Cucsa.194310 (gene) Cucumber (Gy14) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cucsa.194310
Type	gene
Organism	Cucumis sativus (Cucumber (Gy14) v1)
Description	Pectate lyase
Location	scaffold01337 : 694824 .. 698779 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CTTTCACTGACATCACCACTTCTCGTTTTCCTCTCTTCTTCTTCTTCTTCTTCCCTTTCCTTTTTTCTCTCTATATTTCTTCTGCTTCCTATAATCTCTCTCTCTCTCTACATTTCGTGCTGTTTTTTCTTTTGACGAGAGAGAGAGAGATAGCAGTTATGGCGGCACTTACTAAATGGTCTTCTCTGTCTCTCTTTGCCCTTGCATTGCTGTTTTTCCTAACAATCGCTCTCGTTCGCAAAGAAGAGACCTCCAAAATTAGGTTCGTTTTCTTTTTCTCTCTTAGAAATCAATTAGATGTGTTTTATATGGTAATTAATTCTAGCCACACTATGTTAATTTCTGCTTACGAGTAGTAAATGCAAGCTTCGCATAGTACCGAACTTCTTTTCGATTGGGAGAATCTTGAATGTTCAATGTGGAATTGTGAGTGTATGATTTACTGTTTTTTCATATGATTTTGGATCAATGTAGCAATATAGAAACGTTATAGAGAAGAAGTAAAGCCTTTAATGGAGTCCTTAAGTTCAACAATTTTGGAAGAAAAGAAACGAGGAATAACGCGAGAATCTCTAAATTCGAAACGCGAACTGCGGTGATTATGTTTTTTATTCCTCTCTTATTATTTTTTAAAAATCAGGACTGTGTCAGCGGCGGAGTTTCAGAGCTCCAGCAACTCATCAATGGCGACTAGGTCAGTGTTCGTTTCACTAAACTCAACTAAATAAAAAGAGGTGAAATTCTGTACCGAACAAAACGATCTCGGCCGTGCGTTTTACCCATCATGCTAATCAGAACCGTTGGATTCTCCTCGGATCCGTGTGGTGTGATGATCCAACTGTAGGTAACGATCTCAACCGTTGAGATCGGGATCCTTGATCTGGACCGTTTGTTCCGTTAACAGTTACTCCCTCTGCTCCAGTGAAATAAATACCACAGTTTATTTCCGTAATTACCCCAAGAGGGTCTTAATTTCCCCAGATTGTTCGATAAAATTCCGGGGTATTTCAGTCATCTTGCCCCTAGGAATAAAAGGACAATTTTCTTTATCTTTTTTCTTTTTAATTCTTTAGGCGGTCGTTTCTCATTTTCCCCATCTCGTGCCGACCAATCGTTCCGCCTCTAATTTCTCTCTCTAAACAAAAGCACTTTTTCAAACGGTTTTCTTCTTCCTGACAGAACGACAGTACGGTAAGGGGCGTAAGAGATCTCTTCCGCACCCACCTTGAGCGTCGAGCCATTAACTTCTTCATTTCATTTTCATTCCTCACGCGCCCACTGATATTCACGCGCTTCTACACTCACATATCTGTTCAGTGTGTTTTAACAGATGGCCTTTGTCACTTTATTGGGTGTTTCTGTTTTCAGGGTGGAGAATTATGATGTGGAGCAAGAACTCAACAATGAACATGCTGTGGATAATCCTGATGAGATTGCTGCTTCTGTTGAGATGTGGGTCTTTTTCTTTTATTGCTTCCATACTCGAATTTATGATTTTTTTTACAAATCTGGGTTATCATTACATGCTCAAATCTTGTTTAAGGAAGTCATCATTAGACTATAGAGAAATTACTTTTATTGAAATTTGGGTCGAATTGGATTTTTTGCTCGTTGTAATGAAATAGAACACATTGCGGATGGTGGGCTTTGTTTTTTGTCTGTATAACAAATTCATAGGATGGGCATTTCTAGACGTTATAATGAAAGAGACCTCATGGTCAAAATGCCAGCATGGTTTTGTCTTGTTCTGTTCGTGTAGTATGGCATTTTCTTGTAGCCGTTGGGATGAAAAATCAGCCCTACCGGTCTCCCCTGTCATTAGGTTCCCGACAAATCCTGTTTTTTCTTTCTATTTTTTCCTCGAGATTTGTACTTGTATCAAGTTTCACTACAATTTCAATGAGAGTTCATATTAGGATCCCTTTTTTATATGGCAGGAGCATCCGTAATAGCACTGAAAGGAGGAAGTTGGGATTCTTCTCCTGTGGAACAGGGAATCCCATTGACGACTGCTGGCGTTGTGACTCCAATTGGCACCGTAACCGTAAACGCCTTGCGGAATGTGGCATTGGATTTGGGCGGAATGCTATTGGTGGACGTGATGGCCGTTTCTATGTTGTCACTGATTCGAGTGACAACGATCCGGTGAACCCGAAGCCAGGGACTCTTCGTCATGCTGTTATTCAAGAGAAACCTCTTTGGATCGTGTTCAAAAGGGATATGGTGATTCGATTGAAACAGGAACTCATCATGAACAGTTTTAAGACAATTGATGCTCGAGGGGTAAATGTACACATTGCTAATGGCGCTTGCATCACAATCCAGTTTGTGACTAACATCATTATCCATGGTCTACACATCCATGATTGCAAACCCACAGGGAATGCCATGGTGAGAAGCTCTCCATCTCATTTTGGGTGGAGAACCATGGCTGATGGAGATGCCATTTCCATCTTTGGTTCAAGCCATATATGGATTGACCACAATTCACTCTCTAATTGTGCTGACGGACTTGTTGATGCTGTTATGGGCTCAACTGCAATCACGATCTCCAACAATCACTTCACTCATCATAATGAGGTACACGTTTTATGGCATTTCTTATATGGAATTAAATTGAAAACACAAATTTATCTGATGGAATTGCATCTTTAACAGGTGATGCTTCTAGGCCACAGTGATTCCTACACGAAGGACAAGCAAATGCAAGTCACCATTGCTTATAACCATTTTGGAGAAGGACTCATTCAAAGAATGCCAAGGTAAATATAAATAGGTATCAATGGTTTGAATTGTGTTTTGAGTTTATTAACTGAGATTTTACCTCACATATTTTCAATAATGTATAGATGTAGGCATGGCTATTTCCATGTGGTGAACAATGACTACACTCACTGGGAGATGTATGCCATTGGTGGGAGTGCAAATCCCACCATCAATAGTCAAGGAAACAGATATGCTGCTCCAACCAACCGTTTTGCCAAAGAGGTACACTCTTCCCAAACTCATCTCAAGCGTAGATTTAGATTCTGATATTCGATATAGCAGTTGACAAATCATGTCGATTCAATACAATACAGGTGACAAAGCGAGTTGAAACACCAGAGAGCGAATGGAAGGGGTGGAATTGGAGATCAGAAGGAGATATGTTGCTGAATGGAGCCTATTTCACTCCATCAGGTGCTGGAGCTTCAGCAAGCTATGCCAGAGCTTCCAGCTTAGGAGCGAAATCTGCTTCCATGGTTGGCTCCATCACTTCTTCTGCAGGTTCACTCCCTTGTCGCCGAGGCCATCCATGCTAGGACTTGTGTCCTCCATCATTTTAACTTCAAACACCAAATGTTAAAAAAGCCATAGACAAAAAAAGCCTTTCCTCATCTTTCGGTTTGATTTTCAAAAAACTTCCATCATTTCTCATCCATCATCTCCTTTGTCCCTTCATTCAAATAGCCATTTTAACAAGTACACATATTTTTTCTTTTTTCAAACTTGTTTTTAAAACCTTCATTCCACCATTGTTGTTCATCTGTTGGTTGCTCTCAACCTCGGCCTGCTTCAGCTGAAAAGAAAATGCCATTTTAGGGGCAGGTGCAGAAGCGTTGTCTCTTTTTATTTTTGGGTCTGTTTATTTTACCATCAAGTTTAACTCATCTTTCCATCTCTTACCACAGTCATTTTGTGATGCAATATTGTTTGGTCACTACACTATGTTTTTACTCTGGGTTTTGTATTTTCTTTTTTCTTTTTCCCCTTCCGAGTCCATGAGAGAGACAACTTAAAGAAGATTACAAAAGTTAAAAAGGAAAGTAGTGATGTTGTTTGTTATTGTTTTTGTCAGTTTTATTGGCCTTTTGATTTTTTTTATTTTTTTTTCACTTAGTGGGTAATGTTTAAGCTTTGGTTACAGTTGGTTATGTTTATATACTCATTATTATAGCCTCCCAAATTGTTTGTGA

mRNA sequence

CTTTCACTGACATCACCACTTCTCGTTTTCCTCtcttcttcttcttcttcttcCCTTTCCTTTTTTCTCTCTATATTTCTTCTGCTTCCTATAATCTCTCTCTCTCTCTACATTTCGTGCTGTTTTTTCTTTTGACGAGAGAGAGAGAGATAGCAGTTATGGCGGCACTTACTAAATGGTCTTCTCTGTCTCTCTTTGCCCTTGCATTGCTGTTTTTCCTAACAATCGCTCTCGTTCGCAAAGAAGAGACCTCCAAAATTAGGACTGTGTCAGCGGCGGAGTTTCAGAGCTCCAGCAACTCATCAATGGCGACTAGGGTGGAGAATTATGATGTGGAGCAAGAACTCAACAATGAACATGCTGTGGATAATCCTGATGAGATTGCTGCTTCTGTTGAGATGAGCATCCGTAATAGCACTGAAAGGAGGAAGTTGGGATTCTTCTCCTGTGGAACAGGGAATCCCATTGACGACTGCTGGCGTTGTGACTCCAATTGGCACCGTAACCGTAAACGCCTTGCGGAATGTGGCATTGGATTTGGGCGGAATGCTATTGGTGGACGTGATGGCCGTTTCTATGTTGTCACTGATTCGAGTGACAACGATCCGGTGAACCCGAAGCCAGGGACTCTTCGTCATGCTGTTATTCAAGAGAAACCTCTTTGGATCGTGTTCAAAAGGGATATGGTGATTCGATTGAAACAGGAACTCATCATGAACAGTTTTAAGACAATTGATGCTCGAGGGGTAAATGTACACATTGCTAATGGCGCTTGCATCACAATCCAGTTTGTGACTAACATCATTATCCATGGTCTACACATCCATGATTGCAAACCCACAGGGAATGCCATGGTGAGAAGCTCTCCATCTCATTTTGGGTGGAGAACCATGGCTGATGGAGATGCCATTTCCATCTTTGGTTCAAGCCATATATGGATTGACCACAATTCACTCTCTAATTGTGCTGACGGACTTGTTGATGCTGTTATGGGCTCAACTGCAATCACGATCTCCAACAATCACTTCACTCATCATAATGAGGTGATGCTTCTAGGCCACAGTGATTCCTACACGAAGGACAAGCAAATGCAAGTCACCATTGCTTATAACCATTTTGGAGAAGGACTCATTCAAAGAATGCCAAGATGTAGGCATGGCTATTTCCATGTGGTGAACAATGACTACACTCACTGGGAGATGTATGCCATTGGTGGGAGTGCAAATCCCACCATCAATAGTCAAGGAAACAGATATGCTGCTCCAACCAACCGTTTTGCCAAAGAGGTGACAAAGCGAGTTGAAACACCAGAGAGCGAATGGAAGGGGTGGAATTGGAGATCAGAAGGAGATATGTTGCTGAATGGAGCCTATTTCACTCCATCAGGTGCTGGAGCTTCAGCAAGCTATGCCAGAGCTTCCAGCTTAGGAGCGAAATCTGCTTCCATGGTTGGCTCCATCACTTCTTCTGCAGGTTCACTCCCTTGTCGCCGAGGCCATCCATGCTAGGACTTGTGTCCTCCATCATTTTAACTTCAAACACCAAATGTTAAAAAAGCCATAGACAAAAAAAGCCTTTCCTCATCTTTCGGTTTGATTTTCAAAAAACTTCCATCATTTCTCATCCATCATCTCCTTTGTCCCTTCATTCAAATAGCCATTTTAACAAGTACACATATTTTTTCTTTTTTCAAACTTGTTTTTAAAACCTTCATTCCACCATTGTTGTTCATCTGTTGGTTGCTCTCAACCTCGGCCTGCTTCAGCTGAAAAGAAAATGCCATTTTAGGGGCAGGTGCAGAAGCGTTGTCTCTTTTTATTTTTGGGTCTGTTTATTTTACCATCAAGTTTAACTCATCTTTCCATCTCTTACCACAGTCATTTTGTGATGCAATATTGTTTGGTCACTACACTATGTTTTTACTCTGGGTTTTGTATTTTCTTTTTTCTTTTTCCCCTTCCGAGTCCATGAGAGAGACAACTTAAAGAAGATTACAAAAGTTAAAAAGGAAAGTAGTGATGTTGTTTGTTATTGTTTTTGTCAGTTTTATTGGCCTTTTGATTTTTTTTATTTTTTTTTCACTTAGTGGGTAATGTTTAAGCTTTGGTTACAGTTGGTTATGTTTATATACTCATTATTATAGCCTCCCAAATTGTTTGTGA

Coding sequence (CDS)

ATGGCGGCACTTACTAAATGGTCTTCTCTGTCTCTCTTTGCCCTTGCATTGCTGTTTTTCCTAACAATCGCTCTCGTTCGCAAAGAAGAGACCTCCAAAATTAGGACTGTGTCAGCGGCGGAGTTTCAGAGCTCCAGCAACTCATCAATGGCGACTAGGGTGGAGAATTATGATGTGGAGCAAGAACTCAACAATGAACATGCTGTGGATAATCCTGATGAGATTGCTGCTTCTGTTGAGATGAGCATCCGTAATAGCACTGAAAGGAGGAAGTTGGGATTCTTCTCCTGTGGAACAGGGAATCCCATTGACGACTGCTGGCGTTGTGACTCCAATTGGCACCGTAACCGTAAACGCCTTGCGGAATGTGGCATTGGATTTGGGCGGAATGCTATTGGTGGACGTGATGGCCGTTTCTATGTTGTCACTGATTCGAGTGACAACGATCCGGTGAACCCGAAGCCAGGGACTCTTCGTCATGCTGTTATTCAAGAGAAACCTCTTTGGATCGTGTTCAAAAGGGATATGGTGATTCGATTGAAACAGGAACTCATCATGAACAGTTTTAAGACAATTGATGCTCGAGGGGTAAATGTACACATTGCTAATGGCGCTTGCATCACAATCCAGTTTGTGACTAACATCATTATCCATGGTCTACACATCCATGATTGCAAACCCACAGGGAATGCCATGGTGAGAAGCTCTCCATCTCATTTTGGGTGGAGAACCATGGCTGATGGAGATGCCATTTCCATCTTTGGTTCAAGCCATATATGGATTGACCACAATTCACTCTCTAATTGTGCTGACGGACTTGTTGATGCTGTTATGGGCTCAACTGCAATCACGATCTCCAACAATCACTTCACTCATCATAATGAGGTGATGCTTCTAGGCCACAGTGATTCCTACACGAAGGACAAGCAAATGCAAGTCACCATTGCTTATAACCATTTTGGAGAAGGACTCATTCAAAGAATGCCAAGATGTAGGCATGGCTATTTCCATGTGGTGAACAATGACTACACTCACTGGGAGATGTATGCCATTGGTGGGAGTGCAAATCCCACCATCAATAGTCAAGGAAACAGATATGCTGCTCCAACCAACCGTTTTGCCAAAGAGGTGACAAAGCGAGTTGAAACACCAGAGAGCGAATGGAAGGGGTGGAATTGGAGATCAGAAGGAGATATGTTGCTGAATGGAGCCTATTTCACTCCATCAGGTGCTGGAGCTTCAGCAAGCTATGCCAGAGCTTCCAGCTTAGGAGCGAAATCTGCTTCCATGGTTGGCTCCATCACTTCTTCTGCAGGTTCACTCCCTTGTCGCCGAGGCCATCCATGCTAG

Protein sequence

MAALTKWSSLSLFALALLFFLTIALVRKEETSKIRTVSAAEFQSSSNSSMATRVENYDVEQELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRNRKRLAECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIRLKQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPSHFGWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLGHSDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGNRYAAPTNRFAKEVTKRVETPESEWKGWNWRSEGDMLLNGAYFTPSGAGASASYARASSLGAKSASMVGSITSSAGSLPCRRGHPC*

Homology

[Close]

BLAST of Cucsa.194310 vs. Swiss-Prot
Match: PLY15_ARATH (Probable pectate lyase 15 OS=Arabidopsis thaliana GN=At4g13710 PE=2 SV=1)

HSP 1 Score: 725.7 bits (1872), Expect = 3.1e-208
Identity = 357/459 (77.78%), Postives = 393/459 (85.62%), Query Frame = 1


Query: 10  LSLFALALLFFLT--IALVRKEET-----------SKIRTVSAAEFQSSSNSSMA-TRVE 69
           L + AL  + F    I+L RK +T           + IR +   EFQS ++S+MA TR++
Sbjct: 16  LVVLALTAMIFRNSEISLSRKLKTEVIQSSNSSTMAAIRKLKTEEFQSLNSSTMAATRLD 75

Query: 70  NYDVEQELNNEHAV-DNPD----EIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCD 129
               +Q+    HAV D+PD    E+A  V+MS +N T RRKLGFFSCGTGNPIDDCWRCD
Sbjct: 76  GEPQQQQ----HAVADDPDMVADEVAKLVQMSEQNRTARRKLGFFSCGTGNPIDDCWRCD 135

Query: 130 SNWHRNRKRLAECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWI 189
            NWH+NRKRLA+CGIGFGRNAIGGRDGRFY+VTD +D D VNPKPGTLRHAVIQE+PLWI
Sbjct: 136 RNWHKNRKRLADCGIGFGRNAIGGRDGRFYIVTDPTDEDVVNPKPGTLRHAVIQEEPLWI 195

Query: 190 VFKRDMVIRLKQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGN 249
           VFKRDMVI LKQELIMNSFKTIDARG NVHIANGACITIQF+TN+IIHGLHIHDCKPTGN
Sbjct: 196 VFKRDMVIELKQELIMNSFKTIDARGSNVHIANGACITIQFITNVIIHGLHIHDCKPTGN 255

Query: 250 AMVRSSPSHFGWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHF 309
           AMVRSSPSHFGWRTMADGDA+SIFGSSHIWIDHNSLS+CADGLVDAVMGSTAIT+SNNHF
Sbjct: 256 AMVRSSPSHFGWRTMADGDAVSIFGSSHIWIDHNSLSHCADGLVDAVMGSTAITVSNNHF 315

Query: 310 THHNEVMLLGHSDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYA 369
           THHNEVMLLGHSDSYTKDK MQVTIAYNHFGEGL+QRMPRCRHGYFHVVNNDYTHWEMYA
Sbjct: 316 THHNEVMLLGHSDSYTKDKLMQVTIAYNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMYA 375

Query: 370 IGGSANPTINSQGNRYAAPTNRFAKEVTKRVETPESEWKGWNWRSEGDMLLNGAYFTPSG 429
           IGGSA PTINSQGNRYAAP +RFAKEVTKRVET  SEWK WNWRSEGD+LLNGA+F PSG
Sbjct: 376 IGGSAEPTINSQGNRYAAPMDRFAKEVTKRVETDASEWKKWNWRSEGDLLLNGAFFRPSG 435

Query: 430 AGASASYARASSLGAKSASMVGSITSSAGSLPCRRGHPC 450
           AGASASY RASSL AK +SMV +ITS+AG+L CR+G PC
Sbjct: 436 AGASASYGRASSLAAKPSSMVDTITSTAGALGCRKGRPC 470

[Close]

BLAST of Cucsa.194310 vs. Swiss-Prot
Match: PLY1_ARATH (Probable pectate lyase 1 OS=Arabidopsis thaliana GN=At1g04680 PE=2 SV=2)

HSP 1 Score: 714.9 bits (1844), Expect = 5.5e-205
Identity = 347/453 (76.60%), Postives = 376/453 (83.00%), Query Frame = 1


Query: 1   MAALTKWSSLSLFALALLFFLTIALVRKEETSKIRTVSAAEFQSSSNSSMATRVENYDVE 60
           MA L  W    L  + LLFF                V A E  +  N S   R +  +  
Sbjct: 1   MAVLPTWL---LAMMCLLFF----------------VGAMENTTHDNISSLPRSDETEW- 60

Query: 61  QELNNEHAVDNPDEIAASV----EMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRN 120
               N+HAV NPDE+A  V    EMS+RN TERRKLG+F+CGTGNPIDDCWRCD NWH+N
Sbjct: 61  ----NQHAVTNPDEVADEVLALTEMSVRNHTERRKLGYFTCGTGNPIDDCWRCDPNWHKN 120

Query: 121 RKRLAECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDM 180
           RKRLA+CGIGFGRNAIGGRDGRFYVVTD  D++PVNP+PGTLRHAVIQ++PLWIVFKRDM
Sbjct: 121 RKRLADCGIGFGRNAIGGRDGRFYVVTDPRDDNPVNPRPGTLRHAVIQDRPLWIVFKRDM 180

Query: 181 VIRLKQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSS 240
           VI+LKQELI+NSFKTID RG NVHIANG CITIQFVTN+I+HGLHIHDCKPTGNAMVRSS
Sbjct: 181 VIQLKQELIVNSFKTIDGRGANVHIANGGCITIQFVTNVIVHGLHIHDCKPTGNAMVRSS 240

Query: 241 PSHFGWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEV 300
            +HFGWRTMADGDAISIFGSSH+WIDHNSLS+CADGLVDAVMGSTAITISNNH THHNEV
Sbjct: 241 ETHFGWRTMADGDAISIFGSSHVWIDHNSLSHCADGLVDAVMGSTAITISNNHLTHHNEV 300

Query: 301 MLLGHSDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSAN 360
           MLLGHSDSY +DK MQVTIAYNHFG GLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSAN
Sbjct: 301 MLLGHSDSYMRDKAMQVTIAYNHFGVGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSAN 360

Query: 361 PTINSQGNRYAAPTNRFAKEVTKRVETPESEWKGWNWRSEGDMLLNGAYFTPSGAGASAS 420
           PTINSQGNRYAAP N FAKEVTKRV+TP S WKGWNWRSEGD+L NGAYFT SGA AS S
Sbjct: 361 PTINSQGNRYAAPKNPFAKEVTKRVDTPASHWKGWNWRSEGDLLQNGAYFTSSGAAASGS 420

Query: 421 YARASSLGAKSASMVGSITSSAGSLPCRRGHPC 450
           YARASSL AKS+S+VG ITS AG+LPCRRG  C
Sbjct: 421 YARASSLSAKSSSLVGHITSDAGALPCRRGRQC 429

[Close]

BLAST of Cucsa.194310 vs. Swiss-Prot
Match: PLY8_ARATH (Probable pectate lyase 8 OS=Arabidopsis thaliana GN=At3g07010 PE=2 SV=1)

HSP 1 Score: 690.3 bits (1780), Expect = 1.4e-197
Identity = 322/394 (81.73%), Postives = 354/394 (89.85%), Query Frame = 1


Query: 56  NYDVEQELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHR 115
           N     E  +EHAV+NPDE+AA V+MSIRNSTERR+LG+FSC TGNPIDDCWRCD  W  
Sbjct: 23  NASRSNETWHEHAVENPDEVAAMVDMSIRNSTERRRLGYFSCATGNPIDDCWRCDRKWQL 82

Query: 116 NRKRLAECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRD 175
            RKRLA+C IGFGRNAIGGRDGRFYVVTD  D+DPVNP PGTLRHAVIQ++PLWI+FKRD
Sbjct: 83  RRKRLADCSIGFGRNAIGGRDGRFYVVTDPGDDDPVNPIPGTLRHAVIQDEPLWIIFKRD 142

Query: 176 MVIRLKQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRS 235
           MVI LKQELIMNSFKTID RGVNVHIANGAC+TIQ+VTNII+HG+H+HDCKPTGNAMVRS
Sbjct: 143 MVITLKQELIMNSFKTIDGRGVNVHIANGACLTIQYVTNIIVHGIHVHDCKPTGNAMVRS 202

Query: 236 SPSHFGWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNE 295
           SPSH+G+R+MADGDAISIFGSSHIWIDHNSLSNCADGLVDAVM STAIT+SNN FTHHNE
Sbjct: 203 SPSHYGFRSMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMSSTAITVSNNFFTHHNE 262

Query: 296 VMLLGHSDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSA 355
           VMLLGHSDSYT+DK MQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSA
Sbjct: 263 VMLLGHSDSYTRDKVMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSA 322

Query: 356 NPTINSQGNRYAAPTNRFAKEVTKRVETPESEWKGWNWRSEGDMLLNGAYFTPSGAGASA 415
            PTINSQGNR+ AP N FAKEVTKR  T ES+WK WNWRSEGD+ LNGA+FT SGAGA A
Sbjct: 323 GPTINSQGNRFLAPVNPFAKEVTKREYTGESKWKHWNWRSEGDLFLNGAFFTRSGAGAGA 382

Query: 416 SYARASSLGAKSASMVGSITSSAGSLPCRRGHPC 450
           +YARASSL AKS+S+VG++TS +G+L CR G  C
Sbjct: 383 NYARASSLSAKSSSLVGTMTSYSGALNCRAGRRC 416

[Close]

BLAST of Cucsa.194310 vs. Swiss-Prot
Match: PLY10_ARATH (Probable pectate lyase 10 OS=Arabidopsis thaliana GN=At3g24670 PE=2 SV=2)

HSP 1 Score: 686.0 bits (1769), Expect = 2.7e-196
Identity = 330/435 (75.86%), Postives = 367/435 (84.37%), Query Frame = 1


Query: 15  LALLFFLTIALVRKEETSKIRTVSAAEFQSSSNSSMATRVENYDVEQELNNEHAVDNPDE 74
           LAL   L I  +    ++  +        SSSNSS A ++ N D      NEHAV NP+E
Sbjct: 9   LALSTTLIILALCINSSTMAQETEDLNSHSSSNSSTANKLPNDDGAW---NEHAVKNPEE 68

Query: 75  IAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRNRKRLAECGIGFGRNAIGG 134
           +AA V+M I+NSTERR+LGFFSC TGNPIDDCWRCD NWH  RKRLA C IGFGRNAIGG
Sbjct: 69  VAAMVDMKIKNSTERRRLGFFSCATGNPIDDCWRCDRNWHLRRKRLANCAIGFGRNAIGG 128

Query: 135 RDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIRLKQELIMNSFKTIDA 194
           RDGR+YVVTD SD+D VNP+PGTLRHAVIQ++PLWIVFKRDMVI L QELIMNSFKTID 
Sbjct: 129 RDGRYYVVTDPSDHDAVNPRPGTLRHAVIQDRPLWIVFKRDMVITLTQELIMNSFKTIDG 188

Query: 195 RGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPSHFGWRTMADGDAISIF 254
           RGVNV IA GACITIQ+VTNIIIHG+++HDC+ TGNAMVRSSPSH+GWRTMADGDAISIF
Sbjct: 189 RGVNVAIAGGACITIQYVTNIIIHGINVHDCRRTGNAMVRSSPSHYGWRTMADGDAISIF 248

Query: 255 GSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLGHSDSYTKDKQMQVT 314
           GSSHIWIDHNSLSNCADGL+DA+MGSTAITISNN+ THHNEVML+GHSDSYT+DK MQVT
Sbjct: 249 GSSHIWIDHNSLSNCADGLIDAIMGSTAITISNNYMTHHNEVMLMGHSDSYTRDKLMQVT 308

Query: 315 IAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGNRYAAPTNRFA 374
           IAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHW MYAIGGSANPTINSQGNR+ AP N FA
Sbjct: 309 IAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWVMYAIGGSANPTINSQGNRFLAPGNPFA 368

Query: 375 KEVTKRVETPESEWKGWNWRSEGDMLLNGAYFTPSGAGASASYARASSLGAKSASMVGSI 434
           KEVTKRV + + EWK WNWRS+GD++LNGAYFT SGA A ASYARASSLGAK AS+V  +
Sbjct: 369 KEVTKRVGSWQGEWKQWNWRSQGDLMLNGAYFTKSGAAAPASYARASSLGAKPASVVSML 428

Query: 435 TSSAGSLPCRRGHPC 450
           T S+G+L CR G  C
Sbjct: 429 TYSSGALKCRIGMRC 440

[Close]

BLAST of Cucsa.194310 vs. Swiss-Prot
Match: PLY20_ARATH (Probable pectate lyase 20 OS=Arabidopsis thaliana GN=At5g48900 PE=2 SV=1)

HSP 1 Score: 683.3 bits (1762), Expect = 1.8e-195
Identity = 317/385 (82.34%), Postives = 349/385 (90.65%), Query Frame = 1


Query: 65  NEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRNRKRLAECG 124
           +EHAV+NP+E+AA V+MSIRNST RR+LG+FSC TGNPIDDCWRCD  W   RK LA C 
Sbjct: 33  HEHAVENPEEVAAMVDMSIRNSTARRRLGYFSCSTGNPIDDCWRCDRRWQSRRKHLANCA 92

Query: 125 IGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIRLKQEL 184
           IGFGRNAIGGRDGR+YVV+D +D++PVNPKPGTLRHAVIQE+PLWIVFKRDMVI LK+EL
Sbjct: 93  IGFGRNAIGGRDGRYYVVSDPNDDNPVNPKPGTLRHAVIQEEPLWIVFKRDMVITLKEEL 152

Query: 185 IMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPSHFGWRT 244
           IMNSFKTID RGVNVHIANGACITIQFVTNIIIHG+HIHDC+PTGNAMVRSSPSH+GWRT
Sbjct: 153 IMNSFKTIDGRGVNVHIANGACITIQFVTNIIIHGIHIHDCRPTGNAMVRSSPSHYGWRT 212

Query: 245 MADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLGHSDS 304
           MADGD ISIFGSSHIWIDHNSLSNCADGL+DAVM STAITISNN+FTHHNEVMLLGHSD+
Sbjct: 213 MADGDGISIFGSSHIWIDHNSLSNCADGLIDAVMASTAITISNNYFTHHNEVMLLGHSDT 272

Query: 305 YTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGN 364
           YT+DK MQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSA+PTINSQGN
Sbjct: 273 YTRDKVMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSASPTINSQGN 332

Query: 365 RYAAPTNRFAKEVTKRVETPESEWKGWNWRSEGDMLLNGAYFTPSGAGASASYARASSLG 424
           RY AP NRFAKEVTKR    + +W+ WNWRSEGD+ LNGA+FT SG+G  ASYARASSL 
Sbjct: 333 RYLAPRNRFAKEVTKRDYAGQWQWRHWNWRSEGDLFLNGAFFTRSGSGLGASYARASSLA 392

Query: 425 AKSASMVGSITSSAGSLPCRRGHPC 450
           AKS+S+VG IT +AG+L CR G  C
Sbjct: 393 AKSSSLVGVITYNAGALNCRGGRRC 417

[Close]

BLAST of Cucsa.194310 vs. TrEMBL
Match: A0A0A0LI24_CUCSA (Pectate lyase OS=Cucumis sativus GN=Csa_2G155600 PE=3 SV=1)

HSP 1 Score: 927.2 bits (2395), Expect = 7.8e-267
Identity = 449/449 (100.00%), Postives = 449/449 (100.00%), Query Frame = 1


Query: 1   MAALTKWSSLSLFALALLFFLTIALVRKEETSKIRTVSAAEFQSSSNSSMATRVENYDVE 60
           MAALTKWSSLSLFALALLFFLTIALVRKEETSKIRTVSAAEFQSSSNSSMATRVENYDVE
Sbjct: 1   MAALTKWSSLSLFALALLFFLTIALVRKEETSKIRTVSAAEFQSSSNSSMATRVENYDVE 60

Query: 61  QELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRNRKRL 120
           QELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRNRKRL
Sbjct: 61  QELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRNRKRL 120

Query: 121 AECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIRL 180
           AECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIRL
Sbjct: 121 AECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIRL 180

Query: 181 KQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPSHF 240
           KQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPSHF
Sbjct: 181 KQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPSHF 240

Query: 241 GWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLG 300
           GWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLG
Sbjct: 241 GWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLG 300

Query: 301 HSDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTIN 360
           HSDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTIN
Sbjct: 301 HSDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTIN 360

Query: 361 SQGNRYAAPTNRFAKEVTKRVETPESEWKGWNWRSEGDMLLNGAYFTPSGAGASASYARA 420
           SQGNRYAAPTNRFAKEVTKRVETPESEWKGWNWRSEGDMLLNGAYFTPSGAGASASYARA
Sbjct: 361 SQGNRYAAPTNRFAKEVTKRVETPESEWKGWNWRSEGDMLLNGAYFTPSGAGASASYARA 420

Query: 421 SSLGAKSASMVGSITSSAGSLPCRRGHPC 450
           SSLGAKSASMVGSITSSAGSLPCRRGHPC
Sbjct: 421 SSLGAKSASMVGSITSSAGSLPCRRGHPC 449

[Close]

BLAST of Cucsa.194310 vs. TrEMBL
Match: D4NXR8_PRUPE (Pectate lyase OS=Prunus persica GN=PRUPE_ppa005761mg PE=2 SV=1)

HSP 1 Score: 776.5 bits (2004), Expect = 1.7e-221
Identity = 370/444 (83.33%), Postives = 399/444 (89.86%), Query Frame = 1


Query: 6   KWSSLSLFALALLFFLTIALVRKEETSKIRTVSAAEFQSSSNSSMATRVENYDVEQELNN 65
           +W + +  A+ L+     A+    E S  R     E QSSSNSSMA RV   D   E  N
Sbjct: 8   RWVTGAFLAVLLVLCFVAAIA---EISGNRNGGTEELQSSSNSSMAARVAEDD---ESFN 67

Query: 66  EHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRNRKRLAECGI 125
           +HAVD+P+E+ A V+MSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNW +NRKRLA+CGI
Sbjct: 68  KHAVDDPEEVVAMVDMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWQKNRKRLADCGI 127

Query: 126 GFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIRLKQELI 185
           GFGRNAIGGRDGRFYVVTD  D+DPVNP+PGTLRHAVIQ +PLWIVFKRDMVI+LKQELI
Sbjct: 128 GFGRNAIGGRDGRFYVVTDPGDDDPVNPRPGTLRHAVIQNEPLWIVFKRDMVIQLKQELI 187

Query: 186 MNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPSHFGWRTM 245
           MNSFKTID RGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNA+VRSSPSHFGWRTM
Sbjct: 188 MNSFKTIDGRGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNALVRSSPSHFGWRTM 247

Query: 246 ADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLGHSDSY 305
           ADGDA+SIFGSSHIW+DHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLGHSDSY
Sbjct: 248 ADGDAVSIFGSSHIWVDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLGHSDSY 307

Query: 306 TKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGNR 365
           T+DK MQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSA PTINSQGNR
Sbjct: 308 TRDKAMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSAEPTINSQGNR 367

Query: 366 YAAPTNRFAKEVTKRVETPESEWKGWNWRSEGDMLLNGAYFTPSGAGASASYARASSLGA 425
           YAAPTN FAKEVTKRVETP ++WK WNWRSEGD+LLNGAYFTPSGAGASASYARASSLGA
Sbjct: 368 YAAPTNPFAKEVTKRVETPTTQWKSWNWRSEGDLLLNGAYFTPSGAGASASYARASSLGA 427

Query: 426 KSASMVGSITSSAGSLPCRRGHPC 450
           KS++MVG+ITS +G+LPCRRGHPC
Sbjct: 428 KSSAMVGAITSGSGALPCRRGHPC 445

[Close]

BLAST of Cucsa.194310 vs. TrEMBL
Match: A1IIA8_PRUPE (Pectate lyase (Fragment) OS=Prunus persica GN=PpPL2 PE=2 SV=1)

HSP 1 Score: 773.9 bits (1997), Expect = 1.1e-220
Identity = 366/420 (87.14%), Postives = 389/420 (92.62%), Query Frame = 1


Query: 30  ETSKIRTVSAAEFQSSSNSSMATRVENYDVEQELNNEHAVDNPDEIAASVEMSIRNSTER 89
           E S  R     E QSSSNSSMA RV   D   E  N+HAVD+P+E+ A V+MSIRNSTER
Sbjct: 9   EISGNRNGGTEELQSSSNSSMAARVAEDD---ESFNKHAVDDPEEVVAMVDMSIRNSTER 68

Query: 90  RKLGFFSCGTGNPIDDCWRCDSNWHRNRKRLAECGIGFGRNAIGGRDGRFYVVTDSSDND 149
           RKLGFFSCGTGNPIDDCWRCDSNW +NRKRLA+CGIGFGRNAIGGRDGRFYVVTD  D+D
Sbjct: 69  RKLGFFSCGTGNPIDDCWRCDSNWQKNRKRLADCGIGFGRNAIGGRDGRFYVVTDPGDDD 128

Query: 150 PVNPKPGTLRHAVIQEKPLWIVFKRDMVIRLKQELIMNSFKTIDARGVNVHIANGACITI 209
           PVNP+PGTLRHAVIQ +PLWIVFKRDMVI+LKQELIMNSFKTID RGVNVHIANGACITI
Sbjct: 129 PVNPRPGTLRHAVIQNEPLWIVFKRDMVIQLKQELIMNSFKTIDGRGVNVHIANGACITI 188

Query: 210 QFVTNIIIHGLHIHDCKPTGNAMVRSSPSHFGWRTMADGDAISIFGSSHIWIDHNSLSNC 269
           QFVTNIIIHGLHIHDCKPTGNA+VRSSPSHFGWRTMADGDA+SIFGSSHIW+DHNSLSNC
Sbjct: 189 QFVTNIIIHGLHIHDCKPTGNALVRSSPSHFGWRTMADGDAVSIFGSSHIWVDHNSLSNC 248

Query: 270 ADGLVDAVMGSTAITISNNHFTHHNEVMLLGHSDSYTKDKQMQVTIAYNHFGEGLIQRMP 329
           ADGLVDAVMGSTAITISNNHFTHHNEVMLLGHSDSYT+DK MQVTIAYNHFGEGLIQRMP
Sbjct: 249 ADGLVDAVMGSTAITISNNHFTHHNEVMLLGHSDSYTRDKAMQVTIAYNHFGEGLIQRMP 308

Query: 330 RCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGNRYAAPTNRFAKEVTKRVETPESEWK 389
           RCRHGYFHVVNNDYTHWEMYAIGGSA PTINSQGNRYAAPTN FAKEVTKRVETP ++WK
Sbjct: 309 RCRHGYFHVVNNDYTHWEMYAIGGSAEPTINSQGNRYAAPTNPFAKEVTKRVETPTTQWK 368

Query: 390 GWNWRSEGDMLLNGAYFTPSGAGASASYARASSLGAKSASMVGSITSSAGSLPCRRGHPC 449
            WNWRSEGD+LLNGAYFTPSGAGASASYARASSLGAKS++MVG+ITS +G+LPCRRGHPC
Sbjct: 369 SWNWRSEGDLLLNGAYFTPSGAGASASYARASSLGAKSSAMVGAITSGSGALPCRRGHPC 425

[Close]

BLAST of Cucsa.194310 vs. TrEMBL
Match: A0A0A0L814_CUCSA (Pectate lyase OS=Cucumis sativus GN=Csa_3G133180 PE=3 SV=1)

HSP 1 Score: 768.5 bits (1983), Expect = 4.6e-219
Identity = 369/449 (82.18%), Postives = 403/449 (89.76%), Query Frame = 1


Query: 1   MAALTKWSSLSLFALALLFFLTIALVRKEETSKIRTVSAAEFQSSSNSSMATRVENYDVE 60
           MA   KW  L L  + +LF   IA   KE   +I      + QSSSNS+MA R+   D  
Sbjct: 3   MAESGKWIRLILLVVFMLFVGAIARAPKE-IPEIENGGTEKLQSSSNSTMAVRL---DEA 62

Query: 61  QELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRNRKRL 120
           ++L NEHAVDNP+EIAA V+MSIRN+T RR+LGFFSCGTGNPIDDCWRCD NW +NRKRL
Sbjct: 63  KDLGNEHAVDNPEEIAAMVDMSIRNATARRELGFFSCGTGNPIDDCWRCDPNWQQNRKRL 122

Query: 121 AECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIRL 180
           A+CGIGFGRNAIGGRDGRFYVVTDS D++PVNPKPGTLRHAVIQ++PLWIVFKRDMVI+L
Sbjct: 123 ADCGIGFGRNAIGGRDGRFYVVTDSGDDNPVNPKPGTLRHAVIQDEPLWIVFKRDMVIQL 182

Query: 181 KQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPSHF 240
           KQELIMNSFKTIDARG NVHIANGAC+TIQFVTN+I+HGL+IHDCKPTGNAMVRSSP+H 
Sbjct: 183 KQELIMNSFKTIDARGTNVHIANGACLTIQFVTNVIVHGLNIHDCKPTGNAMVRSSPNHV 242

Query: 241 GWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLG 300
           GWRT+ADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNN+FTHHNEVMLLG
Sbjct: 243 GWRTIADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNYFTHHNEVMLLG 302

Query: 301 HSDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTIN 360
           HSDSY +DK MQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTIN
Sbjct: 303 HSDSYVRDKLMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTIN 362

Query: 361 SQGNRYAAPTNRFAKEVTKRVETPESEWKGWNWRSEGDMLLNGAYFTPSGAGASASYARA 420
           SQGNRYAAPTN FAKEVTKRVET ESEWKGWNWRSEGD+LLNGA+F PSGAGAS+SYARA
Sbjct: 363 SQGNRYAAPTNPFAKEVTKRVETSESEWKGWNWRSEGDLLLNGAFFIPSGAGASSSYARA 422

Query: 421 SSLGAKSASMVGSITSSAGSLPCRRGHPC 450
           SSLGAKS+SMVG+ITS+AG+L CRRGH C
Sbjct: 423 SSLGAKSSSMVGTITSNAGALSCRRGHAC 447

[Close]

BLAST of Cucsa.194310 vs. TrEMBL
Match: A0A0D2S7U3_GOSRA (Pectate lyase OS=Gossypium raimondii GN=B456_006G219800 PE=3 SV=1)

HSP 1 Score: 765.8 bits (1976), Expect = 3.0e-218
Identity = 371/453 (81.90%), Postives = 398/453 (87.86%), Query Frame = 1


Query: 1   MAALTKWSSLSLFALALLFFLTIALVRKEETSKIRTVSAAEFQSSSNSSMATRVENYDVE 60
           MA    W+  SL  L LL    +A VR    S    V   + QSS NS+MA R E    E
Sbjct: 1   MAVSNNWAFASLALLFLLLVAAMATVRTGNVSS--NVETEKVQSSKNSTMAARSE----E 60

Query: 61  QELNNEHAVDNPDEIAASVE----MSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRN 120
            E  NEHAV +P+ +AA VE    M+IRN TERRKLGFF+CGTGNPIDDCWRCD NW +N
Sbjct: 61  VEPLNEHAVPDPEAVAAEVESIIDMNIRNVTERRKLGFFTCGTGNPIDDCWRCDPNWQKN 120

Query: 121 RKRLAECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDM 180
           RKRLA+CGIGFGRNAIGGRDGRFYVVTD +D+DPVNPKPGTLRHAVIQ++PLWIVFKRDM
Sbjct: 121 RKRLADCGIGFGRNAIGGRDGRFYVVTDPNDDDPVNPKPGTLRHAVIQDEPLWIVFKRDM 180

Query: 181 VIRLKQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSS 240
           VIRLKQELIMNSFKTID RGVNVHIANGACITIQ+VTN+IIHGLHIHDCKPTGNAMVRSS
Sbjct: 181 VIRLKQELIMNSFKTIDGRGVNVHIANGACITIQYVTNVIIHGLHIHDCKPTGNAMVRSS 240

Query: 241 PSHFGWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEV 300
           PSHFGWRTMADGDAISIFGSSHIW+DHNSLSNCADGLVDAVMGSTAITISNNH THHNEV
Sbjct: 241 PSHFGWRTMADGDAISIFGSSHIWVDHNSLSNCADGLVDAVMGSTAITISNNHMTHHNEV 300

Query: 301 MLLGHSDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSAN 360
           MLLGHSDSYT+DKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSAN
Sbjct: 301 MLLGHSDSYTRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSAN 360

Query: 361 PTINSQGNRYAAPTNRFAKEVTKRVETPESEWKGWNWRSEGDMLLNGAYFTPSGAGASAS 420
           PTINSQGNRYAAPTN FAKEVTKRV+T E +WK WNWRSEGD+LLNGAYFTPSGAGASAS
Sbjct: 361 PTINSQGNRYAAPTNPFAKEVTKRVDTAEGQWKSWNWRSEGDLLLNGAYFTPSGAGASAS 420

Query: 421 YARASSLGAKSASMVGSITSSAGSLPCRRGHPC 450
           YARASSLGAKS+SMVG++TS+AG+LPCRRG  C
Sbjct: 421 YARASSLGAKSSSMVGAMTSNAGALPCRRGKQC 447

[Close]

BLAST of Cucsa.194310 vs. TAIR10
Match: AT4G13710.1 (AT4G13710.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 725.7 bits (1872), Expect = 1.7e-209
Identity = 357/459 (77.78%), Postives = 393/459 (85.62%), Query Frame = 1


Query: 10  LSLFALALLFFLT--IALVRKEET-----------SKIRTVSAAEFQSSSNSSMA-TRVE 69
           L + AL  + F    I+L RK +T           + IR +   EFQS ++S+MA TR++
Sbjct: 16  LVVLALTAMIFRNSEISLSRKLKTEVIQSSNSSTMAAIRKLKTEEFQSLNSSTMAATRLD 75

Query: 70  NYDVEQELNNEHAV-DNPD----EIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCD 129
               +Q+    HAV D+PD    E+A  V+MS +N T RRKLGFFSCGTGNPIDDCWRCD
Sbjct: 76  GEPQQQQ----HAVADDPDMVADEVAKLVQMSEQNRTARRKLGFFSCGTGNPIDDCWRCD 135

Query: 130 SNWHRNRKRLAECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWI 189
            NWH+NRKRLA+CGIGFGRNAIGGRDGRFY+VTD +D D VNPKPGTLRHAVIQE+PLWI
Sbjct: 136 RNWHKNRKRLADCGIGFGRNAIGGRDGRFYIVTDPTDEDVVNPKPGTLRHAVIQEEPLWI 195

Query: 190 VFKRDMVIRLKQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGN 249
           VFKRDMVI LKQELIMNSFKTIDARG NVHIANGACITIQF+TN+IIHGLHIHDCKPTGN
Sbjct: 196 VFKRDMVIELKQELIMNSFKTIDARGSNVHIANGACITIQFITNVIIHGLHIHDCKPTGN 255

Query: 250 AMVRSSPSHFGWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHF 309
           AMVRSSPSHFGWRTMADGDA+SIFGSSHIWIDHNSLS+CADGLVDAVMGSTAIT+SNNHF
Sbjct: 256 AMVRSSPSHFGWRTMADGDAVSIFGSSHIWIDHNSLSHCADGLVDAVMGSTAITVSNNHF 315

Query: 310 THHNEVMLLGHSDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYA 369
           THHNEVMLLGHSDSYTKDK MQVTIAYNHFGEGL+QRMPRCRHGYFHVVNNDYTHWEMYA
Sbjct: 316 THHNEVMLLGHSDSYTKDKLMQVTIAYNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMYA 375

Query: 370 IGGSANPTINSQGNRYAAPTNRFAKEVTKRVETPESEWKGWNWRSEGDMLLNGAYFTPSG 429
           IGGSA PTINSQGNRYAAP +RFAKEVTKRVET  SEWK WNWRSEGD+LLNGA+F PSG
Sbjct: 376 IGGSAEPTINSQGNRYAAPMDRFAKEVTKRVETDASEWKKWNWRSEGDLLLNGAFFRPSG 435

Query: 430 AGASASYARASSLGAKSASMVGSITSSAGSLPCRRGHPC 450
           AGASASY RASSL AK +SMV +ITS+AG+L CR+G PC
Sbjct: 436 AGASASYGRASSLAAKPSSMVDTITSTAGALGCRKGRPC 470

[Close]

BLAST of Cucsa.194310 vs. TAIR10
Match: AT1G04680.1 (AT1G04680.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 714.9 bits (1844), Expect = 3.1e-206
Identity = 347/453 (76.60%), Postives = 376/453 (83.00%), Query Frame = 1


Query: 1   MAALTKWSSLSLFALALLFFLTIALVRKEETSKIRTVSAAEFQSSSNSSMATRVENYDVE 60
           MA L  W    L  + LLFF                V A E  +  N S   R +  +  
Sbjct: 1   MAVLPTWL---LAMMCLLFF----------------VGAMENTTHDNISSLPRSDETEW- 60

Query: 61  QELNNEHAVDNPDEIAASV----EMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRN 120
               N+HAV NPDE+A  V    EMS+RN TERRKLG+F+CGTGNPIDDCWRCD NWH+N
Sbjct: 61  ----NQHAVTNPDEVADEVLALTEMSVRNHTERRKLGYFTCGTGNPIDDCWRCDPNWHKN 120

Query: 121 RKRLAECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDM 180
           RKRLA+CGIGFGRNAIGGRDGRFYVVTD  D++PVNP+PGTLRHAVIQ++PLWIVFKRDM
Sbjct: 121 RKRLADCGIGFGRNAIGGRDGRFYVVTDPRDDNPVNPRPGTLRHAVIQDRPLWIVFKRDM 180

Query: 181 VIRLKQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSS 240
           VI+LKQELI+NSFKTID RG NVHIANG CITIQFVTN+I+HGLHIHDCKPTGNAMVRSS
Sbjct: 181 VIQLKQELIVNSFKTIDGRGANVHIANGGCITIQFVTNVIVHGLHIHDCKPTGNAMVRSS 240

Query: 241 PSHFGWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEV 300
            +HFGWRTMADGDAISIFGSSH+WIDHNSLS+CADGLVDAVMGSTAITISNNH THHNEV
Sbjct: 241 ETHFGWRTMADGDAISIFGSSHVWIDHNSLSHCADGLVDAVMGSTAITISNNHLTHHNEV 300

Query: 301 MLLGHSDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSAN 360
           MLLGHSDSY +DK MQVTIAYNHFG GLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSAN
Sbjct: 301 MLLGHSDSYMRDKAMQVTIAYNHFGVGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSAN 360

Query: 361 PTINSQGNRYAAPTNRFAKEVTKRVETPESEWKGWNWRSEGDMLLNGAYFTPSGAGASAS 420
           PTINSQGNRYAAP N FAKEVTKRV+TP S WKGWNWRSEGD+L NGAYFT SGA AS S
Sbjct: 361 PTINSQGNRYAAPKNPFAKEVTKRVDTPASHWKGWNWRSEGDLLQNGAYFTSSGAAASGS 420

Query: 421 YARASSLGAKSASMVGSITSSAGSLPCRRGHPC 450
           YARASSL AKS+S+VG ITS AG+LPCRRG  C
Sbjct: 421 YARASSLSAKSSSLVGHITSDAGALPCRRGRQC 429

[Close]

BLAST of Cucsa.194310 vs. TAIR10
Match: AT3G07010.1 (AT3G07010.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 690.3 bits (1780), Expect = 8.1e-199
Identity = 322/394 (81.73%), Postives = 354/394 (89.85%), Query Frame = 1


Query: 56  NYDVEQELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHR 115
           N     E  +EHAV+NPDE+AA V+MSIRNSTERR+LG+FSC TGNPIDDCWRCD  W  
Sbjct: 23  NASRSNETWHEHAVENPDEVAAMVDMSIRNSTERRRLGYFSCATGNPIDDCWRCDRKWQL 82

Query: 116 NRKRLAECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRD 175
            RKRLA+C IGFGRNAIGGRDGRFYVVTD  D+DPVNP PGTLRHAVIQ++PLWI+FKRD
Sbjct: 83  RRKRLADCSIGFGRNAIGGRDGRFYVVTDPGDDDPVNPIPGTLRHAVIQDEPLWIIFKRD 142

Query: 176 MVIRLKQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRS 235
           MVI LKQELIMNSFKTID RGVNVHIANGAC+TIQ+VTNII+HG+H+HDCKPTGNAMVRS
Sbjct: 143 MVITLKQELIMNSFKTIDGRGVNVHIANGACLTIQYVTNIIVHGIHVHDCKPTGNAMVRS 202

Query: 236 SPSHFGWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNE 295
           SPSH+G+R+MADGDAISIFGSSHIWIDHNSLSNCADGLVDAVM STAIT+SNN FTHHNE
Sbjct: 203 SPSHYGFRSMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMSSTAITVSNNFFTHHNE 262

Query: 296 VMLLGHSDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSA 355
           VMLLGHSDSYT+DK MQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSA
Sbjct: 263 VMLLGHSDSYTRDKVMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSA 322

Query: 356 NPTINSQGNRYAAPTNRFAKEVTKRVETPESEWKGWNWRSEGDMLLNGAYFTPSGAGASA 415
            PTINSQGNR+ AP N FAKEVTKR  T ES+WK WNWRSEGD+ LNGA+FT SGAGA A
Sbjct: 323 GPTINSQGNRFLAPVNPFAKEVTKREYTGESKWKHWNWRSEGDLFLNGAFFTRSGAGAGA 382

Query: 416 SYARASSLGAKSASMVGSITSSAGSLPCRRGHPC 450
           +YARASSL AKS+S+VG++TS +G+L CR G  C
Sbjct: 383 NYARASSLSAKSSSLVGTMTSYSGALNCRAGRRC 416

[Close]

BLAST of Cucsa.194310 vs. TAIR10
Match: AT3G24670.1 (AT3G24670.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 686.0 bits (1769), Expect = 1.5e-197
Identity = 330/435 (75.86%), Postives = 367/435 (84.37%), Query Frame = 1


Query: 15  LALLFFLTIALVRKEETSKIRTVSAAEFQSSSNSSMATRVENYDVEQELNNEHAVDNPDE 74
           LAL   L I  +    ++  +        SSSNSS A ++ N D      NEHAV NP+E
Sbjct: 9   LALSTTLIILALCINSSTMAQETEDLNSHSSSNSSTANKLPNDDGAW---NEHAVKNPEE 68

Query: 75  IAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRNRKRLAECGIGFGRNAIGG 134
           +AA V+M I+NSTERR+LGFFSC TGNPIDDCWRCD NWH  RKRLA C IGFGRNAIGG
Sbjct: 69  VAAMVDMKIKNSTERRRLGFFSCATGNPIDDCWRCDRNWHLRRKRLANCAIGFGRNAIGG 128

Query: 135 RDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIRLKQELIMNSFKTIDA 194
           RDGR+YVVTD SD+D VNP+PGTLRHAVIQ++PLWIVFKRDMVI L QELIMNSFKTID 
Sbjct: 129 RDGRYYVVTDPSDHDAVNPRPGTLRHAVIQDRPLWIVFKRDMVITLTQELIMNSFKTIDG 188

Query: 195 RGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPSHFGWRTMADGDAISIF 254
           RGVNV IA GACITIQ+VTNIIIHG+++HDC+ TGNAMVRSSPSH+GWRTMADGDAISIF
Sbjct: 189 RGVNVAIAGGACITIQYVTNIIIHGINVHDCRRTGNAMVRSSPSHYGWRTMADGDAISIF 248

Query: 255 GSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLGHSDSYTKDKQMQVT 314
           GSSHIWIDHNSLSNCADGL+DA+MGSTAITISNN+ THHNEVML+GHSDSYT+DK MQVT
Sbjct: 249 GSSHIWIDHNSLSNCADGLIDAIMGSTAITISNNYMTHHNEVMLMGHSDSYTRDKLMQVT 308

Query: 315 IAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGNRYAAPTNRFA 374
           IAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHW MYAIGGSANPTINSQGNR+ AP N FA
Sbjct: 309 IAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWVMYAIGGSANPTINSQGNRFLAPGNPFA 368

Query: 375 KEVTKRVETPESEWKGWNWRSEGDMLLNGAYFTPSGAGASASYARASSLGAKSASMVGSI 434
           KEVTKRV + + EWK WNWRS+GD++LNGAYFT SGA A ASYARASSLGAK AS+V  +
Sbjct: 369 KEVTKRVGSWQGEWKQWNWRSQGDLMLNGAYFTKSGAAAPASYARASSLGAKPASVVSML 428

Query: 435 TSSAGSLPCRRGHPC 450
           T S+G+L CR G  C
Sbjct: 429 TYSSGALKCRIGMRC 440

[Close]

BLAST of Cucsa.194310 vs. TAIR10
Match: AT5G48900.1 (AT5G48900.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 683.3 bits (1762), Expect = 9.9e-197
Identity = 317/385 (82.34%), Postives = 349/385 (90.65%), Query Frame = 1


Query: 65  NEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRNRKRLAECG 124
           +EHAV+NP+E+AA V+MSIRNST RR+LG+FSC TGNPIDDCWRCD  W   RK LA C 
Sbjct: 33  HEHAVENPEEVAAMVDMSIRNSTARRRLGYFSCSTGNPIDDCWRCDRRWQSRRKHLANCA 92

Query: 125 IGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIRLKQEL 184
           IGFGRNAIGGRDGR+YVV+D +D++PVNPKPGTLRHAVIQE+PLWIVFKRDMVI LK+EL
Sbjct: 93  IGFGRNAIGGRDGRYYVVSDPNDDNPVNPKPGTLRHAVIQEEPLWIVFKRDMVITLKEEL 152

Query: 185 IMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPSHFGWRT 244
           IMNSFKTID RGVNVHIANGACITIQFVTNIIIHG+HIHDC+PTGNAMVRSSPSH+GWRT
Sbjct: 153 IMNSFKTIDGRGVNVHIANGACITIQFVTNIIIHGIHIHDCRPTGNAMVRSSPSHYGWRT 212

Query: 245 MADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLGHSDS 304
           MADGD ISIFGSSHIWIDHNSLSNCADGL+DAVM STAITISNN+FTHHNEVMLLGHSD+
Sbjct: 213 MADGDGISIFGSSHIWIDHNSLSNCADGLIDAVMASTAITISNNYFTHHNEVMLLGHSDT 272

Query: 305 YTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGN 364
           YT+DK MQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSA+PTINSQGN
Sbjct: 273 YTRDKVMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSASPTINSQGN 332

Query: 365 RYAAPTNRFAKEVTKRVETPESEWKGWNWRSEGDMLLNGAYFTPSGAGASASYARASSLG 424
           RY AP NRFAKEVTKR    + +W+ WNWRSEGD+ LNGA+FT SG+G  ASYARASSL 
Sbjct: 333 RYLAPRNRFAKEVTKRDYAGQWQWRHWNWRSEGDLFLNGAFFTRSGSGLGASYARASSLA 392

Query: 425 AKSASMVGSITSSAGSLPCRRGHPC 450
           AKS+S+VG IT +AG+L CR G  C
Sbjct: 393 AKSSSLVGVITYNAGALNCRGGRRC 417

[Close]

BLAST of Cucsa.194310 vs. NCBI nr
Match: gi|449442407|ref|XP_004138973.1| (PREDICTED: probable pectate lyase 8 isoform X1 [Cucumis sativus])

HSP 1 Score: 927.2 bits (2395), Expect = 1.1e-266
Identity = 449/449 (100.00%), Postives = 449/449 (100.00%), Query Frame = 1


Query: 1   MAALTKWSSLSLFALALLFFLTIALVRKEETSKIRTVSAAEFQSSSNSSMATRVENYDVE 60
           MAALTKWSSLSLFALALLFFLTIALVRKEETSKIRTVSAAEFQSSSNSSMATRVENYDVE
Sbjct: 1   MAALTKWSSLSLFALALLFFLTIALVRKEETSKIRTVSAAEFQSSSNSSMATRVENYDVE 60

Query: 61  QELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRNRKRL 120
           QELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRNRKRL
Sbjct: 61  QELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRNRKRL 120

Query: 121 AECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIRL 180
           AECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIRL
Sbjct: 121 AECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIRL 180

Query: 181 KQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPSHF 240
           KQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPSHF
Sbjct: 181 KQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPSHF 240

Query: 241 GWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLG 300
           GWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLG
Sbjct: 241 GWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLG 300

Query: 301 HSDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTIN 360
           HSDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTIN
Sbjct: 301 HSDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTIN 360

Query: 361 SQGNRYAAPTNRFAKEVTKRVETPESEWKGWNWRSEGDMLLNGAYFTPSGAGASASYARA 420
           SQGNRYAAPTNRFAKEVTKRVETPESEWKGWNWRSEGDMLLNGAYFTPSGAGASASYARA
Sbjct: 361 SQGNRYAAPTNRFAKEVTKRVETPESEWKGWNWRSEGDMLLNGAYFTPSGAGASASYARA 420

Query: 421 SSLGAKSASMVGSITSSAGSLPCRRGHPC 450
           SSLGAKSASMVGSITSSAGSLPCRRGHPC
Sbjct: 421 SSLGAKSASMVGSITSSAGSLPCRRGHPC 449

[Close]

BLAST of Cucsa.194310 vs. NCBI nr
Match: gi|659121573|ref|XP_008460726.1| (PREDICTED: probable pectate lyase 8 isoform X1 [Cucumis melo])

HSP 1 Score: 921.0 bits (2379), Expect = 8.0e-265
Identity = 446/449 (99.33%), Postives = 448/449 (99.78%), Query Frame = 1


Query: 1   MAALTKWSSLSLFALALLFFLTIALVRKEETSKIRTVSAAEFQSSSNSSMATRVENYDVE 60
           MAAL+KWSSLSLFALALLFFLTIALVRKEETSKIRTVSAAEFQSSS+SSMATRVENYDVE
Sbjct: 1   MAALSKWSSLSLFALALLFFLTIALVRKEETSKIRTVSAAEFQSSSDSSMATRVENYDVE 60

Query: 61  QELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRNRKRL 120
           QELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRNRKRL
Sbjct: 61  QELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRNRKRL 120

Query: 121 AECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIRL 180
           AECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIRL
Sbjct: 121 AECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIRL 180

Query: 181 KQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPSHF 240
           KQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPSHF
Sbjct: 181 KQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPSHF 240

Query: 241 GWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLG 300
           GWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLG
Sbjct: 241 GWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLG 300

Query: 301 HSDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTIN 360
           HSDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTIN
Sbjct: 301 HSDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTIN 360

Query: 361 SQGNRYAAPTNRFAKEVTKRVETPESEWKGWNWRSEGDMLLNGAYFTPSGAGASASYARA 420
           SQGNRYAAPTN FAKEVTKRVETPESEWKGWNWRSEGDMLLNGAYFTPSGAGASASYARA
Sbjct: 361 SQGNRYAAPTNPFAKEVTKRVETPESEWKGWNWRSEGDMLLNGAYFTPSGAGASASYARA 420

Query: 421 SSLGAKSASMVGSITSSAGSLPCRRGHPC 450
           SSLGAKSASMVGSITSSAGSLPCRRGHPC
Sbjct: 421 SSLGAKSASMVGSITSSAGSLPCRRGHPC 449

[Close]

BLAST of Cucsa.194310 vs. NCBI nr
Match: gi|778668540|ref|XP_011649115.1| (PREDICTED: probable pectate lyase 1 isoform X2 [Cucumis sativus])

HSP 1 Score: 884.4 bits (2284), Expect = 8.3e-254
Identity = 431/449 (95.99%), Postives = 431/449 (95.99%), Query Frame = 1


Query: 1   MAALTKWSSLSLFALALLFFLTIALVRKEETSKIRTVSAAEFQSSSNSSMATRVENYDVE 60
           MAALTKWSSLSLFALALLFFLTIALVRKEETSKIR                  VENYDVE
Sbjct: 1   MAALTKWSSLSLFALALLFFLTIALVRKEETSKIR------------------VENYDVE 60

Query: 61  QELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRNRKRL 120
           QELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRNRKRL
Sbjct: 61  QELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRNRKRL 120

Query: 121 AECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIRL 180
           AECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIRL
Sbjct: 121 AECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIRL 180

Query: 181 KQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPSHF 240
           KQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPSHF
Sbjct: 181 KQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPSHF 240

Query: 241 GWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLG 300
           GWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLG
Sbjct: 241 GWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLG 300

Query: 301 HSDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTIN 360
           HSDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTIN
Sbjct: 301 HSDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTIN 360

Query: 361 SQGNRYAAPTNRFAKEVTKRVETPESEWKGWNWRSEGDMLLNGAYFTPSGAGASASYARA 420
           SQGNRYAAPTNRFAKEVTKRVETPESEWKGWNWRSEGDMLLNGAYFTPSGAGASASYARA
Sbjct: 361 SQGNRYAAPTNRFAKEVTKRVETPESEWKGWNWRSEGDMLLNGAYFTPSGAGASASYARA 420

Query: 421 SSLGAKSASMVGSITSSAGSLPCRRGHPC 450
           SSLGAKSASMVGSITSSAGSLPCRRGHPC
Sbjct: 421 SSLGAKSASMVGSITSSAGSLPCRRGHPC 431

[Close]

BLAST of Cucsa.194310 vs. NCBI nr
Match: gi|659121575|ref|XP_008460727.1| (PREDICTED: probable pectate lyase 1 isoform X2 [Cucumis melo])

HSP 1 Score: 880.2 bits (2273), Expect = 1.6e-252
Identity = 429/449 (95.55%), Postives = 430/449 (95.77%), Query Frame = 1


Query: 1   MAALTKWSSLSLFALALLFFLTIALVRKEETSKIRTVSAAEFQSSSNSSMATRVENYDVE 60
           MAAL+KWSSLSLFALALLFFLTIALVRKEETSKIR                  VENYDVE
Sbjct: 1   MAALSKWSSLSLFALALLFFLTIALVRKEETSKIR------------------VENYDVE 60

Query: 61  QELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRNRKRL 120
           QELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRNRKRL
Sbjct: 61  QELNNEHAVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRNRKRL 120

Query: 121 AECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIRL 180
           AECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIRL
Sbjct: 121 AECGIGFGRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIRL 180

Query: 181 KQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPSHF 240
           KQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPSHF
Sbjct: 181 KQELIMNSFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPSHF 240

Query: 241 GWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLG 300
           GWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLG
Sbjct: 241 GWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLG 300

Query: 301 HSDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTIN 360
           HSDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTIN
Sbjct: 301 HSDSYTKDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTIN 360

Query: 361 SQGNRYAAPTNRFAKEVTKRVETPESEWKGWNWRSEGDMLLNGAYFTPSGAGASASYARA 420
           SQGNRYAAPTN FAKEVTKRVETPESEWKGWNWRSEGDMLLNGAYFTPSGAGASASYARA
Sbjct: 361 SQGNRYAAPTNPFAKEVTKRVETPESEWKGWNWRSEGDMLLNGAYFTPSGAGASASYARA 420

Query: 421 SSLGAKSASMVGSITSSAGSLPCRRGHPC 450
           SSLGAKSASMVGSITSSAGSLPCRRGHPC
Sbjct: 421 SSLGAKSASMVGSITSSAGSLPCRRGHPC 431

[Close]

BLAST of Cucsa.194310 vs. NCBI nr
Match: gi|1021027419|gb|KZM85206.1| (hypothetical protein DCAR_027372 [Daucus carota subsp. sativus])

HSP 1 Score: 783.5 bits (2022), Expect = 2.0e-223
Identity = 374/442 (84.62%), Postives = 401/442 (90.72%), Query Frame = 1


Query: 9   SLSLFALALLFF-LTIALVRKEETSKIRTVSAAEFQSSSNSSMATRVENYDVEQELNNEH 68
           SLS   L LLF   T  L   +  S I++   AE Q+S NSSMA R+E  DV+ E  NEH
Sbjct: 9   SLSALLLLLLFVGATAVLNNSDHLSSIKSDEKAELQNSVNSSMAARLE--DVDGEGLNEH 68

Query: 69  AVDNPDEIAASVEMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRNRKRLAECGIGF 128
           AV NPDE+A+ V+MSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRNRKRLA+CGIGF
Sbjct: 69  AVSNPDEVASMVDMSIRNSTERRKLGFFSCGTGNPIDDCWRCDSNWHRNRKRLADCGIGF 128

Query: 129 GRNAIGGRDGRFYVVTDSSDNDPVNPKPGTLRHAVIQEKPLWIVFKRDMVIRLKQELIMN 188
           GRNAIGGRDGRFYVVTDS D+DPVNP+PGTLRHAVIQ++PLWIVFKRDMVI+LKQELIMN
Sbjct: 129 GRNAIGGRDGRFYVVTDSRDDDPVNPRPGTLRHAVIQDRPLWIVFKRDMVIQLKQELIMN 188

Query: 189 SFKTIDARGVNVHIANGACITIQFVTNIIIHGLHIHDCKPTGNAMVRSSPSHFGWRTMAD 248
           SFKTID RGVNVHIANG CITIQFVTN+IIHGLHIHDCKPTGNAMVRSSP+HFGWRTMAD
Sbjct: 189 SFKTIDGRGVNVHIANGGCITIQFVTNVIIHGLHIHDCKPTGNAMVRSSPTHFGWRTMAD 248

Query: 249 GDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNHFTHHNEVMLLGHSDSYTK 308
           GDAISIFGSSH+W+DHNSLSNCADGLVDAVMGSTAIT+SNNHFTHHNEVMLLGHSDSYT+
Sbjct: 249 GDAISIFGSSHVWVDHNSLSNCADGLVDAVMGSTAITVSNNHFTHHNEVMLLGHSDSYTR 308

Query: 309 DKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGNRYA 368
           DKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSA+PTINSQGNRY 
Sbjct: 309 DKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSASPTINSQGNRYL 368

Query: 369 APTNRFAKEVTKRVETPESEWKGWNWRSEGDMLLNGAYFTPSGAGASASYARASSLGAKS 428
           AP N FAKEVTKRVETP   WKGWNWRSEGD+LLNGAYFTPSGAGA++SYARASSLGAKS
Sbjct: 369 APANPFAKEVTKRVETPVGHWKGWNWRSEGDLLLNGAYFTPSGAGATSSYARASSLGAKS 428

Query: 429 ASMVGSITSSAGSLPCRRGHPC 450
           +SMVG+ITS AG L CRRGH C
Sbjct: 429 SSMVGTITSGAGVLGCRRGHQC 448

The following BLAST results are available for this feature:

BLAST of Cucsa.194310 vs. Swiss-Prot


Match Name	E-value	Identity	Description
PLY15_ARATH	3.1e-208	77.78	Probable pectate lyase 15 OS=Arabidopsis thaliana GN=At4g13710 PE=2 SV=1	[more]
PLY1_ARATH	5.5e-205	76.60	Probable pectate lyase 1 OS=Arabidopsis thaliana GN=At1g04680 PE=2 SV=2	[more]
PLY8_ARATH	1.4e-197	81.73	Probable pectate lyase 8 OS=Arabidopsis thaliana GN=At3g07010 PE=2 SV=1	[more]
PLY10_ARATH	2.7e-196	75.86	Probable pectate lyase 10 OS=Arabidopsis thaliana GN=At3g24670 PE=2 SV=2	[more]
PLY20_ARATH	1.8e-195	82.34	Probable pectate lyase 20 OS=Arabidopsis thaliana GN=At5g48900 PE=2 SV=1	[more]

BLAST of Cucsa.194310 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LI24_CUCSA	7.8e-267	100.00	Pectate lyase OS=Cucumis sativus GN=Csa_2G155600 PE=3 SV=1	[more]
D4NXR8_PRUPE	1.7e-221	83.33	Pectate lyase OS=Prunus persica GN=PRUPE_ppa005761mg PE=2 SV=1	[more]
A1IIA8_PRUPE	1.1e-220	87.14	Pectate lyase (Fragment) OS=Prunus persica GN=PpPL2 PE=2 SV=1	[more]
A0A0A0L814_CUCSA	4.6e-219	82.18	Pectate lyase OS=Cucumis sativus GN=Csa_3G133180 PE=3 SV=1	[more]
A0A0D2S7U3_GOSRA	3.0e-218	81.90	Pectate lyase OS=Gossypium raimondii GN=B456_006G219800 PE=3 SV=1	[more]

BLAST of Cucsa.194310 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G13710.1	1.7e-209	77.78	Pectin lyase-like superfamily protein	[more]
AT1G04680.1	3.1e-206	76.60	Pectin lyase-like superfamily protein	[more]
AT3G07010.1	8.1e-199	81.73	Pectin lyase-like superfamily protein	[more]
AT3G24670.1	1.5e-197	75.86	Pectin lyase-like superfamily protein	[more]
AT5G48900.1	9.9e-197	82.34	Pectin lyase-like superfamily protein	[more]

BLAST of Cucsa.194310 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449442407\|ref\|XP_004138973.1\|	1.1e-266	100.00	PREDICTED: probable pectate lyase 8 isoform X1 [Cucumis sativus]	[more]
gi\|659121573\|ref\|XP_008460726.1\|	8.0e-265	99.33	PREDICTED: probable pectate lyase 8 isoform X1 [Cucumis melo]	[more]
gi\|778668540\|ref\|XP_011649115.1\|	8.3e-254	95.99	PREDICTED: probable pectate lyase 1 isoform X2 [Cucumis sativus]	[more]
gi\|659121575\|ref\|XP_008460727.1\|	1.6e-252	95.55	PREDICTED: probable pectate lyase 1 isoform X2 [Cucumis melo]	[more]
gi\|1021027419\|gb\|KZM85206.1\|	2.0e-223	84.62	hypothetical protein DCAR_027372 [Daucus carota subsp. sativus]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR002022	Pec_lyase
IPR011050	Pectin_lyase_fold/virulence
IPR012334	Pectin_lyas_fold
IPR018082	AmbAllergen

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0045490	pectin catabolic process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0030570	pectate lyase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsa.194310.1	Cucsa.194310.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002022	Pectate lyase/Amb allergen	PFAM	PF00544	Pec_lyase_C	coord: 187..364 score: 2.5
IPR002022	Pectate lyase/Amb allergen	SMART	SM00656	amb_all	coord: 175..372 score: 1.3E
IPR011050	Pectin lyase fold/virulence factor	unknown	SSF51126	Pectin lyase-like	coord: 77..443 score: 9.26E
IPR012334	Pectin lyase fold	GENE3D	G3DSA:2.160.20.10		coord: 101..440 score: 1.8E
IPR018082	AmbAllergen	PRINTS	PR00807	AMBALLERGEN	coord: 183..199 score: 5.2E-81coord: 391..415 score: 5.2E-81coord: 349..368 score: 5.2E-81coord: 420..443 score: 5.2E-81coord: 247..268 score: 5.2E-81coord: 123..140 score: 5.2E-81coord: 147..172 score: 5.2E-81coord: 327..346 score: 5.2
None	No IPR available	PANTHER	PTHR31683	FAMILY NOT NAMED	coord: 29..449 score:
None	No IPR available	PANTHER	PTHR31683:SF20	PECTATE LYASE 1-RELATED	coord: 29..449 score:

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Cucsa.194310	Csa2G155600	Cucumber (Chinese Long) v2	cgycuB296
Cucsa.194310	MELO3C023087	Melon (DHL92) v3.5.1	cgymeB352
Cucsa.194310	CSPI02G09630	Wild cucumber (PI 183967)	cgycpiB305
Cucsa.194310	MELO3C023087.2	Melon (DHL92) v3.6.1	cgymedB347
Cucsa.194310	CsaV3_2G012050	Cucumber (Chinese Long) v3	cgycucB309

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:

None