Cucsa.194310.1 (mRNA) Cucumber (Gy14) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cucsa.194310.1
Type	mRNA
Organism	Cucumis sativus (Cucumber (Gy14) v1)
Description	Pectate lyase
Location	scaffold01337 : 694824 .. 698779 (-)
Sequence length	2162

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CTTTCACTGACATCACCACTTCTCGTTTTCCTCTCTTCTTCTTCTTCTTCTTCCCTTTCCTTTTTTCTCTCTATATTTCTTCTGCTTCCTATAATCTCTCTCTCTCTCTACATTTCGTGCTGTTTTTTCTTTTGACGAGAGAGAGAGAGATAGCAGTTATGGCGGCACTTACTAAATGGTCTTCTCTGTCTCTCTTTGCCCTTGCATTGCTGTTTTTCCTAACAATCGCTCTCGTTCGCAAAGAAGAGACCTCCAAAATTAGGTTCGTTTTCTTTTTCTCTCTTAGAAATCAATTAGATGTGTTTTATATGGTAATTAATTCTAGCCACACTATGTTAATTTCTGCTTACGAGTAGTAAATGCAAGCTTCGCATAGTACCGAACTTCTTTTCGATTGGGAGAATCTTGAATGTTCAATGTGGAATTGTGAGTGTATGATTTACTGTTTTTTCATATGATTTTGGATCAATGTAGCAATATAGAAACGTTATAGAGAAGAAGTAAAGCCTTTAATGGAGTCCTTAAGTTCAACAATTTTGGAAGAAAAGAAACGAGGAATAACGCGAGAATCTCTAAATTCGAAACGCGAACTGCGGTGATTATGTTTTTTATTCCTCTCTTATTATTTTTTAAAAATCAGGACTGTGTCAGCGGCGGAGTTTCAGAGCTCCAGCAACTCATCAATGGCGACTAGGTCAGTGTTCGTTTCACTAAACTCAACTAAATAAAAAGAGGTGAAATTCTGTACCGAACAAAACGATCTCGGCCGTGCGTTTTACCCATCATGCTAATCAGAACCGTTGGATTCTCCTCGGATCCGTGTGGTGTGATGATCCAACTGTAGGTAACGATCTCAACCGTTGAGATCGGGATCCTTGATCTGGACCGTTTGTTCCGTTAACAGTTACTCCCTCTGCTCCAGTGAAATAAATACCACAGTTTATTTCCGTAATTACCCCAAGAGGGTCTTAATTTCCCCAGATTGTTCGATAAAATTCCGGGGTATTTCAGTCATCTTGCCCCTAGGAATAAAAGGACAATTTTCTTTATCTTTTTTCTTTTTAATTCTTTAGGCGGTCGTTTCTCATTTTCCCCATCTCGTGCCGACCAATCGTTCCGCCTCTAATTTCTCTCTCTAAACAAAAGCACTTTTTCAAACGGTTTTCTTCTTCCTGACAGAACGACAGTACGGTAAGGGGCGTAAGAGATCTCTTCCGCACCCACCTTGAGCGTCGAGCCATTAACTTCTTCATTTCATTTTCATTCCTCACGCGCCCACTGATATTCACGCGCTTCTACACTCACATATCTGTTCAGTGTGTTTTAACAGATGGCCTTTGTCACTTTATTGGGTGTTTCTGTTTTCAGGGTGGAGAATTATGATGTGGAGCAAGAACTCAACAATGAACATGCTGTGGATAATCCTGATGAGATTGCTGCTTCTGTTGAGATGTGGGTCTTTTTCTTTTATTGCTTCCATACTCGAATTTATGATTTTTTTTACAAATCTGGGTTATCATTACATGCTCAAATCTTGTTTAAGGAAGTCATCATTAGACTATAGAGAAATTACTTTTATTGAAATTTGGGTCGAATTGGATTTTTTGCTCGTTGTAATGAAATAGAACACATTGCGGATGGTGGGCTTTGTTTTTTGTCTGTATAACAAATTCATAGGATGGGCATTTCTAGACGTTATAATGAAAGAGACCTCATGGTCAAAATGCCAGCATGGTTTTGTCTTGTTCTGTTCGTGTAGTATGGCATTTTCTTGTAGCCGTTGGGATGAAAAATCAGCCCTACCGGTCTCCCCTGTCATTAGGTTCCCGACAAATCCTGTTTTTTCTTTCTATTTTTTCCTCGAGATTTGTACTTGTATCAAGTTTCACTACAATTTCAATGAGAGTTCATATTAGGATCCCTTTTTTATATGGCAGGAGCATCCGTAATAGCACTGAAAGGAGGAAGTTGGGATTCTTCTCCTGTGGAACAGGGAATCCCATTGACGACTGCTGGCGTTGTGACTCCAATTGGCACCGTAACCGTAAACGCCTTGCGGAATGTGGCATTGGATTTGGGCGGAATGCTATTGGTGGACGTGATGGCCGTTTCTATGTTGTCACTGATTCGAGTGACAACGATCCGGTGAACCCGAAGCCAGGGACTCTTCGTCATGCTGTTATTCAAGAGAAACCTCTTTGGATCGTGTTCAAAAGGGATATGGTGATTCGATTGAAACAGGAACTCATCATGAACAGTTTTAAGACAATTGATGCTCGAGGGGTAAATGTACACATTGCTAATGGCGCTTGCATCACAATCCAGTTTGTGACTAACATCATTATCCATGGTCTACACATCCATGATTGCAAACCCACAGGGAATGCCATGGTGAGAAGCTCTCCATCTCATTTTGGGTGGAGAACCATGGCTGATGGAGATGCCATTTCCATCTTTGGTTCAAGCCATATATGGATTGACCACAATTCACTCTCTAATTGTGCTGACGGACTTGTTGATGCTGTTATGGGCTCAACTGCAATCACGATCTCCAACAATCACTTCACTCATCATAATGAGGTACACGTTTTATGGCATTTCTTATATGGAATTAAATTGAAAACACAAATTTATCTGATGGAATTGCATCTTTAACAGGTGATGCTTCTAGGCCACAGTGATTCCTACACGAAGGACAAGCAAATGCAAGTCACCATTGCTTATAACCATTTTGGAGAAGGACTCATTCAAAGAATGCCAAGGTAAATATAAATAGGTATCAATGGTTTGAATTGTGTTTTGAGTTTATTAACTGAGATTTTACCTCACATATTTTCAATAATGTATAGATGTAGGCATGGCTATTTCCATGTGGTGAACAATGACTACACTCACTGGGAGATGTATGCCATTGGTGGGAGTGCAAATCCCACCATCAATAGTCAAGGAAACAGATATGCTGCTCCAACCAACCGTTTTGCCAAAGAGGTACACTCTTCCCAAACTCATCTCAAGCGTAGATTTAGATTCTGATATTCGATATAGCAGTTGACAAATCATGTCGATTCAATACAATACAGGTGACAAAGCGAGTTGAAACACCAGAGAGCGAATGGAAGGGGTGGAATTGGAGATCAGAAGGAGATATGTTGCTGAATGGAGCCTATTTCACTCCATCAGGTGCTGGAGCTTCAGCAAGCTATGCCAGAGCTTCCAGCTTAGGAGCGAAATCTGCTTCCATGGTTGGCTCCATCACTTCTTCTGCAGGTTCACTCCCTTGTCGCCGAGGCCATCCATGCTAGGACTTGTGTCCTCCATCATTTTAACTTCAAACACCAAATGTTAAAAAAGCCATAGACAAAAAAAGCCTTTCCTCATCTTTCGGTTTGATTTTCAAAAAACTTCCATCATTTCTCATCCATCATCTCCTTTGTCCCTTCATTCAAATAGCCATTTTAACAAGTACACATATTTTTTCTTTTTTCAAACTTGTTTTTAAAACCTTCATTCCACCATTGTTGTTCATCTGTTGGTTGCTCTCAACCTCGGCCTGCTTCAGCTGAAAAGAAAATGCCATTTTAGGGGCAGGTGCAGAAGCGTTGTCTCTTTTTATTTTTGGGTCTGTTTATTTTACCATCAAGTTTAACTCATCTTTCCATCTCTTACCACAGTCATTTTGTGATGCAATATTGTTTGGTCACTACACTATGTTTTTACTCTGGGTTTTGTATTTTCTTTTTTCTTTTTCCCCTTCCGAGTCCATGAGAGAGACAACTTAAAGAAGATTACAAAAGTTAAAAAGGAAAGTAGTGATGTTGTTTGTTATTGTTTTTGTCAGTTTTATTGGCCTTTTGATTTTTTTTATTTTTTTTTCACTTAGTGGGTAATGTTTAAGCTTTGGTTACAGTTGGTTATGTTTATATACTCATTATTATAGCCTCCCAAATTGTTTGTGA

mRNA sequence

CTTTCACTGACATCACCACTTCTCGTTTTCCTCtcttcttcttcttcttcttcCCTTTCCTTTTTTCTCTCTATATTTCTTCTGCTTCCTATAATCTCTCTCTCTCTCTACATTTCGTGCTGTTTTTTCTTTTGACGAGAGAGAGAGAGATAGCAGTTATGGCGGCACTTACTAAATGGTCTTCTCTGTCTCTCTTTGCCCTTGCATTGCTGTTTTTCCTAACAATCGCTCTCGTTCGCAAAGAAGAGACCTCCAAAATTAGGACTGTGTCAGCGGCGGAGTTTCAGAGCTCCAGCAACTCATCAATGGCGACTAGGGTGGAGAATTATGATGTGGAGCAAGAACTCAACAATGAACATGCTGTGGATAATCCTGATGAGATTGCTGCTTCTGTTGAGATGAGCATCCGTAATAGCACTGAAAGGAGGAAGTTGGGATTCTTCTCCTGTGGAACAGGGAATCCCATTGACGACTGCTGGCGTTGTGACTCCAATTGGCACCGTAACCGTAAACGCCTTGCGGAATGTGGCATTGGATTTGGGCGGAATGCTATTGGTGGACGTGATGGCCGTTTCTATGTTGTCACTGATTCGAGTGACAACGATCCGGTGAACCCGAAGCCAGGGACTCTTCGTCATGCTGTTATTCAAGAGAAACCTCTTTGGATCGTGTTCAAAAGGGATATGGTGATTCGATTGAAACAGGAACTCATCATGAACAGTTTTAAGACAATTGATGCTCGAGGGGTAAATGTACACATTGCTAATGGCGCTTGCATCACAATCCAGTTTGTGACTAACATCATTATCCATGGTCTACACATCCATGATTGCAAACCCACAGGGAATGCCATGGTGAGAAGCTCTCCATCTCATTTTGGGTGGAGAACCATGGCTGATGGAGATGCCATTTCCATCTTTGGTTCAAGCCATATATGGATTGACCACAATTCACTCTCTAATTGTGCTGACGGACTTGTTGATGCTGTTATGGGCTCAACTGCAATCACGATCTCCAACAATCACTTCACTCATCATAATGAGGTGATGCTTCTAGGCCACAGTGATTCCTACACGAAGGACAAGCAAATGCAAGTCACCATTGCTTATAACCATTTTGGAGAAGGACTCATTCAAAGAATGCCAAGATGTAGGCATGGCTATTTCCATGTGGTGAACAATGACTACACTCACTGGGAGATGTATGCCATTGGTGGGAGTGCAAATCCCACCATCAATAGTCAAGGAAACAGATATGCTGCTCCAACCAACCGTTTTGCCAAAGAGGTGACAAAGCGAGTTGAAACACCAGAGAGCGAATGGAAGGGGTGGAATTGGAGATCAGAAGGAGATATGTTGCTGAATGGAGCCTATTTCACTCCATCAGGTGCTGGAGCTTCAGCAAGCTATGCCAGAGCTTCCAGCTTAGGAGCGAAATCTGCTTCCATGGTTGGCTCCATCACTTCTTCTGCAGGTTCACTCCCTTGTCGCCGAGGCCATCCATGCTAGGACTTGTGTCCTCCATCATTTTAACTTCAAACACCAAATGTTAAAAAAGCCATAGACAAAAAAAGCCTTTCCTCATCTTTCGGTTTGATTTTCAAAAAACTTCCATCATTTCTCATCCATCATCTCCTTTGTCCCTTCATTCAAATAGCCATTTTAACAAGTACACATATTTTTTCTTTTTTCAAACTTGTTTTTAAAACCTTCATTCCACCATTGTTGTTCATCTGTTGGTTGCTCTCAACCTCGGCCTGCTTCAGCTGAAAAGAAAATGCCATTTTAGGGGCAGGTGCAGAAGCGTTGTCTCTTTTTATTTTTGGGTCTGTTTATTTTACCATCAAGTTTAACTCATCTTTCCATCTCTTACCACAGTCATTTTGTGATGCAATATTGTTTGGTCACTACACTATGTTTTTACTCTGGGTTTTGTATTTTCTTTTTTCTTTTTCCCCTTCCGAGTCCATGAGAGAGACAACTTAAAGAAGATTACAAAAGTTAAAAAGGAAAGTAGTGATGTTGTTTGTTATTGTTTTTGTCAGTTTTATTGGCCTTTTGATTTTTTTTATTTTTTTTTCACTTAGTGGGTAATGTTTAAGCTTTGGTTACAGTTGGTTATGTTTATATACTCATTATTATAGCCTCCCAAATTGTTTGTGA

Coding sequence (CDS)

ATGGCGGCACTTACTAAATGGTCTTCTCTGTCTCTCTTTGCCCTTGCATTGCTGTTTTTCCTAACAATCGCTCTCGTTCGCAAAGAAGAGACCTCCAAAATTAGGACTGTGTCAGCGGCGGAGTTTCAGAGCTCCAGCAACTCATCAATGGCGACTAGGGTGGAGAATTATGATGTGGAGCAAGAACTCAACAATGAACATGCTGTGGATAATCCTGATGAGATTGCTGCTTCTGTTGAGATGAGCATCCGTAATAGCACTGAAAGGAGGAAGTTGGGATTCTTCTCCTGTGGAACAGGGAATCCCATTGACGACTGCTGGCGTTGTGACTCCAATTGGCACCGTAACCGTAAACGCCTTGCGGAATGTGGCATTGGATTTGGGCGGAATGCTATTGGTGGACGTGATGGCCGTTTCTATGTTGTCACTGATTCGAGTGACAACGATCCGGTGAACCCGAAGCCAGGGACTCTTCGTCATGCTGTTATTCAAGAGAAACCTCTTTGGATCGTGTTCAAAAGGGATATGGTGATTCGATTGAAACAGGAACTCATCATGAACAGTTTTAAGACAATTGATGCTCGAGGGGTAAATGTACACATTGCTAATGGCGCTTGCATCACAATCCAGTTTGTGACTAACATCATTATCCATGGTCTACACATCCATGATTGCAAACCCACAGGGAATGCCATGGTGAGAAGCTCTCCATCTCATTTTGGGTGGAGAACCATGGCTGATGGAGATGCCATTTCCATCTTTGGTTCAAGCCATATATGGATTGACCACAATTCACTCTCTAATTGTGCTGACGGACTTGTTGATGCTGTTATGGGCTCAACTGCAATCACGATCTCCAACAATCACTTCACTCATCATAATGAGGTGATGCTTCTAGGCCACAGTGATTCCTACACGAAGGACAAGCAAATGCAAGTCACCATTGCTTATAACCATTTTGGAGAAGGACTCATTCAAAGAATGCCAAGATGTAGGCATGGCTATTTCCATGTGGTGAACAATGACTACACTCACTGGGAGATGTATGCCATTGGTGGGAGTGCAAATCCCACCATCAATAGTCAAGGAAACAGATATGCTGCTCCAACCAACCGTTTTGCCAAAGAGGTGACAAAGCGAGTTGAAACACCAGAGAGCGAATGGAAGGGGTGGAATTGGAGATCAGAAGGAGATATGTTGCTGAATGGAGCCTATTTCACTCCATCAGGTGCTGGAGCTTCAGCAAGCTATGCCAGAGCTTCCAGCTTAGGAGCGAAATCTGCTTCCATGGTTGGCTCCATCACTTCTTCTGCAGGTTCACTCCCTTGTCGCCGAGGCCATCCATGCTAG

Protein sequence

MAALTKWSSLSLFALALLFFLTIALVRKEETSKIRTVSAAEFQSSSNSSMATRVENYDVEQELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRNRKRLAECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIRLKQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPSHFGWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLGHSDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGNRYAAPTNRFAKEVTKRVETPESEWKGWNWRSEGDMLLNGAYFTPSGAGASASYARASSLGAKSASMVGSITSSAGSLPCRRGHPC*

Homology

[Close]

BLAST of Cucsa.194310.1 vs. Swiss-Prot
Match: PLY15_ARATH (Probable pectate lyase 15 OS=Arabidopsis thaliana GN=At4g13710 PE=2 SV=1)

HSP 1 Score: 725.7 bits (1872), Expect = 3.1e-208
Identity = 357/459 (77.78%), Postives = 393/459 (85.62%), Query Frame = 1


Query: 10  LSLFALALLFFLT--IALVRKEET-----------SKIRTVSAAEFQSSSNSSMA-TRVE 69
           L + AL  + F    I+L RK +T           + IR +   EFQS ++S+MA TR++
Sbjct: 16  LVVLALTAMIFRNSEISLSRKLKTEVIQSSNSSTMAAIRKLKTEEFQSLNSSTMAATRLD 75

Query: 70  NYDVEQELNNEHAV-DNPD----EIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCD 129
               +Q+    HAV D+PD    E+A  V+MS +N T RRKLGFFSCGTGNPIDDCWRCD
Sbjct: 76  GEPQQQQ----HAVADDPDMVADEVAKLVQMSEQNRTARRKLGFFSCGTGNPIDDCWRCD 135

Query: 130 SNWHRNRKRLAECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWI 189
            NWH+NRKRLA+CGIGFGRNAIGGRDGRFY+VTD +D D VNPKPGTLRHAVIQE+PLWI
Sbjct: 136 RNWHKNRKRLADCGIGFGRNAIGGRDGRFYIVTDPTDEDVVNPKPGTLRHAVIQEEPLWI 195

Query: 190 VFKRDMVIRLKQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGN 249
           VFKRDMVI LKQELIMNSFKTIDARG NVHIANGACITIQF+TN+IIHGLHIHDCKPTGN
Sbjct: 196 VFKRDMVIELKQELIMNSFKTIDARGSNVHIANGACITIQFITNVIIHGLHIHDCKPTGN 255

Query: 250 AMVRSSPSHFGWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHF 309
           AMVRSSPSHFGWRTMADGDA+SIFGSSHIWIDHNSLS+CADGLVDAVMGSTAIT+SNNHF
Sbjct: 256 AMVRSSPSHFGWRTMADGDAVSIFGSSHIWIDHNSLSHCADGLVDAVMGSTAITVSNNHF 315

Query: 310 THHNEVMLLGHSDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYA 369
           THHNEVMLLGHSDSYTKDK MQVTIAYNHFGEGL+QRMPRCRHGYFHVVNNDYTHWEMYA
Sbjct: 316 THHNEVMLLGHSDSYTKDKLMQVTIAYNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMYA 375

Query: 370 IGGSANPTINSQGNRYAAPTNRFAKEVTKRVETPESEWKGWNWRSEGDMLLNGAYFTPSG 429
           IGGSA PTINSQGNRYAAP +RFAKEVTKRVET  SEWK WNWRSEGD+LLNGA+F PSG
Sbjct: 376 IGGSAEPTINSQGNRYAAPMDRFAKEVTKRVETDASEWKKWNWRSEGDLLLNGAFFRPSG 435

Query: 430 AGASASYARASSLGAKSASMVGSITSSAGSLPCRRGHPC 450
           AGASASY RASSL AK +SMV +ITS+AG+L CR+G PC
Sbjct: 436 AGASASYGRASSLAAKPSSMVDTITSTAGALGCRKGRPC 470

[Close]

BLAST of Cucsa.194310.1 vs. Swiss-Prot
Match: PLY1_ARATH (Probable pectate lyase 1 OS=Arabidopsis thaliana GN=At1g04680 PE=2 SV=2)

HSP 1 Score: 714.9 bits (1844), Expect = 5.5e-205
Identity = 347/453 (76.60%), Postives = 376/453 (83.00%), Query Frame = 1


Query: 1   MAALTKWSSLSLFALALLFFLTIALVRKEETSKIRTVSAAEFQSSSNSSMATRVENYDVE 60
           MA L  W    L  + LLFF                V A E  +  N S   R +  +  
Sbjct: 1   MAVLPTWL---LAMMCLLFF----------------VGAMENTTHDNISSLPRSDETEW- 60

Query: 61  QELNNEHAVDNPDEIAASV----EMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRN 120
               N+HAV NPDE+A  V    EMS+RN TERRKLG+F+CGTGNPIDDCWRCD NWH+N
Sbjct: 61  ----NQHAVTNPDEVADEVLALTEMSVRNHTERRKLGYFTCGTGNPIDDCWRCDPNWHKN 120

Query: 121 RKRLAECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDM 180
           RKRLA+CGIGFGRNAIGGRDGRFYVVTD  D++PVNP+PGTLRHAVIQ++PLWIVFKRDM
Sbjct: 121 RKRLADCGIGFGRNAIGGRDGRFYVVTDPRDDNPVNPRPGTLRHAVIQDRPLWIVFKRDM 180

Query: 181 VIRLKQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSS 240
           VI+LKQELI+NSFKTID RG NVHIANG CITIQFVTN+I+HGLHIHDCKPTGNAMVRSS
Sbjct: 181 VIQLKQELIVNSFKTIDGRGANVHIANGGCITIQFVTNVIVHGLHIHDCKPTGNAMVRSS 240

Query: 241 PSHFGWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEV 300
            +HFGWRTMADGDAISIFGSSH+WIDHNSLS+CADGLVDAVMGSTAITISNNH THHNEV
Sbjct: 241 ETHFGWRTMADGDAISIFGSSHVWIDHNSLSHCADGLVDAVMGSTAITISNNHLTHHNEV 300

Query: 301 MLLGHSDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSAN 360
           MLLGHSDSY +DK MQVTIAYNHFG GLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSAN
Sbjct: 301 MLLGHSDSYMRDKAMQVTIAYNHFGVGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSAN 360

Query: 361 PTINSQGNRYAAPTNRFAKEVTKRVETPESEWKGWNWRSEGDMLLNGAYFTPSGAGASAS 420
           PTINSQGNRYAAP N FAKEVTKRV+TP S WKGWNWRSEGD+L NGAYFT SGA AS S
Sbjct: 361 PTINSQGNRYAAPKNPFAKEVTKRVDTPASHWKGWNWRSEGDLLQNGAYFTSSGAAASGS 420

Query: 421 YARASSLGAKSASMVGSITSSAGSLPCRRGHPC 450
           YARASSL AKS+S+VG ITS AG+LPCRRG  C
Sbjct: 421 YARASSLSAKSSSLVGHITSDAGALPCRRGRQC 429

[Close]

BLAST of Cucsa.194310.1 vs. Swiss-Prot
Match: PLY8_ARATH (Probable pectate lyase 8 OS=Arabidopsis thaliana GN=At3g07010 PE=2 SV=1)

HSP 1 Score: 690.3 bits (1780), Expect = 1.4e-197
Identity = 322/394 (81.73%), Postives = 354/394 (89.85%), Query Frame = 1


Query: 56  NYDVEQELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHR 115
           N     E  +EHAV+NPDE+AA V+MSIRNSTERR+LG+FSC TGNPIDDCWRCD  W  
Sbjct: 23  NASRSNETWHEHAVENPDEVAAMVDMSIRNSTERRRLGYFSCATGNPIDDCWRCDRKWQL 82

Query: 116 NRKRLAECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRD 175
            RKRLA+C IGFGRNAIGGRDGRFYVVTD  D+DPVNP PGTLRHAVIQ++PLWI+FKRD
Sbjct: 83  RRKRLADCSIGFGRNAIGGRDGRFYVVTDPGDDDPVNPIPGTLRHAVIQDEPLWIIFKRD 142

Query: 176 MVIRLKQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRS 235
           MVI LKQELIMNSFKTID RGVNVHIANGAC+TIQ+VTNII+HG+H+HDCKPTGNAMVRS
Sbjct: 143 MVITLKQELIMNSFKTIDGRGVNVHIANGACLTIQYVTNIIVHGIHVHDCKPTGNAMVRS 202

Query: 236 SPSHFGWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNE 295
           SPSH+G+R+MADGDAISIFGSSHIWIDHNSLSNCADGLVDAVM STAIT+SNN FTHHNE
Sbjct: 203 SPSHYGFRSMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMSSTAITVSNNFFTHHNE 262

Query: 296 VMLLGHSDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSA 355
           VMLLGHSDSYT+DK MQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSA
Sbjct: 263 VMLLGHSDSYTRDKVMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSA 322

Query: 356 NPTINSQGNRYAAPTNRFAKEVTKRVETPESEWKGWNWRSEGDMLLNGAYFTPSGAGASA 415
            PTINSQGNR+ AP N FAKEVTKR  T ES+WK WNWRSEGD+ LNGA+FT SGAGA A
Sbjct: 323 GPTINSQGNRFLAPVNPFAKEVTKREYTGESKWKHWNWRSEGDLFLNGAFFTRSGAGAGA 382

Query: 416 SYARASSLGAKSASMVGSITSSAGSLPCRRGHPC 450
           +YARASSL AKS+S+VG++TS +G+L CR G  C
Sbjct: 383 NYARASSLSAKSSSLVGTMTSYSGALNCRAGRRC 416

[Close]

BLAST of Cucsa.194310.1 vs. Swiss-Prot
Match: PLY10_ARATH (Probable pectate lyase 10 OS=Arabidopsis thaliana GN=At3g24670 PE=2 SV=2)

HSP 1 Score: 686.0 bits (1769), Expect = 2.7e-196
Identity = 330/435 (75.86%), Postives = 367/435 (84.37%), Query Frame = 1


Query: 15  LALLFFLTIALVRKEETSKIRTVSAAEFQSSSNSSMATRVENYDVEQELNNEHAVDNPDE 74
           LAL   L I  +    ++  +        SSSNSS A ++ N D      NEHAV NP+E
Sbjct: 9   LALSTTLIILALCINSSTMAQETEDLNSHSSSNSSTANKLPNDDGAW---NEHAVKNPEE 68

Query: 75  IAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRNRKRLAECGIGFGRNAIGG 134
           +AA V+M I+NSTERR+LGFFSC TGNPIDDCWRCD NWH  RKRLA C IGFGRNAIGG
Sbjct: 69  VAAMVDMKIKNSTERRRLGFFSCATGNPIDDCWRCDRNWHLRRKRLANCAIGFGRNAIGG 128

Query: 135 RDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIRLKQELIMNSFKTIDA 194
           RDGR+YVVTD SD+D VNP+PGTLRHAVIQ++PLWIVFKRDMVI L QELIMNSFKTID 
Sbjct: 129 RDGRYYVVTDPSDHDAVNPRPGTLRHAVIQDRPLWIVFKRDMVITLTQELIMNSFKTIDG 188

Query: 195 RGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPSHFGWRTMADGDAISIF 254
           RGVNV IA GACITIQ+VTNIIIHG+++HDC+ TGNAMVRSSPSH+GWRTMADGDAISIF
Sbjct: 189 RGVNVAIAGGACITIQYVTNIIIHGINVHDCRRTGNAMVRSSPSHYGWRTMADGDAISIF 248

Query: 255 GSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLGHSDSYTKDKQMQVT 314
           GSSHIWIDHNSLSNCADGL+DA+MGSTAITISNN+ THHNEVML+GHSDSYT+DK MQVT
Sbjct: 249 GSSHIWIDHNSLSNCADGLIDAIMGSTAITISNNYMTHHNEVMLMGHSDSYTRDKLMQVT 308

Query: 315 IAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGNRYAAPTNRFA 374
           IAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHW MYAIGGSANPTINSQGNR+ AP N FA
Sbjct: 309 IAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWVMYAIGGSANPTINSQGNRFLAPGNPFA 368

Query: 375 KEVTKRVETPESEWKGWNWRSEGDMLLNGAYFTPSGAGASASYARASSLGAKSASMVGSI 434
           KEVTKRV + + EWK WNWRS+GD++LNGAYFT SGA A ASYARASSLGAK AS+V  +
Sbjct: 369 KEVTKRVGSWQGEWKQWNWRSQGDLMLNGAYFTKSGAAAPASYARASSLGAKPASVVSML 428

Query: 435 TSSAGSLPCRRGHPC 450
           T S+G+L CR G  C
Sbjct: 429 TYSSGALKCRIGMRC 440

[Close]

BLAST of Cucsa.194310.1 vs. Swiss-Prot
Match: PLY20_ARATH (Probable pectate lyase 20 OS=Arabidopsis thaliana GN=At5g48900 PE=2 SV=1)

HSP 1 Score: 683.3 bits (1762), Expect = 1.8e-195
Identity = 317/385 (82.34%), Postives = 349/385 (90.65%), Query Frame = 1


Query: 65  NEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRNRKRLAECG 124
           +EHAV+NP+E+AA V+MSIRNST RR+LG+FSC TGNPIDDCWRCD  W   RK LA C 
Sbjct: 33  HEHAVENPEEVAAMVDMSIRNSTARRRLGYFSCSTGNPIDDCWRCDRRWQSRRKHLANCA 92

Query: 125 IGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIRLKQEL 184
           IGFGRNAIGGRDGR+YVV+D +D++PVNPKPGTLRHAVIQE+PLWIVFKRDMVI LK+EL
Sbjct: 93  IGFGRNAIGGRDGRYYVVSDPNDDNPVNPKPGTLRHAVIQEEPLWIVFKRDMVITLKEEL 152

Query: 185 IMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPSHFGWRT 244
           IMNSFKTID RGVNVHIANGACITIQFVTNIIIHG+HIHDC+PTGNAMVRSSPSH+GWRT
Sbjct: 153 IMNSFKTIDGRGVNVHIANGACITIQFVTNIIIHGIHIHDCRPTGNAMVRSSPSHYGWRT 212

Query: 245 MADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLGHSDS 304
           MADGD ISIFGSSHIWIDHNSLSNCADGL+DAVM STAITISNN+FTHHNEVMLLGHSD+
Sbjct: 213 MADGDGISIFGSSHIWIDHNSLSNCADGLIDAVMASTAITISNNYFTHHNEVMLLGHSDT 272

Query: 305 YTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGN 364
           YT+DK MQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSA+PTINSQGN
Sbjct: 273 YTRDKVMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSASPTINSQGN 332

Query: 365 RYAAPTNRFAKEVTKRVETPESEWKGWNWRSEGDMLLNGAYFTPSGAGASASYARASSLG 424
           RY AP NRFAKEVTKR    + +W+ WNWRSEGD+ LNGA+FT SG+G  ASYARASSL 
Sbjct: 333 RYLAPRNRFAKEVTKRDYAGQWQWRHWNWRSEGDLFLNGAFFTRSGSGLGASYARASSLA 392

Query: 425 AKSASMVGSITSSAGSLPCRRGHPC 450
           AKS+S+VG IT +AG+L CR G  C
Sbjct: 393 AKSSSLVGVITYNAGALNCRGGRRC 417

[Close]

BLAST of Cucsa.194310.1 vs. TrEMBL
Match: A0A0A0LI24_CUCSA (Pectate lyase OS=Cucumis sativus GN=Csa_2G155600 PE=3 SV=1)

HSP 1 Score: 927.2 bits (2395), Expect = 7.8e-267
Identity = 449/449 (100.00%), Postives = 449/449 (100.00%), Query Frame = 1


Query: 1   MAALTKWSSLSLFALALLFFLTIALVRKEETSKIRTVSAAEFQSSSNSSMATRVENYDVE 60
           MAALTKWSSLSLFALALLFFLTIALVRKEETSKIRTVSAAEFQSSSNSSMATRVENYDVE
Sbjct: 1   MAALTKWSSLSLFALALLFFLTIALVRKEETSKIRTVSAAEFQSSSNSSMATRVENYDVE 60

Query: 61  QELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRNRKRL 120
           QELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRNRKRL
Sbjct: 61  QELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRNRKRL 120

Query: 121 AECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIRL 180
           AECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIRL
Sbjct: 121 AECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIRL 180

Query: 181 KQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPSHF 240
           KQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPSHF
Sbjct: 181 KQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPSHF 240

Query: 241 GWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLG 300
           GWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLG
Sbjct: 241 GWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLG 300

Query: 301 HSDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTIN 360
           HSDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTIN
Sbjct: 301 HSDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTIN 360

Query: 361 SQGNRYAAPTNRFAKEVTKRVETPESEWKGWNWRSEGDMLLNGAYFTPSGAGASASYARA 420
           SQGNRYAAPTNRFAKEVTKRVETPESEWKGWNWRSEGDMLLNGAYFTPSGAGASASYARA
Sbjct: 361 SQGNRYAAPTNRFAKEVTKRVETPESEWKGWNWRSEGDMLLNGAYFTPSGAGASASYARA 420

Query: 421 SSLGAKSASMVGSITSSAGSLPCRRGHPC 450
           SSLGAKSASMVGSITSSAGSLPCRRGHPC
Sbjct: 421 SSLGAKSASMVGSITSSAGSLPCRRGHPC 449

[Close]

BLAST of Cucsa.194310.1 vs. TrEMBL
Match: D4NXR8_PRUPE (Pectate lyase OS=Prunus persica GN=PRUPE_ppa005761mg PE=2 SV=1)

HSP 1 Score: 776.5 bits (2004), Expect = 1.7e-221
Identity = 370/444 (83.33%), Postives = 399/444 (89.86%), Query Frame = 1


Query: 6   KWSSLSLFALALLFFLTIALVRKEETSKIRTVSAAEFQSSSNSSMATRVENYDVEQELNN 65
           +W + +  A+ L+     A+    E S  R     E QSSSNSSMA RV   D   E  N
Sbjct: 8   RWVTGAFLAVLLVLCFVAAIA---EISGNRNGGTEELQSSSNSSMAARVAEDD---ESFN 67

Query: 66  EHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRNRKRLAECGI 125
           +HAVD+P+E+ A V+MSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNW +NRKRLA+CGI
Sbjct: 68  KHAVDDPEEVVAMVDMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWQKNRKRLADCGI 127

Query: 126 GFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIRLKQELI 185
           GFGRNAIGGRDGRFYVVTD  D+DPVNP+PGTLRHAVIQ +PLWIVFKRDMVI+LKQELI
Sbjct: 128 GFGRNAIGGRDGRFYVVTDPGDDDPVNPRPGTLRHAVIQNEPLWIVFKRDMVIQLKQELI 187

Query: 186 MNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPSHFGWRTM 245
           MNSFKTID RGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNA+VRSSPSHFGWRTM
Sbjct: 188 MNSFKTIDGRGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNALVRSSPSHFGWRTM 247

Query: 246 ADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLGHSDSY 305
           ADGDA+SIFGSSHIW+DHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLGHSDSY
Sbjct: 248 ADGDAVSIFGSSHIWVDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLGHSDSY 307

Query: 306 TKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGNR 365
           T+DK MQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSA PTINSQGNR
Sbjct: 308 TRDKAMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSAEPTINSQGNR 367

Query: 366 YAAPTNRFAKEVTKRVETPESEWKGWNWRSEGDMLLNGAYFTPSGAGASASYARASSLGA 425
           YAAPTN FAKEVTKRVETP ++WK WNWRSEGD+LLNGAYFTPSGAGASASYARASSLGA
Sbjct: 368 YAAPTNPFAKEVTKRVETPTTQWKSWNWRSEGDLLLNGAYFTPSGAGASASYARASSLGA 427

Query: 426 KSASMVGSITSSAGSLPCRRGHPC 450
           KS++MVG+ITS +G+LPCRRGHPC
Sbjct: 428 KSSAMVGAITSGSGALPCRRGHPC 445

[Close]

BLAST of Cucsa.194310.1 vs. TrEMBL
Match: A1IIA8_PRUPE (Pectate lyase (Fragment) OS=Prunus persica GN=PpPL2 PE=2 SV=1)

HSP 1 Score: 773.9 bits (1997), Expect = 1.1e-220
Identity = 366/420 (87.14%), Postives = 389/420 (92.62%), Query Frame = 1


Query: 30  ETSKIRTVSAAEFQSSSNSSMATRVENYDVEQELNNEHAVDNPDEIAASVEMSIRNSTER 89
           E S  R     E QSSSNSSMA RV   D   E  N+HAVD+P+E+ A V+MSIRNSTER
Sbjct: 9   EISGNRNGGTEELQSSSNSSMAARVAEDD---ESFNKHAVDDPEEVVAMVDMSIRNSTER 68

Query: 90  RKLGFFSCGTGNPIDDCWRCDSNWHRNRKRLAECGIGFGRNAIGGRDGRFYVVTDSSDND 149
           RKLGFFSCGTGNPIDDCWRCDSNW +NRKRLA+CGIGFGRNAIGGRDGRFYVVTD  D+D
Sbjct: 69  RKLGFFSCGTGNPIDDCWRCDSNWQKNRKRLADCGIGFGRNAIGGRDGRFYVVTDPGDDD 128

Query: 150 PVNPKPGTLRHAVIQEKPLWIVFKRDMVIRLKQELIMNSFKTIDARGVNVHIANGACITI 209
           PVNP+PGTLRHAVIQ +PLWIVFKRDMVI+LKQELIMNSFKTID RGVNVHIANGACITI
Sbjct: 129 PVNPRPGTLRHAVIQNEPLWIVFKRDMVIQLKQELIMNSFKTIDGRGVNVHIANGACITI 188

Query: 210 QFVTNIIIHGLHIHDCKPTGNAMVRSSPSHFGWRTMADGDAISIFGSSHIWIDHNSLSNC 269
           QFVTNIIIHGLHIHDCKPTGNA+VRSSPSHFGWRTMADGDA+SIFGSSHIW+DHNSLSNC
Sbjct: 189 QFVTNIIIHGLHIHDCKPTGNALVRSSPSHFGWRTMADGDAVSIFGSSHIWVDHNSLSNC 248

Query: 270 ADGLVDAVMGSTAITISNNHFTHHNEVMLLGHSDSYTKDKQMQVTIAYNHFGEGLIQRMP 329
           ADGLVDAVMGSTAITISNNHFTHHNEVMLLGHSDSYT+DK MQVTIAYNHFGEGLIQRMP
Sbjct: 249 ADGLVDAVMGSTAITISNNHFTHHNEVMLLGHSDSYTRDKAMQVTIAYNHFGEGLIQRMP 308

Query: 330 RCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGNRYAAPTNRFAKEVTKRVETPESEWK 389
           RCRHGYFHVVNNDYTHWEMYAIGGSA PTINSQGNRYAAPTN FAKEVTKRVETP ++WK
Sbjct: 309 RCRHGYFHVVNNDYTHWEMYAIGGSAEPTINSQGNRYAAPTNPFAKEVTKRVETPTTQWK 368

Query: 390 GWNWRSEGDMLLNGAYFTPSGAGASASYARASSLGAKSASMVGSITSSAGSLPCRRGHPC 449
            WNWRSEGD+LLNGAYFTPSGAGASASYARASSLGAKS++MVG+ITS +G+LPCRRGHPC
Sbjct: 369 SWNWRSEGDLLLNGAYFTPSGAGASASYARASSLGAKSSAMVGAITSGSGALPCRRGHPC 425

[Close]

BLAST of Cucsa.194310.1 vs. TrEMBL
Match: A0A0A0L814_CUCSA (Pectate lyase OS=Cucumis sativus GN=Csa_3G133180 PE=3 SV=1)

HSP 1 Score: 768.5 bits (1983), Expect = 4.6e-219
Identity = 369/449 (82.18%), Postives = 403/449 (89.76%), Query Frame = 1


Query: 1   MAALTKWSSLSLFALALLFFLTIALVRKEETSKIRTVSAAEFQSSSNSSMATRVENYDVE 60
           MA   KW  L L  + +LF   IA   KE   +I      + QSSSNS+MA R+   D  
Sbjct: 3   MAESGKWIRLILLVVFMLFVGAIARAPKE-IPEIENGGTEKLQSSSNSTMAVRL---DEA 62

Query: 61  QELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRNRKRL 120
           ++L NEHAVDNP+EIAA V+MSIRN+T RR+LGFFSCGTGNPIDDCWRCD NW +NRKRL
Sbjct: 63  KDLGNEHAVDNPEEIAAMVDMSIRNATARRELGFFSCGTGNPIDDCWRCDPNWQQNRKRL 122

Query: 121 AECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIRL 180
           A+CGIGFGRNAIGGRDGRFYVVTDS D++PVNPKPGTLRHAVIQ++PLWIVFKRDMVI+L
Sbjct: 123 ADCGIGFGRNAIGGRDGRFYVVTDSGDDNPVNPKPGTLRHAVIQDEPLWIVFKRDMVIQL 182

Query: 181 KQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPSHF 240
           KQELIMNSFKTIDARG NVHIANGAC+TIQFVTN+I+HGL+IHDCKPTGNAMVRSSP+H 
Sbjct: 183 KQELIMNSFKTIDARGTNVHIANGACLTIQFVTNVIVHGLNIHDCKPTGNAMVRSSPNHV 242

Query: 241 GWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLG 300
           GWRT+ADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNN+FTHHNEVMLLG
Sbjct: 243 GWRTIADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNYFTHHNEVMLLG 302

Query: 301 HSDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTIN 360
           HSDSY +DK MQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTIN
Sbjct: 303 HSDSYVRDKLMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTIN 362

Query: 361 SQGNRYAAPTNRFAKEVTKRVETPESEWKGWNWRSEGDMLLNGAYFTPSGAGASASYARA 420
           SQGNRYAAPTN FAKEVTKRVET ESEWKGWNWRSEGD+LLNGA+F PSGAGAS+SYARA
Sbjct: 363 SQGNRYAAPTNPFAKEVTKRVETSESEWKGWNWRSEGDLLLNGAFFIPSGAGASSSYARA 422

Query: 421 SSLGAKSASMVGSITSSAGSLPCRRGHPC 450
           SSLGAKS+SMVG+ITS+AG+L CRRGH C
Sbjct: 423 SSLGAKSSSMVGTITSNAGALSCRRGHAC 447

[Close]

BLAST of Cucsa.194310.1 vs. TrEMBL
Match: A0A0D2S7U3_GOSRA (Pectate lyase OS=Gossypium raimondii GN=B456_006G219800 PE=3 SV=1)

HSP 1 Score: 765.8 bits (1976), Expect = 3.0e-218
Identity = 371/453 (81.90%), Postives = 398/453 (87.86%), Query Frame = 1


Query: 1   MAALTKWSSLSLFALALLFFLTIALVRKEETSKIRTVSAAEFQSSSNSSMATRVENYDVE 60
           MA    W+  SL  L LL    +A VR    S    V   + QSS NS+MA R E    E
Sbjct: 1   MAVSNNWAFASLALLFLLLVAAMATVRTGNVSS--NVETEKVQSSKNSTMAARSE----E 60

Query: 61  QELNNEHAVDNPDEIAASVE----MSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRN 120
            E  NEHAV +P+ +AA VE    M+IRN TERRKLGFF+CGTGNPIDDCWRCD NW +N
Sbjct: 61  VEPLNEHAVPDPEAVAAEVESIIDMNIRNVTERRKLGFFTCGTGNPIDDCWRCDPNWQKN 120

Query: 121 RKRLAECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDM 180
           RKRLA+CGIGFGRNAIGGRDGRFYVVTD +D+DPVNPKPGTLRHAVIQ++PLWIVFKRDM
Sbjct: 121 RKRLADCGIGFGRNAIGGRDGRFYVVTDPNDDDPVNPKPGTLRHAVIQDEPLWIVFKRDM 180

Query: 181 VIRLKQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSS 240
           VIRLKQELIMNSFKTID RGVNVHIANGACITIQ+VTN+IIHGLHIHDCKPTGNAMVRSS
Sbjct: 181 VIRLKQELIMNSFKTIDGRGVNVHIANGACITIQYVTNVIIHGLHIHDCKPTGNAMVRSS 240

Query: 241 PSHFGWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEV 300
           PSHFGWRTMADGDAISIFGSSHIW+DHNSLSNCADGLVDAVMGSTAITISNNH THHNEV
Sbjct: 241 PSHFGWRTMADGDAISIFGSSHIWVDHNSLSNCADGLVDAVMGSTAITISNNHMTHHNEV 300

Query: 301 MLLGHSDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSAN 360
           MLLGHSDSYT+DKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSAN
Sbjct: 301 MLLGHSDSYTRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSAN 360

Query: 361 PTINSQGNRYAAPTNRFAKEVTKRVETPESEWKGWNWRSEGDMLLNGAYFTPSGAGASAS 420
           PTINSQGNRYAAPTN FAKEVTKRV+T E +WK WNWRSEGD+LLNGAYFTPSGAGASAS
Sbjct: 361 PTINSQGNRYAAPTNPFAKEVTKRVDTAEGQWKSWNWRSEGDLLLNGAYFTPSGAGASAS 420

Query: 421 YARASSLGAKSASMVGSITSSAGSLPCRRGHPC 450
           YARASSLGAKS+SMVG++TS+AG+LPCRRG  C
Sbjct: 421 YARASSLGAKSSSMVGAMTSNAGALPCRRGKQC 447

[Close]

BLAST of Cucsa.194310.1 vs. TAIR10
Match: AT4G13710.1 (AT4G13710.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 725.7 bits (1872), Expect = 1.7e-209
Identity = 357/459 (77.78%), Postives = 393/459 (85.62%), Query Frame = 1


Query: 10  LSLFALALLFFLT--IALVRKEET-----------SKIRTVSAAEFQSSSNSSMA-TRVE 69
           L + AL  + F    I+L RK +T           + IR +   EFQS ++S+MA TR++
Sbjct: 16  LVVLALTAMIFRNSEISLSRKLKTEVIQSSNSSTMAAIRKLKTEEFQSLNSSTMAATRLD 75

Query: 70  NYDVEQELNNEHAV-DNPD----EIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCD 129
               +Q+    HAV D+PD    E+A  V+MS +N T RRKLGFFSCGTGNPIDDCWRCD
Sbjct: 76  GEPQQQQ----HAVADDPDMVADEVAKLVQMSEQNRTARRKLGFFSCGTGNPIDDCWRCD 135

Query: 130 SNWHRNRKRLAECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWI 189
            NWH+NRKRLA+CGIGFGRNAIGGRDGRFY+VTD +D D VNPKPGTLRHAVIQE+PLWI
Sbjct: 136 RNWHKNRKRLADCGIGFGRNAIGGRDGRFYIVTDPTDEDVVNPKPGTLRHAVIQEEPLWI 195

Query: 190 VFKRDMVIRLKQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGN 249
           VFKRDMVI LKQELIMNSFKTIDARG NVHIANGACITIQF+TN+IIHGLHIHDCKPTGN
Sbjct: 196 VFKRDMVIELKQELIMNSFKTIDARGSNVHIANGACITIQFITNVIIHGLHIHDCKPTGN 255

Query: 250 AMVRSSPSHFGWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHF 309
           AMVRSSPSHFGWRTMADGDA+SIFGSSHIWIDHNSLS+CADGLVDAVMGSTAIT+SNNHF
Sbjct: 256 AMVRSSPSHFGWRTMADGDAVSIFGSSHIWIDHNSLSHCADGLVDAVMGSTAITVSNNHF 315

Query: 310 THHNEVMLLGHSDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYA 369
           THHNEVMLLGHSDSYTKDK MQVTIAYNHFGEGL+QRMPRCRHGYFHVVNNDYTHWEMYA
Sbjct: 316 THHNEVMLLGHSDSYTKDKLMQVTIAYNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMYA 375

Query: 370 IGGSANPTINSQGNRYAAPTNRFAKEVTKRVETPESEWKGWNWRSEGDMLLNGAYFTPSG 429
           IGGSA PTINSQGNRYAAP +RFAKEVTKRVET  SEWK WNWRSEGD+LLNGA+F PSG
Sbjct: 376 IGGSAEPTINSQGNRYAAPMDRFAKEVTKRVETDASEWKKWNWRSEGDLLLNGAFFRPSG 435

Query: 430 AGASASYARASSLGAKSASMVGSITSSAGSLPCRRGHPC 450
           AGASASY RASSL AK +SMV +ITS+AG+L CR+G PC
Sbjct: 436 AGASASYGRASSLAAKPSSMVDTITSTAGALGCRKGRPC 470

[Close]

BLAST of Cucsa.194310.1 vs. TAIR10
Match: AT1G04680.1 (AT1G04680.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 714.9 bits (1844), Expect = 3.1e-206
Identity = 347/453 (76.60%), Postives = 376/453 (83.00%), Query Frame = 1


Query: 1   MAALTKWSSLSLFALALLFFLTIALVRKEETSKIRTVSAAEFQSSSNSSMATRVENYDVE 60
           MA L  W    L  + LLFF                V A E  +  N S   R +  +  
Sbjct: 1   MAVLPTWL---LAMMCLLFF----------------VGAMENTTHDNISSLPRSDETEW- 60

Query: 61  QELNNEHAVDNPDEIAASV----EMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRN 120
               N+HAV NPDE+A  V    EMS+RN TERRKLG+F+CGTGNPIDDCWRCD NWH+N
Sbjct: 61  ----NQHAVTNPDEVADEVLALTEMSVRNHTERRKLGYFTCGTGNPIDDCWRCDPNWHKN 120

Query: 121 RKRLAECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDM 180
           RKRLA+CGIGFGRNAIGGRDGRFYVVTD  D++PVNP+PGTLRHAVIQ++PLWIVFKRDM
Sbjct: 121 RKRLADCGIGFGRNAIGGRDGRFYVVTDPRDDNPVNPRPGTLRHAVIQDRPLWIVFKRDM 180

Query: 181 VIRLKQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSS 240
           VI+LKQELI+NSFKTID RG NVHIANG CITIQFVTN+I+HGLHIHDCKPTGNAMVRSS
Sbjct: 181 VIQLKQELIVNSFKTIDGRGANVHIANGGCITIQFVTNVIVHGLHIHDCKPTGNAMVRSS 240

Query: 241 PSHFGWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEV 300
            +HFGWRTMADGDAISIFGSSH+WIDHNSLS+CADGLVDAVMGSTAITISNNH THHNEV
Sbjct: 241 ETHFGWRTMADGDAISIFGSSHVWIDHNSLSHCADGLVDAVMGSTAITISNNHLTHHNEV 300

Query: 301 MLLGHSDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSAN 360
           MLLGHSDSY +DK MQVTIAYNHFG GLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSAN
Sbjct: 301 MLLGHSDSYMRDKAMQVTIAYNHFGVGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSAN 360

Query: 361 PTINSQGNRYAAPTNRFAKEVTKRVETPESEWKGWNWRSEGDMLLNGAYFTPSGAGASAS 420
           PTINSQGNRYAAP N FAKEVTKRV+TP S WKGWNWRSEGD+L NGAYFT SGA AS S
Sbjct: 361 PTINSQGNRYAAPKNPFAKEVTKRVDTPASHWKGWNWRSEGDLLQNGAYFTSSGAAASGS 420

Query: 421 YARASSLGAKSASMVGSITSSAGSLPCRRGHPC 450
           YARASSL AKS+S+VG ITS AG+LPCRRG  C
Sbjct: 421 YARASSLSAKSSSLVGHITSDAGALPCRRGRQC 429

[Close]

BLAST of Cucsa.194310.1 vs. TAIR10
Match: AT3G07010.1 (AT3G07010.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 690.3 bits (1780), Expect = 8.1e-199
Identity = 322/394 (81.73%), Postives = 354/394 (89.85%), Query Frame = 1


Query: 56  NYDVEQELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHR 115
           N     E  +EHAV+NPDE+AA V+MSIRNSTERR+LG+FSC TGNPIDDCWRCD  W  
Sbjct: 23  NASRSNETWHEHAVENPDEVAAMVDMSIRNSTERRRLGYFSCATGNPIDDCWRCDRKWQL 82

Query: 116 NRKRLAECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRD 175
            RKRLA+C IGFGRNAIGGRDGRFYVVTD  D+DPVNP PGTLRHAVIQ++PLWI+FKRD
Sbjct: 83  RRKRLADCSIGFGRNAIGGRDGRFYVVTDPGDDDPVNPIPGTLRHAVIQDEPLWIIFKRD 142

Query: 176 MVIRLKQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRS 235
           MVI LKQELIMNSFKTID RGVNVHIANGAC+TIQ+VTNII+HG+H+HDCKPTGNAMVRS
Sbjct: 143 MVITLKQELIMNSFKTIDGRGVNVHIANGACLTIQYVTNIIVHGIHVHDCKPTGNAMVRS 202

Query: 236 SPSHFGWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNE 295
           SPSH+G+R+MADGDAISIFGSSHIWIDHNSLSNCADGLVDAVM STAIT+SNN FTHHNE
Sbjct: 203 SPSHYGFRSMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMSSTAITVSNNFFTHHNE 262

Query: 296 VMLLGHSDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSA 355
           VMLLGHSDSYT+DK MQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSA
Sbjct: 263 VMLLGHSDSYTRDKVMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSA 322

Query: 356 NPTINSQGNRYAAPTNRFAKEVTKRVETPESEWKGWNWRSEGDMLLNGAYFTPSGAGASA 415
            PTINSQGNR+ AP N FAKEVTKR  T ES+WK WNWRSEGD+ LNGA+FT SGAGA A
Sbjct: 323 GPTINSQGNRFLAPVNPFAKEVTKREYTGESKWKHWNWRSEGDLFLNGAFFTRSGAGAGA 382

Query: 416 SYARASSLGAKSASMVGSITSSAGSLPCRRGHPC 450
           +YARASSL AKS+S+VG++TS +G+L CR G  C
Sbjct: 383 NYARASSLSAKSSSLVGTMTSYSGALNCRAGRRC 416

[Close]

BLAST of Cucsa.194310.1 vs. TAIR10
Match: AT3G24670.1 (AT3G24670.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 686.0 bits (1769), Expect = 1.5e-197
Identity = 330/435 (75.86%), Postives = 367/435 (84.37%), Query Frame = 1


Query: 15  LALLFFLTIALVRKEETSKIRTVSAAEFQSSSNSSMATRVENYDVEQELNNEHAVDNPDE 74
           LAL   L I  +    ++  +        SSSNSS A ++ N D      NEHAV NP+E
Sbjct: 9   LALSTTLIILALCINSSTMAQETEDLNSHSSSNSSTANKLPNDDGAW---NEHAVKNPEE 68

Query: 75  IAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRNRKRLAECGIGFGRNAIGG 134
           +AA V+M I+NSTERR+LGFFSC TGNPIDDCWRCD NWH  RKRLA C IGFGRNAIGG
Sbjct: 69  VAAMVDMKIKNSTERRRLGFFSCATGNPIDDCWRCDRNWHLRRKRLANCAIGFGRNAIGG 128

Query: 135 RDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIRLKQELIMNSFKTIDA 194
           RDGR+YVVTD SD+D VNP+PGTLRHAVIQ++PLWIVFKRDMVI L QELIMNSFKTID 
Sbjct: 129 RDGRYYVVTDPSDHDAVNPRPGTLRHAVIQDRPLWIVFKRDMVITLTQELIMNSFKTIDG 188

Query: 195 RGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPSHFGWRTMADGDAISIF 254
           RGVNV IA GACITIQ+VTNIIIHG+++HDC+ TGNAMVRSSPSH+GWRTMADGDAISIF
Sbjct: 189 RGVNVAIAGGACITIQYVTNIIIHGINVHDCRRTGNAMVRSSPSHYGWRTMADGDAISIF 248

Query: 255 GSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLGHSDSYTKDKQMQVT 314
           GSSHIWIDHNSLSNCADGL+DA+MGSTAITISNN+ THHNEVML+GHSDSYT+DK MQVT
Sbjct: 249 GSSHIWIDHNSLSNCADGLIDAIMGSTAITISNNYMTHHNEVMLMGHSDSYTRDKLMQVT 308

Query: 315 IAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGNRYAAPTNRFA 374
           IAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHW MYAIGGSANPTINSQGNR+ AP N FA
Sbjct: 309 IAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWVMYAIGGSANPTINSQGNRFLAPGNPFA 368

Query: 375 KEVTKRVETPESEWKGWNWRSEGDMLLNGAYFTPSGAGASASYARASSLGAKSASMVGSI 434
           KEVTKRV + + EWK WNWRS+GD++LNGAYFT SGA A ASYARASSLGAK AS+V  +
Sbjct: 369 KEVTKRVGSWQGEWKQWNWRSQGDLMLNGAYFTKSGAAAPASYARASSLGAKPASVVSML 428

Query: 435 TSSAGSLPCRRGHPC 450
           T S+G+L CR G  C
Sbjct: 429 TYSSGALKCRIGMRC 440

[Close]

BLAST of Cucsa.194310.1 vs. TAIR10
Match: AT5G48900.1 (AT5G48900.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 683.3 bits (1762), Expect = 9.9e-197
Identity = 317/385 (82.34%), Postives = 349/385 (90.65%), Query Frame = 1


Query: 65  NEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRNRKRLAECG 124
           +EHAV+NP+E+AA V+MSIRNST RR+LG+FSC TGNPIDDCWRCD  W   RK LA C 
Sbjct: 33  HEHAVENPEEVAAMVDMSIRNSTARRRLGYFSCSTGNPIDDCWRCDRRWQSRRKHLANCA 92

Query: 125 IGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIRLKQEL 184
           IGFGRNAIGGRDGR+YVV+D +D++PVNPKPGTLRHAVIQE+PLWIVFKRDMVI LK+EL
Sbjct: 93  IGFGRNAIGGRDGRYYVVSDPNDDNPVNPKPGTLRHAVIQEEPLWIVFKRDMVITLKEEL 152

Query: 185 IMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPSHFGWRT 244
           IMNSFKTID RGVNVHIANGACITIQFVTNIIIHG+HIHDC+PTGNAMVRSSPSH+GWRT
Sbjct: 153 IMNSFKTIDGRGVNVHIANGACITIQFVTNIIIHGIHIHDCRPTGNAMVRSSPSHYGWRT 212

Query: 245 MADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLGHSDS 304
           MADGD ISIFGSSHIWIDHNSLSNCADGL+DAVM STAITISNN+FTHHNEVMLLGHSD+
Sbjct: 213 MADGDGISIFGSSHIWIDHNSLSNCADGLIDAVMASTAITISNNYFTHHNEVMLLGHSDT 272

Query: 305 YTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGN 364
           YT+DK MQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSA+PTINSQGN
Sbjct: 273 YTRDKVMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSASPTINSQGN 332

Query: 365 RYAAPTNRFAKEVTKRVETPESEWKGWNWRSEGDMLLNGAYFTPSGAGASASYARASSLG 424
           RY AP NRFAKEVTKR    + +W+ WNWRSEGD+ LNGA+FT SG+G  ASYARASSL 
Sbjct: 333 RYLAPRNRFAKEVTKRDYAGQWQWRHWNWRSEGDLFLNGAFFTRSGSGLGASYARASSLA 392

Query: 425 AKSASMVGSITSSAGSLPCRRGHPC 450
           AKS+S+VG IT +AG+L CR G  C
Sbjct: 393 AKSSSLVGVITYNAGALNCRGGRRC 417

[Close]

BLAST of Cucsa.194310.1 vs. NCBI nr
Match: gi|449442407|ref|XP_004138973.1| (PREDICTED: probable pectate lyase 8 isoform X1 [Cucumis sativus])

HSP 1 Score: 927.2 bits (2395), Expect = 1.1e-266
Identity = 449/449 (100.00%), Postives = 449/449 (100.00%), Query Frame = 1


Query: 1   MAALTKWSSLSLFALALLFFLTIALVRKEETSKIRTVSAAEFQSSSNSSMATRVENYDVE 60
           MAALTKWSSLSLFALALLFFLTIALVRKEETSKIRTVSAAEFQSSSNSSMATRVENYDVE
Sbjct: 1   MAALTKWSSLSLFALALLFFLTIALVRKEETSKIRTVSAAEFQSSSNSSMATRVENYDVE 60

Query: 61  QELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRNRKRL 120
           QELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRNRKRL
Sbjct: 61  QELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRNRKRL 120

Query: 121 AECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIRL 180
           AECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIRL
Sbjct: 121 AECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIRL 180

Query: 181 KQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPSHF 240
           KQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPSHF
Sbjct: 181 KQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPSHF 240

Query: 241 GWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLG 300
           GWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLG
Sbjct: 241 GWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLG 300

Query: 301 HSDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTIN 360
           HSDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTIN
Sbjct: 301 HSDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTIN 360

Query: 361 SQGNRYAAPTNRFAKEVTKRVETPESEWKGWNWRSEGDMLLNGAYFTPSGAGASASYARA 420
           SQGNRYAAPTNRFAKEVTKRVETPESEWKGWNWRSEGDMLLNGAYFTPSGAGASASYARA
Sbjct: 361 SQGNRYAAPTNRFAKEVTKRVETPESEWKGWNWRSEGDMLLNGAYFTPSGAGASASYARA 420

Query: 421 SSLGAKSASMVGSITSSAGSLPCRRGHPC 450
           SSLGAKSASMVGSITSSAGSLPCRRGHPC
Sbjct: 421 SSLGAKSASMVGSITSSAGSLPCRRGHPC 449

[Close]

BLAST of Cucsa.194310.1 vs. NCBI nr
Match: gi|659121573|ref|XP_008460726.1| (PREDICTED: probable pectate lyase 8 isoform X1 [Cucumis melo])

HSP 1 Score: 921.0 bits (2379), Expect = 8.0e-265
Identity = 446/449 (99.33%), Postives = 448/449 (99.78%), Query Frame = 1


Query: 1   MAALTKWSSLSLFALALLFFLTIALVRKEETSKIRTVSAAEFQSSSNSSMATRVENYDVE 60
           MAAL+KWSSLSLFALALLFFLTIALVRKEETSKIRTVSAAEFQSSS+SSMATRVENYDVE
Sbjct: 1   MAALSKWSSLSLFALALLFFLTIALVRKEETSKIRTVSAAEFQSSSDSSMATRVENYDVE 60

Query: 61  QELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRNRKRL 120
           QELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRNRKRL
Sbjct: 61  QELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRNRKRL 120

Query: 121 AECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIRL 180
           AECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIRL
Sbjct: 121 AECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIRL 180

Query: 181 KQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPSHF 240
           KQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPSHF
Sbjct: 181 KQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPSHF 240

Query: 241 GWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLG 300
           GWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLG
Sbjct: 241 GWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLG 300

Query: 301 HSDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTIN 360
           HSDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTIN
Sbjct: 301 HSDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTIN 360

Query: 361 SQGNRYAAPTNRFAKEVTKRVETPESEWKGWNWRSEGDMLLNGAYFTPSGAGASASYARA 420
           SQGNRYAAPTN FAKEVTKRVETPESEWKGWNWRSEGDMLLNGAYFTPSGAGASASYARA
Sbjct: 361 SQGNRYAAPTNPFAKEVTKRVETPESEWKGWNWRSEGDMLLNGAYFTPSGAGASASYARA 420

Query: 421 SSLGAKSASMVGSITSSAGSLPCRRGHPC 450
           SSLGAKSASMVGSITSSAGSLPCRRGHPC
Sbjct: 421 SSLGAKSASMVGSITSSAGSLPCRRGHPC 449

[Close]

BLAST of Cucsa.194310.1 vs. NCBI nr
Match: gi|778668540|ref|XP_011649115.1| (PREDICTED: probable pectate lyase 1 isoform X2 [Cucumis sativus])

HSP 1 Score: 884.4 bits (2284), Expect = 8.3e-254
Identity = 431/449 (95.99%), Postives = 431/449 (95.99%), Query Frame = 1


Query: 1   MAALTKWSSLSLFALALLFFLTIALVRKEETSKIRTVSAAEFQSSSNSSMATRVENYDVE 60
           MAALTKWSSLSLFALALLFFLTIALVRKEETSKIR                  VENYDVE
Sbjct: 1   MAALTKWSSLSLFALALLFFLTIALVRKEETSKIR------------------VENYDVE 60

Query: 61  QELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRNRKRL 120
           QELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRNRKRL
Sbjct: 61  QELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRNRKRL 120

Query: 121 AECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIRL 180
           AECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIRL
Sbjct: 121 AECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIRL 180

Query: 181 KQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPSHF 240
           KQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPSHF
Sbjct: 181 KQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPSHF 240

Query: 241 GWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLG 300
           GWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLG
Sbjct: 241 GWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLG 300

Query: 301 HSDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTIN 360
           HSDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTIN
Sbjct: 301 HSDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTIN 360

Query: 361 SQGNRYAAPTNRFAKEVTKRVETPESEWKGWNWRSEGDMLLNGAYFTPSGAGASASYARA 420
           SQGNRYAAPTNRFAKEVTKRVETPESEWKGWNWRSEGDMLLNGAYFTPSGAGASASYARA
Sbjct: 361 SQGNRYAAPTNRFAKEVTKRVETPESEWKGWNWRSEGDMLLNGAYFTPSGAGASASYARA 420

Query: 421 SSLGAKSASMVGSITSSAGSLPCRRGHPC 450
           SSLGAKSASMVGSITSSAGSLPCRRGHPC
Sbjct: 421 SSLGAKSASMVGSITSSAGSLPCRRGHPC 431

[Close]

BLAST of Cucsa.194310.1 vs. NCBI nr
Match: gi|659121575|ref|XP_008460727.1| (PREDICTED: probable pectate lyase 1 isoform X2 [Cucumis melo])

HSP 1 Score: 880.2 bits (2273), Expect = 1.6e-252
Identity = 429/449 (95.55%), Postives = 430/449 (95.77%), Query Frame = 1


Query: 1   MAALTKWSSLSLFALALLFFLTIALVRKEETSKIRTVSAAEFQSSSNSSMATRVENYDVE 60
           MAAL+KWSSLSLFALALLFFLTIALVRKEETSKIR                  VENYDVE
Sbjct: 1   MAALSKWSSLSLFALALLFFLTIALVRKEETSKIR------------------VENYDVE 60

Query: 61  QELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRNRKRL 120
           QELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRNRKRL
Sbjct: 61  QELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRNRKRL 120

Query: 121 AECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIRL 180
           AECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIRL
Sbjct: 121 AECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIRL 180

Query: 181 KQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPSHF 240
           KQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPSHF
Sbjct: 181 KQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPSHF 240

Query: 241 GWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLG 300
           GWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLG
Sbjct: 241 GWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLG 300

Query: 301 HSDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTIN 360
           HSDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTIN
Sbjct: 301 HSDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTIN 360

Query: 361 SQGNRYAAPTNRFAKEVTKRVETPESEWKGWNWRSEGDMLLNGAYFTPSGAGASASYARA 420
           SQGNRYAAPTN FAKEVTKRVETPESEWKGWNWRSEGDMLLNGAYFTPSGAGASASYARA
Sbjct: 361 SQGNRYAAPTNPFAKEVTKRVETPESEWKGWNWRSEGDMLLNGAYFTPSGAGASASYARA 420

Query: 421 SSLGAKSASMVGSITSSAGSLPCRRGHPC 450
           SSLGAKSASMVGSITSSAGSLPCRRGHPC
Sbjct: 421 SSLGAKSASMVGSITSSAGSLPCRRGHPC 431

[Close]

BLAST of Cucsa.194310.1 vs. NCBI nr
Match: gi|1021027419|gb|KZM85206.1| (hypothetical protein DCAR_027372 [Daucus carota subsp. sativus])

HSP 1 Score: 783.5 bits (2022), Expect = 2.0e-223
Identity = 374/442 (84.62%), Postives = 401/442 (90.72%), Query Frame = 1


Query: 9   SLSLFALALLFF-LTIALVRKEETSKIRTVSAAEFQSSSNSSMATRVENYDVEQELNNEH 68
           SLS   L LLF   T  L   +  S I++   AE Q+S NSSMA R+E  DV+ E  NEH
Sbjct: 9   SLSALLLLLLFVGATAVLNNSDHLSSIKSDEKAELQNSVNSSMAARLE--DVDGEGLNEH 68

Query: 69  AVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRNRKRLAECGIGF 128
           AV NPDE+A+ V+MSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRNRKRLA+CGIGF
Sbjct: 69  AVSNPDEVASMVDMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRNRKRLADCGIGF 128

Query: 129 GRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIRLKQELIMN 188
           GRNAIGGRDGRFYVVTDS D+DPVNP+PGTLRHAVIQ++PLWIVFKRDMVI+LKQELIMN
Sbjct: 129 GRNAIGGRDGRFYVVTDSRDDDPVNPRPGTLRHAVIQDRPLWIVFKRDMVIQLKQELIMN 188

Query: 189 SFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPSHFGWRTMAD 248
           SFKTID RGVNVHIANG CITIQFVTN+IIHGLHIHDCKPTGNAMVRSSP+HFGWRTMAD
Sbjct: 189 SFKTIDGRGVNVHIANGGCITIQFVTNVIIHGLHIHDCKPTGNAMVRSSPTHFGWRTMAD 248

Query: 249 GDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLGHSDSYTK 308
           GDAISIFGSSH+W+DHNSLSNCADGLVDAVMGSTAIT+SNNHFTHHNEVMLLGHSDSYT+
Sbjct: 249 GDAISIFGSSHVWVDHNSLSNCADGLVDAVMGSTAITVSNNHFTHHNEVMLLGHSDSYTR 308

Query: 309 DKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGNRYA 368
           DKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSA+PTINSQGNRY 
Sbjct: 309 DKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSASPTINSQGNRYL 368

Query: 369 APTNRFAKEVTKRVETPESEWKGWNWRSEGDMLLNGAYFTPSGAGASASYARASSLGAKS 428
           AP N FAKEVTKRVETP   WKGWNWRSEGD+LLNGAYFTPSGAGA++SYARASSLGAKS
Sbjct: 369 APANPFAKEVTKRVETPVGHWKGWNWRSEGDLLLNGAYFTPSGAGATSSYARASSLGAKS 428

Query: 429 ASMVGSITSSAGSLPCRRGHPC 450
           +SMVG+ITS AG L CRRGH C
Sbjct: 429 SSMVGTITSGAGVLGCRRGHQC 448

The following BLAST results are available for this feature:

BLAST of Cucsa.194310.1 vs. Swiss-Prot


Match Name	E-value	Identity	Description
PLY15_ARATH	3.1e-208	77.78	Probable pectate lyase 15 OS=Arabidopsis thaliana GN=At4g13710 PE=2 SV=1	[more]
PLY1_ARATH	5.5e-205	76.60	Probable pectate lyase 1 OS=Arabidopsis thaliana GN=At1g04680 PE=2 SV=2	[more]
PLY8_ARATH	1.4e-197	81.73	Probable pectate lyase 8 OS=Arabidopsis thaliana GN=At3g07010 PE=2 SV=1	[more]
PLY10_ARATH	2.7e-196	75.86	Probable pectate lyase 10 OS=Arabidopsis thaliana GN=At3g24670 PE=2 SV=2	[more]
PLY20_ARATH	1.8e-195	82.34	Probable pectate lyase 20 OS=Arabidopsis thaliana GN=At5g48900 PE=2 SV=1	[more]

BLAST of Cucsa.194310.1 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LI24_CUCSA	7.8e-267	100.00	Pectate lyase OS=Cucumis sativus GN=Csa_2G155600 PE=3 SV=1	[more]
D4NXR8_PRUPE	1.7e-221	83.33	Pectate lyase OS=Prunus persica GN=PRUPE_ppa005761mg PE=2 SV=1	[more]
A1IIA8_PRUPE	1.1e-220	87.14	Pectate lyase (Fragment) OS=Prunus persica GN=PpPL2 PE=2 SV=1	[more]
A0A0A0L814_CUCSA	4.6e-219	82.18	Pectate lyase OS=Cucumis sativus GN=Csa_3G133180 PE=3 SV=1	[more]
A0A0D2S7U3_GOSRA	3.0e-218	81.90	Pectate lyase OS=Gossypium raimondii GN=B456_006G219800 PE=3 SV=1	[more]

BLAST of Cucsa.194310.1 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G13710.1	1.7e-209	77.78	Pectin lyase-like superfamily protein	[more]
AT1G04680.1	3.1e-206	76.60	Pectin lyase-like superfamily protein	[more]
AT3G07010.1	8.1e-199	81.73	Pectin lyase-like superfamily protein	[more]
AT3G24670.1	1.5e-197	75.86	Pectin lyase-like superfamily protein	[more]
AT5G48900.1	9.9e-197	82.34	Pectin lyase-like superfamily protein	[more]

BLAST of Cucsa.194310.1 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449442407\|ref\|XP_004138973.1\|	1.1e-266	100.00	PREDICTED: probable pectate lyase 8 isoform X1 [Cucumis sativus]	[more]
gi\|659121573\|ref\|XP_008460726.1\|	8.0e-265	99.33	PREDICTED: probable pectate lyase 8 isoform X1 [Cucumis melo]	[more]
gi\|778668540\|ref\|XP_011649115.1\|	8.3e-254	95.99	PREDICTED: probable pectate lyase 1 isoform X2 [Cucumis sativus]	[more]
gi\|659121575\|ref\|XP_008460727.1\|	1.6e-252	95.55	PREDICTED: probable pectate lyase 1 isoform X2 [Cucumis melo]	[more]
gi\|1021027419\|gb\|KZM85206.1\|	2.0e-223	84.62	hypothetical protein DCAR_027372 [Daucus carota subsp. sativus]	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **INTERPRO**
Term	Definition
IPR002022	Pec_lyase
IPR011050	Pectin_lyase_fold/virulence
IPR012334	Pectin_lyas_fold
IPR018082	AmbAllergen

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0045490	pectin catabolic process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0030570	pectate lyase activity

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsa.194310	Cucsa.194310	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsa.194310.1	Cucsa.194310.1-protein	polypeptide

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsa.194310.1.CDS.7	Cucsa.194310.1.CDS.7	CDS
Cucsa.194310.1.CDS.6	Cucsa.194310.1.CDS.6	CDS
Cucsa.194310.1.CDS.5	Cucsa.194310.1.CDS.5	CDS
Cucsa.194310.1.CDS.4	Cucsa.194310.1.CDS.4	CDS
Cucsa.194310.1.CDS.3	Cucsa.194310.1.CDS.3	CDS
Cucsa.194310.1.CDS.2	Cucsa.194310.1.CDS.2	CDS
Cucsa.194310.1.CDS.1	Cucsa.194310.1.CDS.1	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsa.194310.1.three_prime_UTR.1	Cucsa.194310.1.three_prime_UTR.1	three_prime_UTR

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsa.194310.1.five_prime_UTR.1	Cucsa.194310.1.five_prime_UTR.1	five_prime_UTR

InterPro

Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002022	Pectate lyase/Amb allergen	PFAM	PF00544	Pec_lyase_C	coord: 187..364 score: 2.5
IPR002022	Pectate lyase/Amb allergen	SMART	SM00656	amb_all	coord: 175..372 score: 1.3E
IPR011050	Pectin lyase fold/virulence factor	unknown	SSF51126	Pectin lyase-like	coord: 77..443 score: 9.26E
IPR012334	Pectin lyase fold	GENE3D	G3DSA:2.160.20.10		coord: 101..440 score: 1.8E
IPR018082	AmbAllergen	PRINTS	PR00807	AMBALLERGEN	coord: 183..199 score: 5.2E-81coord: 391..415 score: 5.2E-81coord: 349..368 score: 5.2E-81coord: 420..443 score: 5.2E-81coord: 247..268 score: 5.2E-81coord: 123..140 score: 5.2E-81coord: 147..172 score: 5.2E-81coord: 327..346 score: 5.2
None	No IPR available	PANTHER	PTHR31683	FAMILY NOT NAMED	coord: 29..449 score:
None	No IPR available	PANTHER	PTHR31683:SF20	PECTATE LYASE 1-RELATED	coord: 29..449 score: