Cucsa.244520.1 (mRNA) Cucumber (Gy14) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cucsa.244520.1
Type	mRNA
Organism	Cucumis sativus (Cucumber (Gy14) v1)
Description	Cystathionine beta-synthase (CBS) family protein
Location	scaffold02135 : 378077 .. 380404 (+)
Sequence length	933

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ACGGGGATTTTTCTCTCCATTAAAACGCCTTTTTTtCTCTTGGAAAAGTGTTCTTGTTTCCTATCCTTACACGGATCGGAAGCTAATTACAACCTAATTCTCCGATTCCACCTTCACTCTGAAAATTTCCCGGTTGTATTTCTCTTCCGTTCGCTGGATCAGCCATTGATTCGTTTGAATTTCTCTGTTACTCCTCAATCATCTAGCAAAATGAGCTCCATCTCTCTCTCCAATTCACATCCTCTTGCGCGCCCTTATCCTCATCATCTTCCTCACTCTCACCGTCAACAATGGTGCTCCCGCCCTCTGCTTTCGACCAATTCTCTTTCTAAGCTACACCGGTTTGGGATCTCCGACCGATTCCCCGCTCGACCTCCCTTGCCTTTGGTTCTTGCTTCTTCTGGAGCTGGCGTCGTTGATTCCTTTCCGGTTTGTCATTTCCTTCTCATATTCTTTGTTCTATTCACAACTTAATCACGATTATGTTTTTGTTTTtAGTTTATTTACTGCAAGCCGCACTCTAATTGCTGACCTACCGTTATGTAGTGAATTAGGCGATATCTCTACTCTTCACTAAGATTTGTATGTCTGTGTTCTACCTACGTGAATAAGTATGGACATAACCGCCAAAATATGCTTTCATTTCGGCTGTTAAGTAATATAGAGATTATACTTTTCCTTCACGACGATTCATCCGTGGAAAAGAACCTGTACCGTCTACGATTATGCTGCATAATTTTAGAATGGAGCATAGAACTAAAGCAGACGCTCGAAAGTGCTTATAAACAATTATCGATTTGGTCCTATTGGTGCTACTATGTATAATATAAGCTATATGTTATGTTTATTATATGTTTTGTTTTAGAGAGGGAGAGTTTGGGGAATTGACTTGTTTATGGCTATTTTAGACCTGATGTTTTAAATTTCTGTTTGACGGCTTGGCTGTCGTGTTTTGCTCTTATTTCTATCATGATGATTTTCAGCTCAGAGGAACATATACGGTTGGTGATTTTATGACGAGAAAGGAGAATCTATATGTTGTTAAACCTACAACAACAGTTGATGAAGGTGCGGTTGATTGAGGGACTAAATTTCAATTGTTTTGGTTAGAGTTGCTAATGCTAATTGGTTCTTTCATCGGCTTTTGTGCTTACAGCATTAGAGGTCTTGGTGGAAAAGAGAATAACTGGCTTTCCTGTTGTCGATGATGATTGGAATCTGGTAGAGTCTTTCTTTTCACCTTTTTGTGCTCCTTGCGCGTTTTTTTTtATATTTCCTCTGTCAACTTACTATGGAAGTGAATTACCAAAGTCCGATGATATTCTTGTGGAAGGGACTGGGGAAGGGGTCTTCTCTTTGTCCTCAGTTTCGCTGAGGATTTTGCAAAAATTATCAACATTATTTTTtCTTTCGTTTTTTTGAGGATTTGGGGTGCACTTGTGCATCTATGGTCAAAGCCTTACCCTTAAGTCAGGCCAGGAATTTTGGTCCATTTATATTAAGGGTACGGCTAGCAAAGAATCCAAAGTGCTACTCTTCTTTCTTAATAATTTGGCTTGTGTTTTGCTGTTGCAGGTTGGAGTTGTCTCAGATTATGACTTATTAGCACTTGATTCCATTTCAGGTATTTTCACTTCCCATAGCCCGGGTTTCAACTGAAATCACCATTACACGAGGAATTGTTCTTTGCTTATTTTTCTGTTGAAATTGGATTTCTTTCCTTTTTCATTTGTTAAATTACTAGTCTTTTTTTTtATTCTTCTTATTTTAATTCCTAAATTTGAGATGCTTCTTCAATTTTGCTTTCTTATTTAGTTTCCAATTGGGAAACTTTATTGTAATCACCCATGGGTGCTTGGGGTCTTCTCTTATTTCATTTTATCAATGAAATATTTCTTCTCTAAAAATATTATTTTTtGTTATAATCCATTTAAATCATTGCTTCTCTTAATAATATTTCCCCCCTATTATTGGCAATTAGGTGGTACTCAGAGTGATACCAATCTGTTTCCTGATGTGGATAGTTCGTGGAAAGTATGTAAGCAGTGCCATTTATCTTCTGGTTATTTGCCCTCTTTTTTTTTTTtCACAAACATGAGGATTTTTAACAAAAACACCCTCAGAAAACGTGATCAATCCACTGTTTGGATTGGTTTTCAGCTGTGTGAACCCAAATCAGCAGAAAGTTTTGTTTTGTTTTTTGTTTTAAATAATTTTTTTACTCCAGAATATTGTTCTCAGACCCTTTTGGAAAGTACTCCAAAATATTGTTTGATTGATCTTTGGAGTACTCATTTTCAAAAAaTGAAATATTTATTGATACATTGA

mRNA sequence

ACGGGGATTTTTCTCTCCATTAAAACGCCTTTTTTTCTCTTGGAAAAGTGTTCTTGTTTCCTATCCTTACACGGATCGGAAGCTAATTACAACCTAATTCTCCGATTCCACCTTCACTCTGAAAATTTCCCGGTTGTATTTCTCTTCCGTTCGCTGGATCAGCCATTGATTCGTTTGAATTTCTCTGTTACTCCTCAATCATCTAGCAAAATGAGCTCCATCTCTCTCTCCAATTCACATCCTCTTGCGCGCCCTTATCCTCATCATCTTCCTCACTCTCACCGTCAACAATGGTGCTCCCGCCCTCTGCTTTCGACCAATTCTCTTTCTAAGCTACACCGGTTTGGGATCTCCGACCGATTCCCCGCTCGACCTCCCTTGCCTTTGGTTCTTGCTTCTTCTGGAGCTGGCGTCGTTGATTCCTTTCCGCTCAGAGGAACATATACGGTTGGTGATTTTATGACGAGAAAGGAGAATCTATATGTTGTTAAACCTACAACAACAGTTGATGAAGCATTAGAGGTCTTGGTGGAAAAGAGAATAACTGGCTTTCCTGTTGTCGATGATGATTGGAATCTGGTTGGAGTTGTCTCAGATTATGACTTATTAGCACTTGATTCCATTTCAGGTGGTACTCAGAGTGATACCAATCTGTTTCCTGATGTGGATAGTTCGTGGAAAGTATGTAAGCAGTGCCATTTATCTTCTGGTTATTTGCCCTCTTTTTTTTTTTTCACAAACATGAGGATTTTTAACAAAAACACCCTCAGAAAACGTGATCAATCCACTGTTTGGATTGGTTTTCAGCTGTGTGAACCCAAATCAGCAGAAAAATATTGTTCTCAGACCCTTTTGGAAAGTACTCCAAAATATTGTTTGATTGATCTTTGGAGTACTCATTTTCAAAAAATGAAATATTTATTGATACATTGA

Coding sequence (CDS)

ACGGGGATTTTTCTCTCCATTAAAACGCCTTTTTTtCTCTTGGAAAAGTGTTCTTGTTTCCTATCCTTACACGGATCGGAAGCTAATTACAACCTAATTCTCCGATTCCACCTTCACTCTGAAAATTTCCCGGTTGTATTTCTCTTCCGTTCGCTGGATCAGCCATTGATTCGTTTGAATTTCTCTGTTACTCCTCAATCATCTAGCAAAATGAGCTCCATCTCTCTCTCCAATTCACATCCTCTTGCGCGCCCTTATCCTCATCATCTTCCTCACTCTCACCGTCAACAATGGTGCTCCCGCCCTCTGCTTTCGACCAATTCTCTTTCTAAGCTACACCGGTTTGGGATCTCCGACCGATTCCCCGCTCGACCTCCCTTGCCTTTGGTTCTTGCTTCTTCTGGAGCTGGCGTCGTTGATTCCTTTCCGCTCAGAGGAACATATACGGTTGGTGATTTTATGACGAGAAAGGAGAATCTATATGTTGTTAAACCTACAACAACAGTTGATGAAGCATTAGAGGTCTTGGTGGAAAAGAGAATAACTGGCTTTCCTGTTGTCGATGATGATTGGAATCTGGTTGGAGTTGTCTCAGATTATGACTTATTAGCACTTGATTCCATTTCAGGTGGTACTCAGAGTGATACCAATCTGTTTCCTGATGTGGATAGTTCGTGGAAAGTATGTAAGCAGTGCCATTTATCTTCTGGTTATTTGCCCTCTTTTTTTTTTTtCACAAACATGAGGATTTTTAACAAAAACACCCTCAGAAAACGTGATCAATCCACTGTTTGGATTGGTTTTCAGCTGTGTGAACCCAAATCAGCAGAAAAATATTGTTCTCAGACCCTTTTGGAAAGTACTCCAAAATATTGTTTGATTGATCTTTGGAGTACTCATTTTCAAAAAaTGAAATATTTATTGATACATTGA

Protein sequence

TGIFLSIKTPFFLLEKCSCFLSLHGSEANYNLILRFHLHSENFPVVFLFRSLDQPLIRLNFSVTPQSSSKMSSISLSNSHPLARPYPHHLPHSHRQQWCSRPLLSTNSLSKLHRFGISDRFPARPPLPLVLASSGAGVVDSFPLRGTYTVGDFMTRKENLYVVKPTTTVDEALEVLVEKRITGFPVVDDDWNLVGVVSDYDLLALDSISGGTQSDTNLFPDVDSSWKVCKQCHLSSGYLPSFFFFTNMRIFNKNTLRKRDQSTVWIGFQLCEPKSAEKYCSQTLLESTPKYCLIDLWSTHFQKMKYLLIH*

Homology

[Close]

BLAST of Cucsa.244520.1 vs. Swiss-Prot
Match: CBSX1_ARATH (CBS domain-containing protein CBSX1, chloroplastic OS=Arabidopsis thaliana GN=CBSX1 PE=1 SV=2)

HSP 1 Score: 150.6 bits (379), Expect = 2.8e-35
Identity = 71/122 (58.20%), Postives = 97/122 (79.51%), Query Frame = 1


Query: 108 SLSKLHRFGISDRFPARPPLPLVLASSGAGVV--DSFPLRGTYTVGDFMTRKENLYVVKP 167
           S+   H+F  S  FP++  +P   +++G+ ++   S P  G YTVG+FMT+KE+L+VVKP
Sbjct: 33  SVQPCHKFTFSRSFPSKSRIPSASSAAGSTLMTNSSSPRSGVYTVGEFMTKKEDLHVVKP 92

Query: 168 TTTVDEALEVLVEKRITGFPVVDDDWNLVGVVSDYDLLALDSISGGTQSDTNLFPDVDSS 227
           TTTVDEALE+LVE RITGFPV+D+DW LVG+VSDYDLLALDSISG  +++ ++FP+VDS+
Sbjct: 93  TTTVDEALELLVENRITGFPVIDEDWKLVGLVSDYDLLALDSISGSGRTENSMFPEVDST 152

HSP 2 Score: 37.4 bits (85), Expect = 3.5e-01
Identity = 19/49 (38.78%), Postives = 30/49 (61.22%), Query Frame = 1


Query: 150 VGDFMTRKENLYVVKPTTTVDEALEVLVEKRITGFPVVDDDWNLVGVVS 199
           VGD MT      VV+  T +++A ++L+E +    PVVD D  LVG+++
Sbjct: 171 VGDLMTPAP--LVVEEKTNLEDAAKILLETKYRRLPVVDSDGKLVGIIT 217

[Close]

BLAST of Cucsa.244520.1 vs. Swiss-Prot
Match: CBSX2_ARATH (CBS domain-containing protein CBSX2, chloroplastic OS=Arabidopsis thaliana GN=CBSX2 PE=1 SV=1)

HSP 1 Score: 145.6 bits (366), Expect = 9.1e-34
Identity = 87/162 (53.70%), Postives = 110/162 (67.90%), Query Frame = 1


Query: 71  MSSISLSNSHPLAR-PYPHHLPHSHRQQWCSRPLLSTNSLSKL---HRFGISDRFPARPP 130
           M SISLSNS P+ R P    L H       S   +S++S S L   +R   S   P+   
Sbjct: 1   MGSISLSNSMPITRLPLLTSLYHQ------SFLPISSSSFSLLPLSNRRRSSTFSPSITV 60

Query: 131 LPLVLASSGAGVVDSFPLR-GTYTVGDFMTRKENLYVVKPTTTVDEALEVLVEKRITGFP 190
                A +     +S P + G YTVGDFMT ++NL+VVKP+T+VD+ALE+LVEK++TG P
Sbjct: 61  SAFFAAPASVNNNNSVPAKNGGYTVGDFMTPRQNLHVVKPSTSVDDALELLVEKKVTGLP 120

Query: 191 VVDDDWNLVGVVSDYDLLALDSISGGTQSDTNLFPDVDSSWK 228
           V+DD+W LVGVVSDYDLLALDSISG +Q+DTNLFPDVDS+WK
Sbjct: 121 VIDDNWTLVGVVSDYDLLALDSISGRSQNDTNLFPDVDSTWK 156

HSP 2 Score: 36.2 bits (82), Expect = 7.8e-01
Identity = 18/49 (36.73%), Postives = 30/49 (61.22%), Query Frame = 1


Query: 150 VGDFMTRKENLYVVKPTTTVDEALEVLVEKRITGFPVVDDDWNLVGVVS 199
           VGD MT      VV+ +T +++A  +L+E +    PVVD D  L+G+++
Sbjct: 173 VGDLMTPSP--LVVRDSTNLEDAARLLLETKFRRLPVVDADGKLIGILT 219

[Close]

BLAST of Cucsa.244520.1 vs. TrEMBL
Match: A0A0A0L716_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G202220 PE=4 SV=1)

HSP 1 Score: 323.6 bits (828), Expect = 2.7e-85
Identity = 157/157 (100.00%), Postives = 157/157 (100.00%), Query Frame = 1


Query: 71  MSSISLSNSHPLARPYPHHLPHSHRQQWCSRPLLSTNSLSKLHRFGISDRFPARPPLPLV 130
           MSSISLSNSHPLARPYPHHLPHSHRQQWCSRPLLSTNSLSKLHRFGISDRFPARPPLPLV
Sbjct: 1   MSSISLSNSHPLARPYPHHLPHSHRQQWCSRPLLSTNSLSKLHRFGISDRFPARPPLPLV 60

Query: 131 LASSGAGVVDSFPLRGTYTVGDFMTRKENLYVVKPTTTVDEALEVLVEKRITGFPVVDDD 190
           LASSGAGVVDSFPLRGTYTVGDFMTRKENLYVVKPTTTVDEALEVLVEKRITGFPVVDDD
Sbjct: 61  LASSGAGVVDSFPLRGTYTVGDFMTRKENLYVVKPTTTVDEALEVLVEKRITGFPVVDDD 120

Query: 191 WNLVGVVSDYDLLALDSISGGTQSDTNLFPDVDSSWK 228
           WNLVGVVSDYDLLALDSISGGTQSDTNLFPDVDSSWK
Sbjct: 121 WNLVGVVSDYDLLALDSISGGTQSDTNLFPDVDSSWK 157

[Close]

BLAST of Cucsa.244520.1 vs. TrEMBL
Match: A0A0A0L716_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G202220 PE=4 SV=1)

HSP 1 Score: 35.8 bits (81), Expect = 1.1e+02
Identity = 18/49 (36.73%), Postives = 29/49 (59.18%), Query Frame = 1


Query: 150 VGDFMTRKENLYVVKPTTTVDEALEVLVEKRITGFPVVDDDWNLVGVVS 199
           VGD MT       V+ T+ +++A  +L+E +    PVVD D  LVG+++
Sbjct: 174 VGDLMTSSP--LAVRETSNLEDAARLLLETKYRRLPVVDADGKLVGIIT 220

HSP 2 Score: 172.2 bits (435), Expect = 1.0e-39
Identity = 92/127 (72.44%), Postives = 100/127 (78.74%), Query Frame = 1


Query: 102 PLLSTNSLSKLHRFGISDRFPARPPLPLVLASSGAGVVDSFPLRG-TYTVGDFMTRKENL 161
           P   +  L +  RF  SDRF +R   P + ASSGA V +S P R  TYTVGDFMTRKENL
Sbjct: 29  PAHQSRKLLQRRRFDASDRF-SRSLRPPIAASSGAHVANSVPSRNKTYTVGDFMTRKENL 88

Query: 162 YVVKPTTTVDEALEVLVEKRITGFPVVDDDWNLVGVVSDYDLLALDSISGGTQSDTNLFP 221
            VVKPTTTVDEALE LVEKRITGFPV+DDDW LVGVVSDYDLLALDSISGG Q DT+LFP
Sbjct: 89  LVVKPTTTVDEALETLVEKRITGFPVIDDDWKLVGVVSDYDLLALDSISGGGQCDTSLFP 148

Query: 222 DVDSSWK 228
           +VDSSWK
Sbjct: 149 NVDSSWK 154

[Close]

BLAST of Cucsa.244520.1 vs. TrEMBL
Match: W9S667_9ROSA (CBS domain-containing protein CBSX1 OS=Morus notabilis GN=L484_027493 PE=4 SV=1)

HSP 1 Score: 36.2 bits (82), Expect = 8.7e+01
Identity = 19/49 (38.78%), Postives = 29/49 (59.18%), Query Frame = 1


Query: 150 VGDFMTRKENLYVVKPTTTVDEALEVLVEKRITGFPVVDDDWNLVGVVS 199
           VGD MT      VV  TT +++A  +L+E +    PVVD +  LVG+++
Sbjct: 171 VGDLMTSAP--LVVHETTNLEDAARLLLETKYRRLPVVDGEGKLVGIIT 217

HSP 2 Score: 166.4 bits (420), Expect = 5.6e-38
Identity = 80/104 (76.92%), Postives = 94/104 (90.38%), Query Frame = 1


Query: 129 LVLASSGAGVVDSFPLR-GTYTVGDFMTRKENLYVVKPTTTVDEALEVLVEKRITGFPVV 188
           + +A++GA V +S P R G+YTVGDFMTRKE+L+VVK TTTVDEALE+LVEKRITGFPV+
Sbjct: 59  VTVAAAGASVTNSVPPRNGSYTVGDFMTRKEDLHVVKATTTVDEALEMLVEKRITGFPVI 118

Query: 189 DDDWNLVGVVSDYDLLALDSISGGTQSDTNLFPDVDSSWKVCKQ 232
           DD+WNLVGVVSDYDLLALDSISGG Q D N+FPDVDSSWKVC++
Sbjct: 119 DDEWNLVGVVSDYDLLALDSISGGAQGDANIFPDVDSSWKVCRR 162

[Close]

BLAST of Cucsa.244520.1 vs. TrEMBL
Match: A0A059AMD8_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_I01128 PE=4 SV=1)

HSP 1 Score: 166.0 bits (419), Expect = 7.3e-38
Identity = 81/104 (77.88%), Postives = 93/104 (89.42%), Query Frame = 1


Query: 129 LVLASSGAGVVDSFPLR-GTYTVGDFMTRKENLYVVKPTTTVDEALEVLVEKRITGFPVV 188
           + +A++GA V +S P R G+YTVGDFMTRKE+L+VVK TTTVDEALE+LVEKRITGFPV+
Sbjct: 59  VTVAAAGASVTNSVPPRNGSYTVGDFMTRKEDLHVVKATTTVDEALEMLVEKRITGFPVI 118

Query: 189 DDDWNLVGVVSDYDLLALDSISGGTQSDTNLFPDVDSSWKVCKQ 232
           DD+WNLVGVVSDYDLLALDSISGG Q D N+FPDVDSSWKVC Q
Sbjct: 119 DDEWNLVGVVSDYDLLALDSISGGAQGDANIFPDVDSSWKVCFQ 162

[Close]

BLAST of Cucsa.244520.1 vs. TrEMBL
Match: M5VMP1_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa010834mg PE=4 SV=1)

HSP 1 Score: 162.5 bits (410), Expect = 8.0e-37
Identity = 90/129 (69.77%), Postives = 101/129 (78.29%), Query Frame = 1


Query: 106 TNSLSKLHRF---GISDRFPARPPLPLVLASSGAGVV-DSFPLRGTYTVGDFMTRKENLY 165
           ++SL  L RF    +S R  +    PLVLASS AGV  +S P  GT+TVGDFMT KE L+
Sbjct: 26  SSSLLPLRRFPLLALSKRRRSLQFRPLVLASSAAGVASNSLPRNGTFTVGDFMTTKEYLH 85

Query: 166 VVKPTTTVDEALEVLVEKRITGFPVVDDDWNLVGVVSDYDLLALDSISG---GTQSDTNL 225
           VVKP+TTVD+AL+ LVEKRITGFPV+DDDW LVGVVSDYDLLALDSISG   G QSDTNL
Sbjct: 86  VVKPSTTVDQALDALVEKRITGFPVIDDDWKLVGVVSDYDLLALDSISGNIKGGQSDTNL 145

Query: 226 FPDVDSSWK 228
           FPDVDSSWK
Sbjct: 146 FPDVDSSWK 154

[Close]

BLAST of Cucsa.244520.1 vs. TAIR10
Match: AT4G36910.1 (AT4G36910.1 Cystathionine beta-synthase (CBS) family protein)

HSP 1 Score: 150.6 bits (379), Expect = 1.6e-36
Identity = 71/122 (58.20%), Postives = 97/122 (79.51%), Query Frame = 1


Query: 108 SLSKLHRFGISDRFPARPPLPLVLASSGAGVV--DSFPLRGTYTVGDFMTRKENLYVVKP 167
           S+   H+F  S  FP++  +P   +++G+ ++   S P  G YTVG+FMT+KE+L+VVKP
Sbjct: 33  SVQPCHKFTFSRSFPSKSRIPSASSAAGSTLMTNSSSPRSGVYTVGEFMTKKEDLHVVKP 92

Query: 168 TTTVDEALEVLVEKRITGFPVVDDDWNLVGVVSDYDLLALDSISGGTQSDTNLFPDVDSS 227
           TTTVDEALE+LVE RITGFPV+D+DW LVG+VSDYDLLALDSISG  +++ ++FP+VDS+
Sbjct: 93  TTTVDEALELLVENRITGFPVIDEDWKLVGLVSDYDLLALDSISGSGRTENSMFPEVDST 152

HSP 2 Score: 37.4 bits (85), Expect = 2.0e-02
Identity = 19/49 (38.78%), Postives = 30/49 (61.22%), Query Frame = 1


Query: 150 VGDFMTRKENLYVVKPTTTVDEALEVLVEKRITGFPVVDDDWNLVGVVS 199
           VGD MT      VV+  T +++A ++L+E +    PVVD D  LVG+++
Sbjct: 171 VGDLMTPAP--LVVEEKTNLEDAAKILLETKYRRLPVVDSDGKLVGIIT 217

[Close]

BLAST of Cucsa.244520.1 vs. TAIR10
Match: AT4G34120.1 (AT4G34120.1 Cystathionine beta-synthase (CBS) family protein)

HSP 1 Score: 145.6 bits (366), Expect = 5.1e-35
Identity = 87/162 (53.70%), Postives = 110/162 (67.90%), Query Frame = 1


Query: 71  MSSISLSNSHPLAR-PYPHHLPHSHRQQWCSRPLLSTNSLSKL---HRFGISDRFPARPP 130
           M SISLSNS P+ R P    L H       S   +S++S S L   +R   S   P+   
Sbjct: 1   MGSISLSNSMPITRLPLLTSLYHQ------SFLPISSSSFSLLPLSNRRRSSTFSPSITV 60

Query: 131 LPLVLASSGAGVVDSFPLR-GTYTVGDFMTRKENLYVVKPTTTVDEALEVLVEKRITGFP 190
                A +     +S P + G YTVGDFMT ++NL+VVKP+T+VD+ALE+LVEK++TG P
Sbjct: 61  SAFFAAPASVNNNNSVPAKNGGYTVGDFMTPRQNLHVVKPSTSVDDALELLVEKKVTGLP 120

Query: 191 VVDDDWNLVGVVSDYDLLALDSISGGTQSDTNLFPDVDSSWK 228
           V+DD+W LVGVVSDYDLLALDSISG +Q+DTNLFPDVDS+WK
Sbjct: 121 VIDDNWTLVGVVSDYDLLALDSISGRSQNDTNLFPDVDSTWK 156

HSP 2 Score: 36.2 bits (82), Expect = 4.4e-02
Identity = 18/49 (36.73%), Postives = 30/49 (61.22%), Query Frame = 1


Query: 150 VGDFMTRKENLYVVKPTTTVDEALEVLVEKRITGFPVVDDDWNLVGVVS 199
           VGD MT      VV+ +T +++A  +L+E +    PVVD D  L+G+++
Sbjct: 173 VGDLMTPSP--LVVRDSTNLEDAARLLLETKFRRLPVVDADGKLIGILT 219

[Close]

BLAST of Cucsa.244520.1 vs. NCBI nr
Match: gi|449445894|ref|XP_004140707.1| (PREDICTED: CBS domain-containing protein CBSX1, chloroplastic-like [Cucumis sativus])

HSP 1 Score: 323.6 bits (828), Expect = 3.9e-85
Identity = 157/157 (100.00%), Postives = 157/157 (100.00%), Query Frame = 1


Query: 71  MSSISLSNSHPLARPYPHHLPHSHRQQWCSRPLLSTNSLSKLHRFGISDRFPARPPLPLV 130
           MSSISLSNSHPLARPYPHHLPHSHRQQWCSRPLLSTNSLSKLHRFGISDRFPARPPLPLV
Sbjct: 1   MSSISLSNSHPLARPYPHHLPHSHRQQWCSRPLLSTNSLSKLHRFGISDRFPARPPLPLV 60

Query: 131 LASSGAGVVDSFPLRGTYTVGDFMTRKENLYVVKPTTTVDEALEVLVEKRITGFPVVDDD 190
           LASSGAGVVDSFPLRGTYTVGDFMTRKENLYVVKPTTTVDEALEVLVEKRITGFPVVDDD
Sbjct: 61  LASSGAGVVDSFPLRGTYTVGDFMTRKENLYVVKPTTTVDEALEVLVEKRITGFPVVDDD 120

Query: 191 WNLVGVVSDYDLLALDSISGGTQSDTNLFPDVDSSWK 228
           WNLVGVVSDYDLLALDSISGGTQSDTNLFPDVDSSWK
Sbjct: 121 WNLVGVVSDYDLLALDSISGGTQSDTNLFPDVDSSWK 157

[Close]

BLAST of Cucsa.244520.1 vs. NCBI nr
Match: gi|449445894|ref|XP_004140707.1| (PREDICTED: CBS domain-containing protein CBSX1, chloroplastic-like [Cucumis sativus])

HSP 1 Score: 35.8 bits (81), Expect = 1.6e+02
Identity = 18/49 (36.73%), Postives = 29/49 (59.18%), Query Frame = 1


Query: 150 VGDFMTRKENLYVVKPTTTVDEALEVLVEKRITGFPVVDDDWNLVGVVS 199
           VGD MT       V+ T+ +++A  +L+E +    PVVD D  LVG+++
Sbjct: 174 VGDLMTSSP--LAVRETSNLEDAARLLLETKYRRLPVVDADGKLVGIIT 220

HSP 2 Score: 317.8 bits (813), Expect = 2.1e-83
Identity = 154/157 (98.09%), Postives = 156/157 (99.36%), Query Frame = 1


Query: 71  MSSISLSNSHPLARPYPHHLPHSHRQQWCSRPLLSTNSLSKLHRFGISDRFPARPPLPLV 130
           M+SISLSNSH LARPYPHHLPHSHRQQWCSRPLLS+NSLSKLHRFGISDRFPARPPLPLV
Sbjct: 1   MTSISLSNSHALARPYPHHLPHSHRQQWCSRPLLSSNSLSKLHRFGISDRFPARPPLPLV 60

Query: 131 LASSGAGVVDSFPLRGTYTVGDFMTRKENLYVVKPTTTVDEALEVLVEKRITGFPVVDDD 190
           LASSGAGVVDSFPLRGTYTVGDFMTRKENLYVVKPTTTVDEALEVLVEKRITGFPVVDDD
Sbjct: 61  LASSGAGVVDSFPLRGTYTVGDFMTRKENLYVVKPTTTVDEALEVLVEKRITGFPVVDDD 120

Query: 191 WNLVGVVSDYDLLALDSISGGTQSDTNLFPDVDSSWK 228
           WNLVGVVSDYDLLALDSISGGTQSDTNLFPDVDSSWK
Sbjct: 121 WNLVGVVSDYDLLALDSISGGTQSDTNLFPDVDSSWK 157

[Close]

BLAST of Cucsa.244520.1 vs. NCBI nr
Match: gi|659112269|ref|XP_008456144.1| (PREDICTED: CBS domain-containing protein CBSX1, chloroplastic-like [Cucumis melo])

HSP 1 Score: 35.8 bits (81), Expect = 1.6e+02
Identity = 18/49 (36.73%), Postives = 29/49 (59.18%), Query Frame = 1


Query: 150 VGDFMTRKENLYVVKPTTTVDEALEVLVEKRITGFPVVDDDWNLVGVVS 199
           VGD MT       V+ T+ +++A  +L+E +    PVVD D  LVG+++
Sbjct: 174 VGDLMTSSP--LAVRETSNLEDAARLLLETKYRRLPVVDADGKLVGIIT 220

HSP 2 Score: 179.1 bits (453), Expect = 1.2e-41
Identity = 103/164 (62.80%), Postives = 117/164 (71.34%), Query Frame = 1


Query: 67  SSSKMSSISLSNSHPLARPYPHHLP--HSHRQQWCSRPLLSTNSLSKLHRFGISDRFPAR 126
           S S  S I L  +H  + P     P  HSH      RPL    SLS  HR   S  F   
Sbjct: 18  SGSANSPILLLRNHHRSLPSSFLFPCLHSH-----PRPLPIPPSLSTRHRLTSSRGFSGN 77

Query: 127 PPLPLVLASSGAGVVDSFPLR-GTYTVGDFMTRKENLYVVKPTTTVDEALEVLVEKRITG 186
            P   V+ASSG+ VV+S P R GTYTVGDFMT+K+NL+VVKP+TTVDEAL+ LVEKRITG
Sbjct: 78  RP-SAVVASSGSHVVNSLPPRNGTYTVGDFMTKKDNLHVVKPSTTVDEALQALVEKRITG 137

Query: 187 FPVVDDDWNLVGVVSDYDLLALDSISGGTQSDTNLFPDVDSSWK 228
           FPV+DDDW LVGVVSDYDLLALDSISGG+Q+DTNLFP+VDSSWK
Sbjct: 138 FPVIDDDWKLVGVVSDYDLLALDSISGGSQNDTNLFPNVDSSWK 175

[Close]

BLAST of Cucsa.244520.1 vs. NCBI nr
Match: gi|1009150146|ref|XP_015892863.1| (PREDICTED: CBS domain-containing protein CBSX1, chloroplastic-like isoform X1 [Ziziphus jujuba])

HSP 1 Score: 38.9 bits (89), Expect = 1.9e+01
Identity = 20/49 (40.82%), Postives = 30/49 (61.22%), Query Frame = 1


Query: 150 VGDFMTRKENLYVVKPTTTVDEALEVLVEKRITGFPVVDDDWNLVGVVS 199
           VGD MT      VV+ TT +++A  +L+E +    PVVD D  LVG+++
Sbjct: 192 VGDLMTTAP--LVVRETTNLEDAARLLLETKYRRLPVVDGDGKLVGIIT 238

HSP 2 Score: 172.2 bits (435), Expect = 1.5e-39
Identity = 92/127 (72.44%), Postives = 100/127 (78.74%), Query Frame = 1


Query: 102 PLLSTNSLSKLHRFGISDRFPARPPLPLVLASSGAGVVDSFPLRG-TYTVGDFMTRKENL 161
           P   +  L +  RF  SDRF +R   P + ASSGA V +S P R  TYTVGDFMTRKENL
Sbjct: 29  PAHQSRKLLQRRRFDASDRF-SRSLRPPIAASSGAHVANSVPSRNKTYTVGDFMTRKENL 88

Query: 162 YVVKPTTTVDEALEVLVEKRITGFPVVDDDWNLVGVVSDYDLLALDSISGGTQSDTNLFP 221
            VVKPTTTVDEALE LVEKRITGFPV+DDDW LVGVVSDYDLLALDSISGG Q DT+LFP
Sbjct: 89  LVVKPTTTVDEALETLVEKRITGFPVIDDDWKLVGVVSDYDLLALDSISGGGQCDTSLFP 148

Query: 222 DVDSSWK 228
           +VDSSWK
Sbjct: 149 NVDSSWK 154

[Close]

BLAST of Cucsa.244520.1 vs. NCBI nr
Match: gi|703143017|ref|XP_010107905.1| (CBS domain-containing protein CBSX1 [Morus notabilis])

HSP 1 Score: 36.2 bits (82), Expect = 1.2e+02
Identity = 19/49 (38.78%), Postives = 29/49 (59.18%), Query Frame = 1


Query: 150 VGDFMTRKENLYVVKPTTTVDEALEVLVEKRITGFPVVDDDWNLVGVVS 199
           VGD MT      VV  TT +++A  +L+E +    PVVD +  LVG+++
Sbjct: 171 VGDLMTSAP--LVVHETTNLEDAARLLLETKYRRLPVVDGEGKLVGIIT 217

HSP 2 Score: 166.4 bits (420), Expect = 8.0e-38
Identity = 80/104 (76.92%), Postives = 94/104 (90.38%), Query Frame = 1


Query: 129 LVLASSGAGVVDSFPLR-GTYTVGDFMTRKENLYVVKPTTTVDEALEVLVEKRITGFPVV 188
           + +A++GA V +S P R G+YTVGDFMTRKE+L+VVK TTTVDEALE+LVEKRITGFPV+
Sbjct: 59  VTVAAAGASVTNSVPPRNGSYTVGDFMTRKEDLHVVKATTTVDEALEMLVEKRITGFPVI 118

Query: 189 DDDWNLVGVVSDYDLLALDSISGGTQSDTNLFPDVDSSWKVCKQ 232
           DD+WNLVGVVSDYDLLALDSISGG Q D N+FPDVDSSWKVC++
Sbjct: 119 DDEWNLVGVVSDYDLLALDSISGGAQGDANIFPDVDSSWKVCRR 162

The following BLAST results are available for this feature:

BLAST of Cucsa.244520.1 vs. Swiss-Prot


Match Name	E-value	Identity	Description
CBSX1_ARATH	2.8e-35	58.20	CBS domain-containing protein CBSX1, chloroplastic OS=Arabidopsis thaliana GN=CB...	[more]
CBSX2_ARATH	9.1e-34	53.70	CBS domain-containing protein CBSX2, chloroplastic OS=Arabidopsis thaliana GN=CB...	[more]

BLAST of Cucsa.244520.1 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L716_CUCSA	2.7e-85	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G202220 PE=4 SV=1	[more]
A0A0A0L716_CUCSA	1.1e+02	36.73	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G202220 PE=4 SV=1	[more]
W9S667_9ROSA	8.7e+01	38.78	CBS domain-containing protein CBSX1 OS=Morus notabilis GN=L484_027493 PE=4 SV=1	[more]
A0A059AMD8_EUCGR	7.3e-38	77.88	Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_I01128 PE=4 SV=1	[more]
M5VMP1_PRUPE	8.0e-37	69.77	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa010834mg PE=4 SV=1	[more]

BLAST of Cucsa.244520.1 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G36910.1	1.6e-36	58.20	Cystathionine beta-synthase (CBS) family protein	[more]
AT4G34120.1	5.1e-35	53.70	Cystathionine beta-synthase (CBS) family protein	[more]

BLAST of Cucsa.244520.1 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449445894\|ref\|XP_004140707.1\|	3.9e-85	100.00	PREDICTED: CBS domain-containing protein CBSX1, chloroplastic-like [Cucumis sati...	[more]
gi\|449445894\|ref\|XP_004140707.1\|	1.6e+02	36.73	PREDICTED: CBS domain-containing protein CBSX1, chloroplastic-like [Cucumis sati...	[more]
gi\|659112269\|ref\|XP_008456144.1\|	1.6e+02	36.73	PREDICTED: CBS domain-containing protein CBSX1, chloroplastic-like [Cucumis melo...	[more]
gi\|1009150146\|ref\|XP_015892863.1\|	1.9e+01	40.82	PREDICTED: CBS domain-containing protein CBSX1, chloroplastic-like isoform X1 [Z...	[more]
gi\|703143017\|ref\|XP_010107905.1\|	1.2e+02	38.78	CBS domain-containing protein CBSX1 [Morus notabilis]	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **INTERPRO**
Term	Definition
IPR000644	CBS_dom

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0045454	cell redox homeostasis
cellular_component	GO:0009570	chloroplast stroma
molecular_function	GO:0003674	molecular_function

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsa.244520	Cucsa.244520	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsa.244520.1	Cucsa.244520.1-protein	polypeptide

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsa.244520.1.CDS.1	Cucsa.244520.1.CDS.1	CDS
Cucsa.244520.1.CDS.2	Cucsa.244520.1.CDS.2	CDS
Cucsa.244520.1.CDS.3	Cucsa.244520.1.CDS.3	CDS
Cucsa.244520.1.CDS.4	Cucsa.244520.1.CDS.4	CDS
Cucsa.244520.1.CDS.5	Cucsa.244520.1.CDS.5	CDS
Cucsa.244520.1.CDS.6	Cucsa.244520.1.CDS.6	CDS

InterPro

Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000644	CBS domain	PFAM	PF00571	CBS	coord: 150..204 score: 1.8
IPR000644	CBS domain	SMART	SM00116	cbs_1	coord: 159..207 score: 2.
IPR000644	CBS domain	PROFILE	PS51371	CBS	coord: 154..214 score: 13
None	No IPR available	GENE3D	G3DSA:3.10.580.10		coord: 148..204 score: 1.4
None	No IPR available	PANTHER	PTHR11911	INOSINE-5-MONOPHOSPHATE DEHYDROGENASE RELATED	coord: 143..227 score: 2.3
None	No IPR available	PANTHER	PTHR11911:SF109	SUBFAMILY NOT NAMED	coord: 143..227 score: 2.3
None	No IPR available	unknown	SSF54631	CBS-domain pair	coord: 146..211 score: 4.59