 ALLVDIIIGGTDTTSTTIEWTIAELIQQPNIMKKVKEELTKVVGLNQMVEEFHLSKLHYL 301
           A+L+D+++GGT++++ TIE+ +AELI  P +M++ ++EL +VVG + +VEE H++ L Y+
Sbjct: 315 AVLMDMVVGGTESSTNTIEFVMAELISNPELMRRAQQELDEVVGKDNIVEESHITSLPYI 374

Query: 302 DAAIKETLRLHPPVPLLVPRTTNQRTTLEGYTIPKSSTIYFNIWAIQRDPKIWDNPLDFM 361
            A +KETLRL+P +PLLVP   ++   + GYTIPK++ I+ N+W+IQRDP +W+ P +F 
Sbjct: 375 LAVLKETLRLYPTIPLLVPHRPSETALVGGYTIPKNTKIFINVWSIQRDPNVWEYPTEFR 434

Query: 362 PERFLNESNENMYDFTGNKIEFCPFGAGKKLCVGIPLAERLLVLILASLLHGFEWKLPEG 421
           PERFL++ +    DFTG    + PFG+G+++C GI LAER+++  LA+LLH F+W +P+G
Sbjct: 435 PERFLDKKS---CDFTGTDYSYLPFGSGRRICAGIALAERMILYTLATLLHSFDWTIPDG 494

Query: 422 STLDLEEKFGIVSKKLNPLVVIPTPRLSNLELY 446
             LDLEEKFGIV K   PLV +P PRLSN   Y
Sbjct: 495 HVLDLEEKFGIVLKLKTPLVALPIPRLSNSNFY 519

BLAST of Cucsa.302220.1 vs. TAIR10
Match: AT5G44620.1 (AT5G44620.1 cytochrome P450, family 706, subfamily A, polypeptide 3)

HSP 1 Score: 369.0 bits (946), Expect = 4.1e-102
Identity = 189/453 (41.72%), Postives = 289/453 (63.80%), Query Frame = 1

Query: 2   YGPIFKLRLGTKLCVVLTSPSSVNEVLRHQETVFDNRDATVSALLATYGGVDIVFSQNES 61
           +GPIFKL LG KL +V+TS     E+L+  + +F N D      + TYGG +I++S    
Sbjct: 77  HGPIFKLWLGAKLTIVVTSSEVAQEILKTNDIIFANHDVPAVGPVNTYGGTEIIWSPYGP 136

Query: 62  IWKKLRKIFARKMLSKSNLDASYPLRRREVRKIIKGVLESA--GTPIDISKLSLMAAVKS 121
            W+ LRK+   ++L  + LD+S  LRRRE R+ ++ + + A  G+P+++ +   +  +  
Sbjct: 137 KWRMLRKLCVNRILRNAMLDSSTDLRRRETRQTVRYLADQARVGSPVNLGEQIFLMMLNV 196

Query: 122 VMAMTWGGS-----RGLIGVDGADLEAKFMEVVNELMVLLGTPNLSDLFPVLGGLDLQGI 181
           V  M WG +     R ++G       A+F+EV+ E+  LL  PN+SD FPVL   DLQG+
Sbjct: 197 VTQMLWGTTVKEEEREVVG-------AEFLEVIREMNDLLLVPNISDFFPVLSRFDLQGL 256

Query: 182 GRKMKKVMNVFDGIFNSAIEEQRKRGGDGMENRG--FLQLLLEVMEGEDSSESITDKELK 241
            ++M++     D +F+  I ++     D  + R   FL +LL+V + E     +T  ++K
Sbjct: 257 AKRMRRPAQRMDQMFDRIINQRLGMDRDSSDGRAVDFLDVLLKVKDEEAEKTKLTMNDVK 316

Query: 242 ALLVDIIIGGTDTTSTTIEWTIAELIQQPNIMKKVKEELTKVVGLNQMVEEFHLSKLHYL 301
           A+L+D+++GGTDT+   IE+ +AEL+  P+IMK+ ++E+ KVVG  ++VEE H+SKL Y+
Sbjct: 317 AVLMDMVLGGTDTSLHVIEFAMAELLHNPDIMKRAQQEVDKVVGKEKVVEESHISKLPYI 376

Query: 302 DAAIKETLRLHPPVPLLVPRTTNQRTTLEGYTIPKSSTIYFNIWAIQRDPKIWDNPLDFM 361
            A +KETLRLH   PLLVPR  +Q T + G+TIPK S I+ N WAI R+P +W+NPL F 
Sbjct: 377 LAIMKETLRLHTVAPLLVPRRPSQTTVVGGFTIPKDSKIFINAWAIHRNPNVWENPLKFD 436

Query: 362 PERFLNESNENMYDFTGNKIEFCPFGAGKKLCVGIPLAERLLVLILASLLHGFEWKLPEG 421
           P+RFL+ S    YDF GN   + PFG+G+++CVG+ + ER+++  LA+ LH F+WK+P+G
Sbjct: 437 PDRFLDMS----YDFKGNDFNYLPFGSGRRICVGMAMGERVVLYNLATFLHSFDWKIPQG 496

Query: 422 STLDLEEKFGIVSKKLNPLVVIPTPRLSNLELY 446
             +++EEKFGIV +  NPLV  P  RLS+  LY
Sbjct: 497 ERVEVEEKFGIVLELKNPLVATPVLRLSDPNLY 518

BLAST of Cucsa.302220.1 vs. TAIR10
Match: AT4G12330.1 (AT4G12330.1 cytochrome P450, family 706, subfamily A, polypeptide 7)

HSP 1 Score: 366.7 bits (940), Expect = 2.0e-101
Identity = 185/451 (41.02%), Postives = 294/451 (65.19%), Query Frame = 1

Query: 2   YGPIFKLRLGTKLCVVLTSPSSVNEVLRHQETVFDNRDATVSALLATYGGVDIVFSQNES 61
           +GP+FKL LG KL +V+TS  +  ++LR  + +F N D  V+  L+TYGGVDIV+S    
Sbjct: 77  HGPVFKLWLGAKLTIVITSSEATRDILRTNDVIFANDDVPVAGSLSTYGGVDIVWSPYGP 136

Query: 62  IWKKLRKIFARKMLSKSNLDASY--PLRRREVRKIIKGVLESA--GTPIDISKLSLMAAV 121
            W  LRKI   KMLS + LD++    LRR+E R+ ++ + + A  G  +++ +   +  +
Sbjct: 137 EWPMLRKICINKMLSNATLDSNSFSALRRQETRRTVRYLADRARAGLAVNVGEQIFVTIL 196

Query: 122 KSVMAMTWGGSRGLIGVDGADLE---AKFMEVVNELMVLLGTPNLSDLFPVLGGLDLQGI 181
             V  M WG +      D  + E   A+F+E++ E++ ++G PN+SD FPVL   DLQG+
Sbjct: 197 NVVTQMLWGETVA----DDEEREKVGAEFLELITEIIDVVGKPNVSDFFPVLSRFDLQGL 256

Query: 182 GRKMKKVMNVFDGIFNSAIEEQRKRGGDGMENRGFLQLLLEVMEGEDSSESITDKELKAL 241
            +++++     D +F+  I ++         N G    L+ ++  +D  E+++   +KAL
Sbjct: 257 AKRVRRSAQRMDRMFDRIISQRMGMDKGSKGNGG--DFLMVLLNAKDEDENMSMNHVKAL 316

Query: 242 LVDIIIGGTDTTSTTIEWTIAELIQQPNIMKKVKEELTKVVGLNQMVEEFHLSKLHYLDA 301
           L+D+++GGTDT+  TIE+ +AELI +  IMK+ ++EL KVVG N +VEE H++KL Y+ +
Sbjct: 317 LMDMVLGGTDTSLNTIEFAMAELINKLEIMKRAQQELDKVVGKNNIVEEKHITKLPYILS 376

Query: 302 AIKETLRLHPPVPLLVPRTTNQRTTLEGYTIPKSSTIYFNIWAIQRDPKIWDNPLDFMPE 361
            +KETLRLHP +PLL+PR  ++ T + GYTIP  S ++ N+WAI R+P +W+NPL+F P+
Sbjct: 377 IMKETLRLHPALPLLIPRCPSETTVIGGYTIPNDSKVFINVWAIHRNPNVWENPLEFNPD 436

Query: 362 RFLNESNENMYDFTGNKIEFCPFGAGKKLCVGIPLAERLLVLILASLLHGFEWKLPEGST 421
           RFL++     YDF+GN   + PFG+G+++C G+ +AE++++  LA+LLH F+W++ EG  
Sbjct: 437 RFLDKG----YDFSGNDYSYFPFGSGRRICAGMAMAEKVVLYNLATLLHSFDWRIGEGEK 496

Query: 422 LDLEEKFGIVSKKLNPLVVIPTPRLSNLELY 446
           ++LEEKFGI+ K  NPLV  P  RLS+  L+
Sbjct: 497 VELEEKFGILLKLKNPLVATPVLRLSDPNLF 517

BLAST of Cucsa.302220.1 vs. NCBI nr
Match: gi|778699919|ref|XP_004147610.2| (PREDICTED: geraniol 8-hydroxylase-like [Cucumis sativus])

HSP 1 Score: 884.0 bits (2283), Expect = 1.1e-253
Identity = 448/448 (100.00%), Postives = 448/448 (100.00%), Query Frame = 1

Query: 1   MYGPIFKLRLGTKLCVVLTSPSSVNEVLRHQETVFDNRDATVSALLATYGGVDIVFSQNE 60
           MYGPIFKLRLGTKLCVVLTSPSSVNEVLRHQETVFDNRDATVSALLATYGGVDIVFSQNE
Sbjct: 79  MYGPIFKLRLGTKLCVVLTSPSSVNEVLRHQETVFDNRDATVSALLATYGGVDIVFSQNE 138

Query: 61  SIWKKLRKIFARKMLSKSNLDASYPLRRREVRKIIKGVLESAGTPIDISKLSLMAAVKSV 120
           SIWKKLRKIFARKMLSKSNLDASYPLRRREVRKIIKGVLESAGTPIDISKLSLMAAVKSV
Sbjct: 139 SIWKKLRKIFARKMLSKSNLDASYPLRRREVRKIIKGVLESAGTPIDISKLSLMAAVKSV 198

Query: 121 MAMTWGGSRGLIGVDGADLEAKFMEVVNELMVLLGTPNLSDLFPVLGGLDLQGIGRKMKK 180
           MAMTWGGSRGLIGVDGADLEAKFMEVVNELMVLLGTPNLSDLFPVLGGLDLQGIGRKMKK
Sbjct: 199 MAMTWGGSRGLIGVDGADLEAKFMEVVNELMVLLGTPNLSDLFPVLGGLDLQGIGRKMKK 258

Query: 181 VMNVFDGIFNSAIEEQRKRGGDGMENRGFLQLLLEVMEGEDSSESITDKELKALLVDIII 240
           VMNVFDGIFNSAIEEQRKRGGDGMENRGFLQLLLEVMEGEDSSESITDKELKALLVDIII
Sbjct: 259 VMNVFDGIFNSAIEEQRKRGGDGMENRGFLQLLLEVMEGEDSSESITDKELKALLVDIII 318

Query: 241 GGTDTTSTTIEWTIAELIQQPNIMKKVKEELTKVVGLNQMVEEFHLSKLHYLDAAIKETL 300
           GGTDTTSTTIEWTIAELIQQPNIMKKVKEELTKVVGLNQMVEEFHLSKLHYLDAAIKETL
Sbjct: 319 GGTDTTSTTIEWTIAELIQQPNIMKKVKEELTKVVGLNQMVEEFHLSKLHYLDAAIKETL 378

Query: 301 RLHPPVPLLVPRTTNQRTTLEGYTIPKSSTIYFNIWAIQRDPKIWDNPLDFMPERFLNES 360
           RLHPPVPLLVPRTTNQRTTLEGYTIPKSSTIYFNIWAIQRDPKIWDNPLDFMPERFLNES
Sbjct: 379 RLHPPVPLLVPRTTNQRTTLEGYTIPKSSTIYFNIWAIQRDPKIWDNPLDFMPERFLNES 438

Query: 361 NENMYDFTGNKIEFCPFGAGKKLCVGIPLAERLLVLILASLLHGFEWKLPEGSTLDLEEK 420
           NENMYDFTGNKIEFCPFGAGKKLCVGIPLAERLLVLILASLLHGFEWKLPEGSTLDLEEK
Sbjct: 439 NENMYDFTGNKIEFCPFGAGKKLCVGIPLAERLLVLILASLLHGFEWKLPEGSTLDLEEK 498

Query: 421 FGIVSKKLNPLVVIPTPRLSNLELYNMM 449
           FGIVSKKLNPLVVIPTPRLSNLELYNMM
Sbjct: 499 FGIVSKKLNPLVVIPTPRLSNLELYNMM 526

BLAST of Cucsa.302220.1 vs. NCBI nr
Match: gi|659073586|ref|XP_008437142.1| (PREDICTED: flavonoid 3'-monooxygenase-like [Cucumis melo])

HSP 1 Score: 752.3 bits (1941), Expect = 4.9e-214
Identity = 376/448 (83.93%), Postives = 404/448 (90.18%), Query Frame = 1

Query: 1   MYGPIFKLRLGTKLCVVLTSPSSVNEVLRHQETVFDNRDATVSALLATYGGVDIVFSQNE 60
           +YGPIFKLRLG KLC VLTSPSS+NEVLRH ETVFDNRDATVSA L TYGG DIVFSQNE
Sbjct: 74  IYGPIFKLRLGAKLCFVLTSPSSINEVLRHHETVFDNRDATVSARLCTYGGSDIVFSQNE 133

Query: 61  SIWKKLRKIFARKMLSKSNLDASYPLRRREVRKIIKGVLESAGTPIDISKLSLMAAVKSV 120
             WKKLRKIFARKMLSK+NLDASYPLRR+EVRKIIKGVLESAGTPID  +LS +AAVKSV
Sbjct: 134 GDWKKLRKIFARKMLSKANLDASYPLRRQEVRKIIKGVLESAGTPIDFGELSFLAAVKSV 193

Query: 121 MAMTWGGSRGLIGVDGADLEAKFMEVVNELMVLLGTPNLSDLFPVLGGLDLQGIGRKMKK 180
           MAM WGGS GL GV   DLEAKF EVV+ELMVLLGTPNLSDLFPVLG LDLQGI RKMKK
Sbjct: 194 MAMIWGGSGGLTGVAVTDLEAKFREVVDELMVLLGTPNLSDLFPVLGCLDLQGIARKMKK 253

Query: 181 VMNVFDGIFNSAIEEQRKRGGDGMENRGFLQLLLEVMEGEDSSESITDKELKALLVDIII 240
           V N  D I NSAIE QRK+G +G+ENRGFL+ LLEVMEGED+SESIT+ +LK LL+DI+I
Sbjct: 254 VSNRCDEILNSAIEGQRKKGRNGLENRGFLEFLLEVMEGEDNSESITENQLKGLLIDIVI 313

Query: 241 GGTDTTSTTIEWTIAELIQQPNIMKKVKEELTKVVGLNQMVEEFHLSKLHYLDAAIKETL 300
           GGTDTTST IEWT+ EL+QQPN MKKV EELT VVGLNQMVEEFHLSKLHYLDAAIKETL
Sbjct: 314 GGTDTTSTVIEWTMTELLQQPNTMKKVIEELTNVVGLNQMVEEFHLSKLHYLDAAIKETL 373

Query: 301 RLHPPVPLLVPRTTNQRTTLEGYTIPKSSTIYFNIWAIQRDPKIWDNPLDFMPERFLNES 360
           RLHPPV LLVPR +++ TTLEGYTIPK STIYFN+WAIQRDPK+W+NPLDFMPERFLNES
Sbjct: 374 RLHPPVSLLVPRVSSKTTTLEGYTIPKGSTIYFNVWAIQRDPKVWENPLDFMPERFLNES 433

Query: 361 NENMYDFTGNKIEFCPFGAGKKLCVGIPLAERLLVLILASLLHGFEWKLPEGSTLDLEEK 420
           NE MYDF+GNKIEFCPFG+GKK C GIPLAERLLVLILASLLHGFEWKLPEGS LDLEEK
Sbjct: 434 NEKMYDFSGNKIEFCPFGSGKKSCAGIPLAERLLVLILASLLHGFEWKLPEGSKLDLEEK 493

Query: 421 FGIVSKKLNPLVVIPTPRLSNLELYNMM 449
           FGIV++KLNPLVVIPTPRLSNLELYN M
Sbjct: 494 FGIVTRKLNPLVVIPTPRLSNLELYNTM 521

BLAST of Cucsa.302220.1 vs. NCBI nr
Match: gi|659073582|ref|XP_008437139.1| (PREDICTED: 7-ethoxycoumarin O-deethylase-like isoform X1 [Cucumis melo])

HSP 1 Score: 733.4 bits (1892), Expect = 2.4e-208
Identity = 361/448 (80.58%), Postives = 401/448 (89.51%), Query Frame = 1

Query: 1   MYGPIFKLRLGTKLCVVLTSPSSVNEVLRHQETVFDNRDATVSALLATYGGVDIVFSQNE 60
           +YG I KL LGTKLC+VLTSPSSVNE LRHQET+F  RD TV ALL++YGG DIVF+Q+E
Sbjct: 79  VYGSILKLHLGTKLCIVLTSPSSVNEALRHQETIFSIRDTTVVALLSSYGGSDIVFTQDE 138

Query: 61  SIWKKLRKIFARKMLSKSNLDASYPLRRREVRKIIKGVLESAGTPIDISKLSLMAAVKSV 120
           + WKKLRKIF RKMLSKSNLDASY LRR+EVRK+IKGV ESAGTPIDI KL  +AA+KSV
Sbjct: 139 NNWKKLRKIFTRKMLSKSNLDASYSLRRQEVRKVIKGVFESAGTPIDIGKLGFVAALKSV 198

Query: 121 MAMTWGGSRGLIGVDGADLEAKFMEVVNELMVLLGTPNLSDLFPVLGGLDLQGIGRKMKK 180
           MAMTWGGS GLIGVDG DL+AKF E V+E+MVLL TPNLSDLFPVLG  DLQG+ RKMKK
Sbjct: 199 MAMTWGGSGGLIGVDGTDLDAKFREAVDEMMVLLATPNLSDLFPVLGRFDLQGVVRKMKK 258

Query: 181 VMNVFDGIFNSAIEEQRKRGGDGMENRGFLQLLLEVMEGEDSSESITDKELKALLVDIII 240
           VM VFD IFNSAIEEQRKRGG+GMENRGFLQ LLE+MEGEDSSESIT+  LKALL+DI+I
Sbjct: 259 VMCVFDEIFNSAIEEQRKRGGNGMENRGFLQFLLEIMEGEDSSESITENNLKALLMDIVI 318

Query: 241 GGTDTTSTTIEWTIAELIQQPNIMKKVKEELTKVVGLNQMVEEFHLSKLHYLDAAIKETL 300
           GGTDTTSTT+EW I+ELI+QPN M KV EELTKVVGLNQMVEEFHL KL++LDA +KETL
Sbjct: 319 GGTDTTSTTVEWAISELIKQPNTMNKVIEELTKVVGLNQMVEEFHLPKLNFLDAVVKETL 378

Query: 301 RLHPPVPLLVPRTTNQRTTLEGYTIPKSSTIYFNIWAIQRDPKIWDNPLDFMPERFLNES 360
           RLHPP+PLLVPRT++Q TT   YTIPK STIYFNIWAIQRDPK+WDNPL+F PERFLNES
Sbjct: 379 RLHPPLPLLVPRTSSQTTTFGEYTIPKGSTIYFNIWAIQRDPKVWDNPLNFEPERFLNES 438

Query: 361 NENMYDFTGNKIEFCPFGAGKKLCVGIPLAERLLVLILASLLHGFEWKLPEGSTLDLEEK 420
           +EN+YDFTGN+IEFCPFG+GKK C GIPLA+RLLVLILASLLHGFEWKLPEGS  D+EEK
Sbjct: 439 SENLYDFTGNRIEFCPFGSGKKSCAGIPLAKRLLVLILASLLHGFEWKLPEGSKFDMEEK 498

Query: 421 FGIVSKKLNPLVVIPTPRLSNLELYNMM 449
           FGIV+KKLNPLV IPTPRL NLELYN+M
Sbjct: 499 FGIVTKKLNPLVTIPTPRLPNLELYNIM 526

BLAST of Cucsa.302220.1 vs. NCBI nr
Match: gi|659073584|ref|XP_008437141.1| (PREDICTED: 7-ethoxycoumarin O-deethylase-like isoform X2 [Cucumis melo])

HSP 1 Score: 733.4 bits (1892), Expect = 2.4e-208
Identity = 361/448 (80.58%), Postives = 401/448 (89.51%), Query Frame = 1

Query: 1   MYGPIFKLRLGTKLCVVLTSPSSVNEVLRHQETVFDNRDATVSALLATYGGVDIVFSQNE 60
           +YG I KL LGTKLC+VLTSPSSVNE LRHQET+F  RD TV ALL++YGG DIVF+Q+E
Sbjct: 73  VYGSILKLHLGTKLCIVLTSPSSVNEALRHQETIFSIRDTTVVALLSSYGGSDIVFTQDE 132

Query: 61  SIWKKLRKIFARKMLSKSNLDASYPLRRREVRKIIKGVLESAGTPIDISKLSLMAAVKSV 120
           + WKKLRKIF RKMLSKSNLDASY LRR+EVRK+IKGV ESAGTPIDI KL  +AA+KSV
Sbjct: 133 NNWKKLRKIFTRKMLSKSNLDASYSLRRQEVRKVIKGVFESAGTPIDIGKLGFVAALKSV 192

Query: 121 MAMTWGGSRGLIGVDGADLEAKFMEVVNELMVLLGTPNLSDLFPVLGGLDLQGIGRKMKK 180
           MAMTWGGS GLIGVDG DL+AKF E V+E+MVLL TPNLSDLFPVLG  DLQG+ RKMKK
Sbjct: 193 MAMTWGGSGGLIGVDGTDLDAKFREAVDEMMVLLATPNLSDLFPVLGRFDLQGVVRKMKK 252

Query: 181 VMNVFDGIFNSAIEEQRKRGGDGMENRGFLQLLLEVMEGEDSSESITDKELKALLVDIII 240
           VM VFD IFNSAIEEQRKRGG+GMENRGFLQ LLE+MEGEDSSESIT+  LKALL+DI+I
Sbjct: 253 VMCVFDEIFNSAIEEQRKRGGNGMENRGFLQFLLEIMEGEDSSESITENNLKALLMDIVI 312

Query: 241 GGTDTTSTTIEWTIAELIQQPNIMKKVKEELTKVVGLNQMVEEFHLSKLHYLDAAIKETL 300
           GGTDTTSTT+EW I+ELI+QPN M KV EELTKVVGLNQMVEEFHL KL++LDA +KETL
Sbjct: 313 GGTDTTSTTVEWAISELIKQPNTMNKVIEELTKVVGLNQMVEEFHLPKLNFLDAVVKETL 372

Query: 301 RLHPPVPLLVPRTTNQRTTLEGYTIPKSSTIYFNIWAIQRDPKIWDNPLDFMPERFLNES 360
           RLHPP+PLLVPRT++Q TT   YTIPK STIYFNIWAIQRDPK+WDNPL+F PERFLNES
Sbjct: 373 RLHPPLPLLVPRTSSQTTTFGEYTIPKGSTIYFNIWAIQRDPKVWDNPLNFEPERFLNES 432

Query: 361 NENMYDFTGNKIEFCPFGAGKKLCVGIPLAERLLVLILASLLHGFEWKLPEGSTLDLEEK 420
           +EN+YDFTGN+IEFCPFG+GKK C GIPLA+RLLVLILASLLHGFEWKLPEGS  D+EEK
Sbjct: 433 SENLYDFTGNRIEFCPFGSGKKSCAGIPLAKRLLVLILASLLHGFEWKLPEGSKFDMEEK 492

Query: 421 FGIVSKKLNPLVVIPTPRLSNLELYNMM 449
           FGIV+KKLNPLV IPTPRL NLELYN+M
Sbjct: 493 FGIVTKKLNPLVTIPTPRLPNLELYNIM 520

BLAST of Cucsa.302220.1 vs. NCBI nr
Match: gi|659073592|ref|XP_008437145.1| (PREDICTED: 7-ethoxycoumarin O-deethylase-like [Cucumis melo])

HSP 1 Score: 596.3 bits (1536), Expect = 4.5e-167
Identity = 296/447 (66.22%), Postives = 357/447 (79.87%), Query Frame = 1

Query: 1   MYGPIFKLRLGTKLCVVLTSPSSVNEVLRHQETVFDNRDATVSALLATYGGVDIVFSQNE 60
           +YGP++KLRLG KLC+VL SP+SV E   HQET+F NRD TV ALL+ YGG  IVF+Q+E
Sbjct: 112 IYGPVYKLRLGPKLCIVLNSPASVKEAFHHQETLFPNRDTTVCALLSGYGGSGIVFAQDE 171

Query: 61  SIWKKLRKIFARKMLSKSNLDASYPLRRREVRKIIKGVLESAGTPIDISKLSLMAAVKSV 120
             WKKLRKIF RKM+SKSNLDASY +RR+EVRK+IKGV ES GT IDI K+S  A +KSV
Sbjct: 172 GDWKKLRKIFTRKMISKSNLDASYFVRRQEVRKVIKGVFESVGTQIDIGKVSFHATMKSV 231

Query: 121 MAMTWGGSRGLIGVDGADLEAKFMEVVNELMVLLGTPNLSDLFPVLGGLDLQGIGRKMKK 180
           +AMTWG S  L+G DG  LE KF E+++ELMVLLG+PN+SD+FPVLG  DLQGIGR+ KK
Sbjct: 232 LAMTWGDSGRLMGEDGVGLEEKFREMMDELMVLLGSPNVSDVFPVLGRFDLQGIGRRTKK 291

Query: 181 VMNVFDGIFNSAIEEQRKRG--GDGMENRGFLQLLLEVMEGEDSSESITDKELKALLVDI 240
           VM V DGI N +IEEQRK G  G+G    G+LQLLLE+ + ED+SE IT+ +LKALL+DI
Sbjct: 292 VMGVLDGILNFSIEEQRKMGGKGNGEGKGGYLQLLLELQDNEDNSECITNDQLKALLLDI 351

Query: 241 IIGGTDTTSTTIEWTIAELIQQPNIMKKVKEELTKVVGLNQMVEEFHLSKLHYLDAAIKE 300
           +IGGT+TT+TT+EW +AEL+Q PN +KKVKEEL KV+GLN +VEEFH SKL YL+A IKE
Sbjct: 352 VIGGTETTATTVEWAMAELMQHPNTIKKVKEELKKVIGLNAVVEEFHFSKLCYLNAVIKE 411

Query: 301 TLRLHPPVPLLVPRTTNQRTTLEGYTIPKSSTIYFNIWAIQRDPKIWDNPLDFMPERFLN 360
           TLRLHPP+ LLVPR+    TTL GY IPK ST+YFN+WAIQRDPK WDNP  FMPERF+N
Sbjct: 412 TLRLHPPIFLLVPRSLTSSTTLGGYYIPKDSTLYFNLWAIQRDPKTWDNPSKFMPERFIN 471

Query: 361 ESNENMYDFTGNKIEFCPFGAGKKLCVGIPLAERLLVLILASLLHGFEWKLPEGSTLDLE 420
            +N+       N +EF PFG GKK C G+ LAER+L+ ILASLLH F+W+LP+ S +D +
Sbjct: 472 NNND-------NAMEFYPFGYGKKSCAGVALAERMLMFILASLLHSFDWELPKDSVIDFK 531

Query: 421 EKFGIVSKKLNPLVVIPTPRLSNLELY 446
           EKFGIV+KKLNPLV IPTP LSN +LY
Sbjct: 532 EKFGIVNKKLNPLVAIPTPTLSNSDLY 551

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
F3PH_PETHY1.2e-7936.63Flavonoid 3'-monooxygenase OS=Petunia hybrida GN=CYP75B2 PE=2 SV=1[more]
F3PH_ARATH1.3e-7837.98Flavonoid 3'-monooxygenase OS=Arabidopsis thaliana GN=CYP75B1 PE=1 SV=1[more]
C76BA_SWEMU2.3e-7837.53Geraniol 8-hydroxylase OS=Swertia mussotii GN=CYP76B10 PE=1 SV=1[more]
C76C2_ARATH2.8e-7635.41Cytochrome P450 76C2 OS=Arabidopsis thaliana GN=CYP76C2 PE=2 SV=1[more]
C76B6_CATRO3.7e-7635.81Geraniol 8-hydroxylase OS=Catharanthus roseus GN=CYP76B6 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
M5WDL9_PRUPE7.3e-14053.76Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa019218mg PE=3 SV=1[more]
M5WSD8_PRUPE1.2e-13452.43Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa019849mg PE=3 SV=1[more]
A0A067FFE7_CITSI2.7e-13452.49Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g009481mg PE=3 SV=1[more]
V4U067_9ROSI1.3e-13352.92Uncharacterized protein OS=Citrus clementina GN=CICLE_v10014858mg PE=3 SV=1[more]
A0A067FBZ9_CITSI1.3e-13352.92Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g009495mg PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT4G12320.11.1e-11044.37 cytochrome P450, family 706, subfamily A, polypeptide 6[more]
AT4G12300.13.2e-11045.01 cytochrome P450, family 706, subfamily A, polypeptide 4[more]
AT4G12310.14.6e-10943.71 cytochrome P450, family 706, subfamily A, polypeptide 5[more]
AT5G44620.14.1e-10241.72 cytochrome P450, family 706, subfamily A, polypeptide 3[more]
AT4G12330.12.0e-10141.02 cytochrome P450, family 706, subfamily A, polypeptide 7[more]
Match NameE-valueIdentityDescription
gi|778699919|ref|XP_004147610.2|1.1e-253100.00PREDICTED: geraniol 8-hydroxylase-like [Cucumis sativus][more]
gi|659073586|ref|XP_008437142.1|4.9e-21483.93PREDICTED: flavonoid 3'-monooxygenase-like [Cucumis melo][more]
gi|659073582|ref|XP_008437139.1|2.4e-20880.58PREDICTED: 7-ethoxycoumarin O-deethylase-like isoform X1 [Cucumis melo][more]
gi|659073584|ref|XP_008437141.1|2.4e-20880.58PREDICTED: 7-ethoxycoumarin O-deethylase-like isoform X2 [Cucumis melo][more]
gi|659073592|ref|XP_008437145.1|4.5e-16766.22PREDICTED: 7-ethoxycoumarin O-deethylase-like [Cucumis melo][more]
The following terms have been associated with this mRNA:
Vocabulary: INTERPRO
TermDefinition
IPR001128Cyt_P450
IPR002401Cyt_P450_E_grp-I
IPR017972Cyt_P450_CS
Vocabulary: Molecular Function
TermDefinition
GO:0016705oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
GO:0005506iron ion binding
GO:0020037heme binding
Vocabulary: Biological Process
TermDefinition
GO:0055114oxidation-reduction process
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055114 oxidation-reduction process
cellular_component GO:0005575 cellular_component
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Cucsa.302220Cucsa.302220gene


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Cucsa.302220.1Cucsa.302220.1-proteinpolypeptide


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsa.302220.1.five_prime_UTR.1Cucsa.302220.1.five_prime_UTR.1five_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsa.302220.1.CDS.1Cucsa.302220.1.CDS.1CDS
Cucsa.302220.1.CDS.2Cucsa.302220.1.CDS.2CDS


Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001128Cytochrome P450PRINTSPR00385P450coord: 294..305
score: 4.8E-13coord: 375..384
score: 4.8E-13coord: 384..395
score: 4.8E-13coord: 241..258
score: 4.8
IPR001128Cytochrome P450GENE3DG3DSA:1.10.630.10coord: 2..438
score: 1.3E
IPR001128Cytochrome P450PFAMPF00067p450coord: 2..429
score: 5.0
IPR001128Cytochrome P450unknownSSF48264Cytochrome P450coord: 2..438
score: 8.77E
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 250..276
score: 1.9E-42coord: 384..407
score: 1.9E-42coord: 23..44
score: 1.9E-42coord: 230..247
score: 1.9E-42coord: 293..311
score: 1.9E-42coord: 334..358
score: 1.9E-42coord: 374..384
score: 1.9
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 377..386
scor
NoneNo IPR availablePANTHERPTHR24298FAMILY NOT NAMEDcoord: 1..442
score: 1.2E
NoneNo IPR availablePANTHERPTHR24298:SF136SUBFAMILY NOT NAMEDcoord: 1..442
score: 1.2E