Cucsa.302220 (gene) Cucumber (Gy14) v1

NameCucsa.302220
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionCytochrome P450, putative
Locationscaffold02951 : 419901 .. 422326 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
GCCGACACTCTCAATTCCCTTCCTCTTCTCATTTTGGTCCTTAGATTTCAACAAACACAGTCAAATTTCAACAGCACTTCTTTTTTTCTTCCTTTTCATTTTTGCTCTCTTATGGCTCCAATCCAAATTCCAGCATCCCCCTTTGCCTCCAGGCCCCCGTGGCCTGCCCCTGCTCGGTTATCTTCCATTTTTATCCACCAATCTCCACCACACATTCAACAATTTGGCCAAAATGTACGGCCCAATTTTCAAGCTCCGCCTCGGAACTAAGCTCTGCGTTGTTCTCACCTCCCCTTCCTCCGTCAATGAAGTCCTCCGTCACCAAGAAACTGTCTTCGACAACCGAGATGCCACCGTCAGCGCTCTTCTTGCCACCTACGGCGGAGTCGATATTGTATTCAGCCAGAACGAGAGCATTTGGAAGAAGCTGAGAAAAATCTTTGCCCGAAAAATGCTTAGCAAATCAAATCTTGATGCGTCGTATCCTTTGCGAAGACGAGAAGTGAGAAAAATAATTAAAGGCGTGTTGGAATCGGCGGGAACCCCAATTGATATCAGTAAATTGAGTTTGATGGCTGCTGTGAAATCGGTAATGGCGATGACGTGGGGTGGCTCAAGGGGACTGATCGGAGTGGATGGGGCTGATTTGGAAGCGAAGTTCATGGAAGTGGTGAATGAACTGATGGTGTTGCTTGGAACTCCAAATTTGTCGGATCTATTTCCGGTGTTGGGTGGGTTAGATTTGCAGGGAATTGGGAGGAAGATGAAGAAGGTGATGAATGTTTTTGATGGGATTTTTAATTCTGCCATTGAAGAACAGAGGAAAAGGGGAGGAGATGGGATGGAAAACAGAGGGTTTTTACAGTTACTGTTGGAGGTTATGGAGGGTGAGGATAGTTCAGAGTCCATTACCGATAAAGAACTCAAGGCTTTGCTAGTCGTATGCTTTCCCAATTACTTATCTTTTCATTGCCATTCCAATATGCTCTTTTTTACCCTTAAATGATTATTAGATTCTATTTAAATCCATACCATTCCAATTTCTAAAAATTAGTTCAAAATTATAATTATAATGAGCGAAGTTGTTTTAAATTACAAATAAAAAaTTTAAAATATTTATAAATATGCCAAAaTTTTATATGTTATTAGTGATAAATAGGTATCGATAGATATATATATATATATATAAGTGATAAACGCTAATaTTCTATCATTATTATATTTTGTAAATATTTTAGTTTATTTtATTGTAATTtAAAaTATAATTTtAAAAACAATTtATTTTtCTTGTAAtTTATTtTTtAAAAAAAaTTGTGATAATGCAAGCTCTATCTGAGTGGAGCTATATTATTTTAGGACAAGTATACCTGCCTTTTCTTTTATCACTTCTAATAATATTTGACTTCTTAATCAAATTTAAAaTTTAAAATTTTAAAATATTGTCCTAGTTTAAAAAATTAGGTTTTTTTtCCTATAAAAaCAAATTGGTATGGTTTTGTTTGAGTTAAAATTTTACATTTGTGCCCAATCATTTTTTtCTCATAATGGAATCTCAATTATGGCAATTTTCAATTTATAATACATATTAAAAGTCATTCCTTCGAATAAATTTTTtGAACAAGCTCTTGATAACTGTTATGAGATAGAAaaaGATAGATGAGTATGAGGTTTTtCCCCcTTTTTTTTTTttCCTGTTCTTTTTCtCTAATTGATTTTTTtATTGAATTTACATTAAGTAGTTCAATGTATTTAAGAACCTATGATCTCTTTTTAATGGTTTTGTATAGGACATCATCATTGGAGGAACAGACACAACATCAACTACAATTGAGTGGACAATCGCAGAATTGATACAACAACCAAACATAATGAAGAAAGTCAAAGAAGAATTAACAAAAGTTGTGGGTTTAAACCAAATGGTTGAAGAATTTCACTTATCTAAATTACATTATTTAGATGCAGCAATTAAAGAAACACTTCGTTTACACCCACCAGTACCTCTTTTAGTGCCACGGACGACTAACCAAAGAACCACCCTCGAAGGGTACACTATTCCAAAGAGCTCAACTATCTACTTCAATATTTGGGCCATCCAAAGAGACCCTAAAATTTGGGACAACCCATTAGACTTCATGCCTGAGAGATTTTTGAATGAATCTAATGAAAATATGTACGATTTTACTGGCAATAAGATAGAGTTTTGTCCATTTGGAGCAGGTAAAAAGTTATGTGTGGGGATTCCTCTAGCCGAGAGGTTGTTGGTTTTAATATTAGCTTCATTGTTGCATGGATTTGAATGGAAATTACCCGAGGGTTCAACGCTTGATTTGGAAGAAAAGTTTGGAATTGTCAGTAAGAAGTTGAATCCTTTGGTTGTTATTCCTACACCAAGGCTTTCCAACTTGGAGCTTTATAATATGATGTAG

mRNA sequence

GCCGACACTCTCAATTCCCTTCCTCTTCTCATTTTGGTCCTTAGATTTCAACAAACACAGTCAAATTTCAACAGCACTTCTTTTTTTCTTCCTTTTCATTTTTGCTCTCTTATGGCTCCAATCCAAATTCCAGCATCCCCCTTTGCCTCCAGGCCCCCGTGGCCTGCCCCTGCTCGGTTATCTTCCATTTTTATCCACCAATCTCCACCACACATTCAACAATTTGGCCAAAATGTACGGCCCAATTTTCAAGCTCCGCCTCGGAACTAAGCTCTGCGTTGTTCTCACCTCCCCTTCCTCCGTCAATGAAGTCCTCCGTCACCAAGAAACTGTCTTCGACAACCGAGATGCCACCGTCAGCGCTCTTCTTGCCACCTACGGCGGAGTCGATATTGTATTCAGCCAGAACGAGAGCATTTGGAAGAAGCTGAGAAAAATCTTTGCCCGAAAAATGCTTAGCAAATCAAATCTTGATGCGTCGTATCCTTTGCGAAGACGAGAAGTGAGAAAAATAATTAAAGGCGTGTTGGAATCGGCGGGAACCCCAATTGATATCAGTAAATTGAGTTTGATGGCTGCTGTGAAATCGGTAATGGCGATGACGTGGGGTGGCTCAAGGGGACTGATCGGAGTGGATGGGGCTGATTTGGAAGCGAAGTTCATGGAAGTGGTGAATGAACTGATGGTGTTGCTTGGAACTCCAAATTTGTCGGATCTATTTCCGGTGTTGGGTGGGTTAGATTTGCAGGGAATTGGGAGGAAGATGAAGAAGGTGATGAATGTTTTTGATGGGATTTTTAATTCTGCCATTGAAGAACAGAGGAAAAGGGGAGGAGATGGGATGGAAAACAGAGGGTTTTTACAGTTACTGTTGGAGGTTATGGAGGGTGAGGATAGTTCAGAGTCCATTACCGATAAAGAACTCAAGGCTTTGCTAGTCGACATCATCATTGGAGGAACAGACACAACATCAACTACAATTGAGTGGACAATCGCAGAATTGATACAACAACCAAACATAATGAAGAAAGTCAAAGAAGAATTAACAAAAGTTGTGGGTTTAAACCAAATGGTTGAAGAATTTCACTTATCTAAATTACATTATTTAGATGCAGCAATTAAAGAAACACTTCGTTTACACCCACCAGTACCTCTTTTAGTGCCACGGACGACTAACCAAAGAACCACCCTCGAAGGGTACACTATTCCAAAGAGCTCAACTATCTACTTCAATATTTGGGCCATCCAAAGAGACCCTAAAATTTGGGACAACCCATTAGACTTCATGCCTGAGAGATTTTTGAATGAATCTAATGAAAATATGTACGATTTTACTGGCAATAAGATAGAGTTTTGTCCATTTGGAGCAGGTAAAAAGTTATGTGTGGGGATTCCTCTAGCCGAGAGGTTGTTGGTTTTAATATTAGCTTCATTGTTGCATGGATTTGAATGGAAATTACCCGAGGGTTCAACGCTTGATTTGGAAGAAAAGTTTGGAATTGTCAGTAAGAAGTTGAATCCTTTGGTTGTTATTCCTACACCAAGGCTTTCCAACTTGGAGCTTTATAATATGATGTAG

Coding sequence (CDS)

ATGTACGGCCCAATTTTCAAGCTCCGCCTCGGAACTAAGCTCTGCGTTGTTCTCACCTCCCCTTCCTCCGTCAATGAAGTCCTCCGTCACCAAGAAACTGTCTTCGACAACCGAGATGCCACCGTCAGCGCTCTTCTTGCCACCTACGGCGGAGTCGATATTGTATTCAGCCAGAACGAGAGCATTTGGAAGAAGCTGAGAAAAATCTTTGCCCGAAAAATGCTTAGCAAATCAAATCTTGATGCGTCGTATCCTTTGCGAAGACGAGAAGTGAGAAAAATAATTAAAGGCGTGTTGGAATCGGCGGGAACCCCAATTGATATCAGTAAATTGAGTTTGATGGCTGCTGTGAAATCGGTAATGGCGATGACGTGGGGTGGCTCAAGGGGACTGATCGGAGTGGATGGGGCTGATTTGGAAGCGAAGTTCATGGAAGTGGTGAATGAACTGATGGTGTTGCTTGGAACTCCAAATTTGTCGGATCTATTTCCGGTGTTGGGTGGGTTAGATTTGCAGGGAATTGGGAGGAAGATGAAGAAGGTGATGAATGTTTTTGATGGGATTTTTAATTCTGCCATTGAAGAACAGAGGAAAAGGGGAGGAGATGGGATGGAAAACAGAGGGTTTTTACAGTTACTGTTGGAGGTTATGGAGGGTGAGGATAGTTCAGAGTCCATTACCGATAAAGAACTCAAGGCTTTGCTAGTCGACATCATCATTGGAGGAACAGACACAACATCAACTACAATTGAGTGGACAATCGCAGAATTGATACAACAACCAAACATAATGAAGAAAGTCAAAGAAGAATTAACAAAAGTTGTGGGTTTAAACCAAATGGTTGAAGAATTTCACTTATCTAAATTACATTATTTAGATGCAGCAATTAAAGAAACACTTCGTTTACACCCACCAGTACCTCTTTTAGTGCCACGGACGACTAACCAAAGAACCACCCTCGAAGGGTACACTATTCCAAAGAGCTCAACTATCTACTTCAATATTTGGGCCATCCAAAGAGACCCTAAAATTTGGGACAACCCATTAGACTTCATGCCTGAGAGATTTTTGAATGAATCTAATGAAAATATGTACGATTTTACTGGCAATAAGATAGAGTTTTGTCCATTTGGAGCAGGTAAAAAGTTATGTGTGGGGATTCCTCTAGCCGAGAGGTTGTTGGTTTTAATATTAGCTTCATTGTTGCATGGATTTGAATGGAAATTACCCGAGGGTTCAACGCTTGATTTGGAAGAAAAGTTTGGAATTGTCAGTAAGAAGTTGAATCCTTTGGTTGTTATTCCTACACCAAGGCTTTCCAACTTGGAGCTTTATAATATGATGTAG

Protein sequence

MYGPIFKLRLGTKLCVVLTSPSSVNEVLRHQETVFDNRDATVSALLATYGGVDIVFSQNESIWKKLRKIFARKMLSKSNLDASYPLRRREVRKIIKGVLESAGTPIDISKLSLMAAVKSVMAMTWGGSRGLIGVDGADLEAKFMEVVNELMVLLGTPNLSDLFPVLGGLDLQGIGRKMKKVMNVFDGIFNSAIEEQRKRGGDGMENRGFLQLLLEVMEGEDSSESITDKELKALLVDIIIGGTDTTSTTIEWTIAELIQQPNIMKKVKEELTKVVGLNQMVEEFHLSKLHYLDAAIKETLRLHPPVPLLVPRTTNQRTTLEGYTIPKSSTIYFNIWAIQRDPKIWDNPLDFMPERFLNESNENMYDFTGNKIEFCPFGAGKKLCVGIPLAERLLVLILASLLHGFEWKLPEGSTLDLEEKFGIVSKKLNPLVVIPTPRLSNLELYNMM*
BLAST of Cucsa.302220 vs. Swiss-Prot
Match: F3PH_PETHY (Flavonoid 3'-monooxygenase OS=Petunia hybrida GN=CYP75B2 PE=2 SV=1)

HSP 1 Score: 298.5 bits (763), Expect = 1.2e-79
Identity = 163/445 (36.63%), Postives = 257/445 (57.75%), Query Frame = 1

Query: 2   YGPIFKLRLGTKLCVVLTSPSSVNEVLRHQETVFDNRDATVSALLATYGGVDIVFSQNES 61
           YGP+  L++G    VV  S S   + L+  +  F +R     A    Y   D+VF+    
Sbjct: 64  YGPLMYLKMGFVDVVVAASASVAAQFLKTHDANFSSRPPNSGAEHMAYNYQDLVFAPYGP 123

Query: 62  IWKKLRKIFARKMLSKSNLDASYPLRRREVRKIIKGVLESAGTPIDISKLSLMAAVKSVM 121
            W+ LRKI +  + S   LD    +R+ EV+ + + +  +   P+ + +L L     + +
Sbjct: 124 RWRMLRKICSVHLFSTKALDDFRHVRQDEVKTLTRALASAGQKPVKLGQL-LNVCTTNAL 183

Query: 122 AMTWGGSRGLIGVDGA-DLE---AKFMEVVNELMVLLGTPNLSDLFPVLGGLDLQGIGRK 181
           A    G R  +  DG+ D++   A+F  +V E+MV+ G  N+ D  P L  LD+QG+  K
Sbjct: 184 ARVMLGKR--VFADGSGDVDPQAAEFKSMVVEMMVVAGVFNIGDFIPQLNWLDIQGVAAK 243

Query: 182 MKKVMNVFDGIFNSAIEEQRKRGGDGMENRGFLQLLLEVMEGEDSSESITDKELKALLVD 241
           MKK+   FD      +EE + +    M++     + L+  + ++    +TD E+KALL++
Sbjct: 244 MKKLHARFDAFLTDILEEHKGKIFGEMKDLLSTLISLKNDDADNDGGKLTDTEIKALLLN 303

Query: 242 IIIGGTDTTSTTIEWTIAELIQQPNIMKKVKEELTKVVGLNQMVEEFHLSKLHYLDAAIK 301
           + + GTDT+S+T+EW IAELI+ P I+ + ++E+ KVVG +++V E  L++L YL+A +K
Sbjct: 304 LFVAGTDTSSSTVEWAIAELIRNPKILAQAQQEIDKVVGRDRLVGELDLAQLTYLEAIVK 363

Query: 302 ETLRLHPPVPLLVPRTTNQRTTLEGYTIPKSSTIYFNIWAIQRDPKIWDNPLDFMPERFL 361
           ET RLHP  PL +PR  ++   + GY IPK ST+  N+WAI RDP  W +PL+F PERFL
Sbjct: 364 ETFRLHPSTPLSLPRIASESCEINGYFIPKGSTLLLNVWAIARDPNAWADPLEFRPERFL 423

Query: 362 NESNENMYDFTGNKIEFCPFGAGKKLCVGIPLAERLLVLILASLLHGFEWKLPEG---ST 421
               +   D  GN  E  PFGAG+++C G+ L  R++ L++A+L+H F W L  G     
Sbjct: 424 PGGEKPKVDVRGNDFEVIPFGAGRRICAGMNLGIRMVQLMIATLIHAFNWDLVSGQLPEM 483

Query: 422 LDLEEKFGIVSKKLNPLVVIPTPRL 440
           L++EE +G+  ++ +PLVV P PRL
Sbjct: 484 LNMEEAYGLTLQRADPLVVHPRPRL 505

BLAST of Cucsa.302220 vs. Swiss-Prot
Match: F3PH_ARATH (Flavonoid 3'-monooxygenase OS=Arabidopsis thaliana GN=CYP75B1 PE=1 SV=1)

HSP 1 Score: 295.0 bits (754), Expect = 1.3e-78
Identity = 169/445 (37.98%), Postives = 250/445 (56.18%), Query Frame = 1

Query: 2   YGPIFKLRLGTKLCVVLTSPSSVNEVLRHQETVFDNRDATVSALLATYGGVDIVFSQNES 61
           YGPI  LRLG    VV  S S   + L+  +  F +R     A    Y   D+VF+    
Sbjct: 64  YGPILHLRLGFVDVVVAASKSVAEQFLKIHDANFASRPPNSGAKHMAYNYQDLVFAPYGH 123

Query: 62  IWKKLRKIFARKMLSKSNLDASYPLRRREVRKIIKGVLESAGTPIDISKLSLMAAVKSVM 121
            W+ LRKI +  + S   L+    +R+ EV  + + ++     P+++ +L  M  V ++ 
Sbjct: 124 RWRLLRKISSVHLFSAKALEDFKHVRQEEVGTLTRELVRVGTKPVNLGQLVNMCVVNALG 183

Query: 122 AMTWGGSRGLIGVDGADLEAKFMEVVNELMVLLGTPNLSDLFPVLGGLDLQGIGRKMKKV 181
               G  R L G D      +F  +V E+M L G  N+ D  P L  LDLQG+  KMK++
Sbjct: 184 REMIG--RRLFGADADHKADEFRSMVTEMMALAGVFNIGDFVPSLDWLDLQGVAGKMKRL 243

Query: 182 MNVFDGIFNSAIEEQRKRGGDGMENRGFLQLLLEVMEGED---SSESITDKELKALLVDI 241
              FD   +S ++E    G D  ++   L  L+  ++G D      S+TD E+KALL+++
Sbjct: 244 HKRFDAFLSSILKEHEMNGQD-QKHTDMLSTLIS-LKGTDLDGDGGSLTDTEIKALLLNM 303

Query: 242 IIGGTDTTSTTIEWTIAELIQQPNIMKKVKEELTKVVGLNQMVEEFHLSKLHYLDAAIKE 301
              GTDT+++T++W IAELI+ P+IM K +EEL  VVG ++ V E  +++L YL A IKE
Sbjct: 304 FTAGTDTSASTVDWAIAELIRHPDIMVKAQEELDIVVGRDRPVNESDIAQLPYLQAVIKE 363

Query: 302 TLRLHPPVPLLVPRTTNQRTTLEGYTIPKSSTIYFNIWAIQRDPKIWDNPLDFMPERFLN 361
             RLHPP PL +P   ++   + GY IPK ST+  NIWAI RDP  W +PL F PERFL 
Sbjct: 364 NFRLHPPTPLSLPHIASESCEINGYHIPKGSTLLTNIWAIARDPDQWSDPLAFKPERFLP 423

Query: 362 ESNENMYDFTGNKIEFCPFGAGKKLCVGIPLAERLLVLILASLLHGFEWKLPEGST---L 421
              ++  D  G+  E  PFGAG+++C G+ L  R +  + A+L+ GF+W+L  G T   L
Sbjct: 424 GGEKSGVDVKGSDFELIPFGAGRRICAGLSLGLRTIQFLTATLVQGFDWELAGGVTPEKL 483

Query: 422 DLEEKFGIVSKKLNPLVVIPTPRLS 441
           ++EE +G+  ++  PLVV P PRL+
Sbjct: 484 NMEESYGLTLQRAVPLVVHPKPRLA 504

BLAST of Cucsa.302220 vs. Swiss-Prot
Match: C76BA_SWEMU (Geraniol 8-hydroxylase OS=Swertia mussotii GN=CYP76B10 PE=1 SV=1)

HSP 1 Score: 294.3 bits (752), Expect = 2.3e-78
Identity = 167/445 (37.53%), Postives = 260/445 (58.43%), Query Frame = 1

Query: 2   YGPIFKLRLGTKLCVVLTSPSSVNEVLRHQETVFDNRDATVSALLATYGGVDIVFSQNES 61
           +GPI  L+LG    +V+TS     EVL+ Q+  F +R    +          +++    S
Sbjct: 63  HGPIMGLQLGQVTTIVVTSSGMAKEVLQKQDLAFSSRSIPNAIHAHDQYKYSVIWLPVAS 122

Query: 62  IWKKLRKIFARKMLSKSNLDASYPLRRREVRKIIKGVLESA--GTPIDISKLSLMAAVKS 121
            W+ LRK     M S + LDA+  LR R+V+++I    +S+  G  ID+ + +   ++  
Sbjct: 123 RWRGLRKALNSNMFSGNRLDANQHLRSRKVQELIAYCRKSSQTGDAIDVGRAAFRTSLNL 182

Query: 122 VMAMTWGGSRGLIGVDGADLEAKFMEVVNELMVLLGTPNLSDLFPVLGGLDLQGIGRKMK 181
           +    +  S+ L     +D   +F ++V  +MV  G PNL D FP+L  +D QGI ++M 
Sbjct: 183 LSNTMF--SKDLTD-PYSDSAKEFKDLVWNVMVEAGKPNLVDYFPLLDKVDPQGIRKRMT 242

Query: 182 ----KVMNVFDGIFNSAIEEQRKRGGDGMENRGFLQLLLEVMEGEDSSESITDKELKALL 241
               K++ +F G+ +  +++++ +G     N   L +LL     E+S E I    ++ + 
Sbjct: 243 IHFGKILELFGGLIDERLQQKKAKG----VNDDVLDVLLTT--SEESPEEIDRTHIQRMC 302

Query: 242 VDIIIGGTDTTSTTIEWTIAELIQQPNIMKKVKEELTKVVGLNQMVEEFHLSKLHYLDAA 301
           +D+ + GTDTTS+T+EW ++E+++ P  MK  + EL +V+G  + VEE  L++L YL  A
Sbjct: 303 LDLFVAGTDTTSSTLEWAMSEMLKNPEKMKAAQAELAQVIGKGKAVEEADLARLPYLRCA 362

Query: 302 IKETLRLHPPVPLLVPRTTNQRTTLEGYTIPKSSTIYFNIWAIQRDPKIWDNPLDFMPER 361
           IKETLR+HPPVPLL+PR T Q   + GYT+PK+S +  N+WAI RD  IW +PL F PER
Sbjct: 363 IKETLRIHPPVPLLIPRRTEQEVEVCGYTVPKNSQVLVNVWAISRDDAIWKDPLSFKPER 422

Query: 362 FLNESNENMYDFTGNKIEFCPFGAGKKLCVGIPLAERLLVLILASLLHGFEWKLPEG--- 421
           FL    E+  +  G   E  PFGAG+++C G+PLA R++ ++L SLL+ F+WKL  G   
Sbjct: 423 FL----ESELEMRGKDFELIPFGAGRRICPGLPLAVRMVPVMLGSLLNSFDWKLEGGIAP 482

Query: 422 STLDLEEKFGIVSKKLNPLVVIPTP 438
             LD+EEKFGI  +K +PL  + TP
Sbjct: 483 KDLDMEEKFGITLQKAHPLRAVATP 494

BLAST of Cucsa.302220 vs. Swiss-Prot
Match: C76C2_ARATH (Cytochrome P450 76C2 OS=Arabidopsis thaliana GN=CYP76C2 PE=2 SV=1)

HSP 1 Score: 287.3 bits (734), Expect = 2.8e-76
Identity = 159/449 (35.41%), Postives = 256/449 (57.02%), Query Frame = 1

Query: 2   YGPIFKLRLGTKLCVVLTSPSSVNEVLRHQETVFDNRDATVSALLATYGGVDIVF-SQNE 61
           YGPI  L+ G+   VV+TSP +  EVLR  + +  +R  T S     +  V +V+   + 
Sbjct: 69  YGPIMSLKFGSLNTVVVTSPEAAREVLRTYDQILSSRTPTNSIRSINHDKVSVVWLPPSS 128

Query: 62  SIWKKLRKIFARKMLSKSNLDASYPLRRREVRKIIKGVLESAGTP--IDISKLSLMAAVK 121
           S W+ LRK+ A ++ S   ++A+  LR  +V++++  + ES+     +DIS+ + + A+ 
Sbjct: 129 SRWRLLRKLSATQLFSPQRIEATKTLRENKVKELVSFMSESSEREEAVDISRATFITALN 188

Query: 122 SVMAMTWGGSRGLIGVDGADLEAKFMEVVNELMVLLGTPNLSDLFPVLGGLDLQGIGRKM 181
            +  + +      +G   ++    F + V  +M  +G P+ ++ FP LG LDLQG  + +
Sbjct: 189 IISNILFSVD---LGNYDSNKSGVFQDTVIGVMEAVGNPDAANFFPFLGFLDLQGNRKTL 248

Query: 182 K----KVMNVFDGIFNSAIEEQ--RKRGGDGMENRGFLQLLLEVMEGEDSSESITDKELK 241
           K    ++  VF G  ++ + E+  R      +  R F+ +LL++ EG+++  +  D  + 
Sbjct: 249 KACSERLFKVFRGFIDAKLAEKSLRDTNSKDVRERDFVDVLLDLTEGDEAELNTND--IV 308

Query: 242 ALLVDIIIGGTDTTSTTIEWTIAELIQQPNIMKKVKEELTKVVGLNQMVEEFHLSKLHYL 301
            LL+D+   GTDT S+T+EW +AEL++ P  M K + E+  V+G   +VEE  +S L YL
Sbjct: 309 HLLLDLFGAGTDTNSSTVEWAMAELLRNPETMVKAQAEIDCVIGQKGVVEESDISALPYL 368

Query: 302 DAAIKETLRLHPPVPLLVPRTTNQRTTLEGYTIPKSSTIYFNIWAIQRDPKIWDNPLDFM 361
            A +KET RLHP  PLLVPR       + G+ +PK + ++ N+WAI RDP +W+N   F 
Sbjct: 369 QAVVKETFRLHPAAPLLVPRKAESDVEVLGFMVPKDTQVFVNVWAIGRDPNVWENSSRFK 428

Query: 362 PERFLNESNENMYDFTGNKIEFCPFGAGKKLCVGIPLAERLLVLILASLLHGFEWKLPEG 421
           PERFL +      D  G   E  PFGAG+++C G+PLA + + L+LASLL+ F+WKLP G
Sbjct: 429 PERFLGKD----IDLRGRDYELTPFGAGRRICPGLPLAVKTVPLMLASLLYSFDWKLPNG 488

Query: 422 ---STLDLEEKFGIVSKKLNPLVVIPTPR 439
                LD++E FG+   K NPL  +P  +
Sbjct: 489 VGSEDLDMDETFGLTLHKTNPLHAVPVKK 508

BLAST of Cucsa.302220 vs. Swiss-Prot
Match: C76B6_CATRO (Geraniol 8-hydroxylase OS=Catharanthus roseus GN=CYP76B6 PE=1 SV=1)

HSP 1 Score: 287.0 bits (733), Expect = 3.7e-76
Identity = 159/444 (35.81%), Postives = 257/444 (57.88%), Query Frame = 1

Query: 2   YGPIFKLRLGTKLCVVLTSPSSVNEVLRHQETVFDNRDATVSALLATYGGVDIVFSQNES 61
           +GPI  L+LG    +V++S +   EVL+ Q+  F +R    +          +V+    S
Sbjct: 61  HGPIMSLKLGQITTIVISSSTMAKEVLQKQDLAFSSRSVPNALHAHNQFKFSVVWLPVAS 120

Query: 62  IWKKLRKIFARKMLSKSNLDASYPLRRREVRKIIKGVLESA--GTPIDISKLSLMAAVKS 121
            W+ LRK+    + S + LDA+  LR R+V+++I    +++  G  +D+ + +   ++  
Sbjct: 121 RWRSLRKVLNSNIFSGNRLDANQHLRTRKVQELIAYCRKNSQSGEAVDVGRAAFRTSLNL 180

Query: 122 VMAMTWGGSRGLIGVDGADLEAKFMEVVNELMVLLGTPNLSDLFPVLGGLDLQGIGRKMK 181
           +  + +  S+ L     +D   +F ++V  +MV  G PNL D FP+L  +D QGI  +M 
Sbjct: 181 LSNLIF--SKDLTD-PYSDSAKEFKDLVWNIMVEAGKPNLVDFFPLLEKVDPQGIRHRMT 240

Query: 182 ----KVMNVFDGIFNSAIEEQRKRGGDGMENRGFLQLLLEVMEGEDSSESITDKELKALL 241
               +V+ +F G+ N  +E++R +G    E    L +LL     ++S E I    ++ + 
Sbjct: 241 IHFGEVLKLFGGLVNERLEQRRSKG----EKNDVLDVLLTT--SQESPEEIDRTHIERMC 300

Query: 242 VDIIIGGTDTTSTTIEWTIAELIQQPNIMKKVKEELTKVVGLNQMVEEFHLSKLHYLDAA 301
           +D+ + GTDTTS+T+EW ++E+++ P+ MKK ++EL +V+G  + +EE  +++L YL   
Sbjct: 301 LDLFVAGTDTTSSTLEWAMSEMLKNPDKMKKTQDELAQVIGRGKTIEESDINRLPYLRCV 360

Query: 302 IKETLRLHPPVPLLVPRTTNQRTTLEGYTIPKSSTIYFNIWAIQRDPKIWDNPLDFMPER 361
           +KETLR+HPPVP L+PR   Q   + GY +PK S +  N WAI RD  +WD+ L F PER
Sbjct: 361 MKETLRIHPPVPFLIPRKVEQSVEVCGYNVPKGSQVLVNAWAIGRDETVWDDALAFKPER 420

Query: 362 FLNESNENMYDFTGNKIEFCPFGAGKKLCVGIPLAERLLVLILASLLHGFEWKLPEG--- 421
           F+    E+  D  G   E  PFGAG+++C G+PLA R + L+L SLL+ F WKL  G   
Sbjct: 421 FM----ESELDIRGRDFELIPFGAGRRICPGLPLALRTVPLMLGSLLNSFNWKLEGGMAP 480

Query: 422 STLDLEEKFGIVSKKLNPLVVIPT 437
             LD+EEKFGI  +K +PL  +P+
Sbjct: 481 KDLDMEEKFGITLQKAHPLRAVPS 491

BLAST of Cucsa.302220 vs. TrEMBL
Match: M5WDL9_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa019218mg PE=3 SV=1)

HSP 1 Score: 505.4 bits (1300), Expect = 7.3e-140
Identity = 243/452 (53.76%), Postives = 337/452 (74.56%), Query Frame = 1

Query: 1   MYGPIFKLRLGTKLCVVLTSPSSVNEVLRHQETVFDNRDATVSALLATYGGVDIVFSQNE 60
           +YGPI+KL+LG+KLC VL+SPS V +VLR Q+T+F NRD T++A +A+YG  DI F    
Sbjct: 59  IYGPIYKLQLGSKLCFVLSSPSIVKQVLRDQDTLFANRDPTIAAQIASYGCTDIAFGPYG 118

Query: 61  SIWKKLRKIFARKMLSKSNLDASYPLRRREVRKIIKGVLESAGTPIDISKLSLMAAVKSV 120
             W++LRK+F   MLSK+NLDASY LR+ EV K I  V +  G+PID+ + + + ++ +V
Sbjct: 119 PGWRRLRKVFMSHMLSKANLDASYALRKEEVLKSISHVYDKVGSPIDLGQFAFLTSINTV 178

Query: 121 MAMTWGGSRGLIGVDGADLEAKFMEVVNELMVLLGTPNLSDLFPVLGGLDLQGIGRKMKK 180
           M M WGG+  L   +GADL A+F  VV E++ +LG PN+SD    L   D+QGI R+MK+
Sbjct: 179 MRMLWGGT--LEAENGADLGAEFRNVVAEIIEVLGKPNVSDFLTSLARFDVQGIARRMKQ 238

Query: 181 VMNVFDGIFNSAIEEQRKRG---GDGME----NRGFLQLLLEVMEGEDSSESITDKELKA 240
           + ++ + IF+SAIE Q+       +G+E     + FLQ+LLE+ + ED ++SIT ++LKA
Sbjct: 239 LQSMTEKIFDSAIERQKSEAVEKDEGLELKHERKDFLQILLELNDHEDGAKSITLQQLKA 298

Query: 241 LLVDIIIGGTDTTSTTIEWTIAELIQQPNIMKKVKEELTKVVGLNQMVEEFHLSKLHYLD 300
           LL+DI++GGTDTT+T +EW +AELI+  + +KKV+EEL +VVGL  MVEE H+ KLHYLD
Sbjct: 299 LLLDIVVGGTDTTATMVEWVMAELIKHQDDLKKVQEELKEVVGLKNMVEESHIPKLHYLD 358

Query: 301 AAIKETLRLHPPVPLLVPRTTNQRTTLEGYTIPKSSTIYFNIWAIQRDPKIWDNPLDFMP 360
           A IKET RLHP +PLL P   +Q TT+ GY IPK STI+ NIWAI RDP +WDNPL+F P
Sbjct: 359 AVIKETSRLHPALPLLAPHCPSQSTTIGGYNIPKGSTIFINIWAIHRDPNVWDNPLEFRP 418

Query: 361 ERFLNESNENMYDFTGNKIEFCPFGAGKKLCVGIPLAERLLVLILASLLHGFEWKLPEGS 420
           +RFLN+ + N +D+ GNK+E+ PFG+G+++C G+PLAE++++ +LAS LH FEW+LP  +
Sbjct: 419 KRFLNDPSSNNFDYKGNKLEYLPFGSGRRMCAGLPLAEKMMIYVLASFLHSFEWRLPTDA 478

Query: 421 TLDLEEKFGIVSKKLNPLVVIPTPRLSNLELY 446
             DL++KFGIV+KK+ PL+VIPTPRLS LELY
Sbjct: 479 KHDLQDKFGIVTKKMTPLIVIPTPRLSKLELY 508

BLAST of Cucsa.302220 vs. TrEMBL
Match: M5WSD8_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa019849mg PE=3 SV=1)

HSP 1 Score: 488.0 bits (1255), Expect = 1.2e-134
Identity = 237/452 (52.43%), Postives = 325/452 (71.90%), Query Frame = 1

Query: 1   MYGPIFKLRLGTKLCVVLTSPSSVNEVLRHQETVFDNRDATVSALLATYGGVDIVFSQNE 60
           +YGPI+KL+LG+KLC VL+SPS V +V+R Q+T+F N D  ++A + +YGG DI F    
Sbjct: 81  VYGPIYKLQLGSKLCFVLSSPSLVKQVVRDQDTLFANHDPIIAAQIISYGGTDIAFGSYG 140

Query: 61  SIWKKLRKIFARKMLSKSNLDASYPLRRREVRKIIKGVLESAGTPIDISKLSLMAAVKSV 120
             W++LRK+F   ++SK NLDA Y LR+ EV K I  +   + +PID  +++   AV ++
Sbjct: 141 PDWRRLRKVFVSHVMSKGNLDACYALRKEEVLKSIDHIYGKSDSPIDFGQVAFFTAVNTI 200

Query: 121 MAMTWGGSRGLIGVDGADLEAKFMEVVNELMVLLGTPNLSDLFPVLGGLDLQGIGRKMKK 180
           M M WGG+  L    G DL A+   VV E++ LLG  N+SD FP L   D+QGI R+ K+
Sbjct: 201 MRMLWGGT--LEAEKGTDLGAELRNVVAEMVELLGKGNISDFFPWLAKFDVQGIARRAKQ 260

Query: 181 VMNVFDGIFNSAIEEQRKRGGD---GM----ENRGFLQLLLEVMEGEDSSESITDKELKA 240
           V++V + I NSAIE+Q     +   G+    E +GFLQ LLE+ E  D +ES+T +++KA
Sbjct: 261 VLSVTEKILNSAIEKQMSEAAEQDGGLSLKHERKGFLQFLLELNEHGDDAESLTLQQIKA 320

Query: 241 LLVDIIIGGTDTTSTTIEWTIAELIQQPNIMKKVKEELTKVVGLNQMVEEFHLSKLHYLD 300
           LL DI+ GGTDTT+T +EW +AEL+QQP+ +KKV+EEL +VVGL  +VEE H+ KLHYLD
Sbjct: 321 LLTDIMAGGTDTTATMMEWVMAELMQQPDDLKKVQEELKEVVGLKNLVEESHIPKLHYLD 380

Query: 301 AAIKETLRLHPPVPLLVPRTTNQRTTLEGYTIPKSSTIYFNIWAIQRDPKIWDNPLDFMP 360
           A IKET RLHP +PLL P   +Q TT+ G+ IPK S+++ N+WAI RDP +WDNPL+F P
Sbjct: 381 AVIKETSRLHPALPLLAPHCPSQSTTIGGFKIPKGSSVFLNVWAIHRDPNVWDNPLEFRP 440

Query: 361 ERFLNESNENMYDFTGNKIEFCPFGAGKKLCVGIPLAERLLVLILASLLHGFEWKLPEGS 420
           +RFLN+   N   + GNK+E+ PFG+G+++C GIPLAER+++ +LAS LH FEW+LP  +
Sbjct: 441 KRFLNDPPTNNIHYNGNKLEYLPFGSGRRMCPGIPLAERMMIYVLASFLHSFEWRLPNDA 500

Query: 421 TLDLEEKFGIVSKKLNPLVVIPTPRLSNLELY 446
            LDL++KFGIV+KK+ PLVVIPTPRLS LELY
Sbjct: 501 KLDLQDKFGIVTKKMTPLVVIPTPRLSKLELY 530

BLAST of Cucsa.302220 vs. TrEMBL
Match: A0A067FFE7_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g009481mg PE=3 SV=1)

HSP 1 Score: 486.9 bits (1252), Expect = 2.7e-134
Identity = 242/461 (52.49%), Postives = 326/461 (70.72%), Query Frame = 1

Query: 1   MYGPIFKLRLGTKLCVVLTSPSSVNEVLRHQETVFDNRDATVSALLATYGGVDIVFSQNE 60
           +YGPIFKL LG KLCVV++SPS V +V+R Q+T F +RD  ++ L+AT+GG DI +S   
Sbjct: 73  VYGPIFKLWLGNKLCVVVSSPSLVKQVVRDQDTTFADRDPPIAGLVATFGGNDIAWSNYG 132

Query: 61  SIWKKLRKIFARKMLSKSNLDASYPLRRREVRKIIKGVLES---AGTPIDISKLSLMAAV 120
             W KLRK F  KM+S ++LDA Y LR++EV+  I+ +  +    G PIDI +LS+   V
Sbjct: 133 PEWSKLRKFFVGKMMSNASLDACYALRKQEVKNTIRDLYNNDNKIGKPIDIGELSISTLV 192

Query: 121 KSVMAMTWGGSRGLIGVDGADLEAKFMEVVNELMVLLGTPNLSDLFPVLGGLDLQGIGRK 180
             +  + WG +  +      +L A+    + ELMVL+GTPN+SD+FPVL   D+QGI RK
Sbjct: 193 CVIQNILWGEALEIREEGITNLGAELKFKLAELMVLMGTPNISDVFPVLSWFDIQGIERK 252

Query: 181 MKKVMNVFDGIFNSAIEEQR---------KRGG---DGMENRGFLQLLLEVMEGEDSSES 240
            KK+   F+ I NS +E+ R         +R G   +G  N+ FLQLLLE+ E ED S S
Sbjct: 253 AKKISLWFENIINSTVEKYRSKDFIVEGKERAGKKFEGSRNKNFLQLLLELQENEDGSSS 312

Query: 241 ITDKELKALLVDIIIGGTDTTSTTIEWTIAELIQQPNIMKKVKEELTKVVGLNQMVEEFH 300
           I+  + K +LVDII GGTDTT+T +EWT+AEL+Q P +MKKV+EEL +VVG++  VEEFH
Sbjct: 313 ISMNKFKGVLVDIITGGTDTTTTMVEWTMAELMQHPQVMKKVQEELAQVVGMDSCVEEFH 372

Query: 301 LSKLHYLDAAIKETLRLHPPVPLLVPRTTNQRTTLEGYTIPKSSTIYFNIWAIQRDPKIW 360
           L KL YLDA +KET RLHP +PLLVPR  ++  ++ GYTIPK +T+  N+WAI RDP++W
Sbjct: 373 LPKLKYLDAVVKETFRLHPALPLLVPRRASESGSIGGYTIPKDTTLMLNVWAIHRDPQLW 432

Query: 361 DNPLDFMPERFLNESNENMYDFTGNKIEFCPFGAGKKLCVGIPLAERLLVLILASLLHGF 420
           DNPL+F PERFLN   E+ +D++GN  ++ PFG+G+++C GIPLAER+L+ +LASLLH F
Sbjct: 433 DNPLEFRPERFLNVGVESKFDYSGNNFQYLPFGSGRRMCAGIPLAERMLMFVLASLLHSF 492

Query: 421 EWKLPEGSTLDLEEKFGIVSKKLNPLVVIPTPRLSNLELYN 447
           EW+LP G+ L+L E FGIV KK  PL+ IPTPRLSN ELY+
Sbjct: 493 EWELPAGTKLNLSENFGIVIKKKEPLIAIPTPRLSNSELYH 533

BLAST of Cucsa.302220 vs. TrEMBL
Match: V4U067_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10014858mg PE=3 SV=1)

HSP 1 Score: 484.6 bits (1246), Expect = 1.3e-133
Identity = 245/463 (52.92%), Postives = 328/463 (70.84%), Query Frame = 1

Query: 1   MYGPIFKLRLGTKLCVVLTSPSSVNEVLRHQETVFDNRDATVSALLATYGGVDIVFSQNE 60
           +YGPIFKL LG KLCVV++SPS V +V+R Q+T F  RD  ++ L+AT+GG DI +S   
Sbjct: 73  VYGPIFKLWLGNKLCVVVSSPSLVKQVVRDQDTTFAYRDPPIAGLVATFGGNDIAWSNYG 132

Query: 61  SIWKKLRKIFARKMLSKSNLDASYPLRRREVRKIIKGVLESAGT---PIDISKLSLMAAV 120
             W+KLRK F  KM+S ++LDA Y LR++EV+  I+ +  +      PIDI +LS+   V
Sbjct: 133 PEWRKLRKFFVGKMMSNASLDACYALRKQEVKNTIRDLYNNNNKICKPIDIGELSISTLV 192

Query: 121 KSVMAMTWGGSRGLI--GVDGADLEAKFMEVVNELMVLLGTPNLSDLFPVLGGLDLQGIG 180
            ++  M WG +  L   G     +E KF   + ELMVLLGTPN+SD+FP+L  LD+QGI 
Sbjct: 193 CAMQNMLWGEALELKEKGTTILGVELKFK--LAELMVLLGTPNISDIFPMLSWLDIQGIE 252

Query: 181 RKMKKVMNVFDGIFNSAIEEQR---------KRGGDGME---NRGFLQLLLEVMEGEDSS 240
           R+ KK+   F+ I NS +E+ R         +R G+  E   N+ FLQLLLE+ E ED S
Sbjct: 253 RRAKKISLWFENIINSTVEKYRSKDFNVEGKERAGENFEGSRNKNFLQLLLELQENEDGS 312

Query: 241 ESITDKELKALLVDIIIGGTDTTSTTIEWTIAELIQQPNIMKKVKEELTKVVGLNQMVEE 300
            SI+  + K +L DII GGTDTT+T +EWT+AEL+Q P +MKKV+EEL +VVG++  VEE
Sbjct: 313 SSISMNQFKGVLADIITGGTDTTTTMVEWTMAELMQHPQVMKKVQEELAQVVGMDSCVEE 372

Query: 301 FHLSKLHYLDAAIKETLRLHPPVPLLVPRTTNQRTTLEGYTIPKSSTIYFNIWAIQRDPK 360
           FHL KL YLDA +KE+ RLHP +PLLVPR  ++  ++ GYTIPK +T+  N+WAI RDP+
Sbjct: 373 FHLPKLKYLDAVVKESFRLHPALPLLVPRRASESGSIGGYTIPKDTTLMLNVWAIHRDPQ 432

Query: 361 IWDNPLDFMPERFLNESNENMYDFTGNKIEFCPFGAGKKLCVGIPLAERLLVLILASLLH 420
           +WDNPL+F PERFLN   E+ +D++GN  ++ PFG+G+++C GIPLAER+L+ +LASLLH
Sbjct: 433 LWDNPLEFRPERFLNVGVESKFDYSGNNFQYLPFGSGRRMCAGIPLAERMLMFVLASLLH 492

Query: 421 GFEWKLPEGSTLDLEEKFGIVSKKLNPLVVIPTPRLSNLELYN 447
            FEW+LP G+ L+L EKFGIV KK  PLV IPTPRLSN ELY+
Sbjct: 493 SFEWELPAGTKLNLSEKFGIVIKKKEPLVAIPTPRLSNSELYH 533

BLAST of Cucsa.302220 vs. TrEMBL
Match: A0A067FBZ9_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g009495mg PE=3 SV=1)

HSP 1 Score: 484.6 bits (1246), Expect = 1.3e-133
Identity = 245/463 (52.92%), Postives = 328/463 (70.84%), Query Frame = 1

Query: 1   MYGPIFKLRLGTKLCVVLTSPSSVNEVLRHQETVFDNRDATVSALLATYGGVDIVFSQNE 60
           +YGPIFKL LG KLCVV++SPS V +V+R Q+T F  RD  ++ L+AT+GG DI +S   
Sbjct: 73  VYGPIFKLWLGNKLCVVVSSPSLVKQVVRDQDTTFAYRDPPIAGLVATFGGNDIAWSNYG 132

Query: 61  SIWKKLRKIFARKMLSKSNLDASYPLRRREVRKIIKGVLESAGT---PIDISKLSLMAAV 120
             W+KLRK F  KM+S ++LDA Y LR++EV+  I+ +  +      PIDI +LS+   V
Sbjct: 133 PEWRKLRKFFVGKMMSNASLDACYALRKQEVKNTIRDLYNNNNKICKPIDIGELSISTLV 192

Query: 121 KSVMAMTWGGSRGLI--GVDGADLEAKFMEVVNELMVLLGTPNLSDLFPVLGGLDLQGIG 180
            ++  M WG +  L   G     +E KF   + ELMVLLGTPN+SD+FP+L  LD+QGI 
Sbjct: 193 CAMQNMLWGEALELKEKGTTILGVELKFK--LAELMVLLGTPNISDIFPMLSWLDIQGIE 252

Query: 181 RKMKKVMNVFDGIFNSAIEEQR---------KRGGDGME---NRGFLQLLLEVMEGEDSS 240
           R+ KK+   F+ I NS +E+ R         +R G+  E   N+ FLQLLLE+ E ED S
Sbjct: 253 RRAKKISLWFENIINSTVEKYRSKDFNVEGKERAGENFEGSRNKNFLQLLLELQENEDGS 312

Query: 241 ESITDKELKALLVDIIIGGTDTTSTTIEWTIAELIQQPNIMKKVKEELTKVVGLNQMVEE 300
            SI+  + K +L DII GGTDTT+T +EWT+AEL+Q P +MKKV+EEL +VVG++  VEE
Sbjct: 313 SSISMNQFKGVLADIITGGTDTTTTMVEWTMAELMQHPQVMKKVQEELAQVVGMDSCVEE 372

Query: 301 FHLSKLHYLDAAIKETLRLHPPVPLLVPRTTNQRTTLEGYTIPKSSTIYFNIWAIQRDPK 360
           FHL KL YLDA +KE+ RLHP +PLLVPR  ++  ++ GYTIPK +T+  N+WAI RDP+
Sbjct: 373 FHLPKLKYLDAVVKESFRLHPALPLLVPRRASESGSIGGYTIPKDTTLMLNVWAIHRDPQ 432

Query: 361 IWDNPLDFMPERFLNESNENMYDFTGNKIEFCPFGAGKKLCVGIPLAERLLVLILASLLH 420
           +WDNPL+F PERFLN   E+ +D++GN  ++ PFG+G+++C GIPLAER+L+ +LASLLH
Sbjct: 433 LWDNPLEFRPERFLNVGVESKFDYSGNNFQYLPFGSGRRMCAGIPLAERMLMFVLASLLH 492

Query: 421 GFEWKLPEGSTLDLEEKFGIVSKKLNPLVVIPTPRLSNLELYN 447
            FEW+LP G+ L+L EKFGIV KK  PLV IPTPRLSN ELY+
Sbjct: 493 SFEWELPAGTKLNLSEKFGIVIKKKEPLVAIPTPRLSNSELYH 533

BLAST of Cucsa.302220 vs. TAIR10
Match: AT4G12320.1 (AT4G12320.1 cytochrome P450, family 706, subfamily A, polypeptide 6)

HSP 1 Score: 397.5 bits (1020), Expect = 1.1e-110
Identity = 201/453 (44.37%), Postives = 303/453 (66.89%), Query Frame = 1

Query: 2   YGPIFKLRLGTKLCVVLTSPSSVNEVLRHQETVFDNRDATVSALLATYGGVDIVFSQNES 61
           YGPIFKL LG+KL VV+ +PS   E+L+ Q+  F N D  ++A   TYGG+D+V+    +
Sbjct: 73  YGPIFKLNLGSKLTVVVNTPSLAREILKDQDINFSNHDVPLTARAVTYGGLDLVWLPYGA 132

Query: 62  IWKKLRKIFARKMLSKSNLDASYPLRRREVRKIIKGVLESAG--TPIDISKLSLMAAVKS 121
            W+ LRK+   K+LS   L++ Y LRR+E+R+  + + +     +P+++ +   +  +  
Sbjct: 133 EWRMLRKVCVLKLLSHRTLNSFYELRRKEIRERTRYLYQKGQEESPVNVGEQVFLTMMNL 192

Query: 122 VMAMTWGGSRGLIGVDGADLEA---KFMEVVNELMVLLGTPNLSDLFPVLGGLDLQGIGR 181
            M M WGGS     V   ++E+   +F EV++E+  LLG PN+SD FP L   DLQG+ +
Sbjct: 193 TMNMLWGGS-----VKAEEMESVGTEFKEVISEITRLLGEPNVSDFFPRLARFDLQGLVK 252

Query: 182 KMKKVMNVFDGIFNSAIEEQ---RKRGGDGMENRGFLQLLLEVMEGE-DSSESITDKELK 241
           KM       D I + AIE+    R R GD  E + FLQ L+++ + E DS   IT   +K
Sbjct: 253 KMHVCARELDAILDRAIEQMQLLRTRDGDDGECKDFLQHLMKLKDQEADSEVPITVNHVK 312

Query: 242 ALLVDIIIGGTDTTSTTIEWTIAELIQQPNIMKKVKEELTKVVGLNQMVEEFHLSKLHYL 301
           A+LVD+++GGTDT++ TIE+ +AELI++P +MK+ ++EL +VVG + ++EE H+++L ++
Sbjct: 313 AVLVDLVVGGTDTSTNTIEFAMAELIRKPELMKRAQQELDEVVGKDNIIEESHITRLPFI 372

Query: 302 DAAIKETLRLHPPVPLLVPRTTNQRTTLEGYTIPKSSTIYFNIWAIQRDPKIWDNPLDFM 361
            A +KETLRL+P +PLLVP   ++   + GYTIPK++ I+ N+W+IQRDP +W+ P +F 
Sbjct: 373 SAIMKETLRLYPTIPLLVPHRPSETALVGGYTIPKNTKIFINVWSIQRDPNVWEYPTEFR 432

Query: 362 PERFLNESNENMYDFTGNKIEFCPFGAGKKLCVGIPLAERLLVLILASLLHGFEWKLPEG 421
           PERFL++ +    DFTG    + PFG+G+++C GI LAER+++  LA+LLH F+WK+PEG
Sbjct: 433 PERFLDKKS---CDFTGTDYSYLPFGSGRRICAGIALAERMILYTLATLLHSFDWKIPEG 492

Query: 422 STLDLEEKFGIVSKKLNPLVVIPTPRLSNLELY 446
             LDL+EKFGIV K  +PLV +P PRLSN  LY
Sbjct: 493 HILDLKEKFGIVLKLKSPLVALPVPRLSNSNLY 517

BLAST of Cucsa.302220 vs. TAIR10
Match: AT4G12300.1 (AT4G12300.1 cytochrome P450, family 706, subfamily A, polypeptide 4)

HSP 1 Score: 396.0 bits (1016), Expect = 3.2e-110
Identity = 203/451 (45.01%), Postives = 297/451 (65.85%), Query Frame = 1

Query: 2   YGPIFKLRLGTKLCVVLTSPSSVNEVLRHQETVFDNRDATVSALLATYGGVDIVFSQNES 61
           +GPIFKL LG+KL +V+ SPS   E+L+ Q+  F NRD  ++   ATYGG+DIV++   +
Sbjct: 73  HGPIFKLNLGSKLTIVVNSPSLAREILKDQDINFSNRDVPLTGRAATYGGIDIVWTPYGA 132

Query: 62  IWKKLRKIFARKMLSKSNLDASYPLRRREVRKIIKGVLESAG--TPIDISKLSLMAAVKS 121
            W++LRKI   K+LS+  LD+ Y LRR+EVR+  + + E     +P+ +     +  +  
Sbjct: 133 EWRQLRKICVLKLLSRKTLDSFYELRRKEVRERTRYLYEQGRKQSPVKVGDQLFLTMMNL 192

Query: 122 VMAMTWGGSRGLIGVDGADLEA---KFMEVVNELMVLLGTPNLSDLFPVLGGLDLQGIGR 181
            M M WGGS     V   ++E+   +F  V++E+  LL  PN+SD FP L   DLQG+ +
Sbjct: 193 TMNMLWGGS-----VKAEEMESVGTEFKGVISEITRLLSEPNVSDFFPWLARFDLQGLVK 252

Query: 182 KMKKVMNVFDGIFNSAIEEQRK-RGGDGMENRGFLQLLLEVMEGEDSSE-SITDKELKAL 241
           +M       D + + AIE+ +  RG D  E + FLQ L+++ + E  SE  IT   +KAL
Sbjct: 253 RMGVCARELDAVLDRAIEQMKPLRGRDDDEVKDFLQYLMKLKDQEGDSEVPITINHVKAL 312

Query: 242 LVDIIIGGTDTTSTTIEWTIAELIQQPNIMKKVKEELTKVVGLNQMVEEFHLSKLHYLDA 301
           L D+++GGTDT++ TIE+ +AEL+  P ++K+ +EEL +VVG + +VEE H+++L Y+ A
Sbjct: 313 LTDMVVGGTDTSTNTIEFAMAELMSNPELIKRAQEELDEVVGKDNIVEESHITRLPYILA 372

Query: 302 AIKETLRLHPPVPLLVPRTTNQRTTLEGYTIPKSSTIYFNIWAIQRDPKIWDNPLDFMPE 361
            +KETLRLHP +PLLVP    + T + GYTIPK + I+ N+W+IQRDP +W+NP +F PE
Sbjct: 373 IMKETLRLHPTLPLLVPHRPAENTVVGGYTIPKDTKIFVNVWSIQRDPNVWENPTEFRPE 432

Query: 362 RFLNESNENMYDFTGNKIEFCPFGAGKKLCVGIPLAERLLVLILASLLHGFEWKLPEGST 421
           RFL   + N  DFTG    + PFG+G+++C G+ LAER+++  LA+LLH F+WK+PEG  
Sbjct: 433 RFL---DNNSCDFTGANYSYFPFGSGRRICAGVALAERMVLYTLATLLHSFDWKIPEGHV 492

Query: 422 LDLEEKFGIVSKKLNPLVVIPTPRLSNLELY 446
           LDL+EKFGIV K   PLV +P PR S+  LY
Sbjct: 493 LDLKEKFGIVLKLKIPLVALPIPRFSDSNLY 515

BLAST of Cucsa.302220 vs. TAIR10
Match: AT4G12310.1 (AT4G12310.1 cytochrome P450, family 706, subfamily A, polypeptide 5)

HSP 1 Score: 392.1 bits (1006), Expect = 4.6e-109
Identity = 198/453 (43.71%), Postives = 300/453 (66.23%), Query Frame = 1

Query: 2   YGPIFKLRLGTKLCVVLTSPSSVNEVLRHQETVFDNRDATVSALLATYGGVDIVFSQNES 61
           +GPIFKL LG+KL VV+ SPS  +E+L+ Q+  F N D  ++A   TYGG+D+V+    +
Sbjct: 75  HGPIFKLNLGSKLTVVVNSPSLASEILKDQDINFSNHDVPLTARAVTYGGLDLVWLPYGA 134

Query: 62  IWKKLRKIFARKMLSKSNLDASYPLRRREVRKIIKGVLESA--GTPIDISKLSLMAAVKS 121
            W+ LRK+ A K+ S+  LD+ Y LRR+E+R+  + + +     +P+++ +   +  +  
Sbjct: 135 EWRMLRKVCAAKLFSRKTLDSFYELRRKEIRERTRCLYQKGLEKSPVNVGEQLFLTMMNL 194

Query: 122 VMAMTWGGSRGLIGVDGADLEA---KFMEVVNELMVLLGTPNLSDLFPVLGGLDLQGIGR 181
           +M M WGGS     V   D+E+   +F  V++E+  LLG PN+SD FP+L   DLQG+ +
Sbjct: 195 MMNMLWGGS-----VKAEDMESVGTEFKGVISEITRLLGVPNVSDFFPMLARFDLQGLVK 254

Query: 182 KMKKVMNVFDGIFNSAIEEQ---RKRGGDGMENRGFLQLLLEVMEGE-DSSESITDKELK 241
           KM       D I + AIE+    R R GD  E + FLQ L+++ + E DS   IT   +K
Sbjct: 255 KMHLYARDLDAILDRAIEQMQRLRSRDGDDGECKDFLQHLMKLRDQEADSDVPITMNHVK 314

Query: 242 ALLVDIIIGGTDTTSTTIEWTIAELIQQPNIMKKVKEELTKVVGLNQMVEEFHLSKLHYL 301
           A+L+D+++GGT++++ TIE+ +AELI  P +M++ ++EL +VVG + +VEE H++ L Y+
Sbjct: 315 AVLMDMVVGGTESSTNTIEFVMAELISNPELMRRAQQELDEVVGKDNIVEESHITSLPYI 374

Query: 302 DAAIKETLRLHPPVPLLVPRTTNQRTTLEGYTIPKSSTIYFNIWAIQRDPKIWDNPLDFM 361
            A +KETLRL+P +PLLVP   ++   + GYTIPK++ I+ N+W+IQRDP +W+ P +F 
Sbjct: 375 LAVLKETLRLYPTIPLLVPHRPSETALVGGYTIPKNTKIFINVWSIQRDPNVWEYPTEFR 434

Query: 362 PERFLNESNENMYDFTGNKIEFCPFGAGKKLCVGIPLAERLLVLILASLLHGFEWKLPEG 421
           PERFL++ +    DFTG    + PFG+G+++C GI LAER+++  LA+LLH F+W +P+G
Sbjct: 435 PERFLDKKS---CDFTGTDYSYLPFGSGRRICAGIALAERMILYTLATLLHSFDWTIPDG 494

Query: 422 STLDLEEKFGIVSKKLNPLVVIPTPRLSNLELY 446
             LDLEEKFGIV K   PLV +P PRLSN   Y
Sbjct: 495 HVLDLEEKFGIVLKLKTPLVALPIPRLSNSNFY 519

BLAST of Cucsa.302220 vs. TAIR10
Match: AT5G44620.1 (AT5G44620.1 cytochrome P450, family 706, subfamily A, polypeptide 3)

HSP 1 Score: 369.0 bits (946), Expect = 4.1e-102
Identity = 189/453 (41.72%), Postives = 289/453 (63.80%), Query Frame = 1

Query: 2   YGPIFKLRLGTKLCVVLTSPSSVNEVLRHQETVFDNRDATVSALLATYGGVDIVFSQNES 61
           +GPIFKL LG KL +V+TS     E+L+  + +F N D      + TYGG +I++S    
Sbjct: 77  HGPIFKLWLGAKLTIVVTSSEVAQEILKTNDIIFANHDVPAVGPVNTYGGTEIIWSPYGP 136

Query: 62  IWKKLRKIFARKMLSKSNLDASYPLRRREVRKIIKGVLESA--GTPIDISKLSLMAAVKS 121
            W+ LRK+   ++L  + LD+S  LRRRE R+ ++ + + A  G+P+++ +   +  +  
Sbjct: 137 KWRMLRKLCVNRILRNAMLDSSTDLRRRETRQTVRYLADQARVGSPVNLGEQIFLMMLNV 196

Query: 122 VMAMTWGGS-----RGLIGVDGADLEAKFMEVVNELMVLLGTPNLSDLFPVLGGLDLQGI 181
           V  M WG +     R ++G       A+F+EV+ E+  LL  PN+SD FPVL   DLQG+
Sbjct: 197 VTQMLWGTTVKEEEREVVG-------AEFLEVIREMNDLLLVPNISDFFPVLSRFDLQGL 256

Query: 182 GRKMKKVMNVFDGIFNSAIEEQRKRGGDGMENRG--FLQLLLEVMEGEDSSESITDKELK 241
            ++M++     D +F+  I ++     D  + R   FL +LL+V + E     +T  ++K
Sbjct: 257 AKRMRRPAQRMDQMFDRIINQRLGMDRDSSDGRAVDFLDVLLKVKDEEAEKTKLTMNDVK 316

Query: 242 ALLVDIIIGGTDTTSTTIEWTIAELIQQPNIMKKVKEELTKVVGLNQMVEEFHLSKLHYL 301
           A+L+D+++GGTDT+   IE+ +AEL+  P+IMK+ ++E+ KVVG  ++VEE H+SKL Y+
Sbjct: 317 AVLMDMVLGGTDTSLHVIEFAMAELLHNPDIMKRAQQEVDKVVGKEKVVEESHISKLPYI 376

Query: 302 DAAIKETLRLHPPVPLLVPRTTNQRTTLEGYTIPKSSTIYFNIWAIQRDPKIWDNPLDFM 361
            A +KETLRLH   PLLVPR  +Q T + G+TIPK S I+ N WAI R+P +W+NPL F 
Sbjct: 377 LAIMKETLRLHTVAPLLVPRRPSQTTVVGGFTIPKDSKIFINAWAIHRNPNVWENPLKFD 436

Query: 362 PERFLNESNENMYDFTGNKIEFCPFGAGKKLCVGIPLAERLLVLILASLLHGFEWKLPEG 421
           P+RFL+ S    YDF GN   + PFG+G+++CVG+ + ER+++  LA+ LH F+WK+P+G
Sbjct: 437 PDRFLDMS----YDFKGNDFNYLPFGSGRRICVGMAMGERVVLYNLATFLHSFDWKIPQG 496

Query: 422 STLDLEEKFGIVSKKLNPLVVIPTPRLSNLELY 446
             +++EEKFGIV +  NPLV  P  RLS+  LY
Sbjct: 497 ERVEVEEKFGIVLELKNPLVATPVLRLSDPNLY 518

BLAST of Cucsa.302220 vs. TAIR10
Match: AT4G12330.1 (AT4G12330.1 cytochrome P450, family 706, subfamily A, polypeptide 7)

HSP 1 Score: 366.7 bits (940), Expect = 2.0e-101
Identity = 185/451 (41.02%), Postives = 294/451 (65.19%), Query Frame = 1

Query: 2   YGPIFKLRLGTKLCVVLTSPSSVNEVLRHQETVFDNRDATVSALLATYGGVDIVFSQNES 61
           +GP+FKL LG KL +V+TS  +  ++LR  + +F N D  V+  L+TYGGVDIV+S    
Sbjct: 77  HGPVFKLWLGAKLTIVITSSEATRDILRTNDVIFANDDVPVAGSLSTYGGVDIVWSPYGP 136

Query: 62  IWKKLRKIFARKMLSKSNLDASY--PLRRREVRKIIKGVLESA--GTPIDISKLSLMAAV 121
            W  LRKI   KMLS + LD++    LRR+E R+ ++ + + A  G  +++ +   +  +
Sbjct: 137 EWPMLRKICINKMLSNATLDSNSFSALRRQETRRTVRYLADRARAGLAVNVGEQIFVTIL 196

Query: 122 KSVMAMTWGGSRGLIGVDGADLE---AKFMEVVNELMVLLGTPNLSDLFPVLGGLDLQGI 181
             V  M WG +      D  + E   A+F+E++ E++ ++G PN+SD FPVL   DLQG+
Sbjct: 197 NVVTQMLWGETVA----DDEEREKVGAEFLELITEIIDVVGKPNVSDFFPVLSRFDLQGL 256

Query: 182 GRKMKKVMNVFDGIFNSAIEEQRKRGGDGMENRGFLQLLLEVMEGEDSSESITDKELKAL 241
            +++++     D +F+  I ++         N G    L+ ++  +D  E+++   +KAL
Sbjct: 257 AKRVRRSAQRMDRMFDRIISQRMGMDKGSKGNGG--DFLMVLLNAKDEDENMSMNHVKAL 316

Query: 242 LVDIIIGGTDTTSTTIEWTIAELIQQPNIMKKVKEELTKVVGLNQMVEEFHLSKLHYLDA 301
           L+D+++GGTDT+  TIE+ +AELI +  IMK+ ++EL KVVG N +VEE H++KL Y+ +
Sbjct: 317 LMDMVLGGTDTSLNTIEFAMAELINKLEIMKRAQQELDKVVGKNNIVEEKHITKLPYILS 376

Query: 302 AIKETLRLHPPVPLLVPRTTNQRTTLEGYTIPKSSTIYFNIWAIQRDPKIWDNPLDFMPE 361
            +KETLRLHP +PLL+PR  ++ T + GYTIP  S ++ N+WAI R+P +W+NPL+F P+
Sbjct: 377 IMKETLRLHPALPLLIPRCPSETTVIGGYTIPNDSKVFINVWAIHRNPNVWENPLEFNPD 436

Query: 362 RFLNESNENMYDFTGNKIEFCPFGAGKKLCVGIPLAERLLVLILASLLHGFEWKLPEGST 421
           RFL++     YDF+GN   + PFG+G+++C G+ +AE++++  LA+LLH F+W++ EG  
Sbjct: 437 RFLDKG----YDFSGNDYSYFPFGSGRRICAGMAMAEKVVLYNLATLLHSFDWRIGEGEK 496

Query: 422 LDLEEKFGIVSKKLNPLVVIPTPRLSNLELY 446
           ++LEEKFGI+ K  NPLV  P  RLS+  L+
Sbjct: 497 VELEEKFGILLKLKNPLVATPVLRLSDPNLF 517

BLAST of Cucsa.302220 vs. NCBI nr
Match: gi|778699919|ref|XP_004147610.2| (PREDICTED: geraniol 8-hydroxylase-like [Cucumis sativus])

HSP 1 Score: 884.0 bits (2283), Expect = 1.1e-253
Identity = 448/448 (100.00%), Postives = 448/448 (100.00%), Query Frame = 1

Query: 1   MYGPIFKLRLGTKLCVVLTSPSSVNEVLRHQETVFDNRDATVSALLATYGGVDIVFSQNE 60
           MYGPIFKLRLGTKLCVVLTSPSSVNEVLRHQETVFDNRDATVSALLATYGGVDIVFSQNE
Sbjct: 79  MYGPIFKLRLGTKLCVVLTSPSSVNEVLRHQETVFDNRDATVSALLATYGGVDIVFSQNE 138

Query: 61  SIWKKLRKIFARKMLSKSNLDASYPLRRREVRKIIKGVLESAGTPIDISKLSLMAAVKSV 120
           SIWKKLRKIFARKMLSKSNLDASYPLRRREVRKIIKGVLESAGTPIDISKLSLMAAVKSV
Sbjct: 139 SIWKKLRKIFARKMLSKSNLDASYPLRRREVRKIIKGVLESAGTPIDISKLSLMAAVKSV 198

Query: 121 MAMTWGGSRGLIGVDGADLEAKFMEVVNELMVLLGTPNLSDLFPVLGGLDLQGIGRKMKK 180
           MAMTWGGSRGLIGVDGADLEAKFMEVVNELMVLLGTPNLSDLFPVLGGLDLQGIGRKMKK
Sbjct: 199 MAMTWGGSRGLIGVDGADLEAKFMEVVNELMVLLGTPNLSDLFPVLGGLDLQGIGRKMKK 258

Query: 181 VMNVFDGIFNSAIEEQRKRGGDGMENRGFLQLLLEVMEGEDSSESITDKELKALLVDIII 240
           VMNVFDGIFNSAIEEQRKRGGDGMENRGFLQLLLEVMEGEDSSESITDKELKALLVDIII
Sbjct: 259 VMNVFDGIFNSAIEEQRKRGGDGMENRGFLQLLLEVMEGEDSSESITDKELKALLVDIII 318

Query: 241 GGTDTTSTTIEWTIAELIQQPNIMKKVKEELTKVVGLNQMVEEFHLSKLHYLDAAIKETL 300
           GGTDTTSTTIEWTIAELIQQPNIMKKVKEELTKVVGLNQMVEEFHLSKLHYLDAAIKETL
Sbjct: 319 GGTDTTSTTIEWTIAELIQQPNIMKKVKEELTKVVGLNQMVEEFHLSKLHYLDAAIKETL 378

Query: 301 RLHPPVPLLVPRTTNQRTTLEGYTIPKSSTIYFNIWAIQRDPKIWDNPLDFMPERFLNES 360
           RLHPPVPLLVPRTTNQRTTLEGYTIPKSSTIYFNIWAIQRDPKIWDNPLDFMPERFLNES
Sbjct: 379 RLHPPVPLLVPRTTNQRTTLEGYTIPKSSTIYFNIWAIQRDPKIWDNPLDFMPERFLNES 438

Query: 361 NENMYDFTGNKIEFCPFGAGKKLCVGIPLAERLLVLILASLLHGFEWKLPEGSTLDLEEK 420
           NENMYDFTGNKIEFCPFGAGKKLCVGIPLAERLLVLILASLLHGFEWKLPEGSTLDLEEK
Sbjct: 439 NENMYDFTGNKIEFCPFGAGKKLCVGIPLAERLLVLILASLLHGFEWKLPEGSTLDLEEK 498

Query: 421 FGIVSKKLNPLVVIPTPRLSNLELYNMM 449
           FGIVSKKLNPLVVIPTPRLSNLELYNMM
Sbjct: 499 FGIVSKKLNPLVVIPTPRLSNLELYNMM 526

BLAST of Cucsa.302220 vs. NCBI nr
Match: gi|659073586|ref|XP_008437142.1| (PREDICTED: flavonoid 3'-monooxygenase-like [Cucumis melo])

HSP 1 Score: 752.3 bits (1941), Expect = 4.9e-214
Identity = 376/448 (83.93%), Postives = 404/448 (90.18%), Query Frame = 1

Query: 1   MYGPIFKLRLGTKLCVVLTSPSSVNEVLRHQETVFDNRDATVSALLATYGGVDIVFSQNE 60
           +YGPIFKLRLG KLC VLTSPSS+NEVLRH ETVFDNRDATVSA L TYGG DIVFSQNE
Sbjct: 74  IYGPIFKLRLGAKLCFVLTSPSSINEVLRHHETVFDNRDATVSARLCTYGGSDIVFSQNE 133

Query: 61  SIWKKLRKIFARKMLSKSNLDASYPLRRREVRKIIKGVLESAGTPIDISKLSLMAAVKSV 120
             WKKLRKIFARKMLSK+NLDASYPLRR+EVRKIIKGVLESAGTPID  +LS +AAVKSV
Sbjct: 134 GDWKKLRKIFARKMLSKANLDASYPLRRQEVRKIIKGVLESAGTPIDFGELSFLAAVKSV 193

Query: 121 MAMTWGGSRGLIGVDGADLEAKFMEVVNELMVLLGTPNLSDLFPVLGGLDLQGIGRKMKK 180
           MAM WGGS GL GV   DLEAKF EVV+ELMVLLGTPNLSDLFPVLG LDLQGI RKMKK
Sbjct: 194 MAMIWGGSGGLTGVAVTDLEAKFREVVDELMVLLGTPNLSDLFPVLGCLDLQGIARKMKK 253

Query: 181 VMNVFDGIFNSAIEEQRKRGGDGMENRGFLQLLLEVMEGEDSSESITDKELKALLVDIII 240
           V N  D I NSAIE QRK+G +G+ENRGFL+ LLEVMEGED+SESIT+ +LK LL+DI+I
Sbjct: 254 VSNRCDEILNSAIEGQRKKGRNGLENRGFLEFLLEVMEGEDNSESITENQLKGLLIDIVI 313

Query: 241 GGTDTTSTTIEWTIAELIQQPNIMKKVKEELTKVVGLNQMVEEFHLSKLHYLDAAIKETL 300
           GGTDTTST IEWT+ EL+QQPN MKKV EELT VVGLNQMVEEFHLSKLHYLDAAIKETL
Sbjct: 314 GGTDTTSTVIEWTMTELLQQPNTMKKVIEELTNVVGLNQMVEEFHLSKLHYLDAAIKETL 373

Query: 301 RLHPPVPLLVPRTTNQRTTLEGYTIPKSSTIYFNIWAIQRDPKIWDNPLDFMPERFLNES 360
           RLHPPV LLVPR +++ TTLEGYTIPK STIYFN+WAIQRDPK+W+NPLDFMPERFLNES
Sbjct: 374 RLHPPVSLLVPRVSSKTTTLEGYTIPKGSTIYFNVWAIQRDPKVWENPLDFMPERFLNES 433

Query: 361 NENMYDFTGNKIEFCPFGAGKKLCVGIPLAERLLVLILASLLHGFEWKLPEGSTLDLEEK 420
           NE MYDF+GNKIEFCPFG+GKK C GIPLAERLLVLILASLLHGFEWKLPEGS LDLEEK
Sbjct: 434 NEKMYDFSGNKIEFCPFGSGKKSCAGIPLAERLLVLILASLLHGFEWKLPEGSKLDLEEK 493

Query: 421 FGIVSKKLNPLVVIPTPRLSNLELYNMM 449
           FGIV++KLNPLVVIPTPRLSNLELYN M
Sbjct: 494 FGIVTRKLNPLVVIPTPRLSNLELYNTM 521

BLAST of Cucsa.302220 vs. NCBI nr
Match: gi|659073582|ref|XP_008437139.1| (PREDICTED: 7-ethoxycoumarin O-deethylase-like isoform X1 [Cucumis melo])

HSP 1 Score: 733.4 bits (1892), Expect = 2.4e-208
Identity = 361/448 (80.58%), Postives = 401/448 (89.51%), Query Frame = 1

Query: 1   MYGPIFKLRLGTKLCVVLTSPSSVNEVLRHQETVFDNRDATVSALLATYGGVDIVFSQNE 60
           +YG I KL LGTKLC+VLTSPSSVNE LRHQET+F  RD TV ALL++YGG DIVF+Q+E
Sbjct: 79  VYGSILKLHLGTKLCIVLTSPSSVNEALRHQETIFSIRDTTVVALLSSYGGSDIVFTQDE 138

Query: 61  SIWKKLRKIFARKMLSKSNLDASYPLRRREVRKIIKGVLESAGTPIDISKLSLMAAVKSV 120
           + WKKLRKIF RKMLSKSNLDASY LRR+EVRK+IKGV ESAGTPIDI KL  +AA+KSV
Sbjct: 139 NNWKKLRKIFTRKMLSKSNLDASYSLRRQEVRKVIKGVFESAGTPIDIGKLGFVAALKSV 198

Query: 121 MAMTWGGSRGLIGVDGADLEAKFMEVVNELMVLLGTPNLSDLFPVLGGLDLQGIGRKMKK 180
           MAMTWGGS GLIGVDG DL+AKF E V+E+MVLL TPNLSDLFPVLG  DLQG+ RKMKK
Sbjct: 199 MAMTWGGSGGLIGVDGTDLDAKFREAVDEMMVLLATPNLSDLFPVLGRFDLQGVVRKMKK 258

Query: 181 VMNVFDGIFNSAIEEQRKRGGDGMENRGFLQLLLEVMEGEDSSESITDKELKALLVDIII 240
           VM VFD IFNSAIEEQRKRGG+GMENRGFLQ LLE+MEGEDSSESIT+  LKALL+DI+I
Sbjct: 259 VMCVFDEIFNSAIEEQRKRGGNGMENRGFLQFLLEIMEGEDSSESITENNLKALLMDIVI 318

Query: 241 GGTDTTSTTIEWTIAELIQQPNIMKKVKEELTKVVGLNQMVEEFHLSKLHYLDAAIKETL 300
           GGTDTTSTT+EW I+ELI+QPN M KV EELTKVVGLNQMVEEFHL KL++LDA +KETL
Sbjct: 319 GGTDTTSTTVEWAISELIKQPNTMNKVIEELTKVVGLNQMVEEFHLPKLNFLDAVVKETL 378

Query: 301 RLHPPVPLLVPRTTNQRTTLEGYTIPKSSTIYFNIWAIQRDPKIWDNPLDFMPERFLNES 360
           RLHPP+PLLVPRT++Q TT   YTIPK STIYFNIWAIQRDPK+WDNPL+F PERFLNES
Sbjct: 379 RLHPPLPLLVPRTSSQTTTFGEYTIPKGSTIYFNIWAIQRDPKVWDNPLNFEPERFLNES 438

Query: 361 NENMYDFTGNKIEFCPFGAGKKLCVGIPLAERLLVLILASLLHGFEWKLPEGSTLDLEEK 420
           +EN+YDFTGN+IEFCPFG+GKK C GIPLA+RLLVLILASLLHGFEWKLPEGS  D+EEK
Sbjct: 439 SENLYDFTGNRIEFCPFGSGKKSCAGIPLAKRLLVLILASLLHGFEWKLPEGSKFDMEEK 498

Query: 421 FGIVSKKLNPLVVIPTPRLSNLELYNMM 449
           FGIV+KKLNPLV IPTPRL NLELYN+M
Sbjct: 499 FGIVTKKLNPLVTIPTPRLPNLELYNIM 526

BLAST of Cucsa.302220 vs. NCBI nr
Match: gi|659073584|ref|XP_008437141.1| (PREDICTED: 7-ethoxycoumarin O-deethylase-like isoform X2 [Cucumis melo])

HSP 1 Score: 733.4 bits (1892), Expect = 2.4e-208
Identity = 361/448 (80.58%), Postives = 401/448 (89.51%), Query Frame = 1

Query: 1   MYGPIFKLRLGTKLCVVLTSPSSVNEVLRHQETVFDNRDATVSALLATYGGVDIVFSQNE 60
           +YG I KL LGTKLC+VLTSPSSVNE LRHQET+F  RD TV ALL++YGG DIVF+Q+E
Sbjct: 73  VYGSILKLHLGTKLCIVLTSPSSVNEALRHQETIFSIRDTTVVALLSSYGGSDIVFTQDE 132

Query: 61  SIWKKLRKIFARKMLSKSNLDASYPLRRREVRKIIKGVLESAGTPIDISKLSLMAAVKSV 120
           + WKKLRKIF RKMLSKSNLDASY LRR+EVRK+IKGV ESAGTPIDI KL  +AA+KSV
Sbjct: 133 NNWKKLRKIFTRKMLSKSNLDASYSLRRQEVRKVIKGVFESAGTPIDIGKLGFVAALKSV 192

Query: 121 MAMTWGGSRGLIGVDGADLEAKFMEVVNELMVLLGTPNLSDLFPVLGGLDLQGIGRKMKK 180
           MAMTWGGS GLIGVDG DL+AKF E V+E+MVLL TPNLSDLFPVLG  DLQG+ RKMKK
Sbjct: 193 MAMTWGGSGGLIGVDGTDLDAKFREAVDEMMVLLATPNLSDLFPVLGRFDLQGVVRKMKK 252

Query: 181 VMNVFDGIFNSAIEEQRKRGGDGMENRGFLQLLLEVMEGEDSSESITDKELKALLVDIII 240
           VM VFD IFNSAIEEQRKRGG+GMENRGFLQ LLE+MEGEDSSESIT+  LKALL+DI+I
Sbjct: 253 VMCVFDEIFNSAIEEQRKRGGNGMENRGFLQFLLEIMEGEDSSESITENNLKALLMDIVI 312

Query: 241 GGTDTTSTTIEWTIAELIQQPNIMKKVKEELTKVVGLNQMVEEFHLSKLHYLDAAIKETL 300
           GGTDTTSTT+EW I+ELI+QPN M KV EELTKVVGLNQMVEEFHL KL++LDA +KETL
Sbjct: 313 GGTDTTSTTVEWAISELIKQPNTMNKVIEELTKVVGLNQMVEEFHLPKLNFLDAVVKETL 372

Query: 301 RLHPPVPLLVPRTTNQRTTLEGYTIPKSSTIYFNIWAIQRDPKIWDNPLDFMPERFLNES 360
           RLHPP+PLLVPRT++Q TT   YTIPK STIYFNIWAIQRDPK+WDNPL+F PERFLNES
Sbjct: 373 RLHPPLPLLVPRTSSQTTTFGEYTIPKGSTIYFNIWAIQRDPKVWDNPLNFEPERFLNES 432

Query: 361 NENMYDFTGNKIEFCPFGAGKKLCVGIPLAERLLVLILASLLHGFEWKLPEGSTLDLEEK 420
           +EN+YDFTGN+IEFCPFG+GKK C GIPLA+RLLVLILASLLHGFEWKLPEGS  D+EEK
Sbjct: 433 SENLYDFTGNRIEFCPFGSGKKSCAGIPLAKRLLVLILASLLHGFEWKLPEGSKFDMEEK 492

Query: 421 FGIVSKKLNPLVVIPTPRLSNLELYNMM 449
           FGIV+KKLNPLV IPTPRL NLELYN+M
Sbjct: 493 FGIVTKKLNPLVTIPTPRLPNLELYNIM 520

BLAST of Cucsa.302220 vs. NCBI nr
Match: gi|659073592|ref|XP_008437145.1| (PREDICTED: 7-ethoxycoumarin O-deethylase-like [Cucumis melo])

HSP 1 Score: 596.3 bits (1536), Expect = 4.5e-167
Identity = 296/447 (66.22%), Postives = 357/447 (79.87%), Query Frame = 1

Query: 1   MYGPIFKLRLGTKLCVVLTSPSSVNEVLRHQETVFDNRDATVSALLATYGGVDIVFSQNE 60
           +YGP++KLRLG KLC+VL SP+SV E   HQET+F NRD TV ALL+ YGG  IVF+Q+E
Sbjct: 112 IYGPVYKLRLGPKLCIVLNSPASVKEAFHHQETLFPNRDTTVCALLSGYGGSGIVFAQDE 171

Query: 61  SIWKKLRKIFARKMLSKSNLDASYPLRRREVRKIIKGVLESAGTPIDISKLSLMAAVKSV 120
             WKKLRKIF RKM+SKSNLDASY +RR+EVRK+IKGV ES GT IDI K+S  A +KSV
Sbjct: 172 GDWKKLRKIFTRKMISKSNLDASYFVRRQEVRKVIKGVFESVGTQIDIGKVSFHATMKSV 231

Query: 121 MAMTWGGSRGLIGVDGADLEAKFMEVVNELMVLLGTPNLSDLFPVLGGLDLQGIGRKMKK 180
           +AMTWG S  L+G DG  LE KF E+++ELMVLLG+PN+SD+FPVLG  DLQGIGR+ KK
Sbjct: 232 LAMTWGDSGRLMGEDGVGLEEKFREMMDELMVLLGSPNVSDVFPVLGRFDLQGIGRRTKK 291

Query: 181 VMNVFDGIFNSAIEEQRKRG--GDGMENRGFLQLLLEVMEGEDSSESITDKELKALLVDI 240
           VM V DGI N +IEEQRK G  G+G    G+LQLLLE+ + ED+SE IT+ +LKALL+DI
Sbjct: 292 VMGVLDGILNFSIEEQRKMGGKGNGEGKGGYLQLLLELQDNEDNSECITNDQLKALLLDI 351

Query: 241 IIGGTDTTSTTIEWTIAELIQQPNIMKKVKEELTKVVGLNQMVEEFHLSKLHYLDAAIKE 300
           +IGGT+TT+TT+EW +AEL+Q PN +KKVKEEL KV+GLN +VEEFH SKL YL+A IKE
Sbjct: 352 VIGGTETTATTVEWAMAELMQHPNTIKKVKEELKKVIGLNAVVEEFHFSKLCYLNAVIKE 411

Query: 301 TLRLHPPVPLLVPRTTNQRTTLEGYTIPKSSTIYFNIWAIQRDPKIWDNPLDFMPERFLN 360
           TLRLHPP+ LLVPR+    TTL GY IPK ST+YFN+WAIQRDPK WDNP  FMPERF+N
Sbjct: 412 TLRLHPPIFLLVPRSLTSSTTLGGYYIPKDSTLYFNLWAIQRDPKTWDNPSKFMPERFIN 471

Query: 361 ESNENMYDFTGNKIEFCPFGAGKKLCVGIPLAERLLVLILASLLHGFEWKLPEGSTLDLE 420
            +N+       N +EF PFG GKK C G+ LAER+L+ ILASLLH F+W+LP+ S +D +
Sbjct: 472 NNND-------NAMEFYPFGYGKKSCAGVALAERMLMFILASLLHSFDWELPKDSVIDFK 531

Query: 421 EKFGIVSKKLNPLVVIPTPRLSNLELY 446
           EKFGIV+KKLNPLV IPTP LSN +LY
Sbjct: 532 EKFGIVNKKLNPLVAIPTPTLSNSDLY 551

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
F3PH_PETHY1.2e-7936.63Flavonoid 3'-monooxygenase OS=Petunia hybrida GN=CYP75B2 PE=2 SV=1[more]
F3PH_ARATH1.3e-7837.98Flavonoid 3'-monooxygenase OS=Arabidopsis thaliana GN=CYP75B1 PE=1 SV=1[more]
C76BA_SWEMU2.3e-7837.53Geraniol 8-hydroxylase OS=Swertia mussotii GN=CYP76B10 PE=1 SV=1[more]
C76C2_ARATH2.8e-7635.41Cytochrome P450 76C2 OS=Arabidopsis thaliana GN=CYP76C2 PE=2 SV=1[more]
C76B6_CATRO3.7e-7635.81Geraniol 8-hydroxylase OS=Catharanthus roseus GN=CYP76B6 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
M5WDL9_PRUPE7.3e-14053.76Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa019218mg PE=3 SV=1[more]
M5WSD8_PRUPE1.2e-13452.43Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa019849mg PE=3 SV=1[more]
A0A067FFE7_CITSI2.7e-13452.49Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g009481mg PE=3 SV=1[more]
V4U067_9ROSI1.3e-13352.92Uncharacterized protein OS=Citrus clementina GN=CICLE_v10014858mg PE=3 SV=1[more]
A0A067FBZ9_CITSI1.3e-13352.92Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g009495mg PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT4G12320.11.1e-11044.37 cytochrome P450, family 706, subfamily A, polypeptide 6[more]
AT4G12300.13.2e-11045.01 cytochrome P450, family 706, subfamily A, polypeptide 4[more]
AT4G12310.14.6e-10943.71 cytochrome P450, family 706, subfamily A, polypeptide 5[more]
AT5G44620.14.1e-10241.72 cytochrome P450, family 706, subfamily A, polypeptide 3[more]
AT4G12330.12.0e-10141.02 cytochrome P450, family 706, subfamily A, polypeptide 7[more]
Match NameE-valueIdentityDescription
gi|778699919|ref|XP_004147610.2|1.1e-253100.00PREDICTED: geraniol 8-hydroxylase-like [Cucumis sativus][more]
gi|659073586|ref|XP_008437142.1|4.9e-21483.93PREDICTED: flavonoid 3'-monooxygenase-like [Cucumis melo][more]
gi|659073582|ref|XP_008437139.1|2.4e-20880.58PREDICTED: 7-ethoxycoumarin O-deethylase-like isoform X1 [Cucumis melo][more]
gi|659073584|ref|XP_008437141.1|2.4e-20880.58PREDICTED: 7-ethoxycoumarin O-deethylase-like isoform X2 [Cucumis melo][more]
gi|659073592|ref|XP_008437145.1|4.5e-16766.22PREDICTED: 7-ethoxycoumarin O-deethylase-like [Cucumis melo][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR001128Cyt_P450
IPR002401Cyt_P450_E_grp-I
IPR017972Cyt_P450_CS
Vocabulary: Molecular Function
TermDefinition
GO:0016705oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
GO:0005506iron ion binding
GO:0020037heme binding
Vocabulary: Biological Process
TermDefinition
GO:0055114oxidation-reduction process
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055114 oxidation-reduction process
cellular_component GO:0005575 cellular_component
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsa.302220.1Cucsa.302220.1mRNA


Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001128Cytochrome P450PRINTSPR00385P450coord: 294..305
score: 4.8E-13coord: 375..384
score: 4.8E-13coord: 384..395
score: 4.8E-13coord: 241..258
score: 4.8
IPR001128Cytochrome P450GENE3DG3DSA:1.10.630.10coord: 2..438
score: 1.3E
IPR001128Cytochrome P450PFAMPF00067p450coord: 2..429
score: 5.0
IPR001128Cytochrome P450unknownSSF48264Cytochrome P450coord: 2..438
score: 8.77E
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 250..276
score: 1.9E-42coord: 384..407
score: 1.9E-42coord: 23..44
score: 1.9E-42coord: 230..247
score: 1.9E-42coord: 293..311
score: 1.9E-42coord: 334..358
score: 1.9E-42coord: 374..384
score: 1.9
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 377..386
scor
NoneNo IPR availablePANTHERPTHR24298FAMILY NOT NAMEDcoord: 1..442
score: 1.2E
NoneNo IPR availablePANTHERPTHR24298:SF136SUBFAMILY NOT NAMEDcoord: 1..442
score: 1.2E

The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Cucsa.302220Lsi09G003820Bottle gourd (USVL1VR-Ls)cgylsiB487
Cucsa.302220Carg16694Silver-seed gourdcarcgyB1054
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Cucsa.302220Cucumber (Chinese Long) v3cgycucB465
Cucsa.302220Cucumber (Chinese Long) v3cgycucB466
Cucsa.302220Cucumber (Chinese Long) v3cgycucB470
Cucsa.302220Watermelon (97103) v2cgywmbB520
Cucsa.302220Watermelon (97103) v2cgywmbB521
Cucsa.302220Watermelon (97103) v2cgywmbB522
Cucsa.302220Wax gourdcgywgoB604
Cucsa.302220Wax gourdcgywgoB606
Cucsa.302220Cucumber (Gy14) v1cgycgyB126
Cucsa.302220Cucumber (Gy14) v1cgycgyB141
Cucsa.302220Cucurbita maxima (Rimu)cgycmaB0830
Cucsa.302220Cucurbita maxima (Rimu)cgycmaB0837
Cucsa.302220Cucurbita maxima (Rimu)cgycmaB0839
Cucsa.302220Cucurbita moschata (Rifu)cgycmoB0824
Cucsa.302220Cucurbita moschata (Rifu)cgycmoB0826
Cucsa.302220Cucurbita moschata (Rifu)cgycmoB0832
Cucsa.302220Cucurbita moschata (Rifu)cgycmoB0834
Cucsa.302220Wild cucumber (PI 183967)cgycpiB452
Cucsa.302220Wild cucumber (PI 183967)cgycpiB453
Cucsa.302220Wild cucumber (PI 183967)cgycpiB457
Cucsa.302220Cucumber (Chinese Long) v2cgycuB434
Cucsa.302220Cucumber (Chinese Long) v2cgycuB429
Cucsa.302220Cucumber (Chinese Long) v2cgycuB430
Cucsa.302220Melon (DHL92) v3.5.1cgymeB495
Cucsa.302220Melon (DHL92) v3.5.1cgymeB497
Cucsa.302220Melon (DHL92) v3.5.1cgymeB498
Cucsa.302220Watermelon (Charleston Gray)cgywcgB519
Cucsa.302220Watermelon (Charleston Gray)cgywcgB520
Cucsa.302220Watermelon (Charleston Gray)cgywcgB521
Cucsa.302220Watermelon (97103) v1cgywmB547
Cucsa.302220Watermelon (97103) v1cgywmB549
Cucsa.302220Watermelon (97103) v1cgywmB552
Cucsa.302220Cucurbita pepo (Zucchini)cgycpeB0789
Cucsa.302220Cucurbita pepo (Zucchini)cgycpeB0790
Cucsa.302220Cucurbita pepo (Zucchini)cgycpeB0792
Cucsa.302220Cucurbita pepo (Zucchini)cgycpeB0796
Cucsa.302220Bottle gourd (USVL1VR-Ls)cgylsiB490
Cucsa.302220Bottle gourd (USVL1VR-Ls)cgylsiB491
Cucsa.302220Melon (DHL92) v3.6.1cgymedB494
Cucsa.302220Melon (DHL92) v3.6.1cgymedB496
Cucsa.302220Melon (DHL92) v3.6.1cgymedB497
Cucsa.302220Silver-seed gourdcarcgyB0988
Cucsa.302220Silver-seed gourdcarcgyB1043